AR031169A1 - 11 alfa-hidroxilasa y oxidorreductasa de aspergillus ochraceus - Google Patents

11 alfa-hidroxilasa y oxidorreductasa de aspergillus ochraceus

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Publication number
AR031169A1
AR031169A1 ARP010105071A ARP010105071A AR031169A1 AR 031169 A1 AR031169 A1 AR 031169A1 AR P010105071 A ARP010105071 A AR P010105071A AR P010105071 A ARP010105071 A AR P010105071A AR 031169 A1 AR031169 A1 AR 031169A1
Authority
AR
Argentina
Prior art keywords
alpha
oxidoreductase
aspergillus
hydroxylase
aspergillus ochraceus
Prior art date
Application number
ARP010105071A
Other languages
English (en)
Inventor
Suzanne Bolten
Alan Easton
Leslie Engel
Dean Messing
John Ng
Beverly Reitz
Mark Walker
Lijuan Jane Wang
Robert Clayton
Original Assignee
Searle Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle Llc filed Critical Searle Llc
Publication of AR031169A1 publication Critical patent/AR031169A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N3/00—Spore forming or isolating processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C12P33/10—Hydroxylating at 11 position at 11 alpha-position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Rheumatology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Cardiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pain & Pain Management (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente se relaciona con una nueva enzima relacionada con el citocromo P450 (11 alfa hidroxilasa de Aspergillus ochraceus) y con una oxidorreductasa (oxidorreductasa de Aspergillus ochraceus) aislada a partir de una biblioteca de ADNc generada a partir del ARNm de esporas de Aspergillus ochraceus. Cuando se coexpreso el ADNc que codificaba la 11 alfa hidroxilasa en células de Spodoptera frugiperda (Sf-9) con el ADNc que codificaba oxidorreductasa humana como donante de electrones, catalizo exitosamente la conversion del sustrato esteroide 4-androsten-3,17-diona (AD) en 11 alfa-hidroxi-AD, determinado por un análisis de HPLC. La presente se relaciona también con moléculas de ácidos nucleicos asociadas o derivadas de estos ADNc que incluyen complementos, homologos y fragmentos de los mismos, y métodos para usar estás moléculas de ácidos nucleicos para generar, por ejemplo, polipéptidos y fragmentos de los mismos. La presente se relaciona también con la generacion de anticuerpos que reconocen la 11 alfa hidroxilasa y la oxidorreductasa de Aspergillus Ochraceus, y métodos para usar estos anticuerpos para detectar la presencia de estos polipétidos nativos y recombinantes dentro de células huésped sin modificar y transformadas , respectivamente. La presente también provee métodos para expresar el gen de Aspergillus de 11 alfa hidroxilasa por separado o en combinacion con oxidorreductasa humana o de Aspergillus, en células huésped heterologas, para facilitar la bioconversion de sustratos esteroides en sus contrapartes de 11 alfa hidroxilo.
ARP010105071A 2000-10-30 2001-10-30 11 alfa-hidroxilasa y oxidorreductasa de aspergillus ochraceus AR031169A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US24430000P 2000-10-30 2000-10-30

Publications (1)

Publication Number Publication Date
AR031169A1 true AR031169A1 (es) 2003-09-10

Family

ID=22922180

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010105071A AR031169A1 (es) 2000-10-30 2001-10-30 11 alfa-hidroxilasa y oxidorreductasa de aspergillus ochraceus

Country Status (12)

