AR065350A1 - Moleculas de acido nucleicos que codifican una alternanosucrasa truncada - Google Patents

Moleculas de acido nucleicos que codifican una alternanosucrasa truncada

Info

Publication number
AR065350A1
AR065350A1 ARP080100633A ARP080100633A AR065350A1 AR 065350 A1 AR065350 A1 AR 065350A1 AR P080100633 A ARP080100633 A AR P080100633A AR P080100633 A ARP080100633 A AR P080100633A AR 065350 A1 AR065350 A1 AR 065350A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
sequence
seq
acid molecule
amino acid
Prior art date
Application number
ARP080100633A
Other languages
English (en)
Original Assignee
Bayer Cropscience Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Cropscience Ag filed Critical Bayer Cropscience Ag
Publication of AR065350A1 publication Critical patent/AR065350A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/0114—Alternansucrase (2.4.1.140)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicinal Preparation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Moléculas de ácidos nucleicos, que codifican a una alternanosucrasa truncada, leuconostoc mesenteroides. Además a vectores, células huésped así como también células de plantas transformadas con las moléculas de ácidos nucleicos descriptas y lasplantas que contienen estas células. Se refiere además a polímeros de alternano con propiedades ventajosas y a procedimientos para su preparacion. Reivindicacion 1: Molécula de ácido nucleico, que codifica una proteína con la actividad enzimáticade una alternanosucrasa, en donde la molécula de ácido nucleico es seleccionada entre: a) una molécula de ácido nucleico con una secuencia del nucleotido en la posicion 118 al nucleotido en la posicion 4140 inclusive, de la secuencia de ácidosnucleicos representada bajo SEQ ID N°:1 o SEQ ID N°: 3, b) una molécula de ácido nucleico, que codifica una proteína, cuya secuencia de aminoácidos presenta una identidad de por lo menos 70% con la secuencia del aminoácido en la posicion 40 alaminoácido en la posicion 1380 inclusive, de la SEQ ID N°:2, c) una molécula de ácido nucleico con una secuencia del nucleotido en la posicion 118 al nucleotido en la posicion 3945 inclusive, de la secuencia de ácidos nucleicos representada bajo SEQID N°:1 o SEQ ID N°:3, d) una molécula de ácido nucleico, que codifica una proteína, cuya secuencia de aminoácidos presenta una identidad de por lo menos 70% con la secuencia del aminoácido en la posicion 40 al aminoácido en la posicion 1315inclusive, de la SEQ ID N°:2, e) una molécula de ácido nucleico con una secuencia del nucleotido en la posicion 118 al nucleotido en la posicion 4518 inclusive, de la secuencia de ácidos nucleicos representada bajo la SEQ ID N°: 1 o la SEQ ID N° 3,f) una molécula de ácido nucleico, que codifica una proteína, cuya secuencia de aminoácidos presenta una identidad de por lo menos 70% con la secuencia del aminoácido en la posicion 40 al aminoácido en la posicion 1506 inclusive de la SEQ ID N°: 2,g) una molécula de ácido nucleico, cuya secuencia presenta una identidad de por lo menos 70% con la secuencia de a), c) o e), donde dicha secuencia tiene el mismo numero de nucleotidos como la secuencia de a), c) o e), h) una molécula de ácidonucleico, cuya secuencia de ácidos nucleicos se diferencia de la secuencia de la molécula de ácido nucleico de a), b), c), d), e), f) o g), debido a la degeneracion del codigo genético; i) una molécula de ácido nucleico, cuya secuencia de ácidosnucleicos es complementaria en longitud completa con respecto a la secuencia de una de las moléculas de ácidos nucleicos de a), b), c), d), e), f), g) o h), donde la secuencia complementaria tiene la misma cantidad de nucleotidos que las moléculasde ácido nucleico de a), b), c), d), e), f), g) o h).
ARP080100633A 2007-02-14 2008-02-14 Moleculas de acido nucleicos que codifican una alternanosucrasa truncada AR065350A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP07090022 2007-02-14

Publications (1)

Publication Number Publication Date
AR065350A1 true AR065350A1 (es) 2009-06-03

Family

ID=38055540

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080100633A AR065350A1 (es) 2007-02-14 2008-02-14 Moleculas de acido nucleicos que codifican una alternanosucrasa truncada

Country Status (11)

