JP7628078B2 - ヌクレオチドのポリメラーゼ組み込みを検出する方法 - Google Patents

ヌクレオチドのポリメラーゼ組み込みを検出する方法 Download PDF

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JP7628078B2
JP7628078B2 JP2021537767A JP2021537767A JP7628078B2 JP 7628078 B2 JP7628078 B2 JP 7628078B2 JP 2021537767 A JP2021537767 A JP 2021537767A JP 2021537767 A JP2021537767 A JP 2021537767A JP 7628078 B2 JP7628078 B2 JP 7628078B2
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primer
immobilized
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primers
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ビンセント，ルドビック
プラブ，アンミヴ
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イルミナ インコーポレイテッド
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021537767A 2019-08-21 2020-08-18 ヌクレオチドのポリメラーゼ組み込みを検出する方法 Active JP7628078B2 (ja)

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US201962890064P 2019-08-21 2019-08-21
US62/890,064 2019-08-21
PCT/US2020/046854 WO2021034856A1 (en) 2019-08-21 2020-08-18 Method for detecting polymerase incorporation of nucleotides

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JP2022545307A JP2022545307A (ja) 2022-10-27
JP2022545307A5 JP2022545307A5 (https=) 2023-08-10
JP7628078B2 true JP7628078B2 (ja) 2025-02-07

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US (1) US20220064726A1 (https=)
EP (1) EP4017999A4 (https=)
JP (1) JP7628078B2 (https=)
KR (1) KR20220047205A (https=)
CN (1) CN113286894A (https=)
AU (1) AU2020335007A1 (https=)
BR (1) BR112021012958A2 (https=)
CA (1) CA3123028A1 (https=)
IL (1) IL284065A (https=)
MX (1) MX2021006293A (https=)
PH (1) PH12021551559A1 (https=)
TW (1) TW202120693A (https=)
WO (1) WO2021034856A1 (https=)
ZA (1) ZA202103770B (https=)

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KR102702651B1 (ko) * 2021-12-10 2024-09-04 서울시립대학교 산학협력단 Rna 중합효소 탐지를 위한 핵산기반 막구조체
WO2024077047A1 (en) * 2022-10-05 2024-04-11 Esbiolab Llc Methods and compositions for substrate surface chemistry

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
JP2009124957A (ja) 2007-11-20 2009-06-11 Sumitomo Bakelite Co Ltd マイクロｒｎａの検出方法、これに用いられるマイクロアレイおよびキット
JP2017533710A (ja) 2014-11-11 2017-11-16 イルミナ ケンブリッジ リミテッド 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

Family Cites Families (10)

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US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
FR2726286B1 (fr) * 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US7138254B2 (en) * 1999-08-02 2006-11-21 Ge Healthcare (Sv) Corp. Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
US6861214B1 (en) * 2000-10-23 2005-03-01 Beckman Coulter, Inc. Immobilization of biopolymers to aminated substrates by direct adsorption
US20100190151A1 (en) * 2003-06-10 2010-07-29 Quake Stephen R Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
SG169356A1 (en) * 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
WO2013019361A1 (en) * 2011-07-07 2013-02-07 Life Technologies Corporation Sequencing methods
US9012022B2 (en) * 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US10955332B2 (en) * 2016-12-22 2021-03-23 Illumina, Inc. Flow cell package and method for making the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
JP2009124957A (ja) 2007-11-20 2009-06-11 Sumitomo Bakelite Co Ltd マイクロｒｎａの検出方法、これに用いられるマイクロアレイおよびキット
JP2017533710A (ja) 2014-11-11 2017-11-16 イルミナ ケンブリッジ リミテッド 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ

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MX2021006293A (es) 2021-08-24
CN113286894A (zh) 2021-08-20
KR20220047205A (ko) 2022-04-15
WO2021034856A1 (en) 2021-02-25
AU2020335007A1 (en) 2021-06-17
US20220064726A1 (en) 2022-03-03
EP4017999A4 (en) 2023-10-04
BR112021012958A2 (pt) 2022-03-03
ZA202103770B (en) 2022-10-26
PH12021551559A1 (en) 2022-04-11
TW202120693A (zh) 2021-06-01
CA3123028A1 (en) 2021-02-25
IL284065A (en) 2021-08-31
JP2022545307A (ja) 2022-10-27
EP4017999A1 (en) 2022-06-29

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