CN103518783A - Natural bactericide for preventing and controlling ginkgo seedling stem rot and preparation method thereof - Google Patents
Natural bactericide for preventing and controlling ginkgo seedling stem rot and preparation method thereof Download PDFInfo
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- CN103518783A CN103518783A CN201310444777.2A CN201310444777A CN103518783A CN 103518783 A CN103518783 A CN 103518783A CN 201310444777 A CN201310444777 A CN 201310444777A CN 103518783 A CN103518783 A CN 103518783A
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- 235000008100 Ginkgo biloba Nutrition 0.000 title claims abstract description 41
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims description 8
- 235000011201 Ginkgo Nutrition 0.000 title abstract description 9
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 8
- 241000218628 Ginkgo Species 0.000 title 1
- 244000194101 Ginkgo biloba Species 0.000 claims abstract description 40
- 235000015489 Emblica officinalis Nutrition 0.000 claims abstract description 15
- 240000000691 Houttuynia cordata Species 0.000 claims abstract description 15
- 244000277583 Terminalia catappa Species 0.000 claims abstract description 15
- 235000011517 Terminalia chebula Nutrition 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 244000080767 Areca catechu Species 0.000 claims abstract description 10
- 235000006226 Areca catechu Nutrition 0.000 claims abstract description 10
- 241000596154 Belamcanda Species 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 15
- 235000003092 Artemisia dracunculus Nutrition 0.000 claims description 14
- 240000001851 Artemisia dracunculus Species 0.000 claims description 14
- 244000247747 Coptis groenlandica Species 0.000 claims description 14
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 14
- 235000013717 Houttuynia Nutrition 0.000 claims description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 14
- 244000246386 Mentha pulegium Species 0.000 claims description 14
- 235000016257 Mentha pulegium Nutrition 0.000 claims description 14
- 235000004357 Mentha x piperita Nutrition 0.000 claims description 14
- 244000044425 Quisqualis indica Species 0.000 claims description 14
- 235000013399 edible fruits Nutrition 0.000 claims description 14
- 235000001050 hortel pimenta Nutrition 0.000 claims description 14
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 10
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 7
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 7
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 7
- 229910052796 boron Inorganic materials 0.000 claims description 7
- 229910052802 copper Inorganic materials 0.000 claims description 7
- 239000010949 copper Substances 0.000 claims description 7
- 229910052742 iron Inorganic materials 0.000 claims description 7
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 7
- 235000016768 molybdenum Nutrition 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000011701 zinc Substances 0.000 claims description 7
- 229910052725 zinc Inorganic materials 0.000 claims description 7
- -1 30 grams Chemical compound 0.000 claims description 5
- 241000596151 Iris domestica Species 0.000 claims description 5
- 240000008154 Piper betle Species 0.000 claims description 5
- 235000008180 Piper betle Nutrition 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 3
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 239000011733 molybdenum Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 241000037740 Coptis chinensis Species 0.000 abstract 1
- 235000013719 Houttuynia cordata Nutrition 0.000 abstract 1
- 235000006679 Mentha X verticillata Nutrition 0.000 abstract 1
- 235000002899 Mentha suaveolens Nutrition 0.000 abstract 1
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- 239000003905 agrochemical Substances 0.000 description 2
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- 239000003610 charcoal Substances 0.000 description 2
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- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
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- 241000607479 Yersinia pestis Species 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a natural bactericide for preventing and controlling ginkgo seedling stem rot. The natural bactericide is prepared from the following components: radix bupleuri, folium artemisiae argyi, blackberry lily, areca-nut, coptis chinensis, quispualis indica, mint, moutan bark, houttuynia cordata and myrobalan. The natural bactericide for preventing and treating ginkgo seedling stem rot is abundant in raw material source, low in cost, non-toxic and pollution-free, and is prepared from pure natural components; moreover, the ginkgo seedling stem rot can be effectively prevented and controlled.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot and preparation method thereof.
