WO2023082121A1 - Burdock nutritional grain meal replacement powder and preparation method therefor - Google Patents

Abstract

A burdock nutritional cereal meal replacement powder, comprising the following components in parts by weight: 20-50 parts by weight burdock powder, 10-20 parts by weight soybean powder, 5-15 parts by weight whey protein powder, 1-10 parts by weight sesame, 3-6 parts by weight tremella powder, 6-12 parts by weight Chinese wolfberry powder, 9-15 parts by weight cooked polygonatum powder, 5-15 parts by weight adzuki beans, 1-5 parts by weight oats, 1-5 parts by weight quinoa, 6-12 parts by weight Polygonatum odoratum powder, 0.001-0.005 parts by weight iron, 0-1 parts by weight sodium, 0.0002-0.0005 parts by weight vitamin A, and 0.01-0.08 parts by weight taurine. The burdock powder is burdock root having undergone modification by steam explosion and synergistic fermentation, and burdock nano dietary fiber powder is prepared using nanocrystallization processing. The present invention further relates to a preparation method for the burdock nutrition grain meal replacement powder, and the obtained burdock nutrition cereal meal replacement powder can adjust diversity of intestinal strains and lower blood sugar.

Description

A kind of burdock nutritional cereal meal replacement powder and preparation method thereof technical field

The invention relates to the technical field of hydrogel preparation, in particular to a burdock nutritional cereal meal replacement powder and a preparation method thereof.

Background technique

The prevention and treatment of chronic metabolic diseases, especially the prevention and treatment of diabetes, is mainly carried out based on drug therapy combined with changes in daily life habits. At present, the intervention of type 2 diabetes and the regulation of blood sugar are mainly drug treatment, which basically has certain side effects on the body and may even produce a series of adverse reactions, which is not conducive to the health of users. At present, there are certain hypoglycemic related products on the market, such as some meal replacement products, tea drinks and other product types. Among them, meal replacement products with low sugar, high protein and added nutritional elements can meet the daily needs of the human body for protein and control glucose, but they are often conceptual products with poor solubility, unreasonable compatibility, and lack of hypoglycemic effect. Persistence and ensure the stability of postprandial blood sugar, even nutrition imbalance, poor satiety effect; and fruit meal replacement, vegetable meal replacement not only lack of protein, but also lack of many minerals and trace elements, long-term, single Eating not only fails to achieve the expected effect of lowering blood sugar, but also causes nutritional imbalance in the body, causing health problems such as pernicious anemia, protein deficiency, and excessive muscle consumption; The elevated phenomenon has not been improved. A balanced diet pattern is the basis for ensuring the nutritional needs and health of the human body to the greatest extent. However, there is a lack of effective measures aimed at the common problems of chronic diseases and the development of physiological and pathological processes.

Meal replacement powder is a supplementary food that partially replaces the daily diet that appears with the increase in obesity, hyperglycemia, and hyperlipidemia. It is mainly used in the fields of diabetes and other chronic diseases. It combines the advantages of convenient eating, strong satiety, and balanced nutrition. However, many nutritional meal replacement powders often have problems such as nutritional imbalance and unreasonable compatibility, which cannot effectively improve consumption. Patients with abnormal blood sugar.

Burdock is a biennial herb of the genus Burdock in the Compositae family. It is commonly known as "Japanese Ginseng" and has the reputation of "King of Vegetables". In 2002, burdock was included in the national new resource food, and was praised as "one of the best health foods for human beings in the 21st century" by the Food and Agriculture Organization of the United Nations (FAO). Burdock root contains inulin, volatile oil, polysaccharides, phenols and aldehydes, and is rich in cellulose and amino acids. The content of carotene is 150 times higher than that of carrots, and the content of protein and calcium is the first in rhizomes. Burdock root is a It can be suitable as a natural raw material for hypoglycemic meal replacement powder. At present, the problem to be solved urgently in this field is: how to maximize the efficacy of burdock, and solve the problem that the hypoglycemic series products on the market are not obvious in regulating blood sugar or the blood sugar is not stable after meals.

Contents of the invention

It is an object of the present invention to solve at least the above-mentioned problems and to provide at least the advantages which will be described later.

Another object of the present invention is to provide a burdock nutritious cereal meal replacement powder, which uses burdock as the main raw material, and through the synergistic effect between the nutrients in the formula, it can synergistically inhibit the rise of blood sugar levels after meals and increase insulin sensitivity; safe Reliable and effective in regulating blood sugar.

