WO2024059235A3 - Procédé de recolte de produits à partir de cultures en perfusion - Google Patents

Procédé de recolte de produits à partir de cultures en perfusion Download PDF

Info

Publication number
WO2024059235A3
WO2024059235A3 PCT/US2023/032823 US2023032823W WO2024059235A3 WO 2024059235 A3 WO2024059235 A3 WO 2024059235A3 US 2023032823 W US2023032823 W US 2023032823W WO 2024059235 A3 WO2024059235 A3 WO 2024059235A3
Authority
WO
WIPO (PCT)
Prior art keywords
perfusion cultures
target protein
harvest
protein
disclosure
Prior art date
Application number
PCT/US2023/032823
Other languages
English (en)
Other versions
WO2024059235A2 (fr
Inventor
Xiaoyang Zhao
Oliver KALTENBRUNNER
Gregory T. FRANK
Original Assignee
Amgen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc. filed Critical Amgen Inc.
Publication of WO2024059235A2 publication Critical patent/WO2024059235A2/fr
Publication of WO2024059235A3 publication Critical patent/WO2024059235A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La divulgation concerne des procédés de purification d'une protéine cible, par exemple un anticorps, dérivée d'une cellule hôte telle qu'une cellule de mammifère, à l'aide de protocoles de purification incorporant des opérations de récupération de récolte comprenant une étape de centrifugation à empilement de disques de décharge de solides continus suivies d'étapes de floculation et de filtration en profondeur. Les procédés de récolte de protéines de la divulgation récupèrent des rendements élevés de protéine cible purifiée à partir de cultures en perfusion présentant un volume de cellules condensées supérieur ou égal à 16 %, à l'aide d'un processus non conventionnel mais efficace.
PCT/US2023/032823 2022-09-16 2023-09-15 Procédé de recolte de produits à partir de cultures en perfusion WO2024059235A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263407437P 2022-09-16 2022-09-16
US63/407,437 2022-09-16

Publications (2)

Publication Number Publication Date
WO2024059235A2 WO2024059235A2 (fr) 2024-03-21
WO2024059235A3 true WO2024059235A3 (fr) 2024-05-16

Family

ID=88373895

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/032823 WO2024059235A2 (fr) 2022-09-16 2023-09-15 Procédé de recolte de produits à partir de cultures en perfusion

Country Status (1)

Country Link
WO (1) WO2024059235A2 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012051147A1 (fr) * 2010-10-11 2012-04-19 Abbott Laboratories Procédés de purification de protéines
US9085753B2 (en) * 2008-09-24 2015-07-21 Medimmune, Llc Methods for cultivating cells, propagating and purifying viruses
US9371554B2 (en) * 2011-12-15 2016-06-21 Amgen Inc. Flocculation method
CN106565844A (zh) * 2016-11-08 2017-04-19 深圳万乐药业有限公司 深层过滤单克隆抗体细胞培养液的方法
US20170114381A1 (en) * 2014-06-04 2017-04-27 Amgen Inc. Methods for harvesting mammalian cell cultures
WO2021150996A1 (fr) * 2020-01-24 2021-07-29 Amgen Inc. Procédés de récolte de biomolécules

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319494B1 (en) 1990-12-14 2001-11-20 Cell Genesys, Inc. Chimeric chains for receptor-associated signal transduction pathways
IL104570A0 (en) 1992-03-18 1993-05-13 Yeda Res & Dev Chimeric genes and cells transformed therewith
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
CN102065870B (zh) 2008-04-16 2014-11-19 比奥根艾迪克Ma公司 使用聚亚烷基二醇和过渡金属分离生物大分子的方法
WO2010151632A1 (fr) 2009-06-25 2010-12-29 Bristol-Myers Squibb Company Purification de protéines par précipitation de l'acide caprylique (l'acide octanoïque)
US9133493B2 (en) 2011-04-21 2015-09-15 Amgen Inc. Method for culturing mammalian cells to improve recombinant protein production
PT2837680T (pt) 2011-07-01 2020-04-17 Amgen Inc Cultura celular de mamífero
TW202043253A (zh) 2019-01-28 2020-12-01 美商安進公司 藉由將藥物物質和藥物產品過程整體化的生物製劑製造之連續製造過程
MA55371A (fr) 2019-03-19 2022-01-26 Amgen Inc Détergents alternatifs pour inactivation virale

