CN110448336A - A kind of device and application method of autonomous acquisition intestinal microecology sample - Google Patents
A kind of device and application method of autonomous acquisition intestinal microecology sample Download PDFInfo
- Publication number
- CN110448336A CN110448336A CN201910772569.2A CN201910772569A CN110448336A CN 110448336 A CN110448336 A CN 110448336A CN 201910772569 A CN201910772569 A CN 201910772569A CN 110448336 A CN110448336 A CN 110448336A
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- main cartridge
- sample
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- internal box
- glycerol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0038—Devices for taking faeces samples; Faecal examination devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01G—WEIGHING
- G01G17/00—Apparatus for or methods of weighing material of special form or property
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01G—WEIGHING
- G01G17/00—Apparatus for or methods of weighing material of special form or property
- G01G17/04—Apparatus for or methods of weighing material of special form or property for weighing fluids, e.g. gases, pastes
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- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses the device and application method of a kind of autonomous acquisition intestinal microecology sample, device includes outer box, main cartridge and internal box, outer box bottom is connect with toilet seat, main cartridge can opposite outer box body slide up and down, the bottom of internal box is fixedly connected with outer box bottom surface, top is protruded into inside it behind the bottom surface of main cartridge, sealing cover is connected at the top of main cartridge, sealing film is equipped with above main cartridge, a cotton swab is connected by sliding bar at the top of main cartridge, the gravity sensor and alarm of electrical connection are equipped in the sample continuing surface bottom of internal tray interior, the glycerol gatherer for being equipped on main cartridge outer wall and being connected to inside internal box.The structure of the device is simple, the time of contact of strong operability, the intestinal microecology sample and outside air that are obtained using the device is few, and needs not move through the transition of the external tools such as bedpan, reduce a possibility that sample is contaminated as far as possible, guarantees that sample Tiny ecosystem structure is undistorted.
Description
Technical field
The present invention relates to a kind of sample collecting apparatus, and in particular to a kind of autonomous device of acquisition intestinal microecology sample and
Application method.
Background technique
Human body microecosystem includes four oral cavity, skin, uropoiesis, gastrointestinal tract microecosystems.With intestinal microecology system
System is main, the most complicated.The most significant feature of intestinal flora first is that its stability, it resists enteropathogenic to the mankind
Microbial infectious diseases is extremely important.Maintaining its stability is the emphasis of clinical treatment.
Intestinal microecology can be used as the Xiang Zhibiao for judging human health status, be put into the inspection of intestinal microecology
In the physical examination project of corresponding crowd.The people that need to carry out intestinal microecology detection is firstly the need of acquisition excrement, conventional acquisition mode
It is to dip a small amount of excrement after excrement is drained into bedpan with the small spoon in excrement collector and place inspection in the vial.
The intestinal microecology detection mainly composition ratio of detection enteric microorganism such as bacterium, however utilize above-mentioned sampling side
Excrement can expose for a long time in air during formula is sampled, and anaerobic bacteria is dead at this time, and aerobic bacteria then can be after
Continuous breeding, causes testing result to be distorted.In addition, excrement is discharged into bedpan by patient, the microorganism in bedpan equally can be to excrement
Sample pollutes, this also will affect testing result.
Therefore, in order to collect the sample of authentic representative intestinal microecology, a kind of sample and empty of capable of reducing to the greatest extent need to be developed
Gas and other articles contact possible acquisition instrument.
Summary of the invention
To solve the above-mentioned problems, the present invention provides device and the user of the autonomous acquisition intestinal microecology sample of one kind
Method, structure is simple, easy to operate, can be with precise acquisition enteric microorganism sample.
To realize above-mentioned technical purpose and the technique effect, the present invention is achieved by the following technical solutions: one
The device of the autonomous acquisition intestinal microecology sample of kind, including the outer box, main cartridge and inner cartridge set gradually from outside to inside
Body, outer box bottom are connect with toilet seat screw top, main cartridge can opposite outer box body slide up and down, the bottom of internal box
Portion is fixedly connected with the inner bottom surface of outer box, top extendes back across the bottom surface of main cartridge and becomes owner of tray interior, in main cartridge
Screw top connects sealing cover, and the position above main cartridge and lower than box body top surface is equipped with sealing film, at the top of main cartridge
A cotton swab is connected by sliding bar, the side wall that the outboard end of sliding bar passes through main cartridge stretches out outside main cartridge, main cartridge, inside
Box body and cotton swab coaxial arrangement are equipped with gravity sensor and the alarm of electrical connection in the sample continuing surface bottom of internal tray interior
Device is equipped with and the glycerol gatherer that is connected to inside internal box on main cartridge outer wall.
