AR095418A1 - Métodos para aumentar el contenido de manosa en proteínas recombinantes - Google Patents

Métodos para aumentar el contenido de manosa en proteínas recombinantes

Info

Publication number
AR095418A1
AR095418A1 ARP140100962A ARP140100962A AR095418A1 AR 095418 A1 AR095418 A1 AR 095418A1 AR P140100962 A ARP140100962 A AR P140100962A AR P140100962 A ARP140100962 A AR P140100962A AR 095418 A1 AR095418 A1 AR 095418A1
Authority
AR
Argentina
Prior art keywords
manose
methods
content
increase
recombinant proteins
Prior art date
Application number
ARP140100962A
Other languages
English (en)
Inventor
R Lindahl Katherine
C Brandestein Michael
Erik Lillie Shawn
P Davern Sean
Wu Jian
Crina Petrovan Slmina
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50397323&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR095418(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AR095418A1 publication Critical patent/AR095418A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Un método para modular una o más especies de glicano de alto contenido de manosa sobre una proteína recombinante durante un proceso de cultivo de células de mamífero caracterizado porque comprende limitar la cantidad de glucosa en el medio de cultivo celular y suplementar el medio de cultivo celular con galactosa o sacarosa.
ARP140100962A 2013-03-14 2014-03-13 Métodos para aumentar el contenido de manosa en proteínas recombinantes AR095418A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361784639P 2013-03-14 2013-03-14

Publications (1)

Publication Number Publication Date
AR095418A1 true AR095418A1 (es) 2015-10-14

Family

ID=50397323

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140100962A AR095418A1 (es) 2013-03-14 2014-03-13 Métodos para aumentar el contenido de manosa en proteínas recombinantes

Country Status (17)

Country Link
US (6) US9822388B2 (es)
EP (3) EP3434760B1 (es)
AR (1) AR095418A1 (es)
CY (1) CY1120824T1 (es)
DK (2) DK2970874T3 (es)
ES (2) ES2691801T3 (es)
FI (1) FI3434760T3 (es)
HR (1) HRP20230667T1 (es)
HU (2) HUE040682T2 (es)
LT (2) LT2970874T (es)
PL (2) PL3434760T3 (es)
PT (2) PT2970874T (es)
RS (1) RS64326B1 (es)
SI (2) SI3434760T1 (es)
TR (1) TR201815892T4 (es)
TW (4) TWI625390B (es)
WO (1) WO2014159259A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
ES2625045T3 (es) 2011-07-01 2017-07-18 Amgen Inc. Cultivo de células de mamífero
US10059770B2 (en) 2012-01-30 2018-08-28 Dr. Reddy's Laboratories Limited Process of modulating man5 and/or afucosylation content of a glycoprotein composition
TWI625390B (zh) 2013-03-14 2018-06-01 安美基公司 用於增加重組蛋白質之甘露糖含量之方法
MX2016005572A (es) * 2013-10-31 2016-12-09 Amgen Inc Uso de monensina para regular la glicosilacion de proteinas recombinantes.
AU2016374304A1 (en) * 2015-12-15 2018-06-14 CSL Behring Lengnau AG Method for increasing the heterogeneity of O-glycosylation of recombinant factor VII
EP3585880A1 (en) 2017-02-27 2020-01-01 Life Technologies Corporation Expansion of populations of t cells by the use of modified serum free media
WO2019094333A1 (en) * 2017-11-13 2019-05-16 Amgen Inc. Methods for producing protein products
JP7267284B2 (ja) 2017-12-20 2023-05-01 アレス トレーディング ソシエテ アノニム ポリエーテルイオノフォアを使用するタンパク質マンノシル化プロファイルの調節方法
KR20210005689A (ko) * 2018-05-01 2021-01-14 암젠 인크 조절된 글리칸 프로파일을 갖는 항체

