WO2022114783A1 - Composition contenant un produit fermenté de miel de cactus et son utilisation pour améliorer l'état de la peau - Google Patents

Composition contenant un produit fermenté de miel de cactus et son utilisation pour améliorer l'état de la peau Download PDF

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WO2022114783A1
WO2022114783A1 PCT/KR2021/017422 KR2021017422W WO2022114783A1 WO 2022114783 A1 WO2022114783 A1 WO 2022114783A1 KR 2021017422 W KR2021017422 W KR 2021017422W WO 2022114783 A1 WO2022114783 A1 WO 2022114783A1
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skin
composition
cactus
honey
fermented product
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PCT/KR2021/017422
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English (en)
Korean (ko)
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이원희
이찬우
김면수
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엘리케이파크 주식회사
주식회사 미그림
(주)인터케어
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Publication of WO2022114783A1 publication Critical patent/WO2022114783A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/33—Cactaceae (Cactus family), e.g. pricklypear or Cereus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A61K8/988—Honey; Royal jelly, Propolis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • composition comprising a fermented product obtained by double fermenting cactus honey, mojave yucca, echinacea, or a combination of two or more thereof, and a use thereof for improving skin condition.
  • the skin is a primary barrier of the human body from the external environment and is one of the important organs of the human body that regulates body temperature, barrier function, and various physiological functions.
  • Human skin undergoes an aging process as it ages, and the causes can be largely divided into intrinsic aging and extrinsic aging.
  • Intrinsic aging is a process that cannot be avoided by anyone with aging, and wrinkles occur, the skin loses elasticity, moisture in the skin cells is lost, and the structure of the stratum corneum changes.
  • exogenous aging is caused by external stress such as environmental factors, and among them, ultraviolet rays are the main cause, also called photoaging.
  • Another phenomenon of skin aging is that the ability to retain moisture is reduced and the skin becomes dry and rough. Water constitutes about 70% of the human body and helps to keep the skin moist and shiny.
  • the stratum corneum of a healthy epidermis contains 15-20% of moisture, and when the level falls below 10%, an abnormality occurs in the skin barrier and the skin becomes dry and wrinkles increase.
  • the water content of the stratum corneum is determined by the sebum membrane, a lipid mixture produced and secreted by the epidermis, and natural moisturizing factor (NMF), a water-soluble component present in the stratum corneum.
  • NMF natural moisturizing factor
  • Hyaluronic acid one of the natural moisturizing factors, is formed in fibroblasts and keratinocytes, and as a component of point polysaccharide, it contains a lot of polysaccharides, so it has the characteristic that it can contain a considerable amount of moisture.
  • the cycle is 2 to 4.5 days, and by combining with water that is 1,000 times its own weight, it regulates the skin barrier function and hydrates the extracellular matrix to maintain the homeostasis of water in the tissue. Therefore, when the moisture content in the skin decreases and the amount of hyaluronic acid decreases, it is directly related to skin aging.
  • Hyaluronan synthase has HAS-1, 2, and 3, and glucuronic acid and N-acetylglucosamine are repeatedly added to polysaccharides in the initial stage of the synthetase to produce hyaluronic acid.
  • Aquaporin is present in the protein that regulates the movement of water.
  • Aquaporin-3 (AQP-3), which is mainly present in keratinocytes of the epidermis and is a water passage through which water, glycerol, urea, etc. move, provides smooth moisture between cells.
  • Patent Document 1 KR 10-1036933 B1
  • composition comprising a fermented product obtained by double fermenting cactus honey, mojave yucca, echinacea, or a combination of two or more thereof.
  • a method for preparing the fermented product is provided.
  • It provides a method for improving the skin condition of a subject, comprising administering to a subject in need thereof an effective amount of the fermented product or a composition comprising the same.
  • It provides a method for preventing or treating skin aging or premature aging, comprising administering an effective amount of the fermented product or a composition comprising the same to an individual in need thereof.
  • fermented product for use in preparing a composition for improving skin conditions.
  • fermented product for use in the manufacture of a medicament for the prevention or treatment of skin aging or premature aging.
  • composition comprising a fermented product obtained by double-fermenting cactus honey, moja yucca, echinacea, or a combination of two or more thereof.
  • double fermentation means performing the fermentation process twice.
  • the secondary fermentation may be performed on the primary fermented product obtained by primary fermenting the fermentation raw material.
  • the primary fermentation and secondary fermentation may be the same or different.
  • the primary fermentation and the secondary fermentation may be different from each other.
  • cactus honey, mojave yucca, echinacea, or a combination of two or more thereof is first fermented by lactic acid bacteria and the fermented product obtained by secondary fermentation by Saccaromyces sp. microorganisms.
  • a composition is provided.
  • the composition may be a composition for improving skin condition, improving skin beauty, preventing, improving or treating skin diseases.
  • the composition may be for improving skin condition.
  • the skin condition improvement may be skin aging prevention, skin wrinkle improvement, skin elasticity enhancement, skin moisturizing, skin barrier strengthening, or a combination of two or more thereof.
  • skin aging refers to the tangible and intangible changes that appear on the skin with age, such as a thinning of the epidermis, the number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle, wound healing, It refers to a decrease in skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage protection, chemical removal ability, immune response, sensory function, and thermoregulation.
  • the fermented product may be for preventing skin aging caused by extrinsic factors or intrinsic factors, improving skin aging, or for anti-aging.
  • the extrinsic factor refers to various external factors, such as ultraviolet light (light).
  • the intrinsic factor is also referred to as a chronological factor, and refers to a factor mainly caused by the passage of time. That is, the skin aging specifically refers to not only the premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemical substances, etc., but also a natural aging phenomenon that occurs as the proliferation of skin cells decreases with aging. , that is, it is a concept that includes skin aging due to age, and includes all of wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include those that cause wrinkles by stimulation by changes in internal and external factors by changing the components constituting the skin tissue.
  • the aging may be photoaging.
  • photoaging is a phenomenon induced by external environmental factors, and the most representative factor is ultraviolet rays.
  • UVB ultraviolet B
  • skin wrinkle improvement is interpreted to include not only an action of reducing the number or depth of skin wrinkles, but also inhibiting skin aging through wrinkle improvement.
  • the term “enhancement of skin elasticity” may refer to any action that increases skin elasticity, which is reduced due to aging, or prevents deterioration of skin elasticity.
  • moistureturizing the skin may refer to any action that retains moisture in the skin or prevents moisture loss.
  • the skin moisturizing effect can help improve skin wrinkles and increase elasticity.
  • stressening the skin barrier may refer to any action that enhances the function of the skin barrier, which is located at the outermost part of the skin and prevents loss of moisture and nutrients.
  • skin disease may be a disease caused by skin aging, premature aging, or damage to the skin barrier function.
  • prevention includes inhibiting the development of a disease.
  • treatment includes inhibiting, alleviating, or eliminating the development of a disease.
  • the skin aging may be photoaging or skin aging due to age.
  • progeria also called “Hutchinson-Gilford Progeria Syndrome” or “Gilford's syndrome” is a disease in which premature aging occurs in young children.
  • the damage to the skin barrier function may refer to any change that appears in the skin as the function of the skin barrier is reduced or damaged. For example, it may include increased skin wrinkles, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne, and the like.
  • the composition may inhibit the expression of progerin (Progerin).
  • Progerin is a protein associated with progeria, or Hutchinson-Gilford Progeria syndrome, and appears due to a defect in the LaminA protein. When progerin increases, the cell nucleus becomes unstable and the aging phenomenon occurs quickly.
  • the composition may exhibit an anti-aging effect by inhibiting the expression of progerin, or an effect of preventing or treating premature aging, such as progeria (or Hutchinson-Gilford Progeria syndrome).
  • the composition may increase the expression of SIRT1.
  • SIRT1 deacetylates histones and is involved in chromatin modification and gene silencing, etc., and is involved in lifespan extension effects. It has also been reported to be involved in inflammation and DNA damage responses.
  • the composition may exhibit an anti-aging effect by increasing the expression of SIRT1.
  • the composition may increase the expression of procollagen.
  • procollagen collagen related to wrinkles of skin cells is synthesized in the form of a precursor called procollagen. Therefore, by measuring the amount of the procollagen peptide, it is possible to grasp the degree of collagen synthesis in the cell.
  • the composition may exhibit the effect of improving skin wrinkles and enhancing skin elasticity by increasing the expression of procollagen.
  • the composition may increase the expression of collagen synthesis factor (Collagen, type 1, alpha 1, COL1A1). By measuring the expression level of COL1A1, the degree of collagen synthesis in the cell can be determined.
  • the composition induces collagen synthesis in the skin by increasing the expression of COL1A1, thereby improving skin wrinkles and enhancing skin elasticity.
  • the composition may increase the expression of aquaporin 3 (AQP3).
  • the composition may exhibit a skin moisturizing effect by smoothly supplying moisture to skin cells by increasing the expression of AQP3.
  • the composition may increase the expression of hyaluronan synthase 3 (HAS3).
  • HAS3 hyaluronan synthase 3
  • the composition may induce hyaluronic acid synthesis by increasing the expression of HAS3, thereby exhibiting skin moisturizing and skin barrier strengthening effects.
  • the composition may increase the expression of filaggrin.
  • the filaggrin is a moisturizing component that forms the skin barrier.
  • the composition may exhibit skin moisturizing and skin barrier strengthening effects by increasing the expression of filaggrin.
  • composition may inhibit the expression of progerin or increase the expression of any one or more of SIRT1, procollagen, COL1A1, AQP3, HAS3, and filaggrin.
  • the fermented product is excellent in preventing skin aging, preventing or treating premature aging, improving skin wrinkles, enhancing skin elasticity, moisturizing the skin, and strengthening the skin barrier.
  • the fermented product is cactus honey, mojave yucca, echinacea, or a combination of two or more thereof by primary fermentation by Lactobacillus sp. microorganisms and secondary by Saccaromyces sp. microorganisms It can be obtained by fermentation.
  • the cactus honey, mojave yucca, echinacea, or a combination of two or more thereof is i) a cactus honey extract, a mojave yucca extract, or an echinacea extract, or ii) cactus honey, mojave yucca, and echinacea an extract of the combination, or iii) an extract of a combination of cactus honey, mojave yucca, and echinacea.
  • cactus is a generic term for dicotyledonous plants belonging to the cactus family.
  • the cactus is not limited to a specific type, but may be, for example, a bogum cactus ( Opuntia Ficus-Indica ), a marginal cactus ( Carnegiea gigantea ), a pre-clear cactus ( Opuntia engelmannii , Engelmann Prickly Pear Cactus ), and the like.
  • the bogum cactus is also called baeknyeoncho.
  • the marginal cactus is also called Saguaro cactus.
  • cactus honey is honey harvested from cacti.
  • the cactus nectar may be nectar obtained from cactus flowers or cactus fruits.
  • the cactus honey may be bogum cactus honey or alfalfa cactus honey, but is not limited to these specific types.
  • the cactus honey may be cactus honey collected from a farm in Sonora, Arizona, USA, but is not limited to this specific type.
  • the "cactus honey extract” may be an extract obtained by extracting cactus honey using any extraction solvent.
  • the cactus honey extract may be an extract extracted using water as an extraction solvent.
  • Mojave yucca Yucca schidigera
  • Bojave yucca is widely distributed throughout the southeastern United States, with Arizona being the largest distribution center for this plant. Mojave yucca has long been the most important resource used by the indigenous peoples of southeastern North America, and all parts, including stems, leaves, flowers, fruits, and roots, have been used for food. In particular, the root was used as a material for making soap, and Native Americans used it to prevent dandruff and hair loss, and it has also been used for a long time to treat headaches, bleeding, gonorrhea, arthritis, and rheumatism.
  • the “mojave yucca extract” may be an extract obtained by extracting mojave yucca using any extraction solvent or a commercially available product.
  • the Mojavy yucca extract may be an extract extracted using water as an extraction solvent.
  • the extraction site of the mojave yucca is not limited to a specific site.
  • the mojave yucca extract may be a mojave yucca leaf/root/stem extract (Yucca Schidigera Leaf/Root/Stem Extract) extracted from mojave yucca leaves, roots, and stems.
  • Echinacea angustifolia is a plant of the Asteraceae family. Echinacea is native to North America and is the most commonly used medicinal herb in the world. As a folk herb used by Native Americans, it is known to be effective in relieving pain and treating toothache, as well as in treating sore throat. Echinacea has echinacoside as its main ingredient, and in addition, it contains caffeic acid derivatives and flavonoids to enhance immunity, anti-inflammatory, and anti-viral effects.
