WO2022019510A1 - Composition for preventing or ameliorating axillary osmidrosis, comprising corydalis remota extract - Google Patents

Abstract

Disclosed is a composition for preventing or ameliorating axillary osmidrosis, including Corydalis remota extract. The composition of the present invention can suppress the generation of bodily odor by reducing the proportion of bacteria of the genus Corynebacterium and Anaerococcus associated with axillary osmidrosis in the microbial community.

Description

Composition for preventing or improving malodor comprising Cordyceps extract

The present invention relates to a composition for preventing or improving malodor, comprising an extract of Cordyceps.

Body odor is a unique odor generated when sweat and waste products secreted from sweat glands distributed in the human body are decomposed by microorganisms, etc., and may vary according to race, gender, age, etc.

Among them, secretions from the apocrine sweat glands, which are mainly distributed in areas with a lot of body hair such as the armpits, have a high content of fat, protein, sebum, and body wastes. It may produce an unpleasant odor. Halitosis can be exacerbated by sweat and sebum secretion caused by strenuous exercise or stress, or the type of food consumed. Also, foods with strong odors, beverages containing a lot of caffeine, drinking and smoking, etc. have.

As a general method for suppressing bad odor, there are a masking method and a method using a sweat inhibitor. Among them, the masking method is a method of hiding the odor by using a different fragrance. However, if the wrong scent is used to mask the odor, it can cause a stronger odor. And although the use of a sweat inhibitor is to suppress the occurrence of bad odor by using a chemical that acts on the apocrine sweat glands, it also has a side effect of increasing sweat and bad odor in other areas where there is no problem.

Accordingly, there is a need for a natural product-based therapeutic agent that fundamentally improves bad odor by changing the microbial community that causes bad odor and has fewer side effects.

According to one embodiment, there is provided a composition for preventing or improving malodor, an external preparation for skin, and a cosmetic composition comprising a Cordyceps extract.

According to one embodiment, immersing the powdered coral color in a solvent containing alcohol to obtain an extract; And it provides a method for preparing a composition for preventing or improving malodor comprising the step of filtering the extract.

According to one embodiment, there is provided a method for preventing or treating bromhidrosis comprising administering to a subject any one of the composition, the external preparation for skin, and the composition prepared by the preparation method.

According to one embodiment, there is provided the use of any one of the composition, the external preparation for skin, and the composition prepared by the preparation method for preventing or treating bromhidrosis.

According to one embodiment, there is provided the use of any one of the composition, the external preparation for skin, and the composition prepared by the preparation method for preparing a pharmaceutical composition for preventing or treating bromhidrosis.

In one aspect of the present invention, there is provided a composition for preventing or improving malodor comprising an extract of Cordyceps.

In one embodiment of the present invention, the Cordyceps extract is to reduce the specific gravity of Corynebacterium sp. microorganisms and Anaerococcus sp. microorganisms in the skin microbial community.

In another embodiment of the present invention, the coral color extract is extracted at room temperature with a solvent containing alcohol.

In another embodiment of the present invention, the extraction is for 36 hours to 60 hours.

In another aspect of the present invention, there is provided an external preparation for skin for preventing or improving malodor comprising the composition.

Another aspect of the present invention provides a cosmetic composition comprising the composition.

In another aspect of the present invention, immersing the powdered coral color in a solvent containing alcohol to obtain an extract; And it provides a method for preparing a composition for preventing or improving malodor, comprising the step of filtering the extract.

In one embodiment of the present invention, the coral color includes flowers, leaves, seeds, and stems.

In another embodiment of the present invention, the method may further include removing the solvent after the filtering step.

In another embodiment of the present invention, the immersion may be carried out at room temperature.

In another aspect of the present invention, there is provided a method for preventing or treating bromhidrosis comprising administering to a subject any one of the composition, the external preparation for skin, and the composition prepared by the preparation method.

In another aspect of the present invention, there is provided the use of any one of the composition, the external preparation for skin, and the composition prepared by the preparation method for preventing or treating bromhidrosis.

In another aspect of the present invention, there is provided the use of any one of the composition or the composition prepared by the method for preparing a pharmaceutical composition for preventing or treating bromhidrosis.

