WO2022174775A1 - Use of antibody-drug conjugate targeting her2 in treatment of specific breast cancer - Google Patents

Use of antibody-drug conjugate targeting her2 in treatment of specific breast cancer Download PDF

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WO2022174775A1
WO2022174775A1 PCT/CN2022/076554 CN2022076554W WO2022174775A1 WO 2022174775 A1 WO2022174775 A1 WO 2022174775A1 CN 2022076554 W CN2022076554 W CN 2022076554W WO 2022174775 A1 WO2022174775 A1 WO 2022174775A1
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antibody
seq
breast cancer
use according
amino acid
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French (fr)
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Jianmin Fang
Xiaohong Su
Xuguang GUO
Ruyi HE
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Remegen Co Ltd
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Remegen Co Ltd
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Priority to JP2023549573A priority Critical patent/JP2024506708A/ja
Priority to EP22755548.9A priority patent/EP4294455A4/en
Priority to MX2023009376A priority patent/MX2023009376A/es
Priority to CA3206798A priority patent/CA3206798A1/en
Priority to CN202280015186.0A priority patent/CN117460538A/zh
Priority to AU2022222937A priority patent/AU2022222937A1/en
Application filed by Remegen Co Ltd filed Critical Remegen Co Ltd
Priority to IL305095A priority patent/IL305095A/en
Priority to KR1020237027221A priority patent/KR20230146526A/ko
Publication of WO2022174775A1 publication Critical patent/WO2022174775A1/en
Priority to US18/450,966 priority patent/US20240148894A1/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/68031—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being an auristatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6851—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell
    • A61K47/6855—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell the tumour determinant being from breast cancer cell
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6889—Conjugates wherein the antibody being the modifying agent and wherein the linker, binder or spacer confers particular properties to the conjugates, e.g. peptidic enzyme-labile linkers or acid-labile linkers, providing for an acid-labile immuno conjugate wherein the drug may be released from its antibody conjugated part in an acidic, e.g. tumoural or environment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes

Definitions

  • the present disclosure relates to the field of precise treatment of cancers, to use of an antibody-drug conjugate targeting HER2 (Human epidermal growth factor receptor 2) in the treatment of breast cancers with different biological behaviors, clinical indicators, and disease molecular types.
  • HER2 Human epidermal growth factor receptor 2
  • Precision Medicine is also often understood as Personalized Medicine, in which "precision” in a broad sense refers to the most appropriate treatment for the right patient, i.e., it is necessary to determined clinically which therapeutic drugs are the most effective for a certain population, and which therapeutic drugs may be ineffective or bring greater toxic and side effects; "Personalized Information" is needed because each patient is unique, and then it is required clinically to make the correct classification of the specific diseases of specific patients and then give the correct and optimal therapeutic drugs, thereby providing more effective, safer and more economical medical services to the patients.
  • Breast cancer is a common malignant tumor in women. Due to changes in people's lifestyle concepts and ecological environment, the incidence of breast cancer is also increasing significantly. In the past 100 years, the treatment of breast cancer went through the proposal and promotion of breast conserving surgery, adjuvant therapy, endocrine therapy, targeted therapy, etc. The related clinical practice and clinical research results have accumulated a lot of experience and data for the treatment of the disease and improved the overall level of the diagnosis of the disease. However, the clinical treatment of breast cancer has always been based on histopathology, but when using the same regimen in breast cancer patients of the same pathological type and at the same clinical stage, the sensitivity of treatment and prognosis of patients vary greatly. Obviously, the traditional histopathological diagnosis and clinical staging can no longer well meet the development needs of tumor research.
  • breast cancer patients have different biological behaviors and clinical indicators, such as age, ethnicity, economic history, tumor family history, pathological type, pathological grade, tumor location, tumor size, lymph node metastasis, clinical stage and even disease molecular types. This is also the main reason for the obvious differences in treatment response, survival, and other aspects of breast cancers of the same histological type. Therefore, in clinical practice, the treatment should be classified according to patients'different biological behaviors, clinical indicators, and disease molecular types, etc., in order to achieve the purpose of precise personalized treatment (i.e., to give optimal therapeutic drugs according to individual differences) .
  • Chinese patent publication no. CN105008398A discloses an antibody-drug conjugate (i.e., Disitamab vedotin) that can specifically bind to HER2 and has a drug moiety of MMAE.
  • an antibody-drug conjugate i.e., Disitamab vedotin
  • HER2-expressing cancer indications including breast cancer, such as gastric and urothelial cancers, and HER2-low expressing (IHC 2+/FISH-or IHC 1+) cancer indications, such as HER2-low expressing breast cancer.
  • the new drug has also been granted Fast Track designation by the U.S. FDA for the treatment of urothelial carcinoma and gastric cancer.
  • the present disclosure provides methods and uses for treating breast cancer patients with an anti-HER2 antibody-drug conjugate (ADC) .
  • ADC anti-HER2 antibody-drug conjugate
  • the progression-free survival time in the Disitamab vedotin treatment group was 12.5 months, and the progression-free survival time in the capecitabine+lapatinib group was 5.6 months.
  • the progression-free survival time in the Disitamab vedotin treatment group was 10.9 months and the progression-free survival time in the capecitabine+lapatinib group was 5.6 months.
  • the efficacy of the antibody-drug conjugates (ADC, especially Disitamab vedotin) provided by the present invention did not show a statistically significant advantage in overall sample of breast cancer patients and in a subgroup of breast cancer patients without bone metastasis or in a subgroup of breast cancer patients without viscera metastasis, and thus the superior results seen in breast cancer patients with liver metastasis or breast cancer patients without lung metastasis were surprising.
  • Disitamab vedotin effectively prolonged the disease progression-free time and survival time of breast cancer patients with liver metastasis or breast cancer patients without lung metastasis, thereby providing patients with more precise treatment options. That is to say, the application of the antibody-drug conjugates (ADC, especially Disitamab vedotin) provided by the present invention in the treatment of breast cancer patients with liver metastasis or the treatment of breast cancer patients without lung metastasis can achieve "precise treatment" for the corresponding patients.
  • ADC antibody-drug conjugates
  • Disitamab vedotin the clinical application of Disitamab vedotin treatment group in breast cancer patients with liver metastasis and in breast cancer patients without lung metastasis has great significance and greatly prolongs the disease progression and possible survival time of the patients.
