WO2023199574A1 - 培養装置、及び、培養装置の殺菌方法 - Google Patents

培養装置、及び、培養装置の殺菌方法 Download PDF

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Publication number
WO2023199574A1
WO2023199574A1 PCT/JP2023/003199 JP2023003199W WO2023199574A1 WO 2023199574 A1 WO2023199574 A1 WO 2023199574A1 JP 2023003199 W JP2023003199 W JP 2023003199W WO 2023199574 A1 WO2023199574 A1 WO 2023199574A1
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WO
WIPO (PCT)
Prior art keywords
liquid
water
culture
sterilizing
liquid receiving
Prior art date
Application number
PCT/JP2023/003199
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English (en)
French (fr)
Japanese (ja)
Inventor
圭織 吉田
章博 太田
春花 天満
嗣正 樋富
Original Assignee
Ｐｈｃホールディングス株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ｐｈｃホールディングス株式会社 filed Critical Ｐｈｃホールディングス株式会社
Priority to EP23787997.8A priority Critical patent/EP4473981A1/en
Priority to JP2024514810A priority patent/JP7724367B2/ja
Priority to CN202380022601.XA priority patent/CN118715313A/zh
Publication of WO2023199574A1 publication Critical patent/WO2023199574A1/ja
Priority to US18/908,240 priority patent/US20250027026A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10—Apparatus features
    • A61L2202/14—Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10—Apparatus features
    • A61L2202/15—Biocide distribution means, e.g. nozzles, pumps, manifolds, fans, baffles, sprayers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/20—Targets to be treated

Definitions

  • the present disclosure relates to a culture device and a method for sterilizing the culture device.
  • a drawer section is provided below the culture chamber in the culture device described in Patent Document 1.
  • the drawer section is provided with a heater, a tube pump, and a water storage section to humidify the culture chamber. Water stored in the water storage section is dripped onto the heater at predetermined time intervals by a tube pump. The water dropped onto the heater is heated by the heater and becomes steam. The culture chamber is humidified by introducing this steam into the culture chamber through the pipe section.
  • An object of the present disclosure is to provide a culture device and a method for sterilizing the culture device that can appropriately sterilize the flow path of steam and water for steam generation.
  • the culture device includes: A casing having a culture chamber; A steam supply device having a liquid receiving part that receives water, a heater that heats the liquid receiving part to generate steam, and a steam supply pipe that leads the steam to a culture chamber; A water storage part or a sterilizing liquid storage part has a liquid piping configured to be able to be attached selectively, and a liquid feeding pump, and water stored in the water storage part as the liquid feeding pump is driven; or a liquid supply device that supplies the sterilizing liquid contained in the sterilizing liquid storage part to the liquid receiving part; comprising a control device;
  • the control device includes: a cooling process in which water in the water storage section is discharged from the steam supply pipe via the liquid piping and the liquid receiving section without heating the liquid receiving section; After the cooling process, the heater and the liquid feed pump are controlled to perform a sterilization process in which the sterilizing liquid in the sterilizing liquid storage part is discharged from the steam supply pipe via the liquid pipe and the liquid receiving part.
  • the method for sterilizing a culture device includes: A casing having a culture chamber; A steam supply device having a liquid receiving part that receives water, a heater that heats the liquid receiving part to generate steam, and a steam supply pipe that leads the steam to a culture chamber; A water storage part or a sterilizing liquid storage part has a liquid piping configured to be able to be attached selectively, and a liquid feeding pump, and water stored in the water storage part as the liquid feeding pump is driven;
  • a method for sterilizing a culture device comprising: a liquid supply device that supplies a sterilizing solution contained in a sterilizing solution storage section to a liquid receiving section;
  • the culture device is a cooling process of cooling the liquid receiver and the steam supply pipe by discharging the water in the water receiver from the steam supply pipe via the liquid pipe and the liquid receiver without heating the liquid receiver; After the cooling process, the sterilizing liquid in the sterilizing liquid storage part is discharged from the steam supply pipe via the liquid piping and the liquid receiving part, thereby sterilizing the liquid
  • the culture device and the method for sterilizing a culture device of the present disclosure it is possible to appropriately sterilize the flow path of steam and water for steam generation.
  • FIG. 1 is a schematic vertical cross-section of a culture device constituting the culture device viewed from the right side.
  • FIG. 2 is a perspective view of the culture device.
  • FIG. 3 is a schematic diagram showing a connection state between a water tank, a liquid supply device, and a steam supply device.
  • FIG. 4 is a schematic diagram showing a connection state between a dispenser, a liquid supply device, and a vapor supply device.
  • FIG. 5 is a flowchart of the sterilization operation.
  • FIG. 6A is an explanatory diagram of cooling processing in sterilization operation.
  • FIG. 6B is an explanatory diagram of the sterilization process in the sterilization operation.
  • FIG. 6C is an explanatory diagram of replacement processing in sterilization operation.
  • FIG. 1 is a schematic longitudinal section of the inside of the culture device viewed from the right side.
  • FIG. 2 is a perspective view of the culture device.
  • FIG. 3 is a schematic diagram showing a connection state between a water tank, a liquid supply device, and a steam supply device.
  • FIG. 4 is a schematic diagram showing a connection state between a dispenser, a liquid supply device, and a vapor supply device.
  • the front side is the side facing the user of the culture device, and the rear side is the opposite side to the front side.
  • the right side is the right side as seen from the user facing the culture device, and the left side is the opposite side to the right side.
  • the upper side is the upper side when the culture apparatus is installed on a horizontal surface, and the lower side is the opposite side to the upper side.
  • the culture device 1 includes a box-shaped housing 10.
  • a culture chamber 20 for culturing cultured products such as cells or microorganisms is formed inside the casing 10.
  • the culture chamber 20 is configured such that at least one shelf (not shown) can be removably installed at a predetermined position in the vertical direction.
  • the housing 10 includes an inner box 11, an outer box 12, an outer door 13, and an inner door 14.
  • the inner box 11 is formed into a box shape with a culture chamber 20 inside.
  • the opening 21 of the culture chamber 20 is formed in the front surface of the inner box 11.
  • the outer box 12 is formed into a box shape.
  • the outer box 12 is arranged so as to cover parts of the inner box 11 other than the opening 21.
  • a heat insulating material 15 is arranged between the inner box 11 and the outer box 12.
  • the outer door 13 and the inner door 14 open and close the opening 21.
  • a packing P is arranged on the outer edge of the outer door 13.
  • An operating section 50 is arranged on the outer surface of the outer door 13.
  • the operation unit 50 accepts instructions to start, stop, and sterilize the culture apparatus 1, as well as inputs of various setting values for the culture chamber 20.
  • Various set values of the culture chamber 20 include a set temperature, a set humidity, a set concentration of O 2 gas, a set concentration of CO 2 gas, and the like.
  • the operation unit 50 has a display unit that displays the status of the culture device 1.
  • a heating unit 30 that heats the culture chamber 20 is arranged in the housing 10.
  • the heating unit 30 includes a top heater 31 placed above the inner box 11 , a bottom heater 32 placed below the inner box 11 , and a back heater 33 placed on the rear side of the inner box 11 . It includes side heaters (not shown) arranged on the left and right sides of the inner box 11 and an outer door heater 34 arranged on the outer door 13.
  • a duct 22 is arranged in the culture chamber 20.
  • the duct 22 is formed on the rear surface of the inner box 11 so as to extend vertically.
  • a gas passage K is formed inside the duct 22.
  • a circulation blower 23 is arranged in the gas passage K. By operating the circulation blower 23, the air in the culture chamber 20 is sucked in through the suction port 22a formed at the top of the duct 22, and this air enters the culture room 20 through the blowout port 22b provided at the bottom of the duct 22. It's blown out. This causes forced circulation of air as indicated by the thick arrows.
  • Indoor temperature sensor 24 detects the temperature of culture chamber 20 .
  • the indoor temperature sensor 24 is arranged near the suction port 22a and detects the temperature of the air sucked in from the suction port 22a.
  • the gas supply devices 25a and 25b supply adjustment gas (for example, CO 2 gas, O 2 gas, and N 2 (nitrogen) gas) to the cultivation chamber 20 to adjust the O 2 gas concentration and CO 2 gas concentration in the cultivation chamber 20. supply
  • adjustment gas for example, CO 2 gas, O 2 gas, and N 2 (nitrogen) gas
  • a humidifying tray D that stores humidifying liquid (specifically, water) is installed between the lower part of the duct 22 and the bottom surface of the inner box 11.
  • the water stored in the humidifying dish D is sterilized by being irradiated with ultraviolet light from a UV-LED (not shown).
  • a humidity sensor (not shown) is arranged outside the duct 22 to detect the humidity in the culture chamber 20.
  • the back and bottom surface of the outer box 12 of the housing 10 are covered with a cover 16.
  • the space between the back surface of the outer box 12 and the cover 16 forms a machine room M in which various devices are placed.
  • the machine room M is provided with an electrical equipment box 16a.
  • a control device 40 is housed in the electrical equipment box 16a.
  • the culture device 1 further includes an outside temperature sensor 17 and a steam supply device 18.
  • the outside temperature sensor 17 detects the temperature around the culture device 1 .
  • the steam supply device 18 supplies steam to the culture chamber 20.
  • the steam supply device 18 includes a steam generation section 18a and a steam supply pipe 18b.
  • the steam generating section 18a is arranged in the electrical equipment box 16a.
  • the steam supply pipe 18b supplies the steam generated by the steam generation section 18a to the culture chamber 20.
  • a tank storage section 60 is provided on the left side of the outer box 12 of the housing 10.
  • the tank accommodating portion 60 is configured such that a drawer 61 can be taken in and out in the front-back direction.
  • FIGS. 3 and 4 are diagrams illustrating the connection state of each component, and are not diagrams that accurately depict the vertical, front-back, and left-right positional relationships of each component.
  • a water tank 26 a steam supply device 18, a liquid supply device 27, and a humidifying dish D are used.
  • the water tank 26 is an example of a water storage section of the present disclosure that stores water W.
  • the water tank 26 is stored in the drawer 61 in a fixed state.
  • the steam generating section 18a of the steam supply device 18 includes a liquid receiving section 18c, a lid 18d, and a heater 18e.
  • the liquid receiving portion 18c is formed into a block shape from a material having heat conductivity.
  • the liquid receiving portion 18c includes a recessed portion 18c1 recessed downward.
  • a steam supply pipe 18b is inserted through the front side of the liquid receiving portion 18c.
  • the steam supply pipe 18b is inserted so that its inside communicates with the inside of the recess 18c1, and the opening exposed to the recess 18c1 is located above the bottom surface of the recess 18c1.
  • the lid 18d closes the upper surface of the liquid receiving portion 18c.
  • the heater 18e is arranged near the recess 18c1 in the liquid receiving portion 18c.
  • the heater 18e is electrically connected to the control device 40.
  • the heater 18e generates steam by heating and evaporating the water supplied to the concave portion 18c1 of the liquid receiving portion 18c under the control of the control device 40.
  • the culture chamber 20 is humidified by introducing this steam into the culture chamber 20 via the steam supply pipe 18b.
  • the liquid supply device 27 includes a liquid tube 27a and a tube pump 27b.
  • the liquid tube 27a is a liquid pipe according to the present disclosure, and is made of resin or rubber. A portion of liquid tube 27a is located within tube pump 27b. One end of the liquid tube 27a is attached to the lid 18d of the liquid receiver 18c so that the inside of the liquid tube 27a and the inside of the recess 18c1 of the liquid receiver 18c communicate with each other. As shown in FIG. 3, the other end of the liquid tube 27a is provided with a tube connection port 27c for guiding the water W in the water tank 26 to the liquid receiving portion 18c.
  • the cap 26a of the water tank 26 is provided with a cap connection port 26b. The upper portion of the cap connection port 26b is configured to be able to connect the tube connection port 27c.
  • a tank tube 26c is attached to a lower portion of the cap connection port 26b. A portion of the tank tube 26c is located in the water W.
  • the tube connection port 27c of the liquid tube 27a is configured such that the discharge portion 28a of the dispenser 28 can be inserted in order to guide the ethanol E in the dispenser 28 to the liquid receiving portion 18c.
  • the liquid tube 27a is configured so that either the water tank 26 or the dispenser 28 can be attached thereto.
  • the tube connection port 27c is configured with a male Luer Lock standard connector
  • the cap connection port 26b and the dispenser discharge portion 28a are configured with female Luer Lock standard connectors.
  • the liquid tube 27a , a water tank 26, or a dispenser 28 can be attached alternatively.
  • the shapes of the tube connection port 27c, the cap connection port 26b, and the discharge portion 28a may have the male and female shapes reversed. In this case, there is an advantage that it can be connected to a general-purpose dispenser having a lure shape such as a general syringe.
  • the tube pump 27b is an example of the liquid feeding pump of the present disclosure.
  • Tube pump 27b is electrically connected to control device 40.
  • the tube pump 27b pumps water W in the water tank 26 or ethanol E in the dispenser 28 by rotating a rotor (not shown) that crushes a part of the liquid tube 27a under the control of the control device 40.
  • the liquid is supplied to the liquid receiving portion 18c.
  • the tube pump 27b is configured to be able to adjust the amount of water W supplied to the liquid receiving portion 18c per unit time by increasing or decreasing the rotational speed of the rotor.
  • the dispenser 28 is an example of the sterilizing liquid storage unit of the present disclosure.
  • the ethanol E contained in the dispenser 28 is an example of the sterilizing liquid of the present disclosure.
  • the dispenser 28 is sized so that it can be placed in the drawer 61 instead of the water tank 26.
  • the dispenser 28 includes a syringe 28b having a discharge portion 28a, and a piston 28c that reciprocates within the syringe 28b.
  • a scale (not shown) indicating the amount of ethanol E in the syringe 28b is displayed on the syringe 28b. In this manner, by using the dispenser 28 with the scale displayed, the user can fill the dispenser 28 with the minimum amount of ethanol E to be used.
  • the capacity of the dispenser 28 is 10 ml or less in order to suppress the use of the liquid.
  • the tube pump 27b supplies the ethanol E in the dispenser 28 to the liquid receiving portion 18c, the tube pump 27b sends the ethanol E while retracting the piston 28c.
  • the capacity of the dispenser 28 is 20 ml or less.
  • ⁇ Culture operation> First, as an operation of the culture apparatus 1, a culture operation for culturing a culture will be described.
  • the user installs the humidifying dish D in the culture chamber 20 and places, for example, a petri dish containing the culture on a shelf.
  • the control device 40 controls the inside of the casing 10 (culture chamber 20) to the concentration and temperature of each gas suitable for culturing the culture.
  • the circulation blower 23, the gas supply devices 25a and 25b, and the heating unit 30 are controlled so that the temperature increases.
  • the bottom heater 32 generates heat, the water stored in the humidifying dish D evaporates, and the culture chamber 20 is humidified so that the humidity in the culture chamber 20 reaches the target value.
  • the control device 40 selects either normal humidification control or rapid humidification control based on information input by the user to the operation unit 50, and the humidity detected by the humidity sensor becomes the target value.
  • the normal humidification control is a control in which the steam supply device 18 is not operated, and the culture chamber 20 is humidified by evaporating the water in the humidifying dish D by the heat generated by the bottom heater 32.
  • the rapid humidification control is a control that evaporates the water in the humidifying dish D by the heat generated by the bottom heater 32, and also humidifies the culture chamber 20 by operating the steam supply device 18. Humidification can be done early.
  • the control device 40 drives the heater 18e of the steam supply device 18 to heat the liquid receiving portion 18c, and also drives the tube pump 27b of the liquid supply device 27 to heat the water W in the water tank 26.
  • the liquid is supplied to the liquid receiving portion 18c.
  • the control device 40 intermittently drives the tube pump 27b so as to drop the water W into the liquid receiving portion 18c at predetermined intervals. Steam is generated from the liquid receiving portion 18c into which water W has been dropped, and the steam is guided to the culture chamber 20, thereby humidifying the culture chamber 20.
  • the control device 40 stops the circulation blower 23, the gas supply devices 25a and 25b, and the heating section 30, and starts the steam supply device 18. If so, the steam supply device 18 is stopped.
