WO2022245243A1

WO2022245243A1 - Detergent composition based on lipase and beta-cyclodextrin

Info

Publication number: WO2022245243A1
Application number: PCT/RU2021/000271
Authority: WO
Inventors: Елена Юрьевна БЕЛОУС; Виктор Андреевич ФИЛАТОВ
Original assignee: "Скайлаб At"
Priority date: 2021-05-18
Filing date: 2021-06-28
Publication date: 2022-11-24
Also published as: EP4342968A1

Abstract

The invention relates to a composition for use in a household cleaning agent, consisting of lipase and ꞵ-cyclodextrin, where the mass ratio of lipase to ꞵ-cyclodextrin is (0.0025-0.25):(0.1-1).

Description

DETERGENT COMPOSITION BASED ON LIPASE AND BETA-

CYCLO DEXTRINA

FIELD OF TECHNOLOGY

[0001] The invention relates to a composition and its use in a household chemical product, where the composition consists of lipase and b-cyclodextrin, and the mass ratio of lipase and b-cyclodextrin is (0.0025-0.25): (0.1-1) respectively. EFFECT: invention provides effective regulation of lipid enzymatic cleavage kinetics with simultaneous effective neutralization of unpleasant odors on various types of surfaces, including especially metal, polymer, enameled, glass and wood, while maintaining long-term cleanliness and pleasant aroma.

BACKGROUND OF THE INVENTION [0002] The development of household chemicals with improved properties for the consumer remains one of the promising areas in the category of home care products. Experts estimate that in the UK alone, by the end of 2020, the category of cleansers will grow by 9% CAGR and amount to 485 million pounds compared to 2019 [Dishwashing products: Impact of COVID-19, UK, July 2020, Mintel], and in China, the market volume is growing at a 4% CAGR annually and will reach RMB17,195 million by 2024 [Dishwashing Products - China - February 2020, Mintel]. [0003] Every day, a person uses a sufficient amount (> 3) of household chemicals to clean various surfaces of the house: wood, ceramic, enamel, metal, polymer, porcelain, etc. Consumers more often pay attention to the functional characteristics of the product of choice, in particular to the removal efficiency household pollution (types of pollution, difficulty of their removal, speed of cleaning), additional effects, for example, elimination of unpleasant odors in the house, a safe effect on the surfaces being cleaned and the skin of hands, as well as environmental friendliness and the possibility of using products in homes with independent sewerage and septic tanks. Based on a March 2019 Lightspeed/Mintel survey, over 38% of UK consumers prefer to choose multifunctional household chemicals that reduce the amount of time required for a full cleaning. By 2020-2025 Mintel, Dishwashing products: Impact of COVID-19, UK, July 2020 there will be a growing trend for sustainable products with a high content of natural ingredients, a long list of ingredients that are not contained in products (free from list). It should be noted that the consumption of household chemicals in the EMEA region does not depend on the income level of the population, since such products are essential goods. However, according to a Lightspeed/Mintel survey in March 2020, 10-16% of consumers over the age of 18 have become less likely to use household chemicals due to low efficiency (10% of respondents), high content of synthetic chemicals (11% of respondents), high water consumption to remove contaminants (36% of respondents). Additionally, an assessment was made of the attractiveness and possibility of buying household chemicals in the EMEA region. Thus, the main stimulating factors are a decrease in physical effort to remove contamination by hand (more than 33% of responses), a decrease in water consumption for washing off contamination (more than 28% of responses) and a high confirmed effectiveness of the product (more than 27% of responses). Thus, the need has been identified for the development of innovative household chemicals that can effectively remove household pollution, reduce the effort spent on cleaning surfaces and are safe for nature and humans.

[0004] However, despite the demand for effective products, consumer attention remains to the dermatological comfort of the skin of the hands after using cleaning and household detergents. Every day, a person comes into contact with a large number of surfaces in public places and at home, and also uses household chemicals. In order to protect yourself from the development of dermatological diseases, it is necessary to take care of the choice of household chemicals, since it is the dermatological comfort of the hands that is the key to health and, accordingly, a high quality of life for people. Due to the frequent contact of hands with synthetic ingredients in skin care products, formulating products with a high content of natural ingredients remains the most reliable way to support healthy skin and, consequently, a healthy appearance. According to experts, skin health is one of the fundamental foundations of overall human health. So, 2% sodium lauryl sulfate, known as SLS, in the composition of household chemicals can cause a loss of transepidermal moisture by 68.9 g/m2/h relative to normal level 12 hours after contact with the agent [Loffler, H., & Happle, R. (2003). Profile of irritant patch testing with detergents: sodium lauryl sulfate, sodium laureth sulfate and alkyl polyglucoside. Contact Dermatitis, 48(1), 26-32]. Since the content of sodium lauryl sulfate in household chemicals can reach up to 29% due to the high washing power, this can adversely affect the condition of the skin of the hands. C 10-16 alkyl sulfates cause denaturation of keratin by destroying sulfide bonds and forming -SH sulfhydryl groups on human cells and washing out the epidermal barrier [Prottey C, Ferguson T. Factors which determine the skin irritation potential of soaps and detergents. J Soc Cosmetic Chem. 1975;26: 29-46.], while alkyl sulfates with a chain length of C 12-C 14, which include SLS, have the maximum activity. It was found that aggressive anionic surfactants sodium dodecyl sulfate (SDS), sodium myristyl sulfate (SMS), sodium tridecyl sulfate (STS) cause leaching of water-soluble proteins of the epidermis by 166.1%, 163.9% and 198.5%, respectively [Loffler, N., & Happle, R. (2003). Profile of irritant patch testing with detergents: sodium lauryl sulfate, sodium laureth sulfate and alkyl polyglucoside. Contact Dermatitis, 48(1), 26-32]. Thus, the development of formulas for detergents and cleaners with household chemicals with a reduced content of synthetic and semi-synthetic anionic surfactants is a priority for caring for the epidermis of the skin of the hands.

[0005] The search for effective components and their combinations as agents for removing household contaminants of various nature, in particular lipid contaminants, is one of the priorities of companies involved in the production of detergents and cleaning products. The main components of household chemicals are surfactants, fillers, components that ensure the stability of formulations, and functional additives that perform specific functions. In particular, special functional additives include lipase and beta-cyclodextrins.

[0006] Lipase or triacylglycerol-acyl-gyrolase (enzyme classification code E.C.3.1.1.3, CAS 9001-62-1, EINECS 232-619-9) is a water-soluble enzyme from the class of hydrolases that catalyzes the hydrolysis of ester bonds into triglycerides of fatty acids, which are water-insoluble esters of glycerol and higher carboxylic acids of various structures [European Commission Cosmetic Ingredients & Substances Database: http://ec.europa.eu/growth/tools-databases/cosing/]. Lipase is registered as a dietary supplement E1104 and can be used to digest, dissolve and fractionate fats. [0007] Lipase is a catalyst for the fat splitting reaction and is not a product/starting material in the hydrolysis reaction, which improves the kinetics of the enzymatic reaction. It is known that one enzyme molecule can catalyze up to 10,000 reactions per second, depending on domain organization, functional activity, and raw materials. A small amount of enzyme is sufficient for effective action, since the activity of commercially available lipases is above 40 U/g or U/ml. Thus, the effective concentration of lipase is 0.4-0.8% (w/w) as part of complex additives for implementation in household chemicals [https://www.enzymeinnovation.com/lipase-detergent-everything-you-need- know/].

[0008] It is believed that lipases are one of the most effective enzymes for removing greasy stains and lipid contaminants based on vegetable oils (sunflower, olive, rapeseed, corn, linseed, etc.) with different fatty acid composition, animal fats (butter, lard, beef fat, mutton fat, etc.), having a solid state of aggregation under normal conditions, lubricants based on ester components and fatty phases of perfumery and cosmetic products containing oils, waxes, emulsifiers with ester bonds, such as lecithin, perfume components and essential oils. Since these stains are insoluble in the aqueous phase and are removed only with the help of micellar solutions of surfactants with limited solubility, lipase promotes the hydrolysis of ester bonds and the destruction of substrates, due to which surfactants of any nature better remove contaminants from the surface, penetrate deep into the tissue and reach saturation of micelles more slowly while maintaining washing efficiency.

[0009] Lipases are highly active against lipid contaminants on various surfaces, including metal, polymer, wood, ceramic, and do not disrupt the structure of these surfaces, which indicates a gentle effect in the composition of detergents. The most desirable pH optimum is between 5.0 and 11.0, and the activity can be maintained at various temperatures from 0°C to 60°C, in particular the temperature optimum is from 20°C to 40°C. Lipases can be stable in the presence of proteases, chelating agents, peroxide compounds (hydrogen peroxide, sodium percarbonate, etc.) and surfactants, in particular anionic ones. Lipase stabilizers, in particular glycerin, propylene glycols, sorbitol, sugars, carboxylic acids, alkylamines, inorganic salts, nonionic and ionic surfactants, as well as a reduced water content in the formulation, can be introduced into the systems, which makes it possible to make the detergent concentrated and with low water consumption in the production of detergents. The use of lipase allows the production of energy-efficient products that save electricity and water, thereby responsibly using resources.