  • an antibody-drug conjugate in the preparation of a medicine for treating a breast cancer patient with liver metastasis, wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents anti-Her2 (Human epidermal growth factor receptor 2) antibody; L represents a linker; U represents a conjugated cytotoxic molecule; and n is an integer from 1 to 8, and represents the number of cytotoxic molecules bound to each antibody, and wherein: the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin; the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of sulfhydryl conjugation,
  • ADC antibody-drug conjugate
  • an antibody-drug conjugate in the preparation of a medicine for treating of a breast cancer patient without lung metastasis, wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents anti-Her2 (Human epidermal growth factor receptor 2) antibody; L represents a linker; U represents a conjugated cytotoxic molecule; and n is an integer from 1 to 8, and represents the number of cytotoxic molecules bound to each antibody, and wherein: the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin; the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of sulfhydryl conjugation
  • the breast cancer patient is positive for HER2 expression.
  • a sample obtained from the breast cancer of the patient is HER2 positive.
  • the sample obtained from the breast cancer of the patient is HER2 positive based on a fluorescence in situ hybridization (FISH) assay (FISH) and/or immunohistochemistry (IHC) assay.
  • FISH fluorescence in situ hybridization
  • IHC immunohistochemistry
  • HER2 expression in the sample obtained from the breast cancer of the patient is: IHC3+; IHC2+or IHC3+; IHC2+or FISH+; IHC3+or FISH+; IHC2+and FISH+; IHC3+and FISH+; or IHC3+and FISH-or not detected.
  • a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive and/or progesterone receptor (PR) positive.
  • a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive or progesterone receptor (PR) positive.
  • a sample obtained from the breast cancer of the patient is ER negative and PR negative.
  • the patient has locally advanced or metastatic breast cancer.
  • the patient has stage IV breast cancer.
  • the patient has unresectable breast cancer.
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ ID NO: 4) , and an HCDR3 comprising the amino acid sequence of ARNYLFDH (SEQ ID NO: 5) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of QDVGTA (SEQ ID NO: 6) , a LCDR2 comprising the amino acid sequence of WAS (SEQ ID NO: 7) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the amino acid sequence of RVNPDHGDSYYNQKFKD (SEQ ID NO: 12) , and an HCDR3 comprising the amino acid sequence of ARNYLFDHW (SEQ ID NO: 13) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of KASQDVGTAVA (SEQ ID NO: 14) , a LCDR2 comprising the amino acid sequence of WASIRHT (SEQ ID NO: 15) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and/or wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSY
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYN
  • the antibody is a murine, chimeric, or humanized antibody. In some embodiments, the antibody is a human IgG antibody. In some embodiments, the antibody is a human IgG1, IgG2, or IgG4 antibody.
  • the amino acid sequence of the heavy chain of the antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain of the antibody is shown in SEQ ID NO: 2. In some embodiments, the amino acid sequence of the heavy chain of the antibody is shown in SEQ ID NO: 1 without the C-terminal lysine, and the amino acid sequence of the light chain of the antibody is shown in SEQ ID NO: 2.
  • the antibody-drug conjugate is Disitamab vedotin or a biosimilar thereof.
  • the average DAR (i.e., Drug-to-Antibody Ratio) value of the antibody-drug conjugate is any number from 2 to 7. In some embodiments, the average DAR value is 4 ⁇ 0.5.
  • the patient has previously received one or more prior treatments of chemotherapy drugs, targeted therapy, immunotherapy, and endocrine therapy.
  • the patient has previously received taxane systemic therapy.
  • the patient has previously received systemic therapy with trastuzumab or a biosimilar thereof at least once.
  • the medicine is to be administered intranasally, subcutaneously, intradermally, intramuscularly or intravenously.
  • the medicine is to be administered at a dose of 2.0 mg/kg every 2 weeks.
  • the medicine is to be administered as a monotherapy.
  • administration of the antibody-drug conjugate to the breast cancer patient results in improved progression-free survival (PFS) , as compared to administration of capecitabine and lapatinib.
  • PFS progression-free survival
  • an antibody-drug conjugate comprising administering to a patient in need thereof a therapeutically effective amount of an antibody-drug conjugate (ADC) , wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents an antibody that specifically binds human epidermal growth factor receptor 2 (HER2) ; L represents a linker; U represents a cytotoxic molecule; and n is an integer from 1 to 8 representing a number of cytotoxic molecule (s) conjugated to each antibody, and wherein: the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin; the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of
  • a sample obtained from the breast cancer of the patient is HER2 positive.
  • the breast cancer expresses HER2, e.g., overexpresses HER2.
  • a sample obtained from the breast cancer of the patient is HER2 positive based on a fluorescence in situ hybridization (FISH) assay (FISH) and/or immunohistochemistry (IHC) assay.
  • FISH fluorescence in situ hybridization
  • IHC immunohistochemistry
  • HER2 expression in the sample obtained from the breast cancer of the patient is: IHC3+; IHC2+or IHC3+; IHC2+or FISH+; IHC3+or FISH+; IHC2+and FISH+; IHC3+and FISH+; or IHC3+and FISH-or not detected.
  • a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive and/or progesterone receptor (PR) positive.
  • a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive and progesterone receptor (PR) positive.
  • a sample obtained from the breast cancer of the patient is ER negative and PR negative.
  • the patient has locally advanced or metastatic breast cancer. In some embodiments, the patient has stage IV breast cancer. In some embodiments, the patient has unresectable breast cancer. In some embodiments, the breast cancer is infiltrating locally advanced or metastatic breast cancer as established by histology and/or cytology and is unresectable.
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ ID NO: 4) , and an HCDR3 comprising the amino acid sequence of ARNYLFDH (SEQ ID NO: 5) ; and/or wherein the VL region comprises a LCDR1 comprising the amino acid sequence of QDVGTA (SEQ ID NO: 6) , a LCDR2 comprising the amino acid sequence of WAS (SEQ ID NO: 7) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ ID NO: 4) , and an HCDR3 comprising the amino acid sequence of ARNYLFDH (SEQ ID NO: 5) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of QDVGTA (SEQ ID NO: 6) , a LCDR2 comprising the amino acid sequence of WAS (SEQ ID NO: 7) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the amino acid sequence of RVNPDHGDSYYNQKFKD (SEQ ID NO: 12) , and an HCDR3 comprising the amino acid sequence of ARNYLFDHW (SEQ ID NO: 13) ; and/or wherein the VL region comprises a LCDR1 comprising the amino acid sequence of KASQDVGTAVA (SEQ ID NO: 14) , a LCDR2 comprising the amino acid sequence of WASIRHT (SEQ ID NO: 15) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 compris
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the amino acid sequence of RVNPDHGDSYYNQKFKD (SEQ ID NO: 12) , and an HCDR3 comprising the amino acid sequence of ARNYLFDHW (SEQ ID NO: 13) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of KASQDVGTAVA (SEQ ID NO: 14) , a LCDR2 comprising the amino acid sequence of WASIRHT (SEQ ID NO: 15) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and/or wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSY
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYN
  • the antibody is a human IgG antibody, e.g., a human IgG1, IgG2, or IgG4 antibody.
  • the antibody comprises a human Fc region, e.g., a human IgG1, IgG2, or IgG4 Fc region.
  • the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 1 and a light chain comprising the amino acid sequence of SEQ ID NO: 2.
  • the antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 1 without the C-terminal lysine and a light chain comprising the amino acid sequence of SEQ ID NO: 2.
  • the antibody-drug conjugate is Disitamab vedotin or a biosimilar thereof.
  • the average Drug-to-Antibody Ratio (DAR) of the antibody-drug conjugate is any number from 2 to 7, e.g., the average DAR value is 4 ⁇ 0.5.
  • the patient prior to administration of the ADC, the patient has previously received one or more prior treatments comprising a chemotherapeutic agent, targeted therapy, immunotherapy, or endocrine therapy. In some embodiments, prior to administration of the ADC, the patient has received a taxane systemic therapy. In some embodiments, prior to administration of the ADC, the patient has received systemic therapy with trastuzumab or a biosimilar thereof at least once. In some embodiments, prior to administration of the ADC, the patient has received systemic therapy with an anti-HER2 antibody at least once. In some embodiments, the ADC is administered intranasally, subcutaneously, intradermally, intramuscularly, or intravenously.
  • the ADC is administered at a dose of 2.0 mg/kg every 2 weeks. In some embodiments, the ADC is administered as a monotherapy. In some embodiments, administration of the ADC results in improved progression-free survival (PFS) of the patient, as compared to administration of capecitabine and lapatinib. In some embodiments, the ADC is administered in a pharmaceutical composition comprising the ADC and a pharmaceutically acceptable carrier.
  • FIG. 1 is a schematic diagram of the structure of monomethyl auristatin E (MMAE) .
  • MMAE monomethyl auristatin E
  • FIG. 2 is a schematic diagram of exemplary structures of the antibody-drug conjugates of the structural general formula Ab- (L-U) n of the present disclosure under one of the possible conjugation conditions (L is linked to one or more interchain disulfide bond sites of the antibody through sulfhydryl conjugation) where n is 1, 2, 3, 4, 5, 6, 7, and 8, respectively; L is Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) ; U is MMAE; and the structure of "-L-U" is as follows:
  • the determination or numbering method of the complementary determining regions (CDRs) of the variable domains of antibodies includes IMGT, Kabat, Chothia, AbM, and Contact methods which are well known in the art.
  • the "antibody” used in the present invention encompasses a variety of antibody structures including, but not limited to, monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies) , and antigen binding fragments.
  • Antigen binding fragment used in the present invention refers to an antibody fragment comprising a heavy chain variable region or a light chain variable region of the antibody and being sufficient to retain the same binding specificity as its source antibody and sufficient affinity.
  • the antigen binding fragments comprise Fab, F (ab') , and F (ab') 2, which contain at least one immunoglobulin fragment sufficient to make a specific antigen bind to the polypeptide.
  • the above fragments can be prepared by synthesis, or by an enzymic method, or by chemical cutting of intact immunoglobulin, or genetically engineered by using recombinant DNA techniques. The production methods of the above fragments are well known in the art.
  • murine antibody as used in the present invention is a monoclonal antibody prepared according to the knowledge and skill in the art. During preparation, acorresponding antigen was injected into the test subjects, and then hybridomas expressing an antibody having the desired sequence or functional characteristics were isolated.
  • the murine antibody or antigen binding fragment thereof can further comprise a light chain constant region of murine ⁇ or ⁇ chain or a variant thereof, or further comprises a heavy chain constant region of murine IgG1, IgG2, IgG3, or a variant thereof.
  • chimeric antibody as used in the present invention is an antibody that is a fusion of a variable region of a murine antibody with a constant region of a human antibody and can reduce immune response induced by the murine antibody.
  • hybridomas which secrete a murine specific monoclonal antibody are first established.
  • variable region genes are cloned from murine hybridoma cells, and as required, constant region genes are cloned from the human antibody.
  • the mouse variable region genes and the human constant region genes are linked to form a chimeric gene and inserted into a human vector.
  • chimeric antibody molecules are expressed in a eukaryotic industrial system or a prokaryotic industrial system.
  • the antibody light chain of the chimeric antibody further comprises a light chain constant region of human ⁇ or ⁇ chain or a variant thereof.
  • the antibody heavy chain of the chimeric antibody further comprises a heavy chain constant region of human IgG1, IgG2, IgG3, IgG4, or a variant thereof.
  • the constant region of the human antibody can be selected from the heavy chain constant region of human IgG1, IgG2, IgG3, or IgG4, or a variant thereof, and preferably comprise the heavy chain constant region of human IgG2 or IgG4.
  • IgG4 which has no ADCC toxicity (antibody-dependent cell-mediated cytotoxicity) after an amino acid mutation occurred is used.
  • humanized antibody refers to an antibody generated by grafting of a mouse CDR sequence into human antibody variable region framework (i.e., human germline antibody framework sequences of different types) . It comprises a CDR region derived from a non-human antibody and the rest of the antibody molecule is derived from one human antibody (or several human antibodies) .
  • FR segments can be modified (Jones et al., Nature, 321: 522-525, 1986; Verhoeyen et al., Science, 239: 1534-1536, 1988; and Riechmann et al., Nature, 332: 323-327, 1988) .
  • the humanized antibodies or fragments thereof according to the invention can be prepared by techniques known to those skilled in the art (e.g., as described in Singer et al., J. Immun. 150: 2844-2857, 1992; Mountain et al., Biotechnol. Genet. Eng. Rev., 10: 1-142, 1992; or Bebbington et al., Bio/Technology, 10: 169-175, 1992) .
  • average “DAR” value as used in the present invention refers to the average value of the number of drugs linked to the antibody in the antibody-drug conjugate preparation.
  • sulfhydryl conjugation refers to a conjugation means by which the linker is covalently linked to a free sulfhydryl group on the antibody.
