CN112190291B - Quantitative sampling device for collecting intestinal microecological samples of piglets - Google Patents

Quantitative sampling device for collecting intestinal microecological samples of piglets Download PDF

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Publication number
CN112190291B
CN112190291B CN202011090014.9A CN202011090014A CN112190291B CN 112190291 B CN112190291 B CN 112190291B CN 202011090014 A CN202011090014 A CN 202011090014A CN 112190291 B CN112190291 B CN 112190291B
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sampling
collecting
sleeve
sealing
pipe
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CN112190291A (en
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郭晓红
高鹏飞
曹果清
李步高
杨阳
蔡春波
赵燕
路畅
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Shanxi Agricultural University
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Shanxi Agricultural University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/02—Instruments for taking cell samples or for biopsy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B2010/0061—Alimentary tract secretions, e.g. biliary, gastric, intestinal, pancreatic secretions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention provides a quantitative sampling device for collecting microecological samples of intestinal tracts of piglets, which comprises a sampling tube, an opening and closing assembly and a collecting assembly, wherein the head part of the sampling tube is provided with a sampling hole, the opening and closing assembly comprises a sealing head, a traction guide wire and an adjusting ring, the sealing head is arranged in the sampling tube, a sealing column and a sampling channel are arranged on the sealing head, the adjusting ring is clamped at the tail end of the sampling tube, one end of the traction guide wire is connected with the sealing head, the other end of the traction guide wire is connected with the adjusting ring, the collecting assembly comprises a piston and a collecting tube, the piston is arranged in the sampling tube, a first sleeve and a push plate are arranged on the piston, a second sleeve is arranged on the push plate, the second sleeve is arranged in the first sleeve, the collecting tube is arranged in the piston, a fan-shaped hole and a fan-shaped sealing plate are arranged on the fan-shaped sealing plate, the rotating rod is arranged in the second sleeve, a third sleeve is arranged at the tail end of the collecting tube, and the third sleeve is sleeved outside the first sleeve; the invention has reasonable structural design, high efficiency and convenience and is suitable for mass popularization.

Description

Quantitative sampling device for collecting intestinal microecological samples of piglets
Technical Field
The invention relates to the technical field of sample collection equipment, in particular to a quantitative sampling device for collecting intestinal microecological samples of piglets.
Background
In recent years, related researches show that intestinal flora is closely related to the growth and development of a host and physiological activities, and the intestinal flora participates in physiological functions including immunity, energy metabolism and the like. The animal intestinal tract is the largest immune organ in the body, and is also an independent organ which is obtained by the body in the acquired days and is different from the animal intestinal tract. The microorganisms planted in the intestinal tract of the host have no clear limit of interest and disadvantage in terms of the effect on the host, on one hand, the balance between the microorganisms in the intestinal tract of the host and the immune system is important, the microorganisms existing in the intestinal tract play a necessary role in perfecting the immune system of the intestinal tract, and the microorganisms are counterbalanced when harmful bacteria compete for the planting site, so that invasion of the harmful bacteria in the intestinal tract is reduced; on the other hand, microorganisms in the intestinal tract compete with the body in the distribution of nutrients, thereby consuming a portion of the body's nutrients.
The intestinal microecology of piglets mainly comprises 4 parts: (1) intestinal wall, including lumen of intestinal tract; (2) microorganisms in the intestinal tract; (3) intestinal secretions; (4) The food entering the intestinal tract affects the health of the body in combination with the above four parts, wherein the important function is microorganisms in the intestinal tract. The intestinal microecology has close relation with the growth and development of piglets, the intestinal tracts of the piglets provide a better growth environment for microbial colonization, and the interaction between the microorganisms in the intestinal tracts and the piglets can improve the digestion and absorption of nutrient substances, regulate the energy balance, regulate and control the immune response of the piglets, promote the piglets to form mature and stable microecology after weaning, and have a key effect on the healthy growth of the piglets.
