WO2022184050A1 - 用于生物组织冷冻保存或解冻复苏的生物芯片 - Google Patents

用于生物组织冷冻保存或解冻复苏的生物芯片 Download PDF

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Publication number
WO2022184050A1
WO2022184050A1 PCT/CN2022/078601 CN2022078601W WO2022184050A1 WO 2022184050 A1 WO2022184050 A1 WO 2022184050A1 CN 2022078601 W CN2022078601 W CN 2022078601W WO 2022184050 A1 WO2022184050 A1 WO 2022184050A1
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Prior art keywords
biochip
thawing
carrier
hydrogel
solution
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PCT/CN2022/078601
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English (en)
French (fr)
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陈昊楠
舒怡玮
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深圳拜尔洛克生物技术有限公司
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Application filed by 深圳拜尔洛克生物技术有限公司 filed Critical 深圳拜尔洛克生物技术有限公司
Priority to JP2023553317A priority Critical patent/JP2024508150A/ja
Priority to EP22762515.9A priority patent/EP4302601A1/en
Priority to US18/279,639 priority patent/US20240138400A1/en
Priority to AU2022229462A priority patent/AU2022229462A1/en
Priority to BR112023017774A priority patent/BR112023017774A2/pt
Publication of WO2022184050A1 publication Critical patent/WO2022184050A1/zh

