WO2023078238A1 - Inhibiteurs de sras-cov-2 pour le traitement d'infections à coronavirus - Google Patents

Inhibiteurs de sras-cov-2 pour le traitement d'infections à coronavirus Download PDF

Info

Publication number
WO2023078238A1
WO2023078238A1 PCT/CN2022/128937 CN2022128937W WO2023078238A1 WO 2023078238 A1 WO2023078238 A1 WO 2023078238A1 CN 2022128937 W CN2022128937 W CN 2022128937W WO 2023078238 A1 WO2023078238 A1 WO 2023078238A1
Authority
WO
WIPO (PCT)
Prior art keywords
alkyl
compound
pharmaceutically acceptable
stereoisomer
acceptable salt
Prior art date
Application number
PCT/CN2022/128937
Other languages
English (en)
Inventor
Xiao DING
Jingjing PENG
Feng Ren
Xiaoyu Ding
Bogdan ZAGRIBELNYY
Yan A. IVANENKOV
Original Assignee
Insilico Medicine Ip Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Insilico Medicine Ip Limited filed Critical Insilico Medicine Ip Limited
Publication of WO2023078238A1 publication Critical patent/WO2023078238A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/26—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • SARS-CoV-2 (also known as 2019-nCoV or COVID-19) first appeared in 2019. Symptoms linked with the disease include fever, myalgia, cough, dyspnea, and fatigue (Huang et al., 2020) . Currently, there is no treatment available for SARS-CoV-2. Nevertheless, treatments with well-known drugs such as chloroquine or investigational drugs such as remdesivir are suggested for this disease (Colson et al., 2020; Wang et al., 2020) .
  • HAV human immunodeficiency virus
  • lopinavir/ritonavir is also being investigated as a therapy for SARS-CoV-2 as they exhibited anti-coronavirus effect in vitro (Que et al., 2003; Chu et al., 2004; Chan et al., 2015; Li and De Clercq, 2020) .
  • SARS-CoV-2 is a beta-coronavirus and is member of the family Coronaviridae, which comprises the largest positive-sense, single-stranded RNA viruses. (Cui et al., 2019) .
  • the virus contains four non-structural proteins: papain-like (PL pro ) and 3-chymotrypsin-like (3CL pro ) proteases, RNA polymerase and helicase (Zumla et al., 2016) . Both proteases (PL pro and 3CL pro ) are involved with transcription and replication of the virus. Amongst the four types, the 3CL pro is considered to be mainly involved in the replication of the virus (de Wit et al., 2016) .
  • 3CLpro hydrolyses the viral polyproteins pp1a and pp1ab to produce functional proteins during coronavirus replication.
  • R 1 is halogen
  • R 2 is halogen
  • Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 1 is - (CR 4 R 4 ) p1 -;
  • each R 4 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 4 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 4a ;
  • p1 is 0-4;
  • R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring B is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 2 is - (CR 9 R 9 ) p2 -;
  • each R 9 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 9 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 9a ;
  • p2 is 0-4;
  • R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring C is cycloalkyl or heterocycloalkyl
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • R c and R d are taken together with the atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R;
  • composition comprising a therapeutically effective amount of a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, and a pharmaceutically acceptable excipient.
  • Also disclosed herein is a method of treating or preventing a coronavirus infection in a patient in need thereof, comprising administering to the patient a compound disclosed herein or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, or the pharmaceutical composition disclosed herein.
  • Also disclosed herein is a method of treating or preventing a SARS-CoV-2 infection in a patient in need thereof, comprising administering to the patient a compound disclosed herein or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, or the pharmaceutical composition disclosed herein.
  • the compound or the pharmaceutical composition is administered to the patient until the infection is reduced or eliminated.
  • the method comprises treating one or more symptoms of SARS-CoV-2 in the patient in need thereof.
  • Also disclosed herein is an in vivo method of inhibiting a protease of SARS-CoV-2, comprising contacting the protease with a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof.
  • the compound binds to a cysteine residue of the protease. In some embodiments, the compound binds reversibly or irreversibly to the cysteine residue. In some embodiments, the compound binds irreversibly to the cysteine residue. In some embodiments, the compound covalently binds to the cysteine residue.
  • the protease is 3CL-protease. In some embodiments, the cysteine is cysteine 145 of 3CL-protease. In some embodiments, the protease is SARS-CoV-2 MPRO.
  • a modified SARS-CoV-2 MPRO protein comprising a SARS-CoV-2 MPRO protein and a compound disclosed herein covalently binds to the SARS-CoV-2 MPRO protein.
  • Carboxyl refers to -COOH.
  • Cyano refers to -CN.
  • Alkyl refers to a straight-chain, or branched-chain saturated hydrocarbon monoradical having from one to about ten carbon atoms, more preferably one to six carbon atoms. Examples include, but are not limited to methyl, ethyl, n-propyl, isopropyl, 2-methyl-1-propyl, 2-methyl-2-propyl, 2-methyl-1-butyl, 3-methyl-1-butyl, 2-methyl-3-butyl, 2, 2-dimethyl-1-propyl, 2-methyl-1-pentyl, 3-methyl-1-pentyl, 4-methyl-1-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 2, 2-dimethyl-1-butyl, 3, 3-dimethyl-1-butyl, 2-ethyl-1-butyl, n-butyl, isobutyl, sec-butyl, t-butyl, n-pentyl, isopent
  • a numerical range such as “C 1 -C 6 alkyl” or “C 1-6 alkyl” means that the alkyl group may consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term “alkyl” where no numerical range is designated.
  • the alkyl is a C 1-10 alkyl.
  • the alkyl is a C 1-6 alkyl.
  • the alkyl is a C 1-5 alkyl.
  • the alkyl is a C 1-4 alkyl.
  • the alkyl is a C 1-3 alkyl.
  • an alkyl group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the alkyl is optionally substituted with oxo, halogen, -CN, -COOH, -COOMe, -OH, -OMe, -NH 2 , or -NO 2 .
  • the alkyl is optionally substituted with halogen, -CN, -OH, or -OMe.
  • the alkyl is optionally substituted with halogen.
  • Alkenyl refers to a straight-chain, or branched-chain hydrocarbon monoradical having one or more carbon-carbon double-bonds and having from two to about ten carbon atoms, more preferably two to about six carbon atoms.
  • a numerical range such as “C 2 -C 6 alkenyl” or “C 2-6 alkenyl” means that the alkenyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term “alkenyl” where no numerical range is designated.
  • an alkenyl group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the alkenyl is optionally substituted with oxo, halogen, -CN, -COOH, -COOMe, -OH, -OMe, -NH 2 , or -NO 2 .
  • the alkenyl is optionally substituted with halogen, -CN, -OH, or -OMe.
  • the alkenyl is optionally substituted with halogen.
  • Alkynyl refers to a straight-chain or branched-chain hydrocarbon monoradical having one or more carbon-carbon triple-bonds and having from two to about ten carbon atoms, more preferably from two to about six carbon atoms. Examples include, but are not limited to ethynyl, 2-propynyl, 2-butynyl, 1, 3-butadiynyl and the like.
  • a numerical range such as “C 2 -C 6 alkynyl” or “C 2-6 alkynyl” means that the alkynyl group may consist of 2 carbon atoms, 3 carbon atoms, 4 carbon atoms, 5 carbon atoms or 6 carbon atoms, although the present definition also covers the occurrence of the term “alkynyl” where no numerical range is designated.
  • an alkynyl group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the alkynyl is optionally substituted with oxo, halogen, -CN, -COOH, COOMe, -OH, -OMe, -NH 2 , or -NO 2 .
  • the alkynyl is optionally substituted with halogen, -CN, -OH, or -OMe.
  • the alkynyl is optionally substituted with halogen.
  • Alkylene refers to a straight or branched divalent hydrocarbon chain. Unless stated otherwise specifically in the specification, an alkylene group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, the alkylene is optionally substituted with oxo, halogen, -CN, -COOH, COOMe, -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, the alkylene is optionally substituted with halogen, -CN, -OH, or -OMe. In some embodiments, the alkylene is optionally substituted with halogen.
  • Alkoxy refers to a radical of the formula -OR a where R a is an alkyl radical as defined. Unless stated otherwise specifically in the specification, an alkoxy group may be optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like. In some embodiments, the alkoxy is optionally substituted with halogen, -CN, -COOH, COOMe, -OH, -OMe, -NH 2 , or -NO 2 . In some embodiments, the alkoxy is optionally substituted with halogen, -CN, -OH, or -OMe. In some embodiments, the alkoxy is optionally substituted with halogen.
  • Aryl refers to a radical derived from a hydrocarbon ring system comprising 6 to 30 carbon atoms and at least one aromatic ring.
  • the aryl radical may be a monocyclic, bicyclic, tricyclic, or tetracyclic ring system, which may include fused (when fused with a cycloalkyl or heterocycloalkyl ring, the aryl is bonded through an aromatic ring atom) or bridged ring systems.
  • the aryl is a 6-to 10-membered aryl.
  • the aryl is a 6-membered aryl (phenyl) .
  • Aryl radicals include, but are not limited to, aryl radicals derived from the hydrocarbon ring systems of anthrylene, naphthylene, phenanthrylene, anthracene, azulene, benzene, chrysene, fluoranthene, fluorene, as-indacene, s-indacene, indane, indene, naphthalene, phenalene, phenanthrene, pleiadene, pyrene, and triphenylene.
  • an aryl may be optionally substituted, for example, with halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the aryl is optionally substituted with halogen, methyl, ethyl, -CN, -COOH, COOMe, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 .
  • the aryl is optionally substituted with halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the aryl is optionally substituted with halogen.
  • Cycloalkyl refers to a partially or fully saturated, monocyclic, or polycyclic carbocyclic ring, which may include fused (when fused with an aryl or a heteroaryl ring, the cycloalkyl is bonded through a non-aromatic ring atom) , spiro, or bridged ring systems. In some embodiments, the cycloalkyl is fully saturated.
  • Representative cycloalkyls include, but are not limited to, cycloalkyls having from three to fifteen carbon atoms (C 3 -C 15 fully saturated cycloalkyl or C 3 -C 15 cycloalkenyl) , from three to ten carbon atoms (C 3 -C 10 fully saturated cycloalkyl or C 3 -C 10 cycloalkenyl) , from three to eight carbon atoms (C 3 -C 8 fully saturated cycloalkyl or C 3 -C 8 cycloalkenyl) , from three to six carbon atoms (C 3 -C 6 fully saturated cycloalkyl or C 3 -C 6 cycloalkenyl) , from three to five carbon atoms (C 3 -C 5 fully saturated cycloalkyl or C 3 -C 5 cycloalkenyl) , or three to four carbon atoms (C 3 -C 4 fully saturated cycloalkyl or C 3 -C
