AR098490A1 - Mutantes del gen que codifica la enzima fenilacetona monooxigenasa (pamo) - Google Patents

Mutantes del gen que codifica la enzima fenilacetona monooxigenasa (pamo)

Info

Publication number
AR098490A1
AR098490A1 ARP140104366A ARP140104366A AR098490A1 AR 098490 A1 AR098490 A1 AR 098490A1 AR P140104366 A ARP140104366 A AR P140104366A AR P140104366 A ARP140104366 A AR P140104366A AR 098490 A1 AR098490 A1 AR 098490A1
Authority
AR
Argentina
Prior art keywords
pamo
mutants
enzyme
phenylacetone
caprolactone
Prior art date
Application number
ARP140104366A
Other languages
English (en)
Original Assignee
Pontificia Univ Católica De Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pontificia Univ Católica De Chile filed Critical Pontificia Univ Católica De Chile
Publication of AR098490A1 publication Critical patent/AR098490A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13092—Phenylacetone monooxygenase (1.14.13.92)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/08—Oxygen as only ring hetero atoms containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se refiere a mutantes del gen que codifica para la enzima fenilacetona monooxigenasa (PAMO) que fue aislada a partir de la bacteria de nombre científico Thermofibida fusca o Thermomonospora fusca con sustituciones en las posiciones 93, 94 y 440 (N, D y F, respectivamente) de la secuencia original de amino ácidos, y combinaciones de sustituciones especificas de las posiciones 441, 442, 443 y/o 444 (G o D, P o E, T, V, I o W y Q, respectivamente), las que muestran tanto un alto rendimiento como catalizadores en la conversión de ciclohexanona a e-caprolactona, como también alta estabilidad térmica. ADN codificante para dichas enzimas y secuencias, amino acídicas codificadas, las cuales se proponen como biocatalizadores para un proceso de conversión más eficiente y con menor generación de residuos tóxicos comparado con los procesos industriales existentes de producción de e-caprolactona.
ARP140104366A 2013-11-22 2014-11-20 Mutantes del gen que codifica la enzima fenilacetona monooxigenasa (pamo) AR098490A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2013003356A CL2013003356A1 (es) 2013-11-22 2013-11-22 Mutantes del gen que codifica la enzina fenilacetona monooxigenasa (pamo) aislada de termofibida fusca o thermononospora fusca con sustitucines en las posiciones 93,94,y 440 (n,d y f, respectivamente) y combinaciones especificas de las posiciones 441,442,443 y/o 444(g o d,p o e,t,v,i o w y q respectivamente ) con alto rendimiento como catalizadores en la conversion de ciclohexanona a epailon-caprolactona y alta estabilidad termica , adn condificante y secuencias amino acidicas codificadas.

Publications (1)

Publication Number Publication Date
AR098490A1 true AR098490A1 (es) 2016-06-01

Family

ID=53178765

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140104366A AR098490A1 (es) 2013-11-22 2014-11-20 Mutantes del gen que codifica la enzima fenilacetona monooxigenasa (pamo)

Country Status (3)

Country Link
AR (1) AR098490A1 (es)
CL (1) CL2013003356A1 (es)
WO (1) WO2015074162A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113430216B (zh) * 2021-07-26 2023-07-21 福州大学 一种苯丙酮单加氧酶及在拉唑类药物制备中的应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995012668A1 (en) 1993-11-05 1995-05-11 Cornell Research Foundation, Inc. Thermostable xylanase from a thermomonospora fusca gene
US5677151A (en) 1994-06-24 1997-10-14 Cornell Research Foundation, Inc. Thermostable cellulase from a thermomonospora gene
US5871550A (en) 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
US6365376B1 (en) 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US6995005B1 (en) 1999-09-30 2006-02-07 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf) Enzyme which cleaves ester groups and which is derived from Thermononospora fusca
ATE359122T1 (de) 2000-01-31 2007-05-15 Solvay Verfahren zur oxidation mit einem katalysator von einer antimontrifluorid und siliziumdioxid enthaltenden zusammensatzung
US6838577B2 (en) 2000-08-24 2005-01-04 Union Carbide Chemicals & Plastics Technology Corporation Processes for the manufacture of lactones
US7105296B2 (en) 2001-08-29 2006-09-12 E. I. Du Pont De Nemours And Company Genes encoding Baeyer-Villiger monooxygenases
DE10308489A1 (de) 2003-02-26 2004-09-09 Basf Ag Verfahren zur Herstellung von Cyclopentanon und Caprolacton aus Dicarbonsäure-Lösung
JP2008247889A (ja) 2007-03-05 2008-10-16 Sumitomo Chemical Co Ltd ε−カプロラクトン化合物の製造方法
US7666751B2 (en) 2007-09-21 2010-02-23 Semiconductor Components Industries, Llc Method of forming a high capacitance diode and structure therefor
CN102260655B (zh) 2009-12-18 2012-07-25 江南大学 一种角质酶的突变体及其制备方法
CN102260634B (zh) 2010-05-28 2012-11-21 中国科学院成都生物研究所 一株高效甲醛降解菌及其用途和使用方法
US20130150551A1 (en) 2010-06-16 2013-06-13 Bioamber S.A.S. Processes for the production of hydrogenated products and derivatives thereof
WO2012032002A2 (de) 2010-09-08 2012-03-15 Basf Se VERFAHREN ZUR HERSTELLUNG VON ε-CAPROLACTON UND 1,6-HEXANDIOL
EP2636665B1 (en) 2010-11-02 2016-06-22 National University Corporation Nagoya University Method for producing ester
BR112014010448A2 (pt) 2011-11-02 2017-04-18 Genomatica Inc microorganismos e métodos para a produção de caprolactona
CN102443578B (zh) 2011-12-08 2013-10-16 江南大学 一种葡萄糖异构酶突变体及其应用

Also Published As

Publication number Publication date
CL2013003356A1 (es) 2014-08-08
WO2015074162A1 (es) 2015-05-28

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