[0010] The concentration of lipase in household chemicals depends on the activity and can range from 0.0025 to 1% (by weight, in pure form). It was found that the addition of a lipase with an activity of 100 KLU3/g to surfactants in the composition of the laundry detergent showed high efficiency in removing grease stains with a standard wash of 40*C. The effective concentration of lipase (as part of complex additives) with this activity is 0.2-0.6% [N. Uhlig, E. M. Linsmaier-Bednar.

industrial enzymes and their applications. Engineering, April 1998, 472 pages. ISBN: 978-0-

471-19660-0. D01.10.5860/choice.36-0333]. In detergent formulations, lipase with an activity of 50 U/mL in an amount of 10 mL, together with a 0.5% anionic and nonionic surfactant system, was stable in the formulation and did not affect the thermal stability of the detergent. Also, lipase was not destroyed in the presence of 2% hydrogen peroxide solution, i.e. retained its activity by 92% after 2 hours of adding bleach to the solution, however, the activity dropped significantly in the presence of sodium hypochlorite and sodium perborate when the concentration was increased from 1 to 2%.

[OOP] Lipase is noted to be a necessary component in detergents for the effective removal of lipid stains and grease. It is the enzymatic activity per unit volume that determines the working concentration of the component in the formulations of detergents for various purposes. With a temporary activity of 50 U/mL and in an amount of 10 mL, the total enzyme activity will be 500 U. With an average enzyme activity of 100-1000 U/g, the effective concentration of lipase (as part of a complex supplement) will be 0.05-5.00 % in detergent formulation [Mamta Chauhan, Rajinder Singh Chauhan, and Vijay Kumar Garlapati. Evaluation of a New Lipase from Staphylococcus sp. for Detergent Additive Capability. BioMed Research International Volume 2013 |Article ID 374967 | 6 pages | https://doi.org/10.1155/2013/374967. https://www.hindawi.com/joumals/bmri/2013/374967/]. [0012] Thus, lipase is a necessary component in detergents to effectively remove lipid stains and grease by targeting fatty acid triglyceride molecules and improving the effectiveness of detergent systems based on surfactants, in particular nonionic surfactants. With an average component activity >100 U/g, the effective concentration of lipase in its pure form is 0.0025-0.1% in cleaners and detergents for cleaning various surfaces.

[0013] However, lipase has a specific odor. Lipase breaks down fats into glycerol and fatty acids. If, for example, these fatty acids are not completely removed from textiles during the washing process, consumers notice a rancid odor that becomes more intense depending on the amount of lipase. (Averyanova V. A. Evolution of washing properties: striving for a sustainable ideal / Raw materials and packaging. 02 (151), 2014). Thus, lipase can lead to irritation of the mucous membranes of the upper respiratory tract and lungs and lead to an allergic reaction. The inventors have successfully overcome this limitation by creating an effective combination.

[0014] Another functional biodegradable component are cyclodextrins. The direction of controlling aromas and atmosphere in the house remains relevant and is actively developing. Smell plays an important role in human life, as it allows you to recognize various compounds and navigate in space, as well as protect yourself from toxic and allergenic substances. It is not always possible to neutralize the unpleasant smell in the room, which causes a feeling of discomfort and does not create a sense of security for a person. Not all household cleaning products have the ability to neutralize unpleasant odors after a thorough cleaning of the home, so there is a need to manage odors to create a comfortable environment.

[0015] Cyclodextrins are substances of natural origin, cyclic glucose oligomers obtained enzymatically from starch [Crini G. A history of cyclodextrins //Chemical reviews. -2014. - T. 114. - 21. - S. 10940-10975]. The o-dextrin cycle contains at least six D-(+) glucopyranose residues linked by a-(1,4)-glucosidic bonds. Glucopyranose residues form an energetically favorable "chair" conformation, which is why cyclodextrin molecules have the shape of cones. The outer surface of the latter is hydrophilic and allows them to be water-soluble, while the central cavity has hydrophobic properties due to the specific arrangement of hydroxyl groups and allows the incorporation of hydrophobic or amphiphilic substances. Natural cyclodextrins are of three types: a, b and g, corresponding to 6, 7 and 8 glucopyranose residues [B. Bonthagarala, CH BABU RAO, N. Sreekanth. The cyclodextrins: A review// IJPRBS - 2013. - T. 2. - S. 291 - 304]. Each of the three types of cycle o dextrins has its own physical and chemical properties, in particular, molecular weight, central cavity diameter, approximate volume cavities and solubility in water, the number of water molecules contained in the cavity of cyclodextrins and pKa of the substance in the aquatic environment. Thus, b-cyclodextrin molecules have the largest internal diameter of the cavity, which allows them to form inclusion complexes (“host-guest” complexes) with a wide range of solid, liquid and gaseous compounds through molecular complexation. In these complexes, the guest molecule is retained in the cavity of the cyclodextrin host molecule due to hydrophobic and van der Waals interactions. The hydrophobic cavity of cyclodextrin molecules provides a microenvironment in which non-polar fragments of suitable size can be introduced to form an inclusion complex [Loftsson T., Brewster ME Pharmaceutical applications of cyclodextrins. 1. Drug solubilization and stabilization // Journal of pharmaceutical sciences. - 1996. - T. 85. - N°. 10. - C. 1017-1025]. In this case, the formation or destruction of covalent bonds that lead to the destruction of molecules does not occur. The main driving force of complex formation is the release of water molecules from the cavity of cyclodextrins rich in DH enthalpy. Water molecules are displaced by a more hydrophobic "guest" under the influence of non-covalent forces of intermolecular interaction, due to which the AG free energy of the surface of the cyclodextrin ring decreases, and the cyclodextrin molecule is stabilized in a low-energy state [Szejtli J. Introduction and general overview of cyclodextrin chemistry //Chemical reviews. - 1998. - T. 98. - 5. - C. 1743-1754]. The binding of "guest" molecules is characterized by dynamic equilibrium. The strength of the binding depends on how strong the non-covalent host-guest interactions are. The complexes can form either in solution or in the crystalline state, with water usually being the preferred solvent [Hadaruga NG et al. A review on thermal analyzes of cyclodextrins and cyclodextrin complexes //Environmental Chemistry Letters. - 2019. -T. 17.-l. - C. 349-373].

[0016] Complexation allows you to change some properties of the "guest" molecules: increase the solubility of hydrophobic molecules, increase the stability of labile molecules, reduce the volatility of aromatic compounds [B. Bonthagarala, CH BABU RAO, N. Sreekanth. The cyclodextrins: A review// IJPRBS - 2013. - T. 2. - S. 291-304]. The list of potential guests for molecular encapsulation in the o ring of dextrins is quite diverse and includes compounds such as straight or branched chain aliphatic compounds, aldehydes, ketones, alcohols, organic acids, fatty acids, aromatics, gases, and polar compounds such as halogens, hydroxy acids and amines [Del Valle E. M. M. Cyclodextrins and their uses: a review

//Process biochemistry. - 2004. - T. 39. - 9. - C. 1033-1046].

[0017] For many encapsulated substances, the cavity size of a-cyclodextrins is too small, and the use of g-cyclodextrins is often limited by their high production cost. Therefore, b-cyclodextrins are used more widely in various industries compared to other natural cycle about dextrins: they have a satisfactory cavity size, and their production and purification are more economical [Lovatti Alves Q. et al. Drugs-b- Cyclodextrin inclusion complex: Would be a new strategy to improve Antihypertensive Therapy //Clin. Res. trials. - 2019. - T. 5. - C. 1-3].

[0018] b-Cycle o dextrins have various effects, in particular, they regulate the simultaneous absorption and prolonged release of odor molecules, including unpleasant ones, the kinetics of dyeing and the release of dyes during washing, the stabilization of aromatic compounds (flavors, fragrances, essential oils) in formulas and provide thermal stability of household chemicals and perfumes and cosmetics.

[0019] Residual surfactants on the surface of the fibers affect the absorbency of the textile surface and the coloring power of textiles, and also reduce the quality of the hydrophobic finish. Cyclodextrins are able to form complexes with surfactants in aqueous solution, which allows you to remove most of the absorbed surfactants. The addition of cycle o dextrin (3 g/l) to rinsing water eliminates residual traces of detergent in solution and thus reduces residual detergent in the fabric (from 209 to 134 ppm) and water consumption [Ammayappan L. , Moses J. J. An overview on application of cyclodextrins in textile product enhancement // J Text Assoc. - 2009. - T. 70. - l. - C. 9-18].

[0020] Promising uses of cyclodextrins in tissue staining have been described in detail [Bezerra F. M. et al. The Role of b-Cyclodextrin in the Textile Industry // Molecules. - 2020. - T. 25. - Zh 16. - C. 3624]. Cyclodextrins, when used as coloring aids, affect both the properties of the dyes and the kinetics of dyeing, allowing an increase in the degree of extraction of excess dye and, thereby, improving the quality of wastewater and the environmental burden. [0021] Molecules or functional groups that cause an unpleasant taste or smell can be hidden from sensory receptors by enclosing them in a cavity of cyclodextrins.

Such complexes significantly reduce the concentration of free molecules that cause an unpleasant taste or smell [Tiwari G., Tiwari R., Rai A. K. Cyclodextrins in delivery systems: Applications //Journal of Pharmacy and Bioallied Sciences. - 2010. - T. 2. -

no. 2. - C. 72], in particular dihydroxyacetone, free fatty acids and esters, amine derivatives, glutathione, mercapto compounds, caproic aldehyde as a marker of the oxidation of vegetable oils and fats. Cyclodextrins, through complexation with aromatic compounds, can be used to mask strong odors or reduce the intensity of odors.