  • the cycloalkyl is a 3-to 10-membered fully saturated cycloalkyl or a 3-to 10-membered cycloalkenyl. In some embodiments, the cycloalkyl is a 3-to 6-membered fully saturated cycloalkyl or a 3-to 6-membered cycloalkenyl. In some embodiments, the cycloalkyl is a 5-to 6-membered fully saturated cycloalkyl or a 5-to 6-membered cycloalkenyl.
  • Monocyclic cycloalkyls include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
  • Polycyclic cycloalkyls include, for example, adamantyl, norbornyl, decalinyl, bicyclo [3.3.0] octane, bicyclo [4.3.0] nonane, cis-decalin, trans-decalin, bicyclo [2.1.1] hexane, bicyclo [2.2.1] heptane, bicyclo [2.2.2] octane, bicyclo [3.2.2] nonane, and bicyclo [3.3.2] decane, and 7, 7-dimethyl-bicyclo [2.2.1] heptanyl.
  • Partially saturated cycloalkyls include, for example cyclopentenyl, cyclohexenyl, cycloheptenyl, and cyclooctenyl.
  • a cycloalkyl is optionally substituted, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • a cycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -COOH, COOMe, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 .
  • a cycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe.
  • the cycloalkyl is optionally substituted with halogen.
  • Halo or “halogen” refers to bromo, chloro, fluoro or iodo. In some embodiments, halogen is fluoro or chloro. In some embodiments, halogen is fluoro.
  • Haloalkyl refers to an alkyl radical, as defined above, that is substituted by one or more halo radicals, as defined above, e.g., trifluoromethyl, difluoromethyl, fluoromethyl, trichloromethyl, 2, 2, 2-trifluoroethyl, 1, 2-difluoroethyl, 3-bromo-2-fluoropropyl, 1, 2-dibromoethyl, and the like.
  • “Hydroxyalkyl” refers to an alkyl radical, as defined above, that is substituted by one or more hydroxyls. In some embodiments, the alkyl is substituted with one hydroxyl. In some embodiments, the alkyl is substituted with one, two, or three hydroxyls. Hydroxyalkyl include, for example, hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl, or hydroxypentyl. In some embodiments, the hydroxyalkyl is hydroxymethyl.
  • Aminoalkyl refers to an alkyl radical, as defined above, that is substituted by one or more amines. In some embodiments, the alkyl is substituted with one amine. In some embodiments, the alkyl is substituted with one, two, or three amines. Aminoalkyl include, for example, aminomethyl, aminoethyl, aminopropyl, aminobutyl, or aminopentyl. In some embodiments, the aminoalkyl is aminomethyl.
  • Heteroalkyl refers to an alkyl group in which one or more skeletal atoms of the alkyl are selected from an atom other than carbon, e.g., oxygen, nitrogen (e.g., -NH-, -N (alkyl) -) , sulfur, phosphorus, or combinations thereof.
  • a heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl.
  • a heteroalkyl is a C 1 -C 6 heteroalkyl wherein the heteroalkyl is comprised of 1 to 6 carbon atoms and one or more atoms other than carbon, e.g., oxygen, nitrogen (e.g.
  • heteroalkyl is attached to the rest of the molecule at a carbon atom of the heteroalkyl.
  • heteroalkyl are, for example, -CH 2 OCH 3 , -CH 2 CH 2 OCH 3 , -CH 2 CH 2 OCH 2 CH 2 OCH 3 , -CH (CH 3 ) OCH 3 , -CH 2 NHCH 3 , -CH 2 N (CH 3 ) 2 , -CH 2 CH 2 NHCH 3 , or -CH 2 CH 2 N (CH 3 ) 2 .
  • a heteroalkyl is optionally substituted for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 .
  • a heteroalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heteroalkyl is optionally substituted with halogen.
  • Heterocycloalkyl refers to a 3-to 24-membered partially or fully saturated ring radical comprising 2 to 23 carbon atoms and from one to 8 heteroatoms selected from the group consisting of nitrogen, oxygen, phosphorous, silicon, and sulfur. In some embodiments, the heterocycloalkyl is fully saturated. In some embodiments, the heterocycloalkyl comprises one to three heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur. In some embodiments, the heterocycloalkyl comprises one to three heteroatoms selected from the group consisting of nitrogen and oxygen. In some embodiments, the heterocycloalkyl comprises one to three nitrogens. In some embodiments, the heterocycloalkyl comprises one or two nitrogens.
  • the heterocycloalkyl comprises one nitrogen. In some embodiments, the heterocycloalkyl comprises one nitrogen and one oxygen.
  • the heterocycloalkyl radical may be a monocyclic, bicyclic, tricyclic, or tetracyclic ring system, which may include fused (when fused with an aryl or a heteroaryl ring, the heterocycloalkyl is bonded through a non-aromatic ring atom) , spiro, or bridged ring systems; and the nitrogen, carbon, or sulfur atoms in the heterocycloalkyl radical may be optionally oxidized; the nitrogen atom may be optionally quaternized.
  • heterocycloalkyls include, but are not limited to, heterocycloalkyls having from two to fifteen carbon atoms (C 2 -C 15 fully saturated heterocycloalkyl or C 2 -C 15 heterocycloalkenyl) , from two to ten carbon atoms (C 2 -C 10 fully saturated heterocycloalkyl or C 2 -C 10 heterocycloalkenyl) , from two to eight carbon atoms (C 2 -C 8 fully saturated heterocycloalkyl or C 2 -C 8 heterocycloalkenyl) , from two to seven carbon atoms (C 2 -C 7 fully saturated heterocycloalkyl or C 2 -C 7 heterocycloalkenyl) , from two to six carbon atoms (C 2 -C 6 fully saturated heterocycloalkyl or C 2 -C 6 heterocycloalkenyl) , from two to five carbon atoms (C 2 -C 5 fully saturated heterocycloalkyl or C 2 -C 5
  • heterocycloalkyl radicals include, but are not limited to, aziridinyl, azetidinyl, oxetanyl, dioxolanyl, thienyl [1, 3] dithianyl, decahydroisoquinolyl, imidazolinyl, imidazolidinyl, isothiazolidinyl, isoxazolidinyl, morpholinyl, octahydroindolyl, octahydroisoindolyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolidinyl, oxazolidinyl, piperidinyl, piperazinyl, 4-piperidonyl, pyrrolidinyl, pyrazolidinyl, quinuclidinyl, thiazolidinyl, tetrahydrofuryl, trithianyl, tetrahydropyranyl
  • heterocycloalkyl also includes all ring forms of the carbohydrates, including but not limited to the monosaccharides, the disaccharides, and the oligosaccharides.
  • heterocycloalkyls have from 2 to 10 carbons in the ring. It is understood that when referring to the number of carbon atoms in a heterocycloalkyl, the number of carbon atoms in the heterocycloalkyl is not the same as the total number of atoms (including the heteroatoms) that make up the heterocycloalkyl (i.e. skeletal atoms of the heterocycloalkyl ring) .
  • the heterocycloalkyl is a 3-to 8-membered heterocycloalkyl.
  • the heterocycloalkyl is a 3-to 7-membered heterocycloalkyl. In some embodiments, the heterocycloalkyl is a 3-to 6-membered heterocycloalkyl. In some embodiments, the heterocycloalkyl is a 4-to 6-membered heterocycloalkyl. In some embodiments, the heterocycloalkyl is a 5-to 6-membered heterocycloalkyl. In some embodiments, the heterocycloalkyl is a 3-to 8-membered heterocycloalkenyl. In some embodiments, the heterocycloalkyl is a 3-to 7-membered heterocycloalkenyl.
  • the heterocycloalkyl is a 3-to 6-membered heterocycloalkenyl. In some embodiments, the heterocycloalkyl is a 4-to 6-membered heterocycloalkenyl. In some embodiments, the heterocycloalkyl is a 5-to 6-membered heterocycloalkenyl.
  • a heterocycloalkyl may be optionally substituted as described below, for example, with oxo, halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the heterocycloalkyl is optionally substituted with oxo, halogen, methyl, ethyl, -CN, -COOH, COOMe, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 .
  • the heterocycloalkyl is optionally substituted with halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heterocycloalkyl is optionally substituted with halogen.
  • Heteroaryl refers to a 5-to 14-membered ring system radical comprising one to thirteen carbon atoms, one to six heteroatoms selected from the group consisting of nitrogen, oxygen, phosphorous, and sulfur, and at least one aromatic ring.
  • the heteroaryl comprises one to three heteroatoms selected from the group consisting of nitrogen, oxygen, and sulfur.
  • the heteroaryl comprises one to three heteroatoms selected from the group consisting of nitrogen and oxygen.
  • the heteroaryl comprises one to three nitrogens.
  • the heteroaryl comprises one or two nitrogens.
  • the heteroaryl comprises one nitrogen.
  • the heteroaryl radical may be a monocyclic, bicyclic, tricyclic, or tetracyclic ring system, which may include fused (when fused with a cycloalkyl or heterocycloalkyl ring, the heteroaryl is bonded through an aromatic ring atom) or bridged ring systems; and the nitrogen, carbon or sulfur atoms in the heteroaryl radical may be optionally oxidized; the nitrogen atom may be optionally quaternized.
  • the heteroaryl is a 5-to 10-membered heteroaryl.
  • the heteroaryl is a 5-to 6-membered heteroaryl.
  • the heteroaryl is a 6-membered heteroaryl.
  • the heteroaryl is a 5-membered heteroaryl.
  • examples include, but are not limited to, azepinyl, acridinyl, benzimidazolyl, benzothiazolyl, benzindolyl, benzodioxolyl, benzofuranyl, benzooxazolyl, benzothiazolyl, benzothiadiazolyl, benzo [b] [1, 4] dioxepinyl, 1, 4-benzodioxanyl, benzonaphthofuranyl, benzoxazolyl, benzodioxolyl, benzodioxinyl, benzopyranyl, benzopyranonyl, benzofuranyl, benzofuranonyl, benzothienyl (benzothiophenyl) , benzotriazolyl, benzo [4, 6] imidazo [1, 2-a] pyridinyl, carbazolyl, cinnolinyl,
  • a heteroaryl may be optionally substituted, for example, with halogen, amino, nitrile, nitro, hydroxyl, alkyl, alkenyl, alkynyl, haloalkyl, alkoxy, carboxyl, carboxylate, aryl, cycloalkyl, heterocycloalkyl, heteroaryl, and the like.
  • the heteroaryl is optionally substituted with halogen, methyl, ethyl, -CN, -COOH, COOMe, -CF 3 , -OH, -OMe, -NH 2 , or -NO 2 .
  • the heteroaryl is optionally substituted with halogen, methyl, ethyl, -CN, -CF 3 , -OH, or -OMe. In some embodiments, the heteroaryl is optionally substituted with halogen.
  • an optionally substituted group may be un-substituted (e.g., -CH 2 CH 3 ) , fully substituted (e.g., -CF 2 CF 3 ) , mono-substituted (e.g., -CH 2 CH 2 F) or substituted at a level anywhere in-between fully substituted and mono-substituted (e.g., -CH 2 CHF 2 , -CH 2 CF 3 , -CF 2 CH 3 , -CFHCHF 2 , etc. ) .
  • any substituents described should generally be understood as having a maximum molecular weight of about 1,000 daltons, and more typically, up to about 500 daltons.
  • an “effective amount” or “therapeutically effective amount” refers to an amount of a compound administered to a mammalian subject, either as a single dose or as part of a series of doses, which is effective to produce a desired therapeutic effect.
