WO2022209243A1 - 抗菌用鉄粉 - Google Patents
抗菌用鉄粉 Download PDFInfo
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- WO2022209243A1 WO2022209243A1 PCT/JP2022/003397 JP2022003397W WO2022209243A1 WO 2022209243 A1 WO2022209243 A1 WO 2022209243A1 JP 2022003397 W JP2022003397 W JP 2022003397W WO 2022209243 A1 WO2022209243 A1 WO 2022209243A1
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- antibacterial
- iron
- iron powder
- mass
- test
- Prior art date
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 173
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 145
- 229910052742 iron Inorganic materials 0.000 claims abstract description 61
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 27
- 229910052717 sulfur Inorganic materials 0.000 claims description 27
- 239000011593 sulfur Substances 0.000 claims description 27
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical group [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 23
- 229910052802 copper Inorganic materials 0.000 claims description 23
- 239000010949 copper Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 21
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 14
- 229910052698 phosphorus Inorganic materials 0.000 claims description 14
- 239000011574 phosphorus Substances 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 description 25
- -1 silver ions Chemical class 0.000 description 13
- 241000588724 Escherichia coli Species 0.000 description 12
- 241000191967 Staphylococcus aureus Species 0.000 description 12
- 230000000694 effects Effects 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 239000002245 particle Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000010828 elution Methods 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229910052709 silver Inorganic materials 0.000 description 4
- 239000004332 silver Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000010954 inorganic particle Substances 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- QPLDLSVMHZLSFG-UHFFFAOYSA-N Copper oxide Chemical compound [Cu]=O QPLDLSVMHZLSFG-UHFFFAOYSA-N 0.000 description 1
- 239000005751 Copper oxide Substances 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 238000005275 alloying Methods 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 239000013590 bulk material Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000011246 composite particle Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910000431 copper oxide Inorganic materials 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229910001447 ferric ion Inorganic materials 0.000 description 1
- 229910001448 ferrous ion Inorganic materials 0.000 description 1
- 238000009689 gas atomisation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002506 iron compounds Chemical class 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 238000009692 water atomization Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Definitions
- the present invention relates to antibacterial iron powder.
- Patent Document 1 proposes antibacterial composite particles containing antibacterial inorganic particles A having silver or silver ions and antibacterial inorganic particles B containing zinc, titanium, copper or nickel.
- the present invention has been made in view of such circumstances, and an object of the present invention is to provide an antibacterial iron powder that is inexpensive and has excellent antibacterial action.
- the antibacterial iron powder according to one aspect of the present invention contains metallic iron as a main component.
- the antibacterial iron powder according to one aspect of the present invention is inexpensive and has excellent antibacterial action.
- FIG. 1 to No. 4 is a graph showing the relationship between the elapsed time in 4 and the viable count of Staphylococcus aureus.
- FIG. 1 to No. 4 is a graph showing the relationship between the elapsed time in 4 and the number of viable E. coli bacteria.
- FIG. 16 to No. 21 is a graph showing the relationship between the elapsed time in No. 21 and the viable count of Staphylococcus aureus.
- FIG. 16 to No. 21 is a graph showing the relationship between the elapsed time in No. 21 and the number of viable E. coli bacteria.
- the antibacterial iron powder according to one aspect of the present invention contains metallic iron as a main component.
- the antibacterial iron powder is mainly composed of metallic iron, so it is inexpensive and has excellent antibacterial action.
- the antibacterial iron powder is a powder, it has a large surface area, and even if rust occurs on the surface of the metallic iron, it will naturally peel off, and the new surface of the metallic iron will continue to be exposed.
- it is easy to incorporate into various products and their materials that require antibacterial properties.
- the antibacterial iron powder further contains an antibacterial element, and that the metal iron contains the antibacterial element.
- the antibacterial action can be improved by including the antibacterial expression element contained in the metallic iron.
- the antibacterial expression element is sulfur or phosphorus.
- sulfur or phosphorus as the antibacterial element, the antibacterial effect can be remarkably improved.
- the sulfur content is preferably 0.02% by mass or more and 5% by mass or less. When the sulfur content is within the above range, the antibacterial effect can be easily and reliably improved.
