ZA200403434B - Genetic vaccine against human immunodeficiency virus - Google Patents
Genetic vaccine against human immunodeficiency virus Download PDFInfo
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- ZA200403434B ZA200403434B ZA200403434A ZA200403434A ZA200403434B ZA 200403434 B ZA200403434 B ZA 200403434B ZA 200403434 A ZA200403434 A ZA 200403434A ZA 200403434 A ZA200403434 A ZA 200403434A ZA 200403434 B ZA200403434 B ZA 200403434B
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- hiv
- antigen
- recombinant adenovirus
- virus
- recombinant
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| US6733993B2 (en) | 2000-09-15 | 2004-05-11 | Merck & Co., Inc. | Enhanced first generation adenovirus vaccines expressing codon optimized HIV1-gag, pol, nef and modifications |
| US20040101957A1 (en) * | 2001-09-14 | 2004-05-27 | Emini Emilio A. | Enhanced first generation adenovirus vaccines expressing codon optimized hiv1-gag, pol.nef and modifications |
| WO2005012538A2 (en) | 2003-08-01 | 2005-02-10 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Accelerated vaccination |
| GB0417494D0 (en) * | 2004-08-05 | 2004-09-08 | Glaxosmithkline Biolog Sa | Vaccine |
| WO2006039045A2 (en) * | 2004-09-01 | 2006-04-13 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Adenoviral vectors able to transduce apcs, potential use in immune response generation |
| WO2006050394A2 (en) * | 2004-11-01 | 2006-05-11 | Novartis Vaccines And Diagnostics Inc. | Combination approaches for generating immune responses |
| SG159555A1 (en) | 2004-11-16 | 2010-03-30 | Crucell Holland Bv | Multivalent vaccines comprising recombinant viral vectors |
| US8450055B2 (en) * | 2005-08-31 | 2013-05-28 | The United States Of America As Represented By The Secretary Of The Navy | Malaria antigen screening method |
| AU2006284756B2 (en) * | 2005-08-31 | 2012-06-07 | Genvec, Inc. | Adenoviral vector-based malaria vaccines |
| KR101441843B1 (ko) * | 2005-10-18 | 2014-09-17 | 내셔날 쥬이쉬 헬스 | 조건부 불멸화 장기 줄기 세포 및 상기 세포의 제조 및 사용 방법 |
| WO2007055952A2 (en) * | 2005-11-03 | 2007-05-18 | Wyeth | Process for producing stable hiv th-ctl peptides |
| DK1996238T3 (da) | 2006-02-28 | 2016-08-01 | Vaxart Inc | Kimæriske adenovirale vektorer og DsRNA som TLR3-agonist |
| WO2007134325A2 (en) * | 2006-05-15 | 2007-11-22 | Introgen Therapeutics, Inc. | Methods and compositions for protein production using adenoviral vectors |
| WO2008086386A2 (en) * | 2007-01-09 | 2008-07-17 | Genvec, Inc. | Adenoviral vector-based malaria vaccines |
| AU2008352966C1 (en) * | 2007-12-18 | 2014-09-25 | Boston Medical Center Corporation | Pre- or post-exposure treatment for filovirus or arenavirus infection |
| US20110217328A1 (en) * | 2007-12-18 | 2011-09-08 | Trustees Of Boston University | Compositions and methods for treating ebola virus infection |
| WO2009111738A2 (en) * | 2008-03-06 | 2009-09-11 | Mayo Foundation For Medical Education And Research | Single cycle replicating adenovirus vectors |
| WO2009120306A1 (en) * | 2008-03-25 | 2009-10-01 | Trustees Of Boston University | Multivalent vaccine vector for the treatment and inhibition of viral infection |
| US10071154B2 (en) | 2008-04-04 | 2018-09-11 | The Trustees Of The University Of Pennsylvania | Vaccines and immunotherapeutics using IL-28 and compositions and methods of using the same |
| EP2285832B1 (en) | 2008-05-16 | 2020-08-26 | Taiga Biotechnologies, Inc. | Antibodies and processes for preparing the same |
| CN101591379B (zh) | 2008-05-27 | 2017-01-18 | 中国疾病预防控制中心性病艾滋病预防控制中心 | 基于eiav减毒活疫苗的氨基酸突变而构建的抗hiv疫苗 |
| ES2525411T3 (es) | 2008-07-21 | 2014-12-22 | Taiga Biotechnologies, Inc. | Células anucleadas diferenciadas y método para preparar las mismas |
| EP2318435B1 (en) | 2008-08-28 | 2015-12-23 | Taiga Biotechnologies, Inc. | Modulators of myc, methods of using the same, and methods of identifying agents that modulate myc |
| EP3101122B1 (en) | 2009-08-24 | 2023-06-14 | Baylor College of Medicine | Generation of ctl lines with specificity against multiple tumor antigens or multiple viruses |
| EP2603799A4 (en) | 2010-08-12 | 2014-02-12 | Clontech Lab Inc | SIDEFLOW ASSAYS FOR NON-DIAGNOSTIC ANALYSIS |
| AP2013007166A0 (en) | 2011-04-06 | 2013-10-31 | Univ Paris Descartes Inst De Rech Pour Le Dev Ird | Pharmaceutical compositions for preventing and/or treating an HIV disease in humans |
| GB201121308D0 (en) | 2011-12-12 | 2012-01-25 | Cell Medica Ltd | Process |
