WO2022047862A1 - Composition de médecine chinoise traditionnelle pour la pousse des cheveux, son procédé de préparation et son utilisation - Google Patents

Composition de médecine chinoise traditionnelle pour la pousse des cheveux, son procédé de préparation et son utilisation Download PDF

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WO2022047862A1
WO2022047862A1 PCT/CN2020/117709 CN2020117709W WO2022047862A1 WO 2022047862 A1 WO2022047862 A1 WO 2022047862A1 CN 2020117709 W CN2020117709 W CN 2020117709W WO 2022047862 A1 WO2022047862 A1 WO 2022047862A1
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extract
extraction
spray
chinese medicine
hair
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PCT/CN2020/117709
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Chinese (zh)
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周厚成
胡昌江
段鑫
吴雪梅
唐翠
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四川绿色本草科技发展有限公司
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/483—Gleditsia (locust)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A61K36/638—Ligustrum, e.g. Chinese privet
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
    • A61K36/704—Polygonum, e.g. knotweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Definitions

  • the invention relates to a traditional Chinese medicine composition for hair growth and its preparation method and application, in particular to a traditional Chinese medicine composition capable of activating scalp hair follicles for hair growth, its preparation method and application, and belongs to the field of traditional Chinese medicine preparations.
  • Hair loss is one of the common clinical skin diseases, usually manifested as abnormal or excessive hair loss, such as seborrheic alopecia, alopecia areata, etc.
  • the common causes are endocrine disorders, mental trauma, vascular dysfunction and genetic factors.
  • Chinese medicine has a long history of treating hair loss.
  • hair is closely related to the liver and kidney.
  • the kidney stores essence and the liver controls blood.
  • the ability to synthesize melanin is weakened, resulting in gray hair; in the other case, hair follicles atrophy or die, resulting in hair loss.
  • the liver and kidneys are strong, and the head is developed, the hair will be thick and black.
  • existing research also shows that promoting hair follicle growth is the key to preventing hair loss.
  • the existing patent document CN102973727A (a kind of hair follicle nutrient activation solution and preparation method thereof, 2013.03.20) discloses the use of red ginseng, angelica, arborvitae, chuanxiong, astragalus, safflower, rhubarb, saponin, raw ground, licorice , Black sesame, Tougucao, Shouwu, mulberry, gentian, chrysanthemum, wolfberry, the control test shows that the hair follicle nutrient activation solution can increase the activation rate of hair follicles by more than 70%, And after a small-scale trial, the effect of growth and development is good.
  • CN103768275A (a kind of traditional Chinese medicine hair growth liquid for the treatment of seborrheic alopecia, 2014.05.07) discloses using myrrh, Candelilla, Artemisia argyi, Artemisia annua, Didingcao, Polygonum multiflorum, dog chi, psoralen , Rhizoma Drynariae, Rehmannia glutinosa, Jin Yingzi, Astragalus, the traditional Chinese medicine generating liquid is sprayed on the surface of hair loss through the traditional Chinese medicine generating liquid, which has the functions of drying dampness and deworming, killing poison, killing mold, antifungal, and anti-inflammatory, and has the functions of nourishing blood and nourishing blood.
  • Yin filling essence and marrow, invigorating kidney and strengthening yang, dispersing blood and removing blood stasis, reducing swelling and promoting muscle, tonifying blood vessels, eliminating chronic inflammation of hair follicles and scalp, killing lipophilic fungi and head mites, removing pollutants in hair follicles, inhibiting male and female Excessive secretion of hormones activates the growth of hair follicles. After two to four months of medication, hair can be regenerated.
  • the object of the present invention is to provide a kind of Chinese medicine composition for hair growth, which can achieve the effects of promoting hair follicle growth and bacteriostasis only by adopting the rational compatibility and use of seven herbs, and can also be used for black hair, early graying, Prevention and treatment of alopecia areata, seborrheic alopecia, etc. and treatment of hair loss.
  • Another object of the present invention is to provide a preparation method of a traditional Chinese medicine composition for hair growth.
  • the combination of different extraction methods can effectively improve the conversion rate of effective substances in the formula, can effectively control the process cost, and is suitable for preparing medicines or washing. Applications in mass production of care products.