  • Treatment of an individual (e.g. a mammal, such as a human) or a cell is any type of intervention used in an attempt to alter the natural course of the individual or cell.
  • treatment includes administration of a pharmaceutical composition, subsequent to the initiation of a pathologic event or contact with an etiologic agent and includes stabilization of the condition (e.g., condition does not worsen) or alleviation of the condition.
  • the present disclosure includes compounds and/or materials for use as SARS-CoV-2 inhibitors and for treating a subject infected with SARS-CoV-2.
  • R 1 is halogen
  • R 2 is halogen
  • Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 1 is - (CR 4 R 4 ) p1 -;
  • each R 4 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 4 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 4a ;
  • p1 is 0-4;
  • R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring B is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 2 is - (CR 9 R 9 ) p2 -;
  • each R 9 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 9 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 9a ;
  • p2 is 0-4;
  • R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring C is cycloalkyl or heterocycloalkyl
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • R c and R d are taken together with the atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R;
  • R 1 is halogen
  • R 2 is halogen
  • Ring A is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 1 is - (CR 4 R 4 ) p1 -;
  • each R 4 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 4 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 4a ;
  • p1 is 0-4;
  • R 5 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring B is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl;
  • n 0-4;
  • L 2 is - (CR 9 R 9 ) p2 -;
  • each R 9 is independently hydrogen, halogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, or C 1 -C 6 heteroalkyl;
  • R 9 on the same carbon are taken together to form a cycloalkyl or heterocycloalkyl, each optionally substituted with one or more R 9a ;
  • p2 is 0-4;
  • R 7 is hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl;
  • Ring C is cyclohexyl
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkyl (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R;
  • R c and R d are taken together with the atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R;
  • R 1 is fluoro or chloro. In some embodiments of a compound of Formula (II) , R 2 is fluoro or chloro. In some embodiments of a compound of Formula (II) , R 1 is fluoro and R 2 is chloro. In some embodiments of a compound of Formula (II) , R 1 is chloro and R 2 is fluoro.
  • Ring A is aryl or heteroaryl. In some embodiments of a compound of Formula (II) , Ring A is phenyl. In some embodiments of a compound of Formula (II) , Ring A is 6-membered heteroaryl. In some embodiments of a compound of Formula (II) , Ring A is monocyclic ring. In some embodiments of a compound of Formula (II) , Ring A is bicyclic ring. In some embodiments of a compound of Formula (II) , Ring A is tricyclic ring.
  • R 3 is not CN. In some embodiments of a compound of Formula (II) , R 3 is not CN when Ring A is phenyl.
  • each R 3 is independently -OR a or -SR a .
  • each R 3 is independently -OR a .
  • each R 3a is independently halogen, -CN, -OH, -OR a , -NR c R d , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 3a is independently halogen, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • n is 1 or 2. In some embodiments of a compound of Formula (II) , n is 1-3. In some embodiments of a compound of Formula (II) , n is 0-2. In some embodiments of a compound of Formula (II) , n is 1. In some embodiments of a compound of Formula (II) , n is 2. In some embodiments of a compound of Formula (II) , n is 3. In some embodiments of a compound of Formula (II) , n is 4.
  • p1 is 0. In some embodiments of a compound of Formula (II) , p1 is 1. In some embodiments of a compound of Formula (II) , p1 is 2. In some embodiments of a compound of Formula (II) , p1 is 3. In some embodiments of a compound of Formula (II) , p1 is 1-3. In some embodiments of a compound of Formula (II) , p1 is 0-2. In some embodiments of a compound of Formula (II) , p1 is 0 or 1. In some embodiments of a compound of Formula (II) , p1 is 1 or 2.
  • each R 4 is independently hydrogen, halogen, or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , each R 4 is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , each R 4 is independently hydrogen or halogen. In some embodiments of a compound of Formula (II) , each R 4 is hydrogen.
  • each R 4a is independently halogen, -CN, -OH, -OR a , -NR c R d , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 4a is independently halogen, -OH, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • each R 5 is hydrogen, deuterium or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , each R 5 is hydrogen. In some embodiments of a compound of Formula (II) , each R 5 is deuterium or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , each R 5 is deuterium, C 1 -C 6 alkyl, or C 1 -C 6 deuteroalkyl. In some embodiments of a compound of Formula (II) , each R 5 is C 1 -C 6 alkyl.
  • each R 5 is methyl. In some embodiments of a compound of Formula (II) , each R 5 is CD 3 . In some embodiments of a compound of Formula (II) , each R 5 is deuterium.
  • Ring B is heterocycloalkyl or heteroaryl. In some embodiments of a compound of Formula (II) , Ring B is heteroaryl. In some embodiments of a compound of Formula (II) , Ring B is 5-or 6-membered heteroaryl. In some embodiments of a compound of Formula (II) , Ring B is 6-membered heteroaryl.
  • each R 6 is independently halogen, -CN, -OH, -OR a , -NR c R d , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 6 is independently halogen or C 1 -C 6 alkyl.
  • each R 6a is independently halogen, -CN, -OH, -OR a , -NR c R d , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • m is 0-3. In some embodiments of a compound of Formula (II) , m is 1-3. In some embodiments of a compound of Formula (II) , m is 0-2. In some embodiments of a compound of Formula (II) , m is 0 or 1. In some embodiments of a compound of Formula (II) , m is 1 or 2. In some embodiments of a compound of Formula (II) , m is 0. In some embodiments of a compound of Formula (II) , m is 1. In some embodiments of a compound of Formula (II) , m is 2. In some embodiments of a compound of Formula (II) , m is 3.
  • R 7 is hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , R 7 is C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , R 7 is hydrogen.
  • p2 is 0. In some embodiments of a compound of Formula (II) , p2 is 1. In some embodiments of a compound of Formula (II) , p2 is 2. In some embodiments of a compound of Formula (II) , p2 is 3. In some embodiments of a compound of Formula (II) , p2 is 1-3. In some embodiments of a compound of Formula (II) , p2 is 0-2. In some embodiments of a compound of Formula (II) , p2 is 0 or 1. In some embodiments of a compound of Formula (II) , p2 is 1 or 2.
  • each R 9 is independently hydrogen, halogen, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 9 is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound of Formula (II) , each R 9 is independently hydrogen or halogen. In some embodiments of a compound of Formula (II) , each R 9 is hydrogen. In some embodiments of a compound of Formula (II) , each R 9 is independently hydrogen, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 9 is independently hydrogen or C 1 -C 6 haloalkyl.
  • each R 9a is independently halogen, -CN, -OH, -OR a , -NR c R d , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl. In some embodiments of a compound of Formula (II) , each R 9a is independently halogen, -OH, C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • Ring C is cycloalkyl. In some embodiments of a compound of Formula (II) , Ring C is cyclohexyl. In some embodiments of a compound of Formula (II) , Ring C is heterocycloalkyl. In some embodiments of a compound of Formula (II) , Ring C is monocyclic heterocycloalkyl.
  • each R 10 is independently halogen, -CN, -OH, -OR a , - NR c R d , C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, or heterocycloalkyl.
  • each R 10 is independently halogen, -OH, -OR a , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • each R 10 is independently halogen.
  • q is 0-2. In some embodiments of a compound of Formula (II) , q is 1-3. In some embodiments of a compound of Formula (II) , q is 1 or 2. In some embodiments of a compound of Formula (II) , q is 0 or 1. In some embodiments of a compound of Formula (II) , q is 0. In some embodiments of a compound of Formula (II) , q is 1. In some embodiments of a compound of Formula (II) , q is 2. In some embodiments of a compound of Formula (II) , q is 3. In some embodiments of a compound of Formula (II) , q is 4.
  • a compound of Formula (II) is In some embodiments of a compound of Formula (II) , is In some embodiments of a compound of Formula (II) , is
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkyl (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkyl (aryl) , or C 1 -C 6 alkyl (heteroaryl) ; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R.
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R.
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkylene (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkylene (aryl) , or C 1 -C 6 alkylene (heteroaryl) .
  • each R a is independently C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl. In some embodiments of a compound disclosed herein, each R a is independently C 1 -C 6 alkyl or C 1 -C 6 haloalkyl. In some embodiments of a compound disclosed herein, each R a is independently C 1 -C 6 alkyl. In some embodiments of a compound disclosed herein, each R a is independently C 1 -C 6 haloalkyl.
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkylene (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkylene (aryl) , or C 1 -C 6 alkylene (heteroaryl) ; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R.
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R.
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkylene (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkylene (aryl) , or C 1 -C 6 alkylene (heteroaryl) .
  • each R b is independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl. In some embodiments of a compound disclosed herein, each R b is independently hydrogen, C 1 -C 6 alkyl or C 1 -C 6 haloalkyl. In some embodiments of a compound disclosed herein, each R b is independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound disclosed herein, each R b is hydrogen. In some embodiments of a compound disclosed herein, each R b is independently C 1 -C 6 alkyl.
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkylene (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkylene (aryl) , or C 1 -C 6 alkylene (heteroaryl) ; wherein each alkyl, cycloalkyl, heterocycloalkyl, aryl, and heteroaryl is independently optionally substituted with one or more R.