  • Cysteine exists in the form of a disulfide bond in the antibody, and there are 4 pairs of interchain disulfide bonds in an IgG antibody, which are easy to be reduced. Therefore, during the preparation of the antibody-drug conjugate, the 4 pairs of interchain disulfide bonds in the IgG antibody are often reduced, which produces the above-mentioned "free sulfhydryl group on the antibody” .
  • an IgG antibody will have a maximum of 8 sulfhydryl conjugation sites.
  • the antibody-drug conjugate involved has the structure of the general formula Ab- (L-U) n, where Ab represents anti-HER2 (Human epidermal growth factor receptor 2) antibody; L represents a linker; U represents conjugated cytotoxic molecules; and n is an integer from 1 to 8 (such as 1, 2, 3, 4, 5, 6, 7, or 8) , and represents the number of cytotoxic molecules bound to each antibody.
  • the cytotoxic molecule is an auristatin, or an analog or derivative thereof.
  • Auristatins are derivatives of the natural product dolastatin.
  • Exemplary auristatins include dolostatin-10, auristatin E, auristatin T, MMAE (N-methylvaline-valine-dolaisoleuine-dolaproine-norephedrine or monomethyl auristatin E) and MMAF (N-methylvaline-valine-dolaisoleuine-dolaproine-phenylalanine or dovaline-valine-dolaisoleunine-dolaproine-phenylalanine) , AEB (ester produced by reacting auristatin E with paraacetyl benzoic acid) , AEVB (ester produced by reacting auristatin E with benzoylvaleric acid) , and AFP (dimethylvaline-valine-dolaisoleuine
  • the cytotoxic molecule is MMAE. In other embodiments, the cytotoxic agent is MMAF.
  • the anti-HER2 (Human epidermal growth factor receptor 2) antibody or the functional fragment thereof in the antibody-drug conjugate provided by the present invention comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin;
  • the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) ;
  • the cytotoxic molecules U comprise MMAE (monomethyl auristatin E) .
  • the linker L is covalently linked to the antibody by means of sulfhydryl conjugation, and the linking site is the interchain disulfide bond site of the antibody.
  • the antibody-drug conjugates of the present invention is a mixture of antibody-drug conjugates linked with 2-7 cytotoxic molecules, where the average DAR(i.e., Drug-to-Antibody Ratio) value of the antibody-drug conjugates is any number from 2 to 7; more preferably, the average DAR value of the antibody-drug conjugates of the present invention is approximately equal to 2, 3, 4, 5, 6, or 7. In some specific examples of the present invention, the average DAR value of the antibody-drug conjugates of the present invention is 4 ⁇ 0.5.
  • the corresponding CDRs 1-3 of the heavy chain variable regions and the light chain variable region of the anti-HER2 antibody involved in the present invention are as follows (IMGT numbering) :
  • HCDR1 GYTFTDYY SEQ ID NO: 3
  • HCDR2 VNPDHGDS SEQ ID NO: 4
  • HCDR3 ARNYLFDH SEQ ID NO: 5
  • LCDR1 QDVGTA
  • LCDR2 WAS
  • SEQ ID NO: 7 LCDR3: HQFATYT SEQ ID NO: 8
  • the corresponding CDRs 1-3 of the heavy chain variable regions and the light chain variable region of the anti-HER2 antibody involved in the present invention are as follows:
  • HCDR1 DYYIH SEQ ID NO: 11
  • HCDR2 RVNPDHGDSYYNQKFKD
  • HCDR3 ARNYLFDHW
  • LCDR1 KASQDVGTAVA
  • LCDR2 WASIRHT SEQ ID NO: 15
  • LCDR3 HQFATYT SEQ ID NO: 8
  • the anti-HER2 antibody comprises the corresponding CDRs 1-3 of the heavy chain variable regions and the light chain variable region represented by SEQ ID Nos: 3-8, but with 1, 2, or 3 substitutions (e.g., conservative substitutions) , insertions, or deletions relative to SEQ ID Nos: 3-8, but an anti-HER2 antibody comprising that sequence retains the ability to bind to HER2.
  • the anti-HER2 antibody comprises the corresponding CDRs 1-3 of the heavy chain variable regions and the light chain variable region represented by SEQ ID Nos: 11-15 and 8, but with 1, 2, or 3 substitutions (e.g., conservative substitutions) , insertions, or deletions relative to SEQ ID Nos: 11-15 and 8, but an anti-HER2 antibody comprising that sequence retains the ability to bind to HER2.
  • the anti-HER2 (Human epidermal growth factor receptor 2) antibody in the antibody-drug conjugate provided by the present invention is murine, chimeric, humanized or fully human, preferably a humanized monoclonal antibody. In some embodiments, the antibody is a monoclonal antibody.
  • the anti-HER2 (Human epidermal growth factor receptor 2) antibody in the antibody-drug conjugate provided by the present invention is IgG, including IgG1, IgG2, IgG3, and IgG4, and more preferably IgG1, IgG2, and IgG4.
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%identity to the sequence EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and/or wherein the VL region comprises an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%identity to the sequence DIQMTQ
  • the VH sequence (e.g., having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%identity to SEQ ID NO: 9) contains substitutions (e.g., conservative substitutions) , insertions, or deletions relative to SEQ ID NO: 9, but an anti-HER2 antibody comprising that sequence retains the ability to bind to HER2.
  • substitutions, insertions, or deletions occur in regions outside the CDRs (i.e., in the FRs) .
  • the VL sequence (e.g., having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%identity to SEQ ID NO: 10) contains substitutions (e.g., conservative substitutions) , insertions, or deletions relative to SEQ ID NO: 10, but an anti-HER2 antibody comprising that sequence retains the ability to bind to HER2.
  • substitutions e.g., conservative substitutions
  • insertions, or deletions relative to SEQ ID NO: 10
  • an anti-HER2 antibody comprising that sequence retains the ability to bind to HER2.
  • a total of 1 to 10 amino acids have been substituted, inserted and/or deleted in SEQ ID NO: 10.
  • substitutions, insertions, or deletions occur in regions outside the CDRs (i.e., in the FRs) .
  • the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYN
  • the antibody-drug conjugate of the present invention is Disitamab vedotin, which is an antibody-drug conjugate targeting a HER2 target, where the linker moiety L is Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) ; the cytotoxic molecules U comprise MMAE (monomethyl auristatin E) ; the linker L is covalently linked to the antibody by means of sulfhydryl conjugation; and the average DAR value is 4 ⁇ 0.5.