  • a hair-growing traditional Chinese medicine composition the traditional Chinese medicine composition is composed of the following parts by weight of raw materials: 10-60 parts of gallnut, 20-60 parts of Polygonum multiflorum, 20-45 parts of acacia, snakehead intestine 20 to 65 parts, 20 to 50 parts of Ligustrum lucidum, 10 to 30 parts of Chuanxiong, and 10 to 45 parts of Isatis
  • the traditional Chinese medicine composition contains the following active ingredients: tannin compounds ⁇ 85.86mg/g, anthracene Quinones ⁇ 0.29mg/g, flavonoids ⁇ 97.58mg/g, terpenoids ⁇ 1.69mg/g, tetracyclic triterpenoids ⁇ 0.95mg/g, saponins ⁇ 53.20mg/g, volatile oils ⁇ 28.36mg/g and alkaloids ⁇ 40.30mg/g.
  • the tannins are 85.86-119.27 mg/g
  • the anthraquinones are 0.29-0.85 mg/g
  • the flavonoids are 97.58-116.86 mg/g
  • the terpenoids are 1.69-5.61 mg. /g
  • tetracyclic triterpenoids are 0.95-3.06mg/g
  • saponins are 53.20-78.90mg/g
  • volatile oils are 28.36-44.21mg/g
  • alkaloids are 40.30-64.57mg/g.
  • the present invention also provides a preparation method of a hair-growing traditional Chinese medicine composition, and the following steps are carried out according to the parts by weight of the raw materials described in claim 1:
  • the traditional Chinese medicine composition contains the following active ingredients: tannins ⁇ 85.86mg/g, anthraquinones ⁇ 0.29mg/g, flavonoids ⁇ 97.58mg/g, terpenoids ⁇ 1.69mg/g, Tetracyclic triterpenoids ⁇ 0.95mg/g, saponins ⁇ 53.20mg/g, volatile oils ⁇ 28.36mg/g and alkaloids ⁇ 40.30mg/g.
  • the step of preparing the spray-dried powder of the gallnut extract comprises:
  • step (1.4a) The extract obtained in step (1.3a) is concentrated under reduced pressure at 60-70°C to obtain a clear paste with a density of 1.01-1.05. Spray drying is carried out under the condition of ⁇ 87° C. to obtain the spray-dried powder of the gallnut extract.
  • the step of preparing the spray-dried powder of the Polygonum multiflorum extract comprises:
  • step (1.4b) Concentrating the extract obtained in step (1.3b) under reduced pressure at 60-70° C. to obtain a clear paste with a density of 1.01-1.05°C. Spray drying is carried out under the condition of ⁇ 87°C to obtain the spray-dried powder of the Polygonum multiflorum extract.
  • step (1) the step of preparing the spray-dried powder of snakehead intestine extract comprises:
  • step (1.4c) The extract obtained in step (1.3c) is concentrated under reduced pressure at 60-70°C to obtain a clear paste with a density of 1.01-1.05. Spray drying is carried out under the condition of ⁇ 87° C. to obtain the spray-dried powder of snakehead intestine extract.
  • the step of preparing the spray-dried powder of the mixed extract of Ligustrum lucidum and Chuanxiong comprises:
  • step (2.2) after mixing the extract obtained in step (2.1) with water at 90°C, concentrate under reduced pressure at 55-65°C to obtain a crude extract of clear paste with a relative density of 1.01-1.05;
  • step (2.3) the crude extract of clear paste obtained in step (2.2) is spray-dried under the conditions of inlet air temperature 178 ⁇ 182 °C and outlet air temperature 83 ⁇ 87 °C, promptly obtains the mixed extract of Ligustrum lucidum and Chuanxiong of spray-dried powder.
  • the step of preparing the spray-dried powder of the mixed extract of Acacia and Isatis indica includes:
  • step (3.2) after mixing the combined filtrate in step (3.1) with water at 90°C, under the condition of 55 ⁇ 65°C, concentrated under reduced pressure to obtain a crude extract of clear paste with a relative density of 1.01 ⁇ 1.05;
  • step (3.3) the crude extract of clear paste obtained in step (3.2) is spray-dried under the conditions of inlet air temperature 178 ⁇ 182 °C and outlet air temperature 83 ⁇ 87 °C, promptly obtains the mixed extract of acacia and isatis indigotica Spray dry powder.