  • the "Echinacea Angustifolia Extract” may be an extract obtained by extracting Echinacea using any extraction solvent or a commercially available product. In one embodiment, the echinacea extract may be an extract extracted using water as an extraction solvent. The extraction site of the echinacea is not limited to a specific site. In one embodiment, the echinacea extract may be an echinacea whole plant extract.
  • the fermented product can be obtained by primary fermentation of cactus honey extract by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces.
  • the fermented product may be obtained by primary fermentation of the Mojaveyucca extract by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces.
  • the fermented product may be obtained by primary fermentation of the Echinacea extract by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces.
  • the fermented product may be obtained by primary fermentation of a combination of cactus honey, mojave yucca, and echinacea by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces.
  • the fermented product may be obtained by fermenting a mixture or extract containing cactus honey, moja yucca, and echinacea.
  • Cactus honey, mojave yucca, or echinacea used as a fermentation raw material for the primary fermentation may use a dried product of pulverizing and drying the raw material.
  • the mixing weight ratio of the cactus honey, mojave yucca, and echinacea is not limited to a specific weight ratio.
  • the fermented product is cactus honey, mojave yucca, and echinacea in a weight ratio of 1 to 100:1 to 100:1 to 100, for example, 1 to 50:1 to 50:1 to 50 by weight, 1 to 20: 1 ⁇ 20:1 ⁇ 20 weight ratio, 1 ⁇ 10:1 ⁇ 10:1 ⁇ 10 weight ratio, 1 ⁇ 5:1 ⁇ 5:1 ⁇ 5 weight ratio, 1-2:1 ⁇ 2:1 ⁇ 2 It may be obtained by fermenting a mixture including a weight ratio, or about 1:1:1 by weight.
  • the fermented product obtained by fermenting a mixture containing cactus honey, mojave yucca, and echinacea in a weight ratio of 1:1:1 is cactus honey, mojave yucca, or echinacea alone, or other It was confirmed that the skin aging prevention, progeria prevention or treatment, skin wrinkle improvement, skin elasticity enhancement, skin moisturizing, and skin barrier strengthening effects were superior compared to the case of using the weight ratio.
  • the primary fermentation may be performed in water.
  • the primary fermentation may be fermenting a mixture of cactus honey, mojave yucca, echinacea, or a combination of two or more thereof and water.
  • the water may be water rich in natural mineral components.
  • the water may be neutral to slightly alkaline water.
  • the water may have a pH of 7 to 8.
  • the water may be water that does not contain a sodium component. Since the water does not contain a sodium component, it may be advantageous for culturing microorganisms compared to general hot spring water.
  • a weakly acidic condition eg, pH 4.5 to 5.5
  • the mixing weight ratio of 'cactus honey, moja yucca, echinacea, or a combination of two or more thereof' and 'water' may be 3:7 to 1:9, but is not limited thereto.
  • the lactic acid bacteria may be probiotic lactic acid bacteria.
  • the lactic acid bacteria may be Lactobacillus sp. microorganisms or Bifidobacterium sp. microorganisms.
  • the Lactobacillus genus ( Lactobacillus sp.)
  • the microorganism is not limited to a specific type, for example, Lactobacillus acidophilus ( L. acidophilus ), Lactobacillus casei ( L. casei ), Lactobacillus gasseri ( L gasseri ), Lactobacillus Delbruecki bulgaricus ( L. delbrueckii ssp. bulgaricus ), Lactobacillus helveticus ( L. helveticus ), Lactobacillus fermentum ( L. fermentum ), Lactobacillus paracasei ( L. paracasei ) ), Lactobacillus plantarum ( L.
  • Lactobacillus reuteri L. reuteri
  • Lactobacillus rhamnosus L. rhamnosus
  • Lactobacillus salivarius L. salivarius It may be selected from the group consisting of have.
  • the Bifidobacterium genus ( Bifidobacterium sp.) microorganisms are not limited to a specific type, but, for example, Bifidobacterium bifidum ( B. bifidum ), Bifidobacterium brev ( B. breve ), Bifidobac It may be selected from the group consisting of terium longum ( B. longum ), and Bifidobacterium animalis lactis ( B. animalis ssp. lactis ).
  • the Saccaromyces genus ( Saccaromyces sp.)
  • the microorganism is not limited to a specific type, for example, Saccharomyces cerevisiae ( S. cerevisiae ) may be.
  • an appropriate temperature and time may be selected according to the type of microorganism.