[0022] A major ventilation problem in domestic or commercial kitchens is the removal and separation of volatile compounds that are perceived as strong odors, especially when frying food with vegetable oils and fats.

Cone-shaped molecules of b-cyclodextrins allow you to adsorb strong and pungent odors of caproaldehyde, which is a marker of the oxidation of oils and fats during cooking [Ghorani B. et al. Assembly of odour adsorbent nanofilters by incorporating cyclodextrin molecules into electrospun cellulose acetate webs //Reactive and

functional polymers. - 2019. - T. 134. - C. 121-132], in the process of washing various kitchen surfaces and utensils after frying food. Since fats and oils contain esters of fatty acids and glycerol, a complex based on a specific lipase enzyme and b-cyclodextrins improves the washing process due to the targeted breakdown of fat molecules and the absorption of released fatty acids. This synergistic action improves the cleaning power of household chemicals for various surfaces by regulating the kinetics of fat breakdown and absorbing unpleasant odors caused by fatty acids and their oxidation products, unlike other detergents.

[0023] Documents EP0427806B1 [NOVO NORDISK A/S] publ. 06/20/1994 and W01990010687A1 publ. 09/20/1990 describes the process of hydrolysis of fats in the presence of lipase in water in a ratio of 1:3 to 3:1 (by weight), characterized by the presence of a-, b- or gamma-cyclodextrins, which can be optionally replaced. Cyclodextrins improve the degree of hydrolysis of fats or oils, i.e. any material with a high content of triglycerides, using lipase in an aquatic environment. The combination can be used at a constant dosage of lipase and unchanged reaction time, using elevated pressure to homogenize the solution. [0024] The composition described above from patent application W01990010687A1 has a number of disadvantages, in particular, the impossibility of using at high temperatures above 60 ° C due to denaturation to the quaternary structure of the enzyme, which leads to its inactivation and a decrease in the efficiency of the lipid hydrolysis process, as well as the use of high pressure more than 0.101325 MPa, which requires special technological equipment and confirmation of lipase activity at a given pressure. The activity of lipase at low temperatures, a wide temperature range (temperature lability) would expand the area of application of this combination. A higher oil/water/lipase ratio would allow scale-up of the process and increase the yield of hydrolysis products since less fat and lipase would be required for the process. The use of lipase of animal origin is also described, which indicates a lack of awareness of the environment and will not allow obtaining voluntary Vegan / Vegetarian certification for household chemicals with this component. A decrease in the amount of lipase in the technological process can be achieved by adding b-cyclodextrins, which act as colipase and an activator of the kinetics of cleavage of triglycerides of fatty acids.

[0025] In patent application WO01/18163A1 [THE PROCTER & GAMBLE COMPANY] publ. On August 21, 2000, the use of a composition for the preparation of a laundry detergent containing a granular o-dextrin cycle was disclosed. The composition ensures the elimination of unpleasant odors from things associated with the presence of sweat particles, sebum and sebum, dead skin cells, in the process of automatic washing in the presence of cyclodextrin in the detergent composition. The active form of stable, rapidly soluble, free-flowing cyclodextrin in granular form has a deodorizing effect and is able to smooth laundry after washing, mainly cotton, as well as for washing other surfaces such as upholstery, curtains, carpets, blankets, etc. The component can be used in conjunction with enzymes (protease, cellulase, lipase, amylase, and/or a mixture of these enzymes) and excipients.

[0026] The above-described composition from patent application WO01/18163A1 contains only granular cyclodextrins as odor absorbing components, however, it is not capable of targeting fatty acid triglycerides and increasing the effectiveness of detergents in this direction, since lipase is not the main active component of the composition. Granular cyclodextrin due to the average particle size can only be used in the composition of fillers, powder mixtures or laundry detergents for washing clothes, which limits the scope of the component, since it is difficult to introduce it into liquid detergents to achieve this effect due to low solubility.

The introduction of a specific lipase enzyme would make it possible to increase the efficiency of removing fatty contaminants and eliminating odors due to the presence of specific esters responsible for unpleasant odors, and non-granular cyclodextrins dissolve more easily in water with stirring and are stable in liquid detergents, which expands the scope of the components. Additionally, non-granulated cyclodextrins would absorb the products of the hydrolysis reaction of lipid substrates, would make it possible to regulate the kinetics of fat breakdown, would absorb unpleasant odor molecules due to the hydrophobic cavity, and would reduce the amount of water and electricity consumed per 1 wash of linen.

[0027] In patent application EP1075509A1 (W09957254A1) [THE PROCTER & GAMBLE COMPANY] publ. On April 30, 1999, the use of a composition for the preparation of laundry detergents containing a modified transferase was disclosed. The modified enzyme contains a catalytically active transferase domain linked to the amino acid sequence of the cellulose binding domain to effectively remove stains and improve the washing process. The component can be used in conjunction with a system of surfactants, enzymes (protease, cellulase, lipase, amylase and / or a mixture of these enzymes), bleaching agents, fillers, releasing agents, optical brighteners, fabric softeners, dispersing agents, dye leaching inhibitors from fabrics , abrasives, bactericidal substances, fragrances. The composition based on the modified transferase is aimed at removing body sebum and contaminants based on plant fibers, sugars, amylose, which are difficult to remove during washing, especially at low temperatures. Thus, a need has been described for a composition for removing soil, smoothing and softening laundry, controlling static, maintaining color, and improving fabric durability.

[0028] The composition described above from patent application EP1075509A1 (W09957254A1) is designed to specifically cleave polysaccharide substrates, in particular glycosidic dimers, oligomers and/or polymers from starch, xyloglycans, cyclodextrins, sucrose and maltose. Cyclodextrins do not increase the effectiveness of the composition, since they are substrates for the enzyme that breaks them down. The enzyme lipase is described as a possible additional enzyme in this compositions along with protease, cellulase, amylase. In the examples given, the content of lipase varies from 0.002 to 0.01%, which does not completely break down lipid contaminants in short laundry programs (up to 30 minutes).

The composition does not have the ability to specifically absorb unpleasant odors that are fixed by sebum and lipid deposits, but only partially removes them during washing and drying clothes. The use of a lipase enzyme with high activity and an increase in its content would increase the efficiency of removing fatty contaminants and eliminating odors due to the presence of specific esters responsible for unpleasant odors, and non-granular cyclodextrins would absorb the products of the hydrolysis reaction of lipid substrates, would allow regulating the kinetics of fat breakdown, would absorb unpleasant odor molecules due to the hydrophobic cavity and would reduce the amount of water and electricity consumed per wash.

[0029] In contrast to patent applications W01990010687A1, WO01/18163A1 and EP1075509A1 (W09957254A1), the authors of the present invention investigated the effectiveness of a biodegradable composition containing biotechnologically produced lipase and b-cyclodextrins, and found a synergistic effect for the regulation of enzyme kinetics and rapid cleavage lipid contaminants and deposits from various surfaces (wood, metal, enamel, polymer, ceramic, faience, etc.), additional emulsification of fats and fatty acids, absorption of unpleasant odors from various surfaces, ensuring the stability of liquid detergents with aromatic components during storage. The combination is safe on the skin of the hands and maintains dermatological comfort of the skin, which allows to reduce the content of anionic surfactants that cause an increase in the sensitivity of the skin of the hands, and does not cause an irritant effect. Due to the synergistic effect of lipase and b-cyclodextrins, it becomes possible to reduce the content of anionic surfactants or combinations with them by 30% or more. The addition of b-cyclodextrins makes it possible to reduce the reaction temperature and reduce the peroxidation of triglycerides of unsaturated fatty acids, which are contained in soybean, corn, linseed, olive and other edible oils. During oxidation, oxides and peroxides of acids are formed, which have a specific smell of rancidity, give a yellowish color and cause clouding of dishes made of polymeric materials. [0030] The technical result of the innovative complex is the effective removal of fatty contaminants due to the regulation of the kinetics of lipid cleavage contaminants, eliminate unpleasant odors and maintain the thermal stability of samples. The complex is active in a wide pH range, in particular 5.0-10.0 units, and a wide temperature range, in particular from +5 to +60°C, which expands the scope of application in detergents and cleaners.

[0031 ] The combination of lipase and b-cyclodextrins has been found to increase the removal efficiency of complex protein-lipid soils on various surfaces, in particular metal, polymer, glass and wood surfaces, as well as increase the cleaning efficiency of products by more than 35% on various surfaces while maintaining the content of surfactants based on components of natural origin. In this case, lipase and b-cyclodextrins are understood as raw materials containing the corresponding active components, as well as technical impurities that could be formed during the production of the target raw material.