  • the linker moiety L is Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB)
  • the cytotoxic molecules U comprise MMAE (monomethyl auristatin E)
  • the linker L is covalently linked to the antibody by means of sulfhydryl conjugation
  • the average DAR value is 4 ⁇ 0.5.
  • the heavy chain amino acid sequence of the antibody Ab in the antibody-drug conjugate involved in the present invention is shown in SEQ ID NO: 1, and the light chain amino acid sequence thereof is shown in SEQ ID NO: 2.
  • the heavy chain comprises the amino acid sequence of SEQ ID NO: 1 without the C-terminal lysine.
  • breast cancer involved in the present invention is HER2 expression-positive breast cancer, preferably infiltrating locally advanced or metastatic breast cancer as established by histology and/or cytology and is unresectable.
  • the patient is a stage IV breast cancer patient.
  • the patient is less than 65 years old. In some embodiments, the patient is equal to or greater than 65 years old.
  • a sample from the breast cancer of the patient is HER2 positive, e.g., HER2 positive based on a fluorescence in situ hybridization (FISH) assay (FISH+) and/or immunohistochemistry (IHC) .
  • a sample from the breast cancer of the patient is IHC2+or IHC3+.
  • a sample from the breast cancer of the patient is IHC2+or FISH+.
  • a sample from the breast cancer of the patient is IHC3+or FISH+.
  • a sample from the breast cancer of the patient is IHC2+and FISH+.
  • a sample from the breast cancer of the patient is IHC3+and FISH+.
  • a sample from the breast cancer of the patient is IHC3+and FISH-or not detected.
  • a sample from the breast cancer of the patient is estrogen receptor (ER) positive. In some embodiments, a sample from the breast cancer of the patient is estrogen receptor (ER) negative. In some embodiments, a sample from the breast cancer of the patient is progesterone receptor (PR) positive. In some embodiments, a sample from the breast cancer of the patient is progesterone receptor (PR) negative. In some embodiments, a sample from the breast cancer of the patient is ER+and/or PR+. In some embodiments, asample from the breast cancer of the patient is ER-and PR-.
  • patients for treatment according to the present invention have previously received one or more prior treatments of chemotherapy drugs, targeted therapy, immunotherapy, and endocrine therapy; preferably, they have previously received taxane systemic therapy; or they must have previously received systemic therapy with trastuzumab or a biosimilar thereof at least once.
  • the antibody-drug conjugate or medicine of the present invention may be administered intranasally, subcutaneously, intradermally, intramuscularly or intravenously. It is administered at a dose of 2.0 mg/kg every 2 weeks.
  • the medicine comprises the antibody-drug conjugate and a pharmaceutically acceptable carrier.
  • administration of the antibody-drug conjugate to the breast cancer patient results in improved progression-free survival (PFS) , as compared to administration of capecitabine and lapatinib.
  • the antibody-drug conjugate is administered as a monotherapy.
  • Embodiment 1 Use of an antibody-drug conjugate (ADC) in the preparation of a medicine for treating a breast cancer patient with liver metastasis, wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents anti-Her2 (Human epidermal growth factor receptor 2) antibody; L represents a linker; U represents a conjugated cytotoxic molecule; and n is an integer from 1 to 8, and represents the number of cytotoxic molecules bound to each antibody, and wherein:
  • the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin;
  • the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of sulfhydryl conjugation, and the linking site is the interchain disulfide bond site of the antibody; and
  • the cytotoxic molecule U comprises MMAE (monomethyl auristatin E) .
  • Embodiment 2 Use of an antibody-drug conjugate (ADC) in the preparation of a medicine for treating of a breast cancer patient without lung metastasis, wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents anti-Her2 (Human epidermal growth factor receptor 2) antibody; L represents a linker; U represents a conjugated cytotoxic molecule; and n is an integer from 1 to 8, and represents the number of cytotoxic molecules bound to each antibody, and wherein:
  • the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin;
  • the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of sulfhydryl conjugation, and the linking site is the interchain disulfide bond site of the antibody; and
  • the cytotoxic molecule U comprises MMAE (monomethyl auristatin E) .
  • Embodiment 3 The use according to embodiment 1 or 2, wherein the breast cancer patient is positive for HER2 expression.
  • Embodiment 4 The use according to embodiment 3, wherein a sample obtained from the breast cancer of the patient is HER2 positive.
  • Embodiment 5 The use according to embodiment 4, wherein the sample obtained from the breast cancer of the patient is HER2 positive based on a fluorescence in situ hybridization (FISH) assay (FISH) and/or immunohistochemistry (IHC) assay.
  • FISH fluorescence in situ hybridization
  • IHC immunohistochemistry
  • Embodiment 6 The use according to embodiment 5, wherein HER2 expression in the sample obtained from the breast cancer of the patient is: IHC3+; IHC2+or IHC3+; IHC2+or FISH+; IHC3+or FISH+; IHC2+and FISH+; IHC3+and FISH+; or IHC3+and FISH-or not detected.
  • Embodiment 7 The use according to any one of embodiments 1-6, wherein a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive and/or progesterone receptor (PR) positive; or wherein a sample obtained from the breast cancer of the patient is ER negative and PR negative.
  • ER estrogen receptor
  • PR progesterone receptor
  • Embodiment 8 The use according to any one of embodiments 1-7, wherein the patient has locally advanced or metastatic breast cancer.
  • Embodiment 9 The use according to any one of embodiments 1-7, wherein the patient has stage IV breast cancer.
  • Embodiment 10 The use according to any one of embodiments 1-9, wherein the patient has unresectable breast cancer.
  • Embodiment 11 The use according to embodiment 3, wherein the breast cancer is infiltrating locally advanced or metastatic breast cancer as established by histology and/or cytology and is unresectable.
  • Embodiment 12 The use according to any one of embodiments 1-11, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ ID NO: 4) , and an HCDR3 comprising the amino acid sequence of ARNYLFDH (SEQ ID NO: 5) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of QDVGTA (SEQ ID NO: 6) , a LCDR2 comprising the amino acid sequence of WAS (SEQ ID NO: 7) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an
  • Embodiment 13 The use according to any one of embodiments 1-11, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the amino acid sequence of RVNPDHGDSYYNQKFKD (SEQ ID NO: 12) , and an HCDR3 comprising the amino acid sequence of ARNYLFDHW (SEQ ID NO: 13) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of KASQDVGTAVA (SEQ ID NO: 14) , a LCDR2 comprising the amino acid sequence of WASIRHT (SEQ ID NO: 15) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH heavy chain variable
  • VL light chain variable
  • Embodiment 14 The use according to any one of embodiments 1-13, wherein the antibody is a murine, chimeric, or humanized antibody.
