AR065685A1 - Ensayo basado en fluorescencia para detectar compuestos moduladores del modo directo del intercambiador sodio- calcio (ncx) - Google Patents

Ensayo basado en fluorescencia para detectar compuestos moduladores del modo directo del intercambiador sodio- calcio (ncx)

Info

Publication number
AR065685A1
AR065685A1 ARP080100992A ARP080100992A AR065685A1 AR 065685 A1 AR065685 A1 AR 065685A1 AR P080100992 A ARP080100992 A AR P080100992A AR P080100992 A ARP080100992 A AR P080100992A AR 065685 A1 AR065685 A1 AR 065685A1
Authority
AR
Argentina
Prior art keywords
ncx
cells
activity
calcium
sodium
Prior art date
Application number
ARP080100992A
Other languages
English (en)
Original Assignee
Anesini Claudia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anesini Claudia filed Critical Anesini Claudia
Publication of AR065685A1 publication Critical patent/AR065685A1/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Los transportadores son una familia diana emergente con un potencial enorme, que ofrece oportunidades científicas y economicas. El intercambiador sodio/calcio es un mecanismo importante para eliminar el Ca2+ de diversas células. En el corazon,extruye el Ca2+ que ha ingresado a través de los canales de Ca2+ para iniciar la contraccion mientras que el Na+ ingresa a las células del corazon. Resulta de considerable interés identificar compuestos que modulen la actividad de losintercambiadores sodio/calcio. Se divulga un ensayo basado en fluorescencia para detectar compuestos moduladores del ômodo directoö del NCX. También se refiere a un kit de partes que comprende células que exprimen el NCX y al uso del kit de partespara ensayar la actividad como agonista o antagonista del NCX en un compuesto. Reivindicacion 1: El ensayo para determinar la actividad de la proteína del NCX, comprende: e) proveer células que expresan NCX; f) proveer una sustancia coloreadapara determinar el calcio intracelular; g) poner en contacto las células con un activador de la actividad del NCX; y h) comparar el cambio mediado por calcio en la senal luminiscente de dicha sustancia coloreada con una senal luminiscente producidaen un experimento de control.
ARP080100992A 2007-03-13 2008-03-11 Ensayo basado en fluorescencia para detectar compuestos moduladores del modo directo del intercambiador sodio- calcio (ncx) AR065685A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102007011913A DE102007011913A1 (de) 2007-03-13 2007-03-13 Fluoreszenz-basiertes Assay zum Erkennen von Verbindungen zum Modulieren des Natrium-Calcium-Austauschers (NCX) im "Vorwärtsmodus"

Publications (1)

Publication Number Publication Date
AR065685A1 true AR065685A1 (es) 2009-06-24

Family

ID=39563393

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080100992A AR065685A1 (es) 2007-03-13 2008-03-11 Ensayo basado en fluorescencia para detectar compuestos moduladores del modo directo del intercambiador sodio- calcio (ncx)

Country Status (14)

Country Link
US (1) US20100151497A1 (es)
EP (1) EP2135092A1 (es)
JP (1) JP2010520759A (es)
KR (1) KR20090122287A (es)
CN (1) CN101636658A (es)
AR (1) AR065685A1 (es)
AU (1) AU2008226101B2 (es)
BR (1) BRPI0809076A2 (es)
CA (1) CA2680768A1 (es)
DE (1) DE102007011913A1 (es)
IL (1) IL200789A0 (es)
MX (1) MX2009009681A (es)
TW (1) TW200907340A (es)
WO (1) WO2008110285A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2103944A1 (en) * 2008-03-20 2009-09-23 sanofi-aventis Fluorescence based assay to detect sodium/calcium exchanger "forward mode" modulating compounds
JP5127783B2 (ja) * 2009-06-29 2013-01-23 日本電信電話株式会社 においセンサおよびにおい検知方法
US8568471B2 (en) * 2010-01-30 2013-10-29 Abbott Cardiovascular Systems Inc. Crush recoverable polymer scaffolds
US8808353B2 (en) * 2010-01-30 2014-08-19 Abbott Cardiovascular Systems Inc. Crush recoverable polymer scaffolds having a low crossing profile

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714666A (en) 1993-02-09 1998-02-03 Children's Hospital Of Philadelphia Measurement of intracellular calcium using bioluminescent apoaequorin expressed in mammalian cells
US6162832A (en) 1997-10-20 2000-12-19 Taisho Pharmaceutical Co., Inc. 2-Phenoxyaniline derivatives
US20020132303A1 (en) * 2001-03-12 2002-09-19 Millennium Pharmaceuticals, Inc. 69318, a human sodium/calcium exchanger (transporter) family member and uses therefor
AU2002215350B2 (en) * 2000-10-13 2006-12-07 Bristol-Myers Squibb Company Methods for detecting modulators of ion channels using thallium (I) sensitive assays
KR20040062623A (ko) * 2001-11-09 2004-07-07 화이자 프로덕츠 인크. 작용제에 의한 수용체의 활성화에 대한 기능 분석법
KR100514090B1 (ko) * 2003-01-17 2005-09-13 한국과학기술연구원 Ｎｃｘ2 단백질의 활성을 억제함으로써 학습능력 및기억력을 증진시키는 방법
US20050112701A1 (en) * 2003-09-11 2005-05-26 Aventis Pharma Deutschland Gmbh Test system for the identification of APJ receptor ligands

Also Published As

Publication number Publication date
AU2008226101B2 (en) 2013-09-19
EP2135092A1 (de) 2009-12-23
AU2008226101A1 (en) 2008-09-18
WO2008110285A1 (de) 2008-09-18
CA2680768A1 (en) 2008-09-18
BRPI0809076A2 (pt) 2014-11-04
MX2009009681A (es) 2009-09-24
US20100151497A1 (en) 2010-06-17
JP2010520759A (ja) 2010-06-17
IL200789A0 (en) 2010-05-17
TW200907340A (en) 2009-02-16
KR20090122287A (ko) 2009-11-26
DE102007011913A1 (de) 2008-10-23
CN101636658A (zh) 2010-01-27

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