AR087579A1 - Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta - Google Patents

Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta

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Publication number
AR087579A1
AR087579A1 ARP120103024A ARP120103024A AR087579A1 AR 087579 A1 AR087579 A1 AR 087579A1 AR P120103024 A ARP120103024 A AR P120103024A AR P120103024 A ARP120103024 A AR P120103024A AR 087579 A1 AR087579 A1 AR 087579A1
Authority
AR
Argentina
Prior art keywords
plant
amino acid
seq
domain
pyruvate kinase
Prior art date
Application number
ARP120103024A
Other languages
English (en)
Original Assignee
Basf Plant Science Co Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Co Gmbh filed Critical Basf Plant Science Co Gmbh
Publication of AR087579A1 publication Critical patent/AR087579A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fats And Perfumes (AREA)

Abstract

Métodos para preparar una planta, célula vegetal o parte de planta con un mayor contenido de una o más proteínas, aceites o uno o más aminoácidos con respecto a una planta de tipo silvestre, célula vegetal o parte de planta correspondiente. También casetes de expresión para lograr la manipulación de la expresión génica, así como constructos recombinantes, vectores y plantas, células vegetales o partes de plantas que los comprenden. Las plantas, células vegetales o partes de plantas con un mayor contenido de una o más proteínas, aceites o uno o más aminoácidos obtenidos de esa manera pueden ser útiles para preparar productos alimenticios para seres humanos y para animales. Las plantas, células vegetales o partes de plantas con un mayor contenido de una o más proteínas, aceites o uno o más aminoácidos obtenidos de esa manera también pueden ser útiles en programas de reproducción de plantas para desarrollar otras líneas híbridas o endogámicas. Reivindicación 1: Un casete de expresión que confiere un mayor contenido de una o más proteínas, aceites o uno o más aminoácidos en una planta, célula vegetal o parte de planta con respecto a una planta de tipo silvestre, célula vegetal o parte de planta correspondiente, caracterizado porque comprende: (a) un promotor que es funcional en una planta; (b) una molécula de ácido nucleico que codifica un polipéptido con actividad de piruvato quinasa que es heterólogo y está operativamente ligado a dicho promotor; y (c) un intrón del arroz, en donde la molécula de ácido nucleico comprende: (i) la secuencia de nucleótidos de SEQ ID Nº 1, 3, 5, 7, 9, 11 ó 13; (ii) una secuencia de nucleótidos que codifica la secuencia de aminoácidos de SEQ ID Nº 2, 4, 6, 8, 10, 12 ó 14; (iii) una secuencia de nucleótidos que tiene al menos 60% de identidad con la secuencia de nucleótidos de SEQ ID Nº 1, 3, 5, 7, 9, 11 ó 13 y que codifica un polipéptido que tiene un dominio barril de piruvato quinasa Pfam:PF00224 y un dominio a/b de piruvato quinasa Pfam:PF02887; (iv) una secuencia de nucleótidos que codifica una secuencia de aminoácidos que tiene al menos 60% de identidad con la secuencia de aminoácidos de SEQ ID Nº 2, 4, 6, 8, 10, 12 ó 14 y que tiene un dominio barril de piruvato quinasa Pfam:PF00224 y un dominio a/b de piruvato quinasa Pfam:PF02887; (v) una secuencia de nucleótidos que codifica una secuencia de aminoácidos que comprende un dominio barril de piruvato quinasa Pfam:PF00224 y un dominio a/b de piruvato quinasa Pfam:PF02887, en donde el dominio barril de piruvato quinasa Pfam:PF00224 tiene al menos 80% de identidad con los residuos de aminoácidos 109 a 449 de SEQ ID Nº 2 o los residuos de aminoácidos 98 a 439 de SEQ ID Nº 10, y en donde el dominio a/b de piruvato quinasa Pfam:PF02887 tiene al menos 80% de identidad con los residuos de aminoácidos 462 a 578 de SEQ ID Nº 2 o los residuos de aminoácidos 452 a 566 de SEQ ID Nº 10; ó (vi) una secuencia de nucleótidos que codifica una secuencia de aminoácidos que tiene al menos 60% de identidad con la secuencia de aminoácidos de SEQ ID Nº 2, 4, 6, 8, 10, 12 ó 14, en donde la secuencia de aminoácidos también comprende la secuencia de aminoácidos de SEQ ID Nº 102 y 103.
ARP120103024A 2011-08-19 2012-08-16 Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta AR087579A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161525232P 2011-08-19 2011-08-19

Publications (1)

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AR087579A1 true AR087579A1 (es) 2014-04-03

Family

ID=47712840

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120103024A AR087579A1 (es) 2011-08-19 2012-08-16 Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta

Country Status (4)

Country Link
US (1) US20130045325A1 (es)
AR (1) AR087579A1 (es)
UY (1) UY34266A (es)
WO (1) WO2013027159A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105087589B (zh) * 2015-09-21 2017-11-03 安徽省农业科学院水稻研究所 一种启动子元件OsEmb2及利用其培育转基因水稻的方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
AU2006249820A1 (en) * 2005-05-26 2006-11-30 Monsanto Technology Llc Elevation of oil in monocot plants

Also Published As

Publication number Publication date
UY34266A (es) 2013-02-28
WO2013027159A1 (en) 2013-02-28
US20130045325A1 (en) 2013-02-21

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