[0032] Combining components allows for synergistic action and retention of efficiency at a lower percentage of input of individual components. Lipase, obtained by modern methods of biotechnology without the use of GMOs, is an active enzyme that breaks down insoluble lipid substrates with triglyceride ester bonds at the water-oil or oil-water interface. b-Cyclodextrins act as coactivators (colipases) by binding to the terminal domain of lipase and forming a clathrate shell from water molecules, contributing to a change in the conformation of the active center of the lipase and reducing the activation energy of the process (AE _a ), which facilitates the enzymatic reaction. The lipase enzyme lowers the activation energy by increasing the number of activated fatty acid triglyceride and glycerol molecules, which become reactive at a lower energy level, which lowers the energy barrier to further enzymatic reaction. Additionally, cyclodextrins, due to the hydrophilic surface due to free hydroxyl groups -OH, specifically orient lipase substrates at the water-oil or oil-water interfaces, increasing the availability of the ester bond for enzymatic hydrolysis. Since the enzymatic reaction is an equilibrium one, in order to shift the equilibrium of the reaction towards the formation of hydrolysis products, it is necessary to bind and remove free fatty acids from the system. In particular, b-cyclodextrins bind the reaction products due to their emulsifying properties, shifting the reaction equilibrium towards the breakdown of lipid substrates in the system. [0033] An additional property is the neutralization of odorous aromatic substances represented by various classes of organic substances: aldehydes and ketones, terpenes, amines, indole, sulfur-containing components (mercaptans), organic acids and their esters, phenols and cresols. Since a person is more sensitive to unpleasant odors, the neutralization of odorous substances becomes the main tool for managing the emotional state. In such cases, cleaning with conventional household chemicals does not provide effective odor removal, so there is a need for a special combination to control and eliminate odors in addition to regulating the kinetics of hydrolysis of lipid contaminants.

[0034] An innovative complex, including lipase and b-cycle o dextrins, is aimed at effective cleansing and removal of fatty contaminants by regulating the kinetics of the cleavage reaction of lipid substrates and increasing the washing efficiency of household chemicals, as well as neutralizing unpleasant odors. The complex based on natural and biodegradable components is effective in the pH range of 5.0-11.0 in the presence of various synthetic and natural components, which allows it to be used in a wide range of household chemicals for cleaning various surfaces, such as laundry detergents, washing dishes, floors, glasses, universal cleaners. The components target lipid and complex contaminants on various surfaces, and also bind the released metabolic products with an unpleasant odor for humans. After applying the composition, the cleanliness of surfaces and a pleasant aroma are preserved longer. Thus, the combined use of the components in the claimed composition in one product leads to an increase in the kinetics of the enzymatic reaction of the breakdown of complex household soiling by lipids due to stabilization, orientation of substrates and an increase in lipase activity due to b-cyclodextrins, which allows for rapid cleansing even under cold water conditions. A distinctive feature is that the components in the claimed concentrations act only on complex contaminants and do not violate the consumer appearance of most surfaces, in particular metal, wood, polymer and enamel. The composition does not contain aggressive inorganic substances and organic solvents, occlusive film formers and alcohols, so it is possible to use detergents and cleaners with this composition on a regular basis without harm to the skin of human hands. The combined use of these components exhibits a synergistic effect that provides complete care. behind various surfaces in one household chemical product for daily use.

[0035] Thus, the invention generally relates to a composition and its use, allowing to achieve such technical results as the effective regulation of the kinetics of enzymatic degradation of lipids while effectively neutralizing unpleasant odors on various types of surfaces, including especially metal, polymer, enamel, glass and wooden, with long-term cleanliness and pleasant aroma, which are not achieved or insufficiently achieved by modern commercially available means in this field of technology.

SUMMARY OF THE INVENTION

[0036] In one aspect, the invention relates to a composition for use in a household product, consisting of lipase and b-cyclodextrin, where the weight ratio of lipase and b-cyclodextrin is (0.0025-0.25):(0. 1-1) respectively.

[0037] The composition may differ in that the amount of lipase in the specified weight ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0, 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25.

[0038] The composition may differ in that the amount of b-cyclodextrin in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0 .2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8 , 0.85, 0.9, 0.95 or 1.

[0039] The composition may be characterized in that said lipase is in an aqueous glycerol solution.

[0040] The composition may be characterized in that said aqueous glycerol lipase solution is a commercial product Lipex® Evity® 200 L. Lipex® Evity® 200 L is available, in particular from Novozymes and can be identified, for example, by

<https://biosolutions.novozymes.com/en/laundry/products/lipexr-evityr-200-l>.

[0041] The composition may be characterized in that said aqueous glycerol lipase solution is a commercially available Lipex® Evity® 200 L modified with additional glycerol. [0042] The composition may differ in that the composition contains Lipex® Evity® 200

L, modified with an additional amount of glycerol, and b-cycle about dextrin, where the content of Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. to the mass of the composition.

[0043] The composition may differ in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerol, the mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is (0.1-1): (0 .5-1.5), respectively.

[0044] The composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 .3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9 , 0.95 or 1.

[0045] The composition may differ in that the amount of additional glycerol introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.5, 0.55, 0.6, 0.65, 0, 7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5.

[0046] The composition may differ in that it additionally contains decylglucoside. [0047] The composition may differ in that said decyl glucoside is a commercially available NaturalAPG HG0814CM product. NaturalAPG HG0814CM is available in particular from Hugo and can be identified for example at <https://www.hugochem.net/alkyl-polyglucoside/apg-0814/decyl-glucoside-apg-2000up.html>.

[0048] The composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14).

[0049] The composition may differ in that the amount of Lipex® Evity® 200 L in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5, or 0.6.

[0050] The composition may differ in that the amount of NaturalAPG HG0814CM in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, or fourteen.

[0051] The composition may differ in that the composition contains commercially available product Lipex® Evity® 200 L modified with additional glycerol, b-cyclodextrin and commercially available product NaturalAPG HG0814CM, where the content of commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. to the mass of the composition.

[0052] The composition may be characterized in that the activity of said lipase is at least 40 LU/r or 40 LU/ml at pH 7.0.

[0053] The composition may differ in that said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pretreatment stain remover, laundry gel and fabric softener.

[0054] In another aspect, the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention.

[0055] In another aspect, the invention relates to a floor cleaner, and/or a toilet bowl, and/or a sink, and/or a bathtub, and/or glasses, containing 0.0525-1% wt. compositions according to the invention.

[0056] In another aspect, the invention relates to a universal cleaner for cleaning surfaces containing 0.0525-1% wt. compositions according to the invention. [0057] In another aspect, the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention.

[0058] The tool for cleaning pipes may differ in that the specified tool contains 0, 0525-1, 5% wt. the specified composition, preferably 0,0525-1%wt. specified composition.

[0059] In another aspect, the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention.

[0060] The laundry detergent may be characterized in that said detergent is selected from a delicate laundry detergent and a baby laundry detergent. [0061] The laundry detergent may be characterized in that said agent is a powdered laundry detergent.

[0062] In another aspect, the invention relates to the use of a composition of the invention for regulating the kinetics of enzymatic lipid breakdown and neutralizing unpleasant odors on various surfaces, maintaining long-term cleanliness and pleasant aroma. [0063] The application may differ in that said surface is selected from metal, polymer, enamel, glass or wood.

[0064] In another aspect, the invention relates to a method for preparing a composition according to the invention, comprising preliminary adding a solution of lipase to a dispersion of b-cyclodextrin, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes.

[0065] the Invention will be further disclosed in detail in particular cases of its implementation and illustrated by examples of implementation.

DETAILED DESCRIPTION OF THE INVENTION

[0066] In the tool according to the invention, auxiliary acceptable substances can be selected from the following categories of components.

[0067] Anionic surfactants:

[0068] Salts of higher carboxylic acids with the general formula: R1-C02X1, where R1 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI is an alkali and / or alkaline earth metal, ammonium, alkylammonium cation, alkanolammonium, glucoammonium, basic amino acid;

[0069] Alkyl polyethylene glycol sulfate with the general formula: R2-0(-CH2-CH2-0) nlS03X2, where nl can take values from 1 to 10, and denotes the number of polyethylene glycol groups, R2 is an alkyl and / or alkenyl group with a long hydrocarbon chains from 6 to 22 carbon atoms, and X2 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium; [0070] Alkyl sulfate with the general formula R3-0S03X3, where R3 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, and X3 is an alkali and / or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation ;

[0071] A higher fatty acid amide salt of methylglycine with the general formula R4-C(0)-N(-CH3)-CH2-C02X4, where R4 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms, a X4 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation; [0072] Alkyl polyethylene glycol carboxylate with the general formula: R5-0(-CH2-CH2-0-)p2CH2-C02X5, where p2 can take values from 1 to 15, and denotes the number of polyethylene glycol groups, R5 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, and X5 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0073] Disubstituted salt of 2-sulfocarboxylic acid with the general formula: R6-CH(-

S03X6)-C02X6, where R6 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 4 to 20 carbon atoms, and X6 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;

[0074] A mono or disubstituted higher carboxylic acid amide salt of glutamic acid with the general formula: R7-C(0)-NH-CH(-CH2-CH2-C02X7)-C02X7, where R7 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X7 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium or hydrogen cation;

[0075] A higher fatty acid amide salt of glycine with the general formula: R8-C(0)-NH-CH2-C02X8, where R8 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms and X8 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;

[0076] A higher fatty acid amide salt of alanine with the general formula: R9-C(0)-NH-CH(-CH3)-C02X9, where R9 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , and X9 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium; [0077] A higher fatty acid amide salt of 2-aminomethylethanesulfonic acid with the general formula: R10-C(0)-N(-CH3)-CH2-CH2-S03X10, where R10 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X 10 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;

[0078] Alkyl polyglucoside hydroxypropylsulfonate with the general formula: Rll-O- [G]pl-0-CH2-CH(-0H)-CH2-S03Xl 1 where R11 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, pi can take values from 1 to 4, and XI 1 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;

[0079] Alkyl polyglucoside carboxylate with the general formula: R12-0-[G]p2-0-CH2-C02X12, where R12 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p2 - can take values from 1 to 4, and XI 2 - cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium; [0080] Higher fatty acid amide salt of threonine with the general formula: R13-C(0)-

NH-CH (-CH (-0H) -CH3) -C02X13, where R13 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI 3 is an alkali and / or alkaline earth metal, ammonium cation, alkylammonium, alkanolammonium, glucoammonium;

[0081] An amide salt of a higher fatty acid and an amino acid derived from the hydrolysis of proteins from plant materials, with the general formula: R14-C(0)-AAX14, where R14 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , AA is an amino acid or peptide obtained by hydrolysis of vegetable protein (possible protein sources: apple, soybean, wheat, cotton, etc.), and XI 4 is an alkali and / or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation.