- the phosphorus content is preferably 1% by mass or more and 5% by mass or less. When the phosphorus content is within the above range, the antibacterial effect can be easily and reliably improved.
- the antibacterial expression element may be copper. By using copper as the antibacterial element, the antibacterial effect can be easily and reliably improved.
- the antibacterial iron powder is preferably water atomized powder. Since the antibacterial iron powder is water-atomized powder, it is easy to increase the specific surface area compared to a bulk material such as an iron plate. As a result, it is easy to effectively exhibit an antibacterial action.
- the "main component” refers to a component with the highest content in terms of mass, for example, a component with a content of 50% by mass or more.
- antibacterial-exhibiting element includes an element that itself has antibacterial properties and an element that causes another element to exhibit antibacterial properties through a chemical reaction.
- the antibacterial iron powder is iron powder containing metallic iron as a main component.
- the metallic iron includes pure iron and iron compounds. Among them, pure iron is preferable as the metallic iron.
- the antibacterial iron powder is considered to exhibit an antibacterial effect by elution of divalent iron ions (Fe 2+ ). More specifically, ferrous ions are attracted to prokaryotes (bacteria) by electrostatic induction, reduce the cell wall of the prokaryote, and after penetrating into the cell wall, reduce the DNA within the cell wall, resulting in an antibacterial effect. It is thought that it plays an effect. At this time, if metallic iron contains impurities, the impurities may hinder the elution of divalent iron ions.
- the metallic iron is pure iron
- the divalent iron ions are appropriately eluted, and the desired antibacterial effect is likely to be exhibited.
- the lower limit of the content of pure iron in the metallic iron is preferably 50% by mass.
- the elution amount of divalent iron ions can be sufficiently increased, and the antibacterial action and the durability of the antibacterial action can be enhanced.
- the term “pure iron” means one that is readily available for industrial use and has a purity of 90.0% by mass or more.
- the lower limit of the average particle size of the antibacterial iron powder is preferably 50 ⁇ m, more preferably 60 ⁇ m.
- the upper limit of the average particle size is preferably 150 ⁇ m, more preferably 100 ⁇ m. If the average particle size is less than the lower limit, the manufacturing cost of the antibacterial iron powder may increase. Conversely, if the average particle size exceeds the upper limit, it may be difficult to sufficiently increase the specific surface area of the antibacterial iron powder, and thus it may be difficult to achieve a sufficient antibacterial effect.
- average particle size refers to the particle size distribution obtained by a dry sieving test using a sieve specified in JIS-Z8801-1: 2019, and the particle size at which the cumulative mass is 50% in this particle size distribution. .
- the antibacterial iron powder preferably contains an antibacterial expression element. Moreover, it is preferable that the antibacterial element is contained in the metallic iron.
- the antibacterial iron powder can improve the antibacterial action by containing the antibacterial element in the metallic iron.
- the "antibacterial expression element" is distinguished from the "metallic iron” as a separate component.
- antibacterial elements examples include sulfur (S) and phosphorus (P).
- the iron powder for antibacterial use contains sulfur or phosphorus in the metallic iron, so that the elution of divalent iron ions can be promoted.
- the antibacterial effect is further enhanced because sulfur itself is involved in the expression of the antibacterial action. More specifically, since the metallic iron contains sulfur, the elution of divalent iron ions can be promoted by the transfer of electrons from iron to sulfur. Furthermore, for example, when the antibacterial iron powder is placed in a liquid, sulfide ions (S 2 ⁇ ) are hydroxide ions (OH ⁇ ), sulfuric acid (H 2 SO 4 ), etc. Generating certain chemical species is thought to enhance the antibacterial activity.
- the lower limit of the sulfur content in the antibacterial iron powder is preferably 0.02% by mass, more preferably 0.3% by mass, and further 0.5% by mass. preferable.
- the upper limit of the content is preferably 5% by mass, more preferably 3% by mass, and even more preferably 2% by mass. If the content is less than the above lower limit, it may become difficult to exhibit the desired antibacterial improvement effect. Conversely, if the content exceeds the upper limit, it becomes difficult to incorporate sulfur into the antibacterial iron powder, and there is a risk that the manufacturing cost will be too high for the effect of improving the antibacterial action.