  • Embodiment 15 The use according to any one of embodiments 1-13, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHT GVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDY
  • Embodiment 16 The use according to embodiment 14 or embodiment 15, wherein the antibody is a human IgG antibody.
  • Embodiment 17 The use according to embodiment 16, wherein the antibody is a human IgG1, IgG2, or IgG4 antibody.
  • Embodiment 18 The use according to any one of embodiments 1-11, wherein the amino acid sequence of the heavy chain of the antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain of the antibody is shown in SEQ ID NO: 2.
  • Embodiment 19 The use according to any one of embodiments 1-11, wherein the antibody-drug conjugate is Disitamab vedotin or a biosimilar thereof.
  • Embodiment 20 The use according to any one of embodiments 1-19, wherein the average DAR (i.e., Drug-to-Antibody Ratio) value of the antibody-drug conjugate is any number from 2 to 7.
  • DAR i.e., Drug-to-Antibody Ratio
  • Embodiment 21 The use according to embodiment 20, wherein the average DAR value is 4 ⁇ 0.5.
  • Embodiment 22 The use according to any one of embodiments 1-21, wherein the patient has previously received one or more prior treatments of chemotherapy drugs, targeted therapy, immunotherapy, and endocrine therapy.
  • Embodiment 23 The use according to embodiment 22, wherein the patient has previously received taxane systemic therapy.
  • Embodiment 24 The use according to embodiment 22, wherein the patient has previously received systemic therapy with trastuzumab or a biosimilar thereof at least once.
  • Embodiment 25 The use according to any one of embodiments 1-24, wherein the medicine is administered intranasally, subcutaneously, intradermally, intramuscularly or intravenously.
  • Embodiment 26 The use according to any one of embodiments 1-25, wherein the antibody-drug conjugate is administered at a dose of 2.0 mg/kg every 2 weeks.
  • Embodiment 27 The use according to any one of embodiments 1-26, wherein the antibody-drug conjugate is administered as a monotherapy.
  • Embodiment 28 The use according to any one of embodiments 1-27, wherein administration of the antibody-drug conjugate to the breast cancer patient results in improved progression-free survival (PFS) , as compared to administration of capecitabine and lapatinib.
  • PFS progression-free survival
  • Embodiment 29 A method of treating breast cancer, comprising administering to a patient in need thereof a therapeutically effective amount of an antibody-drug conjugate (ADC) , wherein the antibody-drug conjugate has the structure of the general formula Ab- (L-U) n , wherein Ab represents an antibody that specifically binds human epidermal growth factor receptor 2 (HER2) ; L represents a linker; U represents a cytotoxic molecule; and n is an integer from 1 to 8 representing a number of cytotoxic molecule (s) conjugated to each antibody, and wherein:
  • ADC antibody-drug conjugate
  • the antibody comprises a heavy chain variable region and a light chain variable region, where the CDR of the heavy chain variable region and/or the CDR of the light chain variable region have the same CDR sequences as Disitamab vedotin;
  • the linker L comprises Maleimido-Caproyl-Valine-Citrulline-p-Aminobenzyloxy (mc-vc-pAB) and is covalently linked to the antibody by means of sulfhydryl conjugation, and the linking site is the interchain disulfide bond site of the antibody;
  • the cytotoxic molecules U comprise MMAE (monomethyl auristatin E) ;
  • the patient has a liver metastasis and/or does not have a lung metastasis.
  • Embodiment 30 The method of embodiment 29, wherein the breast cancer patient is positive for HER2 expression.
  • Embodiment 31 The method of embodiment 30, wherein a sample obtained from the breast cancer of the patient is HER2 positive.
  • Embodiment 32 The method of embodiment 31, wherein the sample obtained from the breast cancer of the patient is HER2 positive based on a fluorescence in situ hybridization (FISH) assay (FISH) and/or immunohistochemistry (IHC) assay.
  • FISH fluorescence in situ hybridization
  • IHC immunohistochemistry
  • Embodiment 33 The method of embodiment 32, wherein HER2 expression in the sample obtained from the breast cancer of the patient is: IHC3+; IHC2+or IHC3+; IHC2+or FISH+; IHC3+or FISH+; IHC2+and FISH+; IHC3+and FISH+; or IHC3+and FISH-or not detected.
  • Embodiment 34 The method of any one of embodiments 29-33, wherein a sample obtained from the breast cancer of the patient is estrogen receptor (ER) positive and/or progesterone receptor (PR) positive; or wherein a sample obtained from the breast cancer of the patient is ER negative and PR negative.
  • ER estrogen receptor
  • PR progesterone receptor
  • Embodiment 35 The method of any one of embodiments 29-34, wherein the patient has locally advanced or metastatic breast cancer.
  • Embodiment 36 The method of any one of embodiments 29-34, wherein the patient has stage IV breast cancer.
  • Embodiment 37 The method of any one of embodiments 29-36, wherein the patient has unresectable breast cancer.
  • Embodiment 38 The method of any one of embodiments 29-36, wherein the breast cancer is infiltrating locally advanced or metastatic breast cancer as established by histology and/or cytology and is unresectable.
  • Embodiment 39 The method of any one of embodiments 29-38, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an HCDR2 comprising the amino acid sequence of VNPDHGDS (SEQ ID NO: 4) , and an HCDR3 comprising the amino acid sequence of ARNYLFDH (SEQ ID NO: 5) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of QDVGTA (SEQ ID NO: 6) , a LCDR2 comprising the amino acid sequence of WAS (SEQ ID NO: 7) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of GYTFTDYY (SEQ ID NO: 3) , an
  • Embodiment 40 The method of any one of embodiments 29-38, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises an HCDR1 comprising the amino acid sequence of DYYIH (SEQ ID NO: 11) , an HCDR2 comprising the amino acid sequence of RVNPDHGDSYYNQKFKD (SEQ ID NO: 12) , and an HCDR3 comprising the amino acid sequence of ARNYLFDHW (SEQ ID NO: 13) ; and wherein the VL region comprises a LCDR1 comprising the amino acid sequence of KASQDVGTAVA (SEQ ID NO: 14) , a LCDR2 comprising the amino acid sequence of WASIRHT (SEQ ID NO: 15) , and a LCDR3 comprising the amino acid sequence of HQFATYT (SEQ ID NO: 8) .
