AR109860A1 - Producción acelerada de callos embriogénicos, embriones somáticos y métodos de transformación relacionados - Google Patents
Producción acelerada de callos embriogénicos, embriones somáticos y métodos de transformación relacionadosInfo
- Publication number
- AR109860A1 AR109860A1 ARP170102936A ARP170102936A AR109860A1 AR 109860 A1 AR109860 A1 AR 109860A1 AR P170102936 A ARP170102936 A AR P170102936A AR P170102936 A ARP170102936 A AR P170102936A AR 109860 A1 AR109860 A1 AR 109860A1
- Authority
- AR
- Argentina
- Prior art keywords
- embryos
- immature
- somatic
- soy
- somatic embryos
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/425—Zeins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Physiology (AREA)
Abstract
Se proporciona un método para la producción de callos embriogénicos de soja y embriones somáticos. Se proporciona además métodos para transformar explantes que incluyen generar tejido de embrión somático. Los embriones somáticos transgénicos producidos pueden usarse para regenerar plantas transgénicas estables. Reivindicación 1: Un método para la producción de callos embriogénicos, donde el método comprende: seleccionar uno o más embriones de soja inmaduros; someter uno o más embriones inmaduros a tratamiento en frío; e inducir los embriones tratados en frío a formar callos embriogénicos. Reivindicación 13: Un método para la transformación de soja, donde el método comprende: seleccionar múltiples embriones de soja inmaduros; someter los embriones inmaduros a tratamiento en frío; transformar los embriones cigóticos inmaduros tratados en frío con uno o más transgenes exógenos; e inducir los embriones cigóticos transformados a formar embriones somáticos y producir así tejido de embrión somático transgénico.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662410526P | 2016-10-20 | 2016-10-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR109860A1 true AR109860A1 (es) | 2019-01-30 |
Family
ID=61970883
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP170102936A AR109860A1 (es) | 2016-10-20 | 2017-10-20 | Producción acelerada de callos embriogénicos, embriones somáticos y métodos de transformación relacionados |
Country Status (7)
Country | Link |
---|---|
US (1) | US20180110196A1 (es) |
EP (1) | EP3528610A1 (es) |
AR (1) | AR109860A1 (es) |
BR (1) | BR112019006760A2 (es) |
CA (1) | CA3039205A1 (es) |
TW (1) | TW201815278A (es) |
WO (1) | WO2018075602A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3732295A1 (en) * | 2017-12-27 | 2020-11-04 | Pioneer Hi-Bred International, Inc. | Transformation of dicot plants |
CN110241069A (zh) * | 2019-06-04 | 2019-09-17 | 上海交通大学 | 一种大豆原生质体的制备和转化方法 |
CN116262931A (zh) * | 2021-12-14 | 2023-06-16 | 中国科学院分子植物科学卓越创新中心 | 一种黍属植物的遗传转化方法 |
CN116548307B (zh) * | 2023-05-12 | 2024-01-26 | 青岛农业大学 | 一种基于叶片诱导的紫花苜蓿再生的方法及其培养基 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5024944A (en) * | 1986-08-04 | 1991-06-18 | Lubrizol Genetics, Inc. | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species |
US4857465A (en) * | 1986-12-02 | 1989-08-15 | Lubrizol Genetics, Inc. | Whole plant regeneration via organogenesis and somaclonal variation in glycine species |
CA2396392C (en) * | 1999-11-29 | 2015-04-21 | Midwest Oilseeds, Inc. | Methods and compositions for the introduction of molecules into cells |
EP3051940A4 (en) * | 2013-10-04 | 2017-03-29 | Dow AgroSciences LLC | Soybean transformation method |
-
2017
- 2017-10-16 TW TW106135319A patent/TW201815278A/zh unknown
- 2017-10-18 EP EP17861539.9A patent/EP3528610A1/en not_active Withdrawn
- 2017-10-18 US US15/786,916 patent/US20180110196A1/en not_active Abandoned
- 2017-10-18 BR BR112019006760A patent/BR112019006760A2/pt not_active Application Discontinuation
- 2017-10-18 WO PCT/US2017/057120 patent/WO2018075602A1/en unknown
- 2017-10-18 CA CA3039205A patent/CA3039205A1/en not_active Abandoned
- 2017-10-20 AR ARP170102936A patent/AR109860A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
BR112019006760A2 (pt) | 2019-07-02 |
WO2018075602A1 (en) | 2018-04-26 |
EP3528610A1 (en) | 2019-08-28 |
TW201815278A (zh) | 2018-05-01 |
US20180110196A1 (en) | 2018-04-26 |
CA3039205A1 (en) | 2018-04-26 |
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