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/50—Cryostats

Definitions

  • the utility model relates to the technical field of biological engineering, in particular to a biological chip used for cryopreservation or thawing recovery of biological tissues.
  • Cell cryopreservation technology is an important technology in many biological fields. It is also important to restore cells from a cryogenic frozen state to a normal metabolic state. This thawing and recovery process includes the removal of cryoprotectants and replacement of normal media. process.
  • the devices used in embryo freezing or thawing in the prior art have problems such as complicated operation and low efficiency.
  • the main purpose of the present utility model is to provide a biological chip for cryopreservation or thawing recovery of biological tissues, so as to solve the problems of complicated operation and low efficiency in the prior art device during embryo freezing or thawing.
  • a biochip for cryopreservation or thawing recovery of biological tissue wherein the four ends of the biochip are closed structures; the biochip includes: an interval area arranged in the middle position, the interval The area includes a plurality of intervals arranged in sequence, and partition plates are arranged between the intervals; a fixing groove is arranged at the bottom of each interval; and the joint parts are arranged on both sides of the interval area.
  • the bottom of the section area is further provided with a sealing film, and the sealing film forms the plurality of sections into a plurality of grooves.
  • the bottom of the interval area is further provided with a permeable film, and the permeable film forms the plurality of intervals into a plurality of grooves.
  • one end of the biochip has one or two chamfers or bevels.
  • a transparent baffle is further arranged above the biochip, and the transparent baffle covers the middle part of the interval area.
  • the interval area is provided with 2, 3 or 4 intervals.
  • the device by providing a biochip with a four-end closed structure, multiple sections of the biochip can encapsulate hydrogel or solution, the device is simple to operate, and can effectively improve the efficiency of embryo cryopreservation or thawing recovery.
  • FIG. 1 is a schematic diagram of a biochip with 2 sections according to an embodiment of the present invention.
  • Fig. 2 is the schematic diagram of the cross section of Fig. 1;
  • FIG. 3 is a schematic diagram of a biochip with three sections according to an embodiment of the present invention.
  • Fig. 4 is the schematic diagram showing arc surface in Fig. 2;
  • FIG. 5 is a schematic diagram of a biochip including a transparent baffle according to an embodiment of the present invention.
  • FIG. 6 is a schematic perspective view of a biochip including a transparent baffle according to an embodiment of the present invention.
  • Fig. 7 is the schematic diagram of the cross section of Fig. 5;
  • FIG. 8 is a schematic diagram showing the arc surface in FIG. 7 .
  • the biochip according to the embodiment of the present application has a generally rectangular parallelepiped structure, and the biochip mainly includes: an interval area arranged in the middle position and joint parts 12 arranged on both sides of the interval area. Portion 12 can be used to place a magnet or slot in conjunction with a pushing device.
  • the biochip adopts a four-end closed structure. In practical applications, the embryo is placed in a carrier groove, and more culture medium is added to fill the groove and a bulging culture liquid droplet appears above the groove.
  • one or two chamfers or inclined surfaces 15 may be provided at one end of the biochip.
  • the chamfer or slope 15 is used to indicate the correct orientation of the biochip, that is, the end with the chamfer or slope faces the embryo, so that the section close to the chamfer or slope first contacts the embryo, and the section far from the slope then contacts the embryo.
  • the section area includes a plurality of sections 11 arranged in sequence.
  • a partition plate is provided between adjacent sections, and the partition plate divides the section area into mutually independent sections.
  • the compartments are used to seal different types of hydrogels or solutions, and the number of compartments required varies in different usage environments.
  • the biochip in a freezing application, as shown in FIG. 1 , the biochip includes 2 sections; in a thawing application, as shown in FIG. 3 , the biochip includes 3 sections.
  • 4 intervals can be set, and the extra interval can be used to seal solutions of different concentrations, so as to slow down the change of osmotic pressure and make the embryo suffer less osmotic pressure damage.
  • the biochip according to the embodiment of the present application is used to encapsulate the chemicals put in it, and the chemicals can be water-coagulated chemicals. Solid form made in gel form or solution form.
  • the biochip includes 2 compartments that encapsulate the equilibrated fluid (ES) hydrogel and the vitrified fluid (VS) hydrogel, respectively. Specifically, a sealing film is attached to the bottom of the biochip, and the section of the biochip is formed into two grooves. The ES hydrogel solution and the VS hydrogel solution were filled into two grooves respectively, and a sealing film was attached to the top of the biochip after the hydrogel was cured. At this point, the hydrogels in each section are separated and sealed to increase the storage time.
  • ES equilibrated fluid
  • VS vitrified fluid
  • Both sides of the bottom of each section are respectively provided with fixing grooves 13, and the fixing grooves 13 are used to fix the position of the hydrogel after curing, so as to avoid pulling the hydrogel out of the section when the sealing film is torn off.
  • There are two inclined planes at one end of the biochip to indicate the correct orientation of the biochip that is, the section containing ES hydrogel near the inclined plane first contacts the embryo, and the section containing VS hydrogel far from the inclined plane contact with the embryo.
  • direction marks (such as arrows) can also be printed on the sealing film to indicate the correct orientation of the biochip.
  • the biochip includes 3 compartments that encapsulate thawing solution (TS) hydrogel, diluent (DS) hydrogel, and cleaning solution (WS) hydrogel, respectively glue.
  • TS thawing solution
  • DS diluent
  • WS cleaning solution
  • a sealing film is attached to the bottom of the biochip, and the section of the biochip is formed into three grooves.
  • the TS, DS and WS hydrogel solutions were filled into 3 grooves respectively, and a sealing film was attached to the top of the biochip after the hydrogel was cured. At this point, the hydrogels in each section are separated and sealed to increase the storage time.
  • Fixing grooves 13 are respectively provided on both sides of the bottom of each section, and the fixing grooves 13 are used to fix the position of the hydrogel after curing, so as to avoid pulling the hydrogel out of the section when the sealing film is torn off.
  • One end of the biochip is provided with two inclined planes, which are used to indicate the correct orientation of the biochip, that is, the section containing the TS hydrogel near the inclined plane is in contact with the embryo first, and the section containing the DS hydrogel in the middle is then in contact with the embryo. In contact, the section containing the WS hydrogel away from the slant finally made contact with the embryo.
  • direction marks (such as arrows) can also be printed on the sealing film to indicate the correct orientation of the biochip.
  • the carrier When thawing, remove the carrier from liquid nitrogen and put it into the oil phase thawing solution that has been preheated to 37 degrees Celsius for thawing and rewarming. After the carrier is thawed and rewarmed, the upper and lower sealing films of the biochip are torn apart, and the biochip is placed in the indicated direction so that the biochip is closely attached to the top of the carrier. Then, the biochip is moved relative to the carrier, and the TS, DS and WS hydrogels in the biochip area contact the embryos in the carrier groove in sequence, and the cryoprotectant in the embryos is removed. After the removal is complete, the biochip is detached from the carrier, and the embryos are recovered from the carrier groove using a glass capillary into a petri dish for culture.
  • the biochip includes 2 compartments encapsulating ES solution and VS solution, respectively. Specifically, a permeable film is first attached to the bottom of the biochip, and a sealing film is attached to the top of the biochip, so that the interval of the biochip is formed into two grooves.
  • One end of the biochip is provided with 2 inclined planes to indicate the correct orientation of the biochip, that is, the area close to the inclined plane will be added with ES solution to contact the embryo first, and the area away from the inclined plane will be added with VS when in use.
  • the solution is then contacted with the embryos.
  • direction indications eg arrows
  • the biochip includes 3 compartments that encapsulate the TS solution, the DS solution, and the WS solution, respectively. Specifically, a permeable film is first pasted on the bottom of the biochip, and a sealing film is pasted on the top of the biochip, so that the interval of the biochip is formed into three grooves.
  • One end of the biochip is provided with two inclined planes to indicate the correct orientation of the biochip, that is, the area close to the inclined plane will be added with TS solution to contact the embryo first, and the middle area will be added with DS solution during use. Embryos are in contact, and WS solution will be added to the area away from the inclined plane to finally contact the embryos.
  • direction marks can also be printed on the sealing film to indicate the correct orientation of the biochip.
  • thawing remove the carrier from liquid nitrogen and put it into the oil phase thawing solution that has been preheated to 37 degrees Celsius for thawing and rewarming. After the carrier is thawed and rewarmed, tear off the sealing film on the top of the biochip, place the biochip in the indicated direction, and stick the biochip on the carrier. Add the required solutions (such as TS, DS and WS) to the corresponding biochip section, and then move the biochip relative to the carrier.
  • required solutions such as TS, DS and WS
  • the TS, DS and WS solutions in the biochip section will pass through the permeable membrane through diffusion, in order
  • the embryos in the carrier groove are contacted in turn, and the cryoprotectant in the carrier groove and the embryo is diffused into the solution of the biochip, so as to achieve the purpose of removing the cryoprotectant in the embryo.
  • the biochip is detached from the carrier, and the embryos are recovered from the carrier groove using a glass capillary into a petri dish for culture.
  • a transparent baffle 18 is provided above the biochip, and the transparent baffle covers the middle part of the interval area.
  • the sealing film is attached to the bottom of the biochip, the area of the biochip forms a structure with holes on both sides and a hollow in the middle.
  • the hydrogel solution when using hydrogel for freezing or thawing, can be filled into the interior of the interval from one of the holes on both sides of the transparent baffle. Due to the baffle, the solution will fill the entire area.
  • the hollow structure does not form a curved surface under the baffle, and the curved surface 19 only appears on the edges of the holes on both sides of the baffle, which does not affect the optical path of using a microscope to observe the embryos in the carrier groove during use.
  • a sealing film can be applied on top of the biochip.