By using the composition comprising the Cordyceps extract according to an embodiment, it is possible to effectively prevent or improve odor.

1 shows the results of analysis by GC/TOF-MS of components contained in the Cordyceps extract prepared according to one embodiment.

Figure 2 shows the results of measuring the odor intensity of the corydalis extract treated group and the non-treated group with an odor meter according to an embodiment.

Figure 3 shows the results of the sensory test before and after the test of the group without treatment with the extract of Cordyceps according to an embodiment.

Figure 4 shows the changes in the axillary microbial community of the corydalis extract treated group and the non-treated group according to an embodiment. The left ANOSIM result is R=0.584, p=0.001, and the right ANOSIM result is R=-0.077, p=0.824.

Figure 5 shows the change in the ratio of the axillary microbial community in the treatment group and the non-treated group of Corydalis extract according to an embodiment.

One aspect provides a composition for preventing or improving bronchitis, comprising the Cordyceps extract.

The scientific name of the Corydalis remota is Corydalis remota, and it is a perennial plant of the Dicotyledonous Papaveraceae Corydalis family. Cordyceps has been used as an analgesic or sedative for convulsions since ancient times, but it is not known that it has an effect on improving bromhidrosis.

The present inventors applied the Cordyceps extract to the armpits of patients with bromhidrosis, and confirmed that the Cordyceps extract has an effect of improving odor by measuring odor before and after application, a survey on whether or not odor improvement, and changes in the microbial community distribution.

The coral color may be flowers, leaves, seeds, stems, or a combination thereof, but is not limited to a specific part.

The coral color may be a living thing (fresh, not dried), dried, or powdered ones.

According to one embodiment, the Cordyceps extract can reduce the specific gravity of the microorganisms of the genus Corynebacterium sp. and Anaerococcus sp. that produce odor causing substances in the skin microbial community. . Alternatively, the Corynebacterium extract may inhibit the growth or activity of microorganisms of the genus Corynebacterium sp. and Anaerococcus sp. More specifically, it may be to lower the proportion of the genus Corynebacterium and Anaerococcus in the microbiome present in the human armpit. According to FIG. 5, when the Cordyceps extract is applied to the armpit of a patient with bronchitis, the proportion of microorganisms of the genus Corynebacterium and the microorganisms of the genus Anaerococcus in the microbial community present in the armpit is reduced. On the other hand, Propionibacterium, Chryseobacterium and Lysinibacillus microorganisms were rather increased. It is difficult to evaluate the effect of reducing the microorganisms of the genus Corynebacterium and Anaerococcus simply as a result of the direct inhibition of the Cordyceps extract against the microorganisms. This is because the change in the microbial community may be a result of increasing the growth or activity of other microorganisms, or the effect on a third microorganism that interacts with microorganisms of the genus Corynebacterium and Anaerococcus.

According to one embodiment, the coral color extract may be extracted by immersion in water, C1 to C4 lower alcohol, or a mixed solvent thereof. For example, it may be extracted with an alcohol-containing solvent, and more specifically, it may be extracted by immersion in a 70% methanol solvent.

The coral color extract may be extracted at room temperature or at a temperature, and the heating condition may be about 40 to 100°C. The room temperature may be 15 to 40 ℃, 15 to 30 ℃, 15 to 25 ℃, or 20 ℃.

The extraction may be performed by immersing the coral color in a solvent and shaking or stirring.

According to one embodiment, the extraction may be for 12 hours to 7 days, 12 hours to 3 days, 12 hours to 48 hours, or 12 hours to 24 hours.

The composition can be used by applying a predetermined amount to the underarm area and then wiping. The time left to stand after applying the composition to the armpit area is not particularly limited, and the user can appropriately adjust it in terms of ease of use and maximization of effect.

Another aspect provides a skin external preparation for preventing or improving malodor comprising a composition for preventing or improving malodor comprising the Cordyceps extract.

The external preparation for skin may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil , powder foundation, emulsion foundation, wax foundation and spray, etc., but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion (skin), a nourishing lotion (milk lotion), a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder. .

When the external preparation for skin is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/butane or a propellant such as dimethyl ether.