| CA2861335A1 (en) * | 2012-01-26 | 2013-08-01 | Ibc Pharmaceuticals, Inc. | Targeting interferon-lambda with antibodies potently enhances anti-tumor and anti-viral activities |
| PT2812431T (pt) | 2012-02-09 | 2019-10-18 | Baylor College Medicine | Pepmixes para gerar ctls multivirais com larga especifidade |
| JP6285930B2 (ja) | 2012-07-20 | 2018-02-28 | タイガ バイオテクノロジーズ，インク． | 造血コンパートメントの再構築及び自家再構築の増進 |
| CN104995299B (zh) * | 2012-12-11 | 2020-02-28 | 宝生物工程株式会社 | 表达盒 |
| GB201301119D0 (en) * | 2013-01-22 | 2013-03-06 | Vaxxit Srl | Viral vaccines |
| US10272115B2 (en) | 2013-03-11 | 2019-04-30 | Taiga Biotechnologies, Inc. | Production and use of red blood cells |
| US9365825B2 (en) | 2013-03-11 | 2016-06-14 | Taiga Biotechnologies, Inc. | Expansion of adult stem cells in vitro |
| AU2014359085B2 (en) * | 2013-12-08 | 2017-11-02 | Peptcell Limited | HIV antigens and antibodies and compositions, methods and uses thereof |
| JP6999941B2 (ja) | 2015-09-18 | 2022-02-10 | ベイラー カレッジ オブ メディスン | 病原体からの免疫原性抗原同定および臨床的有効性との相関 |
| TWI746473B (zh) * | 2015-11-02 | 2021-11-21 | 美商辛分子醫藥有限公司 | 針對細胞內抗原之單域抗體 |
| CN107149675A (zh) * | 2016-03-10 | 2017-09-12 | 广东思峰生物科技有限责任公司 | 一种白细胞介素-12的新用途 |
| KR20190092472A (ko) | 2016-12-02 | 2019-08-07 | 타이가 바이오테크놀로지스, 인코포레이티드 | 나노입자 제제 |
| US10149898B2 (en) | 2017-08-03 | 2018-12-11 | Taiga Biotechnologies, Inc. | Methods and compositions for the treatment of melanoma |
| CN108823232A (zh) * | 2018-04-20 | 2018-11-16 | 中国科学院广州生物医药与健康研究院 | 一种艾滋病疫苗及其制备方法 |
| RU2722648C2 (ru) * | 2018-11-16 | 2020-06-02 | федеральное государственное бюджетное учреждение "Национальный исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации | Способ проверки иммуногенности вакцинных антигенов для получения высокоэффективных вакцин против опасных инфекций |
| EP4582440A3 (en) * | 2018-12-21 | 2025-10-08 | The Regents of the University of California | Il-10-containing vaccines and uses thereof |
| WO2020210231A1 (en) | 2019-04-08 | 2020-10-15 | Taiga Biotechnologies, Inc. | Compositions and methods for the cry opreservation of immune cells |
| EP3969041A4 (en) | 2019-05-14 | 2023-05-10 | Taiga Biotechnologies, Inc. | COMPOSITIONS AND METHODS FOR TREATMENT OF T-CELL DEPLETION |
| CN111117974B (zh) * | 2019-12-20 | 2022-02-22 | 华南农业大学 | 一种可视化绿色荧光猪伪狂犬病毒及其构建方法 |
| WO2024048793A1 (ja) * | 2022-09-02 | 2024-03-07 | 国立研究開発法人医薬基盤・健康・栄養研究所 | 感染を治療するための弱毒化ウイルス |
| CN119390828A (zh) * | 2024-12-31 | 2025-02-07 | 天津龙晟生物科技有限公司 | 人类免疫缺陷病毒hiv抗体及其应用和其检测产品 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5866136A (en) * | 1986-08-01 | 1999-02-02 | Commonwealth Scientific And Industrial Organisation | Recombinant vaccine |
| US5141867A (en) * | 1987-02-02 | 1992-08-25 | E. I. Du Pont De Nemours And Company | Nucleotide sequence encoding a human immunodeficiency virus antigen |
| US5516657A (en) * | 1992-05-11 | 1996-05-14 | Cambridge Biotech Corporation | Baculovirus vectors for expression of secretory and membrane-bound proteins |
| CA2201592A1 (en) * | 1994-10-03 | 1996-04-18 | The Government Of The United States Of America, Represented By The Secre Tary, Department Of Health And Human Services | Enhanced immune response by introduction of cytokine gene and/or costimulatory molecule b7 gene in a recombinant virus expressing system |
| AU2678797A (en) * | 1996-04-22 | 1997-11-12 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Heterologous boosting immunizations |
| CA2378539A1 (en) * | 1999-07-06 | 2001-01-11 | Merck & Co., Inc. | Adenovirus carrying gag gene hiv vaccine |
| US6544780B1 (en) * | 2000-06-02 | 2003-04-08 | Genphar, Inc. | Adenovirus vector with multiple expression cassettes |
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- 2002-11-01 KR KR1020047006676A patent/KR20050042458A/ko not_active Abandoned
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- 2002-11-01 EP EP02784374A patent/EP1451329A4/en not_active Ceased
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| US20030219458A1 (en) | 2003-11-27 |
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| EP1451329A2 (en) | 2004-09-01 |
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| HK1077601A1 (zh) | 2006-02-17 |
| US20020155127A1 (en) | 2002-10-24 |
| WO2003038057A2 (en) | 2003-05-08 |
| KR20050042458A (ko) | 2005-05-09 |
| CA2465037A1 (en) | 2003-05-08 |
| CN1636063A (zh) | 2005-07-06 |
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