WO2022245243A1 - Composition détergente à base de lipase et de bêta-cyclodextrine - Google Patents

Composition détergente à base de lipase et de bêta-cyclodextrine Download PDF

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Publication number
WO2022245243A1
WO2022245243A1 PCT/RU2021/000271 RU2021000271W WO2022245243A1 WO 2022245243 A1 WO2022245243 A1 WO 2022245243A1 RU 2021000271 W RU2021000271 W RU 2021000271W WO 2022245243 A1 WO2022245243 A1 WO 2022245243A1
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Prior art keywords
composition
lipase
evity
lipex
composition according
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PCT/RU2021/000271
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English (en)
Russian (ru)
Inventor
Елена Юрьевна БЕЛОУС
Виктор Андреевич ФИЛАТОВ
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"Скайлаб At"
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Priority claimed from RU2021110850A external-priority patent/RU2811018C2/ru
Application filed by "Скайлаб At" filed Critical "Скайлаб At"
Priority to EP21940960.4A priority Critical patent/EP4342968A1/fr
Publication of WO2022245243A1 publication Critical patent/WO2022245243A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/20—Organic compounds containing oxygen
    • C11D3/22—Carbohydrates or derivatives thereof
    • C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin

Definitions

  • the invention relates to a composition and its use in a household chemical product, where the composition consists of lipase and b-cyclodextrin, and the mass ratio of lipase and b-cyclodextrin is (0.0025-0.25): (0.1-1) respectively.
  • EFFECT invention provides effective regulation of lipid enzymatic cleavage kinetics with simultaneous effective neutralization of unpleasant odors on various types of surfaces, including especially metal, polymer, enameled, glass and wood, while maintaining long-term cleanliness and pleasant aroma.
  • the main stimulating factors are a decrease in physical effort to remove contamination by hand (more than 33% of responses), a decrease in water consumption for washing off contamination (more than 28% of responses) and a high confirmed effectiveness of the product (more than 27% of responses).
  • the need has been identified for the development of innovative household chemicals that can effectively remove household pollution, reduce the effort spent on cleaning surfaces and are safe for nature and humans.
  • 2% sodium lauryl sulfate, known as SLS, in the composition of household chemicals can cause a loss of transepidermal moisture by 68.9 g/m2/h relative to normal level 12 hours after contact with the agent [Loffler, H., & Happle, R. (2003). Profile of irritant patch testing with detergents: sodium lauryl sulfate, sodium laureth sulfate and alkyl polyglucoside. Contact Dermatitis, 48(1), 26-32]. Since the content of sodium lauryl sulfate in household chemicals can reach up to 29% due to the high washing power, this can adversely affect the condition of the skin of the hands.
  • alkyl sulfates cause denaturation of keratin by destroying sulfide bonds and forming -SH sulfhydryl groups on human cells and washing out the epidermal barrier [Prottey C, Ferguson T. Factors which determine the skin irritation potential of soaps and detergents. J Soc Cosmetic Chem. 1975;26: 29-46.], while alkyl sulfates with a chain length of C 12-C 14, which include SLS, have the maximum activity.
  • Lipase or triacylglycerol-acyl-gyrolase is a water-soluble enzyme from the class of hydrolases that catalyzes the hydrolysis of ester bonds into triglycerides of fatty acids, which are water-insoluble esters of glycerol and higher carboxylic acids of various structures [European Commission Cosmetic Ingredients & Substances Database: http://ec.europa.eu/growth/tools-databases/cosing/].
  • Lipase is registered as a dietary supplement E1104 and can be used to digest, dissolve and fractionate fats.
  • Lipase is a catalyst for the fat splitting reaction and is not a product/starting material in the hydrolysis reaction, which improves the kinetics of the enzymatic reaction. It is known that one enzyme molecule can catalyze up to 10,000 reactions per second, depending on domain organization, functional activity, and raw materials. A small amount of enzyme is sufficient for effective action, since the activity of commercially available lipases is above 40 U/g or U/ml. Thus, the effective concentration of lipase is 0.4-0.8% (w/w) as part of complex additives for implementation in household chemicals [https://www.enzymeinnovation.com/lipase-detergent-everything-you-need- know/].
  • lipases are one of the most effective enzymes for removing greasy stains and lipid contaminants based on vegetable oils (sunflower, olive, rapeseed, corn, linseed, etc.) with different fatty acid composition, animal fats (butter, lard, beef fat, mutton fat, etc.), having a solid state of aggregation under normal conditions, lubricants based on ester components and fatty phases of perfumery and cosmetic products containing oils, waxes, emulsifiers with ester bonds, such as lecithin, perfume components and essential oils.
  • vegetable oils unsunflower, olive, rapeseed, corn, linseed, etc.
  • animal fats butter, lard, beef fat, mutton fat, etc.
  • lubricants based on ester components and fatty phases of perfumery and cosmetic products containing oils, waxes, emulsifiers with ester bonds, such as lecithin, perfume components and essential oils.
  • Lipases are highly active against lipid contaminants on various surfaces, including metal, polymer, wood, ceramic, and do not disrupt the structure of these surfaces, which indicates a gentle effect in the composition of detergents.
  • the most desirable pH optimum is between 5.0 and 11.0, and the activity can be maintained at various temperatures from 0°C to 60°C, in particular the temperature optimum is from 20°C to 40°C.
  • Lipases can be stable in the presence of proteases, chelating agents, peroxide compounds (hydrogen peroxide, sodium percarbonate, etc.) and surfactants, in particular anionic ones.
  • Lipase stabilizers in particular glycerin, propylene glycols, sorbitol, sugars, carboxylic acids, alkylamines, inorganic salts, nonionic and ionic surfactants, as well as a reduced water content in the formulation, can be introduced into the systems, which makes it possible to make the detergent concentrated and with low water consumption in the production of detergents.