- the lower limit of the phosphorus content in the antibacterial iron powder is preferably 1% by mass, more preferably 1.5% by mass, and even more preferably 2% by mass.
- the upper limit of the content is preferably 5% by mass, more preferably 4% by mass, and even more preferably 3% by mass. If the content is less than the above lower limit, it may become difficult to exhibit the desired antibacterial improvement effect. Conversely, when the content exceeds the upper limit, it becomes difficult to incorporate phosphorus into the antibacterial iron powder, and there is a risk that the production cost will become too high for the effect of improving the antibacterial action.
- the antibacterial expression element may be copper. Copper is known to have an antibacterial effect, and has conventionally been used alone (copper alone) or as a mixed powder as described in Patent Document 1. In contrast, in the antibacterial iron powder, copper is alloyed with iron. In the antibacterial iron powder, copper, which has a lower ionization tendency than iron, is less likely to dissolve ions. It is considered that the behavior is different from that of copper alone or iron alone. In other words, the antibacterial iron powder is not based on the antibacterial action of copper itself, but is based on new findings that the antibacterial properties of iron can be activated by alloying copper and iron.
- an oxide film may be formed on the surface of the antibacterial iron powder, it is believed that this oxide film is likely to peel off because it is mainly composed of iron, unlike the copper oxide film formed on the surface of copper alone. As a result, it is presumed that the nascent surface of the iron part continues to be exposed, so that the antibacterial properties are likely to be sustained. Furthermore, since the antibacterial iron powder is alloyed with iron, the production cost can be kept lower than that of an antibacterial material made of copper alone.
- the lower limit of the copper content in the antibacterial iron powder is preferably 2% by mass, more preferably 3% by mass, and even more preferably 4% by mass.
- the upper limit of the content is preferably 10% by mass, more preferably 8% by mass, and even more preferably 6% by mass. If the content is less than the above lower limit, it may become difficult to exhibit the desired antibacterial improvement effect. Conversely, if the content exceeds the upper limit, it becomes difficult to add copper to the antibacterial iron powder, and there is a risk that the production cost will become too high for the effect of improving the antibacterial action.
- the manufacturing method of the antibacterial iron powder is not particularly limited.
- the antibacterial iron powder may be produced by, for example, a reduction method, a gas atomization method, or the like.
- the water atomization method is preferable as the method for producing the antibacterial iron powder. That is, the antibacterial iron powder is preferably water-atomized powder.
- Water-atomized powder is obtained by spraying high-pressure water onto molten metallic iron to make the metallic iron finer and solidified. Since the water-atomized powder has irregularities on its surface, it has a large specific surface area. Therefore, the water-atomized powder has excellent dissolution properties of divalent iron ions.
- the water-atomized powder is obtained by adding the antibacterial element to the metallic iron when the metallic iron is melted.
- the water-atomized powder prevents contamination with impurities (that is, selectively contains the antibacterial element) and facilitates control of the content of the antibacterial element. That is, according to the water-atomized powder, the composition of the entire antibacterial iron powder can be easily and reliably controlled. Therefore, since the antibacterial iron powder is a water-atomized powder, it can promote the elution of divalent iron ions, and can easily exhibit an effective antibacterial effect. Furthermore, since the antibacterial iron powder is water-atomized powder, the manufacturing cost can be reduced.
- the antibacterial iron powder can be used by blending it with products and materials such as miscellaneous goods, building materials, and furniture. That is, the antibacterial iron powder can be used by blending it with products and materials thereof that are in daily contact and for which propagation of bacteria is not desired from a sanitary point of view.
- the iron powder for antibacterial use is mainly composed of metallic iron, it is inexpensive and has excellent antibacterial action.
- the antibacterial iron powder is a powder, it has a large surface area, and even if rust occurs on the surface of the metallic iron, it will naturally peel off, and the new surface of the metallic iron will continue to be exposed.
- it is easy to incorporate into various products and their materials that require antibacterial properties.
- the antibacterial iron powder does not need to contain the antibacterial elements described above if it can exhibit an antibacterial effect by eluting divalent iron ions.
- Test Example 1 ⁇ Preparation of test bacterial solution> Using Staphylococcus aureus and Escherichia coli as test bacteria, each test bacteria was inoculated into a nutrient agar medium and cultured at a temperature of 30° C. or higher and 35° C. or lower for 24 hours. Thereafter, each test bacterium was prepared with physiological saline so that the number of bacteria was 10 8 [CFU (Colony Forming Unit)/mL] to prepare a test bacterium solution.
- CFU Coldy Forming Unit
- test sample> (No. 1 to No. 3) A water-atomized powder containing 1% by mass of sulfur in pure iron was used as a specimen. This sample was suspended in sterilized water to a concentration of 1 g/L, and 10 mL was dispensed into a test tube. 1 test sample. In addition, the specimen was suspended in the sterilized water to a concentration of 10 g/L, and 10 mL was dispensed into a test tube. No. 2 test sample was prepared by suspending the above specimen in the above sterilized water to a concentration of 100 g/L and dispensing 10 mL into a test tube. 3 test samples.
- Test Example 2 The above No. 1 to No. 3 sample and No. 3, which will be described later. 8 to No. Viable counts of Staphylococcus aureus and Escherichia coli were calculated in the same manner as in Test Example 1 using 15 specimens. No. 1 to No. 3 and No. 8 to No. For No. 15, each test was performed three times, and the average value in the three tests was obtained as the viable cell count. In addition, in Test Example 1 and Test Example 2, No. 1 to No. The calculation results of the viable count of the samples of 3 are different. This is considered to be an error caused by the test bacteria or the like. Table 2 shows the calculation results of the viable cell count.
- a water-atomized powder containing 0.3% by mass of sulfur in pure iron was used as a sample. This sample was suspended in sterilized water to a concentration of 1 g/L, and 10 mL was dispensed into a test tube. 8 test samples. In addition, the specimen was suspended in the sterilized water to a concentration of 10 g/L, and 10 mL was dispensed into a test tube. No. 9 test sample was suspended in the sterilized water to a concentration of 100 g/L, and 10 mL was dispensed into a test tube. There were 10 test samples.
- no. 1 to No. 3 can greatly reduce the number of viable bacteria for both Staphylococcus aureus and Escherichia coli. Further, No. 2 having a sulfur content of 0.02% by mass or more. 8 to No. Regarding No. 11, the number of viable bacteria decreased 4 hours after inoculation as compared to 1 hour after inoculation. Further, No. 1 having a sulfur content of 0.005% by mass. No. 12 also tends to have a lower viable cell count than the glass beads (No. 13 to No. 15) shown as comparative examples.
- the antibacterial iron powder can enhance the antibacterial action by containing sulfur in the metallic iron, and in particular, by setting the sulfur content to 0.02% by mass or more, viable bacteria It can be seen that the effect of reducing the number can be enhanced. This indicates that sulfur is an important antibacterial expression element.
- Test Example 3 A test bacterial solution was prepared in the same procedure as in Test Example 1, and the above-mentioned No. 1 to No. 3 sample and No. 3, which will be described later. 16 to No. Using the 21 specimens, the viable counts of Staphylococcus aureus and Escherichia coli were calculated in the same manner as in Test Example 1. Table 3 shows the results of calculating the number of viable bacteria. In addition, in Test Example 1 and Test Example 3, No. 1 to No. The calculation results of the viable count of the samples of 3 are different. This is considered to be an error caused by the test bacteria or the like. In addition, the viable cell count value "0" in Table 3 means that no bacteria were detected by culturing. In addition, the antibacterial expression element in Table 3 means an element that can obtain an antibacterial improvement effect by being contained in metallic iron, and the number of viable bacteria in Table 3 is the antibacterial effect of the antibacterial expression element alone. does not indicate
- a water-atomized powder containing 2% by mass of phosphorus in pure iron was used as a sample. This sample was suspended in sterilized water to a concentration of 1 g/L, and 10 mL was dispensed into a test tube. There were 16 test samples. In addition, the specimen was suspended in the sterilized water to a concentration of 10 g/L, and 10 mL was dispensed into a test tube. No. 17 test samples were prepared by suspending the above specimens in the above sterilized water at a concentration of 100 g/L and dispensing 10 mL into test tubes. There were 18 test samples.