The piglet intestinal microecological sample collection device in the prior art has the following defects in the using process: firstly, the quantitative sampling cannot be performed, and the sampling is performed only by the experience of researchers; secondly, the collected sample is easily influenced by external environment and is not representative; thirdly, the operation is inconvenient, and the intestinal tract of the piglet is extremely easy to be injured.
Disclosure of Invention
Aiming at the technical problems, the invention provides the quantitative sampling device for collecting the microecological samples of the intestinal tracts of the piglets, which is convenient to operate, safe and reliable.
The technical scheme of the invention is as follows: a quantitative sampling device for collecting intestinal microecological samples of piglets comprises a sampling tube, an opening and closing assembly and a collecting assembly; the head end of the sampling tube is of a hemispherical structure and is provided with a sampling hole; the opening and closing assembly comprises a sealing head, a traction guide wire and an adjusting ring, wherein the sealing head is movably sleeved in the sampling tube, one side, close to the head end of the sampling tube, of the sealing head is provided with a sealing column and a sampling channel, the sampling channel is positioned at the center of the sealing head, the sealing columns are uniformly arranged at the outer side of the sampling channel, the sealing columns are corresponding to the sampling holes in number and are consistent, the sampling holes can be penetrated, the adjusting ring is movably clamped at the tail end of the sampling tube, one end of the traction guide wire is fixedly connected with the sealing head, and the other end of the traction guide wire penetrates through the inner wall of the sampling tube and is fixedly connected with the adjusting ring; the collecting assembly comprises a piston and a collecting pipe, the piston is movably sleeved inside the sampling pipe, a first sleeve is arranged on one side, close to the tail end of the sampling pipe, of the piston, the first sleeve penetrates through the adjusting ring, a push plate is clamped on one side, close to the head end of the sampling pipe, of the piston, a second sleeve is connected to the push plate, the second sleeve penetrates through the rear end of the piston and sleeved inside the first sleeve, the collecting pipe is movably sleeved inside the piston, a fan-shaped hole is formed in one side, close to the head end of the sampling pipe, of the collecting pipe, a fan-shaped sealing plate is rotatably clamped inside the collecting pipe, a rotating rod is connected to the fan-shaped sealing plate, the rotating rod is movably sleeved inside the second sleeve, 5-8 guide rods are uniformly arranged on one side, close to the tail end of the sampling pipe, of the collecting pipe penetrates through the piston and then is connected with the bottom plate, the bottom plate penetrates through the first sleeve, a third sleeve is arranged on the bottom plate, the third sleeve is sleeved outside the first sleeve, and scales are arranged on the third sleeve, and the lengths of the first sleeve, the second sleeve and the rotating rod are sequentially increased.
Further, still include locating component, locating component includes locating plate and regulating ball, and regulating ball movable sleeve is established at the sampling pipe tail end, is provided with the arc draw-in groove on the locating plate, is provided with arc draw-in groove and regulating ball activity joint on the locating plate, is provided with elastic bandage on the locating plate, fixes sampling pipe and porkling through the bandage, utilizes the rotation of regulating ball and arc draw-in groove to be connected simultaneously to adjust the angle of sampling pipe, makes the sampling pipe get into in the porkling smoothly.
Further, be provided with the sliding tray on the sampling pipe head end inside wall, the sealed head outside is provided with the fixture block, and sealed head passes through fixture block and sliding tray activity joint, pulls the one end and the fixture block fixed connection of seal wire, through establishing sliding tray and fixture block for sealed head can not take place to rotate at the back-and-forth movement in-process, is favorable to improving the location of seal post and sampling hole more accurate.
Further, a reset spring is sleeved on the traction guide wire inside the sliding groove, one end of the reset spring is fixedly connected with the traction guide wire, the other end of the reset spring is abutted to one side, close to the tail end of the sampling tube, of the sliding groove, through the reset spring, the sealing column is beneficial to blocking and sealing the sampling hole after the use is finished, pollution caused by environmental pollution to intestinal microecological indexes of piglets is avoided, and accuracy of intestinal microecological research results is improved.