  • the use of lipase allows the production of energy-efficient products that save electricity and water, thereby responsibly using resources.
  • the concentration of lipase in household chemicals depends on the activity and can range from 0.0025 to 1% (by weight, in pure form). It was found that the addition of a lipase with an activity of 100 KLU3/g to surfactants in the composition of the laundry detergent showed high efficiency in removing grease stains with a standard wash of 40*C. The effective concentration of lipase (as part of complex additives) with this activity is 0.2-0.6% [N. Uhlig, E. M. Linsmaier-Bednar.
  • Lipase is noted to be a necessary component in detergents for the effective removal of lipid stains and grease. It is the enzymatic activity per unit volume that determines the working concentration of the component in the formulations of detergents for various purposes. With a temporary activity of 50 U/mL and in an amount of 10 mL, the total enzyme activity will be 500 U. With an average enzyme activity of 100-1000 U/g, the effective concentration of lipase (as part of a complex supplement) will be 0.05-5.00 % in detergent formulation [Mamta Chauhan, Rajinder Singh Chauhan, and Vijay Kumar Garlapati. Evaluation of a New Lipase from Staphylococcus sp. for Detergent Additive Capability.
  • lipase is a necessary component in detergents to effectively remove lipid stains and grease by targeting fatty acid triglyceride molecules and improving the effectiveness of detergent systems based on surfactants, in particular nonionic surfactants.
  • surfactants in particular nonionic surfactants.
  • the effective concentration of lipase in its pure form is 0.0025-0.1% in cleaners and detergents for cleaning various surfaces.
  • lipase has a specific odor. Lipase breaks down fats into glycerol and fatty acids. If, for example, these fatty acids are not completely removed from textiles during the washing process, consumers notice a rancid odor that becomes more intense depending on the amount of lipase. (Averyanova V. A. Evolution of washing properties: striving for a sustainable ideal / Raw materials and packaging. 02 (151), 2014). Thus, lipase can lead to irritation of the mucous membranes of the upper respiratory tract and lungs and lead to an allergic reaction. The inventors have successfully overcome this limitation by creating an effective combination.
  • Another functional biodegradable component are cyclodextrins.
  • Smell plays an important role in human life, as it allows you to recognize various compounds and navigate in space, as well as protect yourself from toxic and allergenic substances. It is not always possible to neutralize the unpleasant smell in the room, which causes a feeling of discomfort and does not create a sense of security for a person. Not all household cleaning products have the ability to neutralize unpleasant odors after a thorough cleaning of the home, so there is a need to manage odors to create a comfortable environment.
  • Cyclodextrins are substances of natural origin, cyclic glucose oligomers obtained enzymatically from starch [Crini G. A history of cyclodextrins //Chemical reviews. -2014. - T. 114. - 21. - S. 10940-10975].
  • the o-dextrin cycle contains at least six D-(+) glucopyranose residues linked by a-(1,4)-glucosidic bonds. Glucopyranose residues form an energetically favorable "chair” conformation, which is why cyclodextrin molecules have the shape of cones.
  • Natural cyclodextrins are of three types: a, b and g, corresponding to 6, 7 and 8 glucopyranose residues [B. Bonthagarala, CH BABU RAO, N. Sreekanth. The cyclodextrins: A review// IJPRBS - 2013. - T. 2. - S. 291 - 304].
  • Each of the three types of cycle o dextrins has its own physical and chemical properties, in particular, molecular weight, central cavity diameter, approximate volume cavities and solubility in water, the number of water molecules contained in the cavity of cyclodextrins and pKa of the substance in the aquatic environment.
  • b-cyclodextrin molecules have the largest internal diameter of the cavity, which allows them to form inclusion complexes (“host-guest” complexes) with a wide range of solid, liquid and gaseous compounds through molecular complexation.
  • the guest molecule is retained in the cavity of the cyclodextrin host molecule due to hydrophobic and van der Waals interactions.
  • the hydrophobic cavity of cyclodextrin molecules provides a microenvironment in which non-polar fragments of suitable size can be introduced to form an inclusion complex
  • non-polar fragments of suitable size can be introduced to form an inclusion complex
  • Drug solubilization and stabilization // Journal of pharmaceutical sciences. - 1996. - T. 85. - N°. 10. - C. 1017-1025].
  • the main driving force of complex formation is the release of water molecules from the cavity of cyclodextrins rich in DH enthalpy.
  • the complexes can form either in solution or in the crystalline state, with water usually being the preferred solvent [Hadaruga NG et al. A review on thermal analyzes of cyclodextrins and cyclodextrin complexes //Environmental Chemistry Letters. - 2019. -T. 17.-l. - C. 349-373].
  • the list of potential guests for molecular encapsulation in the o ring of dextrins is quite diverse and includes compounds such as straight or branched chain aliphatic compounds, aldehydes, ketones, alcohols, organic acids, fatty acids, aromatics, gases, and polar compounds such as halogens, hydroxy acids and amines [Del Valle E. M. M. Cyclodextrins and their uses: a review
  • a-cyclodextrins For many encapsulated substances, the cavity size of a-cyclodextrins is too small, and the use of g-cyclodextrins is often limited by their high production cost. Therefore, b-cyclodextrins are used more widely in various industries compared to other natural cycle about dextrins: they have a satisfactory cavity size, and their production and purification are more economical [Lovatti Alves Q. et al. Drugs-b- Cyclodextrin inclusion complex: would be a new strategy to improve Antihypertensive Therapy //Clin. Res. trials. - 2019. - T. 5. - C. 1-3].