Country Link
US (1) US9365861B2 (es)
EP (1) EP2121908B1 (es)
JP (1) JP5405321B2 (es)
CN (1) CN101611140B (es)
AR (1) AR065350A1 (es)
AU (1) AU2008214612B2 (es)
CA (1) CA2677469C (es)
DK (1) DK2121908T3 (es)
ES (1) ES2438001T3 (es)
WO (1) WO2008098975A1 (es)
ZA (1) ZA200904559B (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2098123A1 (de) 2008-03-07 2009-09-09 Bayer CropScience AG Verwendung von Alternan als Verdickungsmittel und Verdickungsmittelzusammensetzungen enthaltend Alternan und ein weiteres Verdickungsmittel.
EP2100517A1 (en) 2008-03-07 2009-09-16 Bayer CropScience Aktiengesellschaft Use of alternan as texturizing agent in foodstuffs and cosmetics
AU2013202754B2 (en) * 2008-03-07 2014-09-11 Bayer Cropscience Aktiengesellschaft Use of alternan as texturizing agent in foodstuffs and cosmetics
EP2239331A1 (en) 2009-04-07 2010-10-13 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
CN102492673B (zh) * 2011-12-28 2013-01-23 江南大学 一种柠檬明串珠菌发酵生产交替糖蔗糖酶的方法及应用
EP2700656A1 (de) 2012-08-24 2014-02-26 aevotis GmbH Carboxy-funktionalisiertes Alternan
EP2700657A1 (de) 2012-08-24 2014-02-26 aevotis GmbH Alternan-Polysaccharid, das mit protonierbaren Stickstoffgruppen oder permanent positiv geladenen Stickstoffgruppen funktionalisiert ist
US20170006902A1 (en) * 2014-02-13 2017-01-12 Danisco Us Inc. Strategy for sucrose reduction and generation of insoluble fiber in juices
MX2016015612A (es) 2014-05-29 2017-03-13 Du Pont Síntesis enzimática de fibra soluble de glucano.
EP3149184A1 (en) 2014-05-29 2017-04-05 E. I. du Pont de Nemours and Company Enzymatic synthesis of soluble glucan fiber
MX2016015605A (es) 2014-05-29 2017-03-10 Du Pont Síntesis enzimática de fibra soluble de glucano.
BR112016027839A2 (pt) 2014-05-29 2017-10-31 Du Pont composições de fibra e de carboidrato, produto alimentício, métodos de produção de composição de fibra, de elaboração de composição de carboidrato misturada, de redução do índice glicêmico de alimentos ou bebidas e de inibição da elevação do nível de açúcar no sangue, composição cosmética, uso da composição de fibra e produto
WO2015183714A1 (en) 2014-05-29 2015-12-03 E. I. Du Pont De Nemours And Company Enzymatic synthesis of soluble glucan fiber
CN107580453A (zh) 2014-05-29 2018-01-12 纳幕尔杜邦公司 可溶性葡聚糖纤维的酶促合成
CN107205910A (zh) * 2015-02-06 2017-09-26 纳幕尔杜邦公司 基于聚α‑1,3‑葡聚糖的聚合物的胶体分散体
EP3374400B1 (en) 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
JP7064435B2 (ja) 2015-11-26 2022-05-10 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド α－１，２－分岐鎖を有するグルカンを生成することができるポリペプチドおよびその使用
BR112021001800A2 (pt) * 2018-07-31 2021-05-04 Bayer Aktiengesellschaft ácidos nucleicos que codificam proteínas transaminase aprimoradas

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19905069A1 (de) * 1999-02-08 2000-08-10 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle codierend Alternansucrase
US6479275B1 (en) * 2001-07-25 2002-11-12 The United States Of America As Represented By The Secretary Of Agriculture Penicillium isolates for modifying alternan
GB0221184D0 (en) 2002-09-13 2002-10-23 Cerestar Holding Bv Carbohydrate materials in food and drink compositions
US7524645B2 (en) * 2004-12-14 2009-04-28 Centre National De La Recherche Scientifique (Cnrs) Fully active alternansucrases partially deleted in its carboxy-terminal and amino-terminal domains and mutants thereof

Also Published As

Publication number Publication date
EP2121908A1 (en) 2009-11-25
AU2008214612A1 (en) 2008-08-21
CA2677469C (en) 2016-08-16
CN101611140B (zh) 2013-01-02
WO2008098975A1 (en) 2008-08-21
AU2008214612B2 (en) 2013-12-19
ZA200904559B (en) 2010-09-29
US9365861B2 (en) 2016-06-14
ES2438001T3 (es) 2014-01-15
CA2677469A1 (en) 2008-08-21
JP5405321B2 (ja) 2014-02-05
EP2121908B1 (en) 2013-10-23
CN101611140A (zh) 2009-12-23
DK2121908T3 (da) 2014-01-27
US20100122378A1 (en) 2010-05-13
JP2010517588A (ja) 2010-05-27

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