WO2022138347A1 - 高周波電気刺激の適用による血管機能改善方法 - Google Patents

高周波電気刺激の適用による血管機能改善方法 Download PDF

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Publication number
WO2022138347A1
WO2022138347A1 PCT/JP2021/046146 JP2021046146W WO2022138347A1 WO 2022138347 A1 WO2022138347 A1 WO 2022138347A1 JP 2021046146 W JP2021046146 W JP 2021046146W WO 2022138347 A1 WO2022138347 A1 WO 2022138347A1
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WIPO (PCT)
Prior art keywords
high frequency
skin
electrode
voltage
beauty
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Ceased
Application number
PCT/JP2021/046146
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English (en)
French (fr)
Japanese (ja)
Inventor
菜緒 板井
明子 高橋
麻呂 徳重
俊徳 栗田
健太朗 加治屋
謙太朗 山▲崎▼
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Shiseido Co Ltd
Ya Man Ltd
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Shiseido Co Ltd
Ya Man Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to JP2022572208A priority Critical patent/JPWO2022138347A1/ja
Priority to CN202180078656.3A priority patent/CN116546934A/zh
Publication of WO2022138347A1 publication Critical patent/WO2022138347A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A61B18/04—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating
    • A61B18/12—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating by passing a current through the tissue to be heated, e.g. high-frequency current
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents

Definitions

  • the present invention relates to a technique for improving vascular function by applying high-frequency electrical stimulation, for example, a beauty method.
  • a high frequency beauty method is known that can improve wrinkles and sagging of the skin by passing a high frequency (RF) current to the skin such as the face and limbs.
  • RF high frequency
  • a high-frequency current is applied to the skin, the temperature of the dermis layer and subcutaneous tissue rises. It is expected that the heating action promotes the flow of blood and promotes the regeneration of collagen in the dermis layer by causing heat damage to the collagen in the dermis layer.
  • various beauty treatment devices using such high frequencies have been proposed (for example, Patent Document 1).
  • Patent Document 1 Japanese Unexamined Patent Publication No. 2018-489
  • the conventional high-frequency cosmetology method promotes blood circulation and collagen regeneration based on the heating action of the dermis layer and subcutaneous tissue by high frequency, and is a temporary treatment. With increasing awareness of health in recent years, more fundamental improvement of function at the constitutional level is desired.
  • One of the objects of the present invention is to provide a cosmetic method based on the improvement of vascular function obtained by applying high frequency to the skin.
  • a cosmetological method comprising applying high frequency to the skin to enhance gene expression of a vascular structure stabilizing factor.
  • a method for enhancing gene expression of a vascular structure stabilizing factor which comprises a step of applying high frequency to the skin.
  • Yet another aspect of the invention provides a method of increasing the thickness of a blood vessel, comprising the step of providing high frequency to the skin.
  • a method of operating a high frequency beauty treatment apparatus having a pair of electrodes and a control circuit for applying a high frequency voltage to the pair of electrodes.
  • a high frequency voltage that enhances the gene expression of the vascular structure stabilizing factor is applied to the pair of electrodes.
  • the high frequency voltage is applied with the pair of electrodes in contact with the skin.
  • applying high frequency and “applying high frequency” mean applying a high frequency voltage to an object by applying a high frequency voltage between a pair of electrodes arranged in contact with the object at intervals. It means that a high frequency current is passed.
  • the word "about” used to indicate a numerical value means that it includes a numerical value of 5% before and after the numerical value modified by this word.
  • a cosmetological effect can be brought about by improving the vascular function.
  • a cosmetic method comprises applying a high frequency to the skin to enhance gene expression of a vascular structure stabilizing factor.
  • a vascular structure stabilizing factor By enhancing the gene expression of the vascular structure stabilizing factor, the vascular structure is stabilized (for example, the thickness of the blood vessel becomes thicker), and a strong blood vessel can be created. Since strong blood vessels contribute to the tension of the skin, it can be said that the application of RF current to the skin contributes to the tension of the skin.
  • the frequency of the high frequency applied to the skin is in the range of 0.5 MHz to 4 MHz, for example, 0.5 MHZ, 1 MHz, 2 MHZ, 3 MHz or 4 MHz, or the range defined by any of the above frequencies (eg, for example. Any frequency included in the range of 1 to 4 MHz) may be used. In some embodiments of the invention, the frequency is about 1 MHz.
  • VE-Cadherin and Integrin ⁇ 5 are known as vascular structure stabilizing factors.
  • VE-cadherin is a factor responsible for the adhesion between vascular endothelial cells.
  • Integrin ⁇ 5 is an important factor in the interaction between vascular endothelial cells and collagen. Therefore, the vascular structure stabilizing factor is preferably at least one factor selected from VE-cadherin and integrin ⁇ 5.
  • the application of high frequency to the skin may be combined with the application of drugs to the skin and various extracts.
  • applicable drugs and various extracts include inositol, retinol derivative, xylitol, hyaluronic acid, glycerin, yeast extract, cherry leaf extract, keihi extract, pine extract, bulgarian rose water, okra extract, ibukijakou extract, waremokou extract, and glycyrrhizin.
  • Dipotassium acid Dipotassium acid, Ginkgo biloba extract, Tencha extract, Yokuinin extract, Kanzo extract, Otaneninjin extract, Clara extract, Star fruit leaf extract, Carrot extract, Juyaku extract, Neem leaf extract, Saffron extract, Kina extract, Confree extract, Thyme extract, Fuyu strawberry , Yomena, Mube, Ryukyu Strawberry, Indian Yomena, Matebashii, Ogon Extract, Rosemary Extract, Anzu Nucleus Granules, Gentiana Extract, Hakka Powder, Taisou Extract, Hop Extract, Kiwi Extract, Roman Camille Extract, Apple Extract, Sanzashi Extract, Examples include Ukon extract, Yamamomo, Futomomo, and Konara. These can be used alone or in combination.
  • a high frequency beauty treatment device (hereinafter, also referred to as “high frequency device” or “beauty device”) can be used for applying high frequency to the skin.
  • high frequency device also referred to as “high frequency device” or “beauty device”
  • a form of the high frequency device will be described with reference to FIG.
  • the high frequency device 1 has an electrode unit 10 and a control circuit 20.
  • the electrode unit 10 has a first electrode 10a and a second electrode 10b connected to the control circuit 20.
  • a contact surface that comes into contact with the user's skin is provided on the electrode portion 10, and the first electrode 10a and the second electrode 10b are arranged at intervals from each other on the contact surface, whereby the first electrode 10a and the second electrode 10b are provided. They can be easily brought into contact with the skin at intervals.
  • the first electrode 10a and the second electrode 10b may be connected to the control circuit 20 via a wiring cable instead of the contact surface, whereby the first electrode 10a and the second electrode 10b can be freely arranged. can do.
  • the control circuit 20 can have a power supply unit, a voltage control unit, and a resistance measurement unit.
  • the power supply unit applies a high frequency voltage (voltage indicating a high frequency) between the first electrode 10a and the second electrode 10b.
  • the power supply unit has an oscillation circuit that generates a high frequency voltage, a booster circuit that boosts the oscillated voltage, and the like.
  • a high frequency voltage of about 0.5 MHz to 4 MHz is applied to the first electrode 10a and the second electrode 10b. It is configured to apply between.
  • the voltage control unit controls the high frequency voltage applied by the power supply unit by controlling the power supply unit. Further, the voltage control unit uses the measurement result by the resistance measurement unit for voltage control.
  • the resistance measuring unit measures the resistance value (electrical resistance value) between the first electrode 10a and the second electrode 10b when the first electrode 10a and the second electrode 10b are in contact with the user's skin.
  • This resistance value changes depending on the condition of the user's skin and the type of external skin preparation such as lotion applied to the skin. For example, it is known that when a high-frequency current is applied to the skin of a human body, the skin generates heat and the impedance inside the skin decreases as the temperature of the skin rises. Therefore, as the temperature of the skin rises, the current flowing between the first electrode 10a and the second electrode 10b increases, and the measured resistance value decreases.
  • the resistance measuring unit measures the resistance value at a predetermined time interval (for example, every 0.5 seconds), and supplies the measured resistance value to the voltage control unit each time.
  • the voltage control unit controls the high-frequency voltage according to the resistance value measured by the resistance measurement unit, for example, so that the larger the measured resistance value, the higher the high-frequency voltage (increasing the amplitude of the high-frequency voltage).
  • the lower limit of the high frequency voltage applied between the first electrode 10a and the second electrode 10b is preferably 50 Vpp or more, more preferably 70 Vpp or more in the peak voltage.
  • the upper limit of the high frequency voltage applied between the first electrode 10a and the second electrode 10b is preferably 100 V or less, more preferably 90 Vpp or less in the peak voltage.
  • the applied high frequency voltage can be, for example, 88 Vpp.
  • the application of the high frequency voltage may be continuous application or pulse application.
  • application (ON) and non-application (OFF) of high frequency voltage are periodically repeated.
  • the cycle of repeating the pulse application may be 0.1 seconds to 2 seconds, for example, 0.1 seconds, 0.2 seconds, 0.3 seconds, 0.5 seconds, 0.8 seconds, 1 second or 1 second. It can be .2 seconds.
  • the duty ratio which is the ON time ratio of the high frequency voltage in pulse application, is preferably in the range of 10% to 99%, and more preferably in the range of 20% to 80%.
  • the duty ratio can be, for example, 40%.
  • the application conditions of high frequency such as high frequency voltage and duty ratio may be set by the user.
  • the high frequency device may have an input device operated by the user.
  • any device such as a switch can be used.
  • the control circuit 20 accepts the input operation through the input device, and according to the received input operation, the high frequency voltage, continuous application or pulse application, and pulse application are performed.
  • Control parameters such as cycle and duty ratio.
  • each parameter may be set independently, or a plurality of combinations of high frequency voltage, period and duty ratio in pulse application are preset in the control circuit 20. The user may be able to select a preset combination.
  • the high frequency device By using the high frequency device as described above, the high frequency can be easily applied to the user's skin.
  • the high frequency device can be operated as follows. First, the first electrode 10a and the second electrode 10b are placed in contact with the user's skin at intervals from each other. Next, the control circuit 20 applies a high-frequency voltage between the first electrode 10a and the second electrode 10b arranged in contact with the user's skin so as to enhance the gene expression of the vascular structure stabilizing factor.
  • Intravascular cell adhesion includes adhesion between vascular endothelial cells and adhesion between vascular endothelial cells and extracellular matrix (collagen group, etc.), and the strength of these adhesions is the strength of the blood vessel itself. Is known to keep. In addition, it has been investigated in previous studies that this cell-cell adhesion decreases with aging and the capillaries become thinner.
  • vascular endothelial cells (HUVEC) as follows. First, vascular endothelial cells were seeded in a culture vessel (Petri dish) in which the first electrode was placed at the bottom. Next, the first electrode and the second electrode were placed in contact with the culture solution of the vascular endothelial cells in the culture vessel and at intervals from each other.
  • HAVEC vascular endothelial cells
  • a high frequency having a frequency of 1 MHz and a peak voltage of 88 Vpp was applied to the culture solution via the first electrode and the second electrode for 1 minute.
  • the high frequency was applied by applying a pulse with a repetition period of 1 second and a duty ratio of 40%.
  • the vascular endothelial cells were cultured for 24 hours in an incubator. After culturing, vascular endothelial cells were collected, RNA was extracted, and gene expression was confirmed by qPCR.
  • Experiment 1-1 and Experiment 1-2 are shown in FIG.
  • gene expression was confirmed under the same conditions as in Experiment 1-1 except that high frequency was not applied, and the result is also "control" in FIG. Also shown as.
  • the gene expression levels in Experiment 1-1 and Experiment 1-2 are shown as relative values when the gene expression level in the control is 1.
  • VE-cadherin the expression level was significantly increased by applying high frequency and as the application time was longer (Tukey's test showed a significant difference at the significance level of 5%).
  • integrin ⁇ 5 it cannot be said that it has increased significantly, but it can be said that it tends to increase due to the application of high frequency. From the above, it was shown that the application of high frequency enhances the adhesion factor of vascular endothelial cells and, as a result, contributes to the stabilization of vascular structure.
  • Experiment 2 Changes in vascular structure due to application of high frequency to surplus skin after surgery
  • the surplus skin for surgery was used as the subject, and the change in the vascular structure was confirmed by applying a high frequency with a frequency of 1 MHz and an inter-peak voltage of 88 Vpp as in Experiment 1.
  • the high frequency was applied for 3 minutes.
  • the high frequency was applied by applying a pulse with a repetition period of 1 second and a duty ratio of 40%.
  • high frequency was applied once a day, and changes in vascular structure were confirmed on the 1st, 3rd, and 5th days. Changes in the vascular structure were confirmed by visualizing the vascular structure.
  • FIG. 3A The images of the first day and the fifth day obtained by the experiment 2 are shown in FIG. 3A.
  • the vascular structure was visualized under the same conditions as above except that high frequency was not applied, and the result is also shown as "control" in FIG. 3A. From FIG. 3A, comparing the 1st day and the 5th day, it can be seen that the vascular structure of the skin to which the high frequency is applied is thin and thin, while the vascular structure is maintained in the skin to which the high frequency is applied. confirmed.
  • the thickness of blood vessels was measured on the 1st, 3rd, and 5th days of the skin to which high frequency was applied and the skin to which high frequency was not applied, respectively.
  • the number of blood vessels measured was 90 each.
  • the measurement results are shown in FIG. 3A. From the results shown in FIG. 3A, it was confirmed that the blood vessels tended to thicken by applying high frequency, and in particular, it was found that the effect was remarkable when the application of high frequency was continued regularly (according to Welch's test). On the 3rd day, a significant difference was observed at the significance level of 5%, and on the 5th day, a significant difference was observed at the significance level of 1%).

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PCT/JP2021/046146 2020-12-23 2021-12-14 高周波電気刺激の適用による血管機能改善方法 Ceased WO2022138347A1 (ja)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024154694A1 (ja) * 2023-01-18 2024-07-25 ヤーマン株式会社 肌処理装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013524946A (ja) * 2010-04-19 2013-06-20 ザ プロクター アンド ギャンブル カンパニー 哺乳類の皮膚の状態を調節するための、エネルギー及び局所適用組成物の併用
JP2018000489A (ja) * 2016-06-30 2018-01-11 株式会社メディカ ボーテ 美容機器
JP2018504976A (ja) * 2015-02-03 2018-02-22 ジョンジュ ナ 皮膚内の血管の治療装置
JP2018188365A (ja) * 2017-04-28 2018-11-29 ハザー株式会社 美容用組成物、美容処理装置および美容方法
WO2019098302A1 (ja) * 2017-11-17 2019-05-23 株式会社資生堂 ＶＥ－カドヘリン発現促進剤及び／又はインテグリンα５発現促進剤

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013524946A (ja) * 2010-04-19 2013-06-20 ザ プロクター アンド ギャンブル カンパニー 哺乳類の皮膚の状態を調節するための、エネルギー及び局所適用組成物の併用
JP2018504976A (ja) * 2015-02-03 2018-02-22 ジョンジュ ナ 皮膚内の血管の治療装置
JP2018000489A (ja) * 2016-06-30 2018-01-11 株式会社メディカ ボーテ 美容機器
JP2018188365A (ja) * 2017-04-28 2018-11-29 ハザー株式会社 美容用組成物、美容処理装置および美容方法
WO2019098302A1 (ja) * 2017-11-17 2019-05-23 株式会社資生堂 ＶＥ－カドヘリン発現促進剤及び／又はインテグリンα５発現促進剤

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024154694A1 (ja) * 2023-01-18 2024-07-25 ヤーマン株式会社 肌処理装置

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