Background technology
Seedlings of Ginkgo biloba stem rot is one of Major Diseases of Seedlings of Ginkgo biloba, in each ginkgo nursery garden, all generally occurs, and the ginkgo that comes across life in 1～2 year nursery stock of growing directly from seeds especially even more serious with annual nursery stock more, often causes seedling mortality.Seedlings of Ginkgo biloba stem rot is seedling base portion browning at the initial stage of a disease, and blade loses normal green, and slightly lets droop, but does not come off.Susceptible position is upwards expansion rapidly, so that complete stool is withered.There is shrinkage in sick seedling base portion cortex, it is spongy or Powdered that intracutaneous tissue rots to be, and look greyish white, and accompany the sclerotium of many tiny black.This germ also can be invaded seedling xylem, thereby the marrow of brown hollow is also shown in bulbil generation sometimes.After this germ extends to root gradually, and root skin cortex is rotted.As with the sick seedling of hand-lifting, can only extract xylem, root cortex stays among soil.Ginkgo cuttage seeding is under the condition of high temperature or low temperature, and stem rot also can occur, and can make cuttings epidermis be tubular and be enclosed within on xylem, and all blackout is rotten for phloem parenchyma.The charcoal rot bacterium that the generation reason of ginkgo seedling stem rot is commonly referred to be Sphaeropsidales, Sphaeropsidaceae, large stem point genus causes, this bacterium happiness high temperature, and suitable growth temperature is 30～32 ℃; And not tight to acid-base value requirement, all can fine growth between pH4～9.
In existing ginkgo seedling raising process, for prevent stem rot normally used be chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticides to cause are on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classifications produce drug resistances, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, to useful biology, can threaten yet.And a lot of chemical pesticides are non-degradables or are difficult to degraded, easily pollute the enrichment of thing at animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", also increase gradually to ecological agricultural chemical demand pollution-free, can natural degradation in market.From the development of pesticide science, on pesticide manufacturing is used, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control Seedlings of Ginkgo biloba stem rot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot provided by the invention, by following component, made: radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia, myrobalan, compound many ore deposits.
Component by following weight portion is made: radix bupleuri 4-6 part, tarragon 4-6 part, blackberry lily 4-6 part, betel nut 4-6 part, coptis 2-4 part, fruit of Rangoon creeper 1-3 part, peppermint 1-3 part, moutan bark 1-3 part, cordate houttuynia 0.5-1.5 part, myrobalan 1-3 part, compound many ore deposits 0.4-0.6 part.
Component by following weight portion is made: 5 parts of radix bupleuri, 5 parts of tarragons, 5 parts of blackberry lilys, 5 parts of betel nuts, 3 parts of the coptiss, 2 parts of the fruits of Rangoon creeper, 2 parts of peppermints, 2 parts of moutan barks, 1 part of cordate houttuynia, 2 parts of myrobalans, 0.5 part, compound many ore deposits.
Wherein, described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, and the compound many ore deposits of per kilogram comprise: 20 grams of boron, 30 grams, zinc, 50 grams of molybdenums, 50 grams, manganese, 20 grams of copper, 100 grams of iron.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control Seedlings of Ginkgo biloba stem rot, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia and myrobalan;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot.
The application of the natural bactericidal agent that the present invention also provides above-mentioned control Seedlings of Ginkgo biloba stem rot in control Seedlings of Ginkgo biloba stem rot, is specially: the natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control Seedlings of Ginkgo biloba stem rot provided by the invention are abundant, with low cost, adopts the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat Seedlings of Ginkgo biloba stem rot.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot, is made by the component of following weight portion: 4 parts of radix bupleuri, 5 parts of tarragons, 4 parts of blackberry lilys, 5 parts of betel nuts, 2 parts of the coptiss, 1 part of the fruit of Rangoon creeper, 3 parts of peppermints, 3 parts of moutan barks, 1.5 parts of cordate houttuynias, 2 parts of myrobalans, 0.5 part, compound many ore deposits.The compound many ore deposits of per kilogram comprise: 20 grams of boron, 30 grams, zinc, 50 grams of molybdenums, 50 grams, manganese, 20 grams of copper, 100 grams of iron.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia and myrobalan;
(2) compound is added to the ethanol water of 8 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot.
Embodiment 2
The natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot, is made by the component of following weight portion: 6 parts of radix bupleuri, 6 parts of tarragons, 6 parts of blackberry lilys, 4 parts of betel nuts, 4 parts of the coptiss, 3 parts of the fruits of Rangoon creeper, 1 part of peppermint, 1 part of moutan bark, 0.5 part of cordate houttuynia, 1 part of myrobalan, 0.4 part, compound many ore deposits.The compound many ore deposits of per kilogram comprise: 20 grams of boron, 30 grams, zinc, 50 grams of molybdenums, 50 grams, manganese, 20 grams of copper, 100 grams of iron.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia and myrobalan;
(2) compound is added to the ethanol water of 5 times of amounts 95%, be heated to 80 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 80 ℃ and decocts 4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot.
Embodiment 3
The natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot, is made by the component of following weight portion: 5 parts of radix bupleuri, 4 parts of tarragons, 5 parts of blackberry lilys, 6 parts of betel nuts, 3 parts of the coptiss, 2 parts of the fruits of Rangoon creeper, 2 parts of peppermints, 2 parts of moutan barks, 1 part of cordate houttuynia, 3 parts of myrobalans, 0.6 part, compound many ore deposits.The compound many ore deposits of per kilogram comprise: 20 grams of boron, 30 grams, zinc, 50 grams of molybdenums, 50 grams, manganese, 20 grams of copper, 100 grams of iron.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia and myrobalan;
(2) compound is added to the ethanol water of 10 times of amounts 95%, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot.
The natural bactericidal agent of the control Seedlings of Ginkgo biloba stem rot of embodiment 4 mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen
This experiment is in vitro, and the natural bactericidal agent of the control Seedlings of Ginkgo biloba stem rot of mensuration embodiment 1 to 3 is active to the inhibition of strawberry pathogen, and method and result are as follows:
1. for examination bacterial classification
(Macrophomina phaszoli (Maubl.) Ashby) is commercially available for charcoal rot bacterium
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method to measure the EC50 value of embodiment of the present invention 1-3 natural bactericidal agent to above-mentioned germ, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the EC50 value for examination pathogen
| Microbial inoculum source | EC50(g/l) |
| Embodiment 1 | 13.5732 |
| Embodiment 2 | 13.7453 |
| Embodiment 3 | 12.4357 |
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3 is tested, diluted concentration is 500 and 1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
The natural bactericidal agent treatment Seedlings of Ginkgo biloba stem rot that adopts embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (6)
1. a natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot, is characterized in that: by following component, made: radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia, myrobalan, compound many ore deposits.
2. a kind of natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot according to claim 1, is characterized in that: the component by following weight portion is made: radix bupleuri 4-6 part, tarragon 4-6 part, blackberry lily 4-6 part, betel nut 4-6 part, coptis 2-4 part, fruit of Rangoon creeper 1-3 part, peppermint 1-3 part, moutan bark 1-3 part, cordate houttuynia 0.5-1.5 part, myrobalan 1-3 part, compound many ore deposits 0.4-0.6 part.
3. a kind of natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot according to claim 1, is characterized in that: the component by following weight portion is made: 5 parts of radix bupleuri, 5 parts of tarragons, 5 parts of blackberry lilys, 5 parts of betel nuts, 3 parts of the coptiss, 2 parts of the fruits of Rangoon creeper, 2 parts of peppermints, 2 parts of moutan barks, 1 part of cordate houttuynia, 2 parts of myrobalans, 0.5 part, compound many ore deposits.
4. a kind of natural bactericidal agent of preventing and treating Seedlings of Ginkgo biloba stem rot according to claim 1, it is characterized in that: described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, the compound many ore deposits of per kilogram comprise: 20 grams of boron, 30 grams, zinc, 50 grams of molybdenums, 50 grams, manganese, 20 grams of copper, 100 grams of iron.
5. a preparation method who prevents and treats the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot, is characterized in that: comprise the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is radix bupleuri, tarragon, blackberry lily, betel nut, the coptis, the fruit of Rangoon creeper, peppermint, moutan bark, cordate houttuynia and myrobalan;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of Seedlings of Ginkgo biloba stem rot.
6. the application of the natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot in control Seedlings of Ginkgo biloba stem rot, is characterized in that: the natural bactericidal agent of control Seedlings of Ginkgo biloba stem rot is mixed and is sterilization microbial inoculum with 500-1000 times of water gaging.
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| CN103828579A (en) * | 2014-02-28 | 2014-06-04 | 唐宝安 | Quick synthesis method of gingko trees |
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| CN105850726A (en) * | 2016-04-08 | 2016-08-17 | 常州润达科技信息咨询有限公司 | Stem rot resistant new gingko variety selecting and breeding method |
| CN105941503A (en) * | 2016-05-25 | 2016-09-21 | 柳州市能兴科技有限公司 | Botanical source fungicide capable of preventing and treating sauropus androgynus stem rot |
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| CN102870833A (en) * | 2012-10-15 | 2013-01-16 | 青岛青大海魁生物技术有限公司 | Chinese medicinal herb marine polypeptide chelate fungicide and preparation method |
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