WO2023041074A1 - 样本分析仪、样本分析仪的控制方法 - Google Patents
样本分析仪、样本分析仪的控制方法 Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
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- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Definitions
- the present application relates to the technical field of medical equipment, in particular to a sample analyzer and a control method for the sample analyzer.
- the embodiment of the present application provides a method for controlling a sample analyzer, including:
- the reagent dispensing device is used for dispensing reagents into the cuvette
- Figure 2 is a schematic block diagram of a sample analyzer in one embodiment
- Fig. 21 is a schematic diagram of the reaction curve in another embodiment of the embodiment of the present application.
- the mixing mechanism 15 is used to mix the reaction liquid in the cuvette that needs to be mixed.
- the quantity of mixing mechanism 15 can be one or more.
- the reaction part 16 has at least one placement position, which is used for placing the cuvette and incubating the reaction solution in the cuvette.
- the reaction part 16 can be a reaction disc, which is arranged in a disc-shaped structure and has one or more placement positions for placing cuvettes.
- the reaction disc can rotate and drive the cuvettes in the placement positions to rotate, for Schedule the cuvettes in the reaction tray and incubate the reaction solution in the cuvettes.
- the detection device 17 is used to perform optical measurement on the incubated reaction solution to obtain the reaction data of the sample. In one embodiment, the detection device 17 is separately disposed outside the reaction component 16 .
- the following mainly takes the sample analyzer as a biochemical analyzer as an example for description.
- the cuvette and the liquid in the cuvette will rotate with the reaction disc, and will pass through the photometer every fixed time, and the photometer will collect a signal in each corresponding detection cycle, that is, the light intensity signal of one point is collected,
- the absorbance data of one point can be obtained through processing; the same cuvette and the liquid in the cuvette will pass through the photometer multiple times as the reaction disk rotates, and the absorbance data of multiple points corresponding to multiple detection cycles can be obtained.
- the absorbance data are combined to obtain a response curve.
- the inventors of the present application have found that the existing signal collection method only collects the absorbance data of a certain position of the cuvette when the cuvette passes through the photometer, and there will be abnormal interference in the cuvette or the liquid in the cuvette, such as foreign objects (bubbles) or solid foreign matter), uneven reaction solution, stains, scratches, residual water on the surface of the cuvette, or electromagnetic interference to the signal of the cuvette, and some interferences often only appear in the cuvette or part of the liquid in the cuvette;
- the photometer may be aligned with the position of the foreign matter in the cuvette, and the obtained absorbance data cannot effectively judge the real state of the cuvette and the liquid in the cuvette, and cannot The accuracy of the measurement results obtained from the absorbance data is guaranteed.
- the sample analyzer of the embodiment of the present application includes a sampling device 310, a reaction device 320, and a detection device 330
- the detection device 330 includes a light source 331, an optical signal acquisition component 332, a memory 341, and a processor 342, wherein the optical signal
- the collection component 332 includes, for example, a photoelectric sensor.
- the light source 331 and the optical signal collection component 332 may be the light source and photoelectric sensor in a photometer.
- the sampling device 310 is used to aspirate samples from the sample containers dispatched to the sampling position, and deliver the aspirated samples to the cuvette 321 .
- the sampling device 310 has a suction nozzle 311 for aspirating a predetermined volume of sample from the sample tube assigned to the sampling position through the suction nozzle 311 and delivering the sample to the cuvette 321 .
- the reaction device 320 has a cup position for accommodating a cuvette 321, and the reagent and the sample are mixed in the cuvette 321 to prepare a reaction liquid; and incubating, the reaction solution is prepared from the reagent and the sample.
- the sample analyzer further includes a reagent dispensing device 350 for dispensing reagents into the cuvettes.
- the light source irradiates the cuvette with light from the light source
- the optical signal acquisition component of the detection device collects signals of the cuvette and the liquid in the cuvette, and stores the collected signal
- the intensity of the signal and the time of the signal collection are used to obtain a signal sequence for subsequent analysis and identification.
- the optical signal acquisition component is used for full-time signal acquisition
- the memory is used for storing the full-time signals collected by the optical signal acquisition component
- the processor processes the collected full-time signals, and can also output analysis results.
- the analysis result may include the measurement result of the sample, and may also include the state of the cuvette or the liquid in the cuvette or the detection device in the sample analyzer, but of course it is not limited thereto.
- the optical signal acquisition components corresponding to one or more wavelengths in the detection device continue to collect full-time signals; of course, it is not limited to this, for example, multiple wavelengths in the detection device
- the corresponding optical signal acquisition components collect signals at different times, and the signals collected by the optical signal acquisition components corresponding to multiple wavelengths can form the full-time signal, and can also realize full-time signal acquisition.
- the cuvette moves relative to the light source and continuously irradiates.
- the cuvette passes the light source multiple times. Each time the cuvette passes the light source and the duration of the light source continuously irradiating the cuvette constitutes a detection cycle. Passing the light source each time constitutes multiple detection cycles, and the optical signal acquisition component acquires multiple signal sequences of the cuvette during the multiple detection cycles.
- the single-point absorbance corresponding to the signal sequence is calculated through the interpolation algorithm; the single-point absorbance is determined according to the signal sequence, compared with the signal of one point in each detection period and the determination of the corresponding absorbance.
- the accuracy of the absorbance obtained in each detection cycle can be improved.
- the reaction curve can be determined according to the single-point absorbance of multiple detection cycles, and the measurement results of the sample can be obtained according to the information such as the amplitude and rate of change of the reaction curve.
- the sample analyzer may further include a control device, for example, the control device may control the actions of various devices in the sample analyzer according to the analysis results.
- the analysis result may include the measurement result of the sample, and may also include the state of the cuvette or the liquid in the cuvette or the detection device in the sample analyzer, but of course it is not limited thereto.
- the processing of the signal sequence by the processor includes, based on the signal sequence, the processor determines whether the cuvette or the liquid in the cuvette or the liquid in the sample analyzer Check the status of the device.
- the liquid in the reaction cup can be a reagent or a sample, and can also be a reaction liquid.
- the state of the cuvette or the liquid in the cuvette or the detection device in the sample analyzer is judged.
- the signal characteristics corresponding to the signal sequence include but are not limited to at least one of the following: the boundary time point (such as the start time point and the end time point) of each subsequence in the signal sequence, the duration, and the intensity of the signal in each subsequence Characteristic values (such as maximum value, minimum value, average value, etc.), fluctuations of subsequences, and slopes of signal strengths in subsequences.
- the signal sequence of the cuvette in each detection cycle includes at least one signal subsequence.
- the signal subsequence includes a baseline signal subsequence 110 and a cuvette signal 120 .
- the value of the baseline signal subsequence 110 obtained by the same cuvette in different lighting periods, that is, the detection period, can be defined as A1, A2, A3, ..., An in turn, as shown in the baseline signal subsequences of the five detection periods in Figure 6
- the values of sequence 110 can be defined as A1 to A5 in sequence.
- the cuvette signal 120 includes signal subsequences collected by the optical signal collection component when the cuvette is in the detection area of the optical signal collection component, and the characteristic waveform of the cuvette signal 120 may be typical or irregular.
- the cuvette signal 120 includes a rising edge signal subsequence 112 , a cuvette signal subsequence 113 , and a falling edge signal subsequence 115 .
- 111 is the start time point of cuvette signal 120, after the start time point 111 is the rising edge signal subsequence 112 of cuvette signal 120; after the rising edge signal subsequence 112, the reaction
- the cuvette signal subsequence 113 of the cuvette signal 120 remains stable, or sometimes fluctuates; after that is the cuvette signal 120 falling edge signal subsequence 115 , 116 is the end time point of the cuvette signal 120 .
- the optical signal acquisition component of the detection device When the cuvette moves relative to the detection device, the optical signal acquisition component of the detection device first collects the signal at one side of the cuvette wall, and this section of the signal can be called the rising edge signal subsequence 112 . Afterwards, the optical signal acquisition component collects the signal of the light-transmitting surface of the cuvette and the liquid in the cuvette or collects the signal when the cuvette is empty. Generally, it is a relatively stable value, such as B; the signal value of the cuvette signal subsequence 113 can determine the value of the cuvette signal 120 , for example, the value of the cuvette signal 120 is determined to be B.
- Each cuvette has its own value B of the cuvette signal 120, and the cuvette signal 120 values obtained by the same cuvette in different lighting periods, that is, different detection periods, can be defined as B1, B2, B3, ... , Bn, as shown in Figure 6, the values of the cuvette signal 120 in five detection periods can be defined as B1 to B5 in sequence.
- the optical signal collection component collects a signal at the other side of the cuvette wall, and this section of the signal may be called a falling edge signal subsequence 115 .
- the wave width 114 of the cuvette signal 120 can be determined according to the time length between two signal points of the cuvette signal 120 , and the signal intensity of the two signal points is less than or equal to the value B of the cuvette signal. For example, it is determined according to the time length between the time when the signal strength of the signal point in the rising edge signal subsequence 112 rises to a preset value and the time when the signal strength of the signal point in the falling edge signal subsequence 115 drops to a preset value , the preset value is less than or equal to the value B of the cuvette signal 120; of course, it is not limited thereto, as shown in FIG.
- the time length between is determined as the wave width 114 of the cuvette signal 120 .
- the signal characteristics corresponding to the signal sequence include the duration of at least one signal subsequence and/or the boundary time point of at least one signal subsequence, and the signal subsequence can be the rising edge signal subsequence 112, the falling edge signal Subsequence 115 or cuvette signal subsequence 113 , cuvette signal subsequence 113 is located between rising edge signal subsequence 112 and falling edge signal subsequence 115 .
- the boundary time point of the rising edge signal subsequence 112 includes the start time point 111 of the cuvette signal 120, the duration of the rising edge signal subsequence 112 can be called the rising edge duration, and the cuvette signal subsequence 113
- the duration can be determined according to the wave width 114 of the cuvette signal 120, the duration of the falling edge signal subsequence 115 can be called the falling edge duration, and the boundary time point of the falling edge signal subsequence 115 can be the end time of the cuvette signal 120 Point 116.
- the signal sequence acquired in each detection cycle can be applied to deal with the abnormal reaction process.
- the reaction between the sample and the reagent will follow a certain reaction kinetic principle.
- the value A of the cuvette external signal i.e., the value A of the baseline signal subsequence 110
- the value B of the cuvette signal 120 or the signal characteristics of the cuvette signal 120 can be compared with their corresponding set thresholds, and then Determine whether the reaction process conforms to the law.
- the processor determines that the optical signal acquisition component is abnormal when the signals of the baseline signal subsequence do not meet the first preset condition.
- the value A of the baseline signal subsequence is determined according to the intensity of at least one signal in the baseline signal subsequence, and when the value A of the baseline signal subsequence exceeds a preset range, it is determined that the optical signal acquisition component is abnormal.
- the abnormality of the optical signal acquisition component includes, but is not limited to, that the optical signal acquisition component is affected by electromagnetic interference to measure.
- the external signal of the cuvette that is, the baseline signal subsequence 110 does not participate in the detection of the reaction process of the cuvette
- the value A of the baseline signal subsequence 110 should be Fluctuates within a small range, for example, the intensity A of each signal in the baseline signal subsequence 110 should be smaller than the threshold RA. If the intensity A of a signal in the baseline signal subsequence 110 is greater than or equal to the threshold RA, it can be determined that the measurement of the optical signal acquisition component is abnormal. For example, when the measurement of the optical signal acquisition component is abnormal, the sample analyzer can retest according to the set process, or perform automatic maintenance on the sample analyzer, or issue an alarm.
- the processor can compare the signal sequences of multiple detection cycles to determine whether there is an abnormality in the movement of the cuvette relative to the detection device. Different detection cycles of different cuvettes can also be compared with different detection cycles of different cuvettes.
- the signal sequence of each detection period includes at least one signal subsequence
- the processor judges whether the duration of the signal subsequence or the change of the characteristic time point of the signal subsequence meets the second preset condition Is there any abnormality in the relative motion.
- the signal characteristics related to the cuvette signal such as the starting time point 111 of the cuvette signal 120, the duration of the rising edge of the rising edge signal subsequence 112, and the waveform of the cuvette signal 120
- the width 114, the duration of the falling edge of the falling edge signal subsequence 115, and the end time point 116 of the cuvette signal 120 reflect whether the signal of the reaction process of the sample and the reagent is normal, and can be used for monitoring the reaction process, for example, for judging Whether there is any abnormality in the movement of the reaction cup relative to the detection device, such as whether the movement of the reaction cup is uniform.
- the reaction cup is loaded on the reaction disk, and the reaction disk rotates at a certain relatively stable speed, so that the reaction cup rotates relative to the detection device, and the signal is collected through the optical signal collection component.
- the movement of the cuvette relative to the detection device is normal, such as when the reaction disc loaded with the cuvette moves normally (such as running at a constant speed), for the same cuvette, in different detection periods, the duration of the same signal subsequence The deviation should be less than the corresponding threshold; when the duration of the signal subsequence in different detection periods for the same cuvette is less than the corresponding threshold, it can be determined that the movement of the cuvette relative to the detection device is normal; when there are different detection periods
- the deviation between the durations of the signal subsequences is greater than a corresponding threshold, it can be determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the sample analyzer may
- the cuvette moves relative to the detection device normally;
- the deviation between durations of the rising edges of the period is greater than the threshold b, it can be determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the deviations between the wave widths 114 of the cuvette signals 120 of different detection periods are all smaller than the threshold c, it can be determined that the cuvette moves relative to the detection device normally;
- the deviation between the wave widths 114 of the cup signal 120 is greater than the threshold c, it can be determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the cuvette moves relative to the detection device normally;
- the deviation between the durations of the falling edges of the cycle is greater than the threshold d, it can be determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the timer is reset to zero once in each detection cycle and timing is performed after reset to obtain the acquisition time of each signal in the signal sequence, including the The boundary time point of the signal subsequence; for example, the timer is reset to zero once at the start time point of the cuvette signal 120 of each detection cycle;
- the deviation between the boundary time points of the signal subsequences should also be smaller than the corresponding threshold.
- the start time 111 of the cuvette signal 120 collected in different detection periods, and the deviation between the start times 111 of different detection periods should be less than the threshold a.
- the deviation between the start times 111 of different detection cycles is less than the threshold a, it can be determined that the movement of the cuvette relative to the detection device is normal; when there is a deviation between the start times 111 of different detection cycles If it is greater than the threshold a, it is determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the deviations between the end time points 116 of the cuvette signals 120 of different detection periods are all smaller than the threshold e, it can be determined that the cuvette moves relative to the detection device normally;
- the deviation between the end time points 116 is greater than the threshold e, it can be determined that there is an abnormality in the movement of the cuvette relative to the detection device.
- the full-time signal acquisition method can obtain signal sequences of multiple different cuvettes.
- the signal sequence is used to monitor the detection process of the sample analyzer.
- the processor can compare the signal sequences of a plurality of continuous cuvettes, and the continuous plurality of cuvettes pass through the detection device in turn; according to the interval between the characteristic time points of the signal subsequences in the two consecutive signal sequences , if the interval between the boundary time points meets the third preset condition, it is judged whether there is an abnormality in the relative movement of the cuvette.
- the optical signal acquisition component of the detection device successively collects the signals of the two cuvettes and the liquid in the cuvettes, and converts the signal The intensity and corresponding time are stored to obtain two signal sequences as shown in Fig. 8 for subsequent analysis and identification.
- the wave width 114 of the cuvette signal in each signal sequence can be called the inner width I of the cuvette, and the signal width 202 between specific positions of the cuvette can be called the inter-cup width J;
- the interval between the boundary time points of the same signal subsequence of the cuvettes such as the interval between the start time points of the rising edge signal subsequences of two adjacent cuvettes.
- the reaction cup is loaded on the reaction disk, and the reaction disk rotates at a certain relatively stable speed, and collects signals through the optical signal collection component.
- the wave width 114 cup inner width I
- the interval 202 inter-cup width J
- the boundary time points of the signal subsequence in the signal sequence should fluctuate within the corresponding preset range, otherwise it can be determined that the relative motion between the cuvette and the detection device is abnormal, such as the working state of the reaction disc is abnormal .
- the inner width I of a certain cuvette exceeds the preset range [m1, n1], it can be determined that the relative movement of the cuvette is abnormal, and the optical signal collection of this cuvette is abnormal. For example, it can also be determined that the cuvette The current test is invalidated and retested.
- the inner width I of multiple cuvettes exceeds the preset range [m1, n1]
- it can be determined that the relative movement of the cuvettes is abnormal for example, it is determined that the movement of the reaction disc is unstable, prompting that the instrument needs to be inspected and maintained .
- the inter-cup width J of multiple cuvettes exceeds the range [m2, n2], it can be determined that the relative movement of the cuvettes is abnormal, for example, it is determined that the movement of the reaction plate is unstable, prompting that the instrument needs to be inspected and maintained.
- the processor can compare the signal sequences of multiple detection cycles of the same cuvette containing liquid to determine whether the reaction process of the liquid in the cuvette is abnormal.
- the processor can compare the signal sequences of multiple detection cycles of the same cuvette containing the reaction solution to determine whether there is any abnormality in the reaction process of the reaction solution.
- it is the signal sequence of 5 detection cycles of the same cuvette containing the reaction solution, each of which includes at least one signal subsequence, such as including a rising edge signal subsequence, a cuvette signal subsequence, a falling along at least one of the signal subsequences.
- the processor obtains the target signal strength of the detection cycle according to the signal in the signal subsequence of each detection cycle, compares the change parameters of the target signal strength of the multiple detection cycles, and based on the obtained Change parameters to judge whether there is abnormality in the reaction process. For example, it is judged whether there is an abnormality in the reaction process according to whether the change parameters of the target signal strength in the multiple detection cycles meet the fifth preset condition.
- the signals of different detection periods The deviation of the subsequence, such as the change parameter of the target signal intensity, satisfies a preset threshold condition, such as being smaller than the threshold x, or greater than the threshold y.
- a preset threshold condition such as being smaller than the threshold x, or greater than the threshold y.
- the change parameter of the target signal strength (such as the value B2 of the cuvette signal subsequence) and the target signal strength of the first detection cycle (such as the value B1 of the cuvette signal subsequence) should be smaller than the threshold x.
- the reaction starts.
- This stage can be called the sample reaction stage, and the collected signals are gradually changing. Enhancement; the change parameter between the target signal strength of the third detection cycle and the target signal strength of the second detection cycle, and the change parameter of the target signal strength of the fourth detection cycle and the target signal strength of the third detection cycle should be greater than Threshold y.
- the reaction end stage the change of the target signal intensity of the fifth detection cycle and the target signal intensity of the fourth detection cycle The parameter should be less than the threshold z.
- the stage corresponding to the at least two detection cycles is the stage of not adding samples
- the change parameter of the target signal strength in the at least two detection cycles is greater than the threshold x
- it can be determined that there is an abnormality in the reaction process and it can be executed retest.
- the stage corresponding to the at least two detection cycles is the sample reaction stage
- the change parameter of the target signal strength in the at least two detection cycles is smaller than the threshold y
- the stage corresponding to the at least two detection cycles is the reaction end stage
- the change parameter of the target signal strength in the at least two detection cycles is greater than the threshold z, it can be determined that there is an abnormality in the reaction process, and a retest can be performed.