In order to achieve these objects and other advantages according to the present invention, a kind of burdock nutritional cereal meal replacement powder is provided, which includes the following components by weight: 20-50 parts by weight of burdock powder, 10-20 parts by weight of soybean powder , 5-15 parts by weight of whey protein powder, 1-10 parts by weight of sesame, 3-6 parts by weight of white fungus powder, 6-12 parts by weight of wolfberry powder, 9-15 parts by weight of cooked rhizome powder, 5-15 parts by weight of red bean , 1-5 parts by weight of oats, 1-5 parts by weight of quinoa, 6-12 parts by weight of Yuzhu powder, 0.001-0.005 parts by weight of iron, 0-1 parts by weight of sodium, 0.0002-0.0005 parts by weight of vitamin A and taurine 0.01-0.08 parts by weight; the energy provided by the burdock nutritional cereal meal replacement powder of the present invention is about 360Kcal, which meets the group standard T/CNSS002-2019 for meal replacement foods. Based on the requirement of 400Kcal. The nutritional additive taurine in the meal replacement powder product burdock nutritional cereal meal replacement powder meets the range of 1.1g/kg-1.4g/kg taurine in the GB14880 solid beverage standard.

Preferably, the burdock nutritional cereal meal replacement powder includes the following ingredients in parts by weight: 30 parts by weight of burdock powder, 10 parts by weight of soybean powder, 10 parts by weight of whey protein powder, 5 parts by weight of sesame, 5 parts by weight of white fungus powder Parts by weight, 10 parts by weight of wolfberry powder, 10 parts by weight of cooked Polygonatum powder, 5 parts by weight of red bean, 4 parts by weight of oats, 5 parts by weight of quinoa, 6 parts by weight of Polygonatum odoratum powder, 0.002 parts by weight of iron, 0.4 parts by weight of sodium, 0.000275 parts by weight of vitamin A and 0.055 parts by weight of taurine.

Preferably, the burdock powder is burdock nano-dietary fiber powder prepared from burdock roots modified by steam explosion cooperative fermentation and nano-processing.

The present invention also provides a preparation method of the burdock nutritional cereal meal replacement powder, which comprises the following steps:

Step 1, preparing burdock nano-dietary fiber powder, specifically comprising:

The cleaned burdock root slices in step 1.1 are pretreated by steam explosion technology under 1.2MPa/90s steam explosion conditions to obtain modified burdock root slices;

Step 1.2 Fermentation modification: Dry the modified burdock root slices prepared in step 1 and ultrafinely pulverize them through a 200-mesh sieve, add distilled water at a material-to-liquid ratio of 1:10-15 to obtain a homogenate of burdock root powder. 2-6% of the starter is added, fermented at 38°C-45°C for 2-3 days, sterilized and freeze-dried to obtain modified burdock powder;

Step 1.3 Nano-processing: the modified burdock powder is subjected to step-by-step enzymatic decontamination at room temperature to obtain burdock insoluble dietary fiber, after the burdock insoluble dietary fiber is mixed with ultrapure water, cellulase is added, and the Process under high-pressure micro-fluidic conditions, centrifuge and freeze-dry to obtain burdock nano dietary fiber powder; the burdock insoluble dietary fiber enhances water holding capacity, oil holding capacity and expansion force through steam explosion technology, and enhances the resistance to α-glucosidase inhibition. The insoluble dietary fiber of burdock root obtained by steam explosion technology is further enhanced by fermentation method to enhance water holding capacity and expansion force. Under the observation of microstructure, it has more wrinkled structure, enhanced specific surface area, further increases the combination with α-glucosidase, and inhibits α-glucosidase. Glucosidase activity.

Step 2, preparation of plant raw material powder: grind the washed and dried soybeans, sesame, white fungus, cooked Polygonatum, medlar, quinoa, oats, red bean and Yuzhu pulverizer to 200-325 mesh, according to the weight ratio Mix evenly to prepare plant raw material mixed powder.

Step 3, irradiation sterilization: the burdock nano-dietary fiber powder and the mixed plant raw material powder are irradiated and sterilized, the irradiation source is Co-60, and the irradiation dose is 5-8kGy.

Step 4, deployment: mix burdock nano-dietary fiber powder, plant raw material mixed powder, whey protein powder, iron, sodium, vitamins and taurine in proportion, and dry until the water content of the product is 8-15%, to obtain burdock nutritional cereal substitute meal powder.

Preferably, in the step 1, the burdock root slices are prepared through the following steps: after cleaning the burdock root with chlorine dioxide spray, cut into slices with a thickness of 6 mm, and then blanched in boiling water or soaked in 1.0% lemon Prepared after acid protectant.

Preferably, the starter is prepared through the following steps: select Lactobacillus bulgaricus and Streptococcus thermophilus for inoculation and culture, and when the number of viable bacteria reaches 109-1010, mix the two in a ratio of 1:1 to make a starter . Both Lactobacillus bulgaricus and Streptococcus thermophilus were obtained from the China Industrial Microorganism Culture Collection and Management Center.

Preferably, in the step 1.3, the burdock insoluble dietary fiber is prepared through the following steps: obtain a modified burdock powder homogenate at a ratio of 1:10 to liquid, and press α-amylase, glucoamylase and pineapple respectively at room temperature The sequence of proteases is decontaminated step by step under ultrasonic conditions. After each step of enzymatic decontamination, use a boiling water bath to deactivate the enzyme treatment. Washing with 80% ethanol, centrifuging again, collecting the precipitate, and drying under the condition of 40-50 DEG C to obtain the burdock insoluble dietary fiber.

Preferably, in the step 1.3, the ratio of the burdock insoluble dietary fiber to ultrapure water is 1:10-20; the added amount of the cellulase is 10-20u/g per gram of burdock insoluble dietary fiber Cellulase.

Preferably, in the step 1.2, the burdock powder homogenate needs to be sterilized under high temperature and high pressure at 121° C. for 20 minutes to obtain sterile burdock powder homogenate before fermentation.

Beneficial effect:

Through the study, it was found that burdock root can regulate the diversity of blood sugar-related populations in the intestinal flora, and can better inhibit the activity of α-glucosidase. Burdock root has great potential in regulating blood sugar. The burdock nutritious grain meal replacement powder of the present invention makes full use of the nutrient-rich excellent characteristics of burdock, such as rich dietary fiber (27.2%), low fat (0.7%), and various essential amino acids for human body, and at the same time, it is compatible with a variety of rich nutritional ingredients , Raw materials rich in dietary fiber, such as soybean powder, sesame, red bean, etc., can better meet the energy, vitamins, oils, amino acids, etc. required by the human body. The overall formula not only reasonably controls the sugar intake of the eaters, but also ensures balanced nutrition. , while enhancing satiety.

The burdock nutritious grain meal replacement powder overcomes the disadvantages of common meal replacement powders that are easy to agglomerate, sink to the bottom, and have poor taste through the synergistic effect of nutrients. In addition, on the basis of ensuring nutritional balance, choose cooked sealwort and medlar for compatibility, enhance the effect of regulating blood sugar, and choose Polygonatum odoratum with potential for lowering blood sugar, so that the compatible ingredients for regulating blood sugar are more effective and effective Be innovative. The compatibility of these ingredients has certain positive effects on blood sugar regulation, prevention and treatment of diabetes, and at the same time, it can synergistically inhibit the rise of blood sugar levels after meals and increase insulin sensitivity. Among them, the addition of wolfberry fruit makes the meal replacement powder have a good taste of meal replacement, and is low in calories and easy to accept.

The burdock nutritional cereal meal replacement powder of the present invention proceeds from the perspective of the disorder of glucose metabolism caused by the modern irregular diet environment and the needs of people prone to diabetes, selects burdock rich in dietary fiber and burdock polysaccharide as the main raw material, and extracts the insoluble Dietary fiber is modified to obtain burdock nano-dietary fiber with significant hypoglycemic effect, fully utilize and enhance its ability to delay gastric emptying, delay the digestion rate of starch in the small intestine and inhibit α-glucosidase activity to reduce glucose absorption The role of burdock to maximize the regulation of blood sugar and regulate the stability of postprandial blood sugar. Through the in vivo test of rats and human experiments, it has been determined that the burdock nutritional cereal meal replacement powder of the present invention has an effect on the increase of rat blood sugar level induced by high-calorie diet combined with injection of streptozotocin (STZ) and the blood sugar level of type 2 diabetes crowd. It has a good improvement effect. The burdock nutritious grain meal replacement powder of the present invention has the beneficial effects of balanced nutrition, effective regulation of postprandial blood sugar level, good brewability, no particles, uniform color, delicate taste, moderate viscosity, pure taste, and coordinated smell.

The preparation method of the burdock nutritious grain meal replacement powder described in the present invention is to modify the burdock raw material through the technology of the steam explosion cooperative fermentation method, and the obtained burdock dietary fiber has stronger water holding capacity and expansion force, and can be enhanced by nano-processing. α-glucosidase forms a complex to change the protein structure and inactivate it, which increases the binding ability of burdock insoluble dietary fiber and α-glucosidase, and further inhibits its activity, so that burdock nano-sized dietary fiber can be used as a better regulator Functional food ingredients for blood sugar levels are applied to this meal replacement powder.

The preparation method of the burdock nutritious grain meal replacement powder of the present invention uses Lactobacillus bulgaricus and Streptococcus thermophilus to ferment and modify the burdock dietary fiber, and microbial fermentation modification is a relatively safe, efficient and low-cost dietary fiber modification method. This method improves the specific surface area of the burdock nano dietary fiber, strengthens the combination of the remaining α-glucosidase, further enhances its enzyme inhibitory activity, and makes the burdock nano dietary fiber applied to the burdock nutritional cereal meal replacement powder have the ability to lower blood sugar. More obvious, the suppression of postprandial blood sugar levels is more significant. In addition, it can improve the flavor of burdock and reduce the earthy smell of burdock root fiber.

Other advantages, objectives and features of the present invention will partly be embodied through the following descriptions, and partly will be understood by those skilled in the art through the research and practice of the present invention.

Description of drawings

Fig. 1 shows the electron micrograph of the burdock powder described in the preparation method of the burdock nutrition cereal meal replacement powder described in one of the embodiments of the present invention;

Fig. 2 shows the electron micrograph of the burdock insoluble dietary fiber powder described in the preparation method of the burdock nutritional cereal meal replacement powder described in one of the embodiments of the present invention;

Fig. 3 shows the electron micrograph of the burdock nano dietary fiber powder described in the preparation method of the burdock nutritional cereal meal replacement powder described in one of the embodiments of the present invention;

Fig. 4 shows the impact curve of the content of the burdock nano dietary fiber powder in the burdock nutritional cereal meal replacement powder of the present invention on the viscosity of the burdock nutritional cereal meal replacement powder;

Figure 5 shows the distribution characteristics of different treatments of the present invention based on the family level of the intestinal flora of mice;

Fig. 6 shows that different treatments of the present invention are based on the distribution characteristics of the mouse intestinal flora at the genus level;

Fig. 7 shows the changes in the blood sugar levels of the subjects after the intervention of the burdock nutritional cereal meal replacement powder according to one embodiment of the present invention.

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

The present invention will be further elaborated below in conjunction with embodiment.

Example 1

The preparation method of burdock nutritional cereal meal replacement powder of the present invention comprises the following steps:

S1: Prepare burdock nano dietary fiber powder:

(1) Burdock raw material selection: select large-scale burdock with a diameter of 2.2cm to 2.5cm in the largest part, and remove the necrotic part;

(2) Burdock cleaning: select chlorine dioxide spray cleaning;

(3) Slicing: cut the burdock into slices with a thickness of 6mm, blanched in boiling water or soaked in 1.0% citric acid protective agent for 120s;

(4) Modification of burdock root slices: pretreatment of burdock root slices by steam explosion technology under 1.2MPa/90s steam explosion conditions can better maintain the functional properties and thermodynamic stability of IDF in burdock roots;

(5) Preparation of starter: when the number of live bacteria reaches 1010, Lactobacillus bulgaricus and Streptococcus thermophilus are mixed in a ratio of 1:1 to make starter.

(6) Fermentation modification pretreatment: After drying the modified burdock root slices at 90°C for 4 hours, pass through a 200-mesh sieve through ultrafine pulverization technology, and then add distilled water in a ratio of 1:10-15 to the solid-liquid ratio. Obtain burdock powder homogenate, and then obtain aseptic burdock powder homogenate by sterilizing at 121° C. for 20 minutes under high temperature and high pressure;

(7) Fermentation modification: the burdock powder homogenate is inoculated with a starter for fermentation. Fermented at 40°C for 3 days, sterilized and freeze-dried to obtain modified burdock powder;

(8) Prepare burdock insoluble dietary fiber: the modified burdock powder prepared in step (7) is made into a homogenate at a ratio of 1:10 to liquid, and is prepared in the order of α-amylase, glucoamylase and bromelain at room temperature. Under ultrasonic conditions, step-by-step enzymatic decontamination is carried out. After each step of enzymatic decontamination, the enzyme is deactivated in a boiling water bath. After the decontamination is completed, centrifuge to collect the precipitate, wash it with warm water at 60°C, and then use 80% volume ratio of Washing with ethanol, centrifuging again, collecting the precipitate, and then drying at 40-50°C to obtain burdock insoluble dietary fiber powder;

(9) Nano treatment: fully mix the burdock insoluble dietary fiber powder prepared in step (8) with ultrapure water according to the ratio of 1:10 material to liquid, and add 16u/g of cellulose per gram of insoluble dietary fiber in the feed liquid The enzyme was treated under the condition of 90MPa high-pressure micro-jet, and then centrifuged to remove the precipitate, and the supernatant was collected and freeze-dried to obtain the burdock nano dietary fiber powder modified by steam explosion cooperative fermentation.

S2. Preparation of plant raw material powder: grind the washed and dried soybeans, sesame, cooked Polygonatum, medlar, quinoa, white fungus, oats, red bean and Polygonatum pulverizer to 200 mesh, take 10g of soybean powder, sesame 5g, tremella powder 5g, wolfberry powder 10g, cooked Polygonatum powder 10g, red bean 5g, oat powder 4g, quinoa powder 5g, Polygonatum odoratum powder 6g, mix well to get plant raw material mixed powder.

S3. Irradiation sterilization: the burdock nano-dietary fiber powder and the mixed plant raw material powder are irradiated and sterilized, the irradiation source is Co-60, and the irradiation dose is 5-8kGy.

S4, deployment: according to the recipe, mix 30g of burdock nano-dietary fiber powder, the mixed plant raw material powder prepared in step S2, 2mg of iron, 400mg of sodium, 200ug of vitamin A, 55mg of taurine and 10g of whey protein powder, mix evenly, and dry to the final product The water content is 8-15%, and the burdock nutritious grain meal replacement powder is obtained.

Example 2

Prepare 100 mg of the burdock nutritional cereal meal replacement powder, which is made of the following components: 30 mg of burdock nano dietary fiber powder, 10 mg of soybean powder, 10 mg of whey protein powder, 5 mg of sesame, 5 mg of white fungus powder, 10 mg of wolfberry powder, and cooked Huang Jing powder 10mg, Red Bean 5mg, Oat 4mg, Quinoa 5mg, Polygonatum Polygonatum Powder 6mg, Iron 0.002mg, Sodium 0.4mg, Vitamin A 0.000275mg and Taurine 0.055mg.