[0082] Amphoteric surfactants:

[0083] Disubstituted salt of acylamphodiacetate with the general formula: R15-C(0)-NH-CH2-CH2-N(-CH2-C02X15)-CH2-CH2-0-CH2-C02X15, where R15 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI 5 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation;

[0084] Acylamphoacetate salt with the general formula: R16-C(0)-NH-CH2-CH2-N(-CH2-C02X16)-CH2-CH2-0H, where R16 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI 6 - cation of alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium; [0085] An alkylamphoacetate salt with the general formula: R17-C(=N-CH2-CH2-N((-CH2-CH2-0H)-CH2-C02X17)-), where R17 is an alkyl and/or alkenyl group with a long hydrocarbon chains from 5 to 21 carbon atoms, and XI 7 is an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium cation; [0086] Acylamidoalkyl betaine with the general formula: R18-C(0)-NH-R19-N(-CH3)2)-CH2-CO2, where R18 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , R19 - alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms;

[0087] Acylamidoalkylhydroxysultaine with the general formula: R20-C(O)-NH-R21-N(-CH3)2-CH2-CH(-0H)-CH2-S03, where R20 is an alkyl and/or alkenyl group with a long hydrocarbon chains from 5 to 21 carbon atoms, R21 is an alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms; [0088] Acylamidoalkylamine oxide with the general formula: R22-C(0)-NH-R23-N(-CH3)2-0, where R22 is an alkyl and/or alkenyl group with a hydrocarbon chain length of 5 to

21 carbon atoms, R23 is an alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms;

[0089] Alkyl betaine with the general formula: R24-N(-CH3)2)-CH2-C02, where R24 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms; [0090] Alkylhydroxysultaine with the general formula: R25-N(-CH3)2-CH2-CH(-OH)-CH2-SO3, where R25 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;

[0091] Alkylsultaine with the general formula: R26-N(-CH3)2-CH2-CH2-CH2-SO3, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;

[0092] Alkylamine oxide with the general formula: R27-N(-CH3)2-0, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms.

[0093] Nonionic surfactants:

[0094] Alkyl glucoside with the general formula: R28-0-[G]p3, where R28 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 4 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p3 - can take values from 1 to 4;

[0095] Alkyl polyethylene glycol with the general formula: R29-0(-CH2-CH2-0-)n3H, where n3 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, R29 is an alkyl and / or alkenyl group with a long hydrocarbon chain 6 to 22 carbon atoms;

[0096] Alkyl polyethylene/propylene glycol with the general formula: R30-O(-CH2-CH2-O-)n4(-CH(-CH3)-CH2-0-)n5H, where n4 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, n5 can take values from 2 to 20, and denotes the number of polypropylene glycol groups, R30 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms;

[0097] Dialkyl polyethylene glycol with the general formula: R31-0(-CH2-CH2-0-)n6R32, where pb can take values from 2 to 20, and denotes the number of polyethylene glycol groups, R31 is an alkyl and / or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, R32 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 1 to 12 carbon atoms;

[0098] Dialkylpolyethylene/propylene glycol with the general formula: R33-0(-CH2-CH2-0-)n7(-CH(-CH3)-CH2-0-)n8-R34, where n7 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, n8 can take values from 2 to 20, and denotes the number of polypropylene glycol groups, R33 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, R34 is an alkyl and/or alkenyl group with a long hydrocarbon chain chains from 1 to 12 carbon atoms.

[0099] Polysaccharide dispersion medium/solvent:

[0100] An organic alcohol with the general formula: R35(-OH)sl, where R35 is an alkyl group with a long hydrocarbon chain from 3 to 12 carbon atoms, S1 - can take values from 1 to 12, and denotes the number of hydroxyl groups arranged in hydrocarbon radical in an arbitrary order relative to each other;

[0101] Alkyl polypropylene glycol with the general formula: H(-CH(-CH3)-CH2-0-)n9R36, where n9 can take values from 2 to 10, and denotes the number of polypropylene glycol groups, R36 is an alkyl group with a long hydrocarbon chain from 1 up to 10 carbon atoms.

[0102] pH Regulators:

[0103] Organic acids with the general formula: R37(-OH)s2(-COOH)ml, where R37 is an alkyl group with a long hydrocarbon chain from 1 to 12 carbon atoms, S2 - can take values from 1 to 12, and denotes a number hydroxyl groups located in the hydrocarbon radical, in an arbitrary order relative to each other, Ml - can take values from 1 to 4, and denotes the number of carboxyl groups located in the hydrocarbon radical, in an arbitrary order relative to each other;

[0104] Solutions of alkali or alkaline earth metal hydroxides, ammonia, primary and tertiary alkylamines, primary and tertiary alkanolamines, primary and tertiary glucamines, basic amino acids, disodium citric acid, trisodium citric acid.

[0105] Chelating agent:

[0106] Methylglycine diacetic acid trisodium salt, glutamine diacetic acid tetrasodium salt, ethylenediamine-(M,1\[)-disuccinate trisodium salt; [0107] Organic acids, as well as salts of alkali metals, ammonium, alkylammonium, alkanolammonium, glucoammonium, corresponding to these acids: citric acid, malic acid, tartaric acid, glutaric acid, adipic acid, glucuronic acid, galacturonic acid, galactaric acid, gluconic acid , phytic acid, polytaconic acid, polyacrylic acid, polymethacrylic acid, copolymer of acrylic and maleic acids, as well as the same organic acids with the general formula R38(-OH)s3(-COOH)m2, where R38 is an alkyl group with a long hydrocarbon chain from 1 to 12 carbon atoms, S3 - can take values from 1 to 12, and denotes the number of hydroxyl groups, located in the hydrocarbon radical, in an arbitrary order relative to each other, M2 - can take values from 1 to 4, and denotes the number of carboxyl groups located in the hydrocarbon radical, in an arbitrary order relative to each other.

[0108] Pollution re-deposition inhibitors:

[0109] Polysaccharide derivatives: carboxymethyl polysaccharide sodium salt, hydroxyalkyl polysaccharide, alkyl polysaccharide;

[IT] Polyvinylpyrrolidone;

[0111] Water-soluble salts of polyacrylic acid, polymethacrylic acid, acrylic/methacrylic copolymer, and maleic acid.

[0112] Defoamers:

[0113] Higher carboxylic acids with the general formula: R39-C02H, where R39 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms; [0114] Higher carboxylic alcohols with the general formula: R40-COH, where R40 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms; [0115] Ethers of higher carboxylic alcohols with the general formula: R41-0-R42, where R41, R42 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 4 to 22 carbon atoms;

[0116] Bisamides of alkyldiamines and higher carboxylic acids: with the general formula: R43-C(0)-NH-R44-NH-C(0)-R45, where R43, R45 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, a R44 is an alkyl radical with a hydrocarbon chain length of 1 to 12 carbon atoms;

[0117] Preservatives:

[0118] Organic acids and salts of alkali and alkaline earth metals, ammonium, alkylammonium, alkanolammonium, glucoammonium corresponding to these acids: benzoic acid, sorbic acid, 4-methoxybenzoic acid, salicylic acid, undecylenic acid;

[0119] Organic alcohols and phenols: phenoxyethanol, benzyl alcohol, caprylyl glycol, ethylhexylglycerin, phenethyl alcohol, 3-methyl-4-isopropylphenol, 2,4-dichlorobenzyl alcohol;

[0120] Broad spectrum biocides: benzisothiazolinone, dodecyldipropylene triamine; [0121] Fungicides: sodium pyrithione, climbazole.

[0122] Enzymes: protease, amylase, pectate lyase, mannanase, cellulase, amine oxidase, nuclease, ferruloyl esterase, gluconase, tannase and other commercially available enzymes that are used in laundry, dishwashing, flooring, glass, all-purpose cleaners .

[0123] Oxygen based bleaches: hydrogen peroxide, calcium peroxide, carbamide peroxide, e-phthalimidoperoxycaproic acid, and other commercially available ingredients.

[0124] In general, the present invention can be characterized by the following features.

[0125] In one aspect, the invention relates to a composition for use in a household product, consisting of lipase and b-cyclodextrin, where the weight ratio of lipase and b-cyclodextrin is (0.0025-0.25):(0.1 -1) respectively.

[0126] The composition may differ in that the amount of lipase in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0, 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25. The composition may differ in that the amount of b-cyclodextrin in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0.2, 0 .25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85 , 0.9, 0.95 or 1.

[0127] The composition may be characterized in that said lipase is in an aqueous glycerol solution.

[0128] The composition may be characterized in that said aqueous glycerin lipase solution is a commercially available product Lipex® Evity® 200 L. The composition may be characterized in that said aqueous glycerin lipase solution is a commercially available product Lipex® Evity® 200 L modified with additional glycerin. The composition may differ in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerol, and b-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. to the mass of the composition. The composition may differ in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerol, the mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is (0.1-1): (0.5- 1.5) respectively. [0129] The composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 .3, 0.35, 0.4, 0.45, 0.5, 0.55,

0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, or 1. The composition may differ in that the amount of additional glycerol introduced for modification in the indicated mass ratio Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0 .95, 1, 1.1,

1.2, 1.3, 1.4 or 1.5.

[0130] The composition may be characterized in that it additionally contains decyl glucoside. The composition may differ in that said decyl glucoside is a commercially available NaturalAPG HG0814CM product. The composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14). The composition may differ in that the amount of Lipex® Evity® 200 L in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0.6. The composition may differ in that the amount of NaturalAPG HG0814CM in the indicated weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5 or 14. Composition may differ in that the composition contains a commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerol, b-cyclodextrin and a commercially available product NaturalAPG HG0814CM, where the content of a commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. to the mass of the composition.

[0131] The composition may be characterized in that the activity of said lipase is at least 40 LU/r or 40 LU/ml at pH 7.0.

[0132] The composition may differ in that said household cleaner is selected from dishwashing detergent, floor cleaner and/or toilet bowl and/or sink and/or tub and/or glass, pipe cleaner, Laundry detergents, including delicate laundry detergents or baby laundry detergents, fabric softener, laundry pre-treatment and laundry stain remover, laundry gel and fabric softener.

[0133] In another aspect, the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention. [0134] In another aspect, the invention relates to a floor cleaner, and/or a toilet bowl, and/or a sink, and/or a bathtub, and/or glasses, containing 0.0525-1% wt. compositions according to the invention.

[0135] In another aspect, the invention relates to a universal cleaner for cleaning surfaces containing 0.0525-1% wt. compositions according to the invention. [0136] In another aspect, the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention. Means for cleaning pipes may differ in that the specified tool contains 0.0525-1.5% wt. the specified composition, preferably 0,0525 - 1% wt. specified composition.

[0137] In another aspect, the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention. The laundry detergent may differ in that said detergent is selected from a delicate laundry detergent and a baby laundry detergent. The laundry detergent may be characterized in that said agent is a powdered laundry detergent.

[0138] In another aspect, the invention relates to the use of the composition of the invention for regulating the kinetics of enzymatic lipid breakdown and neutralizing unpleasant odors on various surfaces, maintaining long-term cleanliness and pleasant aroma. The application may differ in that said surface is selected from metal, polymer, enamelled, glass or wood.

[0139] In another aspect, the invention relates to a method for preparing a composition according to the invention, including the preliminary introduction of a solution of lipase into a dispersion of b-cyclodextrin, in a solvent, for example, water, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes. This allows a homogeneous solution to be obtained.

EXPERIMENTAL PART

[0140] The examples included in the present description are not limiting the claimed invention and are provided only for the purpose of illustrating and confirming the achievement of the expected technical results. These examples are one of many experimental data obtained by the inventors, which confirm the effectiveness of the means that are within the scope of the invention. [0141] Were conducted preclinical studies to evaluate the effectiveness of the composition according to the invention.

Example 1

[0142] A laboratory study was conducted on the washing effectiveness of active ingredients, namely lipase and b-cyclodextrins, in the composition of detergents on the same basis.

[0143] A mixture consisting of purified water, anionic surfactants 5-15%, nonionic surfactants based on glycosides <5%, glycerin, cotton extract, tetrasodium diacetate glutamate, monohydrate citric acid, sodium hydroxide, a preservative, and an aqueous solution of citric acid and silver citrate (Table N°1).

[0144] The dishwashing detergent base was prepared as follows: the solubilizer glycerin and the chelating agent glutamate diacetate tetrasodium salt were mixed with purified water while stirring in an ordinary mixer until the mixture was homogeneous. Pre-homogenized anionic surfactants with a sufficient amount of water purified by heating 40-60°C. Next, non-ionic surfactants based on glycosides and an anionic surfactant solution were added. Cotton extract and an aqueous solution of citric acid and silver citrate were also added. At the very end, an organic acid was added to adjust the pH, a preservative to maintain microbiological stability throughout the shelf life. The ingredients were mixed until a homogeneous clear solution was obtained. After mixing, the pH of the product was measured and adjusted by adding sodium hydroxide to the desired value.

Table N° 1. Composition of the base of household chemicals

[0145] The test procedure is based on OST 6-15-1662-90 "Household cleaning products". The method for determining the washing ability for various surfaces is that the gravimetric method determined the mass of removed artificial oil-fat contamination for a certain time with the test agent in relation to the comparison agent. Glass plates were chosen as surfaces. The glass plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and rubbed with ethyl alcohol. After preparation, the plates were weighed to determine the baseline. Next, a complex oil-fat pollutant was prepared, consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1:4.9:1.0 :5,1:7,3:2,1:4,5:20 respectively. Weighed lubricants and lanolin were mixed at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled, and egg yolk and other components were added. The contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to an oven and calcined at 220±5°C for 8 minutes. Then cooled and weighed. The samples were used in pure form. As a means of comparison, a standard household chemical product with a similar quantitative composition was used.

[0146] For testing, each plate was placed in a jar with a lid, a nylon fabric was placed on top and 1 jar with a plate was filled with a clean sample of the test agent, another 1 jar with plates was filled with 40 ml of a reference agent to maintain the ratio of water: lipophilic contaminant (fat , oils, glycerides) such as 9:1. The jars were placed in a liquid shaking machine and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.

[0147] According to the established data, the mass fraction of the removed dirt was calculated. The mass fraction of the removed pollutant was calculated using the following formula:

A is the mass of a piece of a plate with a pollutant before washing with a means, g;

B is the mass of a piece of a plate with a contaminant on the plates after washing with the agent, g.

B is the mass of a clean plate, g.

[0148] The parameter for the overall assessment of the washing efficiency and the kinetics of the breakdown of lipid contaminants in the composition of the product was the calculation of % washing efficiency, determined by the laboratory assistant based on the results of the study. The weight fraction of contaminant removed measures the cleaning performance against difficult lipid-based soils when the composition is used daily in a dishwashing detergent. The general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the dishwashing detergent and various surfaces.

[0149] For quantitative data, the group arithmetic mean (M), standard deviation (SD), and standard error of the mean (SEM) were calculated. The obtained data were processed using the MS Excel program. The probability of differences in mean indicators at different points in time was determined using Student's t-test with normal distribution in independent and dependent samples. Differences were considered significant at a significance level of p<0.05.

Results.

[0150] Based on the results of evaluating the washing efficiency of the samples, it was found that the test composition in the composition of the dishwashing detergent has a pronounced washing effect on complex lipid-protein contaminants when compared with a control agent that does not contain components of the claimed composition.

[0151] At the end of the study, there were marked changes in the estimated detergency index. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, the removal efficiency of the complex lipid-protein pollutant increased by 53.6% on average. The control version did not reach 100%, indicating that the combination of surfactants was not effective enough to remove lipid-protein contaminants (Table III).

Table N° 2. Evaluation of the washing efficiency of the samples

[0152] Complex lipid-protein contaminants are difficult to remove from various surfaces due to fixation and insufficient action of surfactants.

[0153] The base of the dishwashing detergent composition will gently clean dirt from the surfaces of dishes (glass, metal, polymer, ceramic) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in relation to complex lipid-protein contaminants, including obsolete. The study of a dishwashing detergent with the claimed composition has led to a pronounced synergistic effect in relation to soils that are difficult to remove using conventional surfactant-based dishwashing detergents. The composition makes it possible to replace synthetic surfactants with surfactants of natural origin without losing the effectiveness of the product, which reduces the burden on the environment, maintains an approach to human health and provides a high percentage of natural ingredients in the composition of household chemicals for consumers.

[0154] Since the composition contains natural biotechnology-derived lipase and natural b-cyclodextrins, a high % removal of complex contaminants with low labor and time savings will be achieved with a single application. Combining the components in the composition makes it possible to regulate the kinetics of the enzymatic cleavage of lipid substrates that fix complex contaminants. Lipase provides targeted cleavage of lipid contaminants in various phase systems, and cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, providing changes in the conformation of the protein domain with the active center and the correct orientation of substrates. Additionally, cyclodextrins bind released cleavage products that have an unpleasant smell, which allows you to neutralize odorous aromatic molecules and control the atmosphere in the room for the psychological comfort of a person.

[0155] Thus, the combination of components makes it possible to achieve a synergistic effect in relation to complex lipid-protein contaminants due to the targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows regular use as part of household chemicals for various surfaces.

Example 2

[0156] A laboratory study was conducted on the washing effectiveness of the active components, namely lipase and b-cyclodextrins. Purified water and glycerol for solubilization of b-cyclodextrins were used as a base for the inclusion of the complex components.

[0157] The test method is based on OST 6-15-1662-90 "Household cleaning products". The method for determining the washing ability for various surfaces is that the gravimetric method was used to determine the mass of removed artificial oil-fat contamination for a certain time by the test sample in relation to the reference sample. Metal and plastic plates were chosen as surfaces. 8 metal and 8 plastic plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and rubbed with ethyl alcohol. After preparation, the plates were weighed to determine the baseline. Next, a complex oil-fat pollutant was prepared, consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1:4.9:1.0 :5,1:7,3:2,1:4,5:20 respectively. Weighed lubricants and lanolin were mixed at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled, and egg yolk and other components were added. The contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to an oven and calcined at 220±5°C for 8 minutes. Then cooled and weighed. The samples were used in pure form. As a means of comparison, distilled water was used, not containing the claimed composition.

[0158] For testing, each plate was placed in a jar with a lid, a nylon fabric was placed on top and 1 jar with a plate was filled with a test sample, another 1 jar with plates was filled with 40 ml of a reference sample to the ratio of water:lipophilic contaminant (fat, oils, glycerides) was 9:1. The jars were placed in a liquid shaking machine and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.

[0159] According to the established data, the mass fraction of the removed dirt was calculated. The mass fraction of the removed pollutant was calculated using the following formula:

where

A is the mass of a piece of plate with a contaminant before washing with a sample, g;

B is the mass of a piece of a plate with a contaminant on the plates after washing with a sample, g.

B is the mass of a clean plate, g.

[0160] The parameter for the overall assessment of the washing efficiency and the kinetics of the breakdown of lipid contaminants in the composition of the product was the calculation of % washing efficiency, determined by the laboratory assistant based on the results of the study. The indicator of the mass fraction of the removed pollutant allows you to evaluate the washing effectiveness in relation to complex lipid-based contaminants (oils and fats with fixing additives) during daily use of the composition in the composition of a dishwashing detergent. The general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the dishwashing detergent and various surfaces.

[0161] For quantitative data, the group arithmetic mean (M), standard deviation (SD), and standard error of the mean (SEM) were calculated. The obtained data were processed using the MS Excel program. The probability of differences in mean indicators at different points in time was determined using Student's t-test with normal distribution in independent and dependent samples. Differences were considered significant at a significance level of p<0.05.

Results.

[0162] Based on the results of evaluating the washing efficiency of the samples, it was found that the test composition has a pronounced washing effect on complex lipid-protein contaminants on various surfaces when compared with a control sample that does not contain components of the claimed composition.

[0163] At the end of the study, there were marked changes in the estimated indicator of washing efficiency on the hydrophilic stainless steel metal surface. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, an increase in the content of the components resulted in a statistically significant (p<0.05) increase in the removal of contaminant by the baseless formulation with surfactants. First, there is a logarithmic increase in efficiency along the curve, and then an exit to the stationary phase, when an increase in the content of components does not affect the increase in efficiency due to a shift in the equilibrium in the enzymatic reaction (Table N ° 3). The rapid accumulation of reaction products leads to a chemical reaction constant K _pB n and a shift in the equilibrium towards the reverse reaction due to the concentration gradient, as well as a change in pH in the system.

[0164] The change in washing efficiency on a hydrophobic polymeric surface made of plastic is similar to a metal surface, however, contaminants are removed better even with a minimum percentage of composition input due to the physicochemical characteristics of the surface. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, an increase in the content of the components led to a statistically significant (p<0.05) increase in the removal of the pollutant by the composition without a base with surfactants. First, there is a logarithmic increase in efficiency along the curve, and then an exit to the stationary phase, when an increase in the content of components does not affect the increase in efficiency due to a shift in the equilibrium in the enzymatic reaction (Table N°4). [0165] The control version did not show effective removal of contaminants, which indicates a targeted effect and high activity of the composition in a certain concentration range.

Table Ns 2. Evaluation of washing efficiency on a metal surface

Table 3. Evaluation of washing efficiency on a polymer surface

[0166] Complex lipid-protein soils are difficult to remove from various surfaces in the home due to fixation and insufficient action of surfactants from household chemicals, despite their solubilizing properties.

[0167] The base of the dishwashing detergent composition will gently clean dirt from the surfaces of dishes (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in relation to complex lipid-protein contaminants , incl. obsolete. The study of the washing effectiveness of the composition showed a pronounced synergistic effect with respect to dirt that is difficult to remove using conventional dishwashing detergents based on natural surfactants. The composition makes it possible to replace synthetic surfactants with surfactants of natural origin or to reduce their content equivalently without losing the effectiveness of the product, which allows reducing the burden on the environment, maintaining an approach to human health and providing a high percentage of natural ingredients in the composition of household chemicals for consumers.

[0168] Since the composition contains biotechnology-derived lipase and natural b-cyclodextrins, a high % removal of difficult contaminants with low labor and time savings will be achieved with a single application. The combination of components in the composition allows you to control the kinetics of the enzymatic cleavage of lipid substrates that fix complex pollution. Lipase provides targeted cleavage of lipid contaminants in various phase systems, and cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, providing changes in the conformation of the protein domain with the active center and the correct orientation of substrates. In addition, cyclodextrins bind the released breakdown products with an unpleasant odor, which makes it possible to neutralize odorous aromatic molecules and control the atmosphere in the room for the psychological comfort of a person.

[0169] Since the kinetics of the reaction is subject to the influence of the reaction products, in particular, the non-competitive effect of the released fatty acids on the allosteric centers of the lipase, the b-cyclodextrins act as activity stabilizers and bind the reaction products from the system. The joint use of components retains high activity at different % input of the composition, depending on the type of household chemicals and its composition.

[0170] Thus, the combination of components makes it possible to achieve a synergistic effect on complex lipid-protein contaminants due to the targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows regular use as part of household chemicals for various surfaces.

Example 3

[0171] A laboratory study was conducted on the deodorizing effectiveness of active components, namely lipase and b-cyclodextrins, and household chemicals with these substances. Purified water and glycerin for the solubilization of b-cyclodextrins, as well as the mixture given in Table N°l, were used as a base for the inclusion of the components of the complex.

[0172] The test methodology is based on Deodorizing Ability of Houttuynia cordata Thunb. (Dokudami) for Masking Garlic Odar - Hiromi Ikeura and allows you to evaluate the ability of the components to neutralize unpleasant odors by organoleptic method.

[0173] Organic compounds of the group of thioethers R _I -SR.2 AND esters R3-CO-OR4 from the fruits of garlic Allium sativum, obtained by ultrasonic extraction with alcohol 96% and filtration through a Schott funnel, were selected as unpleasant odors. Sterile medical containers made of polymeric material were used as auxiliary materials. Using a pipette, 0.5 g of an alcohol extract was applied strictly to the bottom of each of the polymer containers and placed in preheated oven (set temperature 60°C) for 15-

30 minutes. Next, 1.0 g of purified water was poured onto the bottom of the containers and left for 10 minutes. After that, 0.8-1.0 g of the tested household chemicals were applied to the bottom of the glasses and mixed for 3-5 minutes using a vibrator. After 3 minutes, each of the containers is washed with 1 liter of warm running water of medium hardness to completely remove the liquid, and then dried at room temperature. The deodorizing effect was determined by the organoleptic method and was evaluated according to the developed 5-point scale, where 0 points - does not deodorize at all, and 5 points - completely deodorizes.

[0174] Samples of household chemicals were used in pure form. As a means of comparison, distilled water was used, not containing the claimed composition, and a water-soluble zinc salt, which has a pronounced deodorizing effect due to the chemical structure of zinc ions Zn ²⁺ . To evaluate the result, a sensory group of 5 people was involved, since individual variability in the intensity of the aroma could be observed. For quantitative data, the average score obtained from the results of the study was calculated.

Results.

[0175] Based on the results of the study, it was found that the test composition in the composition of household chemicals has a pronounced deodorizing effect compared to a reference sample that does not contain components of the claimed composition. Each of the components contributes to the overall effect, and their combination allows to achieve the neutralization of unpleasant odors up to 100%, depending on the concentration of substances according to the invention (Table JV ° 5). The synergistic effect of lipase and b-cyclodextrins is manifested in an increase in the effectiveness of eliminating unpleasant odor by +0.5 points, which is a good change when combining the components in addition to the effects described in examples 1 and 2.

Table JNfs4. Evaluation of the deodorizing effect of the components of the composition

[0176] The base of the composition of the universal surface cleaner will gently clean dirt from surfaces (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in neutralizing persistent unpleasant odors. The study of the deodorizing effect of the composition showed a pronounced synergistic effect in relation to unpleasant odors that are difficult to mask or wash off with conventional surface cleaners based on natural surfactants. The composition makes it possible to replace synthetic unpleasant odor absorbers, synthetic fragrances or aromatic compositions in the composition of the product without loss of effectiveness, which makes it possible to maintain a safe effect on human health, incl. people with allergic respiratory diseases, and provide a high percentage of natural ingredients in the composition of household chemicals for consumers. [0177] Since the composition contains biotechnology-derived lipase and natural b-cyclodextrins, a high % neutralization of unpleasant odors due to sulfur-containing compounds will be provided with a single application. components (mercaptans), organic acids, their esters and other compounds.

The combination of components in the composition allows you to specifically eliminate the cause of unpleasant odors, and not temporarily mask with the help of aromatic substances with a more pronounced intensity of perception.