When the formulation is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3 -Butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation is a surfactant containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcositate, fatty acid amide ether sulfate as carrier ingredients , alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester, etc. may be used.

Another aspect provides a cosmetic composition comprising a composition for preventing or improving malodor comprising the Cordyceps extract. Details of the cosmetic composition are the same as those described above for the composition for external application for skin.

Another aspect is to obtain an extract by immersing the powdered coral color in a solvent containing alcohol; And it provides a method for preparing a composition for preventing or improving malodor comprising the step of filtering the extract.

The powdered coral color may be a powdered dried coral color.

According to one embodiment, the coral color may include flowers, leaves, seeds, stems, or a combination thereof.

The alcohol-containing solvent may include a C1 to C4 lower alcohol, and the alcohol content may be 30 to 70% (v/v).

According to one embodiment, the preparation method may be carried out further comprising centrifuging the extract to obtain a supernatant and filtering the supernatant.

According to one embodiment, the manufacturing method may further include the step of concentrating under reduced pressure after the step of filtering.

According to one embodiment, it may further include the step of removing the solvent after the filtering step. The method of removing the solvent is not particularly limited, and for example, it may be removed by a drying method such as hot air drying, cold air drying, far infrared drying, electric drying, microwave drying or freeze drying.

According to one embodiment, the immersion may be carried out at room temperature. The room temperature may be 15 to 40 ℃, 15 to 30 ℃, 15 to 25 ℃, or 20 ℃.

One aspect of the present invention provides a method for preventing or treating bromhidrosis comprising administering to a subject any one of the composition, the external preparation for skin, and the composition prepared by the preparation method.

One aspect of the present invention provides the use of any one of the composition, the external preparation for skin, and the composition prepared by the preparation method for preventing or treating bromhidrosis.

One aspect of the present invention provides the use of any one of the composition, the external preparation for skin, and the composition prepared by the preparation method for preparing a pharmaceutical composition for preventing or treating bromhidrosis.

Hereinafter, one or more specific embodiments will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of Cordyceps Extract

After powdering 100 g of a mixture of dried coral flowers, leaves, seeds, and stem bark, it was immersed in 1 L of a 70% methanol solution and extracted at room temperature for 48 hours. After the extracted solution was filtered with a filter paper, the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a coral color extract.

Example 2: Collection of skin microorganisms from a group of patients with bromhidrosis

A total of 16 subjects were recruited through the Global Medical Research Center, 8 people suffering from halitosis and 8 normal subjects not suffering from halitosis. All subjects were women in their 30s to 50s.

Eight subjects suffering from the above haemophilia applied the Cordyceps extract twice a day to the armpits (Axilla) for 1 week, and restricted the use of other cosmetics, perfumes, and antibacterial soaps during this period (hereinafter referred to as the Cordyceps extract treatment group) can). Eight normal subjects did not use Cordyceps extract and restricted the use of other cosmetics, perfumes, and antibacterial soaps for one week (hereinafter, it may be referred to as untreated group).

Skin microorganisms were collected from the armpits of the Cordyceps extract treated group before and one week after application of the Cordyceps extract. Also, in the untreated group, skin microorganisms were collected from the axilla of the untreated group before and one week after participating in the experiment. ^{Specifically, the microbes were collected by rubbing an area of about 50 cm 2} of the subject's armpit with a sterile cotton swab soaked in the preservation solution for about 3 minutes. The cotton swabs on which the skin microorganisms were collected were placed in a preservative solution containing 0.1% Tween20, 0.15 M NaCl, and 0.2% agar powder and stored in a freezer at -80°C until DNA extraction.

Example 3: Analysis of active ingredients of Cordyceps extract

In order to confirm the active ingredient of the Cordyceps extract prepared in Example 1, analysis was performed through GC/TOF-MS. The active ingredient analysis of each extract was repeated three times. And the mixture used for the analysis was also analyzed 3 times in the same way as the active ingredient analysis for quality control of the analysis.

According to FIG. 1, it was confirmed that, based on the mass, amino acids 39%, fatty acids 9%, carbohydrates (sugars) 30%, and other components (organic) 22% based on the mass of the components of the Cordyceps extract.