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl; wherein each alkyl, cycloalkyl, and heterocycloalkyl is independently optionally substituted with one or more R.
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 hydroxyalkyl, C 1 -C 6 aminoalkyl, C 1 -C 6 heteroalkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, C 1 -C 6 alkylene (cycloalkyl) , C 1 -C 6 alkylene (heterocycloalkyl) , C 1 -C 6 alkylene (aryl) , or C 1 -C 6 alkyl (heteroaryl) .
  • each R c and R d are independently hydrogen, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, or cycloalkyl, heterocycloalkyl. In some embodiments of a compound disclosed herein, each R c and R d are independently hydrogen, C 1 -C 6 alkyl or C 1 -C 6 haloalkyl. In some embodiments of a compound disclosed herein, each R c and R d are independently hydrogen or C 1 -C 6 alkyl. In some embodiments of a compound disclosed herein, each R c and R d are hydrogen. In some embodiments of a compound disclosed herein, each R c and R d are independently C 1 -C 6 alkyl.
  • R c and R d are taken together with the atom to which they are attached to form a heterocycloalkyl optionally substituted with one or more R.
  • each R is independently halogen, -CN, -OH, -OC 1 -C 6 alkyl, -NH 2 , C 1 -C 6 alkyl, or C 1 -C 6 haloalkyl.
  • each R 3 , R 6 , R 8 , R a , R b , R c , R d , the ring formed when: two R 4 are taken together, R 7 and R 8 are taken together, and R c and R d are taken together, is optionally and independently substituted with one, two, three, or four substituents as defined herein.
  • each R 3 , R 6 , R 8 , R a , R b , R c , R d , the ring formed when: two R 4 are taken together, R 7 and R 8 are taken together, and R c and R d are taken together, is optionally and independently substituted with one, two, or three substituents as defined herein.
  • each R 3 , R 6 , R 8 , R a , R b , R c , R d , the ring formed when: two R 4 are taken together, R 7 and R 8 are taken together, and R c and R d are taken together, is optionally and independently substituted with one or two substituents as defined herein.
  • one or more hydrogen on Ring A, Ring B, or Ring C is replaced with one or more deuteriums.
  • one or more of R, R 3 , R 3a , R 4 , R 4a , R 5 , R 6 , R 6a , R 7 , R 7a , R 8 , R 8a , R 9 , R 9a , R 10 , R a , R b , R c , and/or R d groups comprise deuterium at a percentage higher than the natural abundance of deuterium.
  • one or more hydrogens are replaced with one or more deuteriums in one or more of the following groups R, R 3 , R 3a , R 4 , R 4a , R 5 , R 6 , R 6a , R 7 , R 7a , R 8 , R 8a , R 9 , R 9a , R 10 , R a , R b , R c , and/or R d .
  • the abundance of deuterium in each of R, R 3 , R 3a , R 4 , R 4a , R 5 , R 6 , R 6a , R 7 , R 7a , R 8 , R 8a , R 9 , R 9a , R 10 , R a , R b , R c , and/or R d is independently at least 1%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or 100%of a total number of hydrogen and deuterium.
  • the compound disclosed herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof is one of the compounds in Table 1.
  • the compounds described herein exist as geometric isomers. In some embodiments, the compounds described herein possess one or more double bonds. The compounds presented herein include all cis, trans, syn, anti,
  • Z) isomers as well as the corresponding mixtures thereof. In some situations, the compounds described herein possess one or more chiral centers and each center exists in the R configuration, or S configuration. The compounds described herein include all diastereomeric, enantiomeric, and epimeric forms as well as the corresponding mixtures thereof.
  • mixtures of enantiomers and/or diastereoisomers, resulting from a single preparative step, combination, or interconversion are useful for the applications described herein.
  • the compounds described herein are prepared as their individual stereoisomers by reacting a racemic mixture of the compound with an optically active resolving agent to form a pair of diastereoisomeric compounds, separating the diastereomers and recovering the optically pure enantiomers.
  • dissociable complexes are preferred.
  • the diastereomers have distinct physical properties (e.g., melting points, boiling points, solubilities, reactivity, etc.
  • the diastereomers are separated by chiral chromatography, or preferably, by separation/resolution techniques based upon differences in solubility.
  • the optically pure enantiomer is then recovered, along with the resolving agent, by any practical means that would not result in racemization.
  • the compounds described herein exist in their isotopically-labeled forms.
  • the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds.
  • the methods disclosed herein include methods of treating diseases by administering such isotopically-labeled compounds as pharmaceutical compositions.
  • the compounds disclosed herein include isotopically-labeled compounds, which are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature.
  • isotopes examples include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine, and chloride, such as 2 H (D) , 3 H, 13 C, 14 C, l5 N, 18 O, 17 O, 31 P, 32 P, 35 S, 18 F, and 36 Cl, respectively.
  • Compounds described herein, and the pharmaceutically acceptable salts, solvates, or stereoisomers thereof which contain the aforementioned isotopes and/or other isotopes of other atoms are within the scope of this disclosure.
  • isotopically-labeled compounds for example those into which radioactive isotopes such as 3 H and 14 C are incorporated, are useful in drug and/or substrate tissue distribution assays. Tritiated, i.e., 3 H and carbon-14, i.e., 14 C, isotopes are particularly preferred for their ease of preparation and detectability.
  • the abundance of deuterium in each of the substituents disclosed herein is independently at least 1%, at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or 100%of a total number of hydrogen and deuterium.
  • one or more of the substituents disclosed herein comprise deuterium at a percentage higher than the natural abundance of deuterium.
  • one or more hydrogens are replaced with one or more deuteriums in one or more of the substituents disclosed herein.
  • the compounds described herein are labeled by other means, including, but not limited to, the use of chromophores or fluorescent moieties, bioluminescent labels, or chemiluminescent labels.
  • the compounds described herein exist as their pharmaceutically acceptable salts.
  • the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts.
  • the methods disclosed herein include methods of treating diseases by administering such pharmaceutically acceptable salts as pharmaceutical compositions.
  • the compounds described herein possess acidic or basic groups and therefore react with any of a number of inorganic or organic bases, and inorganic and organic acids, to form a pharmaceutically acceptable salt.
  • these salts are prepared in situ during the final isolation and purification of the compounds disclosed herein, or a solvate, or stereoisomer thereof, or by separately reacting a purified compound in its free form with a suitable acid or base, and isolating the salt thus formed.
  • Examples of pharmaceutically acceptable salts include those salts prepared by reaction of the compounds described herein with a mineral, organic acid or inorganic base, such salts including, acetate, acrylate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, bisulfite, bromide, butyrate, butyn-1, 4-dioate, camphorate, camphorsulfonate, caproate, caprylate, chlorobenzoate, chloride, citrate, cyclopentanepropionate, decanoate, digluconate, dihydrogenphosphate, dinitrobenzoate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptanoate, glycerophosphate, glycolate, hemisulfate, heptanoate, hexanoate, hexyne-1, 6-dioate, hydroxybenzoate,
  • the compounds described herein can be prepared as pharmaceutically acceptable salts formed by reacting the free base form of the compound with a pharmaceutically acceptable inorganic or organic acid, including, but not limited to, inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid metaphosphoric acid, and the like; and organic acids such as acetic acid, propionic acid, hexanoic acid, cyclopentanepropionic acid, glycolic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, p-toluenesulfonic acid, tartaric acid, trifluoroacetic acid, citric acid, benzoic acid, 3- (4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, arylsulfonic acid, methanesulfonic acid, ethanesulfonic acid, 1, 2-ethanedis
  • other acids such as oxalic, while not in themselves pharmaceutically acceptable, are employed in the preparation of salts useful as intermediates in obtaining the compounds disclosed herein, solvate, or stereoisomer thereof and their pharmaceutically acceptable acid addition salts.
  • those compounds described herein which comprise a free acid group react with a suitable base, such as the hydroxide, carbonate, bicarbonate, sulfate, of a pharmaceutically acceptable metal cation, with ammonia, or with a pharmaceutically acceptable organic primary, secondary, tertiary, or quaternary amine.
  • a suitable base such as the hydroxide, carbonate, bicarbonate, sulfate, of a pharmaceutically acceptable metal cation, with ammonia, or with a pharmaceutically acceptable organic primary, secondary, tertiary, or quaternary amine.
  • Representative salts include the alkali or alkaline earth salts, like lithium, sodium, potassium, calcium, and magnesium, and aluminum salts and the like.
  • bases include sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate, N + (C 1-4 alkyl) 4 , and the like.
  • Organic amines useful for the formation of base addition salts include ethylamine, diethylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine and the like. It should be understood that the compounds described herein also include the quaternization of any basic nitrogen-containing groups they contain. In some embodiments, water or oil-soluble or dispersible products are obtained by such quaternization.
  • the compounds described herein exist as solvates.
  • the disclosure provides for methods of treating diseases by administering such solvates.
  • the disclosure further provides for methods of treating diseases by administering such solvates as pharmaceutical compositions.
  • Solvates contain either stoichiometric or non-stoichiometric amounts of a solvent, and, in some embodiments, are formed during the process of crystallization with pharmaceutically acceptable solvents such as water, ethanol, and the like. Hydrates are formed when the solvent is water, or alcoholates are formed when the solvent is alcohol. Solvates of the compounds described herein can be conveniently prepared or formed during the processes described herein. By way of example only, hydrates of the compounds described herein can be conveniently prepared by recrystallization from an aqueous/organic solvent mixture, using organic solvents including, but not limited to, dioxane, tetrahydrofuran or methanol.