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
AU588819B2 (en) 1984-10-29 1989-09-28 Immunex Corporation Cloning of human granulocyte-macrophage colony stimulating factor gene
US4968607A (en) 1987-11-25 1990-11-06 Immunex Corporation Interleukin-1 receptors
US5075222A (en) 1988-05-27 1991-12-24 Synergen, Inc. Interleukin-1 inhibitors
WO1990005183A1 (en) 1988-10-31 1990-05-17 Immunex Corporation Interleukin-4 receptors
US5395760A (en) 1989-09-05 1995-03-07 Immunex Corporation DNA encoding tumor necrosis factor-α and -β receptors
ATE236249T1 (de) 1989-09-12 2003-04-15 Hoffmann La Roche Tfn-bindende proteine
US6204363B1 (en) 1989-10-16 2001-03-20 Amgen Inc. Stem cell factor
US5149792A (en) 1989-12-19 1992-09-22 Amgen Inc. Platelet-derived growth factor B chain analogs
US5272064A (en) 1989-12-19 1993-12-21 Amgen Inc. DNA molecules encoding platelet-derived growth factor B chain analogs and method for expression thereof
US5350683A (en) 1990-06-05 1994-09-27 Immunex Corporation DNA encoding type II interleukin-1 receptors
AU651596B2 (en) 1990-06-05 1994-07-28 Immunex Corporation Type II interleukin-1 receptors
AU678787B2 (en) 1992-10-23 1997-06-12 Immunex Corporation Methods of preparing soluble, oligomeric proteins
JPH06292592A (ja) * 1993-02-09 1994-10-21 Snow Brand Milk Prod Co Ltd 糖蛋白質の生産方法
US5554512A (en) 1993-05-24 1996-09-10 Immunex Corporation Ligands for flt3 receptors
US5981713A (en) 1994-10-13 1999-11-09 Applied Research Systems Ars Holding N.V. Antibodies to intereleukin-1 antagonists
US5763223A (en) 1995-06-29 1998-06-09 Immunex Corporation DNA encoding a cytokine that induces apoptosis
US6613544B1 (en) 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin
US6096728A (en) 1996-02-09 2000-08-01 Amgen Inc. Composition and method for treating inflammatory diseases
US6271349B1 (en) 1996-12-23 2001-08-07 Immunex Corporation Receptor activator of NF-κB
US6235883B1 (en) 1997-05-05 2001-05-22 Abgenix, Inc. Human monoclonal antibodies to epidermal growth factor receptor
DE59813187D1 (de) * 1997-12-03 2005-12-15 Roche Diagnostics Gmbh Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung
US6337072B1 (en) 1998-04-03 2002-01-08 Hyseq, Inc. Interleukin-1 receptor antagonist and recombinant production thereof
AU1920301A (en) 1999-11-17 2001-05-30 Immunex Corporation Receptor activator of nf-kappa b
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
SI1543106T1 (sl) 2002-07-15 2007-08-31 Immunex Corp Postopki in mediji za nadziranje sialilacije proteinov, ki jih proizvajajo sesalske celice
ES2354610T5 (es) * 2002-12-23 2020-09-14 Bristol Myers Squibb Co Mejora de la calidad del producto en procedimientos de cultivo de células de mamífero para la producción de proteína
DE102005046225B4 (de) 2005-09-28 2012-01-05 Cellca Gmbh Verbessertes Zellkulturmedium
US20070190057A1 (en) 2006-01-23 2007-08-16 Jian Wu Methods for modulating mannose content of recombinant proteins
KR20110139292A (ko) 2009-04-20 2011-12-28 화이자 인코포레이티드 단백질 글리코실화의 제어 및 그와 관련된 조성물 및 방법
BR112013009275A2 (pt) 2010-10-20 2017-06-20 Morphotek Inc glicoformas de anticorpo alfa de receptor anti-folato
US9133493B2 (en) 2011-04-21 2015-09-15 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
WO2012149197A2 (en) * 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
ES2625045T3 (es) * 2011-07-01 2017-07-18 Amgen Inc. Cultivo de células de mamífero
TWI625390B (zh) 2013-03-14 2018-06-01 安美基公司 用於增加重組蛋白質之甘露糖含量之方法
US9481901B2 (en) * 2013-05-30 2016-11-01 Amgen Inc. Methods for increasing mannose content of recombinant proteins

