ZA202103770B - Method for detecting polymerase incorporation of nucleotides - Google Patents
Method for detecting polymerase incorporation of nucleotidesInfo
- Publication number
- ZA202103770B ZA202103770B ZA2021/03770A ZA202103770A ZA202103770B ZA 202103770 B ZA202103770 B ZA 202103770B ZA 2021/03770 A ZA2021/03770 A ZA 2021/03770A ZA 202103770 A ZA202103770 A ZA 202103770A ZA 202103770 B ZA202103770 B ZA 202103770B
- Authority
- ZA
- South Africa
- Prior art keywords
- nucleotides
- polymerase incorporation
- detecting polymerase
- detecting
- incorporation
- Prior art date
Links
- 238000010348 incorporation Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 title 1
- 125000003729 nucleotide group Chemical group 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962890064P | 2019-08-21 | 2019-08-21 | |
| PCT/US2020/046854 WO2021034856A1 (en) | 2019-08-21 | 2020-08-18 | Method for detecting polymerase incorporation of nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA202103770B true ZA202103770B (en) | 2022-10-26 |
Family
ID=74660637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA2021/03770A ZA202103770B (en) | 2019-08-21 | 2021-06-01 | Method for detecting polymerase incorporation of nucleotides |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20220064726A1 (https=) |
| EP (1) | EP4017999A4 (https=) |
| JP (1) | JP7628078B2 (https=) |
| KR (1) | KR20220047205A (https=) |
| CN (1) | CN113286894A (https=) |
| AU (1) | AU2020335007A1 (https=) |
| BR (1) | BR112021012958A2 (https=) |
| CA (1) | CA3123028A1 (https=) |
| IL (1) | IL284065A (https=) |
| MX (1) | MX2021006293A (https=) |
| PH (1) | PH12021551559A1 (https=) |
| TW (1) | TW202120693A (https=) |
| WO (1) | WO2021034856A1 (https=) |
| ZA (1) | ZA202103770B (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102702651B1 (ko) * | 2021-12-10 | 2024-09-04 | 서울시립대학교 산학협력단 | Rna 중합효소 탐지를 위한 핵산기반 막구조체 |
| WO2024077047A1 (en) * | 2022-10-05 | 2024-04-11 | Esbiolab Llc | Methods and compositions for substrate surface chemistry |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821058A (en) * | 1984-01-16 | 1998-10-13 | California Institute Of Technology | Automated DNA sequencing technique |
| FR2726286B1 (fr) * | 1994-10-28 | 1997-01-17 | Genset Sa | Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede |
| US7138254B2 (en) * | 1999-08-02 | 2006-11-21 | Ge Healthcare (Sv) Corp. | Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins |
| US6824980B2 (en) * | 2000-06-08 | 2004-11-30 | Xiao Bing Wang | Isometric primer extension method and kit for detection and quantification of specific nucleic acid |
| US6861214B1 (en) * | 2000-10-23 | 2005-03-01 | Beckman Coulter, Inc. | Immobilization of biopolymers to aminated substrates by direct adsorption |
| JP2004523243A (ja) * | 2001-03-12 | 2004-08-05 | カリフォルニア インスティチュート オブ テクノロジー | 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置 |
| US20100190151A1 (en) * | 2003-06-10 | 2010-07-29 | Quake Stephen R | Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids |
| SG169356A1 (en) * | 2006-02-08 | 2011-03-30 | Illumina Cambridge Ltd | Method for sequencing a polynucleotide template |
| JP2009124957A (ja) * | 2007-11-20 | 2009-06-11 | Sumitomo Bakelite Co Ltd | マイクロｒｎａの検出方法、これに用いられるマイクロアレイおよびキット |
| WO2013019361A1 (en) * | 2011-07-07 | 2013-02-07 | Life Technologies Corporation | Sequencing methods |
| US9012022B2 (en) * | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| PT3218511T (pt) * | 2014-11-11 | 2020-07-23 | Illumina Cambridge Ltd | Métodos e dispositivos para produção e sequenciamento de grupos monoclonais de ácido nucleico |
| US10955332B2 (en) * | 2016-12-22 | 2021-03-23 | Illumina, Inc. | Flow cell package and method for making the same |
-
2020
- 2020-08-13 TW TW109127578A patent/TW202120693A/zh unknown
- 2020-08-18 AU AU2020335007A patent/AU2020335007A1/en not_active Abandoned
- 2020-08-18 PH PH1/2021/551559A patent/PH12021551559A1/en unknown
- 2020-08-18 KR KR1020217019686A patent/KR20220047205A/ko active Pending
- 2020-08-18 JP JP2021537767A patent/JP7628078B2/ja active Active
- 2020-08-18 CN CN202080007619.9A patent/CN113286894A/zh active Pending
- 2020-08-18 EP EP20855613.4A patent/EP4017999A4/en not_active Withdrawn
- 2020-08-18 US US17/418,298 patent/US20220064726A1/en not_active Abandoned
- 2020-08-18 CA CA3123028A patent/CA3123028A1/en active Pending
- 2020-08-18 BR BR112021012958A patent/BR112021012958A2/pt not_active IP Right Cessation
- 2020-08-18 WO PCT/US2020/046854 patent/WO2021034856A1/en not_active Ceased
- 2020-08-18 MX MX2021006293A patent/MX2021006293A/es unknown
-
2021
- 2021-06-01 ZA ZA2021/03770A patent/ZA202103770B/en unknown
- 2021-06-16 IL IL284065A patent/IL284065A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX2021006293A (es) | 2021-08-24 |
| CN113286894A (zh) | 2021-08-20 |
| JP7628078B2 (ja) | 2025-02-07 |
| KR20220047205A (ko) | 2022-04-15 |
| WO2021034856A1 (en) | 2021-02-25 |
| AU2020335007A1 (en) | 2021-06-17 |
| US20220064726A1 (en) | 2022-03-03 |
| EP4017999A4 (en) | 2023-10-04 |
| BR112021012958A2 (pt) | 2022-03-03 |
| PH12021551559A1 (en) | 2022-04-11 |
| TW202120693A (zh) | 2021-06-01 |
| CA3123028A1 (en) | 2021-02-25 |
| IL284065A (en) | 2021-08-31 |
| JP2022545307A (ja) | 2022-10-27 |
| EP4017999A1 (en) | 2022-06-29 |
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