Abstract

一种用于生物组织冷冻保存或解冻复苏的生物芯片，所述生物芯片的四端为封闭结构；所述生物芯片包括：设置在中间位置的区间区域，所述区间区域包括多个依次排列的区间（11），所述区间（11）之间设置有分隔板；每个区间（11）的底部设置有固定槽（13）；接合部（12），其设置在所述区间区域的两侧。上述四端为封闭结构的生物芯片，生物芯片的多个区间能够封装水凝胶或溶液，使得装置操作简单，能够有效提高胚胎冷冻保存或解冻复苏的效率。

Description

用于生物组织冷冻保存或解冻复苏的生物芯片 技术领域
本实用新型涉及生物工程技术领域，尤其涉及一种用于生物组织冷冻保存或解冻复苏的生物芯片。
背景技术
细胞冷冻保存技术在很多生物领域是一项重要的技术，把细胞从低温冷冻状态恢复到正常代谢状态也同样重要，这个解冻复苏的过程都包含了把冷冻保护剂移除替换成正常培养基的过程。
现有技术在胚胎冷冻或解冻时使用的装置具有操作复杂，效率较低等问题。
实用新型内容
本实用新型的主要目的在于提供一种用于生物组织冷冻保存或解冻复苏的生物芯片，以解决现有技术的装置在进行胚胎冷冻或解冻时存在的操作复杂、效率较低等问题。
根据本实用新型实施例提出一种用于生物组织冷冻保存或解冻复苏的生物芯片，所述生物芯片的四端为封闭结构；所述生物芯片包括：设置在中间位置的区间区域，所述区间区域包括多个依次排列的区间，所述区间之间设置有分隔板；每个区间的底部设置有固定槽；接合部，其设置在所述区间区域的两侧。
其中，所述区间区域的底部还设置有密封膜，所述密封膜将所述多个区间形成为多个凹槽。
其中，所述密封膜上具有方向标识。
其中，所述区间区域的底部还设置有通透性薄膜，所述通透性薄膜将所述多个区间形成为多个凹槽。
其中，所述生物芯片的一端具有一个或2个倒角或斜面。
其中，所述生物芯片的上方还设置有透明挡板，所述透明挡板覆盖所述区间区域的中间部分。
其中，所述区间区域设置有2个、3个或4个区间。
根据本实用新型的技术方案，通过提供四端封闭结构的生物芯片，生物芯片的多个区间能够封装水凝胶或溶液，本装置操作简单，能够有效提高胚胎冷冻保存或解冻复苏的效率。
附图说明
此处所说明的附图用来提供对本实用新型的进一步理解，构成本申请的一部分，本实用新型的示意性实施例及其说明用于解释本实用新型，并不构成对本实用新型的不当限定。在附图中：
图1是根据本实用新型实施例的2区间的生物芯片的示意图；
图2是图1的剖面的示意图；
图3是根据本实用新型实施例的3区间的生物芯片的示意图；
图4是在图2中示出弧面的示意图；
图5是根据本实用新型实施例的包括透明挡板的生物芯片的示意图；
图6是根据本实用新型实施例的包括透明挡板的生物芯片的立体示意图；
图7是图5的剖面的示意图；
图8是在图7中示出弧面的示意图。
具体实施方式
为使本实用新型的目的、技术方案和优点更加清楚，下面将结合本实用新型具体实施例及相应的附图对本实用新型技术方案进行清楚、完整地描述。显然，所描述的实施例仅是本实用新型一部分实施例，而不是全部的实施例。基于本实用新型中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本实用新型保护的范围。
以下结合附图，详细说明本实用新型各实施例提供的技术方案。
参考图1至图3，根据本申请实施例的生物芯片大体为长方体结构，所述生物芯片主要包括：设置在中间位置的区间区域以及设置在所述区间区域的两侧的接合部12，接合部12可用于放置磁铁或跟推动设备结合的槽。所述生物芯片采用四端封闭的结构，在实际应用中，胚胎被放置在一个载体凹槽内，多加培养液填满凹槽并让凹槽的上方出现一个鼓起来的培养液液滴。由于有过剩的培养液在凹槽的上方，当采用封闭结构的生物芯片时，生物芯片一接触到过剩的培养液，(由于毛细作用)生物芯片和载体之间的细缝就会被过剩的培养液填满。细缝被填满后，就不会影响后面到来的胚胎。
在本申请实施例中，可在生物芯片的一端设置1个或2个倒角或斜面15。该倒角或斜面15用于表示生物芯片的正确摆放方向，即有倒角或斜面的一端面向胚胎，使靠近倒角或斜面的区间先跟胚胎接触，远离斜面的区间后跟胚胎接触。
所述区间区域包括多个依次排列的区间11在相邻的区间之间设置有分隔板，分隔板将区间区域分为相互独立的区间。所述区间用于封闭不同类型的水凝胶或溶液，在不同的使用环境中所需的区间的数量有所不同。例如，在冷冻应用中，如图1所示，生物芯片包括2个区间；在解冻应用中，如图3所示，生物芯片包括3个区间。此外，在一些实施例中，还可以设置4个区间，多出的一个区间可用于封闭不同浓度的溶液，以减慢渗透压的变化，使胚胎受到更小的渗透压伤害。
下面结合实际应用场景描述本申请实施例。
在对生物组织(如胚胎、卵子、细胞等生物材料)进行冷冻保存或解冻复苏过程中，根据本申请实施例的生物芯片用于封装在其中投放的化学品，该化学品可以为以水凝胶形式制成的固体形态或者为溶液形态。
在冷冻应用中使用水凝胶的情况下，生物芯片包括2个区间，这2个区间分别封装平衡液(ES)水凝胶和璃化冷冻液(VS)水凝胶。具体地，在生物芯片的底部贴上密封膜，生物芯片的区间就形成为2个凹槽。将ES水凝胶溶液和VS水凝胶溶液分别灌装到2个凹槽内，水凝胶固化后在生物芯片顶部贴上密封膜。此时各个区间的水凝胶就被区隔并密封起来，以增加保存时间。每个区间底部的两侧分别设置有固定槽13，固定槽13用于固定水凝 胶固化后的位置，避免在撕下密封膜时把水凝胶一并拉出区间。在生物芯片的一端设置有2个斜面，用于表示生物芯片的正确摆放方向，即靠近斜面的装有ES水凝胶的区间先与胚胎接触，远离斜面的装有VS水凝胶的区间后与胚胎接触。此外，密封膜上也可以印上方向标识(例如箭头)，指示生物芯片的正确摆放方向。进行冷冻时，在载体上加注培养液，将生物芯片的上下两片密封膜撕开，将生物芯片按照指示方向摆放好，使生物芯片紧贴在载体上方。接着使生物芯片相对载体移动，生物芯片区间内的ES水凝胶和VS水凝胶按顺序依次接触载体凹槽内的胚胎，投送冷冻保护剂。投送完成后，使生物芯片脱离载体，并把载体投入液氮里冷冻。
