RU2811018C2 - Composition based on lipase and beta-cyclodextrins for regulating the kinetics of enzymatic fat breakdown and washing surfaces - Google Patents

Abstract

FIELD: household chemicals.

SUBSTANCE: composition intended for use in household chemicals contains lipase and β-cyclodextrin, where the mass ratio of lipase and β-cyclodextrin is (0.0025-0.25):(0.1-1), respectively, and the specified lipase is in an aqueous-glycerol solution, which is a commercially available product Lipex® Evity® 200 L, modified with additional glycerin. Also the following is disclosed: another version of the composition for use in household chemicals, household chemicals and the use of the composition for regulating the kinetics of enzymatic breakdown of lipids and neutralizing unpleasant odors, maintaining long-term cleanliness and a pleasant aroma.

EFFECT: group of inventions provides effective regulation of the kinetics of enzymatic lipid breakdown with simultaneous effective neutralization of unpleasant odors on various types of surfaces.

24 cl, 5 tbl, 6 ex

Description

TECHNICAL FIELD

[1] The invention relates to a composition and its use in household chemicals, where the composition consists of lipase and β-cyclodextrin, and the mass ratio of lipase and β-cyclodextrin is (0.0025-0.25):(0.1-1) respectively. The invention provides effective regulation of the kinetics of enzymatic breakdown of lipids with simultaneous effective neutralization of unpleasant odors on various types of surfaces, including especially metal, polymer, enamel, glass and wood, while maintaining long-term cleanliness and a pleasant aroma.

BACKGROUND ART

[2] The development of household chemicals with improved properties for consumers remains one of the promising areas in the category of home care products. Experts estimate that in the UK alone, by the end of 2020, the cleansing products category will grow by 9% CAGR and amount to £485 million compared to 2019 [Dishwashing products: Impact of COVID-19, UK, July 2020, Mintel], and in China, the market size is increasing by 4% CAGR annually and will reach RMB17.195 million by 2024 [Dishwashing Products - China - February 2020, Mintel].

[3] Every day a person uses a sufficient amount (>3) of household chemicals to clean various surfaces of the house: wooden, ceramic, enamel, metal, polymer, porcelain, etc. Consumers more often pay attention to the functional characteristics of the product of choice, especially the removal efficiency household contaminants (types of contaminants, difficulty of removing them, speed of purification), additional effects, for example, elimination of unpleasant odors in the house, safe effects on cleaned surfaces and the skin of the hands, as well as safety for the environment and the possibility of using products in houses with independent sewerage and septic tanks. Based on a Lightspeed/Mintel survey in March 2019, more than 38% of UK consumers prefer to choose multi-functional household cleaning products that reduce the amount of time it takes to complete a thorough cleaning. According to the analytical review of Dishwashing products: Impact of COVID-19, UK, July 2020 Mintel, by 2020-2025. There will be a growing trend for products with sustainable development (sustainable products), with a high content of natural ingredients, a long list of ingredients not contained in products (free from list). It is worth noting that the consumption of household chemicals in the EMEA region does not depend on the income level of the population, since such products are essential goods. However, according to the results of a Lightspeed/Mintel survey in March 2020, 10-16% of consumers over the age of 18 began to use household chemicals less frequently due to low efficiency (10% of respondents), high content of synthetic chemicals (11% of respondents), high water consumption to remove contaminants (36% of respondents). Additionally, an assessment was made of the attractiveness and possibility of purchasing household chemicals in the EMEA region. Thus, the main stimulating factors are a reduction in physical effort to remove dirt by hand (more than 33% of answers), a decrease in water consumption to wash off dirt (more than 28% of answers) and the high proven effectiveness of the product (more than 27% of answers). Thus, the need has been identified for the development of innovative household chemicals that can effectively remove household contaminants, reduce the effort spent on cleaning surfaces and are safe for nature and humans.

[0004] However, despite the demand for effective products, consumer attention remains to the dermatological comfort of the skin of the hands after using household cleaning products and detergents. Every day a person comes into contact with a large number of surfaces in public places and at home, and also uses household chemicals. In order to protect yourself from the development of dermatological diseases, it is necessary to take care of the choice of household chemicals, since it is the dermatological comfort of the hands that is the key to health and, accordingly, a high quality of life for people. Due to frequent hand contact with synthetic ingredients in skin products, formulating products with a high content of natural ingredients remains the most reliable way to maintain healthy skin and, therefore, a healthy appearance. According to experts, skin health is one of the fundamental foundations of overall human health. Thus, 2% sodium lauryl sulfate, known as SLS, in household chemicals can cause a loss of transepidermal moisture by 68.9 g/m2/h relative to the normal level 12 hours after contact with the product [Loffler, N., & Happle, R (2003). Profile of irritant patch testing with detergents: sodium lauryl sulfate, sodium laureth sulfate and alkyl polyglucoside. Contact Dermatitis, 48(1), 26-32]. Since the content of sodium lauryl sulfate in household chemicals can reach up to 29% due to its high cleaning power, this can negatively affect the condition of the skin of the hands. C10-16 alkyl sulfates cause denaturation of keratin due to the destruction of sulfide bonds and the formation of sulfhydryl groups -SH on human cells and leaching of the epidermal barrier [Prottey C, Ferguson T. Factors which determine the skin irritation potential of soaps and detergents. J Soc Cosmet Chem. 1975;26: 29-46.], while alkyl sulfates with a chain length of C12-C14, which include SLS, have the maximum activity. It was revealed that aggressive anionic surfactants sodium dodecyl sulfate (SDS), sodium myristyl sulfate (SMS), sodium tridecyl sulfate (STS) cause leaching of water-soluble epidermal proteins by 166.1%, 163.9% and 198.5%, respectively [Loffler, N., & Happle, R. (2003). Profile of irritant patch testing with detergents: sodium lauryl sulfate, sodium laureth sulfate and alkyl polyglucoside. Contact Dermatitis, 48(1), 26-32]. Thus, the development of formulas for detergents and cleaning products with household chemicals with a reduced content of synthetic and semi-synthetic anionic surfactants is a priority for caring for the epidermis of the skin of the hands.

[0005] The search for effective components and their combinations as means for removing household contaminants of various natures, in particular lipid contaminants, is one of the priorities of companies involved in the production of detergents and cleaning products. The main components of household chemicals are surfactants, fillers, components that ensure stability of formulations, and functional additives that perform specific functions. In particular, special functional additives include lipase and beta-cyclodextrins.

[0006] Lipase or triacylglycerol acyl-gyrolase (enzyme classification code E.C.3.1.1.3, CAS 9001-62-1, EINECS 232-619-9) is a water-soluble enzyme from the class of hydrolases that catalyzes the hydrolysis of ester bonds in triglycerides of fatty acids, which are water-insoluble esters of glycerol and higher carboxylic acids of various structures [European Commission Cosmetic Ingredients & Substances Database: http://ec.europa.eu/growth/tools-databases/cosing/]. Lipase is registered as a food additive E1104 and can be used to digest, dissolve and fractionate fats.

[0007] Lipase is a catalyst for the fat breakdown reaction and is not a product/starting material in the hydrolysis reaction, which allows for improved enzymatic reaction kinetics. It is known that one enzyme molecule can catalyze up to 10,000 reactions per second, depending on the domain organization, functional activity and raw materials. A small amount of enzyme is sufficient for effective action, since the activity of commercially available lipases is above 40 U/g or U/ml. Thus, the effective concentration of lipase is 0.4-0.8% (w/w) as part of complex additives for use in household chemicals [https://www.enzymeirmovation.com/lipase-detergent-everything-you-need- khow/]

[0008] It is believed that lipases are one of the most effective enzymes for removing grease stains and lipid contaminants based on vegetable oils (sunflower, olive, rapeseed, corn, flaxseed, etc.) with different fatty acid compositions, animal fats (butter, lard, beef fat, lamb fat, etc.), having a solid state of aggregation under normal conditions, lubricants based on ester components and fatty phases of perfumery and cosmetic products containing oils, waxes, emulsifiers with ester bonds, such as lecithin, components of fragrances and essential oils. Since these stains are insoluble in the aqueous phase and are removed only with the help of micellar solutions of surfactants with limited solubility, lipase promotes the hydrolysis of ester bonds and the destruction of substrates, due to which surfactants of any nature better remove stains from the surface and penetrate deep into the tissue and reach saturation of micelles more slowly while maintaining cleaning efficiency.

[0009] Lipases are highly active against lipid contaminants on various surfaces, including metal, polymer, wood, ceramic, and do not disrupt the structure of these surfaces, which indicates a gentle effect in detergents. The most desirable pH optimum is from 5.0 to 11.0, and activity can be maintained at various temperatures from 0°C to 60°C, in particular the temperature optimum is from 20°C to 40°C. Lipases can be stable in the presence of proteases, chelating agents, peroxide compounds (hydrogen peroxide, sodium percarbonate, etc.) and surfactants, in particular anionic ones. Lipase stabilizers can be added to the systems, in particular glycerin, propylene glycols, sorbitol, sugars, carboxylic acids, alkylamines, inorganic salts, nonionic and ionic surfactants, as well as a reduced water content in the formulation, which makes the detergent concentrated and with low water consumption at production of detergents. The use of lipase makes it possible to produce energy-efficient products that save energy and water, thereby responsibly using resources.