  • FIG. 5 is a flowchart of the sterilization operation.
  • FIG. 6A is an explanatory diagram of cooling processing in sterilization operation.
  • FIG. 6B is an explanatory diagram of the sterilization process in the sterilization operation.
  • FIG. 6C is an explanatory diagram of replacement processing in sterilization operation.
  • the control device 40 causes the operation unit 50 to display a setup instruction.
  • the setup instruction includes, for example, an instruction to place the humidifying dish D below the steam supply pipe 18b and an instruction to check the amount of water in the water tank 26.
  • the setup instruction includes, for example, an instruction to place the humidifying dish D below the steam supply pipe 18b and an instruction to check the amount of water in the water tank 26.
  • the user since water W and ethanol E are discharged into the humidifying tray D, it is preferable for the user to empty the humidifying tray D based on the setup instructions.
  • the user presses a setup completion button displayed on the operation unit 50.
  • an instruction to evacuate the culture (not shown) from the culture chamber 20 may also be displayed. This prevents the user from forgetting to save the culture and prevents the culture from dying unintentionally.
  • control device 40 When the control device 40 detects the operation of the setup completion button, it starts the cooling process as shown in FIG. 5 (step S1).
  • the control device 40 drives the tube pump 27b to supply water W in the water tank 26 to the liquid receiving portion 18c.
  • the control device 40 controls the tube pump 27b to continuously supply water W to the liquid receiving portion 18c. That is, the control device 40 controls the tube pump 27b so that the amount of water W supplied to the liquid receiving portion 18c per unit time is greater than when performing rapid humidification control. By controlling the amount of water in this manner, the temperature of the liquid receiving portion 18c can be quickly lowered.
  • the control device 40 may display the temperature of the liquid receiving portion 18c on the operation unit 50 during the cooling process. After a predetermined period of time has passed since the start of the cooling process, the control device 40 controls the tube pump 27b to end the supply of water W to the liquid receiving portion 18c, thereby ending the cooling process. Note that the control device 40 may terminate the cooling process based on the user's operation of the operation unit 50, or may indicate that the temperature of the liquid receiving portion 18c and the steam supply pipe 18b has become below the first predetermined temperature. When detected, the cooling process may be terminated.
  • the first predetermined temperature is 100° C. or lower at which the supplied water W does not boil in the liquid receiving portion 18c.
  • This cooling process cools down the liquid receiving portion 18c and the steam supply pipe 18b, which have become high in temperature due to the culture operation.
  • the vapor supply device 18 includes an evaporating section temperature sensor (not shown) that measures the temperature of the liquid receiving section 18c, and the vapor supplying device 18 is equipped with an evaporating section temperature sensor (not shown) that measures the temperature of the liquid receiving section 18c. Water W is supplied to the section 18c. That is, after confirming that the temperature of the liquid receiving part 18c has decreased sufficiently, the supply of water W to the liquid receiving part 18c is ended.
  • the control device 40 causes the operation unit 50 to display a first liquid replacement instruction to replace the water tank 26 with the dispenser 28.
  • the user pulls out the drawer 61, pulls out the tube connection port 27c of the liquid tube 27a from the cap connection port 26b of the water tank 26, and pulls out the water tank 26. Take it out.
  • the user inserts the discharge part 28a of the dispenser 28 filled with the set amount of ethanol E into the tube connection port 27c of the liquid tube 27a.
  • the dispenser 28 is no longer fixed in the drawer 61 and is suspended from the liquid tube 27a.
  • the user pushes the drawer 61 into the tank housing 60 along with the dispenser 28.
  • the user presses the first liquid replacement completion button displayed on the operation unit 50.
  • control device 40 When the control device 40 detects the operation of the first liquid exchange completion button, it starts the sterilization process as shown in FIG. 5 (step S2). In the sterilization process, the control device 40 drives the tube pump 27b to supply the ethanol E in the dispenser 28 to the liquid receiving portion 18c. At this time, the control device 40 controls the tube pump 27b to continuously supply ethanol E to the liquid receiving portion 18c, similarly to the cooling process.
  • the ethanol E supplied to the liquid receiving portion 18c accumulates in the recess 18c1, and is eventually discharged to the humidifying dish D via the steam supply pipe 18b.
  • the control device 40 stops the tube pump 27b and ends the sterilization process.
  • the control device 40 may terminate the sterilization process with ethanol E remaining in the dispenser 28 after a predetermined period of time has elapsed from the start of the sterilization process, or may terminate the sterilization process based on the user's operation of the operation unit 50. , the sterilization process may be completed.
  • the liquid tube 27a, the liquid receiving portion 18c, and the steam supply pipe 18b are sterilized with ethanol E.
  • the control device 40 causes the operation unit 50 to display a second liquid replacement instruction to replace the dispenser 28 with the water tank 26.
  • the user pulls out the discharge part 28a of the dispenser 28 from the tube connection port 27c of the liquid tube 27a, and stores the water tank 26 containing water W in the drawer 61.
  • the user inserts the liquid tube 27a into the tube connection port 27c of the water tank 26 into the cap connection port 26b, and pushes the drawer 61 into the tank storage section 60.
  • the user presses the second liquid exchange completion button displayed on the operation unit 50.
  • control device 40 When the control device 40 detects the operation of the second liquid replacement completion button, it starts the replacement process as shown in FIG. 5 (step S3). In the replacement process, the control device 40 controls the tube pump 27b to continuously supply water W to the liquid receiving part 18c, similarly to the cooling process.
  • the control device 40 controls the tube pump 27b to end the supply of water W to the liquid receiving portion 18c, thereby ending the replacement process.
  • the control device 40 may end the replacement process based on the user's operation of the operation unit 50. Through such a replacement process, the ethanol E adhering to the liquid tube 27a, liquid receiving portion 18c, and steam supply pipe 18b is replaced with water W.
  • the control device 40 starts the evaporation process, as shown in FIG. 5 (step S4).
  • the control device 40 controls the heater 18e of the vapor supply device 18 to heat the liquid receiving portion 18c.
  • the control device 40 stops the heater 18e after a predetermined time has elapsed from the start of the evaporation process, or when the temperature of the liquid receiving portion 18c reaches a second predetermined temperature or higher, and ends the evaporation process.
  • the second predetermined temperature is desirably 100° C. or higher at which the water W in the liquid receiving portion 18c reliably evaporates. Through such evaporation processing, the water W in the heated liquid receiving portion 18c is evaporated and discharged into the culture chamber 20 via the steam supply pipe 18b.
  • the water W adhering to the steam supply pipe 18b is also evaporated and discharged into the culture chamber 20.
  • the evaporation process is continued in which the temperature of the liquid receiving portion 18c is maintained at 100° C. or higher for 1 minute or more. That is, the evaporation process is finished after confirming that the water W in the liquid receiving portion 18c has been sufficiently evaporated.
  • the culture operation it is possible to prevent the water W remaining in the liquid receiving portion 18c, which may contain ethanol E, from evaporating and affecting the culture.
  • control device 40 causes the operation unit 50 to display a sterilization operation end screen.
  • the user discards the liquid (water W and ethanol E) in the humidifying dish D and returns it to the culture chamber 20.
  • the control device 40 lowers the temperature of the liquid receiving portion 18c by the cooling process using water W, and then performs the sterilization process using ethanol E. Therefore, even if a sterilization operation is performed immediately after a culture operation, by supplying ethanol E to the liquid receiving portion 18c, which has a low temperature, the flow path of steam and water for steam generation can be safely sterilized. I can do it.
  • the temperature of the liquid receiving portion 18c is forcibly lowered by the cooling process, sterilization can be performed in a shorter time than in the case where the sterilization process is performed after leaving the liquid receiving part 18c until the temperature drops. Therefore, it is possible to appropriately sterilize the flow path of steam and water for steam generation.
  • the control device 40 performs a replacement process in which ethanol E adhering to the liquid tube 27a, liquid receiving portion 18c, and steam supply pipe 18b is replaced with water W. Therefore, it is possible to prevent the culture operation from being performed while the components of ethanol E remain in the flow path of the steam and the water for steam generation, and it is possible to appropriately culture the culture product. In addition, the culture operation can be started early without waiting until ethanol E naturally evaporates.