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9085753B2 (en) * 2008-09-24 2015-07-21 Medimmune, Llc Methods for cultivating cells, propagating and purifying viruses
WO2012051147A1 (fr) * 2010-10-11 2012-04-19 Abbott Laboratories Procédés de purification de protéines
US9371554B2 (en) * 2011-12-15 2016-06-21 Amgen Inc. Flocculation method
US20170114381A1 (en) * 2014-06-04 2017-04-27 Amgen Inc. Methods for harvesting mammalian cell cultures
CN106565844A (zh) * 2016-11-08 2017-04-19 深圳万乐药业有限公司 深层过滤单克隆抗体细胞培养液的方法
WO2021150996A1 (fr) * 2020-01-24 2021-07-29 Amgen Inc. Procédés de récolte de biomolécules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CATALDO A L ET AL: "Economics and ecology: Modelling of continuous primary recovery and capture scenarios for recombinant antibody production", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 308, 4 December 2019 (2019-12-04), pages 87 - 95, XP086032527, ISSN: 0168-1656, [retrieved on 20191204], DOI: 10.1016/J.JBIOTEC.2019.12.001 *

Also Published As

Publication number Publication date
WO2024059235A2 (fr) 2024-03-21

Similar Documents

Publication Publication Date Title
Maddox Production of n-butanol from whey filtrate using Clostridium acetobutylicum NCIB 2951
EP2014760A1 (fr) Méthode pour produire un biopolymère (e.g. un polypeptide) pendant un procédé de fermentation continue
WO2006073846A3 (fr) Procedes pour l'expression et la purification d'hormone de croissance humaine recombinante
JP2017521084A (ja) 微細藻類バイオマスから可溶性タンパク質を抽出する方法
US11193148B2 (en) Method for extracting alpha-ketoglutarate and pyruvate simultaneously from microbial fermentation broth or enzymatic conversion solution
IE51858B1 (en) Extraction of interferon from bacteria
JP7268716B2 (ja) エルゴチオネインの製造方法
WO2024059235A3 (fr) Procédé de recolte de produits à partir de cultures en perfusion
US20090061491A1 (en) Novel fermentation method
JP2017521451A (ja) 微細藻類バイオマスから可溶性タンパク質を抽出するための方法
AU2018299607B2 (en) Method Of Recovering Phosphoric Acid From Fermentation Broth Or Fermentation Waste Liquid And Reusing The Same
US8883968B2 (en) Method for producing surfactin and salt thereof
CN110029091B (zh) Pd-1免疫检查点解除抑制的t淋巴细胞制剂的制法
JPH105501A (ja) 発酵中に生産される蒸留残渣からの有用な化合物の回収方法
KR20010049918A (ko) 고순도 자일리톨의 제조 방법
BG63086B1 (bg) Метод за изолиране на клавуланова киселина от ферментационна хранителна среда чрез ултрафилтруване
RU2230325C2 (ru) Способ приготовления очищенного препарата ботулинического токсина типа а
WO2020173453A1 (fr) Procédé de résolution d'isomère optique par technique d'électrodialyse
RU2322504C1 (ru) Способ получения генно-инженерного инсулина человека
WO2007140274A1 (fr) Préparations de plasmide d'adn et leurs méthodes d'obtention
CN114540403B (zh) 酯化虾青素的制造方法及酯化基因的应用
CN113004137B (zh) 一种降血糖降血脂聚酮类化合物及其制备方法和用途
JP2001017194A (ja) モノヒドロキシアダマンタン誘導体の製造方法
JP4617464B2 (ja) スパイロソームの分離精製方法
RU2237719C2 (ru) Способ получения липополисахаридов

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23789411

Country of ref document: EP

Kind code of ref document: A2