Example 4: Measurement of odor in the armpit

The odor intensity was measured before and after using the corydalis extract in the treated group, and before and one week after participating in the experiment in the untreated group. The odor intensity was measured at a distance of 3 cm from the subject's armpit using an odor measuring instrument OMX-ADM equipped with a gas sensor. The OMX-ADM is a device capable of measuring the strength of gases such as ethanol, acetone, hydrogen, and ammonia. The measured odor intensity is a value calculated according to the standards of the Ministry of the Environment, Japan.

Figure 2 shows the results of measuring the odor intensity before and after use of the corydalis extract treated group, and the untreated group before and one week after participation in the experiment. According to FIG. 2 , the average odor intensity of the Cordyceps extract treated group decreased from 3.4 to 3.2. On the other hand, the odor intensity of the untreated group increased from 3.1 to 3.2. Therefore, it was confirmed that the intensity of the odor can be reduced to a level similar to that of a normal person by using the Cordyceps extract of the present invention in a patient with malodorous disease.

Example 5: Evaluation of improvement in subjects

A survey was conducted on the change in odor before and after participating in the experiment to the treated group and the non-treated group of Cordyceps extract. The surveys commonly investigated personal details, compliance with application methods, and concerns about odors. And the degree of odor before and after application and whether there was improvement was investigated in the group treated with the Cordyceps extract, and the degree of odor before and after participation in the experiment was investigated in the untreated group. The degree of odor was classified into five levels: 'Very bad (5 points)', 'Bad (4 points)', 'Normal (3 points)', 'Good (2 points)', and 'Very good (1 point)'. , improvement was evaluated in two stages, positive and negative. The degree of odor and improvement was based on the individual's subjective evaluation. The survey results are shown in Tables 1 and 2 below.

Subjects treated with Cordyceps extract	Whether you are worried about odor	Degree of odor before application (points)	Degree of odor after application (points)	Whether to improve
S11	has exist	3	2	has exist
S12	has exist	4	4	has exist
S13	has exist	4	2	has exist
S14	has exist	5	One	has exist
S15	has exist	4	2	has exist
S21	has exist	3	3	none
S22	has exist	4	2	has exist
S23	has exist	4	2	has exist

untreated subjects	Whether you are worried about odor	Degree of odor before participating in the experiment	Degree of odor after a week	-
S01		none	2	3	-
S02		none	3	3	-
S03		none	3	2	-
S04	none	One	One	-
S06		none	2	2	-
S07		none	3	3	-
S09		none	2	2	-
S10		none	3	3	-

All 8 patients treated with Cordyceps extract responded that they followed the application method and had no particular side effects. According to Table 1, before the application of the Cordyceps extract, 1 subject answered 'very bad', 5 subjects answered 'bad', and 2 subjects answered 'normal' it was 7 out of 8 patients in the Cordyceps treatment group answered that the odor was improved by using the Cordyceps extract.

According to Table 2, among the 8 subjects in the untreated group, 4 subjects answered 'normal' to the level of odor before participating in the experiment, 3 subjects answered 'good', and 1 subject answered 'very good'. it was In the untreated group, there was no significant change in the response rate to the degree of odor after one week.

3 shows the results of answers to the degree of body odor felt by an individual in the survey. According to FIG. 3 , it was found that the untreated group hardly felt any change in body odor during the experiment (ns), but it was confirmed that the degree of feeling that the body odor was improved after the experiment was significantly higher in the group treated with the Cordyceps extract.

Example 6: Confirmation of changes in the microbial community of subjects with improved bromhidrosis

Changes in the community of microorganisms prepared in Example 1 were analyzed. Total DNA of microorganisms was extracted using PowerSoil DNA Pro Kit (QIAGEN, Germany), and 16S rRNA V4-V5 region (515F: 5′-GTGCCAGCMGCCGCGG-3′, 907R: 5′-CCGTCAATTCMTTTRAGTTT-3′) was subjected to PCR amplified. Amplification products were sequenced using Illumina MiSeq equipment. For sequence data, OTUs picking was performed through QIIME version 1.9.1, and bacteria were classified based on 97% or more homology with the standard nucleotide sequence registered in the Greengenes database.