  • the spray-dried powder obtained in the steps (1) to (3) is mixed for ultra-fine pulverization, and then sent to the total mixer.
  • the traditional Chinese medicine composition is prepared after mixing uniformly.
  • the present invention also provides the application of the above hair growth Chinese medicine composition in the preparation of medicines or toiletries, for example, it can be used to prepare external preparations such as ointments, sprays, essences, gels, liniments, shampoos, etc.
  • the present invention has the following advantages and beneficial effects:
  • the present invention adopts Galli Fructus, Polygonum multiflorum, Acacia, Angelica sinensis, Ligustrum lucidum, Chuanxiong, Isatis chinensis, and the rational compatibility of seven herbs is used, which can nourish the Kidney and Liver, improve eyesight and black hair, detoxify and remove dampness, inhibit bacteria and reduce inflammation.
  • Comprehensive conditioning has the effect of treating hair loss and hair growth, and can be used for the prevention and treatment of premature graying of black hair, alopecia areata, and seborrheic alopecia.
  • the present invention limits the selection and content of its active ingredients in the Chinese medicine formula for the first time, and finds a Chinese medicine formula that can effectively promote the growth of hair follicles and bacteriostasis. After the combination of multiple traditional Chinese medicines, the therapeutic effect achieved by the superposition of their single medicinal effects has been verified by repeated tests. By selecting a specific traditional Chinese medicine formula, under its compatibility, its effective ingredients can be reasonably exerted, and only seven herbs can be achieved. The hair loss prevention effect of the treatment has been clinically verified and has obvious therapeutic effect.
  • the present invention is to improve the extraction efficiency of the active ingredient and achieve the purpose of treatment.
  • technological means such as continuous reflux extraction, high-speed countercurrent extraction, reflux extraction, and decoction extraction are respectively adopted, as Contributes to promoting the transformation of its active ingredients and its purpose of treating hair loss.
  • the present invention is based on the traditional Chinese medicine formula, and finds the traditional Chinese medicine formula under a specific compatibility ratio.
  • a specific extraction method a specific combination and proportion of active ingredients can be obtained, and the combination and use of the active ingredients can be obtained. It can promote the growth of hair follicles and inhibit bacteria, and then achieve the purpose of preventing hair loss.
  • the purpose of papillary cell activity is also what the present invention urgently needs to solve, namely: how to better promote the activity of hair follicle dermal papilla cells through a rationally compatible Chinese medicine formula, so as to achieve the therapeutic purpose of preventing hair loss.
  • An extract containing anthraquinone derivatives can be obtained from the root of Polygonum multiflorum, which has a nourishing and strong effect, can promote hair growth, and make white hair black.
  • Gallatia is rich in tannin (tannin) and has a significant effect on dampness and astringency.
  • the tannin in gallnut has a precipitating effect on protein, which can coagulate the protein of local tissues such as skin and mucous membranes, and play an antipruritic effect.
  • the flavonoids in the sausage have good scavenging ability to hydroxyl radicals (OH - ) and superoxide anion radicals (O 2 - ) .
  • Lecithin has a significant inhibitory effect on lipid peroxidative damage, can protect scalp cells and dermal papilla cells, and maintain a healthy scalp environment.
  • Terpenoids are high content in Ligustrum lucidum and have concentrated medicinal activity research components.
  • oleanolic acid has a significant growth-promoting effect on hair follicles. Studies have shown that oleanolic acid can delay the growth of hair follicle cells, especially dermal papilla cells. Apoptosis can delay hair loss.
  • the volatile oils in Chuanxiong mainly include ligustilide myrcene, sapinene and other alkenes and various fatty acid esters, which have the functions of improving vascular microcirculation, lowering blood pressure, and increasing cerebral blood flow.
  • the nitrogen-containing compounds in Ligusticum chuanxiong are mainly alkaloid compounds.
  • Ligustrazine has certain pharmacological effects on the blood system and cardiovascular system. It can inhibit platelet aggregation, reduce platelet surface activity, increase the surface charge of red blood cells and platelets, and improve blood flow. Sex is improved.
  • Acacia is a natural plant that is widely used in healthcare. Saponin is the main active ingredient of Acacia, has high foaming property and strong decontamination ability. It is smashed and filtered to make shampoo, which has the effect of removing fat and relieving itching.
  • Isatis is a cruciferous plant, and alkaloids are its main chemical components.
  • the water extract of Isatis has a broad-spectrum antibacterial effect. Adding it to shampoo can effectively inhibit the proliferation of bacteria on the scalp and relieve the problems of dandruff and itchy scalp.
  • the index components of anthraquinones, tannins and flavonoids in Polygonum multiflorum, Galla japonica and Liquor were enriched by high-speed countercurrent extraction method, and the pharmaceutical composition prepared after purification was significantly enhanced.
  • Ligustrum lucidum and chuanxiong, acacia and isatis are mixed and extracted respectively, which can effectively extract the active components of tetracyclic triterpenoids and volatile oils (Ligustrum lucidum and chuanxiong), as well as saponins and alkaloids (acacia and isatis), Therefore, under the reasonable combination of its effective substances, the application of the combination of traditional Chinese medicines in the field of hair loss is realized.
  • This embodiment relates to a traditional Chinese medicine composition for hair growth.
  • the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of Galla japonica, 45 parts of Polygonum multiflorum, 32 parts of Acacia, 40 parts of Anchovy intestine, 28 parts of Ligustrum lucidum, and 20 parts of Chuanxiong , 30 parts of Isatis.
  • the collected extract is concentrated under reduced pressure at 65°C to obtain a clear paste with a density of 1.03, and then the clear paste is spray-dried under the conditions of an air inlet temperature of 180°C and an air outlet temperature of 85°C to obtain Galla japonica A spray-dried powder of the extract.
  • the collected extract is concentrated under reduced pressure at 65°C to obtain a clear paste with a density of 1.05, and then the clear paste is spray-dried under the conditions of an air inlet temperature of 180°C and an air outlet temperature of 85°C to obtain Polygonum multiflorum A spray-dried powder of the extract.
  • the collected extract is concentrated under reduced pressure at 65°C to obtain a clear paste with a density of 1.02, and the clear paste is spray-dried under the conditions of an air inlet temperature of 180°C and an air outlet temperature of 85°C to obtain snakehead sausage A spray-dried powder of the extract.
  • the spray-dried powder obtained in the above process is mixed for ultrafine pulverization, and then sent to a mixer.
  • the traditional Chinese medicine composition is prepared by mixing uniformly at a feeding speed of 20 min/time, feeding 5 kg each time, and a temperature of 14 °C.
  • Example 2 On the basis of Example 1, the corresponding hair growth Chinese medicine compositions were prepared according to the following raw material ratios and process control parameters, as shown in Tables 1 to 5.
  • the effective substance detection was carried out on the hair growth traditional Chinese medicine compositions prepared in the above examples 1-8, and the main effective substances are shown in Table 6 below.
  • the present invention adopts the spray drying method to solve the problem of "thickness and blackness" of traditional Chinese medicine.
  • the color of the traditional Chinese medicine composition product prepared by the process of the invention is yellow, which greatly enhances the raw material
  • the dry powder is easy to store and transport, and the amount of addition can be precisely controlled during application (liquid preparation solvent affects application). Therefore, the present invention also has application in the preparation of medicines or toiletries, for example, it can be used to prepare external preparations such as ointments, sprays, essences, gels, liniments, shampoos, etc.
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in the preparation of ointment.
  • the ointment was prepared by using the traditional Chinese medicine composition for hair growth described in Example 1 as the active ingredient, and according to the conventional method in the art for preparing an ointment. Specifically: after dissolving the oil phase, place it in a water bath at 80°C for constant temperature insulation, and the oil phase is composed of 10.0 parts of stearic acid, 7.0 parts of ethylene glycol monostearate, 7.0 parts of white petrolatum, and 5.0 parts of liquid paraffin; The water phase heated to a slightly boiling state is added to the oil phase, and the stirring is slowly accelerated, and the temperature is kept at 80 °C and stirred for 10 minutes, wherein the water phase consists of 60.0 parts of water, 5.0 parts of sodium lauryl sulfate, and 3.0 parts of active ingredient extract. 0.2 part of ethyl hydroxybenzoate, 0.3 part of menthol, and 0.1 part of potassium sorbate; take the ointment out of the water bath and continue stirring to room temperature to obtain it
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in the preparation of sprays.
