BR112019006760A2 - produção acelerada de calos embriogênicos, embriões somáticos e métodos de transformação relacionados - Google Patents

produção acelerada de calos embriogênicos, embriões somáticos e métodos de transformação relacionados

Info

Publication number
BR112019006760A2
BR112019006760A2 BR112019006760A BR112019006760A BR112019006760A2 BR 112019006760 A2 BR112019006760 A2 BR 112019006760A2 BR 112019006760 A BR112019006760 A BR 112019006760A BR 112019006760 A BR112019006760 A BR 112019006760A BR 112019006760 A2 BR112019006760 A2 BR 112019006760A2
Authority
BR
Brazil
Prior art keywords
somatic embryos
transformation methods
embryogenic callus
accelerated production
related transformation
Prior art date
Application number
BR112019006760A
Other languages
English (en)
Inventor
Pareddy Dayakar
K Effinger Katherine
Sarria Rodrigo
Reddy Chennareddy Sivarama
Kumar Mall Tejinder
M Tobias (Toby) Cicak
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112019006760A2 publication Critical patent/BR112019006760A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture

Abstract

a presente invenção refere-se a um método para a produção de calo embriogênico e embriões somáticos de soja. a invenção proporciona ainda métodos de transformação de explantes, os quais incluem a geração de tecido de embriões somáticos. os embriões somáticos transgênicos produzidos podem ser usados para regenerar plantas transgênicas estáveis.
BR112019006760A 2016-10-20 2017-10-18 produção acelerada de calos embriogênicos, embriões somáticos e métodos de transformação relacionados BR112019006760A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410526P 2016-10-20 2016-10-20
PCT/US2017/057120 WO2018075602A1 (en) 2016-10-20 2017-10-18 Accelerated production of embryogenic callus, somatic embryos, and related transformation methods

Publications (1)

Publication Number Publication Date
BR112019006760A2 true BR112019006760A2 (pt) 2019-07-02

Family

ID=61970883

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019006760A BR112019006760A2 (pt) 2016-10-20 2017-10-18 produção acelerada de calos embriogênicos, embriões somáticos e métodos de transformação relacionados

Country Status (7)

Country Link
US (1) US20180110196A1 (pt)
EP (1) EP3528610A1 (pt)
AR (1) AR109860A1 (pt)
BR (1) BR112019006760A2 (pt)
CA (1) CA3039205A1 (pt)
TW (1) TW201815278A (pt)
WO (1) WO2018075602A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019133371A1 (en) * 2017-12-27 2019-07-04 Pioneer Hi-Bred International, Inc. Transformation of dicot plants
CN110241069A (zh) * 2019-06-04 2019-09-17 上海交通大学 一种大豆原生质体的制备和转化方法
CN116262931A (zh) * 2021-12-14 2023-06-16 中国科学院分子植物科学卓越创新中心 一种黍属植物的遗传转化方法
CN116548307B (zh) * 2023-05-12 2024-01-26 青岛农业大学 一种基于叶片诱导的紫花苜蓿再生的方法及其培养基

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5024944A (en) * 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US4857465A (en) * 1986-12-02 1989-08-15 Lubrizol Genetics, Inc. Whole plant regeneration via organogenesis and somaclonal variation in glycine species
EP1250447B1 (en) * 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
CN105792642A (zh) * 2013-10-04 2016-07-20 美国陶氏益农公司 大豆转化方法

Also Published As

Publication number Publication date
EP3528610A1 (en) 2019-08-28
CA3039205A1 (en) 2018-04-26
WO2018075602A1 (en) 2018-04-26
US20180110196A1 (en) 2018-04-26
TW201815278A (zh) 2018-05-01
AR109860A1 (es) 2019-01-30

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]