Figure 4 shows the changes in the microbial community of 16 subjects participating in the experiment through the principal coordinate analysis (PCoA) method. According to FIG. 4, the difference in the microbial community before and after the use of the Cordyceps extract treated group was clear. Specifically, the position on the coordinates changed significantly before and after using the Cordyceps extract (refer to the red and blue circles in Fig. 4), and the size and position of the combined area on the coordinates also changed significantly (the red area and the position in Fig. 4). blue area). As a result of Analysis of similarities (ANOSIM), there was a statistically significant difference between the microbial community before and after the use of Cordyceps extract. On the other hand, the microbial community of 8 untreated groups did not show significant differences in location and area on the coordinates before and after participating in the experiment, and there was no statistically significant difference in the results of the similarity analysis.

Figure 5 shows the average value of the microbial community ratio of each of 8 people and 8 people untreated group of corydalis extract. According to FIG. 5, the proportion of Corynebacterium and Anaerococcus genus microorganisms that cause malodor in the microbial community by using the Cordyceps extract treated group was significantly reduced. On the other hand, the proportion of Propionibacterium, Serratia, Chryseobacterium, and Lysinibacillus strains increased. On the other hand, the ratio of microorganisms in the microbial community did not change significantly in the 8 untreated group.

However, even if two types of microorganisms of the genus Corynebacterium and the genus Anaerococcus were reduced by the use of the genus Corynebacterium, these results were obtained from the two types of microorganisms of the genus Corynebacterium and the genus Anaerococcus of the genus Corynebacterium. It cannot be concluded that it is due to a direct antibacterial or activity-inhibiting effect. Cordyceps extract may have reduced the microorganisms of the genus Corynebacterium and Anaerococcus by increasing the community of other microorganisms, or the corydalus extract may interact with microorganisms of the genus Corynebacterium and Anaerococcus. This is because, by changing the activity of a third microorganism, it may be that microorganisms of the genus Corynebacterium and Anaerococcus are indirectly reduced.

Summarizing the above experimental results, it is judged that the Cordyceps extract can reduce the proportion of bacteria in the genus Corynebacterium and Anaerococcus that produce odor substances, and thus can have an effect of improving malodor.

Claims (13)

1. A composition for preventing or improving malodor comprising an extract of Cordyceps.
2. According to claim 1,

   The coral extract is to reduce the specific gravity of Corynebacterium sp. microorganisms and Anaerococcus sp. microorganisms in the skin microbial community,

   A composition for preventing or improving bromhidrosis.
3. According to claim 1,

   The cordyceps extract is extracted at room temperature with a solvent containing alcohol,

   A composition for preventing or improving bromhidrosis.
4. 4. The method of claim 3,

   The extraction will be extracted for 36 hours to 60 hours,

   A composition for preventing or improving bromhidrosis.
5. A skin external preparation for preventing or improving malodor, comprising the composition of claim 1 .
6. A cosmetic composition comprising the composition of claim 1.
7. immersing the powdered coral color in a solvent containing alcohol to obtain an extract; and filtering the extract,

   A method for preparing a composition for preventing or improving malodor.
8. 8. The method of claim 7,

   The coral color will include flowers, leaves, seeds, and stems,

   A method for preparing a composition for preventing or improving malodor.
9. 8. The method of claim 7,

   Further comprising the step of removing the solvent after the filtering step,

   A method for preparing a composition for preventing or improving malodor.
10. 8. The method of claim 7,

    The immersion will be carried out at room temperature,

    A method for preparing a composition for preventing or improving malodor.
11. A method for preventing or treating bromhidrosis comprising administering to a subject any one of the composition of claim 1, the external preparation for skin of claim 5, and the composition prepared by the method of claim 7.
12. The use of any one of the composition of claim 1, the topical skin preparation of claim 5, and the composition prepared by the method of claim 7 for preventing or treating bromhidrosis.
13. The use of any one of the composition of claim 1 for the preparation of a pharmaceutical composition for preventing or treating bromhidrosis, or the composition prepared by the preparation method of claim 7.

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