AR064703A1 - Lineas celulares inmortales diferenciadas capaces de producir albumina y factores de coagulacion , procedimientos para su preparacion a partir de una linea celular leucemica y sus empleos - Google Patents

Lineas celulares inmortales diferenciadas capaces de producir albumina y factores de coagulacion , procedimientos para su preparacion a partir de una linea celular leucemica y sus empleos

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Publication number
AR064703A1
AR064703A1 ARP070105978A ARP070105978A AR064703A1 AR 064703 A1 AR064703 A1 AR 064703A1 AR P070105978 A ARP070105978 A AR P070105978A AR P070105978 A ARP070105978 A AR P070105978A AR 064703 A1 AR064703 A1 AR 064703A1
Authority
AR
Argentina
Prior art keywords
cell line
coagulation factors
procedures
thp1
differentiated
Prior art date
Application number
ARP070105978A
Other languages
English (en)
Inventor
Antonio Ponzetto
Giampiero Pescarmona
Andrea Savarino
Luisa Gennero
Gianfranco Merizzi
Original Assignee
Medestea Biotech Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medestea Biotech Srl filed Critical Medestea Biotech Srl
Publication of AR064703A1 publication Critical patent/AR064703A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/805—Acetylcholine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oncology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente solicitud tiene por objeto las líneas celulares diferenciadas que tienen fenotipo hepatocitario capaz de producir albumina y factores de la coagulacion, siendo tales células derivadas de una línea celular humana leucémica, preferiblemente la línea celular humana THP1, y conservando las características de inmortalidad. Entre las líneas celulares de la presente, son preferidas las líneas denominadas PSC-THP1-EP, PSC-THP1-EP-FAST, PSC-THP1-HEP e PSC-THP1-EPEP. La presente es también relativa a los procedimientos para la obtencion de las líneas celulares de la presente, y de los empleos de tales líneas celulares en particular para la produccion de albumina y/o factores de coagulacion
ARP070105978A 2006-12-29 2007-12-28 Lineas celulares inmortales diferenciadas capaces de producir albumina y factores de coagulacion , procedimientos para su preparacion a partir de una linea celular leucemica y sus empleos AR064703A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT000937A ITTO20060937A1 (it) 2006-12-29 2006-12-29 Linee cellulari immortali differenziate atte a produrre albumina e fattori di crescita, procedimenti per la loro preparazione da una linea cellulare leucemica e loro usi.

Publications (1)

Publication Number Publication Date
AR064703A1 true AR064703A1 (es) 2009-04-22

Family

ID=39332214

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP070105978A AR064703A1 (es) 2006-12-29 2007-12-28 Lineas celulares inmortales diferenciadas capaces de producir albumina y factores de coagulacion , procedimientos para su preparacion a partir de una linea celular leucemica y sus empleos

Country Status (8)

Country Link
US (1) US20110045519A1 (es)
EP (1) EP2102329B1 (es)
CN (1) CN101679945A (es)
AR (1) AR064703A1 (es)
AT (1) ATE469210T1 (es)
DE (1) DE602007006844D1 (es)
IT (1) ITTO20060937A1 (es)
WO (1) WO2008081388A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120295344A1 (en) * 2006-08-25 2012-11-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Stem Cell Fusion Model of Carcinogenesis
CN101851604B (zh) * 2010-04-20 2012-04-18 熊军 一种人血白蛋白的生产方法
CN107523537A (zh) * 2017-10-23 2017-12-29 洪丰 一种成体人肝源性干细胞系hn及其制备方法和应用
CN108330107B (zh) * 2018-04-24 2021-10-29 富恩生物技术（成都）有限公司 杂交瘤细胞株、cd68单克隆抗体、制备方法及应用
CN108753725A (zh) * 2018-05-23 2018-11-06 浙江大学 人血白蛋白高表达的人肝细胞系及其建立方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2089213T3 (es) * 1990-05-16 1996-10-01 Baylor College Medicine Una linea celular permanente de hepatocitos humanos y su uso en un dispositivo de asistencia hepatica.
TWI288779B (en) * 2002-03-28 2007-10-21 Blasticon Biotech Forschung Dedifferentiated, programmable stem cells of monocytic origin, and their production and use
ITTO20050819A1 (it) * 2005-11-18 2007-05-19 Univ Degli Studi Torino Linea cellulare staminale pluripotente, linee cellulari derivate, procedimenti per la loro produzione e relativi usi

Also Published As

Publication number Publication date
WO2008081388A8 (en) 2008-09-25
EP2102329A1 (en) 2009-09-23
ATE469210T1 (de) 2010-06-15
US20110045519A1 (en) 2011-02-24
WO2008081388A1 (en) 2008-07-10
DE602007006844D1 (de) 2010-07-08
EP2102329B1 (en) 2010-05-26
CN101679945A (zh) 2010-03-24
ITTO20060937A1 (it) 2008-06-30

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