  • the control device 40 performs an evaporation process to evaporate the water W adhering to the liquid receiving part 18c and the steam supply pipe 18b. Therefore, the culture operation can be started without the water W used in the sterilization operation remaining in the liquid receiving portion 18c and the steam supply pipe 18b. Furthermore, the culture operation can be started early without waiting until the water W adhering to the liquid receiving portion 18c and the steam supply pipe 18b is naturally dried.
  • the evaporation process may not be performed, and in this case, it is preferable to wait until the liquid receiving part 18c and the steam supply pipe 18b are naturally dried before starting the culture operation.
  • it is not necessary to perform the replacement process and the evaporation process and in this case, wait until the ethanol E attached to the liquid tube 27a, the liquid receiving part 18c, and the steam supply pipe 18b evaporates naturally, and then start the culture. It is preferable to start.
  • the amount of water W supplied per unit time to the liquid receiving portion 18c when performing the replacement process may be the same as when performing rapid humidification control.
  • tube pump 27b is shown as an example of the liquid feeding pump, any pump capable of feeding liquid such as a diaphragm type, piston type, or plunger type may be applied.
  • a configuration other than the dispenser 28, such as a tank having the same shape as the water tank 26, may be applied as the sterilizing liquid storage section, and a configuration other than the water tank 26, such as a dispenser, may be applied as the water storage section.
  • Ethanol E is used as an example of the sterilizing liquid, but a liquid having a sterilizing effect such as IPA (Isopropyl Alcohol) may also be used.
  • IPA Isopropyl Alcohol
  • the present disclosure can be applied to a culture device and a method for sterilizing a culture device.

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PCT/JP2023/003199 2022-04-15 2023-02-01 培養装置、及び、培養装置の殺菌方法 WO2023199574A1 (ja)

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EP23787997.8A EP4473981A1 (en) 2022-04-15 2023-02-01 Culturing device and method for sterilizing culturing device
JP2024514810A JP7724367B2 (ja) 2022-04-15 2023-02-01 培養装置、及び、培養装置の殺菌方法
CN202380022601.XA CN118715313A (zh) 2022-04-15 2023-02-01 培养装置及培养装置的杀菌方法
US18/908,240 US20250027026A1 (en) 2022-04-15 2024-10-07 Culturing apparatus and method for sterilizing culturing apparatus

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006320486A (ja) * 2005-05-18 2006-11-30 Earekkusu:Kk 除染システム、及び除染方法
JP2017201886A (ja) * 2016-05-09 2017-11-16 株式会社エアレックス インキュベータ
JP2021078437A (ja) 2019-11-20 2021-05-27 Ｐｈｃホールディングス株式会社 培養装置
JP2022067630A (ja) 2020-10-20 2022-05-06 コスモ石油株式会社 石油精製プロセスにおける原料油予熱用熱交換器の汚れ防止剤、石油精製プロセス用原料油および石油精製プロセスにおける原料油予熱用熱交換器の汚れ防止方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0728722B2 (ja) * 1990-06-29 1995-04-05 富士写真フイルム株式会社 バイオリアクター装置
JPH057614A (ja) * 1990-08-13 1993-01-19 Kimura Chem Plants Co Ltd 連続加熱滅菌装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006320486A (ja) * 2005-05-18 2006-11-30 Earekkusu:Kk 除染システム、及び除染方法
JP2017201886A (ja) * 2016-05-09 2017-11-16 株式会社エアレックス インキュベータ
JP2021078437A (ja) 2019-11-20 2021-05-27 Ｐｈｃホールディングス株式会社 培養装置
JP2022067630A (ja) 2020-10-20 2022-05-06 コスモ石油株式会社 石油精製プロセスにおける原料油予熱用熱交換器の汚れ防止剤、石油精製プロセス用原料油および石油精製プロセスにおける原料油予熱用熱交換器の汚れ防止方法

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US20250027026A1 (en) 2025-01-23
JP7724367B2 (ja) 2025-08-15
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CN118715313A (zh) 2024-09-27

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