Further, the head end of the sampling tube is connected with the sampling tube through threads, so that the head end of the sampling tube is convenient to detach, and the sample in the collecting tube is convenient to transfer.
Further, threaded connection between adjusting ring and the sampling pipe, the inside rotation joint of adjusting ring has the swivel becket, and one side that the swivel becket is close to the sampling pipe is provided with the inserted bar, and the inserted bar activity is pegged graft on the sampling pipe lateral wall, pulls seal wire and inserted bar fixed connection for the adjusting ring rotates the in-process, and swivel becket horizontal pulling pulls the seal wire, avoids pulling the seal wire pulling in-process to tie knots and twines and influence the sealed effect of sealing head.
Further, the collecting pipe is made of a light-proof material, and the collecting pipe is in sealing connection with the piston and the fan-shaped sealing plate; the design can effectively avoid the influence of outside air on fungus in the piglet intestinal microecological sample.
The working principle of the invention is as follows: when in use, the head end of the sampling tube is placed into the anus of the piglet until the rectum of the piglet is positioned, and then the sampling tube is fixed with the piglet through an elastic binding band; rotating the adjusting ring to enable the rotating ring to be far away from the tail end of the sampling tube under the pushing action of the adjusting ring, and further enabling the traction guide wire to pull the sealing head to move towards the tail end of the sampling tube, and enabling the sealing column on the sealing head to be separated from the sampling hole; pulling the first sleeve to the tail end of the sampling tube to enable the piston and the collecting tube to move towards the tail end of the sampling tube, enabling the micro-ecological sample in the intestinal tract of the piglet to sequentially enter the sampling tube through the sampling hole and the sampling channel under the action of negative pressure, then reversely adjusting the ring, and enabling the sealing head to reseal the sampling hole by utilizing the reset spring; pushing the third sleeve to enable the collecting pipe to extend out of the piston, collecting samples in the sampling pipe, and quantifying the collected samples by utilizing scale marks on the third sleeve; after collection is completed, the rotating rod is rotated, so that the fan-shaped hole is sealed by the fan-shaped sealing plate, and the sample is in a sealed state; the device is separated from the piglet, the head end of the sampling tube is removed, the rotating rod is rotated again, the fan-shaped sealing plate is opened, the second sleeve is pushed, and the sample in the collecting tube is transferred by the second sleeve, so that the next monitoring study can be carried out.
Compared with the prior art, the invention has the beneficial effects that: the invention has reasonable structural design, is convenient for fixing the sampling tube by arranging the positioning component, and is convenient for adjusting the angle of the sampling tube by rotating and combining the adjusting ball and the house type clamping groove, so that the sampling tube can smoothly enter the intestinal canal of the piglet without damaging the piglet; in the process of collecting piglet intestinal microecology by using the sampling tube, the collected sample is always isolated from the external environment, so that the death caused by the pollution of anaerobic bacteria in the sample by the external environment is avoided, the accuracy and the reliability of sample detection are improved, and theoretical basis is provided for sow delivery protection and piglet early care and research and development of microbial preparations for improving piglet intestinal health.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic structural view of the seal head of the present invention;
FIG. 3 is an enlarged schematic view of the present invention at A in FIG. 1;
FIG. 4 is an enlarged schematic view of the present invention at B in FIG. 1;
FIG. 5 is a schematic illustration of the connection of a collection tube to a plunger of the present invention;
FIG. 6 is a schematic illustration of the connection of a fan-shaped seal plate of the present invention to a collection tube;
FIG. 7 is an enlarged schematic view of the present invention at C in FIG. 1;
the device comprises a 1-sampling tube, a 10-sampling hole, a 11-sliding groove, a 2-opening and closing component, a 20-sealing head, a 200-sealing column, a 201-sampling channel, a 202-clamping block, a 21-traction guide wire, a 210-reset spring, a 22-adjusting ring, a 220-rotating ring, a 221-inserting rod, a 3-collecting component, a 30-piston, a 300-first sleeve, a 301-pushing plate, a 302-second sleeve, a 31-collecting tube, a 310-sector hole, a 311-sector sealing plate, a 312-rotating rod, a 313-guiding rod, a 314-bottom plate, a 315-third sleeve, a 4-positioning component, a 40-positioning plate, a 400-arc clamping groove, a 401-elastic binding belt and a 41-adjusting ball.