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US (2) US7033807B2 (es)
EP (1) EP1352054A2 (es)
JP (1) JP2005512502A (es)
CN (1) CN1531590A (es)
AR (1) AR031169A1 (es)
AU (1) AU2002241768A1 (es)
BR (1) BR0115245A (es)
CA (1) CA2427615A1 (es)
IL (1) IL155529A0 (es)
MX (1) MX245674B (es)
PE (1) PE20020535A1 (es)
WO (1) WO2002046386A2 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006016702A1 (en) 2004-08-12 2006-02-16 Suntory Limited Method for polyunsaturated fatty acid production using novel cell preservation technique
JP5028275B2 (ja) * 2005-01-25 2012-09-19 バイエル・クロップサイエンス・アーゲー 亜リン酸、ホセチル−Ａｌ又は両者を同時に分析する方法
CN102827913B (zh) * 2012-08-31 2014-01-15 湖南成大生物科技有限公司 微生物混菌发酵制备11α-OH-ADD的方法
CN103627721B (zh) * 2013-10-22 2015-08-05 浙江工业大学 G6PDH基因在提高黑根霉对甾体C11α-羟基化能力中的应用及菌株
US10655133B2 (en) 2014-01-21 2020-05-19 Synplogen Co., Ltd. Method for preparing DNA unit composition, and method for creating concatenated DNA
CN104388515A (zh) * 2014-11-28 2015-03-04 江西赣亮医药原料有限公司 一种植物甾醇混菌发酵生产11ɑ-OH-ADD的方法
CN105039470B (zh) * 2015-08-03 2018-10-09 华南理工大学 一种干酪乳杆菌降解亚硝酸盐的电子供体蛋白及其制备方法和应用
ES2648614B1 (es) * 2016-05-30 2018-10-15 Consejo Superior De Investigaciones Científicas (Csic) Producción de esteroides 11-alfa hidroxilados mediante biotransformación con bacterias recombinantes
US20190264253A1 (en) * 2016-10-19 2019-08-29 Molecular Warehouse, Ltd. Ultrasensitive electrochemical biosensors
JP2019535689A (ja) * 2016-10-27 2019-12-12 バイエル アクチェンゲゼルシャフトＢａｙｅｒ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ４，５−縮環１，２，４−トリアゾロン
CN112424338A (zh) * 2018-07-17 2021-02-26 科纳根公司 γ-内酯的生物合成制备
CN112771652A (zh) * 2018-09-05 2021-05-07 安必昂公司 裸晶附接系统及用此系统进行集成精度验证和校准的方法
CN109182143B (zh) * 2018-09-28 2021-09-24 河南农业大学 一种烟曲霉lsd-1及其培养方法和应用
CN110499254B (zh) * 2019-07-22 2022-05-06 吉林大学 一种抗盐碱赭曲霉菌株w1及其菌剂和应用
CN110343622B (zh) * 2019-07-26 2021-01-01 兰州理工大学 产纤维素酶的甘草内生菌及其所产酶在提取甘草中甘草酸的应用
CN110713941A (zh) * 2019-10-22 2020-01-21 上海应用技术大学 高表达细胞色素p450单加氧酶赭曲霉菌株及其构建方法与应用
CN115335514A (zh) * 2019-10-25 2022-11-11 银杏生物制品公司 罗汉果甙的生物合成
CN113583984B (zh) * 2021-05-28 2023-05-23 湖北大学 一种细胞色素p450单加氧酶cyp109b2及其应用
CN114456952B (zh) * 2022-02-22 2023-04-14 昆明理工大学 一种金孢菌及其应用
CN115074293B (zh) * 2022-07-16 2024-05-28 山东金洋药业有限公司 一种甘油葡糖苷提纯工艺

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4419446A (en) * 1980-12-31 1983-12-06 The United States Of America As Represented By The Department Of Health And Human Services Recombinant DNA process utilizing a papilloma virus DNA as a vector
US4559332A (en) * 1983-04-13 1985-12-17 Ciba Geigy Corporation 20-Spiroxanes and analogues having an open ring E, processes for their manufacture, and pharmaceutical preparations thereof
US4588683A (en) * 1984-02-06 1986-05-13 Eastman Kodak Company Method of preparing 11β, 17α, 20, 21-tetrahydroxy steroids and corresponding 11β, 17α, 21-trihydroxy-20-oxo steroids
US4935233A (en) * 1985-12-02 1990-06-19 G. D. Searle And Company Covalently linked polypeptide cell modulators
US5869283A (en) * 1988-05-06 1999-02-09 Roussel Uclaf Expression cassette operable in a recombinant host
DE69113795T2 (de) * 1990-04-30 1996-03-28 Board of Regents, the University of Texas System, Austin, Tex. Steroid 5-alpha-reduktase.
US5384253A (en) * 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5348886A (en) * 1992-09-04 1994-09-20 Monsanto Company Method of producing recombinant eukaryotic viruses in bacteria
WO1997021830A1 (en) * 1995-12-12 1997-06-19 Akzo Nobel N.V. MICROBIAL 11α-HYDROXYLATION OF STEROIDS
ATE375992T1 (de) 1996-12-11 2007-11-15 Searle Llc Epoxidierungsverfahren
SE0302534L (sv) 2003-09-23 2005-03-08 Haldex Brake Prod Ab Beläggfjäder

Also Published As

Publication number Publication date
WO2002046386A2 (en) 2002-06-13
AU2002241768A1 (en) 2002-06-18
US20030148420A1 (en) 2003-08-07
IL155529A0 (en) 2003-11-23
MX245674B (es) 2007-05-07
US20050003473A1 (en) 2005-01-06
JP2005512502A (ja) 2005-05-12
US7033807B2 (en) 2006-04-25
CN1531590A (zh) 2004-09-22
WO2002046386A3 (en) 2003-08-07
CA2427615A1 (en) 2002-06-13
MXPA03003809A (es) 2003-07-28
US7238507B2 (en) 2007-07-03
BR0115245A (pt) 2005-05-10
EP1352054A2 (en) 2003-10-15
PE20020535A1 (es) 2002-06-05

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