- a water-atomized powder containing 5% by mass of copper in pure iron was used as a sample. This sample was suspended in sterilized water to a concentration of 1 g/L, and 10 mL was dispensed into a test tube. There were 19 test samples. In addition, the specimen was suspended in the sterilized water to a concentration of 10 g/L, and 10 mL was dispensed into a test tube. 20 test samples, the above specimens were suspended in the above sterilized water to a concentration of 100 g/L, and 10 mL was dispensed into test tubes. There were 21 test samples.
- the antibacterial iron powder can exert its antibacterial effect by being configured as, for example, an alloy powder containing copper.
- the antibacterial iron powder according to one aspect of the present invention is inexpensive and has excellent antibacterial action, so it can be suitably blended into various products and their materials.
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Abstract
Description
最初に本発明の実施態様を列記して説明する。
以下、適宜図面を参照しつつ、本発明の実施の形態を詳説する。なお、本明細書に記載されている数値については、記載された上限値と下限値とを任意に組み合わせることが可能である。本明細書では、組み合わせ可能な上限値から下限値までの数値範囲が好適な範囲として全て記載されているものとする。
当該抗菌用鉄粉は、金属鉄を主成分とする鉄粉である。上記金属鉄としては、純鉄及び鉄化合物が挙げられる。中でも、上記金属鉄としては、純鉄が好ましい。当該抗菌用鉄粉は、二価鉄イオン(Fe2+)の溶出によって抗菌作用を奏すると考えられる。より詳しくは、二価鉄イオンが静電誘導によって原核生物(細菌)に引き寄せられ、この原核生物の細胞壁を還元すると共に、この細胞壁内に浸透した後にこの細胞壁内のDNAを還元することで抗菌作用を奏すると考えられる。この際、金属鉄が不純物を含有していると、この不純物によって二価鉄イオンの溶出が妨げられる場合がある。これに対し、上記金属鉄が純鉄であることで、二価鉄イオンを適切に溶出させ、所望の抗菌作用を奏しやすい。上記金属鉄における純鉄の含有率の下限としては、50質量%が好ましい。上記純鉄の含有率が上記下限以上であることで、二価鉄イオンの溶出量を十分に大きくすることができ、抗菌作用及びこの抗菌作用の持続性を高めることができる。なお、「純鉄」とは、工業用として容易に入手が可能であり、純度が90.0質量%以上のものを意味する。
当該抗菌用鉄粉は、金属鉄を主成分としているので、安価で、かつ抗菌作用に優れる。また、当該抗菌性鉄粉は、粉体であるので、表面積が大きく、かつ金属鉄の表面に錆が生じても自然に剥落し、金属鉄の新生面が継続的に表出し続けるため、抗菌作用の持続性に優れると共に、抗菌性が要求される種々の製品及びその材料に対して配合しやすい。
上記実施形態は、本発明の構成を限定するものではない。従って、上記実施形態は、本明細書の記載及び技術常識に基づいて上記実施形態各部の構成要素の省略、置換又は追加が可能であり、それらは全て本発明の範囲に属するものと解釈されるべきである。
<試験菌液の調製>
黄色ブドウ球菌及び大腸菌をそれぞれ試験菌として用い、各試験菌を普通寒天培地に接種し、30℃以上35℃以下内の温度で24時間培養した。その後、各試験菌について、生理食塩水を用いて菌数が108[CFU(Colony Forming Unit)/mL]となるように調製し、試験菌液を作成した。
(No.1からNo.3)