Further, several embedded telescopic mast receiving holes are equipped in main cartridge bottom symmetrical, on telescopic mast receiving hole top
It is equipped with telescopic mast between face and outer box bottom surface, spring, the top and telescopic mast receiving hole top surface of spring are coated on telescopic mast
It is fixedly connected, bottom end is fixedly connected with outer box bottom surface.
Further, the glycerol gatherer includes protective cover, sealing cover two, glycerol tube, imports hard tube and connect soft
Pipe, sealing cover two are connected to the outer openings end of protective cover, and glycerol tube is located in protective cover, and glycerol tube is provided with glycerol and glycerol
Pipe is squeezable cystic structures, imports hard tube one end and the outlet end connection of glycerol tube, the other end protrude into inside main cartridge, In
Import in hard tube and be equipped with diaphragm close to the side of glycerol tube, connect one end of hose with import hard tube connect, the other end protrude into it is interior
In portion's box body.
Further, it is equipped with limit ring wall in the interior sidewall surface of internal box, limits the bottom opening of ring wall, connects soft
It is protruded into internal box behind the space that pipe is enclosed by limit ring wall and internal box wall surface.
Further, it is equipped with limited block in the outboard end of sliding bar, locating piece, limit is equipped on the lateral surface of main cartridge
Block and locating piece leading flank against when cotton swab it is in a vertical state.
Further, it is equipped in the junction and importing hard tube of internal box and main cartridge and the junction of main cartridge
Sealing ring.
Further, the top ring week of the main cartridge and internal box is equipped with abrasionproof bound edge.
Further, it is equal in main cartridge bottom surface to outer box at the top of the internal box to the distance at the top of main cartridge
The distance of portion bottom surface.
A kind of application method of the device of autonomous acquisition intestinal microecology sample:
1) outer box bottom is threadedly coupled with toilet seat, sealing cover is opened, keep cotton swab in a vertical state；
2) rotation sliding bar makes cotton swab lodge and pulls out sliding bar after cotton swab is directed at anus by sampling person；
3) sampling person's buttocks pushes, main cartridge opposite outer box body slide downward, at this point, internal box slides up with respect to main cartridge
Dynamic, internal box top surface is broken through sealing film and is flushed to its top surface with main cartridge top surface；
4) normal defecation, excrement are fallen on the sample continuing surface of internal tray interior, reach inspection in the weight of the excrement fallen
Survey is taken, and gravity sensor touches alarm equipment alarm；
5) then, sampling person opens sealing cover two, squeezes glycerol tube, so that the glycerol in glycerol tube is broken through diaphragm, pass sequentially through and lead
Enter in hard tube and inflow internal box after connection hose and covers fecal sample；
6) it finally, the buttocks of sampling person leaves sample collecting device, can move backward to continue defecation on toilet seat, in defecation
During sliding bar can be resetted to cover sealing cover, outer box removed to complete from toilet seat entirely take
Sample process.
The beneficial effects of the present invention are:
1, the structure of the intestinal microecology sample sampler is simple, and preparation cost is low, and easy to use, strong operability；
2, using the device obtain intestinal microecology sample and outside air time of contact it is few, reduce as far as possible sample by
To a possibility that pollution, the influence of Tiny ecosystem testing result will be preferably minimized；Guarantee that the Tiny ecosystem structure of excrement will not be because taking
Just process and detail are distorted；
3, excrement is expelled directly out in sampler, needs not move through the transition of the external tools such as bedpan, is further reduced sample quilt
The possibility of pollution.
Detailed description of the invention
Fig. 1 is under the device and toilet seat connection status of a kind of autonomous acquisition intestinal microecology sample disclosed by the invention
Structural schematic diagram；
Fig. 2 is a kind of structural schematic diagram of the device of autonomous acquisition intestinal microecology sample disclosed by the invention
Fig. 3 is the enlarged drawing of part A in Fig. 2.
Wherein, 1- outer box, 2- main cartridge, 3- internal box, 4- toilet seat, 5- sealing cover, 6- sealing film, 7- are sliding
Lever, 8- cotton swab, 9- gravity sensor, 10- alarm, 11- glycerol gatherer, 12- telescopic mast receiving hole, 13- telescopic mast,
14- spring；
111- protective cover, 112- sealing cover two, 113- glycerol tube, 114- importing hard tube, 115- connection hose, 116- diaphragm,
117- limits ring wall, 118- limited block, 119- locating piece.
Specific embodiment
The preferred embodiments of the present invention will be described in detail by 1-3 with reference to the accompanying drawing, so that advantages of the present invention and spy
Sign can be easier to be readily appreciated by one skilled in the art, so as to make a clearer definition of the protection scope of the present invention.
In order to collect can with the sample of accurate representation intestinal microecology situation, to avoid sample in collection process with
Outside air contact for a long time causes sample distortion and the pollution by the outer transitions utensil such as bedpan, and the present invention discloses one kind certainly
It is main acquisition intestinal microecology sample device, the device need and dedicated toilet seat be used cooperatively, the main structure of toilet seat with
Common toilet seat is identical, only an embedded threaded hole need to be arranged in the front side end of toilet seat upper surface and adopt for connecting sample
Acquisition means.
The device of the autonomous acquisition intestinal microecology sample did sterilization treatment before being not used, specific structure include from
Outer box 1, main cartridge 2 and the internal box 3 set gradually outside in, 1 bottom of outer box is equipped with connecting pin, in connecting pin
Surface is equipped with external screw thread, is equipped with the interior spiral shell being adapted to external screw thread on connecting pin in the embedded connecting hole on toilet seat upper surface
Line, the outer box 1 are connect with 4 screw top of toilet seat, main cartridge 2 can opposite outer box body 1 slide up and down, internal box
3 bottom is fixedly connected with the inner bottom surface of outer box 1, top extendes back inside main cartridge 2 across the bottom surface of main cartridge 2,
It is equipped with external screw thread on 2 top peripheral side wall of main cartridge and is used for seal with screwed joint lid 5, above main cartridge 2 and is lower than box body
The position of top surface is equipped with sealing film 6, is located above sealing film 6 at the top of main cartridge 2 and passes through horizontally disposed cylindrical cunning
Lever 7 connects a cotton swab 8, and the side wall that the outboard end of sliding bar 7 passes through main cartridge 2 stretches out outside main cartridge 2, in internal box 3
Internal sample continuing surface bottom is equipped with the gravity sensor 9 and alarm 10 of electrical connection, be equipped on 2 outer wall of main cartridge with it is interior
The glycerol gatherer 11 being connected to inside portion's box body 3.
Excrement can accurately fall into inner cartridge when main cartridge 2, internal box 3 and the coaxial arrangement of cotton swab 8 are to guarantee sample collection
In body 3.
2 bottom symmetrical of main cartridge be equipped with several embedded telescopic mast receiving holes 12,12 top surface of telescopic mast receiving hole with
It is equipped with telescopic mast 13 between 1 bottom surface of outer box, spring 14, the top of spring 14 and telescopic mast receiving hole are coated on telescopic mast 13
12 top surfaces are fixedly connected, bottom end is fixedly connected with 1 bottom surface of outer box.
The glycerol gatherer 11 includes protective cover 111, sealing cover 2 112, glycerol tube 113, imports hard tube 114 and connect
Hose 115 is connect, sealing cover 2 112 is connected to the outer openings end of protective cover 111, and glycerol tube 113 is located in protective cover 111, sweet
Oil pipe 113 is provided with glycerol and glycerol tube 113 is squeezable cystic structures, and importing 114 one end of hard tube goes out with glycerol tube 113
The connection of mouth end, the other end protrude into inside main cartridge 2, are equipped with diaphragm 116 close to the side of glycerol tube 113 importing in hard tube 114,
Hard tube 114 is connect, the other end protrudes into internal box 3 with importing for one end of connection hose 115.
It is equipped with limit ring wall 117 in the interior sidewall surface of internal box 3, limits the bottom opening of ring wall 117, connects hose
It is protruded into internal box 3 behind 115 spaces being enclosed by limit ring wall 117 and 3 wall surface of internal box, which can guarantee
Connection hose 115 in internally positioned box body 3 welt always, will not influence excrement and is discharged into.
It is equipped with limited block 118 in the outboard end of sliding bar 7, locating piece 119, limit are equipped on the lateral surface of main cartridge 2
Block 118 and 119 leading flank of locating piece against when cotton swab 8 it is in a vertical state.