[0010] The concentration of lipase in household chemicals depends on the activity and can range from 0.0025 to 1% (by weight, in pure form). It was found that the addition of lipase with an activity of 100 KLU3/g to surfactants in the detergent composition showed high efficiency in removing greasy stains during standard washing at 40°C. The effective concentration of lipase (as part of complex additives) with this activity is 0.2-0.6% [N. Uhlig, E.M. Linsmaier-Bednar. Industrial enzymes and their applications. Engineering, April 1998, 472 pages. ISBN: 978-0-471-19660-0. DOI:10.5860/choice.36-0333]. In detergents, lipase with an activity of 50 U/mL in an amount of 10 mL together with a 0.5% system of anionic and nonionic surfactants was stable in the formulation and did not affect the thermal stability of the detergent. Also, lipase was not destroyed in the presence of a 2% hydrogen peroxide solution, i.e. retained its activity by 92% after 2 hours of adding a bleach solution to the solution, however, the activity dropped significantly in the presence of sodium hypochlorite and sodium perborate when the concentration was increased from 1 to 2%.

[0011] Lipase has been noted to be a necessary component in detergents for the effective removal of lipid stains and grease stains. It is the enzymatic activity per unit volume that determines the working concentration of the component in the formulations of detergents for various purposes. With a temporary activity of 50 U/mL and in an amount of 10 mL, the total enzyme activity will be 500 U. With an average enzyme activity of 100-1000 U/g, the effective concentration of lipase (as part of a complex additive) will be 0.05-5.00 % in detergent formulation [Mamta Chauhan, Rajinder Singh Chauhan, and Vijay Kumar Garlapati. Evaluation of a New Lipase from Staphylococcus sp.for Detergent Additive Capability. BioMed Research International Volume 2013 |Article ID 374967 | 6 pages | https://doi.org/l0.1155/2013/374967. https://www.hindawi.com/journals/bmri/2013/374967/].

[0012] Thus, lipase is a necessary component in detergents to effectively remove lipid stains and grease stains by targeting fatty acid triglyceride molecules and enhancing the effectiveness of surfactant-based detergent systems, particularly non-ionic surfactants. With an average activity of the component >100 U/g, the effective concentration of lipase in its pure form is 0.0025-0.1% in cleaning agents and detergents for cleaning various surfaces.

[0013] At the same time, lipase has a specific odor. Lipase breaks down fats into glycerol and fatty acids. If, for example, these fatty acids are not completely removed from textiles during the washing process, consumers notice a rancid odor that becomes more intense depending on the amount of lipase. (Averyanova V.A. Evolution of detergent properties: the pursuit of a sustainable ideal / Raw materials and packaging. No. 02 (151), 2014). Thus, lipase can lead to irritation of the mucous membranes of the upper respiratory tract and lungs and lead to an allergic reaction. The inventors have successfully overcome this limitation by creating an effective combination.

[0014] Another functional biodegradable component is cyclodextrins. The area of control over aromas and atmosphere in the home remains relevant and is actively developing. Smells play an important role in human life, as they allow us to recognize various compounds and navigate in space, as well as protect ourselves from toxic and allergenic substances. It is not always possible to neutralize the unpleasant odor in the room, which causes a feeling of discomfort and does not create a feeling of safety for a person. Not all household chemicals have the ability to neutralize unpleasant odors after a thorough cleaning of the house, so there is a need to manage odors to create a comfortable environment.

[0015] Cyclodextrins are substances of natural origin, cyclic oligomers of glucose obtained enzymatically from starch [Crini G. A history of cyclodextrins // Chemical reviews. - 2014. - T. 114. - No. 21. - P. 10940-10975]. Cyclodextrins contain at least six D-(+) glucopyranose residues linked by α-(1,4)-glucosidic bonds. Glucopyranose residues form an energetically favorable “chair” conformation, which is why cyclodextrin molecules have the shape of cones. The outer surface of the latter is hydrophilic and allows them to be water-soluble, and the central cavity has hydrophobic properties due to the specific arrangement of hydroxyl groups and allows the incorporation of hydrophobic or amphiphilic substances. Natural cyclodextrins are of three types: α, β and γ, which correspond to 6, 7 and 8 glucopyranose residues [B. Bonthagarala, CH BABU RAO, N. Sreekanth. The cyclodextrins: A review // IJPRBS - 2013. - T. 2. - P. 291-304]. Each of the three types of cyclodextrins has its own physicochemical properties, in particular molecular weight, central diameter of the cavity, approximate volume of the cavity and solubility in water, the number of water molecules contained in the cavity of cyclodextrins and pKa of the substance in an aqueous environment. Thus, β-cyclodextrin molecules have the largest internal cavity diameter, which allows them to form inclusion complexes (host-guest complexes) with a wide range of solid, liquid and gaseous compounds through molecular complexation. In these complexes, the guest molecule is retained in the cavity of the host cyclodextrin molecule due to hydrophobic and van der Waals interactions. The hydrophobic cavity of cyclodextrin molecules provides a microenvironment in which non-polar fragments of suitable size can be introduced to form an inclusion complex [Loftsson T., Brewster M. E. Pharmaceutical applications of cyclodextrins. 1. Drug solubilization and stabilization // Journal of pharmaceutical sciences. -1996. - T. 85. - No. 10. - pp. 1017-1025]. In this case, there is no formation or destruction of covalent bonds, which lead to the destruction of molecules. The main driving force for complex formation is the release of water molecules from the cavity of cyclodextrins, rich in enthalpy ΔH. Water molecules are displaced by a more hydrophobic “guest” under the influence of non-covalent forces of intermolecular interaction, due to which the ΔG free energy of the surface of the cyclodextrin ring decreases, and the cyclodextrin molecule is stabilized in a low-energy state [Szejtli J. Introduction and general overview of cyclodextrin chemistry // Chemical reviews. - 1998. - T. 98. - No. 5. - C. 1743-1754]. The binding of guest molecules is characterized by dynamic equilibrium. The strength of binding depends on how strong the non-covalent host-guest interactions are. Complexes can form either in solution or in a crystalline state, with water usually being the preferred solvent [Hǎdǎrugǎ N. G. et al. A review on thermal analyzes of cyclodextrins and cyclodextrin complexes // Environmental Chemistry Letters. - 2019. - T. 17. - No. l. - pp. 349-373].

[0016] Complexation allows you to change some properties of the “guest” molecules: increase the solubility of hydrophobic molecules, increase the stability of labile molecules, reduce the volatility of aromatic compounds [V. Bonthagarala, CH BABU RAO, N. Sreekanth. The cyclodextrins: A review // IJPRBS - 2013. - T. 2. - P. 291-304]. The list of potential "guests" for molecular encapsulation in cyclodextrins is very diverse and includes compounds such as straight and branched chain aliphatics, aldehydes, ketones, alcohols, organic acids, fatty acids, aromatic compounds, gases and polar compounds such as halogens, hydroxy acids and amines [Del Valle E. M. M. Cyclodextrins and their uses: a review // Process biochemistry. - 2004. - T. 39. - No. 9. - pp. 1033-1046].

[0017] For many encapsulated substances, the cavity size of α-cyclodextrins is too small, and the use of γ-cyclodextrins is often limited by their high production cost. Therefore, β-cyclodextrins are used more widely in various industrial fields compared to other natural cyclodextrins: they have a satisfactory cavity size, and their production and purification are more economical [Lovatti Alves Q. et al. Drugs-β-Cyclodextrin inclusion complex: Would be a new strategy to improve Antihypertensive Therapy // Clin. Res. Trials. - 2019. - T. 5. - P. 1-3].

[0018] β-Cyclodextrins have various effects, in particular, they regulate the simultaneous absorption and prolonged release of odor molecules, including unpleasant ones, the kinetics of coloring and release of dyes during washing, the stabilization of aromatic compounds (fragrances, fragrances, essential oils) in formulas and provide thermal stability of household chemicals and perfumes and cosmetics.

[0019] Residual surfactants on the surface of the fibers affect the absorbency of the textile surface and the dyeability of textiles, and also reduce the quality of the hydrophobic finish. Cyclodextrins are capable of forming complexes with surfactants in aqueous solution, which allows the removal of most of the absorbed surfactants. Addition of cyclodextrin (3 g/l) to rinse water eliminates residual traces of detergent in the solution and thus reduces residual detergent in fabrics (from 209 to 134 ppm) and water consumption [Ammayappan L., Moses J. J. An overview on the application of cyclodextrins in textile product enhancement // J Text Assoc. - 2009. - T. 70. -№l. - P. 9-18].

[0020] Promising directions for the use of cyclodextrins in the process of dyeing fabrics were described in detail [Bezerra F.M. et al. The Role of β-Cyclodextrin in the Textile Industry //Molecules. - 2020. - T. 25. - No. 16. - P. 3624]. Cyclodextrins, when used as dyeing auxiliaries, affect both dye properties and dyeing kinetics, allowing for increased extraction of excess dye and thereby improved wastewater quality and environmental burden.

[0021] Molecules or functional groups that cause an unpleasant taste or odor can be hidden from sensory receptors by enclosing them in the cavity of cyclodextrins. Such complexes significantly reduce the concentration of free molecules that cause unpleasant taste or odor [Tiwari G., Tiwari R., Rai A.K. Cyclodextrins in delivery systems: Applications // Journal of Pharmacy and Bioallied Sciences. - 2010. - T. 2. - No. 2. - P. 72], in particular dihydroxyacetone, free fatty acids and esters, amine derivatives, glutathione, mercapto compounds, capronaldehyde as a marker of the oxidation of vegetable oils and fats. Cyclodextrins, by complexing with aromatic compounds, can be used to mask strong odors or reduce the intensity of aromas.

