WO2022250074A1 - Transformant d'organisme photosynthétique et son utilisation - Google Patents

Transformant d'organisme photosynthétique et son utilisation Download PDF

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Publication number
WO2022250074A1
WO2022250074A1 PCT/JP2022/021364 JP2022021364W WO2022250074A1 WO 2022250074 A1 WO2022250074 A1 WO 2022250074A1 JP 2022021364 W JP2022021364 W JP 2022021364W WO 2022250074 A1 WO2022250074 A1 WO 2022250074A1
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WO
WIPO (PCT)
Prior art keywords
proton pump
transformant
rhodopsin
cell
photosynthetic organism
Prior art date
Application number
PCT/JP2022/021364
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English (en)
Japanese (ja)
Inventor
雄気 須藤
慧一 小島
Original Assignee
国立大学法人 岡山大学
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Publication date
Application filed by 国立大学法人 岡山大学 filed Critical 国立大学法人 岡山大学
Priority to JP2023523500A priority Critical patent/JPWO2022250074A1/ja
Publication of WO2022250074A1 publication Critical patent/WO2022250074A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

Le but de la présente invention est de procurer un nouveau procédé pouvant réguler la croissance d'un organisme photosynthétique et de fournir une utilisation de ce procédé. La solution selon l'invention porte sur un transformant d'organisme photosynthétique exprimant une rhodopsine à pompe à protons, et sur un procédé de régulation de la croissance d'un organisme photosynthétique, le procédé comprenant les étapes suivantes : (1) obtention d'un transformant exprimant une rhodopsine à pompe à protons ; et (2) entraînement de la rhodopsine à pompe à protons par exposition du transformant à la lumière.
PCT/JP2022/021364 2021-05-28 2022-05-25 Transformant d'organisme photosynthétique et son utilisation WO2022250074A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023523500A JPWO2022250074A1 (fr) 2021-05-28 2022-05-25

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021-089800 2021-05-28
JP2021089800 2021-05-28

Publications (1)

Publication Number Publication Date
WO2022250074A1 true WO2022250074A1 (fr) 2022-12-01

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PCT/JP2022/021364 WO2022250074A1 (fr) 2021-05-28 2022-05-25 Transformant d'organisme photosynthétique et son utilisation

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JP (1) JPWO2022250074A1 (fr)
WO (1) WO2022250074A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011516029A (ja) * 2007-11-10 2011-05-26 ジュール アンリミテッド インコーポレイテッド 高光合成生物

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011516029A (ja) * 2007-11-10 2011-05-26 ジュール アンリミテッド インコーポレイテッド 高光合成生物

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KANDORI, HIDEKI: "Mechanism of Rhodopsin Function", JOURNAL OF JAPANESE BIOCHEMICAL SOCIETY, NIPPON SEIKAGAKKAI, TOKYO, JP, vol. 91, no. 4, 1 January 2019 (2019-01-01), JP , pages 472 - 481, XP009541352, ISSN: 0037-1017 *
NAGASE, YURIE; INOUE, SAKI; KURODA, HIROSHI; KOJIMA, KEIICHI; YOSHIZAWA, SUSUMU; TAKAHASHI, YUICHIRO; SUDO, YUKI: "3Pos044: Expression of proton pump rhodopsins in the chloroplast of the alga Chlamydomonas reinhardtii for optical control of proton gradient", SEIBUTSU BUTSURI, BIOPHYSICAL SOCIETY OF JAPAN, TOKYO, JP, vol. 58, no. Suppl. 1-2, 1 January 2018 (2018-01-01), JP , pages S412, XP009541369, ISSN: 0582-4052 *
NAGASE, YURIE; KOJIMA, KEIICHI; INOUE, SAKI; KURODA, HIROSHI; TOKUTSU, RYUTARO; MASUDA, SHINJI; MINAGAWA, JUN; TAKAHASHI, YUICHIRO: "1Pos234: Optical control of non-photochemical quenching (NPQ) in the alga Chlamydomonas reinhardtii by light-driven proton pump rhodopsins", SEIBUTSU BUTSURI, BIOPHYSICAL SOCIETY OF JAPAN, TOKYO, JP, vol. 59, no. Suppl. 1-2, 1 January 2019 (2019-01-01), JP , pages S293, XP009541368, ISSN: 0582-4052 *

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JPWO2022250074A1 (fr) 2022-12-01

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