Detailed Description
Examples: the quantitative sampling device for collecting the microecological samples of the intestinal tracts of the piglets as shown in figures 1 and 3 comprises a sampling tube 1, an opening and closing assembly 2, a collecting assembly 3 and a positioning assembly 4; the head end of the sampling tube 1 is of a hemispherical structure and is provided with a sampling hole 10, and the head end of the sampling tube 1 is connected with the sampling tube 1 through threads, so that the head end of the sampling tube 1 is convenient to detach, and samples in the collecting tube 31 are convenient to transfer; a sliding groove 11 is arranged on the inner side wall of the head end of the sampling tube 1;
as shown in fig. 1, 2, 3 and 4, the opening and closing assembly 2 comprises a sealing head 20, a traction guide wire 21 and an adjusting ring 22, wherein the sealing head 20 is movably sleeved inside the sampling tube 1, one side, close to the head end of the sampling tube 1, of the sealing head 20 is provided with a sealing column 200 and a sampling channel 201, the sampling channel 201 is positioned at the center of the sealing head 20, the sealing columns 200 are uniformly arranged outside the sampling channel 201, the number of the sealing columns 200 is correspondingly consistent with that of sampling holes 10, the sealing columns can penetrate through the sampling holes 10, the adjusting ring 22 is movably clamped at the tail end of the sampling tube 1, one end of the traction guide wire 21 is fixedly connected with the sealing head 20, and the other end of the traction guide wire penetrates through the inner wall of the sampling tube 1 and is fixedly connected with the adjusting ring 22; the clamping block 202 is arranged on the outer side of the sealing head 20, the sealing head 20 is movably clamped with the sliding groove 11 through the clamping block 202, one end of the traction guide wire 21 is fixedly connected with the clamping block 202, and the sliding groove 11 and the clamping block 202 are arranged, so that the sealing head 20 cannot rotate in the forward and backward movement process, and the sealing post 200 and the sampling hole 10 are positioned more accurately; a reset spring 210 is sleeved on the traction guide wire 21 positioned in the sliding groove 11, one end of the reset spring 210 is fixedly connected with the traction guide wire 21, the other end of the reset spring 210 is abutted against one side, close to the tail end of the sampling tube 1, of the sliding groove 11, and after the use is finished, the sealing column 200 is beneficial to blocking and sealing the sampling hole 10, so that pollution caused by environmental pollution to intestinal microecological indexes of piglets is avoided, and the accuracy of intestinal microecological research results is improved; the adjusting ring 22 is in threaded connection with the sampling tube 1, the rotating ring 220 is clamped in the adjusting ring 22 in a rotating way, the inserting rod 221 is arranged on one side, close to the sampling tube 1, of the rotating ring 220, the inserting rod 221 is movably inserted into the side wall of the sampling tube 1, the traction guide wire 21 is fixedly connected with the inserting rod 221, and the inserting rod 221 is arranged, so that the rotating ring 220 horizontally pulls the traction guide wire 21 in the rotating process of the adjusting ring 22, and the sealing effect of the sealing head 20 is prevented from being influenced by knotting and winding in the pulling process of the traction guide wire 21;