Also Published As

Publication number Publication date
RS64326B1 (sr) 2023-08-31
CY1120824T1 (el) 2019-12-11
PT3434760T (pt) 2022-07-05
TWI771890B (zh) 2022-07-21
PL2970874T3 (pl) 2019-03-29
US11952605B2 (en) 2024-04-09
WO2014159259A1 (en) 2014-10-02
US20200087698A1 (en) 2020-03-19
US11459595B2 (en) 2022-10-04
EP4141104A1 (en) 2023-03-01
US9822388B2 (en) 2017-11-21
TW201831677A (zh) 2018-09-01
US11319568B2 (en) 2022-05-03
DK3434760T3 (da) 2023-07-24
TWI832345B (zh) 2024-02-11
US20230193340A1 (en) 2023-06-22
EP3434760A1 (en) 2019-01-30
TW201441368A (zh) 2014-11-01
PT2970874T (pt) 2018-11-16
FI3434760T3 (fi) 2023-06-12
LT2970874T (lt) 2018-12-10
TR201815892T4 (tr) 2018-11-21
SI2970874T1 (sl) 2019-01-31
TW202311525A (zh) 2023-03-16
ES2949507T3 (es) 2023-09-29
HRP20230667T1 (hr) 2023-10-13
HUE063435T2 (hu) 2024-01-28
US20180002733A1 (en) 2018-01-04
EP3434760B1 (en) 2023-04-26
EP2970874A1 (en) 2016-01-20
ES2691801T3 (es) 2018-11-28
SI3434760T1 (sl) 2022-08-31
TW202142685A (zh) 2021-11-16
US20220213522A1 (en) 2022-07-07
EP2970874B1 (en) 2018-09-19
LT3434760T (lt) 2022-06-27
US20220136026A1 (en) 2022-05-05
TWI720289B (zh) 2021-03-01
US20160032343A1 (en) 2016-02-04
PL3434760T3 (pl) 2023-09-11
HUE040682T2 (hu) 2019-03-28
TWI625390B (zh) 2018-06-01
DK2970874T3 (en) 2018-11-19

Similar Documents

Publication Publication Date Title
AR095418A1 (es) Métodos para aumentar el contenido de manosa en proteínas recombinantes
AR124871A2 (es) Métodos de fabricación para el control del contenido de lisina c-terminal, galactosa y ácido siálico en proteínas recombinantes
CY1121217T1 (el) Μεθοδοι για συγκομιδη καλλιεργειων κυτταρου θηλαστικων
MX2022003994A (es) Produccion de oligosacaridos de leche humana en huespedes microbianos con importacion/exportacion dise?ada.
CY1125392T1 (el) Συνθεσεις και μεθοδοι γλυζυλιωσης πρωτεϊνων
EA201690529A1 (ru) Способы модификации клетки-хозяина
MX2020011334A (es) Cultivo celular metabolicamente optimizado.
WO2016022377A3 (en) Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen
EA201590488A1 (ru) Способы модификации клеток-хозяев
BR112014018630A2 (pt) alvejamento de glicanos de sulfato de condroitina
AR094375A1 (es) Métodos de uso de inhibidores del ciclo celular para modular una o más propiedades de un cultivo celular
MX2019006633A (es) Formulaciones de anticuerpo.
AR092712A1 (es) Una proteina con actividad cetosa 3-epimerasa
ES2721309T3 (es) Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa
BR112015021189A2 (pt) métodos de banco de células de alta densidade
MX2020012356A (es) Sobreexpresion de reguladores de la ruta de n-glicosilacion para modular la glicosilacion de proteinas recombinantes.
EA202091349A1 (ru) Способы культивирования клеток
EA202190736A1 (ru) Способы и композиции для увеличения экспрессии белка и/или лечения расстройства, связанного с гаплонедостаточностью
MY180183A (en) Methods and compositions for preventing norleucine misincorporation into proteins
MX2016011465A (es) Drimenol-sintasas y metodos para producir drimenol.
BR112015019719A2 (pt) Proteínas melhoradas de ligação ao tnf
BR112016015812A2 (pt) Método para aumentar a biomassa e o conteúdo lipídico em um microrganismo e um microrganismo geneticamente modificado exibindo autofagia reforçada
MX2016013955A (es) Drimenol-sintasas y metodo de produccion de drimenol.
MX2015010887A (es) Formulaciones y metodos para aumentar la produccion de proteinas recombinantes.
MX2016005572A (es) Uso de monensina para regular la glicosilacion de proteinas recombinantes.

Legal Events

Date Code Title Description
FB Suspension of granting procedure