[0178] Thus, the combination of components allows to achieve a synergistic effect in relation to persistent unpleasant odors due to targeted action and high affinity for organic odor molecules, which allows you to regularly use it as part of household chemicals for various surfaces and control the atmosphere in the room for the psychological comfort of a person .

Example 4

[0179] Mix 9.00 g of glyceryl oleate contained in various fats and vegetable oils, and 90.7 g of purified water until a homogeneous mixture is heated to 45 ° C and stirring at 200 rpm using a magnetic stirrer. Then stop heating and cool to room temperature. In a separate container, a dispersion of 0.0027 g of lipase with an activity of >100 LU/g and 0.27 g of b-cyclodextrins, constituting 3% of the mass of the lipophilic substrate, was prepared in 0.02 g of a mixture of the dispersion medium (glycerol-sorbitol) in a ratio of 1 :1,4. Next, a water-glycerin solution of the components is introduced with active stirring into the medium with the substrate and incubated at 30°C for 4 hours. The initial pH of the solution was 8.20±0.10. [0180] The degree of hydrolysis is determined by measuring pH by isolating a weak oleic acid having pKa(Acid) = 4.8±0.1

[https://echa.europa.eu/registration-dossier/-/registered-dossier/12335/4/22]. In particular, at a ratio of substrate and aqueous phase of 1:3333, a degree of hydrolysis of 76% was achieved in 270 minutes, which indicates a high washing efficiency of the composition.

Example 5

[0181] An instrumental physicochemical study was conducted to confirm the synergism of lipase and b-cyclodextrins when the composition was used together. Spectroscopy methods, in particular UV spectroscopy and fluorescence spectroscopy, are the most suitable for describing biological properties and interactions in organic molecules, in particular enzymes, polysaccharides, etc. Aromatic amino acids in the composition of lipase, such as tryptophan, tyrosine, phenylalanine and histidine , have absorption in the ultraviolet region of radiation (180-400 nm) and emit fluorescence quanta. b-cyclodextrins interact with amino acid residues of lipase subunits, forming clathrate complexes on the surface of the enzyme, which changes the microenvironment chromophore groups and thereby changes the ability to absorb and emit light quanta in different regions of the spectrum.

[0182] For the study, solutions of lipase 2 g/l were prepared with a dispersion of b-cyclodextrins at a concentration of 0 to 4.54 g/l at pH 7.4, taking into account the technological aspect of obtaining the composition described in example 6.

[0183] For UV spectroscopy, absorption spectra were determined at a wavelength of 180-340 nm and the optical density of solutions A was calculated, indicating the absorption of lipase amino acids and an energetically favorable conformation of the enzyme for the enzymatic cleavage of triglycerides in lipid and complex protein-lipid contaminants. According to the results of the study, it was found that at an absorption maximum of 190-200 nm, the spectrum of lipase practically does not change, however, in the region of 270-290 nm, there is an additional peak with a modified spectrum when a dispersion of b-cyclodextrins is added. The optical density of the peak at 270–290 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returns to its original value, which indicates the formation of a stable clathrate complex between lipase and b-cycle o-dextrins. It should be noted that the optimal ratio of lipase-aciclodextrin is from 1:15 to 1:40 without changing the effectiveness of the composition.

[0184] For fluorescence spectroscopy, the emission spectra of fluorescence quanta were determined at a wavelength of 280-450 nm and the relative fluorescence intensity F was calculated, indicating an energetically favorable conformation of the enzyme and the ability of amino acid residues to absorb and emit light quanta. According to the results of the study, it was found that at an absorption maximum of 350-360 nm, the emission spectrum does not change when a dispersion of b-cyclodextrins is added. The fluorescence intensity of the peak at 340–350 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returns to its original value, which indicates the formation of a stable clathrate complex between lipase and b-cyclodextrins. It should be noted that the optimal ratio of lipase-aciclodextrin is from 1:15 to 1:40 without changing the effectiveness of the composition.

Example 6

[0185] The method of obtaining funds according to the invention includes the following steps.

Obtaining a dispersion of lipase and b-cyclodextrins.

[0186] The main process of preparing household chemicals is preceded by the stage of preliminary application of a lipase solution to a dispersion of b-cyclodextrins. The powder of b-cyclodextrins is added to the dispersion medium, the ratio of cyclodextrins to the dispersion medium is 1:1.20-2.50. The process is carried out at room temperature and atmospheric pressure. Cycle o dextrins are added in small portions, with continuous stirring. After adding the last portion of cyclodextrins, the dispersion is stirred for 10-15 minutes, the signal for the transition to the next stage of the process is the absence of large inclusions of cyclodextrins in the dispersion. Then, a lipase solution is added to the resulting dispersion in small portions, the ratio of cyclodextrins (by dry matter) to the lipase solution is 1: 0.5-

1.50. After adding the last portion of lipase, the dispersion is stirred for

10-15 minutes.

Obtaining household chemicals with this composition.

[0187] After settling, the resulting dispersion of lipase and b-cyclodextrins is dissolved in the main reactor to prepare the finished household chemicals, for example, dishwashing detergent. The dispersion is pumped into preheated water, the resulting solution is mixed until completely dissolved. When technically possible, a high-speed disperser is used to intensify dissolution. After the dissolution of the dispersion is completed, the following components specified in the description of the invention can be loaded into the reactor for preparing the finished product, according to the technical recipe.

Claims

CLAIM

1. Composition intended for use in household chemicals, consisting of lipase and b-cyclodextrin, where the mass ratio of lipase and b-cyclodextrin is (0.0025-0.25):(0.1-1), respectively.

2. Composition according to claim 1, characterized in that the amount of lipase in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25): (0.1-1) is 0.0025, 0.005, 0.0075 , 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0 ,25.

3. Composition according to claim 1, characterized in that the amount of b-cyclodextrin in the specified mass ratio of lipase and b-cyclodextrin (0.0025-0.25): (0.1-1) is 0.1, 0.15 , 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0 .8, 0.85, 0.9, 0.95 or 1.

4. Composition according to claim 1, characterized in that said lipase is in a water-glycerol solution.

5. Composition according to claim 4, characterized in that said aqueous glycerol lipase solution is a commercially available product Lipex® Evity® 200 L.

6. Composition according to claim 5, characterized in that said aqueous glycerol lipase solution is a commercially available product Lipex® Evity® 200 L modified with an additional amount of glycerol.

7. The composition according to p. 6, characterized in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerol, and b-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. to the mass of the composition.

8. Composition according to claim 6, characterized in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerol, the mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is (0, 1-1) :(0.5-1.5) respectively.

9. Composition according to claim 8, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.1, 0.15, 0.2, 0, 25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95 or 1.

10. Composition according to claim 8, characterized in that the amount of additional glycerol introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and additional glycerol introduced for modification is 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5.

11. Composition according to claim 1, characterized in that it additionally contains decylglucoside.

12. Composition according to claim 11, characterized in that said decyl glucoside is a commercially available Natural APG HG0814CM product.

13. Composition according to claim 12, characterized in that the mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14).

14. Composition according to claim 13, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0 ,6.

15. Composition according to claim 13, characterized in that the amount of NaturalAPG HG0814CM in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13 ,5 or 14.

16. The composition according to p. 1, characterized in that the composition contains a commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerol, b-cyclodextrin and a commercially available product NaturalAPG HG0814CM, where the content of a commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. to the mass of the composition.

17. Composition according to claim 1, characterized in that the activity of said lipase is at least 40 LU/r or 40 LU/ml at pH 7.0.

18. Composition according to claim 1, characterized in that said household cleaning agent is selected from a dishwashing detergent, a floor cleaner, and/or a toilet bowl, and/or a sink, and/or a bathtub, and/or glasses, a means for pipe cleaners, laundry detergents, including delicate laundry detergents or baby laundry detergents, laundry conditioner, laundry pre-treatment and laundry stain remover, laundry gel and fabric softener.

19. Dishwashing detergent containing 0.0525-1% wt. compositions according to any one of paragraphs. 1-18.

20. Means for washing floors, and/or toilets, and/or sinks, and/or baths, and/or glasses, containing 0.0525-1% wt. compositions according to any one of paragraphs. 1-18.

21. Universal cleaner for washing surfaces containing 0.0525-1% wt. compositions according to any one of paragraphs. 1-18.

22. Tool for cleaning pipes containing 0,0525-2 wt.%. compositions according to any one of paragraphs. 1-18.

23. The tool according to p. 22, characterized in that the specified tool contains 0.0525-1.5% wt. the specified composition, preferably 0,0525-1%wt. specified composition.

24. A laundry detergent containing 0.0525-1% wt. compositions according to any one of paragraphs. 1-18.

25. Means according to claim 24, characterized in that said means is selected from a means for delicate laundry and a means for washing baby clothes.

26. An agent according to claim 24, characterized in that said agent is a powder laundry detergent.

27. The use of a composition according to any one of paragraphs. 1-18 to regulate the kinetics of enzymatic lipid breakdown and neutralize unpleasant odors on various surfaces, maintain long-term cleanliness and a pleasant aroma.

28. Use according to claim 27, characterized in that said surface is selected from metal, polymer, enamel, glass or wood.

29. The method of obtaining a composition according to any one of paragraphs. 1-18, which includes the preliminary introduction of a lipase solution into a dispersion of b-cyclodextrin, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes.