WO2023149766A1 - Composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen - Google Patents

Composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen Download PDF

Info

Publication number
WO2023149766A1
WO2023149766A1 PCT/KR2023/001653 KR2023001653W WO2023149766A1 WO 2023149766 A1 WO2023149766 A1 WO 2023149766A1 KR 2023001653 W KR2023001653 W KR 2023001653W WO 2023149766 A1 WO2023149766 A1 WO 2023149766A1
Authority
WO
WIPO (PCT)
Prior art keywords
skin
extract
saffron
cosmetic composition
saffron flower
Prior art date
Application number
PCT/KR2023/001653
Other languages
English (en)
Korean (ko)
Inventor
정수경
경서연
김선영
장유진
윤석균
강승현
김영희
김연준
박명삼
Original Assignee
코스맥스 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 코스맥스 주식회사 filed Critical 코스맥스 주식회사
Publication of WO2023149766A1 publication Critical patent/WO2023149766A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat

Definitions

  • It relates to a cosmetic composition.
  • the skin is largely divided into three layers of tissues, epidermis, dermis, and hypodermis, in order from the outside, and functions to protect the human body from physical and chemical external environmental stimuli.
  • the epidermis is further divided into the stratum corneum, the granular layer, the prickle layer, and the basal layer.
  • the stratum corneum contains about 10 to 20% of water and exists in the outermost part of the human body, suppressing the evaporation of moisture to the outside of the body and excessive substances from the outside. It acts as a barrier to penetration.
  • the surface of the stratum corneum is covered with a thin natural protective film made of sebum from the sebaceous glands and sweat from the sweat glands to prevent evaporation of moisture.
  • One aspect is to provide a cosmetic composition for improving skin conditions comprising a saffron flower extract and a Danshen root extract as active ingredients.
  • Another aspect is to provide a composition for external application for skin for improving skin conditions comprising a saffron flower extract and a Danshen root extract as active ingredients.
  • Another aspect is to provide a health functional food composition for improving skin conditions comprising a saffron flower extract and a Danshen root extract as active ingredients.
  • compositions for improving skin conditions comprising a saffron flower extract and a Danshen root extract as active ingredients.
  • the saffron ( Crocus Sativus ) is a dried spice prepared by extracting the pistil of a flower, and includes compounds such as crocin, picrocrocin, safranal, and crocetin. are doing Among the compounds, crocin is a glycoside mixture that gives saffron a yellow-red color, and the main component of crocin is ⁇ -crocin. Generally, saffron flowers are discarded after removing the stigma. In particular, saffron flowers, like pistils, have a deep red color due to the crocin component, so there is a risk of discoloration, so it is difficult to use them as cosmetic ingredients at high concentrations.
  • saffron flowers specifically saffron petal extract, reduce the expression of MMP-1, which is increased by ultraviolet rays in skin fibroblasts, increase the expression of collagen, and promote cell migration in aged fibroblasts, thereby causing skin wounds. It was confirmed that not only can treat, but also exhibits a skin regeneration effect. Therefore, the saffron flower not only contains various active ingredients effective for improving skin beauty, but also has a lower cost compared to pistils, so it can be used as an alternative raw material.
  • Salvia Miltiorrhiza is a perennial herbaceous plant belonging to the Lamiaceae family, and is similar to ginseng in shape and is known to have a reddish color, promote blood circulation in the human body, and have excellent cholesterol-lowering effects.
  • the saffron flower extract is obtained by extracting saffron flowers with water, C1 to C4 lower alcohol, or a mixture thereof as a solvent, and the saffron flower extract may contain a crocin compound. After cutting the saffron flowers, it may be extracted by adding 2 to 20 times the weight of the saffron flowers with water, alcohol, or a mixture thereof. In addition, the extraction may be performed at 30 ° C to 100 ° C, and may be extracted for 1 to 10 hours.
  • the extraction method may use cold needle extraction, ultrasonic extraction, or reflux cooling extraction method, and the number of extractions may be performed for 1 to 5 times.
  • the saffron flower extract may be obtained by extracting the saffron flower with a mixture of water and a carbonhydrase as a solvent.
  • carbohydrates have a high molecular weight and are insoluble, there is a problem in that they are difficult to absorb into the body.
  • the carbolytic enzyme catalyzes a reaction that converts carbohydrates present in saffron flowers into monosaccharides such as glucose and maltose. Therefore, the saffron flower extract according to one embodiment contains a monosaccharide having a low molecular weight to promote skin absorption of active ingredients of the saffron flower, thereby maximizing the skin condition improvement effect.
  • the saffron flower extract may be obtained by extracting saffron flowers with a mixture of water and a carbonhydrase as a solvent and decolorizing the saffron flowers by chemical, physical, electrical or biological methods.
  • the decolorization may be performed by a filtration method using a filtering agent. Since the crocin compound is removed by the decolorization, there is no risk of discoloration, so it can be used in cosmetics at a high concentration.
  • the composition according to one embodiment may selectively apply a step of decolorizing the crocin compound according to the content of the saffron flower extract.
  • the composition may have skin barrier strengthening, skin wrinkles, skin elasticity or moisturizing effects.
  • skin aging refers to both tangible and intangible changes that appear in the skin with age, such as thinning of the epidermis, number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle, It refers to reduction in wound healing, skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical substance elimination ability, immune response, sensory function, and thermoregulation.
  • the composition may be used to improve skin aging caused by exogenous factors or endogenous factors.
  • the extrinsic factor refers to various external factors, such as ultraviolet rays (light), and the endogenous factor is also referred to as a chronological factor and refers to a factor mainly caused by the passage of time. That is, the skin aging is not only premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemicals, etc., but also a natural aging phenomenon that occurs as the proliferation of skin cells decreases with age. It is a concept that includes wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include stimulation caused by changes in internal and external factors that cause wrinkles by changing components constituting skin tissue.
  • the aging may be photoaging.
  • photoaging is a phenomenon caused by external environmental factors, and the most representative factor is ultraviolet rays. Ultraviolet rays cause damage to biocomponents such as activation of proteolytic enzymes, chain scission of matrix proteins, and abnormal cross-linking, and repetition of these mechanisms results in apparent skin aging.
  • wrinkle refers to a state in which elasticity of the skin is lost and loosened, and for example, the skin may be folded.
  • prevention or improvement of skin wrinkles may mean any action of preventing or improving wrinkles by suppressing the expression of wrinkle-related factors or increasing the total amount of collagen.
  • the "strengthen the skin barrier” may refer to any action that enhances the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients.
  • Impaired skin barrier function may refer to any changes that occur in the skin due to reduced or damaged skin barrier function. Examples include increased skin wrinkling, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne, and the like.
  • the "moisturizing skin” may refer to any action that maintains skin moisture or prevents moisture loss.
  • the Danshen extract may be obtained by extracting Danshen with water, C1 to C4 lower alcohol, or a mixture thereof as a solvent. After cutting the Danshen root, it may be extracted by adding 2 to 20 times the weight of the Danshen root with water, alcohol, or a mixture thereof. In addition, the extraction may be performed at 30 ° C to 100 ° C, and may be extracted for 1 to 10 hours.
  • the extraction method may use cold needle extraction, ultrasonic extraction, or reflux cooling extraction method, and the number of extractions may be performed for 1 to 5 times.
  • the saffron flower extract and Danshen root extract may be mixed in a weight ratio of 1:0.01 to 1:10.
  • the saffron flower extract and Danshen root extract are, for example, 1:0.01 to 1:10, 1:0.1 to 1:10, 1:1 to 1:10, 1:5 to 1:10, 1:5 to 1 :8 or 1:7 to 1:9.
  • the mixing ratio of the saffron flower extract and the Danshen root extract is less than the above range, there is a problem in that the synergistic effect of improving skin condition by the saffron flower extract and the Danshen root extract is not sufficiently exhibited.
  • the composition is 0.001% to 80% by weight, for example, 0.01% to 60% by weight, 0.01% to 40% by weight, 0.01% to 30% by weight, based on the total weight of the composition, 0.01% to 20% by weight, 0.01% to 10% by weight, 0.01% to 5% by weight, 0.05% to 60% by weight, 0.05% to 40% by weight, 0.05% to 30% by weight, 0.05% by weight % to 20% by weight, 0.05% to 10% by weight, 0.05% to 5% by weight, 0.1% to 60% by weight, 0.1% to 40% by weight, 0.1% to 30% by weight, 0.1% to 0.1% by weight 20% by weight, 0.1% to 10% by weight, or 0.1% to 5% by weight of a mixture of saffron flower extract and Danshen root extract.
  • the composition according to one aspect may have a synergistic activity of improving skin beauty and wound healing by the mixture of the saffron flower extract and the Danshen root extract.
  • the composition may be a cosmetic composition.
  • the cosmetic composition may have, for example, softening lotion, nutrient lotion, massage cream, nutrient cream, essence, pack, gel, ampoule, or skin-adhesive cosmetic formulation.
