CN113286894A - 用于检测通过聚合酶而掺入核苷酸的方法 - Google Patents

用于检测通过聚合酶而掺入核苷酸的方法 Download PDF

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Publication number
CN113286894A
CN113286894A CN202080007619.9A CN202080007619A CN113286894A CN 113286894 A CN113286894 A CN 113286894A CN 202080007619 A CN202080007619 A CN 202080007619A CN 113286894 A CN113286894 A CN 113286894A
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primer
immobilized
test
primers
fluorescent
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CN202080007619.9A
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English (en)
Chinese (zh)
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L·文森特
A·普拉布
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Illumina Inc
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Illumina Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080007619.9A 2019-08-21 2020-08-18 用于检测通过聚合酶而掺入核苷酸的方法 Pending CN113286894A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962890064P 2019-08-21 2019-08-21
US62/890,064 2019-08-21
PCT/US2020/046854 WO2021034856A1 (en) 2019-08-21 2020-08-18 Method for detecting polymerase incorporation of nucleotides

Publications (1)

Publication Number Publication Date
CN113286894A true CN113286894A (zh) 2021-08-20

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CN202080007619.9A Pending CN113286894A (zh) 2019-08-21 2020-08-18 用于检测通过聚合酶而掺入核苷酸的方法

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Country Link
US (1) US20220064726A1 (https=)
EP (1) EP4017999A4 (https=)
JP (1) JP7628078B2 (https=)
KR (1) KR20220047205A (https=)
CN (1) CN113286894A (https=)
AU (1) AU2020335007A1 (https=)
BR (1) BR112021012958A2 (https=)
CA (1) CA3123028A1 (https=)
IL (1) IL284065A (https=)
MX (1) MX2021006293A (https=)
PH (1) PH12021551559A1 (https=)
TW (1) TW202120693A (https=)
WO (1) WO2021034856A1 (https=)
ZA (1) ZA202103770B (https=)

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Publication number Priority date Publication date Assignee Title
KR102702651B1 (ko) * 2021-12-10 2024-09-04 서울시립대학교 산학협력단 Rna 중합효소 탐지를 위한 핵산기반 막구조체
WO2024077047A1 (en) * 2022-10-05 2024-04-11 Esbiolab Llc Methods and compositions for substrate surface chemistry

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274351B1 (en) * 1994-10-28 2001-08-14 Genset Solid support for solid phase amplification and sequencing and method for preparing the same nucleic acid
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
WO2007091077A1 (en) * 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
US20100190151A1 (en) * 2003-06-10 2010-07-29 Quake Stephen R Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
WO2016075204A1 (en) * 2014-11-11 2016-05-19 Illumina, Inc. Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
US20180195950A1 (en) * 2016-12-22 2018-07-12 Illumina, Inc. Flow cell package and method for making the same

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US7138254B2 (en) * 1999-08-02 2006-11-21 Ge Healthcare (Sv) Corp. Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
US6861214B1 (en) * 2000-10-23 2005-03-01 Beckman Coulter, Inc. Immobilization of biopolymers to aminated substrates by direct adsorption
JP2009124957A (ja) * 2007-11-20 2009-06-11 Sumitomo Bakelite Co Ltd マイクロｒｎａの検出方法、これに用いられるマイクロアレイおよびキット
WO2013019361A1 (en) * 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274351B1 (en) * 1994-10-28 2001-08-14 Genset Solid support for solid phase amplification and sequencing and method for preparing the same nucleic acid
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20100190151A1 (en) * 2003-06-10 2010-07-29 Quake Stephen R Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
WO2007091077A1 (en) * 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
WO2016075204A1 (en) * 2014-11-11 2016-05-19 Illumina, Inc. Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
US20180195950A1 (en) * 2016-12-22 2018-07-12 Illumina, Inc. Flow cell package and method for making the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KENJI KINOSHITA ET AL.,: ""Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer"", 《NUCLEIC ACIDS RES》, vol. 35, no. 1, pages 3 - 1 *
MICHAEL C. PIRRUNG ET AL.,: ""The Arrayed Primer Extension Method for DNA Microchip Analysis. Molecular Computation of Satisfaction Problems"", 《J. AM. CHEM. SOC》, vol. 122, no. 9, pages 1873 - 1882, XP093076092, DOI: 10.1021/ja992392j *

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Publication number Publication date
MX2021006293A (es) 2021-08-24
JP7628078B2 (ja) 2025-02-07
KR20220047205A (ko) 2022-04-15
WO2021034856A1 (en) 2021-02-25
AU2020335007A1 (en) 2021-06-17
US20220064726A1 (en) 2022-03-03
EP4017999A4 (en) 2023-10-04
BR112021012958A2 (pt) 2022-03-03
ZA202103770B (en) 2022-10-26
PH12021551559A1 (en) 2022-04-11
TW202120693A (zh) 2021-06-01
CA3123028A1 (en) 2021-02-25
IL284065A (en) 2021-08-31
JP2022545307A (ja) 2022-10-27
EP4017999A1 (en) 2022-06-29

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Application publication date: 20210820