  • the compounds provided herein can exist in unsolvated as well as solvated forms. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the compounds and methods provided herein.
  • Tautomers are compounds that are interconvertible by migration of a hydrogen atom, accompanied by a switch of a single bond and adjacent double bond. In bonding arrangements where tautomerization is possible, a chemical equilibrium of the tautomers will exist. All tautomeric forms of the compounds disclosed herein are contemplated. The exact ratio of the tautomers depends on several factors, including temperature, solvent, and pH.
  • the coronavirus infection is caused by the SARS-CoV-2 virus.
  • the coronavirus infection is caused by the MERS-CoV virus.
  • the coronavirus infection is caused by the SARS-CoV virus.
  • the coronavirus infection is caused by the HCoV-229E virus.
  • the coronavirus infection is caused by the HCoV-OC43 virus.
  • the coronavirus infection is caused by the HCoV-NL63 virus.
  • the coronavirus infection is caused by the HCoV-HKU1 virus.
  • a method of treating or preventing a SARS-CoV-2 infection in a patient in need thereof comprising administering to the patient a compound or a pharmaceutical composition comprising a compound described herein, for example, a compound of Formula (II) .
  • the compound disclosed herein is administered to the subject prophylactically.
  • the subject is suspected of having a SARS-CoV-2 infection before the SARS-CoV-2 infection is diagnosed.
  • the compounds of the present disclosure are administered to the subject until the infection is treated, inhibited, or reduced. In some embodiments, the compounds is administered to the subject until one or more symptoms of the SARS-CoV-2 infection is reduced.
  • a method of inhibiting a viral infection comprising providing a compound disclosed herein to the infection so as to inhibit the viral infection.
  • the viral infection is caused by SARS-CoV-2.
  • the viral infection is caused by MERS-CoV.
  • the viral infection is caused by SARS-CoV.
  • the viral infection is caused by HCoV-229E.
  • the viral infection is caused by HCoV-OC43.
  • the viral infection is caused by HCoV-NL63.
  • the viral infection is caused by HCoV-HKU1.
  • a method of inhibiting SARS-CoV-2 by binding with a protein thereof comprising providing a compound disclosed herein to a SARS-CoV-2 so as to inhibit the SARS-CoV-2.
  • the SARS-CoV-2 binds to a protease on the SARS-CoV-2.
  • the compounds disclosed herein bind with a cysteine residue of the main protease, thereby inhibiting the SARS-CoV-2.
  • the cysteine residue is at position 145 of a main protease.
  • the protease is 3CL.
  • compositions containing the compound (s) described herein are administered for prophylactic and/or therapeutic treatments.
  • the compositions are administered to a patient already suffering from a disease or condition, in an amount sufficient to cure or at least partially arrest at least one of the symptoms of the disease or condition. Amounts effective for this use depend on the severity and course of the disease or condition, previous therapy, the patient’s health status, weight, and response to the drugs, and the judgment of the treating physician.
  • Therapeutically effective amounts are optionally determined by methods including, but not limited to, a dose escalation and/or dose ranging clinical trial.
  • compositions containing the compounds described herein are administered to a patient susceptible to or otherwise at risk of a particular disease, disorder, or condition.
  • a patient susceptible to or otherwise at risk of a particular disease, disorder, or condition is defined to be a “prophylactically effective amount or dose. ”
  • the precise amounts also depend on the patient’s state of health, weight, and the like.
  • effective amounts for this use will depend on the severity and course of the disease, disorder or condition, previous therapy, the patient’s health status and response to the drugs, and the judgment of the treating physician.
  • prophylactic treatments include administering to a mammal, who previously experienced at least one symptom of or risk factor for the disease being treated and is currently in remission, a pharmaceutical composition comprising a compound described herein, or a pharmaceutically acceptable salt thereof, in order to prevent a return of the symptoms of the disease or condition.
  • the administration of the compounds are administered chronically, that is, for an extended period of time, including throughout the duration of the patient’s life in order to ameliorate or otherwise control or limit the symptoms of the patient’s disease or condition.
  • the dose of drug being administered is temporarily reduced or temporarily suspended for a certain length of time (i.e., a “drug holiday” ) .
  • the length of the drug holiday is between 2 days and 1 year, including by way of example only, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, 12 days, 15 days, 20 days, 28 days, or more than 28 days.
  • the dose reduction during a drug holiday is, by way of example only, by 10%-100%, including by way of example only 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, and 100%.
  • a maintenance dose is administered if necessary. Subsequently, in specific embodiments, the dosage, or the frequency of administration, or both, is reduced, as a function of the symptoms, to a level at which the improved disease, disorder or condition is retained. In certain embodiments, however, the patient requires intermittent or daily treatment on a long-term basis upon any recurrence of symptoms.
  • the amount of a given agent that corresponds to such an amount varies depending upon factors such as the particular compound, disease condition and its severity, the identity (e.g., weight, sex) of the subject or host in need of treatment, but nevertheless is determined according to the particular circumstances surrounding the case, including, e.g., the specific agent being administered, the route of administration, the condition being treated, and the subject or host being treated.
  • doses employed for adult human treatment are typically in the range of 0.01 mg-5000 mg per day. In one aspect, doses employed for adult human treatment are from about 1 mg to about 1000 mg per day. In one embodiment, the desired dose is conveniently presented in a single dose or in divided doses administered simultaneously or at appropriate intervals, for example as two, three, four or more sub-doses per day.
  • the daily dosages appropriate for the compound described herein, or a pharmaceutically acceptable salt thereof are from about 0.01 to about 50 mg/kg per body weight. In some embodiments, the daily dosage, or the amount of active in the dosage form are lower or higher than the ranges indicated herein, based on a number of variables in regard to an individual treatment regime. In various embodiments, the daily and unit dosages are altered depending on a number of variables including, but not limited to, the activity of the compound used, the disease or condition to be treated, the mode of administration, the requirements of the individual subject, the severity of the disease or condition being treated, and the judgment of the practitioner.
  • Toxicity and therapeutic efficacy of such therapeutic regimens are determined by standard pharmaceutical procedures in cell cultures or experimental animals, including, but not limited to, the determination of the LD 10 and the ED 90 .
  • the dose ratio between the toxic and therapeutic effects is the therapeutic index and it is expressed as the ratio between LD 50 and ED 50 .
  • the data obtained from cell culture assays and animal studies are used in formulating the therapeutically effective daily dosage range and/or the therapeutically effective unit dosage amount for use in mammals, including humans.
  • the daily dosage amount of the compounds described herein lies within a range of circulating concentrations that include the ED 50 with minimal toxicity.
  • the daily dosage range and/or the unit dosage amount varies within this range depending upon the dosage form employed and the route of administration utilized.
  • the effective amount of the compound described herein, or a pharmaceutically acceptable salt thereof is: (a) systemically administered to the mammal; and/or (b) administered orally to the mammal; and/or (c) intravenously administered to the mammal; and/or (d) administered by injection to the mammal; and/or (e) administered topically to the mammal; and/or (f) administered non-systemically or locally to the mammal.
  • any of the aforementioned aspects are further embodiments comprising single administrations of the effective amount of the compound, including further embodiments in which (i) the compound is administered once a day; or (ii) the compound is administered to the mammal multiple times over the span of one day.
  • any of the aforementioned aspects are further embodiments comprising multiple administrations of the effective amount of the compound, including further embodiments in which (i) the compound is administered continuously or intermittently: as in a single dose; (ii) the time between multiple administrations is every 6 hours; (iii) the compound is administered to the mammal every 8 hours; (iv) the compound is administered to the subject every 12 hours; (v) the compound is administered to the subject every 24 hours.
  • the method comprises a drug holiday, wherein the administration of the compound is temporarily suspended or the dose of the compound being administered is temporarily reduced; at the end of the drug holiday, dosing of the compound is resumed.
  • the length of the drug holiday varies from 2 days to 1 year.
  • Suitable routes of administration include, but are not limited to, oral, intravenous, rectal, aerosol, parenteral, ophthalmic, pulmonary, transmucosal, transdermal, vaginal, otic, nasal, and topical administration.
  • parenteral delivery includes intramuscular, subcutaneous, intravenous, intramedullary injections, as well as intrathecal, direct intraventricular, intraperitoneal, intralymphatic, and intranasal injections.
  • a compound as described herein is administered in a local rather than systemic manner, for example, via injection of the compound directly into an organ, often in a depot preparation or sustained release formulation.
  • long acting formulations are administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.
  • the drug is delivered in a targeted drug delivery system, for example, in a liposome coated with organ specific antibody.