純鉄に硫黄が1質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に1g/Lとなるように懸濁し、試験管に10mL分注したものをNo.1の試験試料とした。また、上記検体を上記滅菌水に10g/Lとなるように懸濁し、試験管に10mL分注したものをNo.2の試験試料とし、上記検体を上記滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.3の試験試料とした。
検体を懸濁していない滅菌水をNo.4の試験試料とした。
No.1からNo.4の試験試料にそれぞれ上述の試験菌液を0.1mL接種し、25℃で静置した。接種直後、接種から1時間後及び4時間後に、上記試験試料の10倍希釈系列をSCDLP液体培地(レシチン・ポリソルベート80添加ソイビーン・カゼイン・ダイジェスト液体培地)で調製して試験液を得た。これらの試験液をSCDLP寒天培地に接種し、30℃以上35℃以下の温度内で、72時間培養した。この培養後、形成された集落をカウントし、生菌数を算出した。No.1からNo.3については、それぞれ3回試験を行い、3回の試験における平均値を生菌数として求めた。この算出結果を表1に示す。なお、表1における生菌数の値「0」とは、培養により菌が検出されなかったことを意味する。
表1、並びに図1及び図2に示すように、純鉄に硫黄が含有されているNo.1からNo.3の検体では、黄色ブドウ球菌及び大腸菌共に、生菌数が大きく減少している。これは、硫黄が、二価鉄イオンの溶出を効果的に促進できているためと考えられる。なお、No.4に示すように、黄色ブドウ球菌と大腸菌とを比較した場合、黄色ブドウ球菌の生菌数については、接種から1時間後の生菌数よりも接種から4時間後の生菌数の方が増加している。これは、大腸菌と比較した際の試験菌種に起因する個体差が誤差として介在したためと考えられる。
上述のNo.1からNo.3の検体と、後述のNo.8からNo.15の検体とを用いて試験例1と同様の手順で黄色ブドウ球菌及び大腸菌の生菌数を算出した。No.1からNo.3及びNo.8からNo.15については、それぞれ3回試験を行い、3回の試験における平均値を生菌数として求めた。なお、試験例1と試験例2とでは、No.1からNo.3の検体の生菌数の算出結果が相違している。これは、試験菌等に起因する誤差であると考えられる。生菌数の算出結果を表2に示す。
純鉄に硫黄が0.3質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に1g/Lとなるように懸濁し、試験管に10mL分注したものをNo.8の試験試料とした。また、上記検体を上記滅菌水に10g/Lとなるように懸濁し、試験管に10mL分注したものをNo.9の試験試料とし、上記検体を上記滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.10の試験試料とした。
純鉄に硫黄が0.02質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.11の試験試料とした。
純鉄に硫黄が0.005質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.12の試験試料とした。
ガラスビーズを検体として用いた。この検体を滅菌水に1g/Lとなるように懸濁し、試験管に10mL分注したものをNo.13の試験試料とした。また、上記検体を上記滅菌水に10g/Lとなるように懸濁し、試験管に10mL分注したものをNo.14の試験試料とし、上記検体を上記滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.15の試験試料とした。
表2に示すように、硫黄の含有率が1質量%であるNo.1からNo.3は、黄色ブドウ球菌及び大腸菌共に、生菌数を大きく減少できている。また、硫黄の含有率が0.02質量%以上であるNo.8からNo.11についても、接種から4時間後の方が接種から1時間後よりも生菌数が減少している。また、硫黄の含有率が0.005質量%であるNо.12についても、比較例として示したガラスビーズ(Nо.13からNo.15)よりも生菌数は減少する傾向がみられる。このことから、当該抗菌用鉄粉は、金属鉄に硫黄が含有されていることで、抗菌作用を高めることができ、特に硫黄の含有率を0.02質量%以上とすることで、生菌数の減少効果を高められることが分かる。このことは、硫黄が重要な抗菌性発現元素であることを示している。
試験例1と同様の手順で試験菌液を作成し、上述のNo.1からNo.3の検体と、後述のNo.16からNo.21の検体とを用いて試験例1と同様の手順で黄色ブドウ球菌及び大腸菌の生菌数を算出した。生菌数の算出結果を表3に示す。なお、試験例1と試験例3とでは、No.1からNo.3の検体の生菌数の算出結果が相違している。これは、試験菌等に起因する誤差であると考えられる。また、表3における生菌数の値「0」とは、培養により菌が検出されなかったことを意味する。また、表3における抗菌性発現元素とは、金属鉄に含有されることで抗菌性向上効果が得られる元素を意味しており、表3における生菌数は、抗菌性発現元素単独による抗菌効果を示すものではない。