  • VH region comprises an HCDR1 comprising the amino acid sequence of DYYI
  • Embodiment 41 The method of any one of embodiments 29-40, wherein the antibody is a murine, chimeric, or humanized antibody.
  • Embodiment 42 The method of any one of embodiments 29-40, wherein the antibody comprises a heavy chain variable (VH) region and a light chain variable (VL) region; wherein the VH region comprises the amino acid sequence of EVQLVQSGAEVKKPGATVKISCKVSGYTFTDYYIHWVQQAPGKGLEWMGRVNPDHGDSYYNQKFKDKATITADKSTDTAYMELSSLRSEDTAVYFCARNYLFDHWGQGTLVTVSS (SEQ ID NO: 9) ; and wherein the VL region comprises the amino acid sequence of DIQMTQSPSSVSASVGDRVTITCKASQDVGTAVAWYQQKPGKAPKLLIYWASIRHT GVPSRFSGSGSGTDFTLTISSLQPEDFATYYCHQFATYTFGGGTKVEIK (SEQ ID NO: 10) .
  • VH heavy chain variable
  • VL light chain variable
  • Embodiment 43 The method of embodiment 41 or embodiment 42, wherein the antibody is a human IgG antibody.
  • Embodiment 44 The method of embodiment 43, wherein the antibody is a human IgG1, IgG2, or IgG4 antibody.
  • Embodiment 45 The method of any one of embodiments 29-38, wherein the amino acid sequence of the heavy chain of the antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain of the antibody is shown in SEQ ID NO: 2.
  • Embodiment 46 The method of any one of embodiments 29-38, wherein the antibody-drug conjugate is Disitamab vedotin or a biosimilar thereof.
  • Embodiment 47 The method of any one of embodiments 29-46, wherein the average DAR(i.e., Drug-to-Antibody Ratio) value of the antibody-drug conjugate is any number from 2 to 7.
  • the average DAR(i.e., Drug-to-Antibody Ratio) value of the antibody-drug conjugate is any number from 2 to 7.
  • Embodiment 48 The method of embodiment 47, wherein the average DAR value is 4 ⁇ 0.5.
  • Embodiment 49 The method of any one of embodiments 29-48, wherein, prior to administration of the ADC, the patient has previously received one or more prior treatments comprising a chemotherapeutic agent, targeted therapy, immunotherapy, or endocrine therapy.
  • Embodiment 50 The method of embodiment 49, wherein, prior to administration of the ADC, the patient has received a taxane systemic therapy.
  • Embodiment 51 The method of embodiment 49, wherein, prior to administration of the ADC, the patient has received systemic therapy with trastuzumab or a biosimilar thereof at least once.
  • Embodiment 52 The method of any one of embodiments 29-51, wherein the ADC is administered intranasally, subcutaneously, intradermally, intramuscularly, or intravenously.
  • Embodiment 53 The method of any one of embodiments 29-52, wherein the ADC is administered at a dose of 2.0 mg/kg every 2 weeks.
  • Embodiment 54 The method of any one of embodiments 29-53, wherein the ADC is administered as a monotherapy.
  • Embodiment 55 The method of any one of embodiments 29-54, wherein administration of the ADC results in improved progression-free survival (PFS) of the patient, as compared to administration of capecitabine and lapatinib.
  • PFS progression-free survival
  • Embodiment 56 The method of any one of embodiments 29-55, wherein the ADC is administered in a pharmaceutical composition comprising the ADC and a pharmaceutically acceptable carrier.
  • Example 1 Overall data analysis of the treatment of breast cancer patients
  • the primary endpoint indicator of the study was PFS (progression-free survival) , which was evaluated for 6 weeks ( ⁇ 7 days) .
  • the trial has completed the enrollment of 200 patients for treatment.
  • Disitamab vedotin namely RC48-ADC (average DAR value: 4 ⁇ 0.5)
  • RC48-ADC average DAR value: 4 ⁇ 0.5
  • Lapatinib was administered at 1250 mg once a day, 21 days as one cycle; and Capecitabine was administered at a total daily dose of 2000 mg/m 2 for continuous 14 days and rest for 7 days, 21 days as one cycle.
  • ITT (Intention-To-Treat) analysis of the enrolled 200 patients is shown in Table 1.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • COX proportional hazards model used treatment group and actual stratification factors (number of previous chemotherapy lines received for advanced disease ( ⁇ 1 vs. 2) , and presence or absence of visceral organ metastasis (yes vs. no) ) as independent variables to calculate the hazard ratio of RC48-ADC relative to capecitabine+lapatinib, 95%CI, and P value.
  • COX proportional hazards model used treatment group as an independent variable to calculate the hazard ratio, 95%CI, and P value of RC48-ADC relative to capecitabine+lapatinib.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • the overall data analysis showed that when comparing the Disitamab vedotin experimental group and the control group (lapatinib+capecitabine) , the progression-free survival data were 9.3 months and 7.1 months, respectively. Compared with the control group, the experimental group did not show a statistically significant advantage in the overall sample of breast cancer patients.
  • This example is intended to compare the effect of the Disitamab vedotin treatment group and the control treatment group (lapatinib+capecitabine) on the progression-free survival (PFS) in the liver metastasis subgroup.
  • the control treatment group lapatinib+capecitabine
  • PFS progression-free survival
  • the data analysis of treatment outcomes showed (see Table 3) that the progression-free survival time (median) of the patients without liver metastasis in the Disitamab vedotin treatment group was 7.0 months, and the progression-free survival time (median) of the patients without liver metastasis in the control treatment group was 9.0 months.
  • the progression-free survival time (median) of the patients without liver metastasis in the Disitamab vedotin treatment group was shorter than that in the control treatment group by 2.0 months.
  • the progression-free survival time (median) of the patients with liver metastasis in the Disitamab vedotin treatment group was 12.5 months, and the progression-free survival time (median) of the patients with liver metastasis in the control treatment group was only 5.6 months.
  • the progression-free survival time (median) of the patients with liver metastasis in the Disitamab vedotin treatment group was significantly longer than the progression-free survival time (median) of the patients with liver metastasis in the control treatment group by 6.9 months.
  • the Disitamab vedotin treatment group can significantly improve the disease progression-free time and survival time of the patients with liver metastasis, which has extremely high clinical value.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • This example is intended to compare the effect of the Disitamab vedotin treatment group and the control treatment group (lapatinib+capecitabine) on the progression-free survival (PFS) in the lung metastasis subgroup.