  • ingredients included in the cosmetic composition may include ingredients commonly used in cosmetic compositions other than the composition as active ingredients, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and flavors. can include
  • composition may be a composition for external application for skin.
  • the external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof.
  • the external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed.
  • the external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin.
  • Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix
  • the composition may be a pharmaceutical composition.
  • the pharmaceutical composition may additionally include a pharmaceutically acceptable diluent or carrier.
  • the diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and magnesium stearate, talc, or a combination thereof as a lubricant.
  • the carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof.
  • the excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof.
  • the disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, calcium monohydrogen phosphate, or a combination thereof.
  • the binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof.
  • the lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.
  • the pharmaceutical composition may be formulated for oral or parenteral administration.
  • Oral dosage forms may be granules, powders, solutions, tablets, capsules, dry syrups, or combinations thereof.
  • Parenteral dosage forms may be injections.
  • the composition may be a health functional food composition.
  • the health functional food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to conventional methods.
  • the mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment).
  • the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material.
  • beverage compositions may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages.
  • the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • sweetener natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.
  • the health food composition may also contain nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonated beverages. carbonation agent used, or a combination thereof.
  • the health functional food composition may also contain natural fruit juice, fruit juice beverages, fruit flesh for preparing vegetable beverages, or a combination thereof.
  • Another aspect also provides a method of preventing, ameliorating, or treating a condition in a subject comprising treating or administering to a subject in need thereof an effective amount of a composition described above.
  • the condition of the subject may be a skin related condition.
  • administering As used herein, the terms “administering,” “introducing,” and “implanting” are used interchangeably and according to one embodiment into a subject by a method or route that results in at least partial localization of a composition to a desired site. It may mean the arrangement of the composition according to one embodiment of.
  • Administration may be administered by a method known in the art.
  • Administration can be administered directly to a subject by any means, for example, by routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration.
  • routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration.
  • the administration may be administered systemically or locally.
  • the subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat.
  • the subject may be a subject in need of skin condition improvement, for example, skin moisturizing, skin barrier strengthening, skin wound treatment, and skin wrinkle improvement.
  • the administration is 0.1 mg to 1,000 mg per day of the composition according to one embodiment, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg , 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered.
  • the dosage may be variously prescribed depending on factors such as formulation method, administration method, patient's age, weight, sex, medical condition, food, administration time, administration route, excretion rate and response sensitivity, and those skilled in the art can Dosage can be appropriately adjusted in consideration of these factors.
  • the number of administrations can be once a day or two or more times within the range of clinically acceptable side effects, and administration can be done at one or two or more sites, daily or at intervals of 2 to 5 days, for a total of
  • the number of administration days may be administered from 1 day to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period.
  • the same dosage per kg as for humans is used, or the above dosage is converted by the volume ratio (eg, average value) of the organ (heart, etc.) between the target animal and the human.
  • a single dose can be administered.
  • the cosmetic composition according to one aspect contains both a saffron flower extract and a Danshen root extract, and thus has a synergistic effect on wrinkles, elasticity, and moisturizing compared to each single extract, and thus has an excellent effect on improving skin conditions.
  • 1 is a photograph showing the color of a saffron flower extract with or without a decolorization process.