- the sample reaction stages corresponding to the at least two detection cycles determine the change parameter thresholds corresponding to the sample reaction stages, and according to the at least two detection cycles
- the comparison result of the change parameter of the target signal intensity in each detection cycle and the change parameter threshold determines whether there is an abnormality in the reaction process.
- the same cuvette has the same abnormality in multiple measurement items, for example, when the same cuvette is used to detect different items or different samples, the same abnormality occurs multiple times, it can be determined that the cuvette is fouled, and the sample analyzer Automatic maintenance can be performed, or an alarm can be issued.
- the same cuvette performs two reaction processes of different measurement items to obtain two corresponding reaction solutions.
- the processor compares the multiple signal sequences of the two reaction solutions, if the multiple signal sequences of the two reaction solutions are at the same stage, the change parameters are all abnormal, for example, the processor compares For the change parameters of the respective target signal intensities of the two reaction solutions, if the change parameters of the target signal intensities are abnormal at the same stage of the two reaction solutions, it indicates that the cuvette is fouled; for example, the same If the cuvette performs the reaction process of two different measurement items, if there is an abnormality in the stage of not adding the sample, both in the sample reaction stage, or both in the end of the reaction stage, it indicates that the cuvette is fouled.
- the signal mutation features include peak subsequences or valley subsequences on the cuvette signal subsequence.
- a peak subsequence 123 and/or a valley subsequence 133 may appear on the cuvette signal subsequence 113 .
- the signal in the cuvette signal subsequence 113 fluctuates, and the signal of the toggling part is greater than the value B of the cuvette signal subsequence 113, it is determined that the fluctuating part is the peak subsequence 123, and the peak subsequence 123
- the peak can be denoted as C; for example, the peak C of the peak subsequence 123 is greater than the threshold Rc. If there are multiple peak subsequences, the values of the multiple peak subsequences can be defined as C1, C2, C3, . . . , Cn in sequence.
- the characteristic waveform of the crest subsequence of the cuvette signal can be typical or irregular.
- the starting time point of the crest subsequence 123 can be expressed as 121. If there are multiple crest subsequences, there are multiple crest subsequences 123 corresponding to the starting time point 121; the rising edge duration of the crest subsequence 123 is 122 , if there are multiple crest subsequences, there are multiple crest subsequences corresponding to the duration of the rising edge; the duration of the falling edge of the crest subsequence 123 is 125, if there are multiple crest subsequences, there are multiple crest subsequences The duration of the falling edge corresponding to the sequence; the end time point of the crest subsequence 123 is 126, if there are multiple crest subsequences, then there are multiple crest subsequences corresponding to the end time point.
- the wave width 124 of the peak subsequence 123 When determining the wave width 124 of the peak subsequence 123, two signal points are determined on the peak subsequence 123, and the values of these two signal points are less than or equal to the peak value C of the peak subsequence 123, and according to the two signal points of the peak subsequence 123
- the time length between the signal points determines the width 124 of the peak subsequence 123; that is, the width 124 of the peak subsequence 123 refers to the time length of a certain signal point less than or equal to the peak value C of the waveform of the peak subsequence 123.
- the signal in the cuvette signal subsequence 113 fluctuates, and the signal value of the fluctuating part is smaller than the value B of the cuvette signal subsequence 113, it is determined that the fluctuating part is the valley subsequence 133, and the wave valley subsequence 133 A value, such as a valley value, can be expressed as D; for example, the valley value D of the valley subsequence 133 is smaller than the threshold Rd. If there are multiple valley subsequences, the values of the multiple valley subsequences can be defined as D1, D2, D3, . . . , Dn in sequence.
- the characteristic waveform of the valley sub-sequence of the cuvette signal can be typical or irregular.
- the starting time point of the valley subsequence 133 can be expressed as 131, if there are multiple valley subsequences, then there are multiple valley subsequences corresponding starting time points; the falling edge duration of the valley subsequence 133 is 132, if If there are multiple valley subsequences, there are multiple rising edge durations corresponding to the valley subsequences; the rising edge duration of the valley subsequence 133 is 135, if there are multiple valley subsequences, there are multiple valley subsequences corresponding to The corresponding duration of the falling edge; the end time point of the trough subsequence 133 is 136, if there are multiple trough subsequences, then there are multiple trough subsequences corresponding to the duration of the falling edge.
- the wave width 134 of the valley subsequence 133 When determining the wave width 134 of the valley subsequence 133, two signal points are determined on the valley subsequence 13, and the values of these two signal points are greater than or equal to the valley value D of the valley subsequence 133, and according to the valley value D of the valley subsequence 13
- the time length between two signal points determines the wave width 134 of the valley subsequence 133; that is, the wave width 134 of the valley subsequence 133 refers to the time length of a certain signal point greater than or equal to the valley value D of the waveform of the valley subsequence 133.
- whether there is an abnormality can be determined by the number of peak subsequences or valley subsequences of the signal sequence of the same cuvette in multiple detection cycles, for example, the same cuvette has a peak subsequence or a wave in multiple detection cycles. If the number of grain sequences is greater than the preset threshold, it can be determined that the cuvette is abnormal or the liquid in the cuvette is abnormal.
- the processor can compare the signal sequences of multiple detection periods to determine whether the same signal mutation feature occurs in the same time period, and if so, the signal mutation feature is a state judgment feature.
- the same signal mutation feature may include substantially similar peak subsequences or valley subsequences, wherein the similarity of the substantially similar peak subsequences is greater than or equal to the similarity threshold, and the similarity of the substantially similar valley subsequences is greater than Or equal to the similarity threshold.
- the signal sequences of the two detection periods have basically similar peak subsequences and valley subsequences in the same time period of their respective detection periods, and the peak subsequence and/or valley subsequence can be determined as the state judgment feature .
- the optical signal acquisition component of the detection device collects signals from the same position of the cuvette. For example, if the peak subsequences 123 of multiple detection periods, the deviation of their starting point time 121 (such as CS) is less than the threshold Rcs, and the deviation of their end time 126 (such as CE) is less than the threshold Rce, it can be determined that the The peak subsequences 123 of multiple detection cycles appear in the same time period, that is, appear at the same position of the cuvette; the deviation of the rising edge duration 122 (such as CH) of the peak subsequences 123 of the multiple detection cycles is less than the threshold Rch, the deviation of their peak subsequence width 124 (such as CL) is less than the threshold Rcl, and the deviation of their falling edge duration 125 (such as CD) is less than the threshold Rcd, then the peak subsequence 123 of the plurality of detection cycles can be determined are substantially similar peak subs
- the valley subsequences 133 of a plurality of detection periods the deviation of their starting point time 131 (such as DS) is less than the threshold Rds, and the deviation of their end time 136 (such as DE) is less than the threshold Rde, it can be determined that the The valley subsequence 133 of multiple detection cycles appears in the same time period, that is, it appears at the same position of the cuvette; the deviation of the falling edge duration 132 (such as DD) of the valley subsequence 133 of the multiple detection cycles is less than the threshold Rdd, the deviation of their valley subsequence width 134 (as DL) is less than the threshold Rd1, and the deviation of their rising edge duration 135 (as DH) is less than the threshold Rdh, then the valley subsequence 133 of the plurality of detection cycles can be determined are substantially similar valley subsequences, and the valley subsequences 133 of the plurality of detection periods are determined as state judgment
- the processor can also count the number of occurrences of the state judgment feature in multiple detection cycles of the same cuvette to obtain the count, and compare the count with a statistical threshold to determine whether the cuvette or the The liquid in the cuvette or the state of the detection device in the sample analyzer.
- the state judging feature of the same cuvette is greater than the statistical threshold, it can be determined that the state judging feature is the state judging feature of a certain cuvette, and it can be determined that the reaction
- the cuvette is abnormal or the detection device is determined to be abnormal, wherein the cuvette abnormality includes staining of the cuvette (such as dirt or scratches) or discoloration of the cuvette, and the abnormal state of the detection device includes abnormality of the light source or optical signal acquisition component, etc.;
- the state judgment feature in the same cuvette is less than or equal to the statistical threshold, it can be determined that the state judgment feature is an occasional state judgment feature, and it is determined that the reaction liquid is abnormal, such as the presence of liquid in the cuvette Foreign matter (such as lipid particles) or uneven mixing of the liquid in the cuvette, etc., or confirm that there is foreign matter in the cuvette.
- the reagent when the sample is detected, can be added to the cuvette first, and then the sample can be added to the cuvette, and the sample and the reagent are mixed to form a reaction solution.
- the state of the reagent in the cuvette such as the pH value of the reagent
- the status of the reagent determines whether there is any abnormality in the reagent, for example, the reagent bottle has been opened for too long, the reagent has expired or the reagent has deteriorated, etc.; optionally, when the reagent is not put in, the status of the cuvette can be determined through the signal sequence, such as whether the cuvette is There are scratches, whether the cleanliness of the cuvette is up to standard, or whether there is residual liquid in the cuvette; optionally, the liquid in the cuvette can be a reaction solution prepared from rea
- the processor compares the signal sequences of multiple different detection cycles of the same cuvette, and the signal sequence is the signal sequence before adding samples and/or reagents to the cuvette; if the cuvette adds The statistical times of the sample and/or reagent previous state judgment features (such as peak subsequence or valley subsequence) are less than or equal to the statistical threshold, suggesting that there is foreign matter in the cuvette, such as residual water or impurities (such as dust, confetti, etc.); if the statistical number of state judgment features is greater than the statistical threshold, it can be determined and prompted that the cuvette is fouled, such as the cuvette has scratches.
- the statistical times of the sample and/or reagent previous state judgment features such as peak subsequence or valley subsequence
- residual water or impurities such as dust, confetti, etc.
