AR095418A1 - Métodos para aumentar el contenido de manosa en proteínas recombinantes - Google Patents

Métodos para aumentar el contenido de manosa en proteínas recombinantes

Info

Publication number
AR095418A1
AR095418A1 ARP140100962A ARP140100962A AR095418A1 AR 095418 A1 AR095418 A1 AR 095418A1 AR P140100962 A ARP140100962 A AR P140100962A AR P140100962 A ARP140100962 A AR P140100962A AR 095418 A1 AR095418 A1 AR 095418A1
Authority
AR
Argentina
Prior art keywords
manose
methods
content
increase
recombinant proteins
Prior art date
Application number
ARP140100962A
Other languages
English (en)
Inventor
R Lindahl Katherine
C Brandestein Michael
Erik Lillie Shawn
P Davern Sean
Wu Jian
Crina Petrovan Slmina
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50397323&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR095418(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AR095418A1 publication Critical patent/AR095418A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Un método para modular una o más especies de glicano de alto contenido de manosa sobre una proteína recombinante durante un proceso de cultivo de células de mamífero caracterizado porque comprende limitar la cantidad de glucosa en el medio de cultivo celular y suplementar el medio de cultivo celular con galactosa o sacarosa.
ARP140100962A 2013-03-14 2014-03-13 Métodos para aumentar el contenido de manosa en proteínas recombinantes AR095418A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361784639P 2013-03-14 2013-03-14

Publications (1)

Publication Number Publication Date
AR095418A1 true AR095418A1 (es) 2015-10-14

Family

ID=50397323

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140100962A AR095418A1 (es) 2013-03-14 2014-03-13 Métodos para aumentar el contenido de manosa en proteínas recombinantes

Country Status (17)

Country Link
US (6) US9822388B2 (es)
EP (3) EP4141104A1 (es)
AR (1) AR095418A1 (es)
CY (1) CY1120824T1 (es)
DK (2) DK3434760T3 (es)
ES (2) ES2949507T3 (es)
FI (1) FI3434760T3 (es)
HR (1) HRP20230667T1 (es)
HU (2) HUE063435T2 (es)
LT (2) LT2970874T (es)
PL (2) PL3434760T3 (es)
PT (2) PT3434760T (es)
RS (1) RS64326B1 (es)
SI (2) SI2970874T1 (es)
TR (1) TR201815892T4 (es)
TW (4) TWI625390B (es)
WO (1) WO2014159259A1 (es)

Families Citing this family (10)

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FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
PL2726600T3 (pl) 2011-07-01 2017-08-31 Amgen Inc. Hodowla ssaczych komórek
WO2013114245A1 (en) 2012-01-30 2013-08-08 Dr. Reddy's Laboratories Limited Process of modulating man5 and/or afucosylation content of glycoprotein composition
TWI625390B (zh) 2013-03-14 2018-06-01 安美基公司 用於增加重組蛋白質之甘露糖含量之方法
MX2016005572A (es) * 2013-10-31 2016-12-09 Amgen Inc Uso de monensina para regular la glicosilacion de proteinas recombinantes.
EP3390438A1 (en) * 2015-12-15 2018-10-24 CSL Behring Recombinant Facility AG Method for increasing the heterogeneity of o-glycosylation of recombinant factor vii
EP3585880A1 (en) 2017-02-27 2020-01-01 Life Technologies Corporation Expansion of populations of t cells by the use of modified serum free media
WO2019094333A1 (en) * 2017-11-13 2019-05-16 Amgen Inc. Methods for producing protein products
JP7267284B2 (ja) 2017-12-20 2023-05-01 アレス トレーディング ソシエテ アノニム ポリエーテルイオノフォアを使用するタンパク質マンノシル化プロファイルの調節方法
KR20210005689A (ko) * 2018-05-01 2021-01-14 암젠 인크 조절된 글리칸 프로파일을 갖는 항체

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WO2012149197A2 (en) * 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
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Also Published As

Publication number Publication date
US11319568B2 (en) 2022-05-03
TWI771890B (zh) 2022-07-21
PT2970874T (pt) 2018-11-16
EP2970874B1 (en) 2018-09-19
US11952605B2 (en) 2024-04-09
WO2014159259A1 (en) 2014-10-02
TWI720289B (zh) 2021-03-01
TWI832345B (zh) 2024-02-11
HUE063435T2 (hu) 2024-01-28
US9822388B2 (en) 2017-11-21
SI2970874T1 (sl) 2019-01-31
TW202311525A (zh) 2023-03-16
RS64326B1 (sr) 2023-08-31
SI3434760T1 (sl) 2022-08-31
US20220213522A1 (en) 2022-07-07
US20220136026A1 (en) 2022-05-05
PL2970874T3 (pl) 2019-03-29
US20230193340A1 (en) 2023-06-22
EP2970874A1 (en) 2016-01-20
EP4141104A1 (en) 2023-03-01
DK3434760T3 (da) 2023-07-24
LT3434760T (lt) 2022-06-27
EP3434760A1 (en) 2019-01-30
ES2691801T3 (es) 2018-11-28
US20200087698A1 (en) 2020-03-19
HRP20230667T1 (hr) 2023-10-13
CY1120824T1 (el) 2019-12-11
HUE040682T2 (hu) 2019-03-28
TW202142685A (zh) 2021-11-16
ES2949507T3 (es) 2023-09-29
US20180002733A1 (en) 2018-01-04
TW201831677A (zh) 2018-09-01
US20160032343A1 (en) 2016-02-04
FI3434760T3 (fi) 2023-06-12
EP3434760B1 (en) 2023-04-26
PL3434760T3 (pl) 2023-09-11
PT3434760T (pt) 2022-07-05
TWI625390B (zh) 2018-06-01
DK2970874T3 (en) 2018-11-19
TW201441368A (zh) 2014-11-01
LT2970874T (lt) 2018-12-10
TR201815892T4 (tr) 2018-11-21
US11459595B2 (en) 2022-10-04

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