as shown in fig. 1, 3, 5, 6 and 7, the collecting assembly 3 comprises a piston 30 and a collecting pipe 31, the piston 30 is movably sleeved in the sampling pipe 1, a first sleeve 300 is arranged on one side, close to the tail end of the sampling pipe 1, of the piston 30, the first sleeve 300 penetrates through the adjusting ring 22, a push plate 301 is clamped on one side, close to the head end of the sampling pipe 1, of the piston 30, a second sleeve 302 is connected to the push plate 301, the second sleeve 302 penetrates through the piston 30 and is sleeved in the first sleeve 300, the collecting pipe 31 is movably sleeved in the piston 30, a fan-shaped hole 310 is arranged on one side, close to the head end of the sampling pipe 1, of the collecting pipe 31, a fan-shaped sealing plate 311 is rotatably clamped in the collecting pipe 31, a rotating rod 312 is connected to the fan-shaped sealing plate 311, the rotating rod 312 is movably sleeved in the second sleeve 302, 6 guide rods 313,6 are uniformly arranged on one side, close to the tail end of the collecting pipe 31, of the sampling pipe 1, the first sleeve 313 penetrates through the piston 30 and then is connected through a bottom plate 314, the bottom plate 314 penetrates through the first sleeve 300, a third sleeve 315 is arranged on the bottom plate 314, the third sleeve 315 is sleeved outside the first sleeve 300, a scale mark sleeve 315 is arranged on the third sleeve 315, and the first sleeve 315, the length of the first sleeve 300 and the second sleeve 315 is sequentially increased; the collecting pipe 31 is made of a light-proof material, and the collecting pipe 31 is in sealing connection with the piston 30 and the fan-shaped sealing plate 311; the design can effectively prevent fungus in the intestinal microecological sample of the piglet from being influenced by outside air;
as shown in fig. 1, the positioning assembly 4 comprises a positioning plate 40 and an adjusting ball 41, the adjusting ball 41 is movably sleeved at the tail end of the sampling tube 1, an arc clamping groove 400 is formed in the positioning plate 40, the arc clamping groove 400 is movably clamped with the adjusting ball 41 and is arranged on the positioning plate 40, an elastic binding band 401 is arranged on the positioning plate 40, the sampling tube 1 and a piglet are fixed through the binding band 4, and meanwhile, the angle of the sampling tube 1 is adjusted by rotating and connecting the adjusting ball 41 with the arc clamping groove 400, so that the sampling tube 1 smoothly enters the piglet.
When in use, the head end of the sampling tube 1 is placed into the anus of a piglet until the rectum of the piglet is positioned, and then the sampling tube 1 is fixed with the piglet through an elastic binding belt 401; rotating the adjusting ring 22 to enable the rotating ring 220 to be far away from the tail end of the sampling tube 1 under the pushing action of the adjusting ring 22, and further enabling the traction guide wire 21 to pull the sealing head 20 to move towards the tail end of the sampling tube 1, and enabling the sealing column 200 on the sealing head 20 to be separated from the sampling hole 10; pulling the first sleeve 300 towards the tail end of the sampling tube 1 to enable the piston 30 and the collecting tube 31 to move towards the tail end of the sampling tube 1, at the moment, under the action of negative pressure, the micro-ecological sample of the piglet intestinal canal sequentially passes through the sampling hole 10 and the sampling channel 201 to enter the sampling tube 1, and then reversely adjusting the ring 22, and enabling the sealing head 20 to reseal the sampling hole 10 by utilizing the reset spring 210; pushing the third sleeve 315 to enable the collecting pipe 31 to extend out of the piston 30, collecting samples in the sampling pipe 1, and quantifying the collected samples by utilizing scale marks on the third sleeve 315; after collection is completed, the rotating rod 312 is rotated, so that the fan-shaped sealing plate 311 seals the fan-shaped hole 310, and the sample is in a sealed state; the device is separated from the piglet, the head end of the sampling tube 1 is removed, the rotating rod 312 is rotated again, the fan-shaped sealing plate 311 is opened, the second sleeve 302 is pushed, and the second sleeve 302 is utilized to transfer the sample in the collecting tube 31, so that the next monitoring study can be carried out.