  • Figure 2 is a graph showing the effect of saffron flower extracts on the expression of MMP-1 according to the presence or absence of a crocin compound.
  • Figure 3 is a graph showing the effect of saffron flower extract on collagen production according to the presence or absence of a crocin compound.
  • Figure 4 is a result showing the effect of saffron flower extract on wound healing and skin regeneration according to the presence or absence of a crocin compound.
  • Figure 5 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of MMP-1.
  • Figure 6 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of COL1A1.
  • FIG. 7 is a graph showing the effect of the cosmetic composition according to one embodiment on the production of elastin.
  • FIG. 8 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of fibrillin.
  • 9A and 9B are results showing the skin regeneration promoting effect of the cosmetic composition according to one embodiment.
  • 10A and 10B are graphs showing the skin moisturizing effect of the cosmetic composition according to one embodiment.
  • 11A, 11B and 11C are results showing the skin wrinkle improvement effect according to the formulation of the cosmetic composition according to one embodiment.
  • the saffron flower dried extract prepared in Preparation Example 1 further included a step of decolorizing the colored material using a filtering agent to which 0.5 to 10% by weight of activated carbon was added based on the total weight of the solvent for 10 to 24 hours.
  • a saffron flower water extract was prepared in the same manner as in Preparation Example 1 except for the above.
  • Saffron flower dried extract water extract was prepared in the same manner as in Preparation Example 2, except that the saffron flower water extract prepared in Preparation Examples 3 and 5 was used.
  • Saffron flower ethanol extracts were prepared in the same manner as in Preparation Example 8, except that 25 ° C. ethanol (10%, 25% and 50%) was used.
  • a cosmetic composition was prepared by mixing the saffron flower water extract prepared in Preparation Example 2 and the Danshen root extract prepared in Preparation Example 14 at weight ratios of 1:0.1, 1:0.5, and 1:1.
  • a cosmetic composition in a cream formulation containing the ingredients and contents of Table 1 below was prepared. In this specification, unless otherwise indicated, content is % by weight.
  • a creamy cosmetic composition was prepared in the same manner as in Example 4, except that the components and contents of Table 2 were included.
  • the surface color of the saffron flower extract prepared in Preparation Examples 1 to 13 according to the decolorization process was visually observed.
  • the content of the crocin compound corresponding to the main component of the saffron flower extract was confirmed by HPTLC (High-Performance Thin Layer Chromatography) and shown in Table 3.
  • Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation Example 8 Preparation Example 9 Preparation Example 10 Preparation Example 11 Preparation Example 12 Preparation Example 13 crocin concentration +/- - +/- +/- +/- - - ++++ +/- +/- ++ ++++
  • 1 is a photograph showing the color of a saffron flower extract with or without a decolorization process.
  • the crocin compound which is a chemical substance responsible for the color of saffron, is removed by decolorization.
  • Saffron contains compounds such as crocin, picrocrocin, and safranal as main components. Therefore, it was confirmed whether a difference in anti-aging and anti-wrinkle activity occurs depending on the presence or absence of the crocin compound, which is one of the main components.
  • a human dermal fibroblast cell line Human dermal fibroblast, Hs68
  • UVA 10 J/cm 2 was irradiated or not irradiated through a Biosun UV lamp.
  • Preparation Example 2 and Preparation Example 9 were treated by concentration (1.0% and 2.0%), and further cultured for 48 hours. Then, the expression level of MMP-1 was measured by ELISA.
  • 10 ⁇ g/mL or 100 ⁇ g/mL of Crocin compound was used.
  • Figure 2 is a graph showing the effect of saffron flower extracts on the expression of MMP-1 according to the presence or absence of a crocin compound.
  • a human dermal fibroblast cell line Human dermal fibroblast, Hs68
  • Preparation Example 2 and Preparation Example 9 were treated by concentration (1.0% and 2.0%), and further cultured for 24 hours.
  • the culture supernatant was stored at -80 ° C, and the amount of collagen expression was measured by ELISA.
  • 10 ⁇ g/mL of Crocin compound was used.
  • Figure 3 is a graph showing the effect of saffron flower extract on collagen production according to the presence or absence of a crocin compound.
  • Preparation Example 2 and Preparation Example 9 was confirmed to increase the expression of collagen in a concentration-dependent manner.
  • the saffron flower extract according to one embodiment may exhibit an effect of improving skin elasticity regardless of the presence or absence of the crocin compound.
  • a human dermal fibroblast cell line (Hs68) was cultured in an incubator at 37° C. and 5% CO 2 to reach 100% confluency. Thereafter, using a pipette tip, randomly scrape across the center of the well to make a wound, and then replace the above Preparation Example 2 and Preparation Example 9 with a medium containing 1.0%, respectively, for an additional 24 hours. cultured. Then, cell migration of the fibroblasts was observed using an electron microscope. As a positive control group, 10 ⁇ g/mL of Crocin compound was used.
  • Figure 4 is a result showing the effect of saffron flower extract on wound healing and skin regeneration according to the presence or absence of a crocin compound.
  • the saffron flower extract according to one embodiment can not only treat skin wounds by promoting cell migration in aged fibroblasts, but also exhibit skin regeneration effects, even though it does not contain a crocin compound.
  • the saffron flower extract has a skin condition improvement effect regardless of the presence or absence of the crocin compound. Therefore, in the following, it was confirmed whether the mixture of the saffron flower extract and the Danshen root extract has synergistic activity of anti-aging and anti-wrinkle compared to each single extract.
  • human dermal fibroblast cell line Human dermal fibroblast, Hs68 was divided into 3x10 5 in a 6-well plate, and then cultured for 24 hours in an incubator at 37°C and 5% CO 2 conditions.
  • Figure 5 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of MMP-1.
  • Figure 6 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of COL1A1.
  • the cosmetic composition according to one aspect reduces the expression of MMP-1 increased by ultraviolet rays and increases the expression of COL1A1 by including a saffron flower extract and a Danshen root extract, thereby having excellent anti-aging and anti-wrinkle activity.
  • the human dermal fibroblast cell line Human dermal fibroblast, Hs68
  • FIG. 7 is a graph showing the effect of the cosmetic composition according to one embodiment on the production of elastin.
  • FIG. 8 is a graph showing the effect of the cosmetic composition according to one embodiment on the expression of fibrillin.
  • the cosmetic composition according to one aspect includes a saffron flower extract and a Danshen root extract, the expression of elastin and fibrillin is significantly increased compared to each single extract, and thus, a synergistic effect of improving skin elasticity can be exhibited.
  • the human dermal fibroblast cell line Human dermal fibroblast, Hs68
  • Hs68 human dermal fibroblast, Hs68
  • the medium was replaced with a medium containing 1.0% of Examples 1 to 3, respectively, and further cultured for 24 hours.
  • cell migration of the fibroblasts was observed using an electron microscope.
  • bEGF 10 ng/ml was used as a positive control group.
  • 9A and 9B are results showing the skin regeneration promoting effect of the cosmetic composition according to one embodiment.
  • the cosmetic composition according to one aspect may have excellent effects on wound healing and skin regeneration by including the saffron flower extract and the Danshen root extract.
  • Example 4 Comparative Example 1 twice a day for 4 weeks on the left/right side of the face. Then, the test site was washed with soap and waited in a constant temperature and humidity room (22 ⁇ 2 °C, 40 ⁇ 60% RH) for 10 minutes, and then the skin moisture level (0 weeks) was measured and skin wrinkles (0 weeks) were photographed. . After 2 weeks and 4 weeks of product use, skin moisture level and skin wrinkles were measured in the same way, and the average of the three measured values in each week was calculated and compared with the measured values in the 0th week.
  • 10A and 10B are graphs showing the skin moisturizing effect of the cosmetic composition according to one embodiment.
  • 11A, 11B and 11C are results showing the skin wrinkle improvement effect of the cosmetic composition according to one embodiment.
  • Example 4 As a result, as shown in FIGS. 10A and 10B, it was confirmed that the skin moisture level after 4 weeks of use was 24.3% and 5.0% in Example 4 and Comparative Example 1, respectively, and increased compared to before use. In particular, in Example 4, it was confirmed that the skin moisture level was significantly increased compared to Comparative Example 1.
  • Example 4 and Comparative Example 1 decreased skin indentation as the use time increased.
  • Example 4 it was confirmed that the rate of change in skin subsidence at the 4th week of use was significantly reduced compared to Comparative Example 1.
  • the cosmetic composition according to one aspect has an excellent effect on skin moisturizing and wrinkle improvement.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Public Health (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen. Un mélange de l'extrait de fleur de safran et de l'extrait de danshen présente un effet synergique sur les rides, l'élasticité et l'hydratation, et présente ainsi un excellent effet sur l'amélioration de la beauté de la peau.
PCT/KR2023/001653 2022-02-07 2023-02-06 Composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen WO2023149766A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2022-0015771 2022-02-07
KR1020220015771A KR20230119520A (ko) 2022-02-07 2022-02-07 샤프란 꽃 추출물 및 단삼 뿌리 추출물을 포함하는 화장료 조성물

Publications (1)

Publication Number Publication Date
WO2023149766A1 true WO2023149766A1 (fr) 2023-08-10

Family

ID=87552698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2023/001653 WO2023149766A1 (fr) 2022-02-07 2023-02-06 Composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen

Country Status (2)

Country Link
KR (1) KR20230119520A (fr)
WO (1) WO2023149766A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101361516A (zh) * 2008-09-18 2009-02-11 赵人洁 一种中药美容保健茶
KR101742866B1 (ko) * 2015-04-10 2017-06-15 서인경 피부 탄력 및 주름 개선용 화장료 조성물
CN110538099A (zh) * 2018-05-29 2019-12-06 范丽荣 一种植物精粹凝润紧致焕肤养颜面膜
CN110859792A (zh) * 2019-11-11 2020-03-06 李利 一种具有细胞修复功效面膜及其制备方法
CN112587657A (zh) * 2020-12-29 2021-04-02 山东丽达生物科技集团有限公司 促进皮肤健康滋润嫩红的中药生物制剂及其制备方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101361516A (zh) * 2008-09-18 2009-02-11 赵人洁 一种中药美容保健茶
KR101742866B1 (ko) * 2015-04-10 2017-06-15 서인경 피부 탄력 및 주름 개선용 화장료 조성물
CN110538099A (zh) * 2018-05-29 2019-12-06 范丽荣 一种植物精粹凝润紧致焕肤养颜面膜
CN110859792A (zh) * 2019-11-11 2020-03-06 李利 一种具有细胞修复功效面膜及其制备方法
CN112587657A (zh) * 2020-12-29 2021-04-02 山东丽达生物科技集团有限公司 促进皮肤健康滋润嫩红的中药生物制剂及其制备方法

Also Published As

Publication number Publication date
KR20230119520A (ko) 2023-08-16

Similar Documents

Publication Publication Date Title
WO2020060060A1 (fr) Composition cosmétique comprenant un extrait de racine adventive de centella asiatica en tant que principe actif pour le blanchiment de la peau et la réduction des rides
WO2018174453A1 (fr) Composition contenant un extrait de cirsium ja p onicum en tant que principe actif pour stimuler la mélanogenèse
WO2019066121A1 (fr) Composition comprenant des vésicules extracellulaires dérivées de plantes
WO2023177188A1 (fr) Composition cosmétique ayant des effets anti-inflammatoires et de barrière, comprenant un extrait de centella asiatica à l'état frais obtenu par extraction de sucre par diffusion, et son utilisation
WO2019143055A1 (fr) Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés
KR102578892B1 (ko) 식물 추출물을 바실러스 균주로 발효한 발효물을 포함하는 화장료 조성물
KR102484422B1 (ko) 사막 선인장 추출물 및 락토바실러스 배양액을 포함하는 화장료 조성물
WO2022145960A1 (fr) Souche de weissella cibaria et son utilisation
WO2023149766A1 (fr) Composition cosmétique comprenant un extrait de fleur de safran et un extrait de danshen
KR102367436B1 (ko) 어린탱자, 어린녹차 및 비터오렌지꽃의 혼합 발효물을 포함하는 조성물 및 그의 피부 상태 개선 용도
KR102176883B1 (ko) 복사나무 수지를 함유하는 피부 재생, 주름 개선, 또는 상처 치유용 조성물
KR102321536B1 (ko) 코리네박테리움 아미콜라툼 균주 및 그의 피부 상태 개선 용도
KR102334454B1 (ko) 코리네박테리움 투베르쿨로스테아리쿰 균주 및 그의 피부 상태 개선 용도
KR102586125B1 (ko) 복합 식물 추출물을 함유하는 나노리포좀을 유효성분으로 포함하는 항산화, 항염 및 피부 항노화 조성물
WO2020111540A1 (fr) Composition pharmaceutique pour la prévention ou le traitement du vieillissement ou de maladies associées au vieillissement, comprenant un complexe d'anthocyanine-polysaccharide en tant que principe actif
WO2023224274A1 (fr) Composition cosmétique comprenant un extrait supercritique de cellules de la souche fusidium coccineum
WO2022114784A1 (fr) Composition comprenant un produit de fermentation d'une huile de cactus et son utilisation pour améliorer l'état de la peau
KR102675543B1 (ko) 스노우베리 발효물을 포함하는 조성물 및 그의 피부 상태 개선 용도
WO2023090708A1 (fr) Composition comprenant du sirop de grains d'avoine ou un extrait de celui-ci pour l'hydratation de la peau, la réduction des rides et l'antioxydation
KR20200142376A (ko) 코코넛 추출물을 함유하는 피부 미백, 항주름, 또는 보습용 조성물
KR102515074B1 (ko) 차즈기 발효물을 유효성분으로 포함하는 화장료 조성물
KR102334456B1 (ko) 판토에아 아나나티스 균주 및 그의 피부 상태 개선 용도
WO2022114783A1 (fr) Composition contenant un produit fermenté de miel de cactus et son utilisation pour améliorer l'état de la peau
KR102334457B1 (ko) 마이크로박테리움 올레이보란스 균주 및 그의 피부 상태 개선 용도
WO2023018077A1 (fr) Souche de microccocus cohnii et son utilisation pour améliorer l'état de la peau

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23749999

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE