AR125455A1 - Modulación de la calidad de producto de anticuerpos multiespecíficos asimétricos a través del uso de la temperatura - Google Patents
Modulación de la calidad de producto de anticuerpos multiespecíficos asimétricos a través del uso de la temperaturaInfo
- Publication number
- AR125455A1 AR125455A1 ARP220101081A ARP220101081A AR125455A1 AR 125455 A1 AR125455 A1 AR 125455A1 AR P220101081 A ARP220101081 A AR P220101081A AR P220101081 A ARP220101081 A AR P220101081A AR 125455 A1 AR125455 A1 AR 125455A1
- Authority
- AR
- Argentina
- Prior art keywords
- asymmetric
- temperature
- multispecific antibody
- temperature regime
- cell culture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2818—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invención se refiere al campo de la fabricación de biofármacos. En particular, la invención se refiere al uso de la temperatura como palanca para modular la calidad de producto durante las operaciones corriente arriba. Reivindicación 1: Un método para modular la calidad de producto de un anticuerpo multiespecífico asimétrico recombinante expresado por una célula durante el cultivo celular que comprende las etapas: a) establecer al menos dos cultivos celulares, cada uno inoculado con la misma estirpe celular que expresa el anticuerpo multiespecífico asimétrico; b) cultivar al menos un cultivo celular en un primer régimen de temperatura que consiste en una única temperatura y al menos un cultivo celular en un segundo régimen de temperatura; c) comparar al menos una impureza relacionada con el producto en el cultivo celular en cada régimen de temperatura; d) seleccionar el régimen de temperatura que reduce la expresión de al menos una impureza relacionada con el producto que comprende una cadena pesada larga no emparejada o emparejada erróneamente; e) cultivar la estirpe celular en el régimen de temperatura seleccionado; y f) recoger el anticuerpo multiespecífico asimétrico recombinante. Reivindicación 10: El método de acuerdo con la reivindicación 1, en donde el régimen de temperatura de cultivo celular puede seleccionarse además para la modulación de la cantidad de expresión, la productividad, el crecimiento, el rendimiento y/u otros atributos de calidad de producto deseados del anticuerpo multiespecífico asimétrico. Reivindicación 11: El método de acuerdo con la reivindicación 1, en donde el anticuerpo multiespecífico asimétrico comprende una muteína.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163180220P | 2021-04-27 | 2021-04-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR125455A1 true AR125455A1 (es) | 2023-07-19 |
Family
ID=81654591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220101081A AR125455A1 (es) | 2021-04-27 | 2022-04-26 | Modulación de la calidad de producto de anticuerpos multiespecíficos asimétricos a través del uso de la temperatura |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4330280A1 (es) |
| JP (1) | JP2024516401A (es) |
| AR (1) | AR125455A1 (es) |
| AU (1) | AU2022263986A1 (es) |
| CA (1) | CA3217850A1 (es) |
| TW (1) | TW202309081A (es) |
| WO (1) | WO2022232083A1 (es) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2447791A1 (en) * | 2001-06-13 | 2002-12-19 | Neslihan Delacruz | Methods of culturing animal cells and polypeptide production in animal cells |
| EP3916011A1 (en) | 2009-06-26 | 2021-12-01 | Regeneron Pharmaceuticals, Inc. | Readily isolated bispecific antibodies with native immunoglobulin format |
| AU2018311079B2 (en) | 2017-08-03 | 2022-06-23 | Amgen Inc. | Interleukin-21 muteins and methods of treatment |
| JP2019122373A (ja) | 2018-01-12 | 2019-07-25 | アムジエン・インコーポレーテツド | 抗ｐｄ−１抗体及び治療方法 |
-
2022
- 2022-04-26 AU AU2022263986A patent/AU2022263986A1/en active Pending
- 2022-04-26 WO PCT/US2022/026261 patent/WO2022232083A1/en active Application Filing
- 2022-04-26 EP EP22723876.3A patent/EP4330280A1/en active Pending
- 2022-04-26 AR ARP220101081A patent/AR125455A1/es unknown
- 2022-04-26 TW TW111115759A patent/TW202309081A/zh unknown
- 2022-04-26 CA CA3217850A patent/CA3217850A1/en active Pending
- 2022-04-26 JP JP2023565519A patent/JP2024516401A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024516401A (ja) | 2024-04-15 |
| AU2022263986A1 (en) | 2023-11-09 |
| WO2022232083A1 (en) | 2022-11-03 |
| CA3217850A1 (en) | 2022-11-03 |
| WO2022232083A9 (en) | 2023-11-30 |
| TW202309081A (zh) | 2023-03-01 |
| EP4330280A1 (en) | 2024-03-06 |
| AU2022263986A9 (en) | 2023-11-16 |
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