The present invention has carried out electron microscope photograph respectively to the burdock powder obtained in the described preparation method, burdock insoluble dietary fiber powder and burdock nano-sized dietary fiber powder, and the results are shown in Fig. 1-Fig. 3; The specific surface area of burdock nano dietary fiber is increased, and it has a more regular shape and superior physical properties.

Example 3

The present invention prepares the burdock nutritional cereal meal replacement powder with different contents of burdock nano-sized dietary fiber powder and studies the influence on α-glucosidase;

It was found that the structure of α-glucosidase would change from α-helix to β-sheet with the increase of the content of burdock nano-sized dietary fiber powder. The results are shown in Table 1.

Table 1 Effects of different contents of burdock nano-sized dietary fiber powder on the structure of α-glucosidase

It further shows that the burdock nutritional cereal meal replacement powder of the present invention can effectively inhibit the activity of α-glucosidase and lower blood sugar.

Example 4

In the preparation method of the burdock nutritional cereal meal replacement powder of the present invention, the effects of steam explosion conditions and fermentation methods on the physical and chemical properties of the burdock nutritional cereal meal replacement powder are studied. The results are shown in Table 2:

Table 2 Effect of different steam explosion conditions on the physical and chemical properties of insoluble dietary fiber

The results show that: under the steam explosion condition of 1.2MPa/90S, the physicochemical properties of the burdock nutritional cereal meal replacement powder are the most superior.

Example 5

In the preparation method of the burdock nutritional cereal meal replacement powder of the present invention, burdock nanometer insoluble dietary fiber powder (hCNC) with different particle sizes is used to prepare the burdock nutritional cereal meal replacement powder, and the insoluble properties of burdock nanometers with different particle sizes are studied. The kinetic parameters and types of inhibition of dietary fiber powder on α-amylase and α-glucosidase are shown in Table 3 and Table 4:

Table 3 Inhibition kinetic parameters and inhibition types of α-amylase by different particle sizes of hCNC

Table 4 Kinetic parameters and types of inhibition of α-glucosidase by different particle sizes of hCNC

It can be seen from Table 3 and Table 4 that the burdock nano-insoluble dietary fiber powder with different particle sizes has inhibitory effects on both enzymes, and has a stronger inhibitory effect on α-glucosidase, and the smaller the size of hCNCs , the higher the enzyme inhibition efficiency, which may be related to the lower size and easier binding to the active site or other sites of the enzyme.

Simultaneously, the influence of the burdock nano-insoluble dietary fiber powder on the physical and chemical properties of the burdock nutritional cereal meal replacement powder was also analyzed, and the results are shown in Figure 4 and Table 5;

Table 5 Effect of hCNC on viscosity of starch after gelatinization

The results show that: with the increase of the added amount of burdock nano-insoluble dietary fiber powder, the viscosity of the burdock nutritional cereal meal replacement powder is gradually increased, and a stable and orderly structure is formed, and the physical and chemical properties are improved. Slowly digested in the gut, lowering postprandial blood sugar levels.

The present invention has further studied the effect of the burdock nutritional cereal meal replacement powder on the intestinal flora; the results are shown in Figure 5 and Figure 6, and Figure 5 shows the effect of burdock nano dietary fiber powder and burdock grain nutritional powder on the intestinal flora of mice. Effect of relative abundance of bacterial phyla; Figure 6 shows the effect of burdock nano dietary fiber powder and burdock grain nutrition powder on the relative abundance of bacterial genus in the intestinal flora of mice. Among them, CON1 and SPL1 are the comparison group before and after oral administration of burdock nano dietary fiber to mice with normal diet, CON2 and SPL2 are the comparison group before and after administration of high-dose burdock grain meal replacement powder to mice with normal diet, and CON3 and SPL3 are the comparison group before and after oral administration of mice with normal diet Comparison group before and after low-dose burdock cereal meal replacement powder. It can be seen from Figure 5 and Figure 6 that the relative abundance of the two populations of Lachnospiraceae and Lactobacillus in the intestinal flora Firmicutes (Firmicutes) increased significantly. These two intestinal flora can promote the intestinal production of short-chain fatty acids such as butyric acid, etc., which have the effect of lowering blood sugar after being absorbed into the blood. This indicates that the burdock grain nutrition powder has the effect of lowering blood sugar while regulating the diversity of intestinal flora.

validation study

The present invention aims at the hypoglycemic effect of the burdock nutritious cereal meal replacement powder on the type 2 diabetic population, and carried out a living test, specifically as follows:

S1 takes 100 mg of the burdock nutritional cereal meal replacement powder prepared in Example 2,

S2. Prepare reagent samples: get burdock nano-insoluble dietary fiber powder 375mg/ml, burdock grain nutrient powder low dose (burdock powder content) 125mg/ml, burdock grain nutrient powder high dose (burdock powder content) 375mg/ml, wherein solvent is sterile water.