  • the control treatment group lapatinib+capecitabine
  • PFS progression-free survival
  • the data analysis of treatment outcomes showed (see Table 4) that the median progression-free survival time of the patients with lung metastasis in the Disitamab vedotin treatment group was 8.2 months, and the progression-free survival time (median) of the patients with lung metastasis in the control treatment group was 8.3 months, there being no significant difference between the two.
  • the progression-free survival time (median) of the patients without lung metastasis in the Disitamab vedotin treatment group was 10.9 months
  • the progression-free survival time (median) of the patients without lung metastasis in the control treatment group was only 5.6 months.
  • the progression-free survival time (median) of the patients without lung metastasis in the Disitamab vedotin treatment group was significantly longer than the progression-free survival time (median) of the patients without lung metastasis in the control treatment group by 5.3 months.
  • the Disitamab vedotin treatment group can significantly improve the disease progression-free time and survival time of the patients, which has extremely high clinical value.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • This example is intended to compare the effect of the Disitamab vedotin treatment group and the control treatment group (lapatinib+capecitabine) on the progression-free survival (PFS) in the bone metastasis subgroup.
  • the control treatment group lapatinib+capecitabine
  • PFS progression-free survival
  • the data analysis of treatment outcomes showed (see Table 5) that the progression-free survival time (median) of the patients with bone metastasis in the Disitamab vedotin treatment group was 7.8 months, and the progression-free survival time (median) of patients with bone metastasis in the control treatment group was 6.0 months.
  • the progression-free survival time (median) of the patients with bone metastasis in the Disitamab vedotin treatment group was slightly longer than in the control treatment group, but there was no very significant difference between the two.
  • the progression-free survival time (median) of the patients without bone metastasis in the Disitamab vedotin treatment group was 9.6 months, and the progression-free survival time (median) of the patients without bone metastasis in the control treatment group was 8.0 months.
  • the progression-free survival time (median) of the patients without bone metastasis in the Disitamab vedotin treatment group was slightly longer than that in the control treatment group (by 1.6 months) , but there was no very significant difference between the two, and patient benefits were limited.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • This example is intended to compare the effect of the Disitamab vedotin treatment group and the control treatment group (lapatinib+capecitabine) on the progression-free survival (PFS) in the viscera metastasis subgroup (similarly hereinafter) .
  • the Disitamab vedotin treatment group including 77 cases with viscera metastasis and 22 cases without viscera metastasis; and there were 101 research subjects in the control treatment group, including 78 cases with viscera metastasis and 23 cases without viscera metastasis.
  • the data analysis of treatment outcomes showed (see Table 6) that the progression-free survival time (median) of the patients with viscera metastasis in the Disitamab vedotin treatment group was 9.3 months, and the progression-free survival time (median) of the patients with viscera metastasis in the control treatment group was 6.9 months.
  • the progression-free survival time (median) in the patients with viscera metastasis in the Disitamab vedotin treatment group was longer than that in the control treatment group by 2.4 months, but the hazard ratio test did not show statistical differences between the two.
  • the progression-free survival time (median) of the patients without viscera metastasis in the Disitamab vedotin treatment group was 9.6 months and the progression-free survival time (median) of the patients without viscera metastasis in the control treatment group was 8.1 months.
  • the progression-free survival time (median) of the patients without viscera metastasis in the Disitamab vedotin treatment group was slightly longer than that in the control treatment group (by 1.5 months) , but there was also no statistical difference shown between the two.
  • Percentage calculations are based on the total number of subjects in each subgroup in the ITT analysis set.
  • Table 7 presents a summary of the progression-free survival times in different metastatic site subgroups for the Disitamab vedotin treatment group and the control treatment group (lapatinib+capecitabine) . From the table, it was observed that:
  • the progression-free survival time in the Disitamab vedotin treatment group was 7.0 months, and the progression-free survival time in the capecitabine+lapatinib group was 9.0 months.
  • the progression-free survival time of the breast cancer patients without liver metastasis in the Disitamab vedotin treatment group was shorter by 2.0 months.
  • the progression-free survival time in the Disitamab vedotin treatment group was 12.5 months, and the progression-free survival time in the capecitabine+lapatinib group was 5.6 months.
  • the progression-free survival time in the Disitamab vedotin treatment group was 8.2 months, and the progression-free survival time in the capecitabine+lapatinib group was 8.3 months.
  • the progression-free survival time of the breast cancer patients with lung metastasis occurred in the Disitamab vedotin treatment group was shorter by 0.1 months.
  • the progression-free survival time in the Disitamab vedotin treatment group was 10.9 months and the progression-free survival time in the capecitabine+lapatinib group was 5.6 months.
  • the progression-free survival time in the Disitamab vedotin treatment group was 9.6 months, and the progression-free survival time in the capecitabine+lapatinib group was 8.0 months; and in breast cancer patients with bone metastasis, the progression-free survival time in the Disitamab vedotin treatment group was 7.8 months, and the progression-free survival time in the capecitabine+lapatinib group was 6.0 months.
  • the progression-free survival times in the Disitamab vedotin treatment groups were longer than that in the capecitabine+lapatinib groups, but the prolonged times had no statistically significant difference.
  • the progression-free survival time of the breast cancer patients with viscera metastasis in the Disitamab vedotin treatment group was 9.3 months, and the progression-free survival time in the capecitabine+lapatinib group was 6.9 months; and in breast cancer patients without viscera metastasis, the progression-free survival time in the Disitamab vedotin treatment group was 9.6 months, and the progression-free survival time in the capecitabine+lapatinib group was 8.1 months.
  • the progression-free survival times in the Disitamab vedotin treatment groups were longer than that in the capecitabine+lapatinib groups, but the prolonged times had no statistically significant difference.
  • Disitamab vedotin can effectively prolong the disease progression-free time and survival time of breast cancer patients with liver metastasis or breast cancer patients without lung metastasis, and the effect is non-obvious; i.e., the application of Disitamab vedotin in the subgroup of breast cancer patients with liver metastasis and breast cancer patients without lung metastasis has outstanding substantive features and significant progress, and can clinically produce huge social benefits and provide patients with more precise treatment options, thereby effectively reducing the overall medical burden of the society.

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CN202280015186.0A CN117460538A (zh) 2021-02-18 2022-02-17 靶向her2的抗体-药物结合物在治疗特异性乳腺癌中的用途
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