在解冻应用中使用水凝胶的情况下，生物芯片包括3个区间，这3个区间分别封装解冻液(TS)水凝胶、稀释液(DS)水凝胶和清洗液(WS)水凝胶。具体地，在生物芯片的底部贴上密封膜，生物芯片的区间就形成为3个凹槽。将TS、DS和WS水凝胶溶液分别灌装到3个凹槽内，水凝胶固化后在生物芯片顶部贴上密封膜。此时各个区间的水凝胶就被区隔并密封起来，以增加保存时间。每个区间底部的两侧分别设置有固定槽13，固定槽13用于固定水凝胶固化后的位置，避免在撕下密封膜时把水凝胶一并拉出区间。生物芯片的一端设置有2个斜面，用于表示生物芯片的正确摆放方向，即靠近斜面的装有TS水凝胶的区间先与胚胎接触，中间装有DS水凝胶的区间再与胚胎接触，远离斜面的装有WS水凝胶的区间最后与胚胎接触。此外，密封膜上也可以印上方向标识(例如箭头)，指示生物芯片的正确摆放方向。进行解冻时，将载体从液氮取出，并放到已经预热到37摄氏度的油相解冻液内进行解冻复温。待载体解冻复温后，将生物芯片的上下两片密封膜撕开，将生物芯片按照指示方向摆放好，使生物芯片紧贴在载体上方。接着使生物芯片相对载体移动，生物芯片区间内的TS、DS和WS水凝胶按顺序依次接触载体凹槽内的胚胎，移除胚胎内的冷冻保护剂。移除完成后，使生物芯片脱离载体，并使用玻璃毛细管从载体凹槽将胚胎回收到培养皿内进行培养。
在冷冻应用中使用溶液的情况下，生物芯片包括2个区间，分别封装ES溶液和VS溶液。具体地，在生物芯片的底部先贴上通透性薄膜，在生物芯片顶部贴上密封膜，这样生物芯片的区间就形成为2个凹槽。生物芯片的一 端设置有2个斜面，用于表示生物芯片的正确摆放方向，即靠近斜面的区间在使用的时候会加入ES溶液先跟胚胎接触，远离斜面的区间在使用的时候会加入VS溶液后跟胚胎接触。此外，密封膜上也可以印上方向表示(例如箭头)指示正确的摆放方向。进行冷冻时，在载体上加注培养液，将生物芯片顶部的密封膜撕开，将生物芯片按照指示方向摆放好，使生物芯片紧贴在载体上方。在相应的生物芯片区间加入需要的溶液(例如ES和VS)，然后使生物芯片相对载体移动，生物芯片区间内的ES和VS溶液会通过扩散作用穿越通透性薄膜，按顺序依次接触载体凹槽内的胚胎，投送冷冻保护剂。投送完成后，使生物芯片脱离载体，并把载体投入液氮里进行冷冻。
在解冻应用中使用溶液的情况下，生物芯片包括3个区间，这3个区间分别封装TS溶液、DS溶液和WS溶液。具体地，在生物芯片的底部先贴上通透性薄膜，在生物芯片顶部贴上密封膜，这样生物芯片的区间就形成为3个凹槽。生物芯片的一端设置有2个斜面，用于表示生物芯片的正确摆放方向，即靠近斜面的区间在使用时会加入TS溶液先与胚胎接触，中间的区间在使用时会加入DS溶液再与胚胎接触，远离斜面的区间在使用时会加入WS溶液最后与胚胎接触。此外，密封膜上也可以印上方向标识(例如箭头)，指示生物芯片的正确摆放方向。进行解冻时，将载体从液氮取出，并放到已经预热到37摄氏度的油相解冻液内进行解冻复温。待载体解冻复温后，将生物芯片顶部的密封膜撕开，将生物芯片按照指示方向摆放好，将生物芯片紧贴在载体上方。在相应的生物芯片区间加入需要的溶液(例如TS、DS和WS)，然后使生物芯片相对载体移动，生物芯片区间内的TS、DS和WS溶液会通过扩散作用穿越通透性薄膜，按顺序依次接触载体凹槽内的胚胎，让载体凹槽内和胚胎内的冷冻保护剂扩散到生物芯片的溶液里，达到移除胚胎内的冷冻保护剂的目的。移除完成后，使生物芯片脱离载体，并使用玻璃毛细管从载体凹槽将胚胎回收到培养皿内进行培养。
参考图4，当将溶液灌装到生物芯片的区间时，由于毛细作用，靠近两侧的溶液液面较高，形成一个弧面16，这个弧面会导致显微镜的光路产生变化，无法在弧面下清楚观察生物芯片下面的胚胎形态变化。由于水凝胶在固化前也是溶液状态，水凝胶的灌装也是面对同样的问题，溶液弧面在固化后 也保留下来，影响后面应用的光学效果，无法在弧面下清楚观察生物芯片下面的胚胎形态变化。为了解决该问题，参考图5至图7，在生物芯片的上方设置透明挡板18，所述透明挡板覆盖所述区间区域的中间部分。当生物芯片的底部贴上密封膜后，生物芯片的区间就形成在两侧有孔，中间中空的结构。
参考图8，使用水凝胶进行冷冻或解冻的情况下，水凝胶溶液可从透明挡板两侧的孔的其中一个孔灌装到区间内部，由于挡板的缘故，溶液会填满整个中空结构，而不会在挡板的下方形成弧面，弧面19只会出现在挡板两侧的孔的边缘，不影响在使用时使用显微镜观察载体凹槽内的胚胎的光路。水凝胶固化后，就可以在生物芯片顶部贴上密封膜。后续的使用操作步骤请参考以上描述，此处不再赘述。
继续参考图8，使用溶液进行冷冻或解冻的情况下，在使用生物芯片时，将生物芯片按照指示方向摆放好，使生物芯片紧贴在载体上方，然后从透明挡板两侧的孔加入需要的溶液到区间内部(例如在冷冻应用加入ES和VS，解冻应用加入TS、DS和WS)到合适的区间。由于挡板的缘故，溶液会填满整个中空结构，而不会在挡板的下方形成弧面，弧面19只会出现在挡板两侧的孔的边缘，不影响在使用时使用显微镜观察载体凹槽内的胚胎的光路。接下来使生物芯片和载体相对移动，后续的操作步骤请参考以上描述，此处不再赘述。
以上所述仅为本实用新型的实施例而已，并不用于限制本实用新型，对于本领域的技术人员来说，本实用新型可以有各种更改和变化。凡在本实用新型的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本实用新型的权利要求范围之内。