  • b-Cycle o dextrins have various effects, in particular, they regulate the simultaneous absorption and prolonged release of odor molecules, including unpleasant ones, the kinetics of dyeing and the release of dyes during washing, the stabilization of aromatic compounds (flavors, fragrances, essential oils) in formulas and provide thermal stability of household chemicals and perfumes and cosmetics.
  • a major ventilation problem in domestic or commercial kitchens is the removal and separation of volatile compounds that are perceived as strong odors, especially when frying food with vegetable oils and fats.
  • Cone-shaped molecules of b-cyclodextrins allow you to adsorb strong and pungent odors of caproaldehyde, which is a marker of the oxidation of oils and fats during cooking [Ghorani B. et al. Assembly of odour adsorbent nanofilters by incorporating cyclodextrin molecules into electrospun cellulose acetate webs //Reactive and
  • the combination can be used at a constant dosage of lipase and unchanged reaction time, using elevated pressure to homogenize the solution.
  • the composition described above from patent application W01990010687A1 has a number of disadvantages, in particular, the impossibility of using at high temperatures above 60 ° C due to denaturation to the quaternary structure of the enzyme, which leads to its inactivation and a decrease in the efficiency of the lipid hydrolysis process, as well as the use of high pressure more than 0.101325 MPa, which requires special technological equipment and confirmation of lipase activity at a given pressure.
  • the activity of lipase at low temperatures, a wide temperature range (temperature lability) would expand the area of application of this combination.
  • a higher oil/water/lipase ratio would allow scale-up of the process and increase the yield of hydrolysis products since less fat and lipase would be required for the process.
  • the use of lipase of animal origin is also described, which indicates a lack of awareness of the environment and will not allow obtaining voluntary Vegan / Vegetarian certification for household chemicals with this component.
  • a decrease in the amount of lipase in the technological process can be achieved by adding b-cyclodextrins, which act as colipase and an activator of the kinetics of cleavage of triglycerides of fatty acids.
  • the active form of stable, rapidly soluble, free-flowing cyclodextrin in granular form has a deodorizing effect and is able to smooth laundry after washing, mainly cotton, as well as for washing other surfaces such as upholstery, curtains, carpets, blankets, etc.
  • the component can be used in conjunction with enzymes (protease, cellulase, lipase, amylase, and/or a mixture of these enzymes) and excipients.
  • composition from patent application WO01/18163A1 contains only granular cyclodextrins as odor absorbing components, however, it is not capable of targeting fatty acid triglycerides and increasing the effectiveness of detergents in this direction, since lipase is not the main active component of the composition.
  • Granular cyclodextrin due to the average particle size can only be used in the composition of fillers, powder mixtures or laundry detergents for washing clothes, which limits the scope of the component, since it is difficult to introduce it into liquid detergents to achieve this effect due to low solubility.
  • non-granular cyclodextrins dissolve more easily in water with stirring and are stable in liquid detergents, which expands the scope of the components. Additionally, non-granulated cyclodextrins would absorb the products of the hydrolysis reaction of lipid substrates, would make it possible to regulate the kinetics of fat breakdown, would absorb unpleasant odor molecules due to the hydrophobic cavity, and would reduce the amount of water and electricity consumed per 1 wash of linen.
  • the component can be used in conjunction with a system of surfactants, enzymes (protease, cellulase, lipase, amylase and / or a mixture of these enzymes), bleaching agents, fillers, releasing agents, optical brighteners, fabric softeners, dispersing agents, dye leaching inhibitors from fabrics , abrasives, bactericidal substances, fragrances.
  • the composition based on the modified transferase is aimed at removing body sebum and contaminants based on plant fibers, sugars, amylose, which are difficult to remove during washing, especially at low temperatures.
  • a need has been described for a composition for removing soil, smoothing and softening laundry, controlling static, maintaining color, and improving fabric durability.
  • composition described above from patent application EP1075509A1 (W09957254A1) is designed to specifically cleave polysaccharide substrates, in particular glycosidic dimers, oligomers and/or polymers from starch, xyloglycans, cyclodextrins, sucrose and maltose. Cyclodextrins do not increase the effectiveness of the composition, since they are substrates for the enzyme that breaks them down.
  • the enzyme lipase is described as a possible additional enzyme in this compositions along with protease, cellulase, amylase. In the examples given, the content of lipase varies from 0.002 to 0.01%, which does not completely break down lipid contaminants in short laundry programs (up to 30 minutes).
  • the composition does not have the ability to specifically absorb unpleasant odors that are fixed by sebum and lipid deposits, but only partially removes them during washing and drying clothes.
  • the use of a lipase enzyme with high activity and an increase in its content would increase the efficiency of removing fatty contaminants and eliminating odors due to the presence of specific esters responsible for unpleasant odors, and non-granular cyclodextrins would absorb the products of the hydrolysis reaction of lipid substrates, would allow regulating the kinetics of fat breakdown, would absorb unpleasant odor molecules due to the hydrophobic cavity and would reduce the amount of water and electricity consumed per wash.
  • the combination is safe on the skin of the hands and maintains dermatological comfort of the skin, which allows to reduce the content of anionic surfactants that cause an increase in the sensitivity of the skin of the hands, and does not cause an irritant effect. Due to the synergistic effect of lipase and b-cyclodextrins, it becomes possible to reduce the content of anionic surfactants or combinations with them by 30% or more.
  • the addition of b-cyclodextrins makes it possible to reduce the reaction temperature and reduce the peroxidation of triglycerides of unsaturated fatty acids, which are contained in soybean, corn, linseed, olive and other edible oils.
  • the technical result of the innovative complex is the effective removal of fatty contaminants due to the regulation of the kinetics of lipid cleavage contaminants, eliminate unpleasant odors and maintain the thermal stability of samples.
  • the complex is active in a wide pH range, in particular 5.0-10.0 units, and a wide temperature range, in particular from +5 to +60°C, which expands the scope of application in detergents and cleaners.