- the sample analyzer can be controlled to The cuvette is cleaned; of course it is not limited thereto, for example, when it is determined that the signal sequence of the cuvette before the cuvette is added with the sample and/or reagent appears the crest subsequence 123 and/or the valley subsequence 133, this reaction may not be performed.
- the cuvette can be tested, or the automatic maintenance of the instrument can be performed, or an alarm can be given for the abnormality of the cuvette cleaning module.
- the processor can also compare the signal sequences of multiple different detection cycles of the same cuvette, and the signal sequence is the signal sequence after the sample and/or reagent is added to the cuvette. For example, after adding samples and/or reagents to the cuvette, when the number of occurrences of the status judging feature in the same cuvette is greater than the statistical threshold, it can be determined that the status judging feature is a specific status judging feature , and determining that the cuvette is abnormal or determining that the detection device is abnormal, wherein the abnormality of the cuvette includes staining of the cuvette (such as dirt or scratches) or discoloration of the cuvette, and the abnormal state of the detection device includes a light source or light Abnormality of the signal collection component, etc.; after adding samples and/or reagents to the cuvette, when the number of occurrences of the status judging feature in the same cuvette is less than or equal to the statistical threshold, it can be determined that the status judging feature is sporadic
- the state judging feature is used to
- the sample analyzer such as a biochemical analyzer
- detection wavelengths such as 8, 12 or 16 wavelengths.
- FIG. 16 and FIG. 17 during the continuous irradiation of the same cuvette by the light source assembly, one or more detection cycles (two detection cycles as shown in FIG. 17) parallel acquisition and parallel
- the analog-to-digital conversion process obtains the photoelectric data (or can be called signal sequence) of n wavelengths respectively, and the photoelectric data corresponding to each wavelength includes the signal sequence of one or more detection cycles, and the signal sequence of each detection cycle includes all The corresponding relationship between the intensity and time of at least two of the signals at the wavelength.
- the signal sequence corresponding to each wavelength may be called a wavelength subsequence.
- the detection device collects multi-wavelength full-time photoelectric data of one or more detection periods of the cuvette to obtain a multi-wavelength signal sequence as shown in FIG. 17 .
- the signal sequence can also be referred to as an optical signal pulse waveform, and the signal sequence can be a graph formed by a pulse including signal strength and time information, or it can include a corresponding relationship between signal strength and time information data array.
- the signal sequence of all wavelengths of the same cuvette in the same detection period may prompt that there is a foreign object in the cuvette or there is a scratch in the cuvette or If the light source or optical signal acquisition component is abnormal, etc., it can also control the maintenance of the instrument or send an alarm message to the user, or automatically arrange for retesting.
- the same abnormality such as a peak subsequence or a valley subsequence
- the changes of the peak sub-sequence or the valley sub-sequence in different detection periods to determine whether the foreign matter in the cuvette is a bubble or a solid foreign matter; for example, when a peak appears in the signal sequence of multiple detection periods of each wavelength Subsequence, and the difference between the value C of the peak subsequence of the previous detection cycle and the value C of the peak subsequence of the next detection cycle is greater than or equal to the preset threshold r, it can be determined and prompted that there are bubbles in the reaction solution;
- the difference between the value C of the peak subsequence of the previous detection cycle and the value C of the peak subsequence of the next detection cycle is less than the preset threshold r, which can determine and prompt the presence of solid foreign matter in the reaction solution; for example, when each wavelength A valley subsequence appears in the signal sequence of multiple detection periods, and the difference between the value D of the valley subsequence of the previous detection period and the value
- the signal sequence of the same cuvette in all parts of the same detection period has the same abnormality, for example, a peak subsequence or If the abnormality of the valley sub-sequence does not appear in the same position of the signal sequence of other wavelengths, it can be determined and prompted that the liquid to be tested in the reaction solution is not uniform; it can also control the maintenance of the instrument or prompt the liquid in the cuvette ( The liquid in the cuvette can be reagent or reaction solution) is not uniform, or it can also automatically arrange re-testing.
- the same abnormality for example, a peak subsequence or If the abnormality of the valley sub-sequence does not appear in the same position of the signal sequence of other wavelengths, it can be determined and prompted that the liquid to be tested in the reaction solution is not uniform; it can also control the maintenance of the instrument or prompt the liquid in the cuvette ( The liquid in the cuvette can be reagent or reaction solution) is not uniform, or it can also automatically arrange re-testing.
- the processor may perform state monitoring according to the photoelectric data corresponding to at least two wavelengths. Exemplarily, it may be determined whether the signal sequence of each wavelength has the state judgment feature; of course, it is not limited thereto.
- the processor compares the signal sequences of multiple detection cycles of the same cuvette, and obtains the number of occurrences of signal mutation features in the signal sequences of multiple detection cycles of the same cuvette, specifically, one or more The number of occurrences of the signal mutation characteristics corresponding to each wavelength; when the number of occurrences of the signal mutation characteristics in the signal sequences of multiple detection cycles of the same cuvette is greater than the preset statistical threshold, and the signal sequences of all wavelengths, that is, the cuvette All wavelength subsequences have signal mutation characteristics, which can indicate abnormal detection devices (such as abnormal optical signal acquisition components or light sources) or staining of cuvettes; When the number of occurrences is greater than the preset statistical threshold, and the signal sequences of part of the wavelengths, that is, part of the wavelength subsequences of the cuvette, all have signal mutation characteristics, which can prompt the cuvette to change color.
- the processor compares the signal sequences of multiple detection cycles of the same cuvette, and the signal sequence is the signal sequence after adding samples and/or reagents to the cuvette, and obtains the signal sequences of the multiple detection cycles
- the number of times that the state judgment feature appears in the signal sequence of the signal sequence specifically, the number of times that the state judgment feature corresponding to one or more wavelengths appears can be obtained, and the statistical number of the state judgment feature is obtained; when the state judgment feature of the same cuvette appears
- the number of times that is, the number of statistics is greater than the statistical threshold, and all wavelength subsequences have state judgment features, which can prompt the abnormality of the detection device (such as the abnormality of the optical signal acquisition component or the light source) or the fouling of the cuvette; when the same reaction
- the number of occurrences of the state judging feature of the cuvette is greater than the statistical threshold, and the state judging feature appears in part of the wavelength subsequences, it may prompt the cuvette to change color.
- the processor compares the signal sequences of multiple detection cycles of the same cuvette, and obtains the number of occurrences of signal mutation features in the signal sequences of multiple detection cycles of the same cuvette, specifically, one or more The number of occurrences of the signal mutation characteristics corresponding to each wavelength; when the number of signal mutation characteristics occurrences in the signal sequences of multiple detection cycles of the same cuvette is less than or equal to the preset statistical threshold, and the signal sequences of all wavelengths, that is, the All wavelength subsequences of the cuvette have signal mutation characteristics, which can prompt the liquid in the cuvette, such as the presence of foreign matter in the reaction solution; when the number of signal mutation characteristics in the signal sequences of multiple detection cycles of the same cuvette is less than or is equal to the preset statistical threshold, and the signal sequences of part of the wavelengths, that is, part of the wavelength subsequences of the cuvette, all have signal mutation characteristics, which can determine and prompt that the reaction solution is not mixed uniformly.
- the processor compares the signal sequences of multiple different detection periods of the same cuvette, and the signal sequence is the signal sequence after adding the sample and/or reagent to the cuvette, and obtains the multiple detection cycles.
- the number of occurrences of the state judgment feature in the periodic signal sequence can specifically acquire the number of occurrences of the state judgment feature corresponding to one or more wavelengths, and obtain the statistical number of the state judgment feature.
- the number of occurrences of the state judgment feature in the same cuvette that is, the number of statistics is less than or equal to the statistical threshold, and all wavelength subsequences have the state judgment feature, it can prompt the liquid in the cuvette, such as the presence of foreign body.
- the state judgment feature in the same cuvette that is, the statistical number is less than or equal to the statistical threshold, and the state judgment feature appears in some wavelength subsequences, it can be determined and prompted that the reaction solution is not mixed uniformly; optionally, It can also prompt for inspection and maintenance of samples, instruments, and/or can also automatically schedule retesting.
- the processor compares multiple signal sequences of multiple cuvettes, counts the number of occurrences of the signal mutation feature, and obtains the statistical number of the signal mutation feature; specifically, one or The number of occurrences of signal mutation features in the signal sequence of multiple detection cycles, or the number of occurrences of signal mutation characteristics in the signal sequence corresponding to one or more wavelengths of each cuvette can be obtained; for example, multiple The number of occurrences of the signal mutation feature in the signal sequence corresponding to the different wavelengths of the detection cycle is obtained to obtain the statistical number of the state judgment feature. If the number of statistics is greater than the statistical threshold, it is determined that the detection device is abnormal, such as the light source or the optical signal acquisition component is abnormal.
- the processor compares multiple signal sequences of multiple cuvettes, and the signal sequence is a signal sequence after adding samples and/or reagents to the cuvettes, and counts the number of occurrences of the state judgment feature, Obtain the statistical number of the state judgment feature; specifically, the number of occurrences of the state judgment feature in the signal sequence of one or more detection cycles of each cuvette can be obtained, or one or more of each cuvette can be obtained.
- the number of occurrences of the state judgment feature in the signal sequence corresponding to the wavelength for example, the number of occurrences of the state judgment feature in the signal sequence corresponding to different wavelengths of multiple detection cycles of each cuvette can be obtained, and the statistical number of the state judgment feature can be obtained.
- the detection device is abnormal, such as the light source or the optical signal acquisition component is abnormal.
- the detection device is abnormal, such as the light source or the optical signal acquisition component is abnormal.
- the signal sequences of the samples and reagents in a plurality of continuous cuvettes have peak subsequences or valley subsequences, it can be determined that the light source or the optical signal collection component is unstable.
- the signal sequence of multiple cuvettes fluctuates regularly, it is determined that the state of the light source lamp and the optical signal acquisition component is abnormal.
- the value C of the crest subsequence 123 exceeds the threshold Rc in the signal sequences of a plurality of consecutive cuvettes, or the value D of the valley subsequence 133 in the signal sequences of a plurality of continuous cuvettes is smaller than the threshold Rd, then it can be determined that The optical signal acquisition of several cuvettes is abnormal, for example, it is determined that the light source and optical signal acquisition components are unstable; it can also be determined that the current test of these cuvettes is invalid, the instrument can give an alarm, and it can also prompt that the light source needs to be replaced, or the optical signal acquisition component needs to be replaced. Components are inspected and repaired.
- the processor compares multiple signal sequences of multiple cuvettes, for example, compares the signal sequences of the respective detection cycles of a plurality of continuous cuvettes, and counts the number of occurrences of the state judgment feature, if the count is greater than the count threshold, and the deviation of the signal mutation amplitude corresponding to the state judgment feature in two adjacent cuvette signal sequences satisfies the fourth preset condition, and it is judged that the light source is abnormal.
- the signal mutation amplitude corresponding to the state judgment feature may include the value C of the peak subsequence 123 or the value D of the valley subsequence 133 .
- the value C of the peak subsequence 123 exceeds the threshold Rc in the signal sequences of a plurality of consecutive cuvettes, and the deviation between the values C of the peak subsequence 123 in the signal sequences of two adjacent cuvettes is smaller than the threshold x, or the value D of the valley subsequence 133 in multiple cuvettes is less than the threshold Rd, and the deviation between the values D of the valley subsequence 133 in the signal sequences of two adjacent cuvettes is less than the threshold y, then it can be determined that this If the optical signal collection of several cuvettes is abnormal, it can be determined that the light source is unstable; it can also be determined that the current test of these cuvettes is invalid, and the instrument can give an alarm and prompt that the light source needs to be replaced.
- the detection device has a code disc to provide a reference signal
- the optical signal acquisition component starts the signal acquisition of the cuvette according to the reference signal, and acquires a signal sequence, and the acquired signal sequence includes at least two signals; and/or the memory stores the sequence of signals according to the reference signal.
- the signal transition edge of each code tooth is aligned with one side of the corresponding cuvette.
- each code tooth transition 101 ′ generated during the rotation of the reaction disk can be monitored, and the signal acquisition of the corresponding cuvette is started according to each code tooth transition 101 ′. Since the optical signal acquisition component collects a plurality of signals of the cuvette in the one detection cycle, therefore, the code tooth signal edge of the photoelectric code disc does not need to be precisely aligned with the specific position (such as the center) of each cuvette, It only needs to be located before the center of the cuvette, so that the collected signal sequence includes the signal of the cuvette center, and the difficulty of parts processing, assembly and debugging can be greatly reduced.
- the code tooth signal edge of the photoelectric code disc only needs to play the role of determining the same cuvette; the peak, valley, pulse start point, pulse end point, and pulse width corresponding to the signal sequence of each wavelength are identified by combining algorithms , half-peak width, specific width, area, slope, rising edge time, falling time and other signal characteristics, the signal sequence is processed, such as determining the measurement result of the sample and/or judging the cuvette or the cuvette The state of the liquid or detection device in the sample analyzer.
- the device that generates the signal edge of the code tooth of the photoelectric code disc such as an optocoupler and a code disc; only through the method of signal processing, for example, collecting the signal sequence of the cuvette through the whole process of the light source, through waveform alignment, The acquisition of the signal value of the calculation result is realized, thereby saving hardware cost and debugging time.
- the required signal can be extracted from the signal sequence according to the signal edge of the code tooth of the photoelectric code disc, such as at least part of the signal of the cuvette signal subsequence.
- the optical signal acquisition component continuously collects signal sequences of multiple different cuvettes, and the memory continuously stores the signal sequences of the multiple cuvettes .
- a plurality of different cuvettes are placed on the reaction disk, the reaction disk can rotate and drive the plurality of different cuvettes to rotate, and the plurality of different cuvettes respectively pass through the detection device when rotating, so that the optical signal collection component collects The signal sequences of the multiple different cuvettes.
- the detection device may not have a code disc, for example, as long as the cuvette is continuously moving relative to the detection device, the cuvette is collected by the optical signal acquisition component signal sequence.
- the signal sequences of different cuvettes can be determined in the signal sequence collected by the optical signal collection component according to the baseline signal subsequence 110, or can be determined according to the rising edge signal subsequence 112 of the cuvette signal 120, the cuvette signal subsequence 113 At least one of the falling edge signal subsequence 115 determines the signal sequence of different cuvettes in the signal sequence collected by the optical signal collection component.
- the sample analyzer such as a biochemical analyzer
- the sample analyzer has multiple detection wavelengths, such as 8, 12 or 16 wavelengths.
- the light source assembly generates irradiating light, and the irradiating light irradiates a cuvette or the same position in the cuvette liquid, and the irradiating light can emit at least two light signals after irradiating the cuvette, at least two of the The wavelengths of the optical signals are different.
- the irradiating light irradiates a cuvette or liquid in the cuvette, and forms outgoing light after at least one of transmission, scattering and reflection of the cuvette or the liquid in the cuvette.
- the irradiating light 21 irradiates the cuvette 321 or the liquid in the cuvette, and forms outgoing light after being transmitted and/or scattered by the cuvette 321 or the liquid in the cuvette. Different sides of 321.
- the optical signal collection component 332 collects the outgoing light formed after being reflected by the cuvette or the cuvette liquid, that is, the light signal reflected by the cuvette or the cuvette liquid
- the optical signal collection component 332 and the light source component 331 can be arranged in the The same side of the cuvette 321.
- the irradiating light irradiates a cuvette or the same position in a cuvette liquid at the same time, and at least two of the light signals are collected simultaneously.
- the irradiation lights of different wavelengths irradiate a cuvette or the same position in a cuvette liquid at different times.
- FIG. 13 is a schematic diagram of the optical path between the light source assembly 331 and the optical signal collection assembly 332; optionally, the sample analyzer further includes a spectroscopic device 333, which splits the emitted light from the cuvette 321 into is an optical signal 23 of at least two wavelengths.
- the light source assembly 331 irradiates the cuvette 321 with mixed-wavelength light 21 (such as white light), the mixed-wavelength light 21 is transmitted and/or scattered by the cuvette 321 to form outgoing light 22, and the outgoing light 22 is split by the spectroscopic device 333 to form light of different wavelengths.
- the optical signal collection component 332 can collect the optical signals 23, for example, the optical signal collection component 332 includes a photoelectric sensor.
- the spectroscopic device 333 divides the output light of the cuvette 321 into optical signals 23 of at least two wavelengths.
- the spectroscopic device 333 can be called a rear spectroscopic device. of the same position.
- the light source can be a point light source, or a light source array in which multiple point light sources are arranged along the longitudinal axis of the cuvette.
- the collected signal reflects the
- the two-dimensional signal of the liquid in the cuvette has richer information, and by analyzing the data, the situation of the reaction process can be better grasped, and abnormalities can be identified more accurately and/or more abnormalities can be identified.
- the light source component may also include an optical component that changes the light transmission path, so that the light from the light source illuminates the cuvette.
- the light source assembly may include a single light source that emits white light.
- the cuvette rotates relative to the light source assembly, and the light source assembly is a light source array capable of emitting light signals of different wavelengths.
- each light source in the light source array is Arranged in the direction of movement; cuvettes pass through each point light source in the light source array in turn, and the wavelength of each point light source is different. Since each light source in the light source array is arranged along the moving direction of the cuvette, the same position in the cuvette will be irradiated by light sources of different wavelengths in turn, that is, the irradiation light of different wavelengths will irradiate the cuvette or the liquid in the cuvette. The time is different.
- the sample analyzer further includes a spectroscopic device, and the spectroscopic device divides the irradiation light generated by the light source assembly into at least two wavelengths of irradiation light, and the at least two wavelengths of irradiation light simultaneously irradiate a reaction
- the spectroscopic device can be called Front splitter.
- the light splitting device divides the illumination light generated by the light source assembly into at least two wavelengths of illumination light, and the illumination light of at least two wavelengths irradiates a cuvette or the same position in the cuvette liquid at different angles,
- the emission directions of the at least two wavelengths of irradiation light after irradiating the cuvette are different, and the at least two light signals are formed, so that the exit light of the cuvette or the liquid in the cuvette can be collected by a plurality of signal acquisition components, That is, the light signal, or an adjustment device for the light transmission direction, such as a light converging device, etc., can be set on the light output side to transmit the light to the same signal acquisition component for collection.
- the light source assembly includes at least two light sources, and the at least two light sources emit light with different wavelengths and simultaneously irradiate a cuvette or the same position in the liquid in the cuvette.
- the light source module can be equipped with multiple light sources. The wavelengths of the light emitted by the multiple light sources are different and the directions of the emitted light are different.
- the at least two light signals; then the exit light of the cuvette or the liquid in the cuvette can be collected through multiple signal collection components, that is, the light signal, or an adjustment device for the direction of light transmission can be set on the light exit side, such as light.
- a converging device, etc. transmits the light to the same signal acquisition component for acquisition.
- the front spectroscopic device can also divide the illumination light generated by the light source assembly into at least two wavelengths of illumination light, and the illumination light of at least two wavelengths irradiates a cuvette or the same position in the cuvette liquid in parallel.
- the at least two wavelengths of irradiation light can emit from the same position after irradiating the cuvette to form at least two light signals; optionally, when the cuvette rotates, the cuvette passes through the irradiation light of each wavelength in sequence, The same position in the cuvette will be irradiated by different wavelengths of light in turn, that is, the time for different wavelengths of light to irradiate the cuvette or the liquid in the cuvette is different; the signal acquisition component can collect at least two optical signals and process them Obtain at least two optoelectronic data.
- the optical signal collecting component 332 can collect at least two optical signals 23 of different wavelengths.
- the reaction cup will pass through the detection device periodically.
- the results of multiple wavelengths measured when the cuvette passes through the optical signal acquisition component once are not measured at the same time, but the time when the cuvette passes the detection device this time is segmented according to the number of wavelengths, and each period of time A signal acquisition of a specific wavelength is performed.
- the corresponding acquisition circuit block diagram of this multi-wavelength photoelectric data acquisition method is shown in Figure 14.
- multi-channel signal conditioning needs to be selected through signal channel selection, and one signal is selected for analog-to-digital conversion. Due to the large amount of photoelectric data, the prior art uses this relatively low-cost circuit to realize multi-wavelength detection.
- the samples added in the cuvette collect signals of different wavelengths at different time periods, and each small square represents the detection position of a corresponding wavelength, and the detection positions of different wavelengths are different.
- the problem with this prior art is that measurement signals of multiple wavelengths at the same time or in the same time period cannot be obtained at the same time, and abnormal processing cannot be performed by comparing signals with multiple wavelengths at the same time or in the same period, which is prone to abnormal results.
- the inventors of the present application also improved the multi-wavelength photoelectric data acquisition method, and controlled the light source assembly of the sample analyzer to generate irradiating light, and the irradiating light irradiated a cuvette or the same position in the cuvette liquid; at least two light sources were collected simultaneously. signal, the at least two optical signals are formed by the irradiation light irradiating the cuvette or the cuvette liquid, and the wavelengths of at least two optical signals are different; at least two optical signals are processed to obtain at least Two optoelectronic data.
- multiple optical signals of different wavelengths can be collected at the same time, and photoelectric data corresponding to multiple wavelengths can be obtained through processing.
- the detection positions and time periods of multiple wavelengths are the same.
- the time of the cuvette passing the light source assembly is no longer segmented according to the number of wavelengths, and a signal of a specific wavelength is collected for each period of time, but signals of multiple wavelengths are collected in parallel at the same time , the detection positions and time periods of multiple wavelengths are the same.
- the advantage of this solution is that the photoelectric data of multiple wavelengths at the same position at the same time can be obtained at the same time, and the photoelectric data between multiple wavelengths has reference significance.
- the detection device includes a light source assembly 331, an optical signal acquisition assembly 332, and an analog-to-digital conversion assembly 334.
- the light source assembly 331 generates irradiating light, and the irradiating light irradiates a cuvette at the same time.
- the optical signal acquisition assembly 332 collects at least two optical signals at the same time, and the at least two optical signals are irradiated by the irradiation light to the reaction After the cups are formed from the same position, at least two of the optical signals have different wavelengths; the analog-to-digital conversion component 334 performs parallel analog-to-digital conversion processing on at least two of the optical signals to obtain at least two photoelectric data.
- the photoelectric data is a signal sequence formed by the relationship between the intensity of the signal and the acquisition time, and the photoelectric data collected during the continuous irradiation of the same cuvette 321 by the light source assembly 331 includes signal sequences corresponding to at least two wavelengths , the signal sequence of the same wavelength includes a corresponding relationship between the intensity and time of at least two of the signals.
- the light source assembly 331 can irradiate different wavelengths of illumination light at different times, for example, the light source assembly 331 periodically switches the wavelength of the illumination light; for example, the light source assembly 331 may include a light source obtained by integrating multiple spectral signal sources , the spectrum of the irradiated light can be switched.
- the light source assembly 331 irradiates the irradiation light of different wavelengths at different times, and the optical signal collection assembly 332 collects the optical signals of the corresponding wavelengths at the corresponding time to collect multiple different wavelengths.
- the optical signal of multiple wavelengths is processed to obtain photoelectric data corresponding to multiple wavelengths.
- the detection positions of multiple wavelengths are also the same, and photoelectric data of multiple wavelengths at the same position can be obtained.
- the photoelectric data between multiple wavelengths has reference significance.
- the sample analyzer further includes a spectroscopic device 333, which splits the output light of the cuvette 321 into optical signals of at least two wavelengths.
- each corresponding wavelength signal channel has an independent signal acquisition channel, and each independent signal acquisition channel has a corresponding photoelectric sensor and a corresponding analog-to-digital conversion function.
- each wavelength signal channel can correspond to an analog-to-digital conversion device, or multiple signal channels can correspond to an analog-to-digital device, as long as the signal of each wavelength signal channel can be independently converted from analog to digital. . Since each wavelength signal channel has its own analog-to-digital conversion capability, the photoelectric data corresponding to multiple wavelengths can be collected at the same time.
- the cuvette moves relative to the light source assembly, for example, the cuvette rotates around the light source assembly;
- the photoelectric data is a signal sequence formed by the relationship between signal intensity and acquisition time.
- the photoelectric data collected during the continuous irradiation of the cuvette by the light source assembly includes signal sequences corresponding to at least two wavelengths, and the signal sequence of the same wavelength includes at least two signal sequences of the intensity and time of the signals.
- the signal sequence of one detection period of the same wavelength includes the intensity and time correspondence of at least 10 signals; preferably, the signal sequence of one detection period of the same wavelength includes the intensity and time correspondence of at least 50 signals relation. Please refer to FIG.
- the state monitoring based on the at least two photoelectric data includes: obtaining target detection items of the liquid in the cuvette irradiated by the light source assembly or the liquid in the cuvette irradiated by the light source assembly type; determine the target wavelength according to the target detection item or the type of the liquid, and the target wavelength can also be called the main wavelength; perform state monitoring according to the photoelectric data corresponding to the target wavelength, and the target detection item is in the cuvette
- the current detection item of the added sample, the type of liquid can be divided into reagent, sample, reaction liquid or cleaning liquid, etc.
- a certain wavelength or a certain range of wavelengths is more sensitive to certain detection items or certain types of liquids. For example, certain wavelengths have a more obvious detection effect on lipid particles, and a certain wavelength or a certain range of wavelengths can be selected.
- the wavelength is used as the target wavelength, and the state monitoring is carried out according to the photoelectric data corresponding to the target wavelength; by selecting different target wavelengths for different target detection items or the type of the liquid, the monitoring results of the state monitoring of the sample analyzer are more accurate. precise.
- the first signal subsequence/second signal subsequence corresponding to each wavelength of multiple detection cycles of the same cuvette can be extracted from the signal sequence corresponding to each wavelength corresponding to the same cuvette, and according to at least The first signal subsequence/second signal subsequence corresponding to two wavelengths generates response curves corresponding to at least two wavelengths; wherein, the response curve corresponds to a line segment in each detection cycle.
- Figure 5 is a response curve corresponding to a wavelength, wherein the response curve corresponding to each wavelength includes a plurality of line segments, and each line segment corresponds to the first signal subsequence/second signal subsequence of a detection cycle; optional, because The first signal subsequence/second signal subsequence is a section extracted from the signal sequence of each detection period, and the multiple line segments in the response curve can be made according to the time corresponding to each first signal subsequence/second signal subsequence. There is an interval between adjacent line segments, which can reflect the time corresponding to each first signal subsequence/second signal subsequence.
- each wavelength of multiple detection periods of the same cuvette corresponds to multiple first signal subsequences/second signal subsequences, and for each first signal subsequence/second signal subsequence, the interpolation algorithm, Calculate the single-point absorbance corresponding to the signal sub-sequence in this section by means of averaging, taking the median, etc.; determining the single-point absorbance according to the signal sub-sequence can improve the absorbance of each point compared with the absorbance of one point in each detection cycle. The accuracy of the absorbance obtained by the test cycle.
- certain wavelengths have obvious detection effects on interfering substances, such as lipid particles, and this wavelength can be used as an auxiliary wavelength to eliminate interference.
- this wavelength can be used as an auxiliary wavelength to eliminate interference.
- a certain wavelength or a certain range of wavelengths that are sensitive to the reaction solution can be selected as the target wavelength
- the photoelectric data of the target wavelength can be used to obtain preliminary results such as absorbance
- the photoelectric data of the auxiliary wavelength can be used to eliminate interference in the preliminary results to obtain the described
- the detection result of the liquid in the cuvette by using different target wavelengths for different target detection items, the obtained detection result of the liquid in the cuvette is more accurate.
- the processor can acquire first photoelectric data corresponding to a first wavelength and second photoelectric data of a second wavelength in at least two photoelectric data; The data is corrected to obtain the third photoelectric data.
- the acquiring the detection result of the liquid in the cuvette according to at least two of the photoelectric data includes: acquiring weights of at least two wavelengths, and acquiring at least two wavelengths according to the photoelectric data corresponding to at least two wavelengths the reactivity corresponding to each wavelength in the cuvette; obtain the reactivity of the sample added in the cuvette according to the weight and reactivity of each wavelength; obtain the detection result of the liquid in the cuvette according to the reactivity.
- the weights of the photoelectric data corresponding to different wavelengths may be determined according to the target detection items, but of course it is not limited thereto.
- the instrument when the state of the cuvette or the liquid in the cuvette or the detection device in the sample analyzer is abnormal, the instrument needs to be able to retest the sample, perform automatic maintenance on the instrument, or give an alarm Function. Enables the user to obtain valid results, or to be informed that the instrument is malfunctioning and needs to be repaired.
- the instrument maintenance includes at least one of the following: the instrument automatically performs reaction cup cleaning, reagent needle cleaning, sample needle cleaning, stirring rod cleaning, or discharges the cleaning agent into the reaction cup or through the cleaning mechanism, cleaning needle, etc. into the cleaning pool for cleaning and other maintenance processes.
- the alarm or prompt of the instrument includes at least one of the following: feedback from the instrument according to the signal, abnormal alarm test results, abnormal operation of the optical signal acquisition component, abnormal operation of the light source lamp, abnormal movement state of the reaction plate, abnormal operation of the cuvette cleaning mechanism, etc. , or the alarm requires manual instrument maintenance.
- the sample analyzer provided in the embodiment of the present application includes a sampling device, a reaction device, and a detection device.
- the detection device includes a light source, an optical signal acquisition component, a memory, and a processor; the sampling device is used to suck a sample of a predetermined volume and transport the sample to a cuvette; The reagents and samples are mixed in the reaction cup to prepare the reaction solution; the reaction cup rotates relative to the detection device, and during the rotation movement relative to the detection device, the light source irradiates the reaction cup with the light from the light source, and the light source moves relative to a reaction cup and continues to irradiate
- the process constitutes a detection cycle, the optical signal acquisition component collects multiple signals of the cuvette in a detection cycle, the memory stores the intensity of the collected signals and the time of signal collection, and obtains the intensity and The signal sequence of the time correspondence relationship, the processor processes the signal sequence. By collecting and storing the intensity and time of multiple signals within a cuvette detection cycle to form a signal
- the exit light at the same position of the cuvette is divided into optical signals of at least two wavelengths by a spectroscopic device, and at least two optical signals are collected and processed at least two optical signals to obtain at least two photoelectric data, which can be simultaneously obtained
- the photoelectric data of multiple wavelengths at the same position in the same time period in order to obtain more accurate processing results.
- Step S110 during the relative movement of the cuvette and the detector in the sample analyzer, collect the intensity and time information of multiple first signals of the first cuvette in the movement process, optionally, the cuvette in the cuvette Contains the reaction solution obtained by reacting the sample and the reagent;
- Step S120 storing the first signal to obtain a first signal sequence, where the first signal sequence includes a corresponding relationship between the intensity and time of the first signal.
- the first cuvette and the second cuvette are adjacent to each other and pass through the detector successively; or the first cuvette and the second cuvette are the same cuvette, and are detected successively after a plurality of cuvettes are separated. device.
- control method of the sample analyzer includes the following steps S210 to S230.
- Step S220 collecting at least two optical signals at the same time, the at least two optical signals are formed by the irradiation light irradiating the cuvette or the cuvette liquid, and the wavelengths of at least two of the optical signals are different;
- Step S240 performing state monitoring according to at least two of the photoelectric data, wherein the state monitoring at least includes performing state monitoring on the liquid in the cuvette, the cuvette or the detection device of the sample analyzer at least one of the .
- the cuvette rotates around the light source assembly;
- the photoelectric data is a signal sequence formed by the relationship between signal intensity and collection time, which is collected during the continuous illumination of the cuvette by the light source assembly
- the photoelectric data includes signal sequences corresponding to at least two wavelengths, and the signal sequence of the same wavelength includes a corresponding relationship between the intensity and time of at least two of the signals.
- the cuvette rotates relative to the light source assembly, and the light source assembly is a light source array, and the cuvette sequentially passes through each point light source in the light source array, and each point light source has a different wavelength.
- State monitoring is performed according to the photoelectric data corresponding to the target wavelength.
- the state monitoring based on at least two photoelectric data includes:
- the photoelectric data corresponding to each wavelength is abnormal, it is determined that the cuvette is fouled, there is foreign matter in the cuvette, there is foreign matter in the liquid in the cuvette, or the detection device of the sample analyzer is abnormal.
- the state monitoring based on at least two photoelectric data includes:
- the state monitoring based on at least two photoelectric data includes:
- Condition monitoring is carried out according to the abnormal photoelectric data.
- the light intensity value includes the peak value of the photoelectric data
- the cuvette and the light source assembly move relatively
- the photoelectric data is a signal sequence formed by the relationship between signal intensity and acquisition time.
- the photoelectric data packets collected during the continuous irradiation of the same cuvette by the light source assembly correspond to at least two signal sequences of wavelengths, and the signal sequences of the same wavelength include the corresponding relationship between the intensity and time of at least two of the signals.
- photoelectric data with multiple wavelengths at the same position can be obtained, so as to obtain more accurate processing results.
- FIG. 24 is a schematic flowchart of a control method of a sample analyzer provided by an embodiment of the present application.
- control method of the sample analyzer includes the following steps S310 to S340.
- Step S310 controlling the light source assembly of the sample analyzer to generate irradiating light, and the irradiating light irradiates a cuvette or the same position in the cuvette liquid;
- Step S320 collecting at least two optical signals at the same time, the at least two optical signals are formed by the irradiation light irradiating the cuvette or cuvette liquid, and at least two optical signals have different wavelengths;
- Step S330 process at least two of the optical signals to obtain at least two photoelectric data, the photoelectric data packets collected during the process of the light source assembly continuously irradiating the same cuvette are signal sequences corresponding to at least two wavelengths, the same The signal sequence of the wavelength includes the corresponding relationship between the intensity and time of at least two signals;
- Step S340 acquiring the detection result of the liquid in the cuvette according to at least two of the photoelectric data.
- the acquisition of the detection results of the liquid in the cuvette according to at least two of the photoelectric data includes:
- the acquisition of the detection results of the liquid in the cuvette according to at least two of the photoelectric data includes:
- the detection result of the liquid in the cuvette is obtained according to the photoelectric data corresponding to the target wavelength.
- the detection result of the liquid in the cuvette is obtained according to the corrected photoelectric data.
- the step of acquiring the detection result of the sample added in the cuvette according to the photoelectric data of at least two wavelengths includes:
- the detection result of the sample added in the cuvette is obtained according to the reactivity of the liquid in the cuvette.
- control method also includes:
- a reaction curve corresponding to at least two wavelengths corresponding to the added sample in the cuvette is generated, and the response curve is determined by the signal intensity of multiple detection cycles of the cuvette It is obtained that each time the light source assembly continues to irradiate the cuvette forms a detection cycle;
- Response curves of at least two wavelengths are output, wherein the response curve corresponds to a line segment in each detection period.
- the specific principle and implementation of the control method provided in the embodiment of the present application are similar to the sample analyzer in the foregoing embodiment, and will not be repeated here.
- FIG. 25 is a schematic flowchart of a control method of a sample analyzer provided by an embodiment of the present application.
- control method of the sample analyzer includes the following steps S410 to S460.
- Step S410 controlling the light source assembly of the sample analyzer to generate irradiating light, and the irradiating light irradiates a cuvette or the same position in the cuvette liquid;
- Step S420 collecting at least two optical signals at the same time, the at least two optical signals are formed by the irradiation light irradiating the cuvette or the cuvette liquid, and the wavelengths of at least two of the optical signals are different;
- Step S430 processing at least two of the optical signals to obtain at least two photoelectric data
- Step S440 acquiring the first photoelectric data corresponding to the first wavelength and the second photoelectric data of the second wavelength among the at least two photoelectric data;
- Step S450 using the second photoelectric data to correct the first photoelectric data to obtain third photoelectric data
- Step S460 processing the third photoelectric data to obtain a processing result.
- using the second photoelectric data to correct the first photoelectric data to obtain third photoelectric data includes:
- the correlation parameter includes a correlation curve or a correlation coefficient
- the acquiring the first photoelectric data corresponding to the first wavelength and the second photoelectric data of the second wavelength in at least two of the photoelectric data includes:
- the second photoelectric data of the second wavelength is determined according to the target detection item of the sample added in the cuvette.
- the processing result includes a detection result of liquid in the cuvette.
- the cuvette rotates around the light source assembly;
- the photoelectric data is a signal sequence formed by the relationship between signal intensity and collection time, which is collected during the continuous illumination of the cuvette by the light source assembly
- the photoelectric data includes signal sequences corresponding to at least two wavelengths, and the signal sequence of the same wavelength includes a corresponding relationship between the intensity and time of at least two of the signals.
- the functional modules/units in the system, and the device can be implemented as software, firmware, hardware, and an appropriate combination thereof.
- the division between functional modules/units mentioned in the above description does not necessarily correspond to the division of physical components; for example, one physical component may have multiple functions, or one function or step may be composed of several physical components. Components cooperate to execute.
- Some or all of the physical components may be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit .
- Such software may be distributed on computer readable media, which may include computer storage media (or non-transitory media) and communication media (or transitory media).
- computer storage media includes both volatile and nonvolatile media implemented in any method or technology for storage of information, such as computer readable instructions, data structures, program modules, or other data. permanent, removable and non-removable media.
- Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technology, CD-ROM, digital versatile disk (DVD) or other optical disk storage, magnetic cartridges, tape, magnetic disk storage or other magnetic storage devices, or can Any other medium used to store desired information and which can be accessed by a computer.
- communication media typically embodies computer readable instructions, data structures, program modules, or other data in a modulated data signal such as a carrier wave or other transport mechanism, and may include any information delivery media .