  • the liposomes are targeted to and taken up selectively by the organ.
  • the compound as described herein is provided in the form of a rapid release formulation, in the form of an extended release formulation, or in the form of an intermediate release formulation.
  • the compound described herein is administered topically. In yet other embodiments, the compound described herein is administered by inhalation. In some embodiments, the compounds disclosed herein are formulated for intranasal administration. Such formulations include nasal sprays, nasal mists, and the like.
  • the compounds described herein are administered to a subject in need thereof, either alone or in combination with pharmaceutically acceptable carriers, excipients, or diluents, in a pharmaceutical composition, according to standard pharmaceutical practice.
  • the compounds of this disclosure may be administered to animals.
  • the compounds can be administered orally or parenterally, including the intravenous, intramuscular, intraperitoneal, subcutaneous, rectal, and topical routes of administration.
  • compositions comprising a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, and at least one pharmaceutically acceptable excipient.
  • Pharmaceutical compositions are formulated in a conventional manner using one or more pharmaceutically acceptable excipients that facilitate processing of the active compounds into preparations that can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen.
  • a summary of pharmaceutical compositions described herein can be found, for example, in Remington: The Science and Practice of Pharmacy, Nineteenth Ed (Easton, Pa.: Mack Publishing Company, 1995) ; Hoover, John E., Remington’s Pharmaceutical Sciences, Mack Publishing Co., Easton, Pennsylvania 1975; Liberman, H.A.
  • the pharmaceutically acceptable excipient is selected from carriers, binders, filling agents, suspending agents, flavoring agents, sweetening agents, disintegrating agents, dispersing agents, surfactants, lubricants, colorants, diluents, solubilizers, moistening agents, plasticizers, stabilizers, penetration enhancers, wetting agents, anti-foaming agents, antioxidants, preservatives, and any combinations thereof.
  • compositions described herein are administered to a subject by appropriate administration routes, including, but not limited to, oral, parenteral (e.g., intravenous, subcutaneous, intramuscular) , intranasal, buccal, topical, rectal, or transdermal administration routes.
  • parenteral e.g., intravenous, subcutaneous, intramuscular
  • intranasal e.g., buccal
  • topical e.g., topical, rectal, or transdermal administration routes.
  • the pharmaceutical formulations described herein include, but are not limited to, aqueous liquid dispersions, liquids, gels, syrups, elixirs, slurries, suspensions, self-emulsifying dispersions, solid solutions, liposomal dispersions, aerosols, solid oral dosage forms, powders, immediate release formulations, controlled release formulations, fast melt formulations, tablets, capsules, pills, powders, dragees, effervescent formulations, lyophilized formulations, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate and controlled release formulations.
  • compositions including compounds described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof are manufactured in a conventional manner, such as, by way of example only, by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping, or compression processes.
  • compositions for oral use are obtained by mixing one or more solid excipient with one or more of the compounds described herein, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores.
  • Suitable excipients include, for example, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methylcellulose, microcrystalline cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose; or others such as polyvinylpyrrolidone (PVP or povidone) or calcium phosphate.
  • disintegrating agents are added, such as the cross-linked croscarmellose sodium, polyvinylpyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.
  • dyestuffs or pigments are added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.
  • compositions that are administered orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol.
  • the push-fit capsules contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers.
  • suitable liquids such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In some embodiments, stabilizers are added.
  • compositions for parental use are formulated as infusions or injections.
  • the pharmaceutical composition suitable for injection or infusion includes sterile aqueous solutions, or dispersions, or sterile powders comprising a compound described herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof.
  • the pharmaceutical composition comprises a liquid carrier.
  • the liquid carrier is a solvent or liquid dispersion medium comprising, for example, water, saline, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like) , vegetable oils, nontoxic glyceryl esters, and any combinations thereof.
  • the pharmaceutical compositions further comprise a preservative to prevent growth of microorganisms.
  • Disclosed herein are methods of treating a disease or disorder associated with SARS-COV-2 using a compound disclosed herein, or a pharmaceutically acceptable salt, solvate, or stereoisomer thereof, in combination with an additional therapeutic agent.
  • the additional therapeutic agent is administered at the same time as the compound disclosed herein. In some embodiments, the additional therapeutic agent and the compound disclosed herein are administered sequentially. In some embodiments, the additional therapeutic agent is administered less frequently than the compound disclosed herein. In some embodiments, the additional therapeutic agent is administered more frequently than the compound disclosed herein. In some embodiments, the additional therapeutic agent is administered prior than the administration of the compound disclosed herein. In some embodiments, the additional therapeutic agent is administered after the administration of the compound disclosed herein.
  • the additional therapeutic agent is an interferon, such as interferon alpha, or a pegylated interferon, such as PEG-intron or Pegasus.
  • this combination provides a greater clinical benefit than dosing either the interferon, pegylated interferon or the compound disclosed herein alone. Examples of greater clinical benefits include a larger reduction in symptoms, a faster time to alleviation of symptoms, reduced lung pathology, a larger reduction in the amount of SARS coronavirus in the patient (viral load) , and decreased mortality.
  • the SARS coronavirus infects cells which express p-glycoprotein.
  • the compounds disclosed herein are p-glycoprotein substrates.
  • compounds which inhibit the SARS coronavirus which are also p-glycoprotein substrates are dosed with a p-glycoprotein inhibitor.
  • p-glycoprotein inhibitors include verapamil, vinblastine, ketoconazole, nelfinavir, ritonavir, and cyclosporine.
  • the p-glycoprotein inhibitors act by inhibiting the efflux of the compounds disclosed herein out of the cell.
  • the inhibition of the p-glycoprotein based efflux will prevent reduction of intracellular concentrations of the compound disclosed herein due to p-glycoprotein efflux. Inhibition of the p-glycoprotein efflux will result in larger intracellular concentrations of the compound disclosed herein.
  • dosing a SARS coronavirus infected patient with the compound disclosed herein and a p-glycoprotein inhibitor lower the amount of the compound disclosed herein required to achieve an efficacious dose by increasing the intracellular concentration of the compound disclosed herein.
  • agents that may be used to increase the exposure of a mammal to a compound disclosed herein are those that can inhibit at least one isoform of the cytochrome P450 (CYP450) enzymes.
  • the isoforms of CYP450 that may be beneficially inhibited include, but are not limited to, CYP1A2, CYP2D6, CYP2C9, CYP2C19 and CYP3A4.
  • the compounds disclosed herein include compounds that are CYP3A4 substrates and are metabolized by CYP3A4.
  • dosing a SARS coronavirus infected patient with a compound that is a CYP3A4 substrate and a CYP3A4 inhibitor, such as ritonavir, nelfinavir or delavirdine, will reduce the metabolism of the compound by CYP3A4. This will result in reduced clearance of the compound and increased plasma concentrations. In some embodiments, the reduced clearance and higher plasma concentrations result in a lower efficacious dose of compound disclosed herein.
  • Example 1a (894.7 mg, 37.28%yield) was obtained as a white solid.
  • LCMS: (M+H) 525.1.
  • Example 1b (1.23 g, 51.44%yield) was obtained.
  • HPLC Retention time: 2.485 min, 10-80AB_4min.
  • SFC Retention time: 5.743 min, OD-3_IPA (DEA) _5_40_25ML.
  • Example 2a (40 mg, 22.22%yield) as a white solid.
  • Peak 2 was further purified by pre-HPLC (column: Boston Green ODS 150*30mm*5 ⁇ m; mobile phase: [water (FA) -ACN] ; B%: 50%-80%, 6min) to afford Example 2b (35 mg, 19.44%yield) as a white solid.
  • Peak 1 was further purified by prep-HPLC (column: Boston Green ODS 150*30mm*5 ⁇ m; mobile phase: [water (FA) -ACN] ; B%: 50%-80%, 6min) to give Example 4a (10.34 mg, 9.52%yield) .
  • LCMS: (M+H) 572.9.
  • Example 5a (77.13 mg) was obtained.
  • LCMS: (M+H) 565.2.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 8.98 (s, 1H) , 8.46 (s, 2H) , 8.38 -8.30 (m, 1H) , 7.99-6.66 (m, 3H) , 6.64 -6.45 (m, 1H) , 5.96 (s, 1H) , 3.89 -3.72 (m, 1H) , 2.10 -1.63 (m, 8H) , 1.54 -1.27 (m, 2H) , 1.04 -0.88 (m, 2H) , 0.64 -0.50 (m, 1H) .
  • Example 5b (78.57 mg) was obtained.
  • LCMS: (M+H) 565.2, SFC: Retention time: 2.033 min, OJ-H_EtOH (DEA) _5_40_25ML.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 8.97 (s, 1H) , 8.47 -8.34 (m, 3H) , 7.99 -6.62 (m, 3H) , 6.54 -6.39 (m, 1H) , 6.03 (s, 1H) , 3.88 -3.80 (m, 1H) , 2.05 -1.70 (m, 8H) , 1.60 -1.42 (m, 1H) , 1.36 -1.22 (m, 1H) , 1.08 -0.84 (m, 2H) , 0.71 -0.55 (m, 1H) .
  • Example 6a (44.61 mg, 45.61%yield, peak 1) was obtained.
  • LCMS (M+H) 549.1.
  • LCMS: (M+H) 549.0.
  • SFC Retention time: 2.552 min, OD_3_EtOH_DEA_5_40_25ML_7MIN.
  • Example 7a (36.16 mg) was obtained.
  • LCMS: (M+H) 543.0.
  • Example 7b (87.50 mg) was obtained.
  • LCMS: (M+H) 543.1.
  • Example 8a (138 mg) was obtained.
  • LCMS: (M+H) 541.1.
  • Example 8b (74 mg) was obtained.
  • LCMS: (M+H) 541.1.
  • HNMR: (400MHz, DMSO-d 6 ) : ⁇ 9.04 -8.95 (m, 1H) , 8.52 (s, 2H) , 8.35 (d, J 7.5 Hz, 1H) , 7.71 -7.28 (m, 4H) , 6.69 -6.48 (m, 1H) , 6.03 (s, 1H) , 3.80 (br s, 1H) , 2.01 -1.71 (m, 6H) , 1.53 -1.41 (m, 1H) , 1.40 -1.29 (m, 1H) .
  • Example 9a (25.53 mg) was obtained.
  • LCMS: (M+H) 559.1.
  • Example 9b (11.26 mg) was obtained.
  • LCMS: (M+H) 558.9.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 9.12 -8.92 (m, 1H) , 8.72 -8.30 (m, 3H) , 8.24 -6.93 (m, 3H) , 6.87 -6.57 (m, 1H) , 6.00 (s, 1H) , 3.82 (br s, 1H) , 2.22 -1.68 (m, 6H) , 1.56 -1.18 (m, 2H) .
  • Example 10a (10.96 mg, 19.57%yield) was obtained.
  • LCMS: (M+H) 526.0.
  • Example 10b (10.96 mg, 19.57%yield) was obtained.
  • LCMS: (M+H) 526.0.
  • Example 11a (11.83 mg) was obtained.
  • LCMS: (M+H) 491.0.
  • Example 11b (10.04 mg) was obtained.
  • LCMS: (M+H) 491.0.
  • Int-12-1 (4 g, 32.37 mmol) in ethyl formate (20 mL) was added TEA (9.83 g, 97.10 mmol, 13.52 mL) .
  • TEA 9.83 g, 97.10 mmol, 13.52 mL
  • the mixture was stirred at 80 °C for 16 hrs.
  • the mixture was diluted with DCM (20 mL) , filtered, and concentrated under vacuum.
  • Int-12-2 (3.73 g, crude) was obtained as a colorless oil.
  • Example 12a (47.55 mg) was obtained.
  • LCMS: (M+H) 477.0.
  • Example 12b (43.24 mg) was obtained.
  • LCMS (M+H) 477.0.
  • Example 12c (46.62 mg) was obtained as a white solid.
  • LCMS: (M+H) 477.0.
  • Example 12d (48.72 mg) was obtained.
  • LCMS: (M+H) 477.0.
  • Example 13a (1.54 mg) was obtained.
  • LCMS: (M+H) 543.0., SFC: Retention time: 1.804 min, OD_3_EtOH_DEA_5_40_25ML_7MIN.
  • Example 13b (1.89 mg, 11.81%yield) was obtained.
  • LCMS: (M+H) 543.0.
  • Example 14a (64.44 mg) was obtained.
  • LCMS: (M+H) 487.1.
  • Example 14b (81.43 mg) was obtained.
  • LCMS: (M+H) 487.2.
  • Example 15a (95.43 mg) was obtained.
  • LCMS: (M+H) 453.1.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 9.22 -9.12 (m, 1H) , 8.80 -8.62 (m, 2H) , 8.58 -7.96 (m, 1H) , 7.51 -7.24 (m, 2H) , 7.11 -6.78 (m, 3H) , 5.82 -5.61 (m, 1H) , 3.96 -3.77 (m, 3H) , 3.71 -3.58 (m, 1H) , 3.57 -3.47 (m, 1H) , 3.42 -3.34 (m, 2H) , 2.91 -2.88 (m, 1H) , 2.75 -2.52 (m, 1H) , 1.85 -1
  • Example 18a (20.13 mg, 25.81%yield) was obtained.
  • LCMS: (M+H) 524.1.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 9.09 (s, 1H) , 8.70 -8.42 (m, 3H) , 8.32 (br s, 2H) , 7.60 (br s, 4H) , 6.69 -6.42 (m, 1H) , 6.24 (s, 1H) , 3.83 (br s, 1H) , 2.15 -1.64 (m, 6H) , 1.59 -1.27 (m, 2H) .
  • Example 18b (8.8 mg, 11.28%yield) was obtained.
  • LCMS: (M+H) 524.0.
  • Example 19a (42.72 mg, 20.69%yield) was obtained.
  • LCMS: (M+Na) 530.1.
  • Example 19b (59.72 mg, 29.81%yield) was obtained as a white solid.
  • LCMS: (M+Na) 530.0.
  • Example 20a (9.04 mg, 16.41%yield) was obtained.
  • LCMS: (M+H) 480.0.
  • 1 H NMR 400 MHz, DMSO-d 6 ) : ⁇ 9.00 (br s, 1H) , 8.82 (br s, 1H) , 8.67 (br s, 1H) , 8.55 (br s, 2H) , 8.02 -7.65 (m, 2H) , 7.44 (brs, 2H) , 7.07 (br s, 1H) , 6.70 -6.43 (m, 1H) , 6.03 (br s, 1H) , 4.09 (br s, 1H) , 2.92 (br s, 3H) , 1.38 -0.97 (m, 1H) .
  • Example 20b (11.80 mg, 21.85%yield) was obtained.
  • HPLC Retention time: 3.020 min, 10-80AB_8min. lcm.
  • SFC Retention time: 4.688 min, C2_MEOH_DEA_5_40_28ML_6MIN.
  • Example 21a (5.46 mg, 24.07%yield) was obtained.
  • LCMS: (M+Na) 502.1.
  • Example 21b (6.02 mg, 27.61%yield) was obtained.
  • LCMS: (M+Na) 502.0.
  • 1 H NMR (400 MHz, DMSO-d 6 ) ⁇ 8.85 -8.71 (m, 1H) , 8.67 -8.64 (m, 1H) , 8.63 -8.59 (m, 1H) , 8.51 -8.48 (m, 1H) , 8.47 -8.42 (m, 1H) , 7.86 -7.72 (m, 2H) , 7.70 -7.31 (m, 2H) , 7.07 -7.01 (m, 1H) , 6.71 -6.47 (m, 1H) , 6.30 -6.18 (m, 1H) , 4.16 -3.98 (m, 1H) , 2.99 -2.80 (m, 2H) , 2.47
  • Int-22-4 (200 mg, 393.77 ⁇ mol) was purified by SFC (column: Phenomenex-Cellulose-2 (250mm *30mm, 10 ⁇ m) ; mobile phase: [Neu-MeOH] ; B%: 40%-40%, min) and purified by prep-HPLC (column: Boston Green ODS 150 *30mm *5 ⁇ m; mobile phase: [water (FA) -ACN] ; B%: 30%-70%, 6min) to afford four fractions.
  • SFC columnumn: Phenomenex-Cellulose-2 (250mm *30mm, 10 ⁇ m) ; mobile phase: [Neu-MeOH] ; B%: 40%-40%, min
  • prep-HPLC columnumn: Boston Green ODS 150 *30mm *5 ⁇ m; mobile phase: [water (FA) -ACN] ; B%: 30%-70%, 6min
  • Example 22a (28 mg) was obtained.
  • LCMS: (M+H) 508.1.
  • Example 22b (60 mg) was obtained.
  • LCMS: (M+H) 508.1.
  • HNMR: (400 MHz, DMSO-d 6 ) ⁇ 9.14 -9.08 (m, 1H) , 9.04 -8.95 (m, 1H) , 8.74 (br s, 1H) , 8.59 -8.47 (m, 2H) , 8.40 -8.32 (m, 1H) , 7.77 -7.07 (m, 4H) , 6.68 -6.40 (m, 1H) , 6.14 -5.95 (m, 1H) , 3.31 (br s, 1H) , 1.82 -1.66 (m, 1H) , 1.61 -1.50 (m, 2H) , 1.28 -1.10 (m, 1H) .
  • 19 F NMR (376 MHz, DMSO-d 6 ) ⁇ -125.97 --136.53 (m, 2F) , -141.92
  • Example 22c (40 mg) was obtained.
  • LCMS: (M+H) 508.1.
  • 19 F NMR (376 MHz, DMSO-d 6 ) ⁇ -131.07 ⁇ -133.
  • Example 22d (40 mg) was obtained.
  • LCMS: (M+H) 508.1.
  • Example 23a (2.25 mg) was obtained.
  • LCMS: (M+H) 536.1.
  • 1 H NMR (400 MHz, DMSO-d 6 ) ⁇ 9.10 (s, 1H) , 8.96 (s, 1H) , 8.60 -8.44 (m, 3H) , 8.34 (s, 1H) , 8.02 -6.70 (m, 4H) , 6.62 -6.35 (m, 1H) , 6.06 (s, 1H) , 3.75 (br s, 1H) , 2.40 -2.19 (m, 2H) , 2.14 -1.86 (m, 2H) , 1.78 -1.47 (m, 3H) , 1.32 -1.14 (m, 1H) .
  • Example 23b (1.35 mg) was obtained.
  • LCMS: (M+H) 536.1.
  • Example 23c (5.55 mg) was obtained.
  • LCMS: (M+H) 536.1.
  • Example 23d (8.94 mg) was obtained.
  • LCMS: (M+H) 536.1.
  • Example 23e (5.05 mg) was obtained.
  • LCMS: (M+H) 536.0.
  • Example 23f (9.02 mg) was obtained.
  • LCMS: (M+H) 536.0.
  • Example 23g (12.06 mg) was obtained.
  • LCMS: (M+H) 536.0 SFC: Retention time: 5.040min, AD_3_IPA_DEA_5_40_25ML.
  • Example 23h (5.50 mg) was obtained.
  • LCMS: (M+H) 536.0.
  • Example 24a (17 mg) was obtained.
  • LCMS: (M+H) 522.1.
  • Example 24b (14 mg) was obtained.
  • LCMS: (M+H) 508.1.
  • Example 24c (4 mg) was obtained.
  • LCMS: (M+H) 522.1.
  • Example 24d (23 mg) was obtained.
  • LCMS: (M+H) 522.0.
  • Example 25a (6.04 mg) was obtained.
  • LCMS: Retention time: (M+H) 488.2.
  • Example 25b (13.68 mg) was obtained.
  • LCMS: (M+H) 488.0.
  • Example 26a (70 mg) was obtained.
  • LCMS: (M+H) 470.0.
  • Example 26b (70 mg) was obtained as a white solid.
  • LCMS: (M+H) 470.0.
  • Example 42a (30 mg, 14.95%yield) was obtained.
  • LCMS (M+H) 575.2.
  • SFC Retention time: 2.220 min, OD-3_EtOH (DEA) _5_40_25ML.
  • Example 42b (40 mg, 19.71%yield) was obtained.
  • LCMS (M+H) 575.0.
  • SFC Retention time: 4.729 min, OD-3_EtOH (DEA) _5_40_25ML.
  • Example A In vitro assay (SARS-CoV-2 M pro enzymatic assay)
  • the C-His6-tagged SARS-CoV-2 MPRO (NC_045512) was cloned, expressed in E. coli and purified by WuXi.
  • the final concentrations of the Mpro protein and substrate were 25 nM and 25 ⁇ M, respectively, in the MPRO enzymatic assay.
  • Reference compound GC376 was provided by WuXi AppTec and was included in each plate to ensure assay robustness. Test compounds were tested at single dose or 10 doses titration, in duplicate. Compounds were added to an assay plate (384w format) using ECHO, in duplicate wells. The final concentration is 10 ⁇ M for the single dose experiment. As for the full dose response experiment, samples were 3-fold serially diluted starting from 25 ⁇ M for 10 doses and added to an assay plate, in duplicate wells. The final concentrations ( ⁇ M) of each compound was 25, 8.33, 2.778, 0.926, 0.309, 0.103, 0.034, 0.011, 0.0038, and 0.0013.
  • REU fluorescence signal
  • the inhibition activity was calculated using the formula below, IC50 values were calculated using the Inhibition%data.
  • Inhibition% ( (CPD -BGHPE) - (ZPE -BGZPE) ) / ( (HPE -BGHPE) - (ZPE -BGZPE) ) ⁇ 100 where, HPE is high percent effect controls (1 ⁇ M of GC376 + enzyme + substrate) ; ZPE is zero percent effective controls (enzyme + substrate, no compound) ; CPD is compound activity testing wells (compound + enzyme + substrate; and BG is background control wells (no enzyme) .
  • IC 50 values of compounds were calculated with the GraphPad Prism software using the nonlinear regression model of log (inhibitor) vs. response -variable slope (four parameters) .
  • Example M pro IC 50 1a A 1b C 2a A 2b C 3a A 3b C 4a B 4b C 5a C 5b B
  • IC50 (nM) 0 ⁇ A ⁇ 100; 100 ⁇ B ⁇ 1,000; 1,000 ⁇ C ⁇ 10,000; 10,000 ⁇ D
  • Example B In vitro antiviral cell-based assay (live SARS-CoV-2)
  • cell lines can be used depending on customer’s request. Also, different variants of concern (e.g. alpha, gamma, kappa and delta etc. ) can be included according to the customer’s request.
  • cells will be infected by 0.1 MOI SARS-CoV-2 for 1h. After that, the infectious inoculum will be replaced with drug-containing medium which is serial-diluted (7 concentrations) .
  • the culture supernatants of the SARS-CoV-2-infected cells are harvested at 48 h post-inoculation (hpi) for qRT-PCR analysis of viral RNA load.
  • a total of 140 ⁇ L of culture supernatant will be lysed with 560 ⁇ L of AVL buffer, which is subsequently extracted for total RNA with the QIAamp viral RNA mini kit (Qiagen, Hilden, Germany) .
  • Qiagen the QIAamp viral RNA mini kit
  • qRT-PCR will be used for quantitation of SARS-CoV-2 replication using the QuantiNova Probe RT-PCR kit (Qiagen) with a LightCycler 480 Real-Time PCR System (Roche) .
  • Each 20 ⁇ L reaction mixture is 10 ⁇ L of 2 ⁇ QuantiNova Probe RT-PCR Master Mix, 1.2 ⁇ L of RNase-free water, 0.2 ⁇ L of QuantiNova Probe RT-Mix, 1.6 ⁇ L each of 10 ⁇ M forward and reverse primer, 0.4 ⁇ L of 10 ⁇ M probe, and 5 ⁇ L of extracted RNA as the template. Reactions will be incubated at 45 °C for 10 min for reverse transcription, 95 °C for 5 min for denaturation, follow by 45 cycles of 95 °C for 5 s and 55 °C for 30 s. Signal detection and measurement are taken in each cycle after the annealing step. The cycling profile end with a cooling step at 40 °C for 30 s.
  • the primers and probe sequences are against the RNA-dependent RNA polymerase/helicase (RdRP/Hel) gene region of SARS-CoV-2: Forward primer: 5′-CGCATACAGTCTTRCAGGCT-3′; Reverse primer: 5′-GTGTGATGTTGAWATGACATGGTC-3′; specific probe: 5′-FAM TTAAGATGTGGTGCTTGCATACGTAGAC-IABkFQ-3′.
  • RdRP/Hel RNA-dependent RNA polymerase/helicase
  • VeroE6 cells will be seeded at 2 ⁇ 105 cells/well in 24-well tissue culture plates on the day before carrying out the assay. After 24 h of incubation, 50 plaque-forming units (PFU) of SARS-CoV-2 will be added to the cell monolayer and the plates are further incubated for 1 h at 37 °C in 5%CO2 before removal of unbound viral particles by aspiration of the media and washing once with DMEM.
  • PFU plaque-forming units
  • Monolayers are then overlaid with media containing 1.5%low melting agarose (Cambrex Corporation, East Rutherford, NJ, USA) in DMEM and appropriate concentrations of individual compound (5 concentrations) , inverted and incubated as above for another 72 h.
  • the wells are then fixed with 10%formaldehyde (BDH, Merck, Darmstadt, Germany) overnight.
  • BDH, Merck 0.7%crystal violet
  • the percentage of plaque inhibition relative to the control (i.e., without the addition of compound) wells will be determined for each antiviral agent concentration.
  • EC50 was calculated using a sigma plot (SPSS) in an Excel add-in ED50V10. The plaque reduction assay experiments are performed in triplicate.
  • IC50 (nM) 0 ⁇ A ⁇ 100; 100 ⁇ B ⁇ 1,000; 1,000 ⁇ C ⁇ 10,000; 10,000 ⁇ D