  • lipase and b-cyclodextrins have been found to increase the removal efficiency of complex protein-lipid soils on various surfaces, in particular metal, polymer, glass and wood surfaces, as well as increase the cleaning efficiency of products by more than 35% on various surfaces while maintaining the content of surfactants based on components of natural origin.
  • lipase and b-cyclodextrins are understood as raw materials containing the corresponding active components, as well as technical impurities that could be formed during the production of the target raw material.
  • Lipase obtained by modern methods of biotechnology without the use of GMOs, is an active enzyme that breaks down insoluble lipid substrates with triglyceride ester bonds at the water-oil or oil-water interface.
  • b-Cyclodextrins act as coactivators (colipases) by binding to the terminal domain of lipase and forming a clathrate shell from water molecules, contributing to a change in the conformation of the active center of the lipase and reducing the activation energy of the process (AE a ), which facilitates the enzymatic reaction.
  • the lipase enzyme lowers the activation energy by increasing the number of activated fatty acid triglyceride and glycerol molecules, which become reactive at a lower energy level, which lowers the energy barrier to further enzymatic reaction.
  • cyclodextrins due to the hydrophilic surface due to free hydroxyl groups -OH, specifically orient lipase substrates at the water-oil or oil-water interfaces, increasing the availability of the ester bond for enzymatic hydrolysis. Since the enzymatic reaction is an equilibrium one, in order to shift the equilibrium of the reaction towards the formation of hydrolysis products, it is necessary to bind and remove free fatty acids from the system.
  • b-cyclodextrins bind the reaction products due to their emulsifying properties, shifting the reaction equilibrium towards the breakdown of lipid substrates in the system.
  • An additional property is the neutralization of odorous aromatic substances represented by various classes of organic substances: aldehydes and ketones, terpenes, amines, indole, sulfur-containing components (mercaptans), organic acids and their esters, phenols and cresols. Since a person is more sensitive to unpleasant odors, the neutralization of odorous substances becomes the main tool for managing the emotional state. In such cases, cleaning with conventional household chemicals does not provide effective odor removal, so there is a need for a special combination to control and eliminate odors in addition to regulating the kinetics of hydrolysis of lipid contaminants.
  • An innovative complex including lipase and b-cycle o dextrins, is aimed at effective cleansing and removal of fatty contaminants by regulating the kinetics of the cleavage reaction of lipid substrates and increasing the washing efficiency of household chemicals, as well as neutralizing unpleasant odors.
  • the complex based on natural and biodegradable components is effective in the pH range of 5.0-11.0 in the presence of various synthetic and natural components, which allows it to be used in a wide range of household chemicals for cleaning various surfaces, such as laundry detergents, washing dishes, floors, glasses, universal cleaners.
  • the components target lipid and complex contaminants on various surfaces, and also bind the released metabolic products with an unpleasant odor for humans. After applying the composition, the cleanliness of surfaces and a pleasant aroma are preserved longer.
  • the combined use of the components in the claimed composition in one product leads to an increase in the kinetics of the enzymatic reaction of the breakdown of complex household soiling by lipids due to stabilization, orientation of substrates and an increase in lipase activity due to b-cyclodextrins, which allows for rapid cleansing even under cold water conditions.
  • a distinctive feature is that the components in the claimed concentrations act only on complex contaminants and do not violate the consumer appearance of most surfaces, in particular metal, wood, polymer and enamel.
  • the composition does not contain aggressive inorganic substances and organic solvents, occlusive film formers and alcohols, so it is possible to use detergents and cleaners with this composition on a regular basis without harm to the skin of human hands.
  • the combined use of these components exhibits a synergistic effect that provides complete care. behind various surfaces in one household chemical product for daily use.
  • the invention generally relates to a composition and its use, allowing to achieve such technical results as the effective regulation of the kinetics of enzymatic degradation of lipids while effectively neutralizing unpleasant odors on various types of surfaces, including especially metal, polymer, enamel, glass and wooden, with long-term cleanliness and pleasant aroma, which are not achieved or insufficiently achieved by modern commercially available means in this field of technology.
  • the invention relates to a composition for use in a household product, consisting of lipase and b-cyclodextrin, where the weight ratio of lipase and b-cyclodextrin is (0.0025-0.25):(0. 1-1) respectively.
  • the composition may differ in that the amount of lipase in the specified weight ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0, 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25.
  • the composition may differ in that the amount of b-cyclodextrin in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0 .2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8 , 0.85, 0.9, 0.95 or 1.
  • composition may be characterized in that said lipase is in an aqueous glycerol solution.
  • composition may be characterized in that said aqueous glycerol lipase solution is a commercial product Lipex® Evity® 200 L.
  • Lipex® Evity® 200 L is available, in particular from Novozymes and can be identified, for example, by
  • composition may be characterized in that said aqueous glycerol lipase solution is a commercially available Lipex® Evity® 200 L modified with additional glycerol.
  • composition may differ in that the composition contains Lipex® Evity® 200
  • the composition may differ in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerol, the mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is (0.1-1): (0 .5-1.5), respectively.
  • the composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 .3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9 , 0.95 or 1.
  • the composition may differ in that the amount of additional glycerol introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.5, 0.55, 0.6, 0.65, 0, 7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5.
  • the composition may differ in that it additionally contains decylglucoside.
  • the composition may differ in that said decyl glucoside is a commercially available NaturalAPG HG0814CM product. NaturalAPG HG0814CM is available in particular from Hugo and can be identified for example at ⁇ https://www.hugochem.net/alkyl-polyglucoside/apg-0814/decyl-glucoside-apg-2000up.html>.
  • composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14).
  • the composition may differ in that the amount of Lipex® Evity® 200 L in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5, or 0.6.
  • the composition may differ in that the amount of NaturalAPG HG0814CM in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, or fourteen.
  • the composition may differ in that the composition contains commercially available product Lipex® Evity® 200 L modified with additional glycerol, b-cyclodextrin and commercially available product NaturalAPG HG0814CM, where the content of commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. to the mass of the composition.
  • composition may be characterized in that the activity of said lipase is at least 40 LU/r or 40 LU/ml at pH 7.0.
  • the composition may differ in that said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pretreatment stain remover, laundry gel and fabric softener.
  • said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pretreatment stain remover, laundry gel and fabric softener.
  • the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a floor cleaner, and/or a toilet bowl, and/or a sink, and/or a bathtub, and/or glasses, containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a universal cleaner for cleaning surfaces containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention.
  • the tool for cleaning pipes may differ in that the specified tool contains 0, 0525-1, 5% wt. the specified composition, preferably 0,0525-1%wt. specified composition.
  • the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention.
  • the laundry detergent may be characterized in that said detergent is selected from a delicate laundry detergent and a baby laundry detergent.
  • the laundry detergent may be characterized in that said agent is a powdered laundry detergent.
  • the invention in another aspect, relates to the use of a composition of the invention for regulating the kinetics of enzymatic lipid breakdown and neutralizing unpleasant odors on various surfaces, maintaining long-term cleanliness and pleasant aroma.
  • the application may differ in that said surface is selected from metal, polymer, enamel, glass or wood.
  • the invention relates to a method for preparing a composition according to the invention, comprising preliminary adding a solution of lipase to a dispersion of b-cyclodextrin, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes.
  • auxiliary acceptable substances can be selected from the following categories of components.
  • Salts of higher carboxylic acids with the general formula: R1-C02X1, where R1 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI is an alkali and / or alkaline earth metal, ammonium, alkylammonium cation, alkanolammonium, glucoammonium, basic amino acid;
  • R6 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 4 to 20 carbon atoms
  • X6 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation
  • a mono or disubstituted higher carboxylic acid amide salt of glutamic acid with the general formula: R7-C(0)-NH-CH(-CH2-CH2-C02X7)-C02X7, where R7 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X7 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium or hydrogen cation;
  • Alkyl polyglucoside hydroxypropylsulfonate with the general formula: Rll-O- [G]pl-0-CH2-CH(-0H)-CH2-S03Xl 1 where R11 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, pi can take values from 1 to 4, and XI 1 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;
  • Alkyl polyglucoside carboxylate with the general formula: R12-0-[G]p2-0-CH2-C02X12, where R12 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p2 - can take values from 1 to 4, and XI 2 - cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium; [0080] Higher fatty acid amide salt of threonine with the general formula: R13-C(0)-
  • NH-CH (-CH (-0H) -CH3) -C02X13 where R13 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI 3 is an alkali and / or alkaline earth metal, ammonium cation, alkylammonium, alkanolammonium, glucoammonium;
  • An amide salt of a higher fatty acid and an amino acid derived from the hydrolysis of proteins from plant materials with the general formula: R14-C(0)-AAX14, where R14 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , AA is an amino acid or peptide obtained by hydrolysis of vegetable protein (possible protein sources: apple, soybean, wheat, cotton, etc.), and XI 4 is an alkali and / or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation.
  • Disubstituted salt of acylamphodiacetate with the general formula: R15-C(0)-NH-CH2-CH2-N(-CH2-C02X15)-CH2-CH2-0-CH2-C02X15, where R15 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI 5 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;
  • R23 is an alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms;
  • Alkyl betaine with the general formula: R24-N(-CH3)2)-CH2-C02, where R24 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms
  • Alkylhydroxysultaine with the general formula: R25-N(-CH3)2-CH2-CH(-OH)-CH2-SO3, where R25 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;
  • Alkylsultaine with the general formula: R26-N(-CH3)2-CH2-CH2-CH2-SO3, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;
  • Alkylamine oxide with the general formula: R27-N(-CH3)2-0, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms.
  • Alkyl polyethylene/propylene glycol with the general formula: R30-O(-CH2-CH2-O-)n4(-CH(-CH3)-CH2-0-)n5H, where n4 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, n5 can take values from 2 to 20, and denotes the number of polypropylene glycol groups, R30 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms;
  • R35(-OH)sl An organic alcohol with the general formula: R35(-OH)sl, where R35 is an alkyl group with a long hydrocarbon chain from 3 to 12 carbon atoms, S1 - can take values from 1 to 12, and denotes the number of hydroxyl groups arranged in hydrocarbon radical in an arbitrary order relative to each other;
  • Polysaccharide derivatives carboxymethyl polysaccharide sodium salt, hydroxyalkyl polysaccharide, alkyl polysaccharide;
  • Organic alcohols and phenols phenoxyethanol, benzyl alcohol, caprylyl glycol, ethylhexylglycerin, phenethyl alcohol, 3-methyl-4-isopropylphenol, 2,4-dichlorobenzyl alcohol;
  • Enzymes protease, amylase, pectate lyase, mannanase, cellulase, amine oxidase, nuclease, ferruloyl esterase, gluconase, tannase and other commercially available enzymes that are used in laundry, dishwashing, flooring, glass, all-purpose cleaners .
  • Oxygen based bleaches hydrogen peroxide, calcium peroxide, carbamide peroxide, e-phthalimidoperoxycaproic acid, and other commercially available ingredients.
  • the present invention can be characterized by the following features.
  • the invention relates to a composition for use in a household product, consisting of lipase and b-cyclodextrin, where the weight ratio of lipase and b-cyclodextrin is (0.0025-0.25):(0.1 -1) respectively.
  • the composition may differ in that the amount of lipase in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0, 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25.
  • the composition may differ in that the amount of b-cyclodextrin in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0.2, 0 .25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85 , 0.9, 0.95 or 1.
  • composition may be characterized in that said lipase is in an aqueous glycerol solution.
  • the composition may be characterized in that said aqueous glycerin lipase solution is a commercially available product Lipex® Evity® 200 L.
  • the composition may be characterized in that said aqueous glycerin lipase solution is a commercially available product Lipex® Evity® 200 L modified with additional glycerin.
  • the composition may differ in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerol, and b-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. to the mass of the composition.
  • the composition may differ in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerol, the mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is (0.1-1): (0.5- 1.5) respectively. [0129]
  • the composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 .3, 0.35, 0.4, 0.45, 0.5, 0.55,
  • composition may differ in that the amount of additional glycerol introduced for modification in the indicated mass ratio Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0 .95, 1, 1.1,
  • the composition may be characterized in that it additionally contains decyl glucoside.
  • the composition may differ in that said decyl glucoside is a commercially available NaturalAPG HG0814CM product.
  • the composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14).
  • the composition may differ in that the amount of Lipex® Evity® 200 L in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0.6.
  • the composition may differ in that the amount of NaturalAPG HG0814CM in the indicated weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5 or 14.
  • Composition may differ in that the composition contains a commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerol, b-cyclodextrin and a commercially available product NaturalAPG HG0814CM, where the content of a commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. to the mass of the composition.
  • composition may be characterized in that the activity of said lipase is at least 40 LU/r or 40 LU/ml at pH 7.0.
  • the composition may differ in that said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pre-treatment and laundry stain remover, laundry gel and fabric softener.
  • said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pre-treatment and laundry stain remover, laundry gel and fabric softener.
  • the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a floor cleaner, and/or a toilet bowl, and/or a sink, and/or a bathtub, and/or glasses, containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a universal cleaner for cleaning surfaces containing 0.0525-1% wt. compositions according to the invention.
  • the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention.
  • Means for cleaning pipes may differ in that the specified tool contains 0.0525-1.5% wt. the specified composition, preferably 0,0525 - 1% wt. specified composition.
  • the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention.
  • the laundry detergent may differ in that said detergent is selected from a delicate laundry detergent and a baby laundry detergent.
  • the laundry detergent may be characterized in that said agent is a powdered laundry detergent.
  • the invention relates to the use of the composition of the invention for regulating the kinetics of enzymatic lipid breakdown and neutralizing unpleasant odors on various surfaces, maintaining long-term cleanliness and pleasant aroma.
  • the application may differ in that said surface is selected from metal, polymer, enamelled, glass or wood.
  • the invention relates to a method for preparing a composition according to the invention, including the preliminary introduction of a solution of lipase into a dispersion of b-cyclodextrin, in a solvent, for example, water, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes. This allows a homogeneous solution to be obtained.
  • a mixture consisting of purified water, anionic surfactants 5-15%, nonionic surfactants based on glycosides ⁇ 5%, glycerin, cotton extract, tetrasodium diacetate glutamate, monohydrate citric acid, sodium hydroxide, a preservative, and an aqueous solution of citric acid and silver citrate (Table N°1).
  • the dishwashing detergent base was prepared as follows: the solubilizer glycerin and the chelating agent glutamate diacetate tetrasodium salt were mixed with purified water while stirring in an ordinary mixer until the mixture was homogeneous. Pre-homogenized anionic surfactants with a sufficient amount of water purified by heating 40-60°C. Next, non-ionic surfactants based on glycosides and an anionic surfactant solution were added. Cotton extract and an aqueous solution of citric acid and silver citrate were also added. At the very end, an organic acid was added to adjust the pH, a preservative to maintain microbiological stability throughout the shelf life. The ingredients were mixed until a homogeneous clear solution was obtained. After mixing, the pH of the product was measured and adjusted by adding sodium hydroxide to the desired value.
  • the test procedure is based on OST 6-15-1662-90 "Household cleaning products".
  • the method for determining the washing ability for various surfaces is that the gravimetric method determined the mass of removed artificial oil-fat contamination for a certain time with the test agent in relation to the comparison agent. Glass plates were chosen as surfaces. The glass plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and rubbed with ethyl alcohol. After preparation, the plates were weighed to determine the baseline.
  • a complex oil-fat pollutant consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1:4.9:1.0 :5,1:7,3:2,1:4,5:20 respectively.
  • Weighed lubricants and lanolin were mixed at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled, and egg yolk and other components were added.
  • the contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to an oven and calcined at 220 ⁇ 5°C for 8 minutes. Then cooled and weighed. The samples were used in pure form. As a means of comparison, a standard household chemical product with a similar quantitative composition was used.
  • each plate was placed in a jar with a lid, a nylon fabric was placed on top and 1 jar with a plate was filled with a clean sample of the test agent, another 1 jar with plates was filled with 40 ml of a reference agent to maintain the ratio of water: lipophilic contaminant (fat , oils, glycerides) such as 9:1.
  • the jars were placed in a liquid shaking machine and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.
  • A is the mass of a piece of a plate with a pollutant before washing with a means, g;
  • B is the mass of a piece of a plate with a contaminant on the plates after washing with the agent, g.
  • B is the mass of a clean plate, g.
  • the parameter for the overall assessment of the washing efficiency and the kinetics of the breakdown of lipid contaminants in the composition of the product was the calculation of % washing efficiency, determined by the laboratory assistant based on the results of the study.
  • the weight fraction of contaminant removed measures the cleaning performance against difficult lipid-based soils when the composition is used daily in a dishwashing detergent.
  • the general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the dishwashing detergent and various surfaces.
  • test composition in the composition of the dishwashing detergent has a pronounced washing effect on complex lipid-protein contaminants when compared with a control agent that does not contain components of the claimed composition.
  • the base of the dishwashing detergent composition will gently clean dirt from the surfaces of dishes (glass, metal, polymer, ceramic) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in relation to complex lipid-protein contaminants, including obsolete.
  • the study of a dishwashing detergent with the claimed composition has led to a pronounced synergistic effect in relation to soils that are difficult to remove using conventional surfactant-based dishwashing detergents.
  • the composition makes it possible to replace synthetic surfactants with surfactants of natural origin without losing the effectiveness of the product, which reduces the burden on the environment, maintains an approach to human health and provides a high percentage of natural ingredients in the composition of household chemicals for consumers.
  • composition contains natural biotechnology-derived lipase and natural b-cyclodextrins, a high % removal of complex contaminants with low labor and time savings will be achieved with a single application.
  • Combining the components in the composition makes it possible to regulate the kinetics of the enzymatic cleavage of lipid substrates that fix complex contaminants.
  • Lipase provides targeted cleavage of lipid contaminants in various phase systems
  • cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, providing changes in the conformation of the protein domain with the active center and the correct orientation of substrates.
  • cyclodextrins bind released cleavage products that have an unpleasant smell, which allows you to neutralize odorous aromatic molecules and control the atmosphere in the room for the psychological comfort of a person.
  • the combination of components makes it possible to achieve a synergistic effect in relation to complex lipid-protein contaminants due to the targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows regular use as part of household chemicals for various surfaces.
  • the test method is based on OST 6-15-1662-90 "Household cleaning products".
  • the method for determining the washing ability for various surfaces is that the gravimetric method was used to determine the mass of removed artificial oil-fat contamination for a certain time by the test sample in relation to the reference sample.
  • Metal and plastic plates were chosen as surfaces. 8 metal and 8 plastic plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and rubbed with ethyl alcohol. After preparation, the plates were weighed to determine the baseline.
  • a complex oil-fat pollutant consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1:4.9:1.0 :5,1:7,3:2,1:4,5:20 respectively.
  • Weighed lubricants and lanolin were mixed at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled, and egg yolk and other components were added.
  • the contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to an oven and calcined at 220 ⁇ 5°C for 8 minutes. Then cooled and weighed. The samples were used in pure form. As a means of comparison, distilled water was used, not containing the claimed composition.
  • each plate was placed in a jar with a lid, a nylon fabric was placed on top and 1 jar with a plate was filled with a test sample, another 1 jar with plates was filled with 40 ml of a reference sample to the ratio of water:lipophilic contaminant (fat, oils, glycerides) was 9:1.
  • the jars were placed in a liquid shaking machine and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.
  • A is the mass of a piece of plate with a contaminant before washing with a sample, g;
  • B is the mass of a piece of a plate with a contaminant on the plates after washing with a sample, g.
  • B is the mass of a clean plate, g.
  • the parameter for the overall assessment of the washing efficiency and the kinetics of the breakdown of lipid contaminants in the composition of the product was the calculation of % washing efficiency, determined by the laboratory assistant based on the results of the study.
  • the indicator of the mass fraction of the removed pollutant allows you to evaluate the washing effectiveness in relation to complex lipid-based contaminants (oils and fats with fixing additives) during daily use of the composition in the composition of a dishwashing detergent.
  • the general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the dishwashing detergent and various surfaces.
  • test composition has a pronounced washing effect on complex lipid-protein contaminants on various surfaces when compared with a control sample that does not contain components of the claimed composition.
  • the base of the dishwashing detergent composition will gently clean dirt from the surfaces of dishes (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in relation to complex lipid-protein contaminants , incl. obsolete.
  • the study of the washing effectiveness of the composition showed a pronounced synergistic effect with respect to dirt that is difficult to remove using conventional dishwashing detergents based on natural surfactants.
  • the composition makes it possible to replace synthetic surfactants with surfactants of natural origin or to reduce their content equivalently without losing the effectiveness of the product, which allows reducing the burden on the environment, maintaining an approach to human health and providing a high percentage of natural ingredients in the composition of household chemicals for consumers.
  • composition contains biotechnology-derived lipase and natural b-cyclodextrins, a high % removal of difficult contaminants with low labor and time savings will be achieved with a single application.
  • the combination of components in the composition allows you to control the kinetics of the enzymatic cleavage of lipid substrates that fix complex pollution.
  • Lipase provides targeted cleavage of lipid contaminants in various phase systems
  • cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, providing changes in the conformation of the protein domain with the active center and the correct orientation of substrates.
  • cyclodextrins bind the released breakdown products with an unpleasant odor, which makes it possible to neutralize odorous aromatic molecules and control the atmosphere in the room for the psychological comfort of a person.
  • the b-cyclodextrins act as activity stabilizers and bind the reaction products from the system.
  • the joint use of components retains high activity at different % input of the composition, depending on the type of household chemicals and its composition.
  • the combination of components makes it possible to achieve a synergistic effect on complex lipid-protein contaminants due to the targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows regular use as part of household chemicals for various surfaces.
  • test methodology is based on Deodorizing Ability of Houttuynia cordata Thunb. (Dokudami) for Masking Garlic Odar - Hiromi Ikeura and allows you to evaluate the ability of the components to neutralize unpleasant odors by organoleptic method.
  • test composition in the composition of household chemicals has a pronounced deodorizing effect compared to a reference sample that does not contain components of the claimed composition.
  • Each of the components contributes to the overall effect, and their combination allows to achieve the neutralization of unpleasant odors up to 100%, depending on the concentration of substances according to the invention (Table JV ° 5).
  • the synergistic effect of lipase and b-cyclodextrins is manifested in an increase in the effectiveness of eliminating unpleasant odor by +0.5 points, which is a good change when combining the components in addition to the effects described in examples 1 and 2.
  • the base of the composition of the universal surface cleaner will gently clean dirt from surfaces (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in neutralizing persistent unpleasant odors.
  • the study of the deodorizing effect of the composition showed a pronounced synergistic effect in relation to unpleasant odors that are difficult to mask or wash off with conventional surface cleaners based on natural surfactants.
  • the composition makes it possible to replace synthetic unpleasant odor absorbers, synthetic fragrances or aromatic compositions in the composition of the product without loss of effectiveness, which makes it possible to maintain a safe effect on human health, incl. people with allergic respiratory diseases, and provide a high percentage of natural ingredients in the composition of household chemicals for consumers.
  • composition contains biotechnology-derived lipase and natural b-cyclodextrins, a high % neutralization of unpleasant odors due to sulfur-containing compounds will be provided with a single application.
  • components mercaptans
  • organic acids their esters and other compounds.
  • the combination of components in the composition allows you to specifically eliminate the cause of unpleasant odors, and not temporarily mask with the help of aromatic substances with a more pronounced intensity of perception.
  • the combination of components allows to achieve a synergistic effect in relation to persistent unpleasant odors due to targeted action and high affinity for organic odor molecules, which allows you to regularly use it as part of household chemicals for various surfaces and control the atmosphere in the room for the psychological comfort of a person .
  • a water-glycerin solution of the components is introduced with active stirring into the medium with the substrate and incubated at 30°C for 4 hours.
  • the initial pH of the solution was 8.20 ⁇ 0.10.
  • b-cyclodextrins interact with amino acid residues of lipase subunits, forming clathrate complexes on the surface of the enzyme, which changes the microenvironment chromophore groups and thereby changes the ability to absorb and emit light quanta in different regions of the spectrum.
  • optical density of the peak at 270–290 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returns to its original value, which indicates the formation of a stable clathrate complex between lipase and b-cycle o-dextrins. It should be noted that the optimal ratio of lipase-aciclodextrin is from 1:15 to 1:40 without changing the effectiveness of the composition.
  • the emission spectra of fluorescence quanta were determined at a wavelength of 280-450 nm and the relative fluorescence intensity F was calculated, indicating an energetically favorable conformation of the enzyme and the ability of amino acid residues to absorb and emit light quanta. According to the results of the study, it was found that at an absorption maximum of 350-360 nm, the emission spectrum does not change when a dispersion of b-cyclodextrins is added.
  • the fluorescence intensity of the peak at 340–350 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returns to its original value, which indicates the formation of a stable clathrate complex between lipase and b-cyclodextrins. It should be noted that the optimal ratio of lipase-aciclodextrin is from 1:15 to 1:40 without changing the effectiveness of the composition.
  • the method of obtaining funds according to the invention includes the following steps.
  • the main process of preparing household chemicals is preceded by the stage of preliminary application of a lipase solution to a dispersion of b-cyclodextrins.
  • the powder of b-cyclodextrins is added to the dispersion medium, the ratio of cyclodextrins to the dispersion medium is 1:1.20-2.50.
  • the process is carried out at room temperature and atmospheric pressure. Cycle o dextrins are added in small portions, with continuous stirring. After adding the last portion of cyclodextrins, the dispersion is stirred for 10-15 minutes, the signal for the transition to the next stage of the process is the absence of large inclusions of cyclodextrins in the dispersion. Then, a lipase solution is added to the resulting dispersion in small portions, the ratio of cyclodextrins (by dry matter) to the lipase solution is 1: 0.5-
  • the resulting dispersion of lipase and b-cyclodextrins is dissolved in the main reactor to prepare the finished household chemicals, for example, dishwashing detergent.
  • the dispersion is pumped into preheated water, the resulting solution is mixed until completely dissolved.
  • a high-speed disperser is used to intensify dissolution.

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Abstract

L'invention concerne une composition que l'on utilise dans la chimie domestique, et qui se compose de lipase et de bêta-cyclodextrine, dans lequel le rapport en poids de la lipase et de la bêta-cyclodextrine est de (0,0025-0,25):(0,1-1), respectivement.
PCT/RU2021/000271 2021-05-18 2021-06-28 Composition détergente à base de lipase et de bêta-cyclodextrine WO2022245243A1 (fr)

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WO1990010687A1 (fr) 1989-03-08 1990-09-20 Novo Nordisk A/S Procede d'hydrolyse de matiere grasse
WO1999057254A1 (fr) 1998-05-01 1999-11-11 The Procter & Gamble Company Compositions de detergent a lessive et/ou d'entretien de tissus contenant une transferase modifiee
WO2001018163A1 (fr) 1999-09-03 2001-03-15 The Procter & Gamble Company Composition detergente comprenant de la cyclodextrine granulaire
WO2017091674A1 (fr) * 2015-11-26 2017-06-01 The Procter & Gamble Company Compositions de détergent liquide comprenant une protéase et une lipase encapsulée
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WO2020074302A1 (fr) * 2018-10-12 2020-04-16 Unilever N.V. Composition de nettoyage comprenant une silicone augmentant le pouvoir moussant

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Publication number Priority date Publication date Assignee Title
JPH01256596A (ja) * 1988-04-06 1989-10-13 Kao Corp 粉末洗浄剤組成物
WO1990010687A1 (fr) 1989-03-08 1990-09-20 Novo Nordisk A/S Procede d'hydrolyse de matiere grasse
EP0427806B1 (fr) 1989-03-08 1994-06-29 Novo Nordisk A/S Procede d'hydrolyse de matiere grasse
WO1999057254A1 (fr) 1998-05-01 1999-11-11 The Procter & Gamble Company Compositions de detergent a lessive et/ou d'entretien de tissus contenant une transferase modifiee
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