[0022] A major ventilation problem in home or commercial kitchens is the removal and separation of volatile compounds, which are perceived as strong odors, especially released when frying food with vegetable oils and fats. Cone-shaped molecules of β-cyclodextrins make it possible to adsorb strong and pungent odors of capronaldehyde, which is a marker of oxidation of oils and fats during cooking [Ghorani B. et al. Assembly of odour adsorbent nanofilters by incorporating cyclodextrin molecules into electrospun cellulose acetate webs // Reactive and Functional Polymers. - 2019. - T. 134. - P. 121-132], in the process of washing various kitchen surfaces and dishes after frying food. Since fats and oils contain esters of fatty acids and glycerol, a complex based on a specific lipase enzyme and β-cyclodextrins improves the washing process due to the targeted breakdown of fat molecules and the absorption of released fatty acids. This synergistic effect improves the cleaning ability of household chemicals for various surfaces due to the regulation of the kinetics of fat breakdown and the absorption of unpleasant odors caused by fatty acids and their oxidation products, unlike other detergents.

[0023] In documents EP 0427806 B1 [NOVO NORDISK A/S] publ. 06/20/1994 and WO 1990010687 A1 publ. 09/20/1990 describes the process of hydrolysis of fats in the presence of lipase in water in a ratio of 1:3 to 3:1 (by weight), characterized by the presence of α-, β- or gamma-cyclodextrins, which can optionally be replaced. Cyclodextrins improve the degree of hydrolysis of fats or oils, i.e. any material with a high triglyceride content, using lipase in an aqueous environment. The combination can be used with a constant dosage of lipase and unchanged reaction time, using increased pressure to homogenize the solution.

[0024] The composition described above from patent application WO 1990010687 A1 has a number of disadvantages, in particular the inability to use at high temperatures above 60°C due to denaturation of the quaternary structure of the enzyme, which leads to its inactivation and reduced efficiency of the lipid hydrolysis process, as well as the use elevated pressure more than 0.101325 MPa, which requires special technological equipment and confirmation of lipase activity at this pressure. Lipase activity at low temperatures and a wide temperature range (temperature lability) would make it possible to expand the area of application of this combination. Higher fat:water and water:lipase ratios would allow the process to scale up and increase the yield of hydrolysis products, since less fat and lipase would be required for the process. The use of lipase of animal origin is also described, which indicates a lack of awareness of the environment and will not allow obtaining voluntary Vegan/Vegetarian certification for household chemical products containing this component. A reduction in the amount of lipase in the technological process can be achieved by adding β-cyclodextrins, which act as colipase and activator of the kinetics of the breakdown of triglycerides of fatty acids.

[0025] In patent application WO 01/18163 A1 [THE PROCTER & GAMBLE COMPANY] publ. 08/21/2000 disclosed the use of a composition for the preparation of a laundry detergent containing a granular cycle of dextrin. The composition ensures the elimination of unpleasant odors from things associated with the presence of particles of sweat, sebum and sebum, dead skin cells, during automatic washing in the presence of cyclodextrin in the detergent. The active form of stable, quickly soluble, free-flowing cyclodextrin in granular form has a deodorizing effect and is capable of smoothing laundry after washing, mainly cotton, as well as for washing other surfaces, such as upholstery, curtains, carpets, rugs, etc. The component can be used together with enzymes (protease, cellulase, lipase, amylase, and/or a mixture of these enzymes) and excipients.

[0026] The above-described composition from patent application WO 01/18163 A1 contains only granular cyclodextrins as components for odor absorption, but is not capable of specifically breaking down fatty acid triglycerides and increasing the effectiveness of detergents in this direction, since lipase is not the main active component of the composition . Due to its medium particle size, granular cyclodextrin can only be used as part of fillers, powder mixtures or washing powders for washing clothes, which limits the scope of application of the component, since it is difficult to introduce it into liquid detergents to achieve this effect due to its low solubility. The introduction of a specific lipase enzyme would improve the efficiency of removing fatty stains and eliminating odors due to the presence of specific esters responsible for unpleasant odors, and non-granulated cyclodextrins are more easily soluble in water when stirred and are stable in liquid detergents, which expands the scope of application of the components. Additionally, non-granular cyclodextrins would absorb the reaction products of the hydrolysis of lipid substrates, allow the kinetics of fat breakdown to be regulated, absorb unpleasant odor molecules due to the hydrophobic cavity, and reduce the amount of water and electricity consumed per 1 wash of laundry.

[0027] In patent application EP 1075509 A1 (WO9957254A1) [THE PROCTER & GAMBLE COMPANY] publ. 04/30/1999 disclosed the use of a composition for the preparation of laundry detergents containing a modified transferase. The modified enzyme contains a catalytically active transferase domain linked to the amino acid sequence of the cellulose binding domain to effectively remove stains and improve the washing process. The component can be used in conjunction with a system of surfactants, enzymes (protease, cellulase, lipase, amylase and/or a mixture of these enzymes), bleaching agents, fillers, releasing agents, optical brighteners, fabric softeners, dispersing agents, dye leaching inhibitors from fabrics , abrasives, bactericidal substances, fragrances. The composition based on a modified transferase is aimed at removing body sebum and contaminants based on plant fibers, sugars, amylose, which are difficult to remove during the washing process, especially at low temperatures. Thus, a need is described for a composition for removing soiling, smoothing and softening laundry, controlling static stress, preserving color and increasing the durability of fabric.

[0028] The composition described above from patent application EP1075509A1 (WO9957254A1) is intended for the specific cleavage of polysaccharide substrates, in particular glycosidic dimers, oligomers and/or polymers of starch, xyloglycans, cyclodextrins, sucrose and maltose. Cyclodextrins do not increase the effectiveness of the composition, since they are substrates of the enzyme that breaks them down. The enzyme lipase is described as a possible additional enzyme in this composition along with protease, cellulase, and amylase. In the examples given, the lipase content varies from 0.002 to 0.01%, which does not allow the complete breakdown of lipid contaminants in short laundry programs (up to 30 minutes). The composition does not have the ability to specifically absorb unpleasant odors that are fixed by sebum and lipid deposits, but only partially removes them during the process of washing and drying clothes. The use of a lipase enzyme with high activity and an increase in its content would improve the efficiency of removing fatty contaminants and eliminating odors due to the presence of specific esters responsible for unpleasant odors, and non-granulated cyclodextrins would absorb the reaction products of the hydrolysis of lipid substrates, would allow regulating the kinetics of fat breakdown, would absorb molecules of unpleasant odor due to the hydrophobic cavity and would reduce the amount of water and electricity consumed per 1 wash of clothes.

[0029] In contrast to patent applications WO1990010687A1, WO01/18163A1 and EP1075509A1 (W09957254A1), the present inventors investigated the effectiveness of a biodegradable composition containing biotechnology-derived lipase and β-cyclodextrins, and found a synergistic effect to regulate enzyme kinetics and rapid degradation lipid stains and deposits from various surfaces (wood, metal, enameled, polymer, ceramic, earthenware, etc.), additional emulsification of fats and fatty acids, absorption of unpleasant odors from various surfaces, ensuring the stability of liquid detergents with aromatic components during storage. The combination safely acts on the skin of the hands and maintains dermatological comfort of the skin, which allows reducing the content of anionic surfactants that cause increased sensitivity of the skin of the hands and does not cause irritation. Thanks to the synergistic effect of lipase and P-cyclodextrins, it becomes possible to reduce the content of anionic surfactants or combinations with them by 30% or more. The addition of β-cyclodextrins makes it possible to reduce the reaction temperature and reduce the peroxidation of triglycerides of unsaturated fatty acids, which are found in soybean, corn, flaxseed, olive and other edible oils. During oxidation, acid oxides and peroxides are formed, which have a specific rancid odor, give a yellowish color and cause clouding of dishes made of polymer materials.

[0030] The technical result of the innovative complex is the effective removal of fatty contaminants by regulating the kinetics of the breakdown of lipid contaminants, eliminating unpleasant odors and maintaining the thermal stability of samples. The complex is active in a wide pH range, in particular 5.0-10.0 units, and a wide temperature range, in particular from +5 to +60°C, which expands the scope of application in detergents and cleaning products.

[0031] It has been discovered that the combination of lipase and β-cyclodextrins can increase the removal efficiency of complex protein-lipid stains on various surfaces, in particular metal, polymer, glass and wood surfaces, and also increase the cleaning efficiency of the products by more than 35% on various surfaces while maintaining the surfactant content based on components of natural origin. In this case, lipase and β-cyclodextrins mean raw materials containing the corresponding active components, as well as technical impurities that could be formed during the production of the target raw materials.

[0032] Combining components allows for synergistic effects and maintains efficiency at a lower percentage of input of individual components. Lipase, obtained by modern methods of biotechnology without the use of GMOs, is an active enzyme that breaks down insoluble lipid substrates with ester bonds of triglycerides at the water-oil or oil-water interface. β-Cyclodextrins act as coactivators (colipases) by binding to the terminal domain of lipase and forming a clathrate shell of water molecules, promoting a change in the conformation of the active center of lipase and reducing the activation energy of the process (ΔE _a ), which facilitates the enzymatic reaction. The lipase enzyme lowers the activation energy by increasing the number of activated fatty acid triglyceride and glycerol molecules, which become reactive at a lower energy level, which lowers the energy barrier to further enzymatic reaction. Additionally, cyclodextrins, due to the hydrophilic surface caused by free hydroxyl groups -OH, specifically orient lipase substrates at the water-oil or oil-water interface, increasing the availability of the ester bond for enzymatic hydrolysis. Since the enzymatic reaction is an equilibrium reaction, in order to shift the equilibrium of the reaction towards the formation of hydrolysis products, it is necessary to bind and remove free fatty acids from the system. In particular, β-cyclodextrins bind reaction products due to their emulsifying properties, shifting the reaction equilibrium towards the breakdown of lipid substrates in the system.

[0033] An additional property is the neutralization of odorous aromatic substances represented by various classes of organic substances: aldehydes and ketones, terpenes, amines, indole, sulfur-containing components (mercaptans), organic acids and their esters, phenols and cresols. Since a person is more sensitive to unpleasant odors, the neutralization of odorous substances becomes the main tool for managing the emotional state. In such cases, cleaning with conventional household chemicals does not effectively eliminate odors, so a special combination has become necessary to control and eliminate odors in addition to regulating the kinetics of hydrolysis of lipid contaminants.

[0034] An innovative complex, including lipase and P-cyclodextrins, is aimed at effective cleansing and removal of fatty contaminants by regulating the kinetics of the reaction of the breakdown of lipid substrates and increasing the cleaning efficiency of household chemicals, as well as neutralizing unpleasant odors. The complex based on natural and biodegradable components is effective in the pH range of 5.0-11.0 in the presence of various synthetic and natural components, which allows it to be used as part of a wide range of household chemicals for cleaning various surfaces, such as laundry detergents, laundry detergents dishes, floors, glass, universal cleaning products. The components have a targeted effect on lipid and complex contaminants on various surfaces, and also bind the released metabolic products with an unpleasant aroma for humans. After using the composition, the cleanliness of surfaces and a pleasant aroma remain longer. Thus, the combined use of components in the claimed composition in one product leads to increased kinetics of the enzymatic reaction of the breakdown of complex household contaminants with lipids due to stabilization, orientation of substrates and increased lipase activity due to β-cyclodextrins, which allows for rapid cleansing even when using cold water. A distinctive feature is that the components in the declared concentrations act only against complex contaminants and do not affect the consumer appearance of most surfaces, in particular metal, wood, polymer and enameled ones. The composition does not contain aggressive inorganic substances and organic solvents, occlusive film formers and alcohols, so it is possible to use detergents and cleaning products with this composition on a regular basis without harm to the skin of human hands. The combined use of these components exhibits a synergistic effect, providing complete care for various surfaces as part of one household chemical product for daily use.

[0035] Thus, the invention generally relates to a composition and its use to achieve such technical results as effective regulation of the kinetics of enzymatic lipid breakdown while simultaneously effectively neutralizing unpleasant odors on various types of surfaces, including especially metal, polymer, enamel, glass and wooden, maintaining long-lasting cleanliness and a pleasant aroma, which are not achieved or insufficiently achieved by modern commercially available products in the art.

SUMMARY OF THE INVENTION

[0036] In one aspect, the invention relates to a composition for use in a household cleaning product consisting of lipase and β-cyclodextrin, wherein the weight ratio of lipase to β-cyclodextrin is (0.0025-0.25):(0.1 -1) respectively.

[0037] The composition may differ in that the amount of lipase in the specified weight ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0. 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25.

[0038] The composition may differ in that the amount of β-cyclodextrin in the specified weight ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0. 2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95 or 1.

[0039] The composition may differ in that said lipase is present in an aqueous glycerol solution.

[0040] The composition may be characterized in that said aqueous glycerol lipase solution is the commercially available product Lipex® Evity® 200 L. Lipex® Evity® 200 L is available, inter alia, from Novozymes and can be identified, for example, by <https http://biosolutions.novozymes.com/en/laundry/products/lipexr-evityr-200-l>.

[0041] The composition may differ in that said aqueous-glycerol lipase solution is the commercially available Lipex® Evity® 200 L modified with additional glycerin.

[0042] The composition may differ in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerin, and β-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% by weight. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. for the mass of the composition.

[0043] The composition may differ in that in a composition containing Lipex® Evity® 200 L modified with an additional amount of glycerin, the weight ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is (0.1-1):(0 .5-1.5) respectively.

[0044] The composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 ,3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9 , 0.95 or 1.

[0045] The composition may differ in that the amount of additional glycerin introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.5, 0.55, 0.6, 0.65, 0. 7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5.

[0046] The composition may differ in that it additionally contains a decyl glucoside.

[0047] The composition may differ in that said decyl glucoside is the commercially available product NaturalAPG HG0814CM. NaturalAPG HG0814CM is available, inter alia, from Hugo and can be identified, for example, by <https://www.hugochem.net/alkyl-polyglucoside/apg-0814/decyl-glucoside-apg-2000up.html>.

[0048] The composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14).

[0049] The composition may differ in that the amount of Lipex®Evity® 200 L in the specified weight ratio of Lipex®Evity®200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0.6 .

[0050] The composition may be characterized in that the amount of NaturalAPG HG0814CM in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5 or 14.

[0051] The composition may be characterized in that the composition contains commercially available Lipex® Evity® 200 L modified with additional glycerol, β-cyclodextrin and commercially available NaturalAPG HG0814CM, wherein the content of commercially available Lipex® Evity® 200 L is 0. 4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. for the mass of the composition.

[0052] The composition may be characterized in that the activity of said lipase is at least 40 LU/g or 40 LU/ml at pH 7.0.

[0053] The composition may differ in that said household chemical is selected from dishwashing detergent, floor and/or toilet cleaner, and/or sink, and/or bathtub, and/or glass cleaner, pipe cleaner, Laundry detergents, including delicates or baby laundry detergents, fabric softener, pre-wash and pre-wash stain remover, laundry gel and fabric softener.

[0054] In another aspect, the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention.

[0055] In another aspect, the invention relates to a floor, and/or toilet, and/or sink, and/or bath, and/or glass cleaner containing 0.0525-1% wt. compositions according to the invention.

[0056] In another aspect, the invention relates to an all-purpose surface cleaner containing 0.0525-1% by weight of the composition of the invention.

[0057] In another aspect, the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention.

[0058] The pipe cleaner may differ in that said product contains 0.0525-1.5% wt. the specified composition, preferably 0.0525-1% wt. the specified composition.

[0059] In another aspect, the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention.

[0060] The laundry detergent may be characterized in that the laundry detergent is selected from a delicate laundry detergent and a baby laundry detergent.

[0061] The laundry detergent may be characterized in that the laundry detergent is a powder laundry detergent.

[0062] In another aspect, the invention relates to the use of the composition of the invention to regulate the kinetics of enzymatic lipid breakdown and neutralize unpleasant odors on various surfaces, maintaining long-term cleanliness and a pleasant aroma.

[0063] The application may differ in that said surface is selected from metal, polymer, enamel, glass or wood.

[0064] In another aspect, the invention relates to a method for preparing a composition according to the invention, including preliminary addition of a lipase solution to a β-cyclodextrin dispersion, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes.

[0065] The invention will be further described in detail in particular cases of its implementation and illustrated with examples of implementation.

DETAILED DESCRIPTION OF THE INVENTION

[0066] In the agent of the invention, suitable excipients may be selected from the following categories of components.

[0067] Anionic surfactants:

[0068] Salts of higher carboxylic acids with the general formula: R1-CO2X1, where R1 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and XI is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium, basic amino acid;

[0069] Alkyl polyethylene glycol sulfate with the general formula: R2-O(-CH2-CH2-O) nlSO3X2, where nl can take values from 1 to 10, and denotes the number of polyethylene glycol groups, R2 is an alkyl and/or alkenyl group with a long hydrocarbon chains from 6 to 22 carbon atoms, and X2 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0070] Alkyl sulfate with the general formula R3-OSO3X3, where R3 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms, and X3 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium ;

[0071] A salt of a higher fatty acid amide and methylglycine with the general formula R4-C(O)-N(-CH3)-CH2-CO2X4, where R4 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms, and X4 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0072] Alkyl polyethylene glycol carboxylate with the general formula: R5-O(-CH2-CH2-O-)n2CH2-CO2X5, where n2 can take values from 1 to 15, and denotes the number of polyethylene glycol groups, R5 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, and X5 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0073] A disubstituted salt of 2-sulfocarboxylic acid with the general formula: R6-CH(-SO3X6)-CO2X6, where R6 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 4 to 20 carbon atoms, and X6 is an alkali cation and /or alkaline earth metal, ammonium, alkylammonium, alkanolammonia, glucoammonium;

[0074] A mono or disubstituted salt of a higher carboxylic acid amide and glutamic acid with the general formula: R7-C(O)-NH-CH(-CH2-CH2-CO2X7)-CO2X7, where R7 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X7 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium or hydrogen;

[0075] A salt of a higher fatty acid amide and glycine with the general formula: R8-C(O)-NH-CH2-CO2X8, where R8 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms, and X8 is alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonia, glucoammonium cation;

[0076] Salt of a higher fatty acid amide and alanine with the general formula: R9-C(O)-NH-CH(-CH3)-CO2X9, where R9 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , and X9 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0077] A salt of a higher fatty acid amide and 2-aminomethylethanesulfonic acid with the general formula: R10-C(O)-N(-CH3)-CH2-CH2-SO3X10, where R10 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X10 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0078] Alkyl polyglucoside hydroxypropyl sulfonate with the general formula: R11-O-[G]pl-O-CH2-CH(-OH)-CH2-SO3Xll, where R11 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 atoms carbon, G is a saccharide fragment containing 5 or 6 carbon atoms, p1 can take values from 1 to 4, and X11 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0079] Alkyl polyglucoside carboxylate with the general formula: R12-O-[G]p2-O-CH2-CO2X12, where R12 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p2 - can take values from 1 to 4, and X12 - cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0080] Salt of a higher fatty acid amide and threonine with the general formula: R13-C(O)-NH-CH(-CH(-OH)-CH3)-CO2X13, where R13 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X13 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0081] Salt of a higher fatty acid amide and an amino acid obtained by hydrolysis of proteins from plant materials, with the general formula: R14-C(O)-AAX14, where R14 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , AA is an amino acid or peptide obtained by hydrolysis of vegetable protein (possible sources of protein: apple, soybean, wheat, cotton, etc.), and X14 is an alkali and/or alkaline earth metal cation, ammonium, alkylammonium, alkanolammonium, glucoammonium.

[0082] Amphoteric surfactants:

[0083] A disubstituted acylamphodiacetate salt with the general formula: R15-C(O)-NH-CH2-CH2-N(-CH2-CO2X15)-CH2-CH2-O-CH2-CO2X15, where R15 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X15 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0084] An acylamphoacetate salt with the general formula: R16-C(O)-NH-CH2-CH2-N(-CH2-CO2X16)-CH2-CH2-OH, where R16 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and X16 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0085] Alkylamphoacetate salt with the general formula: R17-C(=N-CH2-CH2-N((-CH2-CH2-OH)-CH2-CO2X17)-), where R17 is an alkyl and/or alkenyl group with a long hydrocarbon chain chains from 5 to 21 carbon atoms, and X17 is a cation of an alkali and/or alkaline earth metal, ammonium, alkylammonium, alkanolammonium, glucoammonium;

[0086] Acylamidoalkyl betaine with the general formula: R18-C(0)-NH-R19-N(-CH3)2)-CH2-CO2, where R18 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms , R19 is an alkyl group with a long hydrocarbon chain of 1 to 4 carbon atoms;

[0087] Acylamidoalkylhydroxysultaine with the general formula: R20-C(O)-NH-R21-N(-CH3)2-CH2-CH(-OH)-CH2-S03, where R20 is an alkyl and/or alkenyl group with a long hydrocarbon chain chains from 5 to 21 carbon atoms, R21 - alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms;

[0088] Acylamidoalkylamine oxide with the general formula: R22-C(O)-NH-R23-N(-CH3)2-O, where R22 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms, R23 - an alkyl group with a long hydrocarbon chain from 1 to 4 carbon atoms;

[0089] Alkyl betaine with the general formula: R24-N(-CH3)2)-CH2-CO2, where R24 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms;

[0090] Alkylhydroxysultaine with the general formula: R25-N(-CH3)2-CH2-CH(-OH)-CH2-SO3, where R25 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;

[0091] Alkyl sultaine with the general formula: R26-N(-CH3)2-CH2-CH2-CH2-SO3, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms;

[0092] Alkylamine oxide with the general formula: R27-N(-CH3)2-O, where R26 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 6 to 22 carbon atoms.

[0093] Nonionic surfactants:

[0094] Alkyl glucoside with the general formula: R28-O-[G]p3, where R28 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 4 to 22 carbon atoms, G is a saccharide fragment containing 5 or 6 carbon atoms, p3 - can take values from 1 to 4;

[0095] Alkyl polyethylene glycol with the general formula: R29-O(-CH2-CH2-O-)n3H, where n3 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, R29 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms;

[0096] Alkyl polyethylene/propylene glycol with the general formula: R30-O(-CH2-CH2-O-)n4(-CH(-CH3)-CH2-O-)n5H, where n4 can range from 2 to 20, and represents the number of polyethylene glycol groups, n5 can take values from 2 to 20, and denotes the number of polypropylene glycol groups, R30 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms;

[0097] Dialkylpolyethylene glycol with the general formula: R31-O(-CH2-CH2-O-)n6R32, where n6 can take values from 2 to 20, and denotes the number of polyethylene glycol groups, R31 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, R32 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 1 to 12 carbon atoms;

[0098] Dialkyl polyethylene/propylene glycol with the general formula: R33-O(-CH2-CH2-O-)n7(-CH(-CH3)-CH2-O-)n8-R34, where n7 can range from 2 to 20, and denotes the number of polyethylene glycol groups, n8 can take values from 2 to 20, and denotes the number of polypropylene glycol groups, R33 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 6 to 22 carbon atoms, R34 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 1 to 12 carbon atoms.

[0099] Polysaccharide dispersion medium/solvent:

[0100] An organic alcohol with the general formula: R35(-OH)s1, where R35 is an alkyl group with a long hydrocarbon chain of 3 to 12 carbon atoms, S1 can take values from 1 to 12, and denotes the number of hydroxyl groups located in hydrocarbon radical in any order relative to each other;

[0101] Alkyl polypropylene glycol with the general formula: H(-CH(-CH3)-CH2-O-)n9R36, where n9 can take values from 2 to 10, and denotes the number of polypropylene glycol groups, R36 is an alkyl group with a hydrocarbon chain length of 1 up to 10 carbon atoms.

[0102] pH regulators:

[0103] Organic acids with the general formula: R37(-OH)s2(-COOH)m1, where R37 is an alkyl group with a long hydrocarbon chain from 1 to 12 carbon atoms, S2 can take values from 1 to 12, and denotes the number hydroxyl groups located in the hydrocarbon radical, in random order relative to each other, M1 - can take values from 1 to 4, and denotes the number of carboxyl groups located in the hydrocarbon radical, in random order relative to each other;

[0104] Solutions of alkali or alkaline earth metal hydroxides, ammonia, primary and tertiary alkylamines, primary and tertiary alkanolamines, primary and tertiary glucamines, basic amino acids, disodium citric acid, trisodium citric acid.

[0105] Chelating agent:

[0106] Methylglycine diacetic acid trisodium salt, glutamine diacetic acid tetrasodium salt, ethylenediamine (N,N)-disuccinate trisodium salt;

[0107] Organic acids, as well as alkali metal salts, ammonium, alkylammonium, alkanolammonium, glucoammonium, corresponding to these acids: citric acid, malic acid, tartaric acid, glutaric acid, adipic acid, glucuronic acid, galacturonic acid, galactaric acid, gluconic acid , phytic acid, polytaconic acid, polyacrylic acid, polymethacrylic acid, copolymer of acrylic and maleic acids, as well as organic acids with the general formula R38(-OH)s3(-COOH)m2, where R38 is an alkyl group with a long hydrocarbon chain of 1 up to 12 carbon atoms, S3 - can take values from 1 to 12, and denotes the number of hydroxyl groups located in the hydrocarbon radical, in random order relative to each other, M2 - can take values from 1 to 4, and denotes the number of carboxyl groups located in a hydrocarbon radical, in random order relative to each other.

[0108] Precipitation inhibitors:

[0109] Polysaccharide derivatives: sodium salt of carboxymethyl polysaccharide, hydroxyalkyl polysaccharide, alkyl polysaccharide;

[0110] Polyvinylpyrrolidone;

[0111] Water-soluble salts of polyacrylic acid, polymethacrylic acid, acrylic/methacrylic acid copolymer and maleic acid.

[0112] Defoamers:

[0113] Higher carboxylic acids with the general formula: R39-CO2H, where R39 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms;

[0114] Higher carboxylic alcohols with the general formula: R40-COH, where R40 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 5 to 21 carbon atoms;

[0115] Ethers of higher carboxylic alcohols with the general formula: R41-O-R42, where R41, R42 is an alkyl and/or alkenyl group with a long hydrocarbon chain of 4 to 22 carbon atoms;

[0116] Bisamides of alkyldiamines and higher carboxylic acids: with the general formula: R43-C(O)-NH-R44-NH-C(O)-R45, where R43, R45 is an alkyl and/or alkenyl group with a long hydrocarbon chain from 5 to 21 carbon atoms, and R44 is an alkyl radical with a hydrocarbon chain length of 1 to 12 carbon atoms;

[0117] Preservatives:

[0118] Organic acids and salts of alkali and alkaline earth metals, ammonium, alkylammonium, alkanolammonium, glucoammonium, corresponding to these acids: benzoic acid, sorbic acid, 4-methoxybenzoic acid, salicylic acid, undecylenic acid;

[0119] Organic alcohols and phenols: phenoxyethanol, benzyl alcohol, caprylyl glycol, ethylhexylglycerol, phenethyl alcohol, 3-methyl-4-isopropylphenol, 2,4-dichlorobenzyl alcohol;

[0120] Broad-spectrum biocides: benzisothiazolinone, dodecyldipropylene triamine;

[0121] Fungicides: sodium pyrithione, climbazole.

[0122] Enzymes: protease, amylase, pectate lyase, mannanase, cellulase, amino oxidase, nuclease, ferruloyl esterase, gluconase, tannase and other commercially available enzymes that are used in laundry, dishwashing, floor, glass, and all-purpose cleaners .

[0123] Oxygen-based bleaches: hydrogen peroxide, calcium peroxide, urea peroxide, ε-phthalimidoperoxycaproic acid and other commercially available components.

[0124] In general, the present invention can be characterized by the following features.

[0125] In one aspect, the invention relates to a composition for use in a household cleaning product consisting of lipase and β-cyclodextrin, wherein the weight ratio of lipase to β-cyclodextrin is (0.0025-0.25):(0.1 -1) respectively.

[0126] The composition may be characterized in that the amount of lipase in the specified weight ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075, 0. 01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0.25. The composition may differ in that the amount of β-cyclodextrin in the specified mass ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15, 0.2, 0 .25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85 , 0.9, 0.95 or 1.

[0127] The composition may differ in that said lipase is present in a water-glycerol solution.

[0128] The composition may be characterized in that said aqueous glycerol lipase solution is a commercially available product Lipex® Evity 200 L. The composition may be characterized in that said aqueous glycerol lipase solution is a commercially available product Lipex® Evity® 200 L, modified with additional glycerin. The composition may differ in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerin, and β-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% by weight. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. for the mass of the composition. The composition may differ in that in a composition containing Lipex® Evity® 200 L, modified with an additional amount of glycerin, the mass ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is (0.1-1):(0.5- 1.5) respectively.

[0129] The composition may differ in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.1, 0.15, 0.2, 0.25, 0 ,3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9 , 0.95 or 1. The composition may differ in that the amount of additional glycerin introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and additional glycerin introduced for modification is 0.5, 0.55, 0.6, 0, 65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5.

[0130] The composition may differ in that it additionally contains a decyl glucoside. The composition may differ in that said decyl glucoside is the commercially available product NaturalAPG HG0814CM. The composition may differ in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6):(10-14). The composition may differ in that the amount of Lipex® Evity® 200 L in the specified weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0.6. The composition may differ in that the amount of NaturalAPG HG0814CM in the stated weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5 or 14. Composition may differ in that the composition contains the commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerol, β-cyclodextrin and the commercially available product NaturalAPG HG0814CM, where the content of the commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, the content of the commercially available product NaturalAPG HG0814CM is 12% wt. for the mass of the composition.

[0131] The composition may be characterized in that the activity of said lipase is at least 40 LU/g or 40 LU/ml at pH 7.0.

[0132] The composition may differ in that said household chemical is selected from dishwashing detergent, floor and/or toilet cleaner, and/or sink and/or bathtub and/or glass cleaner, pipe cleaner, Laundry detergents, including delicates or baby laundry detergents, fabric softener, pre-wash and pre-wash stain remover, laundry gel and fabric softener.

[0133] In another aspect, the invention relates to a dishwashing detergent containing 0.0525-1% wt. compositions according to the invention.

[0134] In another aspect, the invention relates to a floor and/or toilet and/or sink and/or bathtub and/or glass cleaner containing 0.0525-1% wt. compositions according to the invention.

[0135] In another aspect, the invention relates to an all-purpose surface cleaner containing 0.0525-1% by weight of the composition of the invention.

[0136] In another aspect, the invention relates to a pipe cleaner containing 0.0525-2% wt. compositions according to the invention. The pipe cleaner may be characterized in that said product contains 0.0525-1.5% by weight of said composition, preferably 0.0525-1% by weight. the specified composition.

[0137] In another aspect, the invention relates to a laundry detergent containing 0.0525-1% wt. compositions according to the invention. The laundry detergent may be characterized in that the detergent is selected from a delicate laundry detergent and a baby laundry detergent. The laundry detergent may be characterized in that the laundry detergent is a powder laundry detergent.

[0138] In another aspect, the invention relates to the use of the composition of the invention to regulate the kinetics of enzymatic lipid breakdown and neutralize unpleasant odors on various surfaces, maintaining long-term cleanliness and a pleasant aroma. The application may differ in that said surface is selected from metal, polymer, enamel, glass or wood.

[0139] In another aspect, the invention relates to a method for preparing the composition according to the invention, including preliminary adding a solution of lipase to a dispersion of β-cyclodextrin, in a solvent, for example, water, and after adding the last portion of lipase, the dispersion is stirred for 10-15 minutes. This allows you to obtain a homogeneous solution.

EXPERIMENTAL PART

[0140] The examples included in this description are not limiting of the claimed invention and are provided only for the purpose of illustration and confirmation of the achievement of the expected technical results. These examples are among many experimental data obtained by the inventors that confirm the effectiveness of the agents within the scope of the invention.

[0141] Preclinical studies were conducted to evaluate the effectiveness of the composition of the invention.

Example 1.

[0142] A laboratory study was conducted on the cleaning performance of active ingredients, namely lipase and β-cyclodextrins, in detergents based on the same base.

[0143] As a base for incorporating the components of the complex, a mixture consisting of purified water, anionic surfactants 5-15%, nonionic surfactants based on glycosides <5%, glycerin, cotton extract, tetrasodium diacetate glutamate, monohydrate was used citric acid, sodium hydroxide, preservative and an aqueous solution of citric acid and silver citrate (Table No. 1).

[0144] The dishwashing detergent base was prepared as follows: the solubilizer glycerin and the chelating agent glutamate diacetate tetrasodium salt were mixed with purified water while stirring in an ordinary mixer until the mixture was homogeneous. Anionic surfactants were preliminarily homogenized with a sufficient amount of purified water when heated to 40-60°C. Next, nonionic surfactants based on glycosides and an anionic surfactant solution were added. Cotton extract and an aqueous solution of citric acid and silver citrate were also added. At the very end, an organic acid was added to regulate the pH, a preservative to maintain microbiological stability during shelf life. The ingredients were mixed until a homogeneous transparent solution was obtained. After mixing, the pH of the product was measured and adjusted by adding sodium hydroxide to the desired value.

[0145] The test method is based on OST 6-15-1662-90 “Household cleaning products”. The method for determining the washing ability for various surfaces is that the mass of removed artificial oil and fat contamination over a certain time was determined gravimetrically by the test product in relation to the comparison product. Glass plates were chosen as surfaces. The glass plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and wiped with ethyl alcohol. After preparation, the plates were weighed to determine the baseline. Next, a complex oil-fat contaminant was prepared, consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1: 4.9: 1.0 :5,1:7,3:2,1:4,5:20 respectively. Weighed amounts of lubricant and lanolin were stirred at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled and the yolk of a chicken egg and other components were added. The contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to a drying oven and calcined at 220±5°C for 8 minutes. Then cooled and weighed. The samples were used in their pure form. A standard household chemical with a similar quantitative composition was used as a means of comparison.

[0146] To carry out the tests, each plate was placed in a jar with a lid, a nylon cloth was placed on top and 1 jar with the plate was filled with a clean sample of the test product, another 1 jar with the plates was filled with 40 ml of the reference agent so that the ratio of water: lipophilic pollutant (fat) was maintained , oils, glycerides) such as 9:1. The jars were placed in a liquid shaker and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.

[0147] Based on the established data, the mass fraction of removed dirt was calculated. The mass fraction of the removed pollutant was calculated using the following formula:

Where

A is the mass of a piece of plate with contaminant before washing with the product, g;

B - mass of a piece of plate with contaminant on the plates after washing with the product, g.

B is the mass of the clean plate, g.

[0148] The parameter for the general assessment of the cleaning efficiency and the kinetics of the breakdown of lipid contaminants in the composition of the product was the calculation of the % of cleaning efficiency, determined by the laboratory assistant based on the results of the study. The indicator of the mass fraction of the removed pollutant allows one to evaluate the cleaning effectiveness against complex lipid-based contaminants when using the composition daily as part of a dishwashing detergent. The general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the detergent for washing dishes and various surfaces.

[0149] For quantitative data, the group arithmetic mean (M), standard deviation (SD), and standard error of the mean (SEM) were calculated. The obtained data were processed using MS Excel. The probability of differences in mean values at different time points was determined using Student's t-test under normal distribution in independent and dependent samples. Differences were considered significant at a significance level of p<0.05.

Results.

[0150] Based on the results of assessing the cleaning efficiency of the samples, it was established that the composition under study in the dishwashing detergent has a pronounced washing effect against complex lipid-protein contaminants when compared with a control product that does not contain components of the claimed composition.

[0151] At the end of the study, significant changes in the measured cleaning efficiency were observed. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, the efficiency of removing complex lipid-protein pollutants increased by 53.6% on average. The control version did not achieve 100%, which indicates the insufficient effectiveness of the surfactant combination for removing lipid-protein contaminants (Table No. 2).

[0152] Complex lipid-protein stains are difficult to remove from various surfaces due to fixation and insufficient action of surfactants.

[0153] The base of the dishwashing detergent composition will gently cleanse contaminants from the surfaces of dishes (glass, metal, polymer, ceramic) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition against complex lipid-protein contaminants, incl. outdated. A study of a dishwashing detergent with the claimed composition resulted in a pronounced synergistic effect against stains that are difficult to remove with conventional surfactant-based dishwashing detergents. The composition allows you to replace synthetic surfactants with surfactants of natural origin without losing the effectiveness of the product, which allows you to reduce the burden on the environment, maintain an approach to human health and provide a high percentage of natural ingredients in household chemicals for consumers.

[0154] Since the composition contains natural lipase obtained by biotechnology and natural β-cyclodextrins, a single use will ensure a high% removal of complex contaminants with low labor costs and saving time. Combining components in a composition allows you to regulate the kinetics of enzymatic breakdown of lipid substrates that fix complex contaminants. Lipase provides targeted cleavage of lipid contaminants in various phase systems, and cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, ensuring changes in the conformation of the protein domain with the active center and the correct orientation of substrates. Additionally, cyclodextrins bind released cleavage products that have an unpleasant odor, which makes it possible to neutralize odorous aromatic molecules and control the atmosphere in the room for human psychological comfort.

[0155] Thus, the combination of components makes it possible to achieve a synergistic effect against complex lipid-protein contaminants due to targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows regular use in household chemicals for various surfaces.

Example 2.

[0156] A laboratory study was conducted on the cleaning efficiency of the active ingredients, namely lipase and β-cyclodextrins. Purified water and glycerin were used as a base to include the components of the complex for the solubilization of β-cyclodextrins.

[0157] The test method is based on OST 6-15-1662-90 “Household cleaning products”. The method for determining the washing ability for various surfaces is to determine by gravimetric method the mass of removed artificial oil and fat contamination over a certain time by the test sample in relation to the comparison sample. Metal and plastic plates were chosen as surfaces. 8 metal and 8 plastic plates were thoroughly washed, rinsed and dried in an oven at 120°C for 60 minutes and wiped with ethyl alcohol. After preparation, the plates were weighed to determine the baseline. Next, a complex oil-fat contaminant was prepared, consisting of a lubricant based on leached industrial oil, lanolin, lecithin emulsifier, egg yolk, linseed oil, sunflower oil, oleic acid and distilled water in a ratio of 4.1: 4.9: 1.0 :5,1:7,3:2,1:4,5:20 respectively. Weighed amounts of lubricant and lanolin were stirred at room temperature, then heated in a water bath to 70°C and an emulsifier was added, the mixture was cooled and the yolk of a chicken egg and other components were added. The contaminant was applied to the plates with a pipette and left at room temperature for 30 minutes, after which it was transferred to a drying oven and calcined at 220±5°C for 8 minutes. Then cooled and weighed. The samples were used in their pure form. As a means of comparison, distilled water was used, which did not contain the claimed composition.

[0158] To carry out the tests, each plate was placed in a jar with a lid, nylon fabric was placed on top and 1 jar with the plate was filled with the test sample, another 1 jar with the plates was filled with 40 ml of the reference sample so that the ratio of water: lipophilic pollutant (fat, oils, glycerides) was 9:1. The jars were placed in a liquid shaker and shaken for 5 minutes. After shaking, the plates were washed in running water, then rinsed with distilled water and dried in an oven at 120°C for an hour, then cooled at room temperature and weighed.

[0159] Based on the established data, the mass fraction of removed dirt was calculated. The mass fraction of the removed pollutant was calculated using the following formula:

Where

A is the mass of a piece of plate with contaminant before washing with the sample, g;

B - mass of a piece of plate with contaminant on the plates after washing with the sample, g.

B is the mass of the clean plate, g.

[0160] The parameter for the general assessment of cleaning efficiency and the kinetics of breakdown of lipid contaminants in the composition of the product was the calculation of % cleaning efficiency, determined by the laboratory assistant based on the results of the study. The indicator of the mass fraction of the removed pollutant allows you to evaluate the cleaning effectiveness against complex lipid-based contaminants (oils and fats with fixing additives) when using the composition daily as part of a dishwashing detergent. The general positive trend in the use of dishwashing detergent with the claimed composition is an increase in the effectiveness of the detergent for washing dishes and various surfaces.

[0161] For quantitative data, the group arithmetic mean (M), standard deviation (SD), and standard error of the mean (SEM) were calculated. The obtained data were processed using MS Excel. The probability of differences in mean values at different time points was determined using Student's t-test under normal distribution in independent and dependent samples. Differences were considered significant at a significance level of p<0.05.

Results.

[0162] Based on the results of assessing the cleaning efficiency of the samples, it was found that the composition under study has a pronounced cleaning effect against complex lipid-protein contaminants on various surfaces when compared with a control sample that does not contain components of the claimed composition.

[0163] At the end of the study, pronounced changes in the measured cleaning efficiency on the hydrophilic stainless steel metal surface were observed. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, an increase in the content of components led to a statistically significant (p<0.05) increase in the removal of pollutants by the composition without a base with surfactants. At first, there is a logarithmic increase in efficiency along the curve, and then it reaches the stationary phase, when an increase in the content of components does not affect the increase in efficiency due to a shift in the equilibrium in the enzymatic reaction (Table No. 3). The rapid accumulation of reaction products leads to a chemical reaction constant K _equal and a shift in equilibrium towards the reverse reaction due to the concentration gradient, as well as changes in pH in the system.

[164] The change in cleaning efficiency on a hydrophobic polymer plastic surface is similar to a metal surface, however, contaminants are removed better even with a minimum percentage of composition input due to the physicochemical characteristics of the surface. According to the dynamics of the indicator, relative to the control sample without the components of the claimed composition, an increase in the content of components led to a statistically significant (p<0.05) increase in the removal of pollutants by the composition without a base with surfactants. At first, a logarithmic increase in efficiency along the curve is observed, and then it reaches the stationary phase, when an increase in the content of components does not affect the increase in efficiency due to a shift in the equilibrium in the enzymatic reaction (Table No. 4).

[165] The control version did not show effective removal of contaminants, which indicates the targeted action and high activity of the composition in a certain concentration range.

Table No. 3. Evaluation of cleaning efficiency on a metal surface

Table No. 4. Evaluation of cleaning efficiency on a polymer surface

[166] Complex lipid-protein stains are difficult to remove from various surfaces of the house due to the fixation and insufficient action of surfactants from household chemicals, despite their solubilizing properties.

[167] The base of the dishwashing detergent composition will gently cleanse contaminants from the surfaces of dishes (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition against complex lipid-protein contaminants , incl. outdated. A study of the cleaning efficiency of the composition showed a pronounced synergistic effect against contaminants that are difficult to remove using conventional dishwashing detergents based on surfactants of natural origin. The composition allows you to replace synthetic surfactants with surfactants of natural origin or reduce their content equivalently without losing the effectiveness of the product, which allows you to reduce the burden on the environment, maintain an approach to human health and provide a high percentage of natural ingredients in household chemicals for consumers.

[168] Since the composition contains lipase obtained by biotechnology and natural β-cyclodextrins, a single application will ensure a high percentage of removal of complex contaminants with low labor costs and saving time. Combining components in a composition allows you to regulate the kinetics of enzymatic breakdown of lipid substrates that fix complex contaminants. Lipase provides targeted cleavage of lipid contaminants in various phase systems, and cyclodextrins are cofactors for regulating enzyme activity and increasing affinity for triglycerides, ensuring changes in the conformation of the protein domain with the active center and the correct orientation of substrates. Additionally, cyclodextrins bind released cleavage products that have an unpleasant odor, which makes it possible to neutralize odorous aromatic molecules and control the atmosphere in the room for human psychological comfort.

[0169] Since the kinetics of the reaction is influenced by the reaction products, in particular the non-competitive influence of the released fatty acids on the allosteric centers of the lipase, β-cyclodextrins act as activity stabilizers and bind the reaction products from the system. The combined use of components retains high activity at different percentages of composition input, depending on the type of household chemical product and its composition.

[0170] Thus, the combination of components makes it possible to achieve a synergistic effect against complex lipid-protein contaminants due to targeted action and regulation of the kinetics of the triglyceride hydrolysis reaction, additionally neutralizing unpleasant odors, which allows for regular use in household chemicals for various surfaces.

Example 3.

[0171] A laboratory study was conducted on the deodorizing effectiveness of active components, namely lipase and β-cyclodextrins, and household chemicals containing these substances. Purified water and glycerin for the solubilization of β-cyclodextrins, as well as the mixture shown in Table No. 1, were used as a base to include the components of the complex.

[0172] The test procedure is based on the article Deodorizing Ability of Houttuynia cordata Thunb. (Dokudami) for Masking Garlic Odar - Hiromi Ikeura and allows you to evaluate the ability of the components to neutralize unpleasant odors using an organoleptic method.

[0173] Organic compounds of the group of thioesters R ₁ -SR ₂ and esters R ₃ -CO-OR ₄ from Allium sativum garlic fruits, obtained by ultrasonic extraction with 96% alcohol and filtration through a Schott funnel, were selected as unpleasant odors. Sterile medical containers made of polymer material were used as auxiliary materials. Using a pipette, 0.5 g of alcohol extract was applied strictly to the bottom of each polymer container and placed in a preheated drying cabinet (set temperature 60°C) for 15-30 minutes. Next, 1.0 g of purified water was poured into the bottom of the containers and left for 10 minutes. Afterwards, 0.8-1.0 g of the tested household chemical product was applied to the bottom of the glasses and mixed for 3-5 minutes using a vibration unit. After 3 minutes, each container is washed with 1 liter of warm running water of medium hardness to completely remove the liquid, and then dried at room temperature. The deodorizing effect was determined by an organoleptic method and assessed on a developed 5-point scale, where 0 points means it does not deodorize at all, and 5 points means it completely deodorizes.

[174] Samples of household chemicals were used in their pure form. As a means of comparison, we used distilled water, which did not contain the claimed composition, and a water-soluble zinc salt, which has a pronounced deodorizing effect due to the chemical structure of zinc ions Zn ²⁺ . To evaluate the result, a sensory group of 5 people was involved, since individual variability in the intensity of the aroma could be observed. For quantitative data, the average score obtained from the study was calculated.

Results.

[175] Based on the results of the study, it was established that the composition under study as part of household chemicals has a pronounced deodorizing effect compared to a comparison sample that does not contain the components of the claimed composition. Each of the components contributes to the overall effect, and their combination makes it possible to neutralize unpleasant odors up to 100%, depending on the concentration of substances according to the formula of the invention (Table No. 5). The synergistic effect of lipase and (3-cyclodextrins) is manifested in an increase in the effectiveness of eliminating unpleasant odor by +0.5 points, which is a good change when combining the components in addition to the effects described in examples 1 and 2.

Table No. 5. Evaluation of the deodorizing effect of the composition components

[0176] The composition base of a universal surface cleaner will gently cleanse dirt from surfaces (metal, polymer, glass, etc.) and increase the access of active components from the claimed composition to hard-to-reach areas, thereby increasing the effectiveness of the composition in neutralizing persistent unpleasant odors. A study of the deodorizing effect of the composition showed a pronounced synergistic effect against unpleasant odors that are difficult to mask or wash off using conventional surface cleaners based on surfactants of natural origin. The composition allows you to replace synthetic absorbers of unpleasant aroma, synthetic fragrances or aromatic compositions in the composition of the product without loss of effectiveness, which allows you to maintain a safe effect on human health, incl. people with allergic respiratory diseases, and provide a high percentage of natural ingredients in household chemicals for consumers.

[0177] Since the composition contains lipase obtained by biotechnology and natural β-cyclodextrins, a single use will provide a high% neutralization of unpleasant odors due to sulfur-containing components (mercaptans), organic acids, their esters and other compounds. Combining components in a composition allows you to specifically eliminate the cause of unpleasant odors, and not temporarily mask them with the help of aromatic substances with a more pronounced intensity of perception.

[0178] Thus, the combination of components makes it possible to achieve a synergistic effect against persistent unpleasant odors due to targeted action and high affinity for organic odor molecules, which makes it possible to regularly use them in household chemicals for various surfaces and control the atmosphere in the room for human psychological comfort .

Example 4.

[0179] Mix 9.00 g of glyceryl oleate, contained in various fats and vegetable oils, and 90.7 g of purified water to a homogeneous mixture when heated to 45°C and stirred at 200 rpm using a magnetic stirrer. After this, stop heating and cool to room temperature. In a separate container, a dispersion of 0.0027 g of lipase with an activity of >100 LU/g and 0.27 g of β-cyclodextrins, constituting 3% by weight of the lipophilic substrate, was prepared in 0.02 g of a mixture of dispersion medium (glycerol-sorbitol) in a ratio of 1 :1.4. Next, a water-glycerol solution of the components is introduced into the medium with the substrate with active mixing and incubated at 30°C for 4 hours. The initial pH of the solution was 8.20±0.10.

[0180] The degree of hydrolysis is determined by measuring pH due to the release of weak oleic acid having pKa(Acid)=4.8±0.1 [https://echa.europa.eu/registration-dossier/-/registered-dossier/12335/4 /22]. In particular, with a ratio of substrate and aqueous phase of 1:3333, a degree of hydrolysis of 76% was achieved in 270 minutes, which indicates the high cleaning efficiency of the composition. Example 5.

[0181] An instrumental physicochemical study was conducted to confirm the synergism of lipase and β-cyclodextrins when the composition is used together. Spectroscopy methods, in particular UV spectroscopy and fluorescence spectroscopy, are most suitable for describing the biological properties and interactions in organic molecules, in particular enzymes, polysaccharides, etc. Aromatic amino acids in lipase, such as tryptophan, tyrosine, phenylalanine and histidine , have absorption in the ultraviolet region of radiation (180-400 nm) and release fluorescence quanta. β-cyclodextrins interact with amino acid residues of lipase subunits, forming clathrate complexes on the surface of the enzyme, which changes the microenvironment of chromophore groups and thereby changes the ability to absorb and emit light quanta in different spectral regions.

[0182] For the study, lipase solutions of 2 g/l were prepared with a dispersion of P-cyclodextrins in a concentration from 0 to 4.54 g/l at pH 7.4, taking into account the technological aspect of obtaining the composition described in example 6.

[0183] For UV spectroscopy, absorption spectra were determined at a wavelength of 180-340 nm and the optical density of solutions A was calculated, indicating the absorption of lipase amino acids and the energetically favorable conformation of the enzyme for the enzymatic breakdown of triglycerides in lipid and complex protein-lipid contaminants. According to the results of the study, it was found that at an absorption maximum of 190-200 nm, the spectrum of lipase practically does not change, however, in the region of 270-290 nm there is an additional peak with a changed spectrum when adding a dispersion of β-cyclodextrins. The optical density of the peak at 270-290 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returned to its original value, indicating the formation of a stable clathrate complex between lipase and β-cyclodextrins. It is worth noting that the optimal ratio of lipase: cyclodextrins is from 1:15 to 1:40 without changing the effectiveness of the composition.

[0184] For fluorescence spectroscopy, the emission spectra of fluorescence quanta were determined at a wavelength of 280-450 nm and the relative fluorescence intensity F was calculated, indicating the energetically favorable conformation of the enzyme and the ability of amino acid residues to absorb and emit light quanta. According to the results of the study, it was found that with an absorption maximum of 350-360 nm, the emission spectrum does not change when adding a dispersion of β-cyclodextrins. The fluorescence intensity of the peak at 340-350 nm decreased with the addition of cyclodextrins to a concentration of 2.27 g/l, but then the optical density returned to its original value, indicating the formation of a stable clathrate complex between lipase and β-cyclodextrins. It is worth noting that the optimal ratio of lipase: cyclodextrins is from 1:15 to 1:40 without changing the effectiveness of the composition.

Example 6.

[0185] The method for producing the agent according to the invention includes the following steps.

Preparation of a dispersion of lipase and P-cyclodextrins.

[0186] The main process of preparing household chemicals is preceded by the stage of preliminary application of a lipase solution to a dispersion of β-cyclodextrins. β-cyclodextrin powder is added to the dispersion medium, the ratio of cyclodextrins to dispersion medium is 1:1.20-2.50. The process is carried out at room temperature and atmospheric pressure. Cyclodextrins are added in small portions with continuous stirring. After adding the last portion of cyclodextrins, the dispersion is stirred for 10-15 minutes, the signal for transition to the next stage of the process is the absence of large inclusions of cyclodextrins in the dispersion. Then, a lipase solution is added to the resulting dispersion in small portions, the ratio of cyclodextrins (by dry matter) to lipase solution is 1:0.5-1.50. After adding the last portion of lipase, the dispersion is stirred for 10-15 minutes.

Obtaining household chemicals containing this composition.

[0187] After settling, the resulting dispersion of lipase and β-cyclodextrins is dissolved in the main reactor to prepare a finished household chemical, for example, dishwashing detergent. The dispersion is pumped into preheated water, and the resulting solution is stirred until completely dissolved. If technically possible, a high-speed dispersant is used to intensify dissolution. After dissolution of the dispersion is completed, the following components specified in the description of the invention can be loaded into the reactor for preparing the finished product, according to the technical recipe.

Claims (24)

1. A composition intended for use in household chemicals, containing lipase and β-cyclodextrin, where the mass ratio of lipase and β-cyclodextrin is (0.0025-0.25):(0.1-1), respectively, and said lipase is in a water-glycerol solution, which is the commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerin. 2. The composition according to claim 1, characterized in that the amount of lipase in the specified mass ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075 , 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0 ,25. 3. The composition according to claim 1, characterized in that the amount of β-cyclodextrin in the specified mass ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15 , 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0 .8, 0.85, 0.9, 0.95 or 1. 4. The composition according to claim 1, characterized in that the composition contains Lipex® Evity® 200 L, modified with an additional amount of glycerin, and β-cyclodextrin, where the content of Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. for the mass of the composition. 5. The composition according to claim 1, characterized in that in the composition containing Lipex® Evity® 200 L, modified with an additional amount of glycerin, the mass ratio of Lipex® Evity® 200 L and glycerin additionally introduced for modification is (0.1-1) :(0.5-1.5) respectively. 6. The composition according to claim 5, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and glycerol additionally introduced for modification is 0.1, 0.15, 0.2, 0, 25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95 or 1. 7. The composition according to claim 5, characterized in that the amount of glycerin additionally introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and glycerin additionally introduced for modification is 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5. 8. The composition according to claim 1, characterized in that it additionally contains decyl glucoside. 9. The composition according to claim 8, characterized in that said decyl glucoside is a commercially available product NaturalAPG HG0814CM. 10. The composition according to claim 9, characterized in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6): (10-14). 11. Composition according to claim 10, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0 ,6. 12. The composition according to claim 10, characterized in that the amount of NaturalAPG HG0814CM in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13 .5 or 14. 13. A composition intended for use in household chemicals containing lipase, β-cyclodextrin and the commercially available product NaturalAPG HG0814CM, where the mass ratio of lipase and β-cyclodextrin is (0.0025-0.25):(0.1-1 ) respectively, where the specified lipase is in an aqueous-glycerol solution, which is a commercially available product Lipex® Evity® 200 L, modified with an additional amount of glycerol, where the content of the commercially available product Lipex® Evity® 200 L is 0.4% wt. by weight of the composition, the content of the additional amount of glycerol introduced for modification is 1% wt. by weight of the composition, where the content of the commercially available product NaturalAPG HG0814CM is 12% wt. for the mass of the composition. 14. Composition according to claim 13, characterized in that the amount of lipase in the specified mass ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.0025, 0.005, 0.0075 , 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.15, 0.2 or 0 ,25. 15. Composition according to claim 13, characterized in that the amount of β-cyclodextrin in the specified mass ratio of lipase and β-cyclodextrin (0.0025-0.25):(0.1-1) is 0.1, 0.15 , 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0 .8, 0.85, 0.9, 0.95 or 1. 16. The composition according to claim 13, characterized in that in the composition containing Lipex® Evity® 200 L, modified with an additional amount of glycerin, the mass ratio of Lipex® Evity® 200 L and glycerin additionally introduced for modification is (0.1-1) :(0.5-1.5) respectively. 17. The composition according to claim 16, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and glycerol additionally introduced for modification is 0.1, 0.15, 0.2, 0, 25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95 or 1. 18. The composition according to claim 16, characterized in that the amount of glycerin additionally introduced for modification in the specified mass ratio of Lipex® Evity® 200 L and glycerin additionally introduced for modification is 0.5, 0.55, 0.6, 0.65 , 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4 or 1.5. 19. The composition according to claim 13, characterized in that the weight ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is (0.2-0.6): (10-14). 20. The composition according to claim 19, characterized in that the amount of Lipex® Evity® 200 L in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 0.2, 0.3, 0.4, 0.5 or 0 ,6. 21. The composition according to claim 19, characterized in that the amount of NaturalAPG HG0814CM in the specified mass ratio of Lipex® Evity® 200 L and NaturalAPG HG0814CM is 10, 10.5, 11, 11.5, 12, 12.5, 13, 13 .5 or 14. 22. The composition according to claim 13, characterized in that the activity of said lipase is at least 40 LU/g or 40 LU/ml at pH 7.0. 23. Household chemicals for washing dishes, or for washing floors, or for washing a toilet, or for washing a sink, or for washing a bathtub, or for washing glass, or for washing surfaces, or for cleaning pipes, or for washing clothes, containing 0.0525-1% wt. composition according to any one of paragraphs. 1-22. 24. The use of a composition intended for use in household chemicals, according to any one of paragraphs. 1-22 or household chemicals according to item 23 to regulate the kinetics of enzymatic breakdown of lipids and neutralize unpleasant odors, maintain long-term cleanliness and a pleasant aroma.