WO2022270037A1 - Dispositif de photométrie et dispositif d'analyse - Google Patents

Dispositif de photométrie et dispositif d'analyse Download PDF

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Publication number
WO2022270037A1
WO2022270037A1 PCT/JP2022/010527 JP2022010527W WO2022270037A1 WO 2022270037 A1 WO2022270037 A1 WO 2022270037A1 JP 2022010527 W JP2022010527 W JP 2022010527W WO 2022270037 A1 WO2022270037 A1 WO 2022270037A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
cuvette
photometric
base member
optical axis
Prior art date
Application number
PCT/JP2022/010527
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English (en)
Japanese (ja)
Inventor
堅志 米山
Original Assignee
古野電気株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 古野電気株式会社 filed Critical 古野電気株式会社
Priority to CN202280037554.1A priority Critical patent/CN117396748A/zh
Priority to JP2023529545A priority patent/JPWO2022270037A1/ja
Publication of WO2022270037A1 publication Critical patent/WO2022270037A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid

Definitions

  • the present invention mainly relates to an analyzer that analyzes a reaction liquid between a specimen and a reagent.
  • an analyzer that analyzes the components of a specimen by reacting the specimen with a reagent.
  • a plurality of cuvettes containing specimens and reagents are arranged in a circle on a cuvette table, the cuvette table is rotated, and a photometric position between a light source and a spectroscopic detector placed across the cuvette is detected. to pass through the cuvette.
  • the component of the specimen is analyzed by measuring the absorbance from the amount of light transmitted through the cuvette.
  • Patent Document 1 describes a reaction disk (cuvette table) that holds reaction containers (cuvettes) on the circumference and repeats rotation and stop, and a reaction container that is placed at a photometric position and contains a mixture of a sample and a reagent.
  • a detector that detects scattered light or transmitted light from the mixed liquid
  • the detector is in the moving direction of the reaction vessel due to the rotation of the reaction disk
  • One or more sets are arranged symmetrically at equal angles or equal intervals around the optical axis of the light emitted from the light source in a plane perpendicular to the light source, and the averaged value and/or sum of the light intensity data from each detector is used to calculate the concentration of the substance to be measured in the mixed liquid. That is, in Patent Document 1, scattered light is received by light receiving elements linearly arranged in the vertical direction.
  • the intensity of scattered light from particles is much smaller than that of transmitted light.
  • fluctuations Brown motion
  • this fluctuation causes the distribution of the amount of scattered light to vary greatly depending on the radiation direction.
  • Patent Document 1 since the light-receiving elements that receive the scattered light are linearly arranged, there is a problem that the intensity of the scattered light cannot be accurately measured by photometry in a short time (for example, 100 us).
  • the present invention has been made to solve the above problems, and an object of the present invention is to provide a photometric device capable of accurately measuring the intensity of scattered light in a short period of time.
  • a photometric device includes a light source for irradiating an irradiated area of a cuvette placed at a photometric position, a base member facing the light source, and light emitted from the cuvette at the photometric position on the base member.
  • a plurality of first light-receiving elements arranged in a planar region defined by a first radiation direction and a second radiation direction centering on the optical axis of the transmitted light, and receiving scattered light scattered from the cuvette.
  • FIG. 4 is a schematic diagram showing the positional relationship between the cuvette and the base member; 4 is a graph showing the relationship between scattered light intensity (scattered light amount) and particle size.
  • A) is a perspective view of a filter portion in a first mode
  • B) is a perspective view of a filter portion in a second mode.
  • FIG. 1 is a plan view showing a schematic configuration of an analysis device 1 according to one embodiment of the present invention.
  • An analyzer 1 is an apparatus for analyzing a sample (for example, blood, urine, etc.) and a reaction liquid of a reagent, and includes a cuvette table 3 on which rows of cuvettes 2 are arranged in a circle, a driving unit 4, and a photometric device 5. , an optical sensor 6 and an analysis unit 7 .
  • the cuvette table 3 is formed in an annular shape in plan view, and a plurality of cuvettes 2 are arranged along the annular direction (arc-shaped arrow line in FIG. 1).
  • the cuvette 2 is a container for containing a specimen and a reagent, and has a cubic or rectangular parallelepiped shape with an open top.
  • a sample storage (not shown) that stores sample containers
  • a reagent store (not shown) that stores reagent containers, and the like.
  • a pipette (not shown) is used to supply the sample and reagent from the sample container and the reagent container to the cuvette 2 .
  • the drive unit 4 is a member that rotates the row of cuvettes 2 in an annular direction.
  • the driving section 4 comprises a driving gear 41 and a driven gear 42 connected to the cuvette table 3 .
  • the driving gear 41 is attached to a stepping motor (not shown), and by driving the stepping motor to rotate the driving gear 41 , the cuvette table 3 can be rotated via the driven gear 42 .
  • the mechanism for rotating the cuvette table 3 is not limited to this.
  • a pulley may be attached to the center shaft of the cuvette table 3 and driven by a timing belt.
  • the photometry device 5 is a member that irradiates light onto each cuvette 2 passing through the photometry position P while the row of cuvettes 2 rotates, and measures the emitted light from the irradiated region of the cuvette 2 at the photometry position P.
  • FIG. 1 shows a light source 51 , a base member 52 and a first light receiving element 53 as members constituting the photometric device 5 . A more detailed configuration of the photometric device 5 will be described later.
  • the cuvette table 3 is provided with slits 31 arranged corresponding to each cuvette 2 .
  • the number of slits 31 is the same as the number of cuvettes 2 , and the slits 31 are arranged in an annular direction on the outer peripheral edge of the cuvette table 3 .
  • the optical sensor 6 is a member that detects the slit 31 .
  • the optical sensor 6 has a U-shape and includes a light source 61 and a light receiving element 62 facing each other with the outer peripheral edge of the cuvette table 3 interposed therebetween.
  • the emitted light from the light source 61 reaches the light receiving element 62 only while the slit 31 is passing between the light source 61 and the light receiving element 62, and the voltage signal photoelectrically converted by the light receiving element 62 is output to the analysis unit 7. be done.
  • FIG. 3 is a perspective view showing the detailed configuration of the photometric device 5.
  • the photometric device 5 mainly includes a light source 51 , a base member 52 , a plurality of first light receiving elements 53 , a mirror 54 , a spectral mirror 55 , a plurality of second light receiving elements 56 and a filter section 57 . ing.
  • the light source 51 irradiates the irradiated area of each cuvette 2 placed at the photometric position P with light.
  • the light incident on the irradiated region of the cuvette 2 passes through the interior of the cuvette 2 and exits from the irradiated region of the cuvette 2 (more precisely, the back surface of the irradiated region).
  • a halogen lamp that emits light of multiple wavelengths (for example, 380 nm to 800 nm) in a predetermined wavelength band can be used.
  • the base member 52 is an annular flat plate facing the light source 51 .
  • the base member 52 has a circular opening 52a in its center, and transmitted light emitted from the cuvette 2 passes through the opening 52a.
  • the first light receiving element 53 is arranged on the side facing the photometry position P with respect to the base member 52 .
  • the first light receiving element 53 is arranged on the base member 52 in a first radiation direction D1 and a second radiation direction D2 centered on the optical axis La of the transmitted light emitted from the cuvette 2 at the photometry position P. and receive scattered light scattered from the cuvette 2 at the photometric position P, respectively.
  • the planar region where the first light receiving element 53 is arranged is an annular region surrounding the optical axis La on the base member 52, and is based on the scattering range due to fluctuation of particles in the reaction liquid of the cuvette 2.
  • the first light receiving elements 53 are planarly arranged in an annular region.
  • the first radiation direction D1 is the direction from the photometry position P to the inner edge of the base member 52 (the edge of the opening 52a), and the second radiation direction D2 is the direction from the photometry position P to the outer edge of the base member 52. be.
  • the photometry position P is indicated by a dot in FIG. 4, it is not particularly limited as long as it is within the reaction liquid inside the cuvette 2 on the optical axis La.
  • the first radiation direction D1 may be a direction from the photometry position P toward the outside of the inner edge of the base member 52
  • the second radiation direction D2 may be a direction from the photometry position P toward the inside of the outer edge of the base member 52. may be
  • the scattered light intensity I( ⁇ ) is inversely proportional to the scattering angle ⁇ .
  • the scattering angle ⁇ is the angle between the direction of emission of the scattered light and the optical axis La.
  • FIG. 5 is a graph showing the relationship between scattered light intensity (scattered light amount) and particle size. It can be seen that the scattered light intensity increases mainly when the scattering angle is 20° to 30°. Therefore, in this embodiment, as shown in FIG. 4, the angle ⁇ 1 formed between the first radiation direction D1 and the optical axis La is set to 20°, and the angle ⁇ 2 formed between the second radiation direction D2 and the optical axis La is set to 30°. ° is set.
  • the angle between the straight line connecting the first light receiving element 53 and the photometry position P and the optical axis La is 20° to 30°, and most of the scattered light emitted from the cuvette 2 is incident on
  • Each first light-receiving element 53 photoelectrically converts the scattered light and outputs a voltage signal having an intensity corresponding to the amount of light to the analysis unit 7 .
  • the analysis unit 7 calculates the total value of the voltage signals from the respective first light receiving elements 53 as the intensity of the scattered light.
  • the distribution of the amount of scattered light varies greatly depending on the radiation direction due to Brownian motion of particles. That is, scattered light is not emitted uniformly in a radial pattern.
  • the plurality of first light receiving elements 53 are arranged in a plane, even if the amount of scattered light varies locally, the entire area where the first light receiving elements 53 are arranged is almost constant. Therefore, the scattered light intensity can be accurately measured in a short time by correcting variations in the amount of scattered light.
  • the values of the angles ⁇ 1 and ⁇ 2 are not limited to the above values, and can be changed as appropriate according to the particle size of the particles.
  • the base member 52 is movable along the optical axis La by a drive mechanism (not shown), and by moving the base member 52, the angles ⁇ 1 and ⁇ 2 can be adjusted.
  • the transmitted light that has passed through the opening 52a is incident on the spectroscopic mirror 55 via the mirror 54, and the spectroscopic mirror 55 disperses the incident light by wavelength band and reflects it to the second light receiving element 56.
  • Each of the second light receiving elements 56 photoelectrically converts the transmitted light in different wavelength bands, and outputs a voltage signal having an intensity corresponding to the amount of light to the analysis section 7 .
  • the second light receiving element 56 may be provided near the center of the base member 52 without providing the opening 52a in the base member 52 .
  • disposing the second light receiving element 56 on the opposite side of the base member 52 to the photometric position P as in the present embodiment facilitates the installation of an optical system that disperses the transmitted light.
  • the filter section 57 is connected to a rotating shaft 58a rotated by a motor 58.
  • the filter section 57 includes a cylindrical section 573 and single-wavelength filters 574 provided inside both ends of the cylindrical section 573 .
  • Two circular passage holes 57a are formed in opposing side surfaces of the cylindrical portion 573 in the middle portion in the longitudinal direction.
  • the central axis of the cylindrical portion 573, the line connecting the centers of the two through holes 57a, and the longitudinal direction of the rotating shaft 58a are orthogonal to each other.
  • the filter part 57 is oriented so that the central axis of the cylindrical part 573 coincides with the emission direction of the light L1 from the light source 51 .
  • the multi-wavelength light L1 passes through the single-wavelength filter 574, and the single-wavelength filter 574 passes the single-wavelength (for example, 700 nm) light L2.
  • the light L2 is scattered at the photometry position P, emitted from each cuvette 2, and received by the first light receiving element 53.
  • the filter part 57 is oriented so that the line connecting the centers of the two passage holes 57a coincides with the emission direction of the light L1.
  • the multi-wavelength light L1 in the predetermined wavelength band passes through the passage hole 57a as it is.
  • the light L1 passes through the photometric position P, is emitted from each cuvette 2, and is received by the second light receiving element 56.
  • both the transmitted light and the scattered light of the reaction liquid can be photometrically measured.
  • the first light receiving element 53 is arranged in a ring-shaped region on the base member 52 in a planar manner, but the arrangement is not limited to a circular ring as long as it is arranged in a planar manner.
  • the first light receiving elements 53 can be arranged in a rectangular frame shape or an arc shape.
  • the planar region in which the first light receiving element 53 is arranged may be a discontinuous surface.
  • the photometric device 5 can measure both the transmitted light and the scattered light of the reaction liquid, it may be possible to measure only the scattered light.
  • the light source 51 a light source that emits light of a single wavelength, such as a semiconductor laser, can be used, and the filter section 57 need not be provided.
  • the photometric device 5 has the base member 52 disposed on the opposite side of the photometric position P from the light source 51 (that is, disposed between the photometric position P and the mirror 54). ), and the plurality of first light receiving elements 53 are arranged on the side facing the photometric position P with respect to the base member 52 , but the present invention is not limited to this.
  • the base member 52 may be arranged between the photometry position P and the light source 51 (or the filter section 57), and the plurality of first light receiving elements 53 may be arranged on the side facing the photometry position P with respect to the base member 52. .

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Le problème décrit par la présente invention est de fournir un dispositif de photométrie qui peut mesurer une intensité de lumière diffusée précise en un court laps de temps. La solution selon l'invention porte sur un dispositif de photométrie 5 qui comprend : une source de lumière 51 qui rayonne de la lumière sur une zone d'irradiation d'une cuvette 2 disposée dans une position photométrique P ; un élément de base 52 à l'opposé de la source de lumière 51 ; et une pluralité de premiers éléments de réception de lumière 53 qui sont disposés, sur l'élément de base 52, à l'intérieur d'une région plane délimitée par une première direction de rayonnement et une seconde direction de rayonnement centrée autour de l'axe optique de la lumière transmise émise à partir de la cuvette 2 dans la position photométrique P, et reçoivent respectivement la lumière diffusée diffusée par la cuvette 2 dans la position photométrique P.
PCT/JP2022/010527 2021-06-25 2022-03-10 Dispositif de photométrie et dispositif d'analyse WO2022270037A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202280037554.1A CN117396748A (zh) 2021-06-25 2022-03-10 测光装置和分析装置
JP2023529545A JPWO2022270037A1 (fr) 2021-06-25 2022-03-10

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021105427 2021-06-25
JP2021-105427 2021-06-25

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Publication Number Publication Date
WO2022270037A1 true WO2022270037A1 (fr) 2022-12-29

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006329629A (ja) * 2005-05-23 2006-12-07 Yokogawa Electric Corp 濁度計
JP2008058155A (ja) * 2006-08-31 2008-03-13 Hitachi High-Tech Science Systems Corp 医用光度計
JP2009014602A (ja) * 2007-07-06 2009-01-22 Toshiba Corp 自動分析装置
WO2011093402A1 (fr) * 2010-01-29 2011-08-04 株式会社日立ハイテクノロジーズ Dispositif d'analyse
WO2011105464A1 (fr) * 2010-02-25 2011-09-01 株式会社日立ハイテクノロジーズ Dispositif d'analyse automatique
JP2012149903A (ja) * 2011-01-17 2012-08-09 Hitachi High-Technologies Corp 自動分析装置
JP2013036807A (ja) * 2011-08-05 2013-02-21 Dkk Toa Corp 濁度計
JP2020024125A (ja) * 2018-08-07 2020-02-13 キヤノン株式会社 自動分析装置、自動分析方法、および、プログラム

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006329629A (ja) * 2005-05-23 2006-12-07 Yokogawa Electric Corp 濁度計
JP2008058155A (ja) * 2006-08-31 2008-03-13 Hitachi High-Tech Science Systems Corp 医用光度計
JP2009014602A (ja) * 2007-07-06 2009-01-22 Toshiba Corp 自動分析装置
WO2011093402A1 (fr) * 2010-01-29 2011-08-04 株式会社日立ハイテクノロジーズ Dispositif d'analyse
WO2011105464A1 (fr) * 2010-02-25 2011-09-01 株式会社日立ハイテクノロジーズ Dispositif d'analyse automatique
JP2012149903A (ja) * 2011-01-17 2012-08-09 Hitachi High-Technologies Corp 自動分析装置
JP2013036807A (ja) * 2011-08-05 2013-02-21 Dkk Toa Corp 濁度計
JP2020024125A (ja) * 2018-08-07 2020-02-13 キヤノン株式会社 自動分析装置、自動分析方法、および、プログラム

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CN117396748A (zh) 2024-01-12
JPWO2022270037A1 (fr) 2022-12-29

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