It is equipped in the junction of internal box 3 with the junction of main cartridge 2 and importing hard tube 114 and main cartridge 2 close
Seal reduces the chance that sample is contacted with outside air as far as possible, prevents detection from tying to improve the air-tightness inside internal box 3
Fruit distortion.
The top ring week of the main cartridge 2 and internal box 3 is equipped with abrasionproof bound edge specifically can be in main cartridge 2 and inside
The flexible materials such as the one circle non-woven fabrics of top open part cladding of box body 3, the design is primarily to prevent main cartridge 2 and inner cartridge
The top of body 3 and sampling person's buttocks skin scratch sampling person when contacting.
Distance at the top of the internal box 3 to 2 top of main cartridge is equal to 2 bottom surface of main cartridge to 1 inner bottom of outer box
The distance in face.
The specifically used method of the device are as follows:
1) connecting pin of 1 bottom of outer box is connect with 4 screw top of toilet seat, sealing cover 5 is opened, be in cotton swab 8
Vertical state；
2) rotation sliding bar 7 after the alignment anus of cotton swab 8 is made the lodging of cotton swab 8 by sampling person and pulling out sliding bar 7 keeps cotton swab inclined
Axis position from internal box 3 falls into avoid excrement is influenced；
3) sampling person pushes buttocks, 1 slide downward of opposite outer box body under the action of human bady gravitational of main cartridge 2, at this point, internal
Box body 3 with respect to 2 upward sliding of main cartridge, break through sealing film 6 and flush to the top surface of main cartridge 2 by 3 top surface of internal box；
4) normal defecation, excrement are fallen on the sample continuing surface inside internal box 3, reach inspection in the weight of the excrement fallen
Survey is taken, and gravity sensor 9 triggers alarm 10 and alarms；
5) then, sampling person opens sealing cover 2 112, squeezes glycerol tube 113, the glycerol in glycerol tube 113 is made to break through diaphragm
116, glycerol, which passes sequentially through to import in hard tube 114 and inflow internal box 3 after connection hose 115, covers fecal sample, prevents
Bacterium in excrement influences the testing result of Tiny ecosystem because being exposed to air；
6) it finally, the buttocks of sampling person leaves sample collecting device, can move backward to continue defecation on toilet seat, in defecation
During can by sliding bar 7 reset to cover sealing cover 5, outer box 1 is removed from toilet seat 4 complete it is whole
A sampling process.
The acquisition of intestinal microecology sample is carried out using above-mentioned apparatus and method, maximizing prevents sample to be distorted, and guarantees
The accuracy of testing result, and overall structure is simple, easy to operate, sampling person can independent completion sampling process.
The above description is only an embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills
Art field, is included within the scope of the present invention.
Claims (9)
1. a kind of device of autonomous acquisition intestinal microecology sample, which is characterized in that including the outside set gradually from outside to inside
Box body (1), main cartridge (2) and internal box (3), outer box (1) bottom are connect with toilet seat (4) screw top, main cartridge
(2) can opposite outer box body (1) slide up and down, the inner bottom surface of the bottom of internal box (3) and outer box (1) is fixed to be connected
It connects, top is extended back across the bottom surface of main cartridge (2) into main cartridge (2) inside；Sealing cover is connected in main cartridge (2) screw top
(5), the position above main cartridge (2) and lower than box body top surface is equipped with sealing film (6), passes through water at the top of main cartridge (2)
Flat sliding bar (7) connects a cotton swab (8), and the side wall that the outboard end of sliding bar (7) passes through main cartridge (2) stretches out main cartridge
(2) outside, main cartridge (2), internal box (3) and cotton swab (8) coaxial arrangement, at the internal sample continuing surface bottom of internal box (3)
Portion is equipped with the gravity sensor (9) and alarm (10) of electrical connection, is equipped on main cartridge (2) outer wall interior with internal box (3)
The glycerol gatherer (11) of portion's connection.
2. a kind of device of autonomous acquisition intestinal microecology sample as described in claim 1, which is characterized in that in main cartridge
(2) bottom symmetrical is equipped with several embedded telescopic mast receiving holes (12), in telescopic mast receiving hole (12) top surface and outer box (1)
Telescopic mast (13) are equipped between bottom surface, coat spring (14) on telescopic mast (13), the top of spring (14) and telescopic mast receiving hole
(12) top surface is fixedly connected, bottom end is fixedly connected with outer box (1) bottom surface.
3. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 2, which is characterized in that the glycerol is led
Enter device (11) to include protective cover (111), sealing cover two (112), glycerol tube (113), import hard tube (114) and connection hose
(115), sealing cover two (112) is connected to the outer openings end of protective cover (111), and glycerol tube (113) is located at protective cover (111)
Interior, it is squeezable cystic structures that glycerol tube (113), which is provided with glycerol and glycerol tube (113), import hard tube (114) one end with it is sweet
Main cartridge (2) inside is protruded into the outlet end connection of oil pipe (113), the other end, is being imported in hard tube (114) close to glycerol tube
(113) side is equipped with diaphragm (116), connect the one end of hose (115) with import hard tube (114) connect, the other end protrude into it is interior
In portion's box body (3).
4. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 3, which is characterized in that in internal box
(3) interior sidewall surface is equipped with limit ring wall (117), limits the bottom opening of ring wall (117), and connection hose (115) passes through limit
It is protruded into behind the space that position ring wall (117) and internal box (3) wall surface are enclosed in internal box (3).
5. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 4, which is characterized in that in sliding bar
(7) outboard end be equipped with limited block (118), on the lateral surface of main cartridge (2) be equipped with locating piece (119), limited block (118) with
Locating piece (119) leading flank against when cotton swab (8) it is in a vertical state.
6. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 5, which is characterized in that in internal box
(3) junction with the junction of main cartridge (2) and importing hard tube (114) and main cartridge (2) is equipped with sealing ring.
7. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 6, which is characterized in that the main cartridge
(2) and the top ring week of internal box (3) is equipped with abrasionproof bound edge.
8. a kind of device of autonomous acquisition intestinal microecology sample as claimed in claim 7, which is characterized in that the inner cartridge
Distance at the top of body (3) to the distance at the top of main cartridge (2) equal to main cartridge (2) bottom surface to outer box (1) inner bottom surface.
9. such as a kind of application method of the device of autonomous acquisition intestinal microecology sample of any of claims 1-8,
It is characterized in that, specifically including the following steps:
1) outer box bottom is threadedly coupled with toilet seat, sealing cover is opened, keep cotton swab in a vertical state；
2) rotation sliding bar makes cotton swab lodge and pulls out sliding bar after cotton swab is directed at anus by sampling person；
3) sampling person's buttocks pushes, main cartridge opposite outer box body slide downward, at this point, internal box slides up with respect to main cartridge
Dynamic, internal box top surface is broken through sealing film and is flushed to its top surface with main cartridge top surface；
4) normal defecation, excrement are fallen on the sample continuing surface of internal tray interior, reach inspection in the weight of the excrement fallen
Survey is taken, and gravity sensor triggers alarm equipment alarm；
5) then, sampling person opens sealing cover two, squeezes glycerol tube, so that the glycerol in glycerol tube is broken through diaphragm, pass sequentially through and lead
Enter in hard tube and inflow internal box after connection hose and covers fecal sample；
6) it finally, the buttocks of sampling person leaves sample collecting device, can move backward to continue defecation on toilet seat, in defecation
During sliding bar can be resetted to cover sealing cover, outer box removed to complete from toilet seat entirely take
Sample process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910772569.2A CN110448336A (en) | 2019-08-21 | 2019-08-21 | A kind of device and application method of autonomous acquisition intestinal microecology sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910772569.2A CN110448336A (en) | 2019-08-21 | 2019-08-21 | A kind of device and application method of autonomous acquisition intestinal microecology sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110448336A true CN110448336A (en) | 2019-11-15 |
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ID=68488081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910772569.2A Pending CN110448336A (en) | 2019-08-21 | 2019-08-21 | A kind of device and application method of autonomous acquisition intestinal microecology sample |
Country Status (1)
| Country | Link |
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| CN (1) | CN110448336A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112190291A (en) * | 2020-10-13 | 2021-01-08 | 山西农业大学 | Quantitative sampling device for collecting piglet intestinal microecological sample |
-
2019
- 2019-08-21 CN CN201910772569.2A patent/CN110448336A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112190291A (en) * | 2020-10-13 | 2021-01-08 | 山西农业大学 | Quantitative sampling device for collecting piglet intestinal microecological sample |
| CN112190291B (en) * | 2020-10-13 | 2023-06-06 | 山西农业大学 | Quantitative sampling device for collecting intestinal microecological samples of piglets |
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