ZA200103145B - Pesticidal proteins. - Google Patents
Pesticidal proteins. Download PDFInfo
- Publication number
- ZA200103145B ZA200103145B ZA200103145A ZA200103145A ZA200103145B ZA 200103145 B ZA200103145 B ZA 200103145B ZA 200103145 A ZA200103145 A ZA 200103145A ZA 200103145 A ZA200103145 A ZA 200103145A ZA 200103145 B ZA200103145 B ZA 200103145B
- Authority
- ZA
- South Africa
- Prior art keywords
- seq
- kda
- protein
- toxin
- dna
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 295
- 102000004169 proteins and genes Human genes 0.000 title claims description 151
- 230000000361 pesticidal effect Effects 0.000 title claims description 60
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 77
- 241000196324 Embryophyta Species 0.000 claims description 61
- 238000000034 method Methods 0.000 claims description 53
- 241000607479 Yersinia pestis Species 0.000 claims description 49
- 239000002773 nucleotide Substances 0.000 claims description 49
- 125000003729 nucleotide group Chemical group 0.000 claims description 49
- 108091033319 polynucleotide Proteins 0.000 claims description 45
- 239000002157 polynucleotide Substances 0.000 claims description 45
- 102000040430 polynucleotide Human genes 0.000 claims description 45
- 240000008042 Zea mays Species 0.000 claims description 31
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 26
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 21
- 150000001413 amino acids Chemical class 0.000 claims description 21
- 235000005822 corn Nutrition 0.000 claims description 21
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 18
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 15
- 230000000295 complement effect Effects 0.000 claims description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 9
- 241000489947 Diabrotica virgifera virgifera Species 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 8
- 230000009261 transgenic effect Effects 0.000 claims description 7
- 102100038968 WAP four-disulfide core domain protein 1 Human genes 0.000 claims description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 2
- 229940127593 SEQ-9 Drugs 0.000 claims 2
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 229940070376 protein Drugs 0.000 claims 1
- 108700012359 toxins Proteins 0.000 description 299
- 239000003053 toxin Substances 0.000 description 238
- 231100000765 toxin Toxicity 0.000 description 238
- 108020004414 DNA Proteins 0.000 description 97
- 108091028043 Nucleic acid sequence Proteins 0.000 description 81
- 101710164418 Movement protein TGB2 Proteins 0.000 description 80
- 102100021225 Serine hydroxymethyltransferase, cytosolic Human genes 0.000 description 74
- 210000004027 cell Anatomy 0.000 description 72
- 239000013615 primer Substances 0.000 description 68
- 239000002987 primer (paints) Substances 0.000 description 68
- 239000012634 fragment Substances 0.000 description 52
- 108090000765 processed proteins & peptides Proteins 0.000 description 51
- 239000000523 sample Substances 0.000 description 36
- 101800001509 Large capsid protein Proteins 0.000 description 32
- 239000008188 pellet Substances 0.000 description 32
- 230000036961 partial effect Effects 0.000 description 30
- 239000013612 plasmid Substances 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 24
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 23
- 241000238631 Hexapoda Species 0.000 description 22
- 108700026244 Open Reading Frames Proteins 0.000 description 22
- 239000000872 buffer Substances 0.000 description 22
- 239000012528 membrane Substances 0.000 description 22
- 102000004196 processed proteins & peptides Human genes 0.000 description 22
- 241000588724 Escherichia coli Species 0.000 description 21
- 238000004166 bioassay Methods 0.000 description 21
- 230000000694 effects Effects 0.000 description 21
- 238000009396 hybridization Methods 0.000 description 21
- 230000002441 reversible effect Effects 0.000 description 21
- 239000013078 crystal Substances 0.000 description 19
- 239000000203 mixture Substances 0.000 description 19
- 229920001184 polypeptide Polymers 0.000 description 19
- 108091034117 Oligonucleotide Proteins 0.000 description 18
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 18
- 238000011282 treatment Methods 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 17
- 239000000499 gel Substances 0.000 description 16
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 239000013598 vector Substances 0.000 description 15
- 241000193386 Lysinibacillus sphaericus Species 0.000 description 14
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 14
- 239000002751 oligonucleotide probe Substances 0.000 description 14
- 239000000575 pesticide Substances 0.000 description 14
- 239000000243 solution Substances 0.000 description 14
- 238000010561 standard procedure Methods 0.000 description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 13
- 239000000725 suspension Substances 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 238000002105 Southern blotting Methods 0.000 description 12
- 239000002158 endotoxin Substances 0.000 description 12
- 239000000284 extract Substances 0.000 description 12
- 230000009466 transformation Effects 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 11
- 230000004927 fusion Effects 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 238000005119 centrifugation Methods 0.000 description 10
- 238000009472 formulation Methods 0.000 description 10
- 239000000843 powder Substances 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- 230000002588 toxic effect Effects 0.000 description 10
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 9
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 9
- 238000010367 cloning Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- 239000001509 sodium citrate Substances 0.000 description 9
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 231100000331 toxic Toxicity 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 241000364057 Peoria Species 0.000 description 8
- 238000000502 dialysis Methods 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 8
- 230000037213 diet Effects 0.000 description 8
- 230000002068 genetic effect Effects 0.000 description 8
- 230000009036 growth inhibition Effects 0.000 description 8
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 8
- 238000006467 substitution reaction Methods 0.000 description 8
- 101001057129 Bacillus cereus Enterotoxin Proteins 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- 101000700655 Mycobacterium leprae (strain TN) Serine-rich antigen Proteins 0.000 description 7
- 241000589540 Pseudomonas fluorescens Species 0.000 description 7
- 238000012300 Sequence Analysis Methods 0.000 description 7
- 108700019146 Transgenes Proteins 0.000 description 7
- 241000700605 Viruses Species 0.000 description 7
- 238000013461 design Methods 0.000 description 7
- 238000004520 electroporation Methods 0.000 description 7
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 239000006166 lysate Substances 0.000 description 7
- 241000894007 species Species 0.000 description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 6
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 108091092356 cellular DNA Proteins 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 6
- 231100000673 dose–response relationship Toxicity 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000000749 insecticidal effect Effects 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 108091026890 Coding region Proteins 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 5
- 241000254173 Coleoptera Species 0.000 description 5
- 101710151559 Crystal protein Proteins 0.000 description 5
- 241000255925 Diptera Species 0.000 description 5
- 241001508566 Hypera postica Species 0.000 description 5
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 5
- 230000004071 biological effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 235000009973 maize Nutrition 0.000 description 5
- 238000010369 molecular cloning Methods 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 239000013605 shuttle vector Substances 0.000 description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 108700010070 Codon Usage Proteins 0.000 description 4
- 230000007023 DNA restriction-modification system Effects 0.000 description 4
- 241000489976 Diabrotica undecimpunctata howardi Species 0.000 description 4
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 239000002033 PVDF binder Substances 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 108091081024 Start codon Proteins 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 4
- 230000002363 herbicidal effect Effects 0.000 description 4
- 239000004009 herbicide Substances 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000002917 insecticide Substances 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- -1 lle Chemical group 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 101001113903 Grapevine leafroll-associated virus 3 (isolate United States/NY1) Protein P4 Proteins 0.000 description 3
- 241001508570 Hypera brunneipennis Species 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 3
- 241000215495 Massilia timonae Species 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 108091093037 Peptide nucleic acid Proteins 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 108020005038 Terminator Codon Proteins 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000001588 bifunctional effect Effects 0.000 description 3
- 229960003669 carbenicillin Drugs 0.000 description 3
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 229940104302 cytosine Drugs 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000000326 densiometry Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Substances NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 3
- 238000002523 gelfiltration Methods 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 210000003000 inclusion body Anatomy 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 3
- 108010052968 leupeptin Proteins 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 229950000964 pepstatin Drugs 0.000 description 3
- 108010091212 pepstatin Proteins 0.000 description 3
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 3
- 230000036515 potency Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 230000007888 toxin activity Effects 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 101800003405 15 kDa peptide Proteins 0.000 description 2
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 2
- 101710092702 47 kDa protein Proteins 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 2
- 241000588986 Alcaligenes Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000223651 Aureobasidium Species 0.000 description 2
- 241000589151 Azotobacter Species 0.000 description 2
- 241001147758 Bacillus thuringiensis serovar kurstaki Species 0.000 description 2
- 101000664393 Bacillus thuringiensis subsp. finitimus S-layer protein Proteins 0.000 description 2
- VGGGPCQERPFHOB-MCIONIFRSA-N Bestatin Chemical compound CC(C)C[C@H](C(O)=O)NC(=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1 VGGGPCQERPFHOB-MCIONIFRSA-N 0.000 description 2
- VGGGPCQERPFHOB-UHFFFAOYSA-N Bestatin Natural products CC(C)CC(C(O)=O)NC(=O)C(O)C(N)CC1=CC=CC=C1 VGGGPCQERPFHOB-UHFFFAOYSA-N 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 241000256054 Culex <genus> Species 0.000 description 2
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 2
- 239000003298 DNA probe Substances 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 241000489975 Diabrotica Species 0.000 description 2
- 241000489972 Diabrotica barberi Species 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229930010555 Inosine Natural products 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- 241000258916 Leptinotarsa decemlineata Species 0.000 description 2
- 241000219823 Medicago Species 0.000 description 2
- 108050004278 Mosquitocidal toxin Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 108020005120 Plant DNA Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 241000589180 Rhizobium Species 0.000 description 2
- 241000220317 Rosa Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 101000980867 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Curved DNA-binding protein Proteins 0.000 description 2
- 101000982319 Shallot virus X Uncharacterized ORF4 protein Proteins 0.000 description 2
- 208000034972 Sudden Infant Death Diseases 0.000 description 2
- 206010042440 Sudden infant death syndrome Diseases 0.000 description 2
- 108700005078 Synthetic Genes Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000003139 biocide Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 229960003276 erythromycin Drugs 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 230000000974 larvacidal effect Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000012669 liquid formulation Substances 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002231 mosquitocidal effect Effects 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000011236 particulate material Substances 0.000 description 2
- 101150113864 pat gene Proteins 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Chemical compound O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 231100000654 protein toxin Toxicity 0.000 description 2
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 101150038671 strat gene Proteins 0.000 description 2
- 101150012509 sub gene Proteins 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 229950009811 ubenimex Drugs 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 101710134681 40 kDa protein Proteins 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 244000235858 Acetobacter xylinum Species 0.000 description 1
- 235000002837 Acetobacter xylinum Nutrition 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000902874 Agelastica alni Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 241000180579 Arca Species 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000193365 Bacillus thuringiensis serovar israelensis Species 0.000 description 1
- 241000193369 Bacillus thuringiensis serovar tenebrionis Species 0.000 description 1
- 101100275988 Bacillus thuringiensis subsp. israelensis cyt2Ba1 gene Proteins 0.000 description 1
- 101100007933 Bacillus thuringiensis subsp. kyushuensis cyt2Aa1 gene Proteins 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 101000653197 Beet necrotic yellow vein virus (isolate Japan/S) Movement protein TGB3 Proteins 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 239000011547 Bouin solution Substances 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 101150001086 COB gene Proteins 0.000 description 1
- 241000905957 Channa melasoma Species 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- CHBRHODLKOZEPZ-UHFFFAOYSA-N Clotiazepam Chemical compound S1C(CC)=CC2=C1N(C)C(=O)CN=C2C1=CC=CC=C1Cl CHBRHODLKOZEPZ-UHFFFAOYSA-N 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 101100139611 Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis) qcrB gene Proteins 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 241000254171 Curculionidae Species 0.000 description 1
- 241001071944 Cyta Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- IDBRULKICVRMNG-VKHMYHEASA-N Demethylphosphinothricin Chemical compound OC(=O)[C@@H](N)CCP(O)=O IDBRULKICVRMNG-VKHMYHEASA-N 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 244000228957 Ferula foetida Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000190714 Gymnosporangium clavipes Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000012741 Laemmli sample buffer Substances 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 238000003231 Lowry assay Methods 0.000 description 1
- 238000009013 Lowry's assay Methods 0.000 description 1
- 241001625930 Luria Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241001443590 Naganishia albida Species 0.000 description 1
- 241000033319 Naganishia diffluens Species 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 241001585676 Orthonama obstipata Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001147397 Ostrinia Species 0.000 description 1
- 241000346285 Ostrinia furnacalis Species 0.000 description 1
- 241001147398 Ostrinia nubilalis Species 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000222051 Papiliotrema laurentii Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 101000798429 Pinus strobus Putative 2-Cys peroxiredoxin BAS1 Proteins 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 238000012181 QIAquick gel extraction kit Methods 0.000 description 1
- 101100173636 Rattus norvegicus Fhl2 gene Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000191043 Rhodobacter sphaeroides Species 0.000 description 1
- 241000223252 Rhodotorula Species 0.000 description 1
- 241000223253 Rhodotorula glutinis Species 0.000 description 1
- 241000223254 Rhodotorula mucilaginosa Species 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241001479507 Senecio odorus Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 101710106714 Shutoff protein Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000222068 Sporobolomyces <Sporidiobolaceae> Species 0.000 description 1
- 241000123675 Sporobolomyces roseus Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000187191 Streptomyces viridochromogenes Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 244000288561 Torulaspora delbrueckii Species 0.000 description 1
- 235000014681 Torulaspora delbrueckii Nutrition 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 241000589636 Xanthomonas campestris Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 101500015412 Zea mays Ubiquitin Proteins 0.000 description 1
- JAWMENYCRQKKJY-UHFFFAOYSA-N [3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-ylmethyl)-1-oxa-2,8-diazaspiro[4.5]dec-2-en-8-yl]-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]methanone Chemical compound N1N=NC=2CN(CCC=21)CC1=NOC2(C1)CCN(CC2)C(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F JAWMENYCRQKKJY-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229960005475 antiinfective agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000005667 attractant Substances 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- OCBHHZMJRVXXQK-UHFFFAOYSA-M benzyl-dimethyl-tetradecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 OCBHHZMJRVXXQK-UHFFFAOYSA-M 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000004649 carbonic acid derivatives Chemical class 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 229960004830 cetylpyridinium Drugs 0.000 description 1
- NEUSVAOJNUQRTM-UHFFFAOYSA-N cetylpyridinium Chemical compound CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 NEUSVAOJNUQRTM-UHFFFAOYSA-N 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000031902 chemoattractant activity Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229940000425 combination drug Drugs 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 101150044717 cyt1Aa gene Proteins 0.000 description 1
- 101150034285 cytB gene Proteins 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000005869 estivation Effects 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 230000002140 halogenating effect Effects 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006266 hibernation Effects 0.000 description 1
- 230000002962 histologic effect Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 231100001225 mammalian toxicity Toxicity 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 230000001343 mnemonic effect Effects 0.000 description 1
- 230000037230 mobility Effects 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000037434 nonsense mutation Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 101150017854 petB gene Proteins 0.000 description 1
- 101150040669 petJ gene Proteins 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229910052615 phyllosilicate Inorganic materials 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 101150098238 pont gene Proteins 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000012514 protein characterization Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000020354 squash Nutrition 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241000700570 unidentified entomopoxvirus Species 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- CPYIZQLXMGRKSW-UHFFFAOYSA-N zinc;iron(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[O-2].[Fe+3].[Fe+3].[Zn+2] CPYIZQLXMGRKSW-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/075—Bacillus thuringiensis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/378,088 US6372480B1 (en) | 1996-04-19 | 1999-08-20 | Pesticidal proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200103145B true ZA200103145B (en) | 2002-07-04 |
Family
ID=23491663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200103145A ZA200103145B (en) | 1999-08-20 | 2001-04-18 | Pesticidal proteins. |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US6372480B1 (es) |
| EP (2) | EP1187922A2 (es) |
| JP (1) | JP2003509018A (es) |
| KR (1) | KR20010099680A (es) |
| CN (1) | CN1408023A (es) |
| AR (1) | AR029644A1 (es) |
| AU (1) | AU784060B2 (es) |
| BR (1) | BR0007027A (es) |
| CA (1) | CA2344469A1 (es) |
| HU (1) | HUP0103794A2 (es) |
| ID (1) | ID29577A (es) |
| IL (2) | IL142170A0 (es) |
| RO (1) | RO123431B1 (es) |
| RU (1) | RU2267535C2 (es) |
| UA (1) | UA84666C2 (es) |
| WO (1) | WO2001014417A2 (es) |
| ZA (1) | ZA200103145B (es) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372480B1 (en) | 1996-04-19 | 2002-04-16 | Mycogen Corporation | Pesticidal proteins |
| CA2371442A1 (en) * | 1999-05-04 | 2000-11-09 | Monsanto Technology Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
| AU2001290919A1 (en) | 2000-09-12 | 2002-03-26 | Monsanto Technology Llc | Insect inhibitory bacillus thuringiensis proteins, fusions, and methods of use therefor |
| JP2010207225A (ja) * | 2002-02-13 | 2010-09-24 | Dow Global Technologies Inc | シュードモナス菌及び近縁細菌における極限酵素遺伝子の過剰表現 |
| AU2003218114B2 (en) * | 2002-03-11 | 2008-09-18 | Athenix Corporation | Integrated system for high throughput capture of genetic diversity |
| US7309785B1 (en) * | 2003-10-03 | 2007-12-18 | Dow Agrosciences Llc | Modified chimeric Cry35 proteins |
| US7524810B1 (en) * | 2003-10-03 | 2009-04-28 | Dow Agrosciences Llc | Modified Cry34 proteins |
| US7985892B1 (en) | 2004-06-29 | 2011-07-26 | Dow Agrosciences Llc | Truncated Cry35 proteins |
| US7323556B2 (en) * | 2004-09-29 | 2008-01-29 | Pioneer Hi-Bred International, Inc. | Corn event DAS-59122-7 and methods for detection thereof |
| TWM318228U (en) * | 2006-11-02 | 2007-09-01 | Datafab Sys Inc | Structure for protecting terminal of memory card adapter |
| US20110136731A1 (en) * | 2007-12-11 | 2011-06-09 | Bauer Leonora S | Protein having pesticidal activity, dna encoding the protein, and noxious organism-controlling agent that controls the insect pest, emerald ash borer (eab), agrilus planipennis |
| US8216997B2 (en) | 2008-08-14 | 2012-07-10 | Acceleron Pharma, Inc. | Methods for increasing red blood cell levels and treating anemia using a combination of GDF traps and erythropoietin receptor activators |
| LT3494986T (lt) | 2008-08-14 | 2020-07-10 | Acceleron Pharma Inc. | Gdf gaudyklės |
| CA2747826A1 (en) * | 2008-12-23 | 2010-07-01 | Athenix Corporation | Axmi-150 delta-endotoxin gene and methods for its use |
| CA2754845A1 (en) * | 2009-03-11 | 2010-12-09 | Athenix Corporation | Axmi-030 insecticidal protein from bacillus thuringiensis and methods for use |
| UA116612C2 (uk) | 2010-04-23 | 2018-04-25 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | ТРАНСГЕННА РОСЛИНА КУКУРУДЗИ, ЯКА ПРОДУКУЄ БІЛКИ Cry34Ab1, Cry35Ab1 І Cry6Aa1 ДЛЯ ЗАПОБІГАННЯ РОЗВИТКУ СТІЙКОСТІ У КУКУРУДЗЯНОГО КОРЕНЕВОГО ЖУКА (Diabrotica spp.) |
| CN108026149B (zh) * | 2015-08-17 | 2022-04-29 | 美国陶氏益农公司 | 工程化的cry6a杀虫蛋白 |
| US10889830B2 (en) | 2016-09-30 | 2021-01-12 | Dow Agrosciences Llc | Binary insecticidal Cry toxins |
| CA3048497A1 (en) | 2017-02-24 | 2018-08-30 | Flagship Pioneering Innovations V, Inc. | Compositions and related methods for modulating endosymbionts |
| EP3781692A1 (en) * | 2018-04-20 | 2021-02-24 | Agbiome, Inc. | Pesticidal proteins and methods of use |
| WO2019211850A1 (en) | 2018-05-03 | 2019-11-07 | Evogene Ltd. | Insecticidal polypeptides and use thereof |
| CN113615456B (zh) * | 2021-08-11 | 2022-10-21 | 华南师范大学 | 一种防治夜蛾科农业害虫的方法及杀虫剂 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448885A (en) | 1981-04-27 | 1984-05-15 | Board Of The Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
| US4467036A (en) | 1981-11-12 | 1984-08-21 | The Board Of Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4695462A (en) | 1985-06-28 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of biological pesticides |
| US4695455A (en) | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
| US4797276A (en) | 1985-03-22 | 1989-01-10 | Mycogen Corporation | Control of cotton boll weevil, alfalfa weevil, and corn rootworm via contact with a strain of Bacillus thuringiensis |
| US4918006A (en) | 1985-07-01 | 1990-04-17 | E. I. Du Pont De Nemours And Company | Gene coding for insecticidal crystal protein |
| US4853331A (en) | 1985-08-16 | 1989-08-01 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera |
| US4948734A (en) | 1987-08-12 | 1990-08-14 | Mycogen Corporation | Novel isolates of bacillus thuringiensis having activity against nematodes |
| US5151363A (en) | 1990-07-27 | 1992-09-29 | Mycogen Corporation | Isolates of Bacillus thuringiensis that are active against nematodes |
| US4849217A (en) | 1987-11-19 | 1989-07-18 | Mycogen Corporation | Novel isolates of bacilus thuringiensis having activity against the alfalfa weevil, hypera brunneipennis |
| NZ230375A (en) | 1988-09-09 | 1991-07-26 | Lubrizol Genetics Inc | Synthetic gene encoding b. thuringiensis insecticidal protein |
| US5135867A (en) | 1988-11-01 | 1992-08-04 | Mycogen Corporation | Gene encoding a lepidopteran-active toxin from Bacillus thuringiensis isolate denoted B.t. .PS81GG active against lepidopteran pests |
| GB9009571D0 (en) | 1990-04-27 | 1990-06-20 | Univ Singapore | Expression of insecticidal proteins |
| CA2042868A1 (en) | 1990-06-11 | 1991-12-12 | Kenneth E. Narva | Bacillus thuringiensis microbes active against nematodes, and genes encoding novel nematode-active toxins cloned from bacillus thuringiensis isolates |
| US5632987A (en) | 1992-12-31 | 1997-05-27 | Mycogen Corporation | Bacillus thuringiensis toxins active against corn rootworm larvae |
| US5208077A (en) | 1990-11-09 | 1993-05-04 | Florida Wire And Cable Company | Method for a composite material comprising coated and filled metal strand for use in prestressed concrete, stay cables for cable-stayed bridges and other uses |
| US5204100A (en) | 1991-02-01 | 1993-04-20 | Ciba-Geigy Corporation | Baciullus thuringiensis strains active against coleopteran insects |
| US5723758A (en) | 1991-09-13 | 1998-03-03 | Mycogen Corporation | Bacillus thuringiensis genes encoding lepidopteran-active toxins |
| ATE244259T1 (de) | 1992-02-12 | 2003-07-15 | Chromagen Inc | Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen |
| AU5987794A (en) | 1992-12-31 | 1994-08-15 | Mycogen Corporation | Novel (bacillus thuringiensis) toxins active against corn rootworm larvae |
| WO1995002694A2 (en) | 1993-07-15 | 1995-01-26 | Mycogen Corporation | Materials and methods for the control of calliphoridae pests |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6083499A (en) | 1996-04-19 | 2000-07-04 | Mycogen Corporation | Pesticidal toxins |
| US6372480B1 (en) | 1996-04-19 | 2002-04-16 | Mycogen Corporation | Pesticidal proteins |
| GB9618083D0 (en) * | 1996-08-29 | 1996-10-09 | Mini Agriculture & Fisheries | Pesticidal agents |
| CN1324407A (zh) | 1998-10-23 | 2001-11-28 | 麦考根公司 | 编码大约15kDa和大约45kDa的杀虫蛋白质的植物最优化的多核苷酸 |
| CA2371442A1 (en) | 1999-05-04 | 2000-11-09 | Monsanto Technology Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
-
1999
- 1999-08-20 US US09/378,088 patent/US6372480B1/en not_active Expired - Lifetime
-
2000
- 2000-08-21 BR BR0007027-0A patent/BR0007027A/pt not_active Application Discontinuation
- 2000-08-21 ID IDW00200101099A patent/ID29577A/id unknown
- 2000-08-21 EP EP00957635A patent/EP1187922A2/en not_active Withdrawn
- 2000-08-21 KR KR1020017004466A patent/KR20010099680A/ko not_active Application Discontinuation
- 2000-08-21 CA CA002344469A patent/CA2344469A1/en not_active Abandoned
- 2000-08-21 AU AU69225/00A patent/AU784060B2/en not_active Expired
- 2000-08-21 JP JP2001518746A patent/JP2003509018A/ja active Pending
- 2000-08-21 RU RU2001111046/13A patent/RU2267535C2/ru not_active IP Right Cessation
- 2000-08-21 RO ROA200100438A patent/RO123431B1/ro unknown
- 2000-08-21 HU HU0103794A patent/HUP0103794A2/hu not_active Application Discontinuation
- 2000-08-21 WO PCT/US2000/022942 patent/WO2001014417A2/en active Application Filing
- 2000-08-21 CN CN00801757A patent/CN1408023A/zh active Pending
- 2000-08-21 EP EP07123282A patent/EP2036982A1/en not_active Withdrawn
- 2000-08-21 UA UA2001042640A patent/UA84666C2/ru unknown
- 2000-08-21 IL IL14217000A patent/IL142170A0/xx active IP Right Grant
- 2000-08-22 AR ARP000104334A patent/AR029644A1/es not_active Application Discontinuation
-
2001
- 2001-03-21 IL IL142170A patent/IL142170A/en not_active IP Right Cessation
- 2001-04-18 ZA ZA200103145A patent/ZA200103145B/en unknown
-
2002
- 2002-03-15 US US10/099,278 patent/US6900371B2/en not_active Expired - Fee Related
-
2005
- 2005-01-18 US US11/037,780 patent/US7355003B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP2036982A1 (en) | 2009-03-18 |
| AU6922500A (en) | 2001-03-19 |
| JP2003509018A (ja) | 2003-03-11 |
| CA2344469A1 (en) | 2001-03-01 |
| RU2267535C2 (ru) | 2006-01-10 |
| AU784060B2 (en) | 2006-01-19 |
| UA84666C2 (ru) | 2008-11-25 |
| AR029644A1 (es) | 2003-07-10 |
| US6372480B1 (en) | 2002-04-16 |
| BR0007027A (pt) | 2001-07-10 |
| CN1408023A (zh) | 2003-04-02 |
| IL142170A0 (en) | 2002-03-10 |
| WO2001014417A3 (en) | 2002-01-24 |
| RO123431B1 (ro) | 2012-04-30 |
| US7355003B2 (en) | 2008-04-08 |
| ID29577A (id) | 2001-09-06 |
| HUP0103794A2 (en) | 2002-07-29 |
| US20030106093A1 (en) | 2003-06-05 |
| WO2001014417A2 (en) | 2001-03-01 |
| US6900371B2 (en) | 2005-05-31 |
| IL142170A (en) | 2008-11-26 |
| KR20010099680A (ko) | 2001-11-09 |
| US20050155112A1 (en) | 2005-07-14 |
| EP1187922A2 (en) | 2002-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0914439B1 (en) | Pesticidal toxins | |
| US7355003B2 (en) | Pesticidal proteins | |
| EP0758385B1 (en) | Chimeric delta-endotoxin expression in pseudomonas fluorescens | |
| AU699752B2 (en) | Improved (bacillus thuringiensis) delta-endotoxin | |
| US5632987A (en) | Bacillus thuringiensis toxins active against corn rootworm larvae | |
| US5723758A (en) | Bacillus thuringiensis genes encoding lepidopteran-active toxins | |
| US6274721B1 (en) | Toxins active against pests | |
| US5686069A (en) | Protein toxins active against lepidopteran pests | |
| AU3608693A (en) | Process for controlling scarab pests with (bacillus thuringiensis) isolates | |
| EP0612218B1 (en) | NOVEL COLEOPTERAN-ACTIVE $i(BACILLUS THURINGIENSIS) ISOLATES AND GENES ENCODING COLEOPTERAN-ACTIVE TOXINS | |
| AU2651992A (en) | Process for controlling lepidopteran pests | |
| US20110003736A1 (en) | Pesticidal proteins | |
| WO1999033991A2 (en) | TOXINS ACTIVE AGAINST $i(OSTRINIA NUBILALIS) | |
| WO1994016079A2 (en) | Novel bacillus thuringiensis toxins active against corn rootworm larvae | |
| AU2003203829B2 (en) | Pesticidal Toxins | |
| AU6704098A (en) | Pesticidal bacillus thuringiensis strains | |
| US6051550A (en) | Materials and methods for controlling homopteran pests |