WO2022184150A1 - 一种全氟萘烷水凝胶促愈合敷料及其制备方法和用途 - Google Patents

一种全氟萘烷水凝胶促愈合敷料及其制备方法和用途 Download PDF

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WO2022184150A1
WO2022184150A1 PCT/CN2022/079138 CN2022079138W WO2022184150A1 WO 2022184150 A1 WO2022184150 A1 WO 2022184150A1 CN 2022079138 W CN2022079138 W CN 2022079138W WO 2022184150 A1 WO2022184150 A1 WO 2022184150A1
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perfluorodecalin
hydrogel
parts
dressing
healing
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French (fr)
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程宇豪
张忠涛
姚宏伟
杨开睿
杨沛峥
杨正阳
孟帅
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江苏君隽生物科技有限公司
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/008—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
    • A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
    • A61L26/0023—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0066—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/202—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with halogen atoms, e.g. triclosan, povidone-iodine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs

Definitions

  • the invention belongs to the technical field of hydrogels, and in particular relates to a perfluorodecalin hydrogel healing-promoting dressing and a preparation method and application thereof.
  • Topical oxygen therapy is a novel wound treatment technology that can deliver oxygen to wounds in a low-cost and portable manner.
  • perfluorodecalin is a very safe compound. It is a colorless, odorless, non-toxic transparent liquid at room temperature, with high density, low surface tension, stable chemical properties, and no metabolism in the body. What is particularly important is that perfluorodecalin has good gas carrying capacity, the solubility of oxygen is about 20 times that of water, 2 to 3 times that of whole blood, and the solubility of carbon dioxide is more than 3 times that of water. , antibacterial, antioxidant, anti-inflammatory and other effects. Perfluorodecalin has good histocompatibility and biological inertness, and has already been used in the field of biomedicine. However, due to its insoluble in water, poor stability, and low bioavailability, it has been The application on wound dressings has great difficulties.
  • hydrogel dressing that can actively supply oxygen and has a simple process to provide an oxygen-rich environment for wounds and solve the problem of slow healing of wound cells due to hypoxia.
  • the purpose of the present invention is to solve the shortcomings existing in the prior art, and propose a perfluorodecalin hydrogel healing-promoting dressing with simple process and controllable cost that can accelerate the healing of wounds and its preparation method and application .
  • a hydrogel healing-promoting dressing containing perfluorodecalin in parts by weight, comprises the following components: 100 parts of PBS buffer, 1-2 parts of sodium hyaluronate, 1-3 parts of fluorodecalin, 1-2 parts of stabilizer, 1-3 parts of emulsifier.
  • the emulsifier share Preferably, in parts by weight, it includes the following components: 100 parts of PBS buffer, 1.5-2 parts of sodium hyaluronate, 1.5-2 parts of perfluorodecalin, 1-1.5 parts of stabilizer, 2-3 parts of emulsifier share.
  • the molecular weight of the sodium hyaluronate is 200-600w
  • the stabilizer is xanthan gum
  • the emulsifier is any one of poloxamer, Tween-80 or Tween-20.
  • a preparation method of a hydrogel-promoting healing dressing comprising the following steps:
  • step b Add the perfluorodecalin liquid into the emulsifier-containing sodium hyaluronate hydrogel obtained in step a, stir at a constant speed at room temperature, and finally add a stabilizer and stir until uniform to obtain a perfluorodecalin-containing hydrogel Healing dressings.
  • the perfluorodecalin in the dressing increases the oxygen concentration and humidity on the wound surface through oxygen evolution, and can be applied to chronic wounds, trauma, burns, scalds, ulcers or Decubitus wounds.
  • the dressing is suitable for wounds on the frequently moving parts of the elbow, wrist, knee or ankle.
  • the dressing is suitable for chronic wounds.
  • the perfluorodecalin hydrogel dressing of the present invention is white and viscous, is a novel smart hydrogel wound dressing with both accelerated healing and safety, and can be applied to chronic wounds and trauma. , burns, scalds, ulcers, bedsores and other wounds, especially chronic wounds such as diabetic foot type are better.
  • the perfluorodecalin contained in the hydrogel dressing of the present invention has a strong ability to dissolve oxygen and good moisture and breathability. After smearing on the wound, it can isolate the wound and the external environment, so that the wound does not contact pathogens in the environment, which is conducive to maintaining a continuous oxygen supply environment for the wound, accelerating epithelial regeneration, and high safety; through oxygen evolution, the oxygen concentration on the wound surface can be effectively increased And humidity, the increase of oxygen concentration directly enhances wound healing, the increase of humidity is conducive to the penetration and diffusion of gas on the wound surface, and promotes wound healing through wet healing.
  • the emulsifier selected by the present invention has good wetting and dispersibility, and can disperse perfluorodecalin in the form of oil-in-water in the hydrogel, so that it can exist stably, overcoming the insolubility of perfluorodecalin
  • the stabilizer selected in the present invention has a good suspending effect on insoluble solids and oil droplets, and can form the hydrogel dressing into a jelly-like structure to form a stable gel form.
  • the present invention adopts the oil-in-water emulsion template method to prepare the perfluorodecalin hydrogel dressing; first, the emulsifier is dissolved in sodium hyaluronate as the water phase, and the perfluorodecalin is dispersed in the gel containing the emulsifier,
  • the oil-in-water emulsion system is formed by mixing, the preparation method is simple, the raw material sources are wide, the price is cheap, the cost is controllable, and industrial production can be realized.
  • the perfluorodecalin hydrogel dressing of the present invention has good mechanical properties, good flexibility and elasticity, can form good adhesion with the skin, and is suitable for wounds in different parts of the body, especially elbows, wrists, knees , ankles and other frequently active parts.
  • Fig. 1 is the treatment principle diagram of wound repair dressing of the present invention
  • Fig. 2 is the experimental figure of acute wound healing rate of the present invention
  • Fig. 3 is the chronic wound healing experiment diagram of the present invention.
  • Fig. 4 is the optical micrograph of the hydrogel sample of Example 1 of the present invention magnified by 400 times;
  • Fig. 5 is the optical micrograph of the 400-fold magnification of the hydrogel sample without perfluorodecalin according to the present invention
  • Fig. 7 is a shape diagram of a sample of the perfluorodecalin hydrogel dressing of the present invention.
  • Example 1 A hydrogel healing-promoting dressing containing perfluorodecalin, in parts by weight, comprising the following components: 100 parts of PBS buffer, 1 part of sodium hyaluronate with a molecular weight of 200w, perfluoronaphthalene 1 part of alkane, 1 part of xanthan gum, 1 part of poloxamer.
  • the preparation method of the hydrogel healing-promoting dressing includes the following steps: a. First, dissolving sodium hyaluronate in a PBS buffer, then adding an emulsifier and stirring at a uniform speed, and stirring at room temperature for 0.5 h to obtain a hyaluronate containing an emulsifier.
  • Sodium hydrogel b. Add perfluorodecalin liquid to the sodium hyaluronate hydrogel containing emulsifier obtained in step a, stir at a constant speed for 24 hours at room temperature, finally add stabilizer, stir manually for 20 minutes or rotate at 2000 rpm Magnetic stirring at 1/min until uniform, to obtain a hydrogel-promoting healing dressing containing perfluorodecalin.
  • Example 2 A hydrogel healing-promoting dressing containing perfluorodecalin, in parts by weight, comprising the following components: 100 parts of PBS buffer, 2 parts of sodium hyaluronate with a molecular weight of 200-600w, 3 parts of fluorodecalin, 2 parts of xanthan gum, 3 parts of Tween-80.
  • the preparation method of the hydrogel healing-promoting dressing includes the following steps: a. First, dissolving sodium hyaluronate in a PBS buffer, then adding an emulsifier and stirring at a constant speed, and stirring at room temperature for 1 hour to obtain hyaluronic acid containing an emulsifier Sodium hydrogel; b. Add perfluorodecalin liquid to the sodium hyaluronate hydrogel containing emulsifier obtained in step a, stir at a constant speed for 24h at room temperature, finally add stabilizer, stir manually for 20 minutes or rotate at 5000 rpm/ Magnetic stirring was carried out for 10 minutes until uniform, to obtain a hydrogel-promoting healing dressing containing perfluorodecalin.
  • Example 3 A hydrogel healing-promoting dressing containing perfluorodecalin, in parts by weight, comprising the following components: 100 parts of PBS buffer, 1.5 parts of sodium hyaluronate with a molecular weight of 200-600w, 1.5 parts of fluorodecalin, 1.2 parts of xanthan gum, 2 parts of Tween-80.
  • the preparation method of the hydrogel healing-promoting dressing includes the following steps: a. First, dissolving sodium hyaluronate in a PBS buffer, then adding an emulsifier and stirring at a constant speed, and stirring at room temperature for 0.8 h to obtain a hyaluronate containing an emulsifier.
  • Sodium hydrogel b. Add perfluorodecalin liquid to the sodium hyaluronate hydrogel containing emulsifier obtained in step a, stir at a constant speed for 24 hours at room temperature, and finally add stabilizer, stir manually for 20 minutes or rotate at 3000 rpm Magnetic stirring at 1/min until uniform, to obtain a hydrogel-promoting healing dressing containing perfluorodecalin.
  • Example 3 as an example to test the shape of the perfluorodecalin hydrogel dressing of the present invention
  • the blank control group was a non-diabetic group and healed naturally; the diabetic group was a diabetic mouse and healed naturally; the hydrogel group was a diabetic mouse and the wound was coated with a dressing without perfluorodecalin gel; perfluorodecalin water The gel group was diabetic mice and the wound was coated with the perfluorodecalin gel-containing dressing of Example 3 of the present invention.
  • the animal experiment method of the control hydrogel healing-promoting dressing includes the following steps: a. First raise the mice to adapt to the environment for 7 days, and the feed for the acute wound group is high-fat and high-sugar; b. Upper hydrogel dressing and control group reagent; c. Preparation of hydrogel without perfluorodecalin: first dissolve sodium hyaluronate in PBS buffer, then add emulsifier and stir at a constant speed, stir at a constant speed at room temperature for 24 hours, and finally The stabilizer was added and stirred by hand for 20 minutes or magnetically stirred at a rotational speed of 3000 rpm until uniform, to obtain a hydrogel dressing without perfluorodecalin. d. Change the dressing on time every day, measure and record the length of the wound on time, and obtain the wound healing curve, as shown in FIG. 2 .
  • Example 3 was compared with the above-mentioned comparative example in the acute wound healing rate experiment, as shown in Figure 2; and Example 3 was compared with the above-mentioned comparative example in the chronic wound healing rate experiment, as shown in Figure 3.
  • wound healing speed of the dressing of the present invention is obviously better than that of the gel product without perfluorodecalin and the natural healing speed.

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Abstract

本发明提供了一种含有全氟萘烷的水凝胶促愈合敷料，按重量份数计，包括以下组分：PBS缓冲液、透明质酸钠、全氟萘烷、稳定剂、乳化剂。制备方法，包括如下步骤：a、先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，得到含有乳化剂的透明质酸钠水凝胶；b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌，最后加入稳定剂，搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。本发明的全氟萘烷水凝胶敷料，是一种兼具加速愈合和安全性的新型智能水凝胶创面敷料，可应用于慢性伤口、外伤、烧伤、烫伤、溃疡、褥疮等伤口。

Description

一种全氟萘烷水凝胶促愈合敷料及其制备方法和用途 技术领域
本发明属于水凝胶技术领域，具体地涉及一种全氟萘烷水凝胶促愈合敷料及其制备方法和用途。
背景技术
随着人口逐渐老年化和糖尿病患者增加，慢性伤口已成为全球医疗领域面临的一个重大挑战；慢性伤口如糖尿病足等难以愈合的一个重要原因是，伤口部位局部低氧是限制伤口愈合的一个重要因素；目前，氧疗法主要分为两种，一类是高压氧疗法，一类是局部氧疗。局部氧疗法是一种新型的创面治疗技术，可以实现低成本、便携地往伤口输送氧气。
研究表明，全氟萘烷是一种安全性很高的化合物，常温下为无色、无味、无毒的透明液体,密度高,表面张力低,化学性质稳定,在体内不发生代谢。尤为重要的是,全氟萘烷具有良好的气体运载能力,对氧的溶解度约为水的20倍,是全血的2～3倍,二氧化碳的溶解度是水的3倍多；且具有创伤修复、抗菌性、抗氧化性、消炎等作用。全氟萘烷有良好的组织相容性和生物学上的惰性，早已在生物医用领域得到应用，但是由于全氟萘烷难溶于水，稳定性差，生物利用率低等特性，使其在创面敷料上的应用具有较大的困难。
因此，目前急需一种可以主动供氧且工艺简单的水凝胶敷料，为伤口提供富氧环境，解决创面细胞因缺氧而愈合缓慢的问题。
发明内容
本发明的目的是为了解决现有技术中存在的缺点，而提出的一种工艺简单、成本可控的能够加速愈合伤口的一种全氟萘烷水凝胶促愈合敷料及其制备方法 和用途。
本发明采用的技术方案为：一种含有全氟萘烷的水凝胶促愈合敷料，按重量份数计，包括以下组分：PBS缓冲液100份、透明质酸钠1-2份、全氟萘烷1-3份、稳定剂1-2份、乳化剂1-3份。
优选的，按重量份数计，包括以下组分：PBS缓冲液100份、透明质酸钠1.5-2份、全氟萘烷1.5-2份、稳定剂1-1.5份、乳化剂2-3份。
优选的，所述透明质酸钠的分子量为200-600w，所述稳定剂为黄原胶，所述乳化剂为泊洛沙姆、吐温-80或吐温-20中的任意一种。
水凝胶促愈合敷料的制备方法，包括如下步骤：
a、先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，在室温下搅拌0.5～1h，得到含有乳化剂的透明质酸钠水凝胶；
b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌，最后加入稳定剂，搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。
含有全氟萘烷的水凝胶促愈合敷料的用途，所述敷料中的全氟萘烷通过析氧提高伤口表面的氧气浓度和湿度，可应用于慢性伤口、外伤、烧伤、烫伤、溃疡或褥疮伤口。
优选的，所述敷料适用于手肘、手腕、膝盖或脚腕的经常性活动部位的伤口。
优选的，所述敷料适用于慢性伤口。
本发明获得的有益效果为：本发明的全氟萘烷水凝胶敷料，白色粘稠，是一种兼具加速愈合和安全性的新型智能水凝胶创面敷料，可应用于慢性伤口、 外伤、烧伤、烫伤、溃疡、褥疮等伤口，尤其是如糖尿病足式的慢性伤口效果更佳。
(1)本发明水凝胶敷料中含有的全氟萘烷具有强溶解氧的能力和良好的保湿透气性。涂抹于创面后，可隔绝创面和外环境，使伤口不接触环境中的病原体，有利于保持创面的持续供氧环境，加快上皮再生，安全性高；通过析氧可以有效提高伤口表面的氧气浓度和湿度，氧浓度的提高直接增效伤口愈合，湿度的提高有利于气体在伤口表面渗透扩散，以及通过湿性愈合的方式促进创面愈合。
(2)本发明选用的乳化剂具有良好的湿润分散性，能将全氟萘烷以水包油的形式分散在水凝胶之中，使其能够稳定存在，克服了全氟萘烷不溶于水、稳定性差的缺点，从而在涂抹伤口时能够缓慢地释放氧气。
(3)本发明选用的稳定剂对不溶性固体和油滴具有良好的悬浮作用，能将水凝胶敷料构成类似胶状结构，形成稳定的凝胶形态。
(4)本发明采用水包油乳液模板法制备全氟萘烷水凝胶敷料；先将乳化剂溶解于透明质酸钠作为水相，全氟萘烷分散于含有乳化剂的凝胶中，混合形成水包油乳液体系，制备方法简单，原料来源广泛、价格便宜，成本可控，可实现工业化生产。
(5)本发明的全氟萘烷水凝胶敷料具有良好的力学性能，柔韧性和弹性好，能够与皮肤形成良好粘合，适用于身体不同部位的创面，特别是手肘、手腕、膝盖、脚腕等经常性活动部位。
附图说明
图1为本发明伤口修复敷料的治疗原理图；
图2为本发明急性伤口愈合率实验图；
图3为本发明慢性伤口愈合实验图；
图4为本发明实施例1水凝胶样品放大400倍的光学显微图；
图5为本发明不含全氟萘烷水凝胶样品放大400倍的光学显微图；
图6为本发明水凝胶敷料样品涂抹于皮肤表面的照片；
图7为本发明全氟萘烷水凝胶敷料样品形状图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。
实施例1：一种含有全氟萘烷的水凝胶促愈合敷料，按重量份数计，包括以下组分：PBS缓冲液100份、分子量为200w的透明质酸钠1份、全氟萘烷1份、黄原胶1份、泊洛沙姆1份。
水凝胶促愈合敷料的制备方法，包括如下步骤：a、先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，在室温下搅拌0.5h，得到含有乳化剂的透明质酸钠水凝胶；b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌24h，最后加入稳定剂，手动搅拌20分钟或转速2000转/分钟下磁力搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。
实施例2：一种含有全氟萘烷的水凝胶促愈合敷料，按重量份数计，包括以下组分：PBS缓冲液100份、分子量为200-600w的透明质酸钠2份、全氟萘烷3份、黄原胶2份、吐温-80 3份。
水凝胶促愈合敷料的制备方法，包括如下步骤：a、先将透明质酸钠溶于PBS 缓冲液中，接着加入乳化剂匀速搅拌，在室温下搅拌1h，得到含有乳化剂的透明质酸钠水凝胶；b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌24h，最后加入稳定剂，手动搅拌20分钟或转速5000转/分钟下磁力搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。
实施例3：一种含有全氟萘烷的水凝胶促愈合敷料，按重量份数计，包括以下组分：PBS缓冲液100份、分子量为200-600w的透明质酸钠1.5份、全氟萘烷1.5份、黄原胶1.2份、吐温-80 2份。
水凝胶促愈合敷料的制备方法，包括如下步骤：a、先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，在室温下搅拌0.8h，得到含有乳化剂的透明质酸钠水凝胶；b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌24h，最后加入稳定剂，手动搅拌20分钟或转速3000转/分钟下磁力搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。
以实施例3为例对本发明全氟萘烷水凝胶敷料的形状进行测试
空白对照组为非糖尿病组且自然愈合；糖尿病组为患糖尿病的小鼠且自然愈合；水凝胶组为患糖尿病小鼠且伤口涂覆不含全氟萘烷凝胶的敷料；全氟萘烷水凝胶组为患糖尿病小鼠且伤口涂覆本发明实施例3含全氟萘烷凝胶的敷料。
对照水凝胶促愈合敷料的动物实验方法，包括如下步骤：a、先养老鼠适应环境7天，急性伤口组饲料为高脂高糖；b、在老鼠背部剪出直径1cm左右的伤口，涂上水凝胶敷料与对照组试剂；c、不含全氟萘烷的水凝胶制备：先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，室温匀速搅拌24h，最后加入稳定剂，手动搅拌20分钟或转速3000转/分钟下磁力搅拌至均匀，即得不含全氟萘烷的水凝胶敷料。d、每天准时换药，准时测量伤口长度并记录， 制得伤口愈合曲线，如图2所示。
将实施例3与上述对比例进行急性伤口愈合率实验对比，如图2所示；并将实施例3与上述对比例进行慢性伤口愈合率实验对比，如图3所示。
实验表明，本发明敷料的伤口愈合速度明显优于无含全氟萘烷凝胶品及自然愈合的速度。
以上所述，仅为本发明较佳的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，根据本发明的技术方案及其发明构思加以等同替换或改变，都应涵盖在本发明的保护范围之内.

Claims (7)

  1. 一种含有全氟萘烷的水凝胶促愈合敷料，其特征在于：按重量份数计，包括以下组分：PBS缓冲液100份、透明质酸钠1-2份、全氟萘烷1-3份、稳定剂1-2份、乳化剂1-3份。
  2. 根据权利要求1所述一种含有全氟萘烷的水凝胶促愈合敷料，其特征在于：按重量份数计，包括以下组分：PBS缓冲液100份、透明质酸钠1.5-2份、全氟萘烷1.5-2份、稳定剂1-1.5份、乳化剂2-3份。
  3. 根据权利要求1或2所述一种含有全氟萘烷的水凝胶促愈合敷料，其特征在于：所述透明质酸钠的分子量为200-600w，所述稳定剂为黄原胶，所述乳化剂为泊洛沙姆、吐温-80或吐温-20中的任意一种。
  4. 水凝胶促愈合敷料的制备方法，其特征在于：包括如下步骤：
    a、先将透明质酸钠溶于PBS缓冲液中，接着加入乳化剂匀速搅拌，在室温下搅拌0.5～1h，得到含有乳化剂的透明质酸钠水凝胶；
    b、将全氟萘烷液体加入步骤a得到的含有乳化剂的透明质酸钠水凝胶中，室温匀速搅拌，最后加入稳定剂，搅拌至均匀，即得含全氟萘烷的水凝胶促愈合敷料。
  5. 含有全氟萘烷的水凝胶促愈合敷料的用途，其特征在于：所述敷料中的全氟萘烷通过析氧提高伤口表面的氧气浓度和湿度，可应用于慢性伤口、外伤、烧伤、烫伤、溃疡或褥疮伤口。
  6. 根据权利要求5所述含有全氟萘烷的水凝胶促愈合敷料的用途，其特征在于：所述敷料适用于手肘、手腕、膝盖或脚腕的经常性活动部位的伤口。
  7. 根据权利要求5所述含有全氟萘烷的水凝胶促愈合敷料的用途，其特征在于：所述敷料适用于慢性伤口。
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