純鉄にリンが2質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に1g/Lとなるように懸濁し、試験管に10mL分注したものをNo.16の試験試料とした。また、上記検体を上記滅菌水に10g/Lとなるように懸濁し、試験管に10mL分注したものをNo.17の試験試料とし、上記検体を上記滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.18の試験試料とした。
純鉄に銅が5質量%の割合で含有されている水アトマイズ粉を検体として用いた。この検体を滅菌水に1g/Lとなるように懸濁し、試験管に10mL分注したものをNo.19の試験試料とした。また、上記検体を上記滅菌水に10g/Lとなるように懸濁し、試験管に10mL分注したものをNo.20の試験試料とし、上記検体を上記滅菌水に100g/Lとなるように懸濁し、試験管に10mL分注したものをNo.21の試験試料とした。
表3に示すように、硫黄の含有率が1質量%であるNo.1からNo.3は、黄色ブドウ球菌及び大腸菌共に、生菌数を大きく減少できている。また、表3、並びに図3及び図4に示すように、抗菌性発現元素として銅を用いたNo.19からNo.21については、黄色ブドウ球菌及び大腸菌のどちらにおいても生菌数が大きく減少する傾向が確認できる。さらに、表3、並びに図3及び図4に示すように、抗菌性発現元素としてリンを用いたNo.16からNo.18については、特に検体を滅菌水に100g/Lとなるように懸濁したNo.18において、黄色ブドウ球菌及び大腸菌のどちらにおいても生菌数が微減する傾向が確認できる。このことから、当該抗菌用鉄粉における抗菌性発現元素としては、硫黄の他に、リン及び銅も好ましいことが分かる。
Claims (7)
- 金属鉄を主成分とする抗菌用鉄粉。
- 抗菌性発現元素をさらに含んでおり、
上記抗菌性発現元素が上記金属鉄に含有されている請求項1に記載の抗菌用鉄粉。 - 上記抗菌性発現元素が硫黄又はリンである請求項2に記載の抗菌用鉄粉。
- 上記抗菌性発現元素が硫黄であり、上記硫黄の含有率が0.02質量%以上5質量%以下である請求項3に記載の抗菌用鉄粉。
- 上記抗菌性発現元素がリンであり、上記リンの含有率が1質量%以上5質量%以下である請求項3に記載の抗菌用鉄粉。
- 上記抗菌性発現元素が銅である請求項2に記載の抗菌用鉄粉。
- 水アトマイズ粉である請求項1から請求項6のいずれか1項に記載の抗菌用鉄粉。
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JP2019065375A (ja) * | 2017-09-29 | 2019-04-25 | 株式会社原田伸銅所 | 抗菌性および抗ウィルス性を有する銅合金粉体及びそれを用いた物品 |
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JP2005179607A (ja) | 2003-12-24 | 2005-07-07 | Fuji Xerox Co Ltd | 抗菌性複合粒子 |
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JPH08239726A (ja) * | 1995-03-01 | 1996-09-17 | Nippon Steel Corp | 抗菌、耐海生生物材料 |
JP2003064401A (ja) * | 2001-08-23 | 2003-03-05 | Kobe Steel Ltd | 抗菌・防かび性に優れたNi系粉末およびその製法、並びに該Ni系粉末を含む抗菌・防かび性に優れた材料、樹脂および部材 |
JP2011079798A (ja) * | 2009-10-09 | 2011-04-21 | Mitsubishi Materials Corp | 抗菌部材 |
JP2014508036A (ja) * | 2011-02-09 | 2014-04-03 | ホガナス アクチボラグ (パブル) | 流体を精製するための濾過媒体 |
JP2014045701A (ja) * | 2012-08-30 | 2014-03-17 | Jfe Steel Corp | 種子被覆材、該種子被覆材で被覆した被覆材被覆種子 |
JP2019065375A (ja) * | 2017-09-29 | 2019-04-25 | 株式会社原田伸銅所 | 抗菌性および抗ウィルス性を有する銅合金粉体及びそれを用いた物品 |
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