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Abstract
Description
Claims (25)
- 一种样本分析仪的控制方法,其特征在于,包括:控制样本分析仪的光源组件产生照射光,所述照射光照射一反应杯或者一反应杯液体中的相同位置;采集至少两个光信号,所述至少两个光信号由所述照射光照射所述反应杯或者反应杯液体后出射形成,至少两个所述光信号的波长不同;对至少两个所述光信号进行处理得到至少两个光电数据;根据至少两个所述光电数据进行状态监测,其中,所述状态监测至少包括,对所述反应杯中的液体、所述反应杯或所述样本分析仪的检测装置进行状态监测中的至少一个。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述反应杯围绕所述光源组件转动;所述光电数据为信号的强度与采集时间之间的关系形成的信号序列,在所述光源组件持续照射所述反应杯的过程中采集的所述光电数据包括至少两个波长对应的信号序列,同一波长的信号序列包括至少两个所述信号的强度和时间的对应关系。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述照射光同时照射一反应杯或者一反应杯液体中的相同位置,且同时采集至少两个所述光信号;或者,所述反应杯相对所述光源组件转动,所述光源组件为光源阵列,反应杯依次经过光源阵列中的各个点光源,各个点光源的波长不同。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据进行状态监测,包括:获取所述光源组件照射的所述反应杯中的液体的目标检测项目或者所述光源组件照射的反应杯中的液体的类型;根据所述目标检测项目或者所述液体的类型确定目标波长;根据所述目标波长对应的所述光电数据进行状态监测。
- 如权利要求4所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据进行状态监测,包括:在所述目标波长对应的光电数据产生异常时,确定所述反应杯中的液体异常。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据进行状态监测,包括:在各个波长对应的所述光电数据均异常时,确定所述反应杯污损、所述反应杯中存在异物、所述反应杯中的液体存在异物或者所述样本分析仪的检测装置异常。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据进行状态监测,包括:确定各个波长对应的所述光电数据是否异常;在所有所述光电数据均异常时,确定所述反应杯污损、所述反应杯中存在异物、所述反应杯中的液体存在异物或者所述样本分析仪的检测装置异常;在部分所述光电数据异常时,确定所述反应杯中的液体异常或者所述反应杯变色。
- 如权利要求1所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据进行状态监测,包括:比较至少两个波长对应的所述光电数据的光强度值;在所述至少两个波长对应的所述光强度值之间的大小关系不符合预设条件时,判定不符合所述预设条件的所述光强度值对应的光电数据中的至少一个异常;或者,在所述至少两个波长对应的所述光强度值之间的差值大于预设阈值时,判定差值大于所述预设阈值的所述光强度值对应的光电数据中的至少一个异常;根据异常的所述光电数据进行状态监测。
- 如权利要求8所述的样本分析仪的控制方法,其特征在于,所述光强度值包括所述光电数据的峰值,所述反应杯与所述光源组件相对移动,所述光电数据为信号的强度与采集时间之间的关系形成的信号序列,在所述光源组件持续照射同一反应杯的过程中采集的所述光电数据包至少两个波长对应的信号序列,同一波长的信号序列包括至少两个所述信号的强度和时间的对应关系。
- 一种样本分析仪的控制方法,其特征在于,包括:控制样本分析仪的光源组件产生照射光,所述照射光照射一反应杯或者反应杯液体中的相同位置;采集至少两个光信号,所述至少两个光信号由所述照射光照射所述反应杯 或者反应杯液体后出射形成,至少两个所述光信号的波长不同;对至少两个所述光信号进行处理得到至少两个光电数据,在所述光源组件持续照射同一反应杯的过程中采集的所述光电数据包至少两个波长对应的信号序列,同一波长的信号序列包括至少两个信号的强度和时间的对应关系;根据至少两个所述光电数据获取反应杯中液体的检测结果。
- 如权利要求10所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据获取反应杯中液体的检测结果,包括:获取所述信号序列的上升沿和下降沿;根据所述上升沿和下降沿在所述信号序列中提取第一信号子序列,并根据至少两个所述第一信号子序列获取反应杯中液体的检测结果,所述第一信号子序列位于所述上升沿和下降沿之间。
- 如权利要求10所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据获取反应杯中液体的检测结果,包括:在所述光源组件照射所述反应杯的过程中产生至少一个参考信号;根据监测到的参考信号在所述反应杯对应的信号序列中提取第二信号子序列;根据所述第二信号子序列获取反应杯中液体的检测结果。
- 如权利要求10所述的样本分析仪的控制方法,其特征在于,所述根据至少两个所述光电数据获取反应杯中液体的检测结果,包括:获取所述光源组件照射的反应杯中添加的样本对应的目标检测项目;根据所述目标检测项目在至少两个所述光电数据中确定目标波长对应的光电数据;根据所述目标波长对应的光电数据获取所述反应杯中液体的检测结果。
- 如权利要求13所述的样本分析仪的控制方法,其特征在于,所述根据所述目标检测项目在至少两个所述光电数据中确定目标波长对应的光电数据之后,还包括:判断所述目标波长对应的光电数据是否异常;在所述目标光电数据未出现异常时,根据所述目标波长对应的光电数据确定所述反应杯中添加的样本的检测结果;在所述目标光电数据出现异常时,采用除目标波长之外的其它波长的光电数据修正所述目标波长的光电数据;根据修正后的所述光电数据获取所述反应杯中液体的检测结果。
- 如权利要求10所述的样本分析仪的控制方法,其特征在于,所述根据至少两个波长的所述光电数据获取反应杯中添加的样本的检测结果的步骤包括:获取至少两个波长的权重,以及根据至少两个波长的光电数据获取至少两个波长中各个波长对应的反应度;根据各个所述波长的权重以及反应度获取所述反应杯中液体的反应度;根据所述反应杯中液体的反应度获取述反应杯中添加的样本的检测结果。
- 如权利要求10所述的样本分析仪的控制方法,其特征在于,还包括:根据同一反应杯对应的至少两个波长对应的光电数据,生成反应杯中的添加的样本对应的至少两个波长对应的反应曲线,所述反应曲线由反应杯的多个检测周期的信号的强度得到,所述光源组件每次持续照射所述反应杯的时长形成一个检测周期;输出至少两个波长的反应曲线,其中,反应曲线在每个检测周期对应一个线段。
- 一种样本分析仪的控制方法,其特征在于,包括:控制样本分析仪的光源组件产生照射光,所述照射光照射一反应杯或者反应杯液体中的相同位置;采集至少两个光信号,所述至少两个光信号由所述照射光照射所述反应杯或者反应杯液体后出射形成,至少两个所述光信号的波长不同;对至少两个所述光信号进行处理得到至少两个光电数据;获取至少两个所述光电数据中第一波长对应的第一光电数据以及第二波长的第二光电数据;采用所述第二光电数据对所述第一光电数据进行修正得到第三光电数据;对所述第三光电数据进行处理得到处理结果。
- 如权利要求17所述的样本分析仪的控制方法,其特征在于,所述采用所述第二光电数据对所述第一光电数据进行修正得到第三光电数据,包括:获取所述第一波长以及所述第二波长之间的相关性参数,所述相关性参数包括相关性曲线或者相关性系数;采用所述相关性参数对所述第一光电数据以及第二光电数据进行拟合得到所述第三光电数据。
- 如权利要求17所述的样本分析仪的控制方法,其特征在于,同时采集 多个所述光信号,所述获取至少两个所述光电数据中第一波长对应的第一光电数据以及第二波长的第二光电数据,包括:获取至少两个光电数据中出现异常的第一光电数据;根据所述反应杯中添加的样本的目标检测项目确定第二波长的第二光电数据。
- 如权利要求17所述样本分析仪的控制方法,其特征在于,所述处理结果包括对所述反应杯中液体的进行检测的检测结果。
- 如权利要求17-20任一项所述样本分析仪的控制方法,其特征在于,所述反应杯围绕所述光源组件转动;所述光电数据为信号的强度与采集时间之间的关系形成的信号序列,在所述光源组件持续照射所述反应杯的过程中采集的所述光电数据包括至少两个波长对应的信号序列,同一波长的信号序列包括至少两个所述信号的强度和时间的对应关系。
- 一种样本分析仪,其特征在于,包括采样装置、试剂分注装置、反应装置、检测装置以及处理器;所述采样装置,用于从被调度到采样位的样本容器中吸取样本,输送吸取的样本到反应杯;所述试剂分注装置,用于将试剂分注至所述反应杯;所述反应装置,用于对反应杯中添加的样本进行孵育,所述反应液由所述试剂和所述样本制备得到;所述检测装置,所述检测装置包括光源组件、光信号采集组件和模数转换组件,所述光源组件用于产生照射光,所述照射光照射一反应杯或者反应杯液体中的相同位置,且所述反应杯相对所述光源组件转动,所述光信号采集组件同时采集至少两个光信号,所述至少两个光信号由所述照射光照射所述反应杯或者反应杯液体后出射形成,至少两个所述光信号的波长不同;所述模数转换组件将至少两个所述光信号模数转换处理得到至少两个光电数据,所述光电数据为信号的强度与采集时间之间的关系形成的信号序列,在所述光源组件持续照射同一反应杯的过程中采集的所述光电数据包括至少两个波长对应的信号序列,同一波长的信号序列包括至少两个所述信号的强度和时间的对应关系;所述处理器用于对至少两个波长的所述光电数据进行处理得到处理结果。
- 如权利要求22所述的样本分析仪,其特征在于,所述样本分析仪还包 括分光装置,所述分光装置将所述反应杯的出射光分为至少两个波长的光信号。
- 如权利要求22所述的样本分析仪,其特征在于,所述样本分析仪还包括分光装置,所述分光装置将所述光源组件产生的照射光分为至少两个波长的照射光,所述至少两个波长的照射光同时照射一反应杯或者反应杯液体中的相同位置;或者,所述光源组件包括至少两个光源,至少两个光源的照射光地波长不同,且同时照射一反应杯或者反应杯液体中的相同位置;或者,所述光源组件包括光源阵列,所述光源阵列中的至少两个光源产生的照射光的波长不同,反应杯相对所述光源转动,至少两个光源沿反应杯地转动方向排布,以使所述反应杯依次经过所述光源阵列中的各个所述光源。
- 如权利要求22-24中任一项所述的样本分析仪,所述处理器还用于:根据至少两个波长的所述光电数据对所述样本分析仪进行状态监测;或者,根据至少两个波长的所述光电数据获取所述反应杯中液体的检测结果。
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