Claims (7)

  1. 一种用于生物组织冷冻保存或解冻复苏的生物芯片，其特征在于，所述生物芯片的四端为封闭结构；所述生物芯片包括：
    设置在中间位置的区间区域，所述区间区域包括多个依次排列的区间，所述区间之间设置有分隔板；每个区间的底部设置有固定槽；
    接合部，其设置在所述区间区域的两侧。
  2. 根据权利要求1所述的生物芯片，其特征在于，所述区间区域的底部还设置有密封膜，所述密封膜将所述多个区间形成为多个凹槽。
  3. 根据权利要求2所述的生物芯片，其特征在于，所述密封膜上具有方向标识。
  4. 根据权利要求1所述的生物芯片，其特征在于，所述区间区域的底部还设置有通透性薄膜，所述通透性薄膜将所述多个区间形成为多个凹槽。
  5. 根据权利要求1所述的生物芯片，其特征在于，所述生物芯片的一端具有一个或2个倒角或斜面。
  6. 根据权利要求1所述的生物芯片，其特征在于，所述生物芯片的上方还设置有透明挡板，所述透明挡板覆盖所述区间区域的中间部分。
  7. 根据权利要求1至6中任一项所述的生物芯片，其特征在于，所述区间区域设置有2个、3个或4个区间。
PCT/CN2022/078601 2021-03-03 2022-03-01 用于生物组织冷冻保存或解冻复苏的生物芯片 WO2022184050A1 (zh)

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