WO2024096259A1 - Composition cosmétique contenant du bakuchiol pour le soin de la peau - Google Patents

Composition cosmétique contenant du bakuchiol pour le soin de la peau Download PDF

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WO2024096259A1
WO2024096259A1 PCT/KR2023/010903 KR2023010903W WO2024096259A1 WO 2024096259 A1 WO2024096259 A1 WO 2024096259A1 KR 2023010903 W KR2023010903 W KR 2023010903W WO 2024096259 A1 WO2024096259 A1 WO 2024096259A1
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fruit extract
bakuchiol
skin
improvement
present
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PCT/KR2023/010903
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English (en)
Korean (ko)
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이혜진
예상현
전승현
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주식회사 엘지생활건강
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Publication of WO2024096259A1 publication Critical patent/WO2024096259A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a cosmetic composition and a quasi-drug composition comprising bakuchiol, white thistle fruit extract, and Gaja fruit extract; How to improve your skin; and its uses.
  • UV-A ultraviolet rays
  • UV-B ultraviolet rays
  • UV-B air pollution
  • UV-A ultraviolet rays
  • UV-B ultraviolet rays
  • UV-B air pollution
  • UV-B has a higher energy content than UV-A, so it is considered the main cause of breakdown of skin homeostasis.
  • UV-B promotes the expression of Matrix metalloproteinase-1 (MMP-1), which is a major collagen decomposition factor that breaks down collagen type I, causing skin to lose tension and contributing to the formation of wrinkles.
  • MMP-1 Matrix metalloproteinase-1
  • Bax Bcl-2-related protein-x
  • Cosmetic ingredients are generally known to cause various skin problems such as inflammation, rashes, and edema (Maibach. H. I., Contact Dermatitis, 6. 369-404, 1980).
  • various substances used as raw materials for functional cosmetics also have problems of low stability or insignificant effect on the skin.
  • adenosine, protein derived from animal placenta, and chlorella extract are known as cosmetics for improving skin wrinkles and elasticity.
  • the most widely known extracts, such as chlorella extract, have minimal effects, so it is difficult to expect practical effects on improving skin elasticity and improving wrinkles.
  • One object of the present invention is to provide a cosmetic composition comprising bakuchiol, white thistle fruit extract, and soybean fruit extract.
  • Another object of the present invention is to provide a quasi-drug composition containing bakuchiol, white thistle fruit extract, and Gaja fruit extract.
  • Another object of the present invention is to provide a method for improving skin, comprising the step of applying or administering a composition containing bakuchiol, white thistle fruit extract, and Gaja fruit extract to the skin of a subject.
  • Another object of the present invention is to provide a skin improving use of a composition containing bakuchiol, white thistle fruit extract, and soybean fruit extract.
  • the bakuchiol, white thistle fruit extract, and Gaja fruit extract of the present invention have excellent skin improvement effects, they are highly utilized as cosmetic compositions and quasi-drug compositions for skin improvement. Accordingly, various industrial applications such as cosmetics and pharmaceutical products can be expected.
  • Figure 1 is a graph showing the RARG gene activity of white thistle fruit extract.
  • Figure 2 is a graph showing the RAR activity of bakuchiol and white thistle fruit extract.
  • Figure 3 is a result graph showing the results of evaluation of human application of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil.
  • Figure 4 is a photo showing the results of evaluation of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil on the human body.
  • Figure 5 is a 3D skin cross-sectional result showing the photoaging improvement effect of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil.
  • Figure 6 is a result graph showing the photoaging improvement effect of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil.
  • Figure 7 is a result graph showing the skin irritation and skin inflammation improvement effects of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil.
  • One aspect of the present invention provides a cosmetic composition comprising bakuchiol, white thistle fruit extract, and soybean fruit extract.
  • Bochiol in the present invention is a natural ingredient derived from plants and is a meroterpene of the terpenophenol series. It is a material extracted from Bogolji seeds and is known to have antioxidant activity. Bakuchiol began to be referred to as plant-based natural retinol because it has a similar gene expression pattern to retinoids in the skin, but the two substances have no structural similarity and there are differences in their mechanisms of action. Bakuchiol has low expression and activity of retinoic acid receptors induced by retinol, and is known to have lower wrinkle improvement efficacy than retinol.
  • Bakuchiol of the present invention may have the following structure.
  • the method of obtaining bakuchiol is not particularly limited, and materials collected from nature, chemically synthesized by methods known in the art, or commercially available can be used.
  • the bakuchiol may include all derivatives of bakuchiol within the range expected by those skilled in the art, and are included without limitation in the present invention as long as they have the same effect.
  • Terminalia medicinal terminalia fruit
  • gaja or villus gaja belonging to the Sagunaceae family. It is being used.
  • Oriental medicine it has a sharp taste and mild medicinal properties and is widely used to treat astringents, hemostasis, and chronic laryngitis.
  • compositions of the present invention may include extracts of the fruit of thistle, Gaja.
  • extract in the present invention refers to the resulting liquid component obtained by immersing the target material in various solvents and then extracting it at room temperature or at a heated state for a certain period of time, and the solid content obtained by removing the solvent from the liquid component. it means.
  • the extraction may be performed one or more times, but is not limited thereto.
  • it can be comprehensively interpreted to include all of the filtrate, dilution, concentrate, crude, purified, dried, etc. of the resultant.
  • the white thistle fruit extract and the white thistle fruit extract and the white thistle fruit extract provided by the present invention include the extract itself, such as an extract obtained by extracting the white thistle fruit or the white thistle fruit, a purified product obtained by enzymatically decomposing the extract, or a mixture thereof, and the like. It can be interpreted to include extracts of all formulations that can be formed using extracts, and/or dried products thereof.
  • the method of extracting the white thistle fruit extract and the Gaja fruit extract of the present invention is not particularly limited, and can be extracted according to a method commonly used in the art.
  • Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, and reflux extraction, which may be performed alone or by combining two or more methods.
  • Solvents used in the preparation of the extract include water, alcohol, or a mixed solvent thereof, and these may be used alone or in a mixture of two or more types, and when alcohol is used as a solvent, specifically, carbon number 1 Alcohols from 4 to 4, ethanol, methanol or butanol can be used.
  • the bakuchiol, white thistle fruit extract, and Gaja fruit extract are used in a weight ratio of 1:0.1:0.1 to 1:100:100, specifically 1:1:1 to 1:50:50, more specifically 1: It may be mixed at a weight ratio of 1:1 to 1:10:10.
  • Retinoic Acid Receptor refers to a receptor present in the nucleus required for the action of retinoic acid, which can be considered the active form of vitamin A.
  • RAR Retinoic Acid Receptor
  • retinoic acid When retinoic acid is applied to the skin, the number of fibroblasts that synthesize collagen increases and the synthesis of new collagen increases. New collagen created from fibroblasts gathers at the area where the epidermis and dermis meet, ultimately regenerating the damaged dermis, improving wrinkles, and improving skin elasticity.
  • Retinoic acid is known to have the effect of regenerating collagen fibers as well as manganese elastic fibers.
  • Retinoic Acid Receptor Gamma (RARG, RAR ⁇ ) is a retinoid receptor gene and refers to a gene that requires binding and increased activity of retinoic acid for reactions such as wrinkle improvement by retinol to occur.
  • “ITGA6 (Integrin alpha 6)” is known to play a role in strengthening the skin barrier, and laminin maintains the skin structure by connecting and fixing the dermis and epidermis in the basement membrane between the dermis and epidermis to provide stability. It is a gene that plays an anti-aging role by directly combining with .
  • skin barrier strengthening may mean any action that improves the function of the skin barrier, which is located on the outermost layer of the skin and prevents moisture and nutrition loss.
  • RARG gene expression increased when treated with white thistle fruit extract ( Figure 1), and when bakuchiol and white thistle fruit extract were treated together, compared to treatment with bakuchiol alone. It was confirmed that RAR activity increased ( Figure 2).