  • the spray was prepared according to the conventional method in the art for preparing the spray. Specifically: in a 40°C water bath, add 3.0 parts of the above-mentioned pharmaceutical composition to 15 parts of 80% ethanol, stir evenly, then add 0.2 part of benzoic acid, add 1 part of Tween-80, 8 parts of propylene glycol and 72.8 parts of distilled water, after stirring evenly.
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in preparing the essence.
  • the hair growth Chinese medicine composition described in Example 4 is used as the active ingredient, and the essence is prepared according to the method conventionally prepared in the art. Specifically: take 3.0 parts of the above active ingredient extract and add it to 89.3 parts of distilled water, add 0.4 part of carbomer after dissolving, add 0.2 part of benzoic acid, 2 parts of glycerol, and 5 parts of propylene glycol after homogenizing, and add three parts after stirring. 0.1 part of ethanolamine, stir evenly.
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in the preparation of gel.
  • the gel was prepared according to the conventional method in the art for preparing a gel. Specifically: take 3.0 parts of the active ingredient extract and add it to 85 parts of distilled water, add 1.5 parts of carbomer, 1.0 part of triethanolamine, 0.2 part of ethylparaben, 8.0 parts of glycerin, 0.3 part of menthol, 0.1 part of potassium sorbate, stir well.
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in the preparation of liniment.
  • the traditional Chinese medicine composition for hair growth described in Example 7 is used as the active ingredient, and the liniment is prepared according to the conventional method in the art for preparing the liniment. Specifically: take 3.0 parts of the active ingredient extract and add it to 87 parts of distilled water, add 3 parts of glycerol, 5 parts of propylene glycol, 1 part of water-soluble bisabolol, and 1 part of phenoxyethanol after dissolving, and mix well.
  • This embodiment relates to the application of the traditional Chinese medicine composition for hair growth in the preparation of shampoo.
  • the shampoo was prepared according to the conventional method of preparing shampoo in the art. Specifically: 77.8 parts of deionized water, 1.0 part of conditioner, 0.1 part of EDTA-2 sodium, 0.2 part of allantoin, 14.0 parts of fatty alcohol polyoxyethylene ether sodium sulfate, 3.0 parts of cocamidomethyl , 0.2 part of pearlescent paste, 0.6 part of silicone oil, 0.1 part of phenoxyethanol to prepare a matrix, 3.0 parts of the above-mentioned pharmaceutical composition (weight ratio) is added to the matrix, and then the auxiliary materials such as conventional amount of sodium chloride and essence are added to prepare Get shampoo.
  • the auxiliary materials such as conventional amount of sodium chloride and essence are added to prepare Get shampoo.
  • HGF hepatocyte growth factor
  • VEGF vascular endothelial growth factor
  • the C57BL/6 mice were sacrificed, the bilateral whiskers were cut off, the upper lip was sterilized with 75% ethanol, and the upper lip was washed with sterile saline. Under sterile conditions, the bilateral whisker pads were cut and placed in Hank's solution. Hair follicles, 7 to 9 hair follicles can be taken from each beard pad, and hair follicles in the complete growth phase (those with large dermal papilla, smooth and rounded, complete dermal tissue sheath and no separation from the outer root sheath) are selected for culture.
  • Blank group distilled water.
  • Test group The traditional Chinese medicine composition involved in the above Example 1 was used as the active ingredient to prepare a solution of 1200 ⁇ g/l.
  • Control group 1 spray-dried powders of anthraquinones, tannins and flavonoids obtained by continuous reflux extraction of Polygonum multiflorum, Galla japonica and Lichang and enriched by high-speed countercurrent extraction were prepared into a solution of 1200 ⁇ g/l.
  • Control group 2 The spray-dried powder was obtained by continuous reflux extraction with Ligustrum lucidum and Chuanxiong to prepare a solution of 1200 ⁇ g/l.
  • Control group 3 A solution of 1200 ⁇ g/l was prepared by spray-drying the powder with the aqueous extract of acacia and isatis.
  • the hair follicles were cultured in a 24-well plate, and William E serum-free medium was added, and incubated at 37°C under 5% CO 2 for 12 h.
  • the hair follicles with intact morphology and similar growth rate were selected.
  • Each group had 3 wells, and 20 hair follicles were added to each well. , add the liquid to be tested, and culture for 4d, 8d and 12d respectively.
  • a one-step RT-PCR amplification system kit was used.
  • a pair of primer sequences specific for mouse VEGFmRNA were 5'-CCATGAATTTGACCTCTATG-3' and 5'-AATGCTTTCTCCGCTCTGAA-3', and the amplified product was 207bp.
  • a pair of primer sequences specific for mouse HGFmRNA were 5'-CCATGAATTTGACCTCTATG-3' and 5'-ACTGAGGAATGTCACAGACT-3', and the amplified product was 278bp.
  • the products amplified by RT-PCR were electrophoresed on agarose gel with a mass fraction of 2%, stained with ethidium bromide, and the gel was density scanned under ultraviolet light to obtain the absolute density of each positive band.
  • Hair length (the distance from the base of the follicle to the top of the hair) was measured daily under an inverted microscope.
  • the products amplified by RT-PCR were electrophoresed in a 2% agarose gel, stained with ethidium bromide, and the gel was scanned for density under ultraviolet conditions.
  • the products amplified by RT-PCR were electrophoresed in a 2% agarose gel, stained with ethidium bromide, and the gel was scanned for density under ultraviolet conditions.
  • the above cell experiment results show that the combined extract has the effect of promoting the differentiation and development of hair follicle cells, increasing the volume of the hair follicle and increasing the length of the hair shaft.
  • Propionibacterium acnes, Escherichia coli, and Staphylococcus aureus were all provided by Sichuan Industrial Microorganism Collection and Management Center.
  • the bacterial solid medium consists of the following components: beef extract 4g, peptone 12g, sodium chloride 3g, agar 20g, water 1 000ml, pH 7.2-7.4.
  • Blank group distilled water.
  • Test group The traditional Chinese medicine composition involved in the above Example 1 was used as the active ingredient to prepare a solution of 0.05 mg/ml.
  • Control group 1 spray-dried powders of anthraquinones, tannins and flavonoids obtained by continuous reflux extraction of Polygonum multiflorum, gall and licorice and then enriched by high-speed countercurrent extraction were prepared into a solution of 0.05 mg/ml.
  • Control group 2 The spray-dried powder was obtained by continuous reflux extraction with Ligustrum lucidum and Chuanxiong to prepare a solution of 0.05 mg/ml.
  • Control group 3 A solution of 0.05 mg/ml was prepared by spray-drying the powder with the aqueous extract of acacia and isatis.
  • the inhibitory effect of the above experimental groups on the test bacteria was determined by the filter paper diffusion method, and 0.2 ml of each of the above four test bacteria suspensions was inoculated on the surface of the medium, and the bacteria-containing plate was made after evenly coating.
  • the filter paper was punched into a small filter paper sheet with a diameter of 10 mm with a hole punch. After sterilization, the above filter paper was soaked in the blank group, the test group, and the control group 1, 2, and 3, respectively, for 2 hours. Incubate at 30°C for 24 hours, measure the diameter of the inhibition zone by the cross method, and take the average value.
  • P. acnes was weak, mainly because the ethyl ferulate in Chuanxiong was helpful for the growth of P. acnes.
  • Blank group adopt the essence prepared except the hair growth Chinese medicine composition of this embodiment 1 as the active ingredient, and the amount of crude drug in the essence is 0g/ml. How to use: 2 to 3 times a day, the dosage is 3 to 5ml each time, how to use: Use a dropper to absorb an appropriate amount of essence into a specific discharge comb, place the comb teeth on the scalp, and press the comb with appropriate force teeth and roll smoothly until all of the single-use serum is evenly applied to the scalp.
  • Test group the essence prepared by using the hair growth Chinese medicine composition of Example 1 as the active ingredient, and the amount of crude drug in the essence is about 3 g/ml. How to use: 2 to 3 times a day, the dosage is 3 to 5ml each time, how to use: Use a dropper to absorb an appropriate amount of essence into a specific discharge comb, place the comb teeth on the scalp, and press the comb with appropriate force teeth and roll smoothly until all of the single-use serum is evenly applied to the scalp.
  • Control group 1 The essence was prepared from spray-dried powder of anthraquinones, tannins and flavonoids that were enriched by high-speed countercurrent extraction after continuous reflux extraction of Polygonum multiflorum, gall and licorice, and the amount of crude drugs in the essence was about 3g/ml . How to use: 2 to 3 times a day, the dosage is 3 to 5ml each time, how to use: Use a dropper to absorb an appropriate amount of essence into a specific discharge comb, place the comb teeth on the scalp, and press the comb with appropriate force teeth and roll smoothly until all of the single-use serum is evenly applied to the scalp.
  • Control group 2 The essence was prepared by continuous reflux extraction of Ligustrum lucidum and Chuanxiong to obtain spray-dried powder, and the crude drug amount in the essence was about 3 g/ml. How to use: 2 to 3 times a day, the dosage is 3 to 5ml each time, how to use: Use a dropper to absorb an appropriate amount of essence into a specific discharge comb, place the comb teeth on the scalp, and press the comb with appropriate force teeth and roll smoothly until all of the single-use serum is evenly applied to the scalp.
  • Control group 3 the essence was prepared by spray-drying powder of acacia and isatis water extract, and the crude drug amount in the essence was about 3g/ml. How to use: 2 to 3 times a day, the dosage is 3 to 5ml each time, how to use: Use a dropper to absorb an appropriate amount of essence into a specific discharge comb, place the comb teeth on the scalp, and press the comb with appropriate force teeth and roll smoothly until all of the single-use serum is evenly applied to the scalp.
  • Total effective rate (cure + marked effect + effective) number of cases/total number of cases ⁇ 100%.
  • Table 8 The treatment results of the essence prepared by the traditional Chinese medicine composition and the essence prepared by the control group 1-3
  • the test results show that: the 7 traditional Chinese medicines included in the prescription are Polygonum multiflorum, Galla japonica and Lichang, which are related to horn-medicine. Growth, anti-dandruff, antipruritic and root-stabilizing effects.
  • the active ingredients in Ligustrum lucidum and Chuanxiong are tetracyclic triterpenoids and volatile oils, respectively, which can promote hair follicle growth and improve scalp blood microcirculation, respectively.
  • the active ingredients of acacia and isatis are saponins and alkaloids, respectively, which can remove scalp oil and relieve dandruff and itchiness, respectively.
  • the combination of the above 7 traditional Chinese medicines can maintain a healthy scalp, promote hair follicle growth, and stabilize hair roots. It can fundamentally improve hair loss.

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Abstract

La présente invention concerne une composition de médecine chinoise traditionnelle pour la pousse des cheveux, son procédé de préparation et son utilisation associée. La composition est préparée à partir des matières premières suivantes en parties en poids : de 10 à 60 parties de Galla chinensis, de 20 à 60 parties de Polygoni multiflori radix, de 20 à 45 parties de févier épineux de Chine, de 20 à 65 parties d'Eclipta prostrata, de 20 à 50 parties de Ligustri lucidi fructus, de 10 à 30 parties de rhizome de Chuanxiong et de 10 à 45 parties d'Isatis tinctoria, et contient les ingrédients actifs suivants : des composés tanniques, des composés d'anthraquinone, des composés flavonoïdes, des composés terpénoïdes, des composés triterpénoïdes tétracycliques, des composés de saponine, des huiles volatiles et l'alcaloïde. La composition a pour effet de favoriser la croissance des follicules pileux et la bactériostase, favorise la pousse des cheveux, et peut également être utilisée pour prévenir et traiter le grisonnement prématuré des cheveux noirs, l'alopécie areata, l'alopécie séborrhéique, etc, et pour traiter l'alopécie. Pendant le processus de production, le taux de conversion de substances efficaces dans la formule peut être efficacement amélioré et le coût de production est contrôlé au moyen de l'utilisation coopérative de différents procédés d'extraction.
PCT/CN2020/117709 2020-09-03 2020-09-25 Composition de médecine chinoise traditionnelle pour la pousse des cheveux, son procédé de préparation et son utilisation WO2022047862A1 (fr)

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