  • the fermentation may be performed at 20 to 40° C., but is not particularly limited thereto.
  • the fermentation may be performed for 1 to 72 hours, or 24 to 72 hours, but is not particularly limited thereto.
  • the fermented product is a fermentation broth obtained by primary fermentation of cactus honey, moja yucca, echinacea, or a combination of two or more thereof by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces, a dilution or concentrate of the fermentation broth, It may include a dried product obtained by drying the fermentation broth, a crude product or a purified product thereof, a fraction obtained by fractionating the product, and the like.
  • the fermented product is 0.0001 to 99.9999% by weight, 0.0001 to 90% by weight, 0.0001 to 50% by weight, 0.0001 to 10% by weight, 0.01 to 99.9999% by weight, 0.01 to 90% by weight, 0.01 to 50% by weight based on the total weight of the composition , 0.01 to 10% by weight, 0.1 to 99.9999% by weight, 0.1 to 90% by weight, 0.1 to 50% by weight, or may be included in 0.1 to 10% by weight, but is not particularly limited thereto.
  • the fermented product is 0.0001 to 99.9999% (v / v), 0.0001 to 90% (v / v), 0.0001 to 50% (v / v), 0.0001 to 10% (v / v), 0.01 based on the total weight of the composition to 99.9999% (v/v), 0.01 to 90% (v/v), 0.01 to 50% (v/v), 0.01 to 10% (v/v), 0.1 to 99.9999% (v/v), 0.1 to 90% (v/v), 0.1 to 50% (v/v), 0.1 to 10% (v/v), or 0.1 to 1% (v/v), but is not particularly limited thereto.
  • the term "included as an active ingredient” means that the fermented product of the present specification is added to an extent capable of exhibiting the above-mentioned effects. In addition, this may include being formulated in various forms by adding various components as sub-components for drug delivery and stabilization.
  • the composition may be a cosmetic composition.
  • the cosmetic composition may be prepared in any conventionally prepared formulation.
  • the cosmetic composition may be a lotion, cream, essence, cleansing foam, cleansing water, pack, ampoule, body lotion, body oil, body gel, shampoo, conditioner, hair conditioner, hair gel, foundation, lipstick, mascara, makeup It may have a cosmetic formulation of a base or skin adhesion type.
  • the skin adhesion type cosmetic formulation may be, for example, a mask pack, but is not limited thereto.
  • Components included in the cosmetic composition may include components commonly used in cosmetic compositions in addition to the composition as an active ingredient, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and fragrances. may include
  • composition may be a composition for external application to the skin.
  • the external preparation for skin may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug-containing bandage, lotion, or a combination thereof.
  • the external preparation for skin is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, an aqueous component, an oily component, a powder component, alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, a preservative, an antioxidant, a surfactant, a fragrance , colorant, various skin nutrients, or a combination thereof may be appropriately formulated as needed.
  • the external preparation for skin includes metal-blocking agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belapamil, licorice extract, glablidine, and kaline.
  • metal-blocking agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belapamil, licorice extract, glablidine, and kaline.
  • Fruit hot water extracts, various herbal medicines, tocopherol acetate, glitylittic acid, tranexamic acid and derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can be mix
  • the composition may be a food composition.
  • the composition may be a health functional food composition.
  • the food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to a conventional method.
  • the mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prophylactic, health or therapeutic treatment).
  • the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material.
  • the beverage composition may contain various flavoring agents or natural carbohydrates as an additional component like a conventional beverage.
  • the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • sweetener natural sweeteners such as taumartin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used.
  • the health food composition can also be added to nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonated beverages carbonation agent used, or a combination thereof.
  • the health functional food composition may also contain natural fruit juice, fruit juice beverage, fruit flesh for the production of vegetable beverage, or a combination thereof.
  • the composition may be a pharmaceutical composition.
  • the pharmaceutical composition may be for preventing or treating skin aging or premature aging.
  • the skin aging may be photoaging or skin aging due to age.
  • the pharmaceutical composition may further include a pharmaceutically acceptable diluent or carrier.
  • the diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and the lubricant may be magnesium stearate, talc, or a combination thereof.
  • the carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof.
  • the excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof.
  • the disintegrant may be carboxymethyl cellulose calcium, sodium starch glycolate, anhydrous calcium monohydrogen phosphate, or a combination thereof.
  • the binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof.
  • the lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.
  • the pharmaceutical composition may be formulated as an oral or parenteral dosage form.
  • Oral dosage forms may be granules, powders, solutions, tablets, capsules, dry syrups, or a combination thereof.
  • the parenteral dosage form may be an injection.
  • Another aspect provides a method for preparing a fermented product of cactus honey, mojave yucca, echinacea, or a combination of two or more thereof.
  • the method comprises the steps of: primary fermentation of cactus honey, mojave yucca, echinacea, or a combination of two or more thereof with lactic acid bacteria; and performing secondary fermentation of the primary fermentation product with a Saccaromyces sp. microorganism to obtain a secondary fermentation product.
  • the method may be a method for producing a fermented cactus honey comprising the step of primary fermentation of a cactus honey extract by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces to obtain a fermented product have.
  • the method may be a method of producing a fermented Mojave yucca, comprising the step of primary fermentation of the Mojave yucca extract by lactic acid bacteria and secondary fermentation by a microorganism of the genus Saccharomyces to obtain a fermented product have.
  • the method is a method for producing a fermentation product of Echinacea comprising the step of primary fermentation of the Echinacea extract by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces to obtain a fermented product can
  • the method comprises the step of primary fermentation of a combination of cactus honey, moja yucca, and echinacea by lactic acid bacteria and secondary fermentation by microorganisms of the genus Saccharomyces to obtain a fermented product. It may be a method for preparing a co-fermented product of honey, mojave yucca, and echinacea. Details regarding the mixing weight ratio of the cactus honey, mojave yucca, and echinacea are the same as described above.
  • the primary fermentation may be performed in water.
  • the primary fermentation may be fermenting a mixture of cactus honey, mojave yucca, echinacea, or a combination of two or more thereof and water. Details regarding the water are the same as described above.
  • the mixing weight ratio of 'cactus honey, moja yucca, echinacea, or a combination of two or more thereof' and 'water' may be 3:7 to 1:9, but is not limited thereto.
  • the lactic acid bacteria and microorganisms of the genus Saccharomyces may be used in sufficient amounts to enable fermentation, respectively, which may be appropriately selected by those skilled in the art in consideration of the amount of fermentation raw materials.
  • the fermentation temperature and time may be selected sufficient temperature and time for fermentation to occur, which may be appropriately selected by those skilled in the art in consideration of the amount of fermentation raw material, the type of microorganism, and the like.
  • the primary fermentation or secondary fermentation may be performed at 20 to 40° C., but is not particularly limited thereto.
  • the primary or secondary fermentation may be performed for 1 to 72 hours, or 24 to 72 hours, but is not particularly limited thereto.
  • the method may further include filtering the primary fermented product before secondary fermentation of the primary fermented product.
  • the filtration may use a known conventional method.
  • the method after obtaining the secondary fermented product, may further include the step of filtering the secondary fermented product.
  • the filtration may use a known conventional method.
  • the method may further include deodorizing and/or decolorizing the secondary fermentation product.
  • the deodorization and/or decolorization may be performed using an adsorbent.
  • an adsorbent a known conventional adsorbent may be used, for example, activated carbon, zeolite, and the like, but is not particularly limited thereto.
  • Another aspect provides a method for improving a skin condition in a subject comprising administering to a subject in need thereof an effective amount of the fermented product or a composition comprising the same.
  • Another aspect provides a method for preventing or treating skin aging or premature aging, comprising administering to an individual in need thereof an effective amount of the fermented product or a composition comprising the same.
  • administering means the arrangement of the composition according to
  • Administration may be administered by methods known in the art. Administration can be administered directly to a subject by any means, for example, intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. can The administration may be systemically or locally. The administration may be applied to the skin.
  • the subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat.
  • the subject may be an individual in need of improving skin condition, for example, preventing skin aging, improving skin wrinkles, enhancing skin elasticity, moisturizing skin, strengthening skin barrier, preventing or treating premature aging.
  • the administration is 0.1 mg to 1,000 mg, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1 mg of the composition according to one embodiment per day 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered.
  • the dosage may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and reaction sensitivity, and those skilled in the art
  • the dosage may be appropriately adjusted in consideration of these factors.