S3. Animal experiment: 75 SD male rats, 160g ~ 180g, were raised under normal rat feeding environment. After 75 rats were fed and maintained with normal feed for 3 days, the animals were fasted for 4 hours, blood was taken from the eye sockets, and blood glucose was detected at this time Value, set as the 0h blood glucose value, after giving 2.5g/kg.bw glucose, measure the 0.5h, 2h blood glucose value, use the 0h, 0.5h, 2h blood glucose value to analyze the glucose tolerance of rats as the grouping standard, and use the glucose tolerance results to divide The rats were divided into 5 groups, 15 rats in each group. The rats in each group continued to maintain the normal diet until one week later, and the model group and the three dosage administration groups were replaced with high-calorie diets. After three weeks of feeding, the model control group After fasting for 24 hours, alloxan 105mg/Kg.bw was intraperitoneally injected with the administration group, and the injection volume was 1ml/100g.bw. After the injection, the high-calorie feed was continued for 3 days, and the experiment was completed on the 33rd day and the rats were sacrificed for follow-up. The experiment, the whole experiment lasted 33 days

In the hypoglycemic experiment, five groups were established, namely the control group (0 blank pair), the model control group (0 model pair), the burdock powder group (7.5B), the low-dose burdock nutritional powder group (2.5A), and the high-dose burdock powder group. Nutrition powder group (7.5A)

Rats in the control group were kept on common diet, during which they were fed with solvent sterile water at 2ml/kg/day for 33 consecutive days. The amount of food given, the amount of sprinkled food, and the amount of leftover food were recorded every week, and the body weight was weighed once.

Rats in the model control group maintained normal feed for 1 week and then switched to high-sugar and high-fat feed. After three weeks of feeding, the model control group was fasted for 24 hours and injected intraperitoneally with alloxan 105mg/Kg.bw, the injection volume was 1ml /100g.bw, continue to give high-calorie feed for 3 days after injection. During the period, they were gavaged with solvent sterile water at 2ml/kg/day for 33 consecutive days. The amount of food given, sprinkled, and leftover food was recorded weekly, and their body weight was weighed once.

Rats in the burdock powder group maintained normal feed for 1 week and then switched to high-sugar and high-fat feed. After three weeks of feeding, the administration group was fasted for 24 hours and injected intraperitoneally with alloxan 105mg/Kg.bw, the injection volume was 1ml /100g.bw, continue to give high-calorie feed for 3 days after injection. During the period, 375mg/ml of burdock powder was administered orally, and the amount of 2ml/kg/day was administered intragastrically, for 33 consecutive days. The subject recorded the amount of food given, the amount of sprinkled food, and the amount of leftover food every week, and weighed once.

Rats in the low-dose burdock nutritional powder group maintained normal feed for 1 week and then switched to high-sugar and high-fat feed for feeding. The amount was 1ml/100g.bw, and the high-calorie feed was continued to be fed for 3 days after the injection. During the period, 125mg/ml of burdock grain nutrition powder was administered orally, and the amount of 2ml/kg/day was administered intragastrically, for 33 consecutive days. The subject recorded the amount of food given, the amount of sprinkled food, and the amount of leftover food every week, and weighed their body weight once.

Rats in the high-dose burdock nutrition powder group maintained normal feed for 1 week and then switched to high-sugar and high-fat feed for feeding. The amount was 1ml/100g.bw, and the high-calorie feed was continued to be fed for 3 days after the injection. During the period, 375mg/ml of burdock grain nutrition powder was administered orally, and the amount of 2ml/kg was administered intragastrically, for 33 days continuously. The subject recorded the amount of food given, the amount of sprinkled food, and the amount of leftover food every week, and weighed their body weight once.

S3. Experimental data analysis: the rats were sacrificed on the 33rd day after the end of the experiment, and the materials were collected, and the animals were fasted for 4 hours to detect fasting blood glucose on the 21st day before the experiment and the 33rd day after the end of the experiment. A semi-automatic biochemical analyzer was used to detect the levels of fasting blood glucose (GLU, mmol/L) and insulin in serum samples. The results of glucose tolerance test and changes of fasting blood glucose and insulin levels in rats in each group are shown in Table 6-Table 10 below.

Table 6 The impact of test samples on fasting blood glucose (mmol/L) in hyperglycemia model rats (XX ± S)

Table 7 The influence of the 0th test sample on the glucose tolerance of hyperglycemia model rats (XX ± S)

Table 8 The influence of the 21d test sample on the glucose tolerance of hyperglycemia model rats (XX ± S)

Table 9 The influence of the 33d test sample on the glucose tolerance of hyperglycemia model rats (XX ± S)

Table 10 The impact of test samples on insulin levels in hyperglycemia model rats (XX ± S)

It can be seen from Table 8 and Table 9 that the fasting blood glucose of the model control was higher than that of the blank control on the 21st and 33rd day. 2.5A, 7.5A group fasting blood sugar and blood sugar drop percentage compared with the model control, the difference is statistically significant (P<0.05), which shows that burdock grain nutrition powder has a significant effect on reducing fasting blood sugar; rat glucose tolerance test data show On the 21st day, the area under the curve of the blood glucose in the 7.5A group was lower than that of the model control group, and the blood glucose values of the 2.5A group and 7.5A group were lower than that of the model control group, and the differences were statistically significant (P<0.05). On the 33rd day, the blood glucose values and the area under the blood glucose curve of the model control group at 0h, 0.5h, and 2h were higher than those of the blank control group, and it was determined that the model glucose metabolism disorder was established. 2.5A group and 7.5A group 0h, 0.5h, 2h blood glucose value and area under the blood glucose curve were lower than the model control group; on the 33rd day 7.5B group 0.5h blood glucose value and blood glucose curve area were lower than the model control group , the differences are statistically significant (P<0.05), which shows that the burdock nutritional cereal meal replacement powder of the present invention has a positive effect on improving the glucose tolerance of rats, and has the effect of lowering blood sugar on rats fed a high-sugar and high-fat diet.

As can be seen from Table 10, the insulin resistance index of the model control group was significantly higher than that of the blank control group, and the insulin resistance index of the 7.5A and 2.5A groups was lower than that of the model control group, and the differences were statistically significant (P<0.05). It shows that the burdock nutrition grain meal replacement powder of the present invention has the effect of reducing insulin resistance to rats fed a high-sugar and high-fat diet.

Further, the present invention has carried out the research on the hypoglycemic effect of the burdock nutritional cereal meal replacement powder on the type 2 diabetic population, specifically as follows:

S1. Get 100 mg/part of the burdock nutritional cereal meal replacement powder prepared in Example 2.

S2. Selection of the subject population: Choose adult patients with type II diabetes mellitus (DM) whose condition is relatively stable after diet control or oral hypoglycemic drug treatment, no need to change the type and dose of drugs, and only take maintenance doses, that is, fasting blood sugar ≥ 7mmol/ L (126mg/dl) or blood glucose ≥11.1mmol/L (200mg/dl) 2 hours after meal; fasting blood glucose 5.6mmol/L-7mmol/L (100mg/dl-126mg/dl) or 2 hours after meal can also be selected Impaired glucose regulation (IGR) population with blood glucose 7.8mmol/L-11.1mmol/L (140mg/dl-200mg/dl).

S3. Experimental grouping: Based on the inclusion and exclusion criteria, volunteers with type 2 diabetes were randomly assigned to the health education group (HEG) and the burdock nutritional cereal meal replacement powder intervention + health education group (HEG+BGG). Among them, the subjects in the health education group (HEG) received the guidance of a professional registered dietitian diet plan; the burdock nutritional cereal meal replacement powder intervention + health education group (HEG+BGG), in addition to health education, this group also gave patients Take 2 bags of burdock grain nutrition powder every day, and take 1 bag of boiled water or milk in the morning and evening. 20g per bag, 40g in total twice a day. Patients are required to take the burdock nutrition grain meal replacement powder first and then eat other foods.

S4. Before the intervention and three months after the intervention, the subjects in the above experimental groups were subjected to physical examination and body composition measurement, including general physical examination and general routine safety indicators, such as blood, urine, and stool routine examination;

Liver and kidney function tests, chest X-ray, electrocardiogram, abdominal B-ultrasound examination, blood uric acid, urine ketones, etc.; detection and observation of human health levels during the experimental period; basic glucose and lipid metabolism indicators were measured during the intervention period of 1 month and 2 months; pre-intervention and During the 3-month intervention period, blood samples were taken from the subjects with an automatic biochemical analyzer to determine the serological indicators of the subjects, including fasting blood glucose, two-hour postprandial blood glucose, and blood lipids (TC, TG, LDL-C, and HDL- C), apo A1, apo B, glycosylated hemoglobin, AST, ALT and bile acid, and insulin were measured using ELISA kits.

S5. Fasting blood glucose test data analysis: the test results are shown in Figure 7.

As can be seen from Figure 7: the postprandial blood glucose level of the health education group (HEG) has no significant change within three months, while the three-month postprandial blood glucose level of the burdock nutritional cereal meal replacement powder intervention group (HEG+GBB) It is obviously in a downward trend, and basically maintained between 6mmol/L and 7mmol/L, blood sugar no longer drops, especially in the second and third months of the intervention group using burdock nutritional cereal meal replacement powder, the blood sugar level is significantly lower than that of the healthy Education group, the difference has statistical significance (P<0.0001), the experimental results show that the burdock nutritional cereal meal replacement powder of the present invention has the effect of reducing postprandial blood sugar and stabilizing postprandial blood sugar in the normal range to hyperglycemia crowd or impaired glucose tolerance crowd. Effect.

Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details and examples shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (9)

1. The burdock nutritious grain meal replacement powder is characterized in that it includes the following ingredients in parts by weight: 20-50 parts by weight of burdock powder, 10-20 parts by weight of soybean powder, 5-15 parts by weight of whey protein powder, 1 part by weight of sesame -10 parts by weight, 3-6 parts by weight of tremella powder, 6-12 parts by weight of wolfberry powder, 9-15 parts by weight of cooked rhizome powder, 5-15 parts by weight of red bean, 1-5 parts by weight of oats, 1-5 parts by weight of quinoa Parts by weight, 6-12 parts by weight of polygonatum powder, 0.001-0.005 parts by weight of iron, 0-1 part by weight of sodium, 0.0002-0.0005 parts by weight of vitamin A and 0.01-0.08 parts by weight of taurine.
2. Burdock nutritious cereal meal replacement powder as claimed in claim 1, is characterized in that, comprises the following components of weight portion: 30 weight portions of burdock powder, 10 weight portions of soybean powder, 10 weight portions of whey protein powder, 5 weight portions of sesame , 5 parts by weight of tremella powder, 10 parts by weight of wolfberry powder, 10 parts by weight of cooked Polygonatum powder, 5 parts by weight of red bean, 4 parts by weight of oats, 5 parts by weight of quinoa, 6 parts by weight of Polygonatum odoratum powder, 0.002 parts by weight of iron, sodium 0.4 parts by weight, vitamin A 0.000275 parts by weight, and taurine 0.055 parts by weight.
3. The burdock nutritious grain meal replacement powder according to claim 1, wherein the burdock powder is: burdock root modified by steam explosion cooperative fermentation, and burdock nano dietary fiber powder prepared by nano-processing.
4. The preparation method of the burdock nutritional cereal meal replacement powder as described in any one of claims 1-3, is characterized in that, comprises the following steps:

   Step 1, preparing burdock nano-dietary fiber powder, specifically comprising:

   The cleaned burdock root slices in step 1.1 are pretreated by steam explosion technology under 1.2MPa/90s steam explosion conditions to obtain modified burdock root slices;

   Step 1.2 Fermentation modification: Dry the modified burdock root slices prepared in step 1 and ultrafinely pulverize them through a 200-mesh sieve, add distilled water at a material-to-liquid ratio of 1:10-15 to obtain a homogenate of burdock root powder. 2-6% of the starter is added, fermented at 38°C-45°C for 2-3 days, sterilized and freeze-dried to obtain modified burdock powder;

   Step 1.3 Nano-processing: the modified burdock powder is subjected to step-by-step enzymatic decontamination at room temperature to obtain burdock insoluble dietary fiber, after the burdock insoluble dietary fiber is mixed with ultrapure water, cellulase is added, and the Process under high-pressure micro-jet conditions, centrifuge and freeze-dry to obtain burdock nano-dietary fiber powder;

   Step 2, preparation of plant raw material powder: grind the washed and dried soybeans, sesame, cooked Polygonatum, medlar, quinoa, oats, red bean and Yuzhu pulverizer to 200-325 mesh, and mix them evenly according to the weight ratio , to prepare plant raw material mixed powder.

   Step 3, irradiation sterilization: the burdock nano-dietary fiber powder and the mixed plant raw material powder are irradiated and sterilized, the irradiation source is Co-60, and the irradiation dose is 5-8kGy.

   Step 4, deployment: mix burdock nano-dietary fiber powder, plant raw material mixed powder, whey protein powder, iron, sodium, vitamins and taurine in proportion, and dry until the water content of the product is 8-15%, to obtain burdock nutritional cereal substitute meal powder.
5. The preparation method of burdock nutrition cereal meal replacement powder as claimed in claim 4, characterized in that, in said step 1, said burdock root slices are prepared by the following steps: after cleaning burdock root with chlorine dioxide spray, cut into Slices with a thickness of 6mm are prepared after blanching in boiling water or soaking in 1.0% citric acid protective agent.
6. The preparation method of burdock nutritional cereal meal replacement powder as claimed in claim 4, characterized in that, the starter is prepared by the following steps: selecting Lactobacillus bulgaricus and Streptococcus thermophilus to inoculate and cultivate, when the number of viable bacteria reaches 109- When 1010, the two are mixed in a ratio of 1:1 to make a starter.
7. The preparation method of burdock nutritional cereal meal replacement powder as claimed in claim 4, characterized in that, in said step 1.3, said burdock insoluble dietary fiber is prepared by the following steps: obtaining modified burdock powder according to the ratio of solid to liquid of 1:10 Homogenize, carry out step-by-step enzymatic removal of impurities in the order of α-amylase, glucoamylase and bromelain at room temperature under ultrasonic conditions. Centrifuge, collect the precipitate, wash with warm water at 60°C, then wash with 80% ethanol by volume, centrifuge again, collect the precipitate, and dry at 40-50°C to obtain burdock insoluble dietary fiber.
8. The preparation method of burdock nutrition cereal meal replacement powder as claimed in claim 4, is characterized in that, in described step 1.3, the ratio of described burdock insoluble dietary fiber and ultrapure water is 1:10-20; The cellulose The amount of enzyme added is 10-20u/g cellulase per gram of burdock insoluble dietary fiber.
9. The preparation method of burdock nutritional cereal meal replacement powder as claimed in claim 4, characterized in that, in the step 1.2, the burdock powder homogenate needs to be sterilized at 121°C for 20 minutes to obtain sterile burdock powder homogenate.

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