Claims (7)

1. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of the piglets is characterized by comprising a sampling tube (1), an opening and closing assembly (2) and a collecting assembly (3); the head end of the sampling tube (1) is of a hemispherical structure and is provided with a sampling hole (10); the opening and closing assembly (2) comprises a sealing head (20), traction guide wires (21) and an adjusting ring (22), wherein the sealing head (20) is movably sleeved inside the sampling tube (1), one side, close to the head end of the sampling tube (1), of the sealing head (20) is provided with a sealing column (200) and a sampling channel (201), the sampling channel (201) is located at the central position of the sealing head (20), the sealing columns (200) are uniformly arranged outside the sampling channel (201), the number of the sealing columns (200) is correspondingly consistent with that of the sampling holes (10), the sealing columns can penetrate through the sampling holes (10), the adjusting ring (22) is movably clamped at the tail end of the sampling tube (1), one end of the traction guide wires (21) is fixedly connected with the sealing head (20), and the other end of the traction guide wires (21) penetrates through the inner wall of the sampling tube (1) and then is fixedly connected with the adjusting ring (22). The collecting assembly (3) comprises a piston (30) and a collecting pipe (31), the piston (30) is movably sleeved inside the sampling pipe (1), one side, close to the tail end of the sampling pipe (1), of the piston (30) is provided with a first sleeve (300), the first sleeve (300) penetrates through an adjusting ring (22), one side, close to the head end of the sampling pipe (1), of the piston (30) is clamped with a push plate (301), the push plate (301) is connected with a second sleeve (302), the second sleeve (302) penetrates through the piston (30) and is sleeved inside the first sleeve (300), the collecting pipe (31) is movably sleeved inside the piston (30), one side, close to the head end of the sampling pipe (1), of the collecting pipe (31) is provided with a sector hole (310), the inner rotating clamp of the collecting pipe (31) is provided with a sector sealing plate (311), the sector sealing plate (311) is connected with a rotating rod (312), one side, close to the tail end of the sampling pipe (1), of the collecting pipe (31) is uniformly provided with 5-8 guide rods (313-8) which penetrate through the first sleeve (313) and the bottom plate (313), be provided with third sleeve pipe (315) on bottom plate (314), third sleeve pipe (315) cover is established in first sleeve pipe (300) outside, is provided with scale mark on third sleeve pipe (315), and the length of third sleeve pipe (315), first sleeve pipe (300), second sleeve pipe (302) and dwang (312) increases in proper order.
2. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of pigs according to claim 1, further comprising a positioning assembly (4), wherein the positioning assembly (4) comprises a positioning plate (40) and an adjusting ball (41), the adjusting ball (41) is movably sleeved at the tail end of the sampling tube (1), an arc-shaped clamping groove (400) is formed in the positioning plate (40), the arc-shaped clamping groove (400) is movably clamped with the adjusting ball (41) on the positioning plate (40), and an elastic binding belt (401) is arranged on the positioning plate (40).
3. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of pigs according to claim 1, wherein a sliding groove (11) is formed in the inner side wall of the head end of the sampling tube (1), a clamping block (202) is arranged on the outer side of the sealing head (20), the sealing head (20) is movably clamped with the sliding groove (11) through the clamping block (202), and one end of the traction guide wire (21) is fixedly connected with the clamping block (202).
4. A quantitative sampling device for collecting microecological samples of the intestinal tracts of pigs according to claim 3, characterized in that a return spring (210) is sleeved on a traction guide wire (21) positioned in the sliding groove (11), one end of the return spring (210) is fixedly connected with the traction guide wire (21), and the other end of the return spring is abutted against one side, close to the tail end of the sampling tube (1), of the sliding groove (11).
5. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of pigs according to claim 1, wherein the head end of the sampling tube (1) is connected with the sampling tube (1) through threads.
6. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of the piglets according to claim 1, wherein the collecting pipe (31) is made of a light-proof material, and the collecting pipe (31) is in sealing connection with the piston (30) and the fan-shaped sealing plate (311).
7. The quantitative sampling device for collecting the microecological samples of the intestinal tracts of pigs according to claim 1, wherein the sampling tube (1) is in threaded connection with the head end.
CN202011090014.9A 2020-10-13 2020-10-13 Quantitative sampling device for collecting intestinal microecological samples of piglets Active CN112190291B (en)

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CN113440181B (en) * 2021-07-06 2022-07-12 中南大学湘雅医院 Biological tissue biopsy sampling device based on endoscopic system

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