AR099371A1 - Plantas que tienen una mayor tolerancia a herbicidas - Google Patents

Plantas que tienen una mayor tolerancia a herbicidas

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Publication number
AR099371A1
AR099371A1 ARP140104756A ARP140104756A AR099371A1 AR 099371 A1 AR099371 A1 AR 099371A1 AR P140104756 A ARP140104756 A AR P140104756A AR P140104756 A ARP140104756 A AR P140104756A AR 099371 A1 AR099371 A1 AR 099371A1
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AR
Argentina
Prior art keywords
plant
mutated
seq
site
ppo
Prior art date
Application number
ARP140104756A
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English (en)
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Basf Agro Bv
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Publication date
Application filed by Basf Agro Bv filed Critical Basf Agro Bv
Publication of AR099371A1 publication Critical patent/AR099371A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/84—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catching Or Destruction (AREA)

Abstract

Reivindicación 1: Un método para controlar la vegetación no deseada en un sitio de cultivo vegetal; el método comprende las siguientes etapas: a) proveer, en el sitio, una planta que comprende al menos un ácido nucleico que comprende una secuencia de nucleótidos que codifica una protoporfirinógeno oxidasa de tipo silvestre o una protoporfirinógeno oxidasa mutada (PPO mutada) que es resistente o tolerante a un herbicida inhibidor de PPO, y que comprende la secuencia de aminoácidos de SEQ ID Nº 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116 ó 117, o una variante de aquella; b) aplicar al sitio una cantidad eficaz del herbicida. Reivindicación 5: Una célula vegetal transgénica que comprende un ácido nucleico que codifica un polipéptido PPO mutado, en donde la expresión del ácido nucleico en la célula vegetal da como resultado una mayor resistencia o tolerancia a un herbicida inhibidor de PPO en comparación con una variedad de tipo silvestre de la célula vegetal, y en donde la PPO mutada codificada es una variante de SEQ ID Nº 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116 ó 117. Reivindicación 7: Una planta que expresa un polipéptido PPO mutado mutagenizado o recombinante que comprende la secuencia de SEQ ID Nº 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116 ó 117, una variante, un derivado, un ortólogo, un parálogo o un homólogo de aquella, en donde el aminoácido en el sitio mutado 1 que corresponde a Arg128 de SEQ ID Nº 1 es Leu, Ala, Val, lle, Met, Tyr, Gly, Asn, Cys, Phe, Ser, Thr, Gln o His; y/o el aminoácido en el sitio mutado 4 que corresponde a Leu397 de SEQ ID Nº 1 es Ala, Arg, Val, lle, Met, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Gly, Pro, Phe, Tyr o Trp; y/o el aminoácido en el sitio mutado 5 que corresponde a Phe420 de SEQ ID Nº 1 es Ala, Leu, Val, lle, Met, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Gly, Pro, Arg, Tyr o Trp, y en donde dicho PPO mutado confiere a la planta una mayor tolerancia a herbicidas, en comparación con la variedad de tipo silvestre correspondiente de la planta cuando se expresa en esta. Reivindicación 10: Un método para producir una planta transgénica, que comprende (a) transformar una célula vegetal con un casete de expresión que comprende un ácido nucleico que codifica un polipéptido PPO mutado que comprende la secuencia de SEQ ID Nº 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116 ó 117, una variante, un derivado, un ortólogo, un parálogo o un homólogo de aquella, y (b) generar una planta con una mayor resistencia a un herbicida inhibidor de PPO de la célula vegetal.
ARP140104756A 2013-12-18 2014-12-18 Plantas que tienen una mayor tolerancia a herbicidas AR099371A1 (es)

Applications Claiming Priority (1)

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US201361917360P 2013-12-18 2013-12-18

Publications (1)

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AR099371A1 true AR099371A1 (es) 2016-07-20

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ARP140104756A AR099371A1 (es) 2013-12-18 2014-12-18 Plantas que tienen una mayor tolerancia a herbicidas

Country Status (12)

Country Link
US (3) US10308953B2 (es)
EP (1) EP3083968A4 (es)
CN (1) CN106029890B (es)
AR (1) AR099371A1 (es)
AU (3) AU2014369229A1 (es)
BR (1) BR112016014174B1 (es)
CA (1) CA2933002C (es)
EA (1) EA201691198A1 (es)
MD (1) MD4770B1 (es)
PH (1) PH12016501133A1 (es)
UY (1) UY35891A (es)
WO (1) WO2015092706A1 (es)

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CN116064434A (zh) * 2016-06-16 2023-05-05 福阿母韩农株式会社 原卟啉原氧化酶变体
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CN109790153A (zh) 2016-07-27 2019-05-21 巴斯夫农业公司 具有增加的除草剂耐受性的植物
CN109475099A (zh) * 2016-07-29 2019-03-15 孟山都技术公司 用于在植物中进行基因表达的方法和组合物
BR112019012763A2 (pt) * 2016-12-20 2019-12-10 Basf Agro Bv vegetais ou partes de vegetais, semente capaz de germinação em um vegetal, célula de vegetal ou capaz de regenerar um vegetal, célula de vegetal, produto de vegetal, progênie ou vegetal descendente, métodos para o controle de ervas daninhas e a produção de um vegetal, molécula de ácido nucléico, cassete de expressão, vetor, polipeptídeo de ppo mutada isolado, método para a produção de um produto de vegetal e produto de vegetal
EP3716765A4 (en) * 2017-11-29 2021-08-25 Basf Se PLANTS WITH INCREASED TOLERANCE TO HERBICIDES
AU2018385130B2 (en) * 2017-12-15 2022-07-14 Farmhannong Co., Ltd. Composition and method for conferring and/or enhancing herbicide tolerance using variants of protoporphyrinogen ix oxidase from cyanobacteria
CN111727245A (zh) * 2017-12-15 2020-09-29 福阿母韩农株式会社 使用ppo变体赋予和/或增强对除草剂的耐受性的组合物和方法
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BR112020012211A2 (pt) * 2017-12-20 2020-11-24 Sumitomo Chemical Company, Limited método para controlar organismos nocivos em safras
US20230175004A1 (en) * 2019-06-14 2023-06-08 Farmhannong Co., Ltd. Methods and compositions for conferring and/or enhancing herbicide tolerance using protoporphyrinogen ix oxidase of various cyanobacteria or variant thereof
CN111777453B (zh) * 2020-07-01 2022-05-20 浙江省农业科学院 一种提高青花菜花球中萝卜硫苷含量营养液组合物和方法
CA3197526A1 (en) 2020-11-24 2022-06-02 William Guy Whittingham Herbicidal compounds
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KR20230141283A (ko) * 2022-03-31 2023-10-10 주식회사 팜한농 프로토포르피리노겐 옥시다아제의 변이체를 이용하는 트리플루디목사진 내성 부여 및/또는 증진을 위한 조성물 및 방법
WO2023222589A1 (en) 2022-05-20 2023-11-23 Syngenta Crop Protection Ag Herbicidal compounds
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