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne des composés, des compositions pharmaceutiques et des Méthodes de traitement d'une infection par le SRAS-CoV-2.
PCT/CN2022/128937 2021-11-02 2022-11-01 Inhibiteurs de sras-cov-2 pour le traitement d'infections à coronavirus WO2023078238A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CNPCT/CN2021/128237 2021-11-02
CN2021128237 2021-11-02
CN2022117029 2022-09-05
CNPCT/CN2022/117029 2022-09-05

Publications (1)

Publication Number Publication Date
WO2023078238A1 true WO2023078238A1 (fr) 2023-05-11

Family

ID=86240660

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2022/128937 WO2023078238A1 (fr) 2021-11-02 2022-11-01 Inhibiteurs de sras-cov-2 pour le traitement d'infections à coronavirus

Country Status (1)

Country Link
WO (1) WO2023078238A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11731944B2 (en) 2021-11-02 2023-08-22 Insilico Medicine Ip Limited SARS-CoV-2 inhibitors for treating coronavirus infections

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170313685A1 (en) * 2016-04-28 2017-11-02 Purdue Research Foundation Broad-spectrum non-covalent coronavirus protease inhibitors
WO2021219089A1 (fr) * 2020-04-30 2021-11-04 Insilico Medicine Ip Limited Inhibiteurs de sras-cov-2 ayant des modifications covalentes pour le traitement d'infections à coronavirus
CN113845440A (zh) * 2021-11-04 2021-12-28 沈阳药科大学 基于ugi反应合成的非天然肽类idh1抑制剂及其制法和应用
WO2022119756A1 (fr) * 2020-12-04 2022-06-09 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions et méthodes d'inhibition de l'activité protéase mpro et de prévention et de traitement d'une infection à sars-cov-2

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170313685A1 (en) * 2016-04-28 2017-11-02 Purdue Research Foundation Broad-spectrum non-covalent coronavirus protease inhibitors
WO2021219089A1 (fr) * 2020-04-30 2021-11-04 Insilico Medicine Ip Limited Inhibiteurs de sras-cov-2 ayant des modifications covalentes pour le traitement d'infections à coronavirus
WO2022119756A1 (fr) * 2020-12-04 2022-06-09 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions et méthodes d'inhibition de l'activité protéase mpro et de prévention et de traitement d'une infection à sars-cov-2
CN113845440A (zh) * 2021-11-04 2021-12-28 沈阳药科大学 基于ugi反应合成的非天然肽类idh1抑制剂及其制法和应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JON JACOBS, VALERIE GRUM-TOKARS, YA ZHOU, MARK TURLINGTON, S. ADRIAN SALDANHA, PETER CHASE, AIMEE EGGLER, ERIC S. DAWSON, YAHIRA M: "Discovery, Synthesis, And Structure-Based Optimization of a Series of N -( tert -Butyl)-2-( N -arylamido)-2-(pyridin-3-yl) Acetamides (ML188) as Potent Noncovalent Small Molecule Inhibitors of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) 3CL Protease", JOURNAL OF MEDICINAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 56, no. 2, 24 January 2013 (2013-01-24), US , pages 534 - 546, XP055333594, ISSN: 0022-2623, DOI: 10.1021/jm301580n *
STILLE JULIA, JEVGENIJS TJUTRINS, GUANYU WANG, FELIPE A. VENEGAS, CHRISTOPHER HENNECKER, ANDRES MAURICIO RUEDA, CAITLIN E. MIRON, : "Design, synthesis and biological evaluation of novel SARS-CoV-2 3CLpro covalent inhibitors.", CHEMRXIV, 1 April 2021 (2021-04-01), pages 1 - 31, XP093062258, Retrieved from the Internet <URL:https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/60c756ec337d6ce5f9e28f35/original/design-synthesis-and-biological-evaluation-of-novel-sars-co-v-2-3c-lpro-covalent-inhibitors.pdf> [retrieved on 20230710] *
YAMANE DAIKI, SATSUKI ONITSUKA, SUYONG RE, HIKARU ISOGAI, RUI HAMADA, TADANARI HIRAMOTO, EIJI KAWANISHI, KENJI MIZUGUCHI, NAOYA SH: "Selective Covalent Targeting of SARS-CoV-2 Main Protease by 2 Enantiopure Chlorofluoroacetamide ", CHEMRXIV, 28 September 2021 (2021-09-28), XP093062257, Retrieved from the Internet <URL:https://chemrxiv.org/engage/api-gateway/chemrxiv/assets/orp/resource/item/6152f9b3aade361750c52ada/original/selective-covalent-targeting-of-sars-co-v-2-main-protease-by-enantiopure-chlorofluoroacetamide.pdf> [retrieved on 20230710] *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11731944B2 (en) 2021-11-02 2023-08-22 Insilico Medicine Ip Limited SARS-CoV-2 inhibitors for treating coronavirus infections

Similar Documents

Publication Publication Date Title
JP6954567B2 (ja) ２−置換芳香族環−ピリミジン系誘導体及びその調製と医学的用途
CN105283451B (zh) 新的病毒复制抑制剂
WO2015048567A1 (fr) Modulateurs ebi2 spirocycliques
JP2007504120A (ja) アダマンタン誘導体およびアザビシクロオクタン誘導体およびアザビシクロノナン誘導体、ならびにこれらの調製方法およびｄｐｐ−ｉｖ阻害剤としてのこれらの使用
WO2022020730A1 (fr) Inhibiteurs de hsd17b13 quinazolinone et leurs utilisations
US11780854B2 (en) Prolyl hydroxylase domain-containing protein (PHD) inhibitors and uses thereof
US11999713B2 (en) Methionine adenosyltransferase 2a (MAT2A) inhibitors and uses thereof
WO2018086546A1 (fr) Dérivé pyrimidine à cycle aromatique 2-polysubstitué et préparation et utilisation médicale
WO2023078238A1 (fr) Inhibiteurs de sras-cov-2 pour le traitement d&#39;infections à coronavirus
KR20230069984A (ko) Tyk2 억제제 및 그의 용도
WO2022103960A1 (fr) Inhibiteurs de dichlorophénol hsd17b13 et utilisations de ceux-ci
WO2023078231A1 (fr) Inhibiteurs du sars-cov-2 pour le traitement d&#39;infections à coronavirus
WO2022216627A1 (fr) Inhibiteurs d&#39;hsd17b13 de type thiazole/isothiazole et leurs utilisations
WO2021178362A1 (fr) Modulateurs d&#39;assemblage de capside de l&#39;hépatite b
WO2023155892A1 (fr) Inhibiteurs de kinase (pkmyt1) inhibitrice de cdc2 spécifique de la tyrosine et de la thréonine associés à la membrane et leurs utilisations
WO2023143520A1 (fr) Inhibiteurs d&#39;ectonucléotide pyrophosphatase-phosphodiestérase 1 (enpp1) et leurs utilisations
EP4320111A1 (fr) Inhibiteurs d&#39;hsd17b13 de type hydroxypyridine et leurs utilisations
KR20160149561A (ko) 5-ht7 수용체에 작용하는 바이페닐 유도체, 이의 제조방법 및 이를 유효성분으로 포함하는 약학조성물
WO2023165528A1 (fr) Inhibiteurs de la diacylglycérol kinase (dgk) alpha et leurs utilisations
KR20240089791A (ko) 메티오닌 아데노실트랜스퍼라제 2a (mat2a) 억제제 및 그의 용도
WO2023174397A1 (fr) Inhibiteurs de kinase inhibitrice de cdc2 spécifique de la tyrosine et de la thréonine associés à une membrane de pyrazole (pkmyt1) et leurs utilisations
WO2024094150A1 (fr) Inhibiteurs de l&#39;inflammasome nlrp3 et leurs utilisations
WO2024141015A1 (fr) Inhibiteurs de protéine tyrosine phosphatase et leurs utilisations
CN117402185A (zh) 三嗪类化合物及其在药物中的应用
WO2024061300A1 (fr) Inhibiteurs de trex1 et utilisations associées

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22889262

Country of ref document: EP

Kind code of ref document: A1