AR035696A1 - Proteinas cry modificadas, sensibles a la pepsina; proceso para incrementar la sensibilidad de las proteinas cry a la pepsina, polinucleotidos que codifican dichas proteinas modificadas; genes quimericos que incluyen dichos polinucleotidos y vectores de expresion o de transformacion que comprenden l - Google Patents
Proteinas cry modificadas, sensibles a la pepsina; proceso para incrementar la sensibilidad de las proteinas cry a la pepsina, polinucleotidos que codifican dichas proteinas modificadas; genes quimericos que incluyen dichos polinucleotidos y vectores de expresion o de transformacion que comprenden lInfo
- Publication number
- AR035696A1 AR035696A1 ARP020100961A ARP020100961A AR035696A1 AR 035696 A1 AR035696 A1 AR 035696A1 AR P020100961 A ARP020100961 A AR P020100961A AR P020100961 A ARP020100961 A AR P020100961A AR 035696 A1 AR035696 A1 AR 035696A1
- Authority
- AR
- Argentina
- Prior art keywords
- pepsin
- cry
- modified
- proteins
- residues
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 9
- 102000004169 proteins and genes Human genes 0.000 title abstract 8
- 102000035118 modified proteins Human genes 0.000 title 2
- 108091005573 modified proteins Proteins 0.000 title 2
- 230000035945 sensitivity Effects 0.000 title 1
- 239000000126 substance Substances 0.000 title 1
- 230000009466 transformation Effects 0.000 title 1
- 239000013598 vector Substances 0.000 title 1
- 235000018102 proteins Nutrition 0.000 abstract 7
- 102000057297 Pepsin A Human genes 0.000 abstract 4
- 108090000284 Pepsin A Proteins 0.000 abstract 4
- 229940111202 pepsin Drugs 0.000 abstract 4
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 abstract 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 241000124008 Mammalia Species 0.000 abstract 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 abstract 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 108091005601 modified peptides Proteins 0.000 abstract 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 abstract 1
- 239000004474 valine Substances 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se describen y reivindican proteínas del Bacillus thuringiensis Cry, de secuencia peptídica modificada de modo de sensibilizarlos a enzimas específicas en el tracto digestivo de los mamíferos, particularmente a las pepsinas. Conforme a lo que aquí se reivindica, las proteínas Cry se modifican mediante la inserción de sitios de separación de la pepsina, en las secuencias peptídicas. Las modificaciones preferidas se representan mediante la sustitución de restos del ácido aspártico con restos del ácido glutámico, la sustitución de restos de triptófano con restos de fenilalanina y la sustitución de restos de valina o de isoleucina con restos de leucina. Una proteína Cry preferida es la proteína Cry9C, y un sitio preferido para introducir el sitio de separación de la pepsina en esta proteína es la posición 164. Además se reivindican los vegetales transformados que expresan dichas proteínas Cry modificadas y los objetos restantes mencionados en el título. Reivindicación 2: Proteína Cry modificada según la reivindicación 1, caracterizada porque el sitio adicional de corte por pepsina está representado por un resto de aminoácido elegido entre los restos leucina, fenilalanina o ácido glutámico. Reivindicación 6: Proteína Cry modificada según cualquiera de las reivindicaciones 1 a 5, caracterizada porque posee al menos un sitio adicional de corte por pepsina en al menos uno de los bucles inter-hélices alfa del dominio I.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0103691A FR2822157B1 (fr) | 2001-03-19 | 2001-03-19 | Toxine insecticide de bacillus thuringiensis modifiee sensible a la pepsine |
Publications (1)
Publication Number | Publication Date |
---|---|
AR035696A1 true AR035696A1 (es) | 2004-06-23 |
Family
ID=8861282
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP020100961A AR035696A1 (es) | 2001-03-19 | 2002-03-18 | Proteinas cry modificadas, sensibles a la pepsina; proceso para incrementar la sensibilidad de las proteinas cry a la pepsina, polinucleotidos que codifican dichas proteinas modificadas; genes quimericos que incluyen dichos polinucleotidos y vectores de expresion o de transformacion que comprenden l |
Country Status (9)
Country | Link |
---|---|
US (1) | US20040096934A1 (es) |
EP (1) | EP1370660A2 (es) |
CN (1) | CN1610744A (es) |
AR (1) | AR035696A1 (es) |
AU (1) | AU2002249311A1 (es) |
BR (1) | BR0208619A (es) |
FR (1) | FR2822157B1 (es) |
MX (1) | MXPA03008438A (es) |
WO (1) | WO2002074799A2 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2804953C (en) | 2003-12-23 | 2016-02-23 | Pioneer Hi-Bred International, Inc. | Plant activation of insect toxin |
CN101300268A (zh) | 2005-07-08 | 2008-11-05 | 墨西哥国立自治大学 | 新的具有杀虫活性的细菌蛋白质 |
WO2008094204A2 (en) * | 2006-08-02 | 2008-08-07 | John Cuppoletti | Methods for ionophorically screening pore forming bacterial protein toxins and receptors |
ES2647767T3 (es) * | 2007-12-03 | 2017-12-26 | Syngenta Participations Ag | Generación por ingeniería genética de proteínas enzimáticamente susceptibles |
CN105002189A (zh) * | 2008-06-25 | 2015-10-28 | 阿森尼克斯公司 | 毒素基因及其使用方法 |
NZ596660A (en) * | 2009-06-16 | 2013-10-25 | Dow Agrosciences Llc | Dig-5 insecticidal cry toxins |
NO344051B1 (en) * | 2017-05-04 | 2019-08-26 | Patogen As | Novel virus in Fish and Method for detection |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2160131C (en) * | 1993-04-09 | 2000-07-25 | Bart Lambert | New bacillus thuringiensis strains and their insecticidal proteins |
ES2273447T3 (es) * | 1997-12-18 | 2007-05-01 | Monsanto Technology Llc | Plantas transgenicas resistentes a insectos y procedimientos para mejorar la actividad frente a insectos. |
-
2001
- 2001-03-19 FR FR0103691A patent/FR2822157B1/fr not_active Expired - Fee Related
-
2002
- 2002-03-04 CN CNA028102053A patent/CN1610744A/zh active Pending
- 2002-03-04 MX MXPA03008438A patent/MXPA03008438A/es unknown
- 2002-03-04 BR BR0208619-0A patent/BR0208619A/pt not_active IP Right Cessation
- 2002-03-04 EP EP02718239A patent/EP1370660A2/fr not_active Withdrawn
- 2002-03-04 WO PCT/FR2002/000772 patent/WO2002074799A2/fr not_active Application Discontinuation
- 2002-03-04 AU AU2002249311A patent/AU2002249311A1/en not_active Abandoned
- 2002-03-18 AR ARP020100961A patent/AR035696A1/es not_active Application Discontinuation
-
2003
- 2003-09-19 US US10/665,460 patent/US20040096934A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1370660A2 (fr) | 2003-12-17 |
MXPA03008438A (es) | 2005-07-01 |
BR0208619A (pt) | 2004-03-30 |
FR2822157B1 (fr) | 2003-10-31 |
WO2002074799A2 (fr) | 2002-09-26 |
US20040096934A1 (en) | 2004-05-20 |
FR2822157A1 (fr) | 2002-09-20 |
WO2002074799A3 (fr) | 2003-05-01 |
CN1610744A (zh) | 2005-04-27 |
AU2002249311A1 (en) | 2002-10-03 |
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Legal Events
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FA | Abandonment or withdrawal |