  • the number of administration may be once a day or twice or more within the range of clinically acceptable side effects, and may be administered to one or two or more sites for the administration site, and total daily or at intervals of 2 to 5 days
  • the number of days of administration may range from 1 to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period.
  • the dose is the same as that of a human per kg, or the above dose is converted, for example, by the volume ratio (for example, average value) of the target animal and the organ (heart, etc.) of the human One dose can be administered.
  • Another aspect provides the use of the fermented product for use in preparing a composition for improving skin conditions.
  • Another aspect provides the use of the fermented product for use in the manufacture of a medicament for the prevention or treatment of skin aging or premature aging.
  • composition comprising cactus honey, mojave yucca, echinacea, or a double fermented product of two or more combinations thereof according to an aspect prevents skin aging, prevents or treats premature aging, improves skin wrinkles, improves skin elasticity, moisturizes skin, skin barrier It can be usefully used for prevention, improvement, or treatment of skin-related conditions such as strengthening.
  • 1 is a result of measuring progerin production in neonatal fibroblasts.
  • 5 is a result showing the relative expression level of COL1A1 mRNA.
  • 6 is a result showing the relative expression level of AQP-3 mRNA.
  • Fermentation raw materials were prepared in a weight ratio as shown in Table 1 below. Fermentation raw materials were pulverized and dried dried products of each raw material of cactus honey, mojave yucca, and echinacea. As the cactus honey, cactus honey collected from a farm in Sonora, Arizona, USA was used.
  • Example 1 a mixture containing cactus honey, mojave yucca, and echinacea in a weight ratio of 1:1:1 was used as a fermentation raw material.
  • Example 2 cactus honey alone was used as a fermentation raw material.
  • Example 3 Mojave Yucca alone was used as a fermentation raw material.
  • Example 4 echinacea alone was used as a fermentation raw material.
  • Example 5 a mixture containing cactus honey, mojave yucca, and echinacea in a weight ratio of 1:0.5:0.5 was used as a fermentation raw material.
  • the method for preparing the fermented product is as follows. Fermentation raw materials and water (product name: Purely SEDONA Water, manufacturer: Sedona Bottling Compony, Arizona, USA) are mixed in a weight ratio of 3:7 to 1:9, and Lactobacillus rhamnosus as a lactic acid bacterium Inoculated 30 to 40 Primary fermentation was performed at °C for about 72 hours. After filtering the primary fermentation, Saccaromyces cerevisiae was inoculated and secondary fermentation was performed at 20 to 30° C. for about 48 hours. After the fermentation was completed, it was filtered through 1 um and 0.45 um filters to obtain a double fermented product.
  • the water used in the primary fermentation is rich in natural mineral components, is neutral to weak alkaline water at a pH of 7 to 8, and does not contain sodium components, so it is advantageous for culturing microorganisms compared to general hot spring water.
  • a weak acidity ie, pH 4.5 to 5.5
  • Example 1 Example 2
  • Example 3 Example 4
  • Example 5 cactus honey One One - - One Mojave Yucca One - One - 0.5 Echinacea One - - One 0.5
  • the fermented products of Examples 1 to 5 were added at a concentration of 0.5% (v/v), respectively, and ELISA assay was performed. It was carried out to confirm the production of progerin. The group to which no fermented product was added was used as a negative control group.
  • 1 is a result of measuring progerin production in neonatal fibroblasts.
  • progerin production was higher in adult fibroblasts than in neonatal fibroblasts.
  • the fermented products of Examples 1 to 5 were added, there was an effect of reducing progerin production in both neonatal fibroblasts and adult fibroblasts.
  • the fermented product of Example 1 using cactus honey, mojave yucca, and echinacea in a weight ratio of 1:1:1 reduced progerin synthesis by about 20% in neonatal fibroblasts compared to the negative control group, and adult fibroblasts The effect was the best by reducing the synthesis of progerin by about 40% compared to the negative control group.
  • SIRT1 an anti-aging longevity gene
  • the fermented products of Examples 1 to 5 were pretreated at a concentration of 0.5% (v/v) for 1 hour, and UV of 20 mJ/cm 2 was applied. It was irradiated and reacted for 24 hours. After completion of the reaction, cells were harvested, proteins were separated through SDS-PAGE, and transferred to a polyvinylidene fluoride (PVDF) membrane. Thereafter, the primary antibody was reacted at room temperature for 1 hour using a monoclonal mouse anti-SIRT1 antibody (Sigma), and the secondary antibody was reacted for 1 hour using a goat anti-mouse IgG-HRP (Santa Cruz).
  • PVDF polyvinylidene fluoride
  • the membrane was washed 3 times with 1X TBS-T buffer and reacted with ECL (enhanced chemiluminescence) solution.
  • the protein was identified as a band using Chemi-Doc and quantified in comparison with ⁇ -actin through the image J program.
  • the group not irradiated with UV and not treated with the fermented products of Examples 1 to 5 was used as a positive control group, and the group irradiated with UV of 20 mJ/cm 2 was used as a negative control group.
  • human fibroblasts Human Fibroblast
  • the fermented products of Examples 1 to 5 were added at a concentration of 0.5% (v/v), and the relative production rate of procollagen was confirmed by ELISA assay.
  • a group to which no fermentation was added was used as a negative control group, and a group to which 10 ng/ml of TGF- ⁇ was added was used as a positive control group.
  • FIG. 5 is a result showing the relative expression level of COL1A1 mRNA. As shown in FIG. 5, when the fermented products of Examples 1 to 5 were added, the expression of COL1A1 was increased in both cases. In particular, it was confirmed that the fermented product of Example 1 increased the expression of COL1A1 by about 1.38 times compared to the negative control group.
  • the fermented products of Examples 1 to 5 were added at a concentration of 0.5% (v/v).
  • mRNA was obtained and AQP-3, HAS3, and filaggrin expression levels were compared and analyzed by real-time PCR.
  • the group to which no fermentation was added was used as a negative control group, and the group to which 1 uM of retinoic acid was added was used as a positive control group.
  • the primer sequences used in the PCR are shown in Table 3 below.
  • 6 is a result showing the relative expression level of AQP-3 mRNA.

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Abstract

La présente invention concerne une composition comprenant un produit fermenté obtenu par double fermentation de miel de cactus, Yucca schidigera, Echinacea, ou une combinaison de deux ou plus de ceux-ci, et son utilisation pour améliorer l'état de la peau. La composition peut être utilisée pour prévenir, améliorer ou traiter des états liés à la peau tels que la prévention du vieillissement de la peau, la prévention ou le traitement de la progéria, l'amélioration des rides de la peau, l'amélioration de l'élasticité de la peau, l'hydratation de la peau, le renforcement de la barrière cutanée, etc.
PCT/KR2021/017422 2020-11-30 2021-11-24 Composition contenant un produit fermenté de miel de cactus et son utilisation pour améliorer l'état de la peau WO2022114783A1 (fr)

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KR20170019830A (ko) * 2015-08-13 2017-02-22 인코스(주) 비누풀, 유카, 퀼라야 추출물이 함유된 천연계면활성제 조성물 및 이를 함유하는 화장료 조성물
KR20180063394A (ko) * 2016-12-01 2018-06-12 이현기 벌꿀 발효물을 함유한 각질제거 및 주름개선용 화장료 조성물
KR20190119033A (ko) * 2016-12-22 2019-10-21 엘브이엠에이취 러쉐르쉐 위쌍 블랙 비의 로얄 젤리를 포함하는 화장료 조성물

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Publication number Priority date Publication date Assignee Title
JPH09328410A (ja) * 1996-06-06 1997-12-22 Yushiro Chem Ind Co Ltd 化粧料及びその製造方法
KR20160092569A (ko) * 2015-01-27 2016-08-05 인코스(주) 효모, 유산균 2단 발효 추출물을 함유하는 항산화, 주름개선, 보습 효과를 갖는 화장료 조성물
KR20160119967A (ko) * 2015-04-07 2016-10-17 유씨엘 주식회사 에키네시아 추출물을 함유하는 화장료 조성물
KR20170019830A (ko) * 2015-08-13 2017-02-22 인코스(주) 비누풀, 유카, 퀼라야 추출물이 함유된 천연계면활성제 조성물 및 이를 함유하는 화장료 조성물
KR20180063394A (ko) * 2016-12-01 2018-06-12 이현기 벌꿀 발효물을 함유한 각질제거 및 주름개선용 화장료 조성물
KR20190119033A (ko) * 2016-12-22 2019-10-21 엘브이엠에이취 러쉐르쉐 위쌍 블랙 비의 로얄 젤리를 포함하는 화장료 조성물

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