  • the cosmetic composition may further include mastic gum or rosehip oil, but is not limited thereto.
  • mastic gum is made from dried mastic tree sap and has long been known to have been used as medicine for stomach health in Greece.
  • rosehip oil in the present invention refers to oil extracted from the fruits or seeds of wild rose (Rosa canina). Rosehip oil is known to improve moisturizing power by blocking moisture loss.
  • the mastic gum is used in an amount of 0.0001 to 100, specifically 0.001 to 50, more specifically 0.01 to 10% by weight, based on 1% by weight of bakuchiol
  • the rosehip oil is used in an amount of 0.001 to 100, specifically, based on 1% by weight of bakuchiol. It may be mixed at 0.01 to 10% by weight, more specifically 0.1 to 5% by weight.
  • the composition may have a skin improvement effect.
  • skin improvement in the present invention may be one or more selected from the group consisting of wrinkle improvement, elasticity improvement, aging improvement, skin irritation improvement, and inflammation improvement, but is not limited thereto.
  • the term "improvement” refers to any action that at least reduces the severity of symptoms, such as improving parameters related to the alleviation or treatment of a condition, including improving wrinkles, improving elasticity, improving aging, improving skin irritation, and improving inflammation. Any one or more may be included.
  • the skin improvement may be achieved through activating the Retinoic Acid Receptor, increasing Retinoic Acid Receptor Gamma gene expression, increasing ITGA6 gene expression, promoting collagen synthesis, or reducing the expression of inflammatory substances. It is not limited to this.
  • the present inventors used bakuchiol, white thistle fruit extract, and soybean thistle fruit extract; It was confirmed that the composition further comprising mastic gum and/or rosehip oil in the mixture had an excellent skin improvement effect. More specifically, bakuchiol, white thistle fruit extract and soybean fruit extract; The composition further comprising mastic gum and/or rosehip oil in the mixture activates Retinoic Acid Receptor, increases Retinoic Acid Receptor Gamma gene expression, increases ITGA6 gene expression, and collagen synthesis. It was confirmed that the effect of promoting or reducing the expression of inflammatory substances was excellent.
  • composition further comprising mastic gum and/or rosehip oil in the mixture had excellent skin improvement effects such as wrinkle improvement, elasticity improvement, aging improvement, skin irritation improvement, and inflammation improvement.
  • wrinkles of the present invention can be broadly divided into facial wrinkles caused by facial muscles used to make facial expressions and fine wrinkles caused by general or local deterioration of the skin. Specifically, wrinkles can be caused by genes, a decrease in collagen and elastic fibers present in the skin dermis, and external environments. For the purposes of the present invention, wrinkles may be used to include both facial expression wrinkles and fine wrinkles, but are not limited thereto.
  • elasticity refers to the force that holds the skin taut when pressed with a finger
  • elasticity improvement or “elasticity enhancement” refers to increasing the elasticity of the skin by strengthening the subcutaneous fat layer structure of the subject. This may mean increasing skin elasticity using the composition of the invention.
  • Collagen is a fibrous protein found in most animals, especially mammals, it makes up most of all connective tissues in the body, such as skin and cartilage, and fibroblasts are the most common cells in the body. (fibroblasts) produce and secrete collagen.
  • Gelatin which is widely used in cooking, food, and the pharmaceutical industry, is made by irreversibly hydrolyzing collagen. A decrease in collagen is the main cause of wrinkles and reduced skin elasticity, so collagen synthesis is essential for improving wrinkles and improving elasticity.
  • the amount of collagen synthesis significantly increased when treated with bakuchiol, white thistle fruit extract, and Gaja fruit extract compared to treatment with bakuchiol alone.
  • the amount of collagen synthesis was further increased when mastic gum and/or rosehip oil were used in addition to bakuchiol, white thistle fruit extract, and Gaja fruit extract (Table 3).
  • aging refers to the phenomenon of decline in biological structure and function over time, and the anti-aging of the present invention is not limited to the extent to which aging is improved, such as by preventing, suppressing, or delaying aging. No. In the present invention, wrinkle improvement and anti-aging may be achieved by promoting collagen synthesis, but are not limited thereto.
  • UV the biggest cause of extrinsic aging, is caused by UV, and UV can be divided into UVA, UVB, and UVC depending on the wavelength.
  • UVB ultraviolet-B
  • UVA ultraviolet-A
  • Wrinkle formation a representative phenomenon of skin aging, is mainly caused by a decrease in collagen present in the dermal tissue.
  • Dermal fibroblasts that make up the dermal layer regulate the expression of collagen, elastin, and MMP, and UV irradiation reduces structural proteins such as collagen and elastin. Reduces the synthesis of and increases the expression of MMPs
  • photoaging refers to a phenomenon in which skin damage occurs, such as a decrease in skin elasticity and moisture and the formation of wrinkles, upon repeated or long-term exposure to ultraviolet rays of the sun.
  • the skin barrier strengthening effect is excellent, and the cell protection effect against UV rays to prevent collagen decomposition is excellent. It was confirmed that the photoaging improvement effect significantly increased ( Figures 5 and 6)
  • skin irritation improvement in the present invention means alleviating and soothing erythema, itchiness, or irritated skin areas, etc., and may be a concept that includes alleviating skin irritation. Examples include, but are not limited to, alleviating skin itching, alleviating skin pain, reducing moisture loss from the skin, and/or reducing redness.
  • inflammation is a kind of in vivo response to damage or infection of a specific tissue, the main mediator is immune cells.
  • the purpose of this inflammation is to minimize tissue damage, eliminate infectious agents, and regenerate tissues.
  • improvement in skin irritation, soothing and regeneration may be due to inhibition of inflammatory substances (IL-1a), but is not limited thereto.
  • Retinol or bakuchiol is known as a substance that induces stimulation of inflammatory factors, and has problems such as redness, irritation, and inflammation (British Journal of Dermatology (2019) 180, pp289-296).
  • cosmetic composition refers to solution, external ointment, cream, foam, nutritional lotion, softening lotion, perfume, pack, softening water, emulsion, makeup base, essence, soap, liquid cleanser, bath additive, sunscreen cream, It may be a formulation selected from the group consisting of sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. , but is not limited to this.
  • the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers that are blended with general skin cosmetics, and common ingredients include, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, Chelating agents, pigments, preservatives, fragrances, etc. may be appropriately mixed, but are not limited thereto.
  • Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation of the cosmetic composition.
  • the carrier ingredients include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, and oxidation.
  • Zinc, etc. may be used, but are not limited thereto. These may be used alone or in combination of two or more types.
  • a solvent, solubilizing agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, etc. can be used, especially cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan. may be used, but is not limited thereto. These may be used singly or two or more types may be used.
  • the carrier components include water, liquid diluents such as ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and miso.
  • Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more types.
  • alkali metal salts of fatty acids hemiester salts of fatty acids, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, fatty alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier ingredients. However, it is not limited to this. These may be used individually or in combination of two or more types.
  • lactose When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silcate, polyamide powder, or mixtures thereof may be used as the carrier ingredient, and especially in the case of a spray, chloro May contain propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.
  • propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.
  • composition of the present invention may be included in the composition of the present invention within a range that does not exceed the maximum usage level specified in the Regulations on Cosmetic Safety Standards, etc. and the Chinese 'Cosmetic Safety and Technology Code'.
  • Another aspect of the present invention provides a quasi-drug composition comprising bakuchiol, white thistle fruit extract, and Gaja fruit extract.
  • the composition may have a skin improvement effect.
  • the “skin improvement” is as described above.
  • the quasi-drug composition may additionally include mastic gum or rosehip oil, but is not limited thereto.
  • quasi-drug in the present invention refers to products that have a milder effect than pharmaceuticals among products used for the purpose of diagnosing, treating, improving, alleviating, treating, or preventing diseases in humans or animals.
  • quasi-drugs under the Pharmaceutical Affairs Act exclude products used for pharmaceutical purposes and include products used to treat or prevent diseases in humans and animals, and products that have a mild or no direct effect on the human body.
  • the quasi-drugs may include external skin preparations and personal hygiene products. More specifically, it may be a disinfectant cleaner, shower foam, garnish, wet tissue, detergent soap, hand wash, or ointment, but is not limited thereto.
  • the composition when using the composition according to the present invention as a quasi-drug additive, the composition can be added as is or used together with other quasi-drugs or quasi-drug components, and can be used appropriately according to conventional methods.
  • the mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.
  • Another aspect of the present invention provides a method for improving skin, comprising the step of applying or administering a composition containing bakuchiol, white thistle fruit extract, and Gaja fruit extract to the skin of a subject.
  • composition may further include, but is not limited to, mastic gum or rosehip oil.
  • the composition may be a cosmetic or quasi-drug composition, but is not limited thereto.
  • subject of the present invention refers to any animal, including humans, that needs improvement of skin wrinkles, improvement of elasticity, improvement of aging, improvement of irritation, or improvement of inflammation, lacks collagen, or has erythematous, itchy or irritated skin, and said animal It may be, but is not limited to, not only humans but also mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that require treatment for similar symptoms.
  • the term "application” refers to any method of contacting the composition according to the present invention to the skin of an individual by any appropriate method, and the purpose is to allow the composition to be absorbed into the skin.
  • composition of the present invention When the composition of the present invention is applied to the skin of an individual, it may have the effect of improving wrinkles, improving elasticity, improving aging, improving skin irritation, and improving inflammation, and the composition may be applied or administered to the individual in an amount effective to produce the above effects. It may include steps.
  • the term “administration” is a general term for providing a predetermined substance to an individual by any appropriate method, and the administration route can be administered through any general route as long as it can reach the target skin. there is.
  • Another aspect of the present invention provides a skin improving use of a composition comprising bakuchiol, white thistle fruit extract, and soybean thistle fruit extract.
  • composition may further include, but is not limited to, mastic gum or rosehip oil.
  • the composition may be a cosmetic or quasi-drug composition, but is not limited thereto.
  • the bakuchiol used in the present invention is Sytenol A (raw material name) from Sytheon, the white thistle fruit extract is Silymarin phytosome (raw material name) from Indena, the crab fruit extract is Synastol TC (raw material name) from Sytheon, and the rosehip oil is ROSEHIP OIL REFINED from TEXTRON TECNICA. (name of raw material), Mastic Black: Purchased and used from Novoretin (name of raw material) from Mibelle.
  • Example 1-1 Evaluation of RARG gene activity in white thistle fruit extract
  • Silybum Marianum Fruit Extract was used as a material containing 17.9% of silymarin as an indicator ingredient.
  • RARG retinoic acid receptor gene in the cell nucleus
  • human dermal firbroblasts were distributed in 6-well plates at 5*10 5 cells/well and cultured in a CO-2 incubator overnight. Substances were added to the culture medium at different concentrations and cultured for 24 hours.
  • RNA was extracted according to the protocol using the RNA-spin Total RNA Extraction Kit (Intron Biotechnology, Korea), and real-time qPCR was performed using a Taqman probe to compare and analyze the RARG gene expression level in the white thistle fruit extract. After doing so, the measurement results were confirmed (Figure 1).
  • Example 1-2 Evaluation of RAR activity of bakuchiol and white thistle fruit extracts
  • Bakuchiol (BK) and white thistle fruit extract (SM) were mixed in DMEM media (Dulbecco Modified Eagle Medium) at the concentrations shown in Table 1 below and then treated with cells. Specifically, to measure the degree of RAR activity caused by retinoic acid converted from retinol entering the cell nucleus and binding to the retinoic acid receptor (RAR), RAR ⁇ Reporter (Luc)-HEK293 cell line (BPS bioscience, USA) was purchased. An experiment was performed. Cells were distributed in 96 well plates at 10 4 cells/well and cultured in a CO 2 incubator overnight. Bakuchiol (BK) and white thistle fruit extract (SM) were added to the culture medium at various concentrations and cultured for 24 hours.
  • DMEM media Dulbecco Modified Eagle Medium
  • Example 2 Evaluation of ITGA6 gene expression level of bakuchiol, gizzard fruit extract, mastic gum, and rosehip oil
  • Bakuchiol, hazelnut fruit extract, mastic gum, and rosehip oil were mixed in DMEM media at the concentrations shown in Table 2 below and then treated with cells.
  • keratinocytes HaCaT were distributed in 6-well plates at 5*10 5 cells/well and cultured in a CO ⁇ 2 incubator overnight. Substances were added to the culture medium at different concentrations and cultured for 24 hours.
  • RNA was extracted according to the protocol using the RNA-spin Total RNA Extraction Kit (Intron Biotechnology, Korea), and real-time qPCR was performed using a Taqman probe to compare and analyze the ITGA6 gene expression level and confirm the measurement results. (Table 2).
  • Example 3 Confirmation of the wrinkle or elasticity improvement effect of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil
  • Example 3-1 Evaluation of collagen synthesis enhancement of bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum, and rosehip oil.
  • Bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil were mixed in DMEM media at the concentrations shown in Table 3 below and then treated with cells.
  • human skin fibroblasts (Fibroblast) were first dispensed at 2*10 4 cells/well in a 24-well plate and cultured in a CO 2 incubator overnight. After removing the culture medium from each well, lightly washing with PBS and replacing each well with the culture medium again, substances were added at the concentrations and combinations described and cultured for 24 hours. Next, the culture medium was obtained, and the procollagen secreted into the culture medium was measured using a procollagen type C-peptide EIA kit (MK-101, Takara Bio, Japan).
  • bakuchiol, white thistle fruit extract and Gaja fruit extract of the present invention bakuchiol, white thistle fruit extract, gizzard fruit extract and mastic gum
  • bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum, and rosehip oil showed excellent effects in improving skin wrinkles and improving elasticity.
  • Example 3-2 Evaluation of bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum and rosehip oil for human application
  • a cream formulation containing 0.1% bakuchiol was prepared and applied to a total of 12 underprivileged women in their 40s and 50s twice a day (once in the morning and once in the evening) under the eyes, nasolabial folds, and cheeks for a total of 4 weeks. did.
  • the wrinkle improvement rate was measured using Antera 3D for under-eye and nasolabial folds and Cutometer for cheek elasticity, and the average wrinkle improvement rate for all subjects and the improvement image of one representative person were shown ( Figures 3 and 4).
  • the improvement rate of wrinkles under the eyes increased by about 5.3 times, and when treated with bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil, the depth and length of nasolabial folds decreased by about 3.1 times. .
  • Example 4 Confirmation of the effects of bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil on improving photoaging, skin irritation, and skin inflammation
  • Example 4-1 Confirmed the photoaging improvement effect of bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum, and rosehip oil.
  • Tegoscience Neoderm®-ED a 3D reconstructed human skin model consisting of the epidermis and dermis, was injected with bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum, and rosehip oil (Booster 4). bell);
  • Booster 4 rosehip oil
  • the cells were treated with bakuchiol and retinol over night, and then cultured under UV irradiation conditions with 50 mJ/cm2 UVB twice a day for a total of 3 days.
  • paraffin blocks were prepared and stained with Masson's trichrome to confirm the 3D skin cross-section (Figure 5, red: epidermis, blue: dermal collagen). Afterwards, the dermal collagen area was measured using the publicly available software Image J ( Figure 6).
  • the epidermal barrier protection effect is excellent, and the dermal collagen area recovery ability is significantly improved compared to treatment with retinol or bakuchiol alone. Confirmed.
  • the bakuchiol, white thistle fruit extract, Gaja fruit extract, mastic gum, and rosehip oil of the present invention are excellent in not only the wrinkle improvement and elasticity improvement effects, but also the photoaging improvement effects.
  • Example 4-2. Confirmed the effectiveness of bakuchiol, white thistle fruit extract, gizzard fruit extract, mastic gum, and rosehip oil to improve skin irritation and skin inflammation.
  • Example 4-1 After culturing the 3D human skin model with extract treatment and UVB treatment in the same manner as in Example 4-1, the culture medium was obtained and secreted inflammatory substances ( The amount of IL-1a) was measured (FIG. 7).

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  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
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  • Gerontology & Geriatric Medicine (AREA)
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Abstract

La présente invention concerne : une composition cosmétique et une composition de produit quasi-médicamenteux, contenant chacune du bakuchiol, un extrait de fruit de chardon Marie, et un extrait de fruit de gajar ; un procédé de soins de la peau ; et une utilisation associée. Avec d'excellents effets de soins de la peau, le bakuchiol, l'extrait de fruit de chardon Marie et l'extrait de fruit de gazar selon la présente invention sont hautement applicables à une composition cosmétique et à une composition de quasi-médicament de soins de la peau. Par conséquent, diverses applications industrielles aux produits cosmétiques, aux produits quasi-médicamenteux, etc. peuvent être attendues.
PCT/KR2023/010903 2022-11-04 2023-07-27 Composition cosmétique contenant du bakuchiol pour le soin de la peau WO2024096259A1 (fr)

Applications Claiming Priority (4)

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KR10-2022-0146332 2022-11-04
KR20220146332 2022-11-04
KR10-2023-0096220 2023-07-24
KR1020230096220A KR20240064514A (ko) 2022-11-04 2023-07-24 바쿠치올을 포함하는, 피부 개선용 화장료 조성물

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WO2024096259A1 true WO2024096259A1 (fr) 2024-05-10

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Citations (3)

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Publication number Priority date Publication date Assignee Title
KR20090077619A (ko) * 2008-01-11 2009-07-15 (주)제이엠비 주름개선에 효과가 있는 한방추출물을 포함하는 화장료조성물
KR102022368B1 (ko) * 2017-12-14 2019-09-18 아이큐어 주식회사 주름 개선 및 리프팅 개선용 화장료 조성물 및 이의 제조방법
KR102286990B1 (ko) * 2019-08-08 2021-08-09 주식회사 코리아나화장품 바쿠치올을 함유하는 나노에멀젼을 유효성분으로 포함하는 화장료 조성물

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090077619A (ko) * 2008-01-11 2009-07-15 (주)제이엠비 주름개선에 효과가 있는 한방추출물을 포함하는 화장료조성물
KR102022368B1 (ko) * 2017-12-14 2019-09-18 아이큐어 주식회사 주름 개선 및 리프팅 개선용 화장료 조성물 및 이의 제조방법
KR102286990B1 (ko) * 2019-08-08 2021-08-09 주식회사 코리아나화장품 바쿠치올을 함유하는 나노에멀젼을 유효성분으로 포함하는 화장료 조성물

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Title
ANONYMOUS: "Bakuchiol: a gentler alternative to retinol", SPA EXECUTIVE, 23 September 2019 (2019-09-23), XP093166518, Retrieved from the Internet <URL:https://spaexecutive.com/2019/09/23/bakuchiol-a-gentler-alternative-to-retinol/> *
JITKA VOSTÁLOVÁ: "Skin Protective Activity of Silymarin and its Flavonolignans", MOLECULES, MDPI AG, CH, vol. 24, no. 6, CH , pages 1022, XP093166521, ISSN: 1420-3049, DOI: 10.3390/molecules24061022 *
WILLIAM R. SWINDELL: "A standardized Terminalia chebula fruit extract alters the expression of genes associated with skin architecture and barrier formation", EUROPEAN JOURNAL OF DERMATOLOGY., JOHN LIBBEY EUROTEXT, FR, vol. 30, no. 5, 1 October 2020 (2020-10-01), FR , pages 469 - 492, XP093166523, ISSN: 1167-1122, DOI: 10.1684/ejd.2020.3882 *

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