WO2022104862A1 - 一种促进工业大麻生根的方法 - Google Patents
一种促进工业大麻生根的方法 Download PDFInfo
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- WO2022104862A1 WO2022104862A1 PCT/CN2020/131641 CN2020131641W WO2022104862A1 WO 2022104862 A1 WO2022104862 A1 WO 2022104862A1 CN 2020131641 W CN2020131641 W CN 2020131641W WO 2022104862 A1 WO2022104862 A1 WO 2022104862A1
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- cutting
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- industrial hemp
- cuttings
- rock wool
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 244000025254 Cannabis sativa Species 0.000 title claims abstract description 28
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 title claims abstract description 28
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 title claims abstract description 28
- 235000009120 camo Nutrition 0.000 title claims abstract description 28
- 235000005607 chanvre indien Nutrition 0.000 title claims abstract description 28
- 239000011487 hemp Substances 0.000 title claims abstract description 28
- 230000001737 promoting effect Effects 0.000 title claims abstract description 11
- 238000005520 cutting process Methods 0.000 claims abstract description 94
- 239000011490 mineral wool Substances 0.000 claims description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000003337 fertilizer Substances 0.000 claims description 20
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 238000012423 maintenance Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 5
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- 230000000717 retained effect Effects 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 2
- NGLMYMJASOJOJY-UHFFFAOYSA-O azanium;calcium;nitrate Chemical compound [NH4+].[Ca].[O-][N+]([O-])=O NGLMYMJASOJOJY-UHFFFAOYSA-O 0.000 claims description 2
- 239000012452 mother liquor Substances 0.000 claims description 2
- 239000012224 working solution Substances 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000011282 treatment Methods 0.000 description 33
- 241000218236 Cannabis Species 0.000 description 5
- 230000002354 daily effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000013138 pruning Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 206010061217 Infestation Diseases 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
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- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000001228 spectrum Methods 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
Definitions
- the invention belongs to the technical field of industrial hemp cultivation, and in particular relates to a method for promoting the rooting of industrial hemp.
- Medicinal industrial hemp is a plant of the genus Moraceae industrial hemp, which has high comprehensive utilization value and is widely used in fiber raw materials, functional food production, new materials, and medical and health care fields.
- the propagation methods of medicinal industrial hemp mainly include seed propagation and cutting propagation. Cutting propagation can make the seedlings uniform and fully maintain the characteristics of the original variety, so it has been used more and more in recent years.
- the traditional cutting method is generally carried out outdoors using natural light, and some cuttings cultivated indoors mainly use fluorescent lamps as the light source.
- Fluorescent light is a full-spectrum light source, and the use of this light source is slower for roots and longer for seedling emergence.
- the purpose of the present invention is to provide a method for promoting the rooting of industrial hemp.
- a method for promoting the rooting of industrial hemp which includes the use of blue light with a wavelength of 410nm-460nm as an irradiation light source during indoor cutting propagation.
- the wavelength of the blue light is 410 nm.
- the environmental conditions during cutting propagation are: temperature 25°C-27°C, light intensity 61-80 umol/m2/s, and relative air humidity 70-80%.
- the cutting substrate is immersed in a complete nutrient solution with an EC of 0.4-0.5 ms/cm and a pH of 5.6-6.0.
- the relative water content of the cutting substrate is controlled to be between 75-85%.
- the cutting substrate is rock wool.
- the formula of the complete nutrient solution A fertilizer (calcium ammonium nitrate 22.72g/L, KNO 3 12g/L, EDTA-Fe 0.96g/L), B fertilizer (KH 2 PO 4 11.738g/L, MgSO 4 7H 2 O 10.138g/L, MgCl 2 6H 2 O 0.666g/L), C fertilizer (MnSO 4 H 2 O 113.2mg/L, H 3 BO 3 50mg/L, ZnSO 4 7H 2 O 38mg/L, CuSO 4 ⁇ 5H 2 O 16.6mg/L, Na 2 MoO 4 4.6mg/L, CoCl 2 ⁇ 6H 2 O 8.6mg/L, NiCl 2 ⁇ 6H 2 O 8.6mg/L) configured as concentrated mother liquor , and then diluted to a working solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0.
- the daily maintenance of the cutting propagation is: spraying foliar fertilizer immediately after the cutting is completed, and the foliar fertilizer is a mixed solution of 0.1% urea and 0.1% potassium dihydrogen phosphate, then sprayed twice every day, and every 2 -3 days, the rock wool blocks on the cuttings need to be dipped in the full nutrient solution once.
- the present invention also provides an indoor cutting propagation method of industrial hemp, including the following:
- Configure rooting agent Dilute 5% indine ⁇ naphthalene acetic acid by 200 times to prepare rooting liquid, soak the base of the trimmed cuttings in the rooting liquid for 1 min, and then insert the treated cuttings into the rock wool block , the insertion depth is about 2.5cm, and the blue light is turned on;
- the foliar fertilizer is a mixture of 0.1% urea and 0.1% potassium dihydrogen phosphate, and then spray once every morning and afternoon, and every 2-3 days, The cuttings need to be dipped in the full nutrient solution once.
- the cutting substrate is rock wool.
- the cutting propagation environment conditions are: the indoor temperature of the nursery room is 25-27°C, the light intensity is 61-80umol/m2/s, the relative air humidity is 70-80%; the humidity in the nursery box is 80-90%.
- the base of the cutting is immersed in RO water immediately after being cut from the mother plant.
- the beneficial effects of the invention are as follows: using the method of the invention can promote rooting, early emergence of seedlings, shorten the production cycle, and the root system has stronger growth potential, which is conducive to the cultivation of strong seedlings in the later stage.
- the present invention adopts blue light of a single wavelength for irradiation, which is easy to operate and can reduce the production cost.
- Fig. 1 is a graph showing the variation of rooting rate of industrial hemp with time in different lamps of Example 1;
- Fig. 2 is a graph showing the variation of rooting rate of industrial hemp over time with different lamps of Example 2;
- Fig. 3 is embodiment 3 room temperature 21-23 °C-different lamp treatment industrial hemp rooting rate with time curve diagram;
- Fig. 4 is a graph showing the variation of rooting rate of industrial hemp with time at room temperature 23-25 °C of embodiment 3-different lamps;
- FIG. 5 is a graph showing the variation of rooting rate of industrial hemp with time in Example 3 at room temperature of 25-27° C.-different lamps.
- the three-piece seedling box is used for cleaning and disinfection, and the agricultural rock wool blocks (3.6*3.6*4cm in size) are immersed in the complete nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0, and the rock wool is controlled by weighing method.
- the foliar fertilizer was sprayed immediately after the cutting was completed.
- the experiment was carried out to the 8th day, and the rooting rate of each treatment was counted. On the 12th day, the rooting rate, the number of rooting, and the average root length of each treatment were counted. The comparison results are shown in Table 1 and Figure 1.
- the cutting propagation experiment was carried out in an artificial light indoor environment.
- the types of lamps used are T5 lamps (CK treatment), blue light 410nm, blue light 430nm, blue light 450nm, and blue light 460nm lamps.
- the specific test steps are as follows:
- the three-piece seedling box is used for cleaning and disinfection, and the agricultural rock wool blocks (3.6*3.6*4cm in size) are immersed in the complete nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0, and the rock wool is controlled by weighing method.
- the foliar fertilizer was sprayed immediately after the cutting was completed.
- the experiment was carried out to the 8th day, and the rooting rate of each treatment was counted. On the 12th day, the rooting rate, the number of rooting, and the average root length of each treatment were counted. The comparison results are shown in Table 2 and Figure 2.
- the rooting speed of each wavelength of blue light of the present embodiment 2 is significantly faster than that of the control group (T5), and the rooting time is 1 day earlier than the control group, and the emergence time is except the blue light 460nm treatment, 1d earlier than the control group.
- the root lengths of the blue light 410nm, 430nm, 450nm and 460nm treatments were 2.09 times, 1.82 times, 2.15 times and 1.97 times that of the T5 treatment, respectively, and the rooting numbers were 2.02 times, 1.61 times, 1.96 times and 1.86 times that of the T5 treatment, respectively. It can be seen that all wavelengths of blue light can promote cannabis rooting.
- the three-piece seedling box is used for cleaning and disinfection, and the agricultural rock wool blocks (3.6*3.6*4cm in size) are immersed in the complete nutrient solution with EC of 0.4-0.5ms/cm and PH of 5.6-6.0, and the rock wool is controlled by weighing method.
- Environmental condition control light intensity is 40-60umol/m2/s, relative air humidity is 70-80%; humidity in the seedling box is 80-90%.
- the foliar fertilizer was sprayed immediately after the cutting was completed.
- the test was carried out to the 6th day, and the rooting rate of each treatment was counted. After the 13th day, the rooting rate, the number of rooting, and the average root length of each treatment were counted. The comparison results are shown in Table 3, Figure 3, Figure 4, and Figure 5.
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Hydroponics (AREA)
- Cultivation Of Plants (AREA)
Abstract
一种促进工业大麻生根的方法,包括在室内扦插繁殖时,照射光源采用波长为410nm-460nm的蓝光。采用该方法可促进生根,提早出苗,缩短生产周期,且根系生长势更强,有利于后期培育壮苗,并且该方法采用单一波长的蓝光进行照射,易操作,可降低生产成本。
Description
本发明属于工业大麻种植技术领域,具体涉及一种促进工业大麻生根的方法。
药用工业大麻是桑科工业大麻属植物,具有较高的综合利用价值,广泛运用于纤维原料、功能食品生产、新材料以及医药保健领域。
药用工业大麻繁殖方法主要有种子繁殖和扦插繁殖。扦插繁殖可以使种苗整齐一致,充分保持原有品种的特性,因此近年来越来越多被采用。
传统扦插方法一般是在室外利用自然光进行,部分室内栽培的扦插主要是采用荧光灯作为光源。荧光灯属全光谱光源,采用这种光源发根较慢,出苗时间长。
发明内容
有鉴于此,本发明的目的是提供一种促进工业大麻生根的方法。
本发明采取的具体技术方案是:
一种促进工业大麻生根的方法,包括在室内扦插繁殖时,照射光源采用波长为410nm-460nm的蓝光。
优选地,所述蓝光的波长为410nm。
优选地,扦插繁殖时的环境条件为:温度25℃-27℃,光强61-80umol/㎡/s,空气相对湿度为70-80%。
优选地,所述扦插繁殖时,将扦插基质浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中。
优选地,扦插繁殖时,控制扦插基质相对含水量在75-85%之间。
优选地,所述扦插基质为岩棉。
优选地,所述全营养液的配方:A肥(硝酸铵钙22.72g/L,KNO
3 12g/L,EDTA-Fe 0.96g/L)、B肥(KH
2PO
4 11.738g/L,MgSO
4·7H
2O 10.138g/L,MgCl
2·6H
2O 0.666g/L)、C肥(MnSO
4·H
2O 113.2mg/L,H
3BO
3 50mg/L,ZnSO
4·7H
2O 38mg/L,CuSO
4·5H
2O 16.6mg/L,Na
2MoO
4 4.6mg/L,CoCl
2·6H
2O 8.6mg/L,NiCl
2·6H
2O 8.6mg/L)配置成浓缩母液,再稀释成EC为0.4-0.5ms/cm、PH为5.6-6.0的工作液。
优选地,所述扦插繁殖的日常养护为:扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天喷施两次,且每隔2-3天,扦插苗上的岩棉块需蘸取全营养液一次。
相应地,本发明还提供了一种工业大麻室内扦插繁殖方法,包括如下:
(1)扦插前准备工作:育苗盒用清洗消毒备用,将扦插基质浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中,并用称重法控制扦插基质相对含水量保持在75-85%,将扦插基质摆放在育苗盒内备用,育苗盒内设有波长为410nm-460nm的蓝光灯。
(2)选取插穗:在长势健壮、无病虫害侵染的母株中选取插穗,并对其进行修剪:长度18cm左右,基部粗度2.0-3.0mm,留取一顶芽二腋芽并保留腋芽对应叶片;
(3)配置生根剂:将5%吲叮·萘乙酸稀释200倍配置成生根液,将修剪好的插穗基部浸泡在生根液中,时间1min,再将处理好的插穗插至岩棉块中,插入深度约2.5cm,打开蓝光灯;
(4)日常养护:扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天上下午各喷施一次,且每隔2-3天,扦插苗需蘸取全营养液一次。
优选地,所述扦插基质为岩棉。
优选地,扦插繁殖环境条件为:育苗室室内温度25-27℃,光强61-80umol/㎡/s,空气相对湿度70-80%;育苗盒内湿度为80-90%。
优选地,所述插穗从母株上剪下后立即将其基部浸泡在RO水中。
本发明的有益效果是:采用本发明的方法可促进生根,提早出苗,缩短生产周期,且根系生长势更强,有利于后期培育壮苗。并且本发明采用单一波长的蓝光进行照射,易操作,且可降低生产成本。
图1为实施例1不同灯处理工业大麻生根率随时间变化曲线图;
图2为实施例2不同灯处理工业大麻生根率随时间变化曲线图;
图3为实施例3室温21-23℃-不同灯处理工业大麻生根率随时间变化曲线图;
图4为实施例3室温23-25℃-不同灯处理工业大麻生根率随时间变化曲线图;
图5为实施例3室温25-27℃-不同灯处理工业大麻生根率随时间变化曲线图。
下面结合实施例对本发明作进一步地详细说明,但本发明的实施方式不限于此,在不脱离本发明上述技术思想情况下,根据本领域普通技术知识和惯用手段,做出各种替换和变更,均应包括在本发明的范围内。
实施例1
2020年8月7日在人工光室内环境下进行扦插繁殖试验。所用灯类型分别为T5灯管(CK处理)、蓝光410nm、蓝光450nm灯,具体试验步骤如下:
(1)扦插前准备工作。育苗盒三件套用清洗消毒备用,将农用岩棉块(规格3.6*3.6*4cm)浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中,并用称重法控制岩棉相对含水量保持在75-85%{岩棉相对含水量=(岩棉吸水时重量-岩棉重量)/(岩棉充分吸水时重量-岩棉重量)*100%},将岩棉摆放在育苗盒内备用,每盒15块岩棉,即每种灯处理扦插5株,重复3次。
(2)选取插穗。在长势健壮、无病虫害侵染的母株中选取插穗,经修剪 后的插穗要求:长度18cm左右,基部粗度2.0-3.0mm,留取一顶芽二腋芽并保留腋芽对应叶片。插穗从母株上剪下后需立即将基部浸泡在RO水中。
(3)配置生根剂,5%吲叮·萘乙酸(国光·根盼)稀释200倍配置成生根液,将修剪好的插穗基部浸泡在生根液中,时间1min,再将处理好的插穗插至岩棉块中,插入深度约2.5cm。
(4)环境条件控制:育苗室室内温度23-25℃,光强40-60umol/㎡/s,空气相对湿度70-80%;育苗盒内湿度80-90%为宜。
(5)日常养护。扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天上下午各喷施一次。每隔2-3天,扦插苗上的岩棉块需蘸取全营养液(EC=0.4-0.5ms/cm,PH=5.6-6.0)一次。试验进行至第8d,统计各个处理的生根率,进行至第12d,统计各个处理的生根率、生根数量、平均根长,对比结果见表1、图1。
表1 不同灯处理对工业大麻插穗生根的影响
注:统计时间第12d。
由图1和表1可知,采用本实施例1的蓝光410mm处理工业大麻生根速度显著快于对照组(T5),生根时间及出苗时间均比对照组提前1天,且平均根长比对照组长1.06cm,生根数量比对照组多17.93条。蓝光450mm处理生根速度与T5处理相比没有显著差异,但根长较长、生根数量较多。
实施例2
2020年8月8日在人工光室内环境下进行扦插繁殖试验。所用灯类型分别为T5灯管(CK处理)、蓝光410nm、蓝光430nm、蓝光450nm、蓝光460nm灯, 具体试验步骤如下:
(6)扦插前准备工作。育苗盒三件套用清洗消毒备用,将农用岩棉块(规格3.6*3.6*4cm)浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中,并用称重法控制岩棉相对含水量保持在75-85%{岩棉相对含水量=(岩棉吸水时重量-岩棉重量)/(岩棉充分吸水时重量-岩棉重量)*100%},将岩棉摆放在育苗盒内备用,每盒15块岩棉,即每种灯处理扦插5株,重复3次。
(7)选取插穗。在长势健壮、无病虫害侵染的母株中选取插穗,经修剪后的插穗要求:长度18cm左右,基部粗度2.0-3.0mm,留取一顶芽二腋芽并保留腋芽对应叶片。插穗从母株上剪下后需立即将基部浸泡在RO水中。
(8)配置生根剂,5%吲叮·萘乙酸(国光·根盼)稀释200倍配置成生根液,将修剪好的插穗基部浸泡在生根液中,时间1min,再将处理好的插穗插至岩棉块中,插入深度约2.5cm。
(9)环境条件控制:育苗室室内温度23-25℃,光强40-60umol/㎡/s,空气相对湿度70-80%;育苗盒内湿度80-90%为宜。
(10)日常养护。扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天上下午各喷施一次。每隔2-3天,扦插苗上的岩棉块需蘸取全营养液(EC=0.4-0.5ms/cm,PH=5.6-6.0)一次。试验进行至第8d,统计各个处理的生根率,进行至第12d,统计各个处理的生根率、生根数量、平均根长,对比结果见表2、图2。
表2 不同灯处理对工业大麻插穗生根的影响
注:统计时间第12d。
由图2和表2可知,采用本实施例2的蓝光各个波长处理的生根速度均显著快于对照组(T5),开始生根时间均比对照组提前1天,出苗时间除蓝光460nm处理外,均比对照组提前1d。蓝光410nm、430nm、450nm、460nm处理的根长分别是T5处理的2.09倍、1.82倍、2.15倍、1.97倍,生根数量分别是T5处理的2.02倍、1.61倍、1.96倍、1.86倍。由此可见,蓝光各个波长处理均能促进大麻生根。
实施例3
2020年9月8日在人工光室内环境下进行扦插繁殖试验。所用灯类型分别为T5灯管(CK处理)、蓝光410nm、蓝光450nm,分别在室温21-23℃、23-25℃、25-27℃的实验室内进行试验,每个室温处理内做3个灯处理,每个处理5株,重复3次,具体试验步骤如下:
(11)扦插前准备工作。育苗盒三件套用清洗消毒备用,将农用岩棉块(规格3.6*3.6*4cm)浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中,并用称重法控制岩棉相对含水量保持在75-85%{岩棉相对含水量=(岩棉吸水时重量-岩棉重量)/(岩棉充分吸水时重量-岩棉重量)*100%},将岩棉摆放在育苗盒内备用。
(12)选取插穗。在长势健壮、无病虫害侵染的母株中选取插穗,经修剪后的插穗要求:长度18cm左右,基部粗度2.0-3.0mm,留取一顶芽二腋芽并保留腋芽对应叶片。插穗从母株上剪下后需立即将基部浸泡在RO水中。
(13)配置生根剂,5%吲叮·萘乙酸(国光·根盼)稀释200倍配置成生根液,将修剪好的插穗基部浸泡在生根液中,时间1min,再将处理好的插穗插至岩棉块中,插入深度约2.5cm。
(14)环境条件控制:光强40-60umol/㎡/s,空气相对湿度70-80%;育苗盒内湿度80-90%为宜。
(15)日常养护。扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天上下午各喷施一次。每隔2-3天,扦插苗上的岩棉块需蘸取全营养液(EC=0.4-0.5ms/cm,PH=5.6-6.0)一次。试验进行至第6d,统计各个处理的生根率,进行至第13d,统计各个处理的生根率、生根数量、平均根长,对比结果见表3、图3、图4、图5。
表3 不同灯不同室温处理对大麻插穗生根的影响
注:统计时间第13d。
由图3、图4、图5、表3可知,在室温21-27℃内,蓝光410nm、蓝光450nm处理的大麻插穗生根速率均显著快于T5灯处理,且大麻插穗出苗时间均比T5灯处理早1天以上,其中室内25-27℃处理下出苗时间最早,其次为室温23-25℃处理,最晚的是室温21-23℃处理;不论何种室温处理,蓝光处理下大麻插穗的根长及生根数量均显著高于T5处理。综上所述,蓝光410nm、蓝光450nm处理均优于T5处理,且室温为25-27℃处理最佳。
尽管已经对上述各实施例进行了描述,但本领域内的技术人员一旦得知了基本创造性概念,则可对这些实施例做出另外的变更和修改,所以以上所述仅为本发明的实施例,并非因此限制本发明的专利保护范围,凡是利用本发明说明书及附图内容所作的等效结构或等效流程变换,或直接或间接运用在其他相关的技术领域,均同理包括在本发明的专利保护范围之内。
Claims (10)
- 一种促进工业大麻生根的方法,其特征在于,包括在室内扦插繁殖时,照射光源采用波长为410nm-460nm的蓝光。
- 根据权利要求1所述的促进工业大麻生根的方法,其特征在于,所述蓝光的波长为410nm。
- 根据权利要求1或2所述的促进工业大麻生根的方法,其特征在于,扦插繁殖时的环境条件为:温度21-28℃,光强30-90umol/㎡/s,空气相对湿度60-80%,优选:温度25℃-27℃,光强61-80umol/㎡/s,空气相对湿度为70-80%。
- 根据权利要求1或2所述的促进工业大麻生根的方法,其特征在于,所述扦插繁殖时,将扦插基质浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中。
- 根据权利要求4所述的促进工业大麻生根的方法,其特征在于,还包括以下特征的任一项或多项:(1)扦插繁殖时,控制扦插基质相对含水量在75-85%之间;(2)所述扦插基质为岩棉;(3)所述全营养液的配方:A肥(硝酸铵钙22.72g/L,KNO 312g/L,EDTA-Fe 0.96g/L)、B肥(KH 2PO 411.738g/L,MgSO 4·7H 2O 10.138g/L,MgCl 2·6H 2O 0.666g/L)、C肥(MnSO 4·H 2O 113.2mg/L,H 3BO 350mg/L,ZnSO 4·7H 2O 38mg/L,CuSO 4·5H 2O 16.6mg/L,Na 2MoO 44.6mg/L,CoCl 2·6H 2O 8.6mg/L,NiCl 2·6H 2O 8.6mg/L)配置成浓缩母液,再稀释成EC为0.4-0.5ms/cm、PH为5.6-6.0的工作液。
- 根据权利要求5所述的促进工业大麻生根的方法,其特征在于,所述扦插繁殖的日常养护为:扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天喷施两次,且每隔2-3天,扦插苗上的岩棉块需蘸取全营养液一次。
- 一种工业大麻室内扦插繁殖方法,其特征在于,包括如下:(1)扦插前准备工作:育苗盒用清洗消毒备用,将扦插基质浸泡在EC为0.4-0.5ms/cm、PH为5.6-6.0的全营养液中,并用称重法控制扦插基质相 对含水量保持在75-85%,将扦插基质摆放在育苗盒内备用,育苗盒内设有波长为410nm-460nm的蓝光灯;(2)选取插穗:在长势健壮、无病虫害侵染的母株中选取插穗,并对其进行修剪:长度18cm左右,基部粗度2.0-3.0mm,留取一顶芽二腋芽并保留腋芽对应叶片;(3)配置生根剂:将5%吲叮·萘乙酸稀释200倍配置成生根液,将修剪好的插穗基部浸泡在生根液中,时间1min,再将处理好的插穗插至岩棉块中,插入深度约2.5cm,打开蓝光灯;(4)日常养护:扦插完成后立即喷施叶面肥,叶面肥为0.1%尿素与0.1%磷酸二氢钾混合液,此后每天上下午各喷施一次,且每隔2-3天,扦插苗上的岩棉块需蘸取全营养液一次。
- 根据权利要求7所述的工业大麻室内扦插繁殖方法,其特征在于,所述扦插基质为岩棉。
- 根据权利要求7所述的工业大麻室内扦插繁殖方法,其特征在于,扦插繁殖环境条件为:育苗室室内温度25-27℃,光强61-80umol/㎡/s,空气相对湿度70-80%;育苗盒内湿度为80-90%。
- 根据权利要求7所述的工业大麻室内扦插繁殖方法,其特征在于,所述插穗从母株上剪下后立即将其基部浸泡在RO水中。
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