WO2021261975A1 - Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation - Google Patents

Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation Download PDF

Info

Publication number
WO2021261975A1
WO2021261975A1 PCT/KR2021/008104 KR2021008104W WO2021261975A1 WO 2021261975 A1 WO2021261975 A1 WO 2021261975A1 KR 2021008104 W KR2021008104 W KR 2021008104W WO 2021261975 A1 WO2021261975 A1 WO 2021261975A1
Authority
WO
WIPO (PCT)
Prior art keywords
extract
mushroom
collagen
skin
plant
Prior art date
Application number
PCT/KR2021/008104
Other languages
English (en)
Korean (ko)
Inventor
조기환
Original Assignee
고려홍삼원 주식회사
조기환
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020200078772A external-priority patent/KR102535894B1/ko
Priority claimed from KR1020200182639A external-priority patent/KR102351730B1/ko
Priority claimed from KR1020210043663A external-priority patent/KR102432924B1/ko
Application filed by 고려홍삼원 주식회사, 조기환 filed Critical 고려홍삼원 주식회사
Priority to JP2022580932A priority Critical patent/JP2023532093A/ja
Publication of WO2021261975A1 publication Critical patent/WO2021261975A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a plant extract comprising plant collagen and plant mucin and a method for preparing the same.
  • Collagen is a fibrous structural protein constituting animal connective tissue, skin, bone, tendon, skin, cartilage, nails, toenails, teeth, blood vessels, and the like. Collagen accounts for about 30% of human protein, is the main component of connective tissue, and accounts for 70-90% of the dermal layer of the skin.
  • the basic structural unit of collagen is tropocollagen, which is a very large macromolecular protein of 300,000 Daltons (Da) in which a peptide having a molecular weight of 100,000 Daltons (Da) has a triple helix structure.
  • the amino acids constituting collagen are glycine, proline, hydroxyproline, and other amino acids combined with G-X-Y.
  • Collagen contains relatively low hydroxyproline in other proteins, so the hydroxyproline content is also a measure of the collagen content in the organ.
  • collagen When collagen is treated with heat or chemicals, it denatures into gelatin. It has been expected that collagen can be ingested by ingesting pork skin or chicken feet, but collagen is a high molecular weight protein of 300,000 Daltons, so the absorption rate in the body is less than 10%. have. According to the 500 Dalton Rule, in order for collagen to be absorbed into the skin, it must be less than 500 Daltons. It is known that the absorption rate of 2,000 to 6,000 daltons of pharmaceutical grade collagen is 50%, and more than 90% of collagen hydrolyzed to 500 daltons is absorbed.
  • the collagen is a fibrous protein constituting skin, cartilage, hair, nails, toenails, teeth, and blood vessels, and serves to maintain the structure of the extracellular matrix (ECM) and maintain elasticity.
  • ECM extracellular matrix
  • ROS reactive oxygen species
  • Plant collagen present in plants promotes collagen production in the body and inhibits collagen degradation by inhibiting MMP-1, a collagen-degrading enzyme.
  • vegetable collagen is rich in antioxidants that animal collagen does not have.
  • Most of the collagen products currently on the market are animal collagen extracted from fish or cattle, and there are concerns about consumers being uncomfortable to eat due to their unique smell or using mad cow disease or antibiotics.
  • Most of the vegetable collagen of the present invention has a molecular weight of 100 to 400 Daltons and is hydrolyzed to an average of 500 Daltons or less, so the absorption rate is more than 90%. can be useful for
  • the present inventors made intensive research efforts to develop a method for producing a plant extract containing a large amount of vegetable collagen that can replace the existing animal collagen.
  • viscous vegetable collagen and vegetable mucin are contained in large amounts in the extract extracted through enzyme and microbial fermentation after plant powder is immersed in a solvent.
  • the present invention was completed.
  • an object of the present invention is to provide an extract comprising vegetable collagen and vegetable mucin and a method for preparing the same.
  • Another object of the present invention is to provide a food composition or cosmetic composition for skin anti-aging, wrinkle improvement, skin barrier strengthening, or skin moisturizing comprising an extract containing vegetable collagen and vegetable mucin as an active ingredient.
  • the present invention provides a food composition for skin anti-aging, wrinkle improvement, skin barrier strengthening, or skin moisturizing comprising a plant extract as an active ingredient.
  • the plant extract includes plant collagen and plant mucin.
  • vegetable collagen refers to collagen derived from plants.
  • the extract extracts at least one herbal material selected from the group consisting of spinach, Swiss chard, carrot, tomato, astragalus, chrysanthemum, mushrooms, round hemp, lotus root, rape flower, and rapeseed it was obtained by
  • the one or more herbal ingredients may be one single herbal ingredient, or a mixture of two or more kinds of herbal ingredients.
  • the mushrooms are not strictly classified as plants, but the plant extract of the present invention is a concept including the extract of mushrooms.
  • the food composition is pomegranate, tart cherry, blueberry, garlic, mango, orange, avocado, white lily, cashew nut, hibiscus, strawberry, goldfish, kiwi, guava, pineapple, soybean,
  • An extract obtained by extracting a mixture of one or more herbal ingredients selected from the group consisting of bell pepper, raspberry, blackberry, goji berry, bokryeong, and sukjihwang is additionally included.
  • the mushrooms are white wood ear mushroom, wood ear mushroom, reishi mushroom, chima mushroom, matsutake mushroom, shiitake mushroom, truffle, situation mushroom, horseshoe mushroom, pine mushroom, chaga mushroom, neungi mushroom, roe deer At least one mushroom selected from the group consisting of oyster mushroom, oyster mushroom, and blackcurrant mushroom, but is not limited thereto.
  • the white woody mushroom belongs to the white woody mushroom and its scientific name is Tremella fuciformis. It is widely distributed in Korea, China, Japan, and tropical regions.
  • the fruiting body (mushroom) of the white-necked ear is agaric, wrinkled and split, or has the shape of an earlobe or a crest, and the size is about 10 cm. When dried, it forms a thin film, but swells when water is absorbed. It is known to have various effects such as immunity enhancement, bone health enhancement, constipation improvement, anticancer action, skin beauty, prevention of vascular disease, and prevention of anemia.
  • the present inventors have attempted to develop an extraction method according to the enzymatic treatment of the white wood ear mushroom in order to further improve the efficacy of the white wood ear mushroom and to improve the properties as a food composition.
  • the mushrooms refer to the fruiting bodies of each type of mushroom, but are not limited thereto.
  • the extract may be included in the range of 0.001 to 20% (w/w) based on the total weight of the food composition, but this is only exemplary and not limited thereto.
  • the extract contains hyaluronic acid. Accordingly, the extract of the present invention contains vegetable hyaluronic acid.
  • the plant extract which is an active ingredient of the composition, contains vegetable collagen and plant mucin, and increases the expression of collagen in the skin.
  • the plant extract of the present invention contains collagen peptides and is prepared by fermentation. Therefore, the plant extract of the present invention can be called a fermented collagen peptide composition.
  • the raw material of the plant extract of the present invention is white wood ear mushroom, it may be called a white wood ear mushroom fermented collagen peptide composition.
  • Collagen a major component of the extracellular matrix, is a protein expressed in fibroblasts of the skin and forms most of the organic materials of skin, bones, and teeth. It has various functions such as cell support (Brenneisen et al., 2002; Kim et al., 2015).
  • Collagen is known to be closely related to the formation of wrinkles in the skin, and a lack of collagen can cause wrinkles.
  • Collagen is synthesized in the form of procollagen, a precursor, and is known to be cleaved and separated from collagen molecules during collagen polymerization (Lee et al., 2015). That is, by measuring the amount of propeptide, it is possible to determine the degree of collagen biosynthesis in the cell.
  • the food composition of the present invention may include ingredients commonly added during food production, as well as the extract.
  • the additional ingredients include, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents.
  • examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.
  • the present invention provides a plant fermented food comprising the food composition.
  • the plant may be, for example, white wood ear mushroom, but is not limited thereto.
  • the present invention provides a fermented food of white wood ear mushroom.
  • the fermented white wood ear mushroom food of the present invention may include not only the fermented collagen peptide composition prepared by the above manufacturing method, but also the ingredients commonly added during the preparation of the above-described food.
  • the food composition may be prepared in the form of a beverage.
  • citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, tofu extract, jujube extract, licorice extract, etc. may be additionally included in addition to the extract of the present invention.
  • the fermented food may be a fermented white wood ear mushroom drink.
  • the food composition of the present invention includes processed forms of all natural materials such as food, functional food, nutritional supplement, health food, and food additives.
  • Food compositions of this type can be prepared in various forms according to conventional methods known in the art.
  • the extract may be prepared in the form of tea, juice, and drink to be consumed, or granulated, encapsulated and powdered.
  • beverages including alcoholic beverages
  • fruits and their processed foods eg, canned fruit, bottled, jam, marmalade, etc.
  • fish, meat and their processed foods eg, ham, sausage corn beef, etc.
  • breads and noodles eg udon noodles, soba noodles, ramen, spaghetti, macaroni, etc.
  • fruit juice various drinks, cookies, syrup, dairy products (eg yogurt, fermented milk, butter, cheese, etc.), edible vegetable oils and fats, margarine, Vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.) may be prepared by adding the extract of the present invention.
  • vegetable paste, soy sauce, sauce, etc. may be prepared by adding the extract of the present invention.
  • vegetable paste e.g., soybean paste, soy sauce, sauce, etc.
  • the present invention provides a cosmetic composition for skin anti-aging, wrinkle improvement, skin barrier strengthening or skin moisturizing comprising a plant extract as an active ingredient.
  • the extract is also characterized in that it contains vegetable collagen and vegetable mucin.
  • the cosmetic composition of the present invention contains the same extract as the above-described food composition as an active ingredient, and descriptions of common contents between the two inventions are omitted in order to avoid the complexity of the present specification.
  • the extract containing the vegetable collagen and vegetable mucin may be included in an amount of 0.001 to 20% by weight.
  • the formulation of the cosmetic is not particularly limited, but preferably skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence , nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.
  • the cosmetic of the present invention may also contain other ingredients that are formulated in conventional cosmetics.
  • Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, colorants, fragrances, A blood circulation promoter, a cooling agent, a restrictive agent, purified water, etc. are mentioned.
  • Blending components that may be added other than the above are not limited thereto, and any of the above components can be blended within a range that does not impair the object and effect of the present invention, but preferably 0.01 to 5 wt%, more preferably, based on the total weight 0.01 - 3 wt%, 0.01 - 2 wt%, 0.01 - 1 wt%, 0.1 - 3 wt%, 0.5 - 3 wt%, 1 - 3 wt%, or 2 - 3 wt%, etc. may be blended.
  • the cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.
  • the ingredients included in the cosmetic of the present invention may include ingredients commonly used in cosmetics in addition to the peptide composition as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances carrier.
  • conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances carrier.
  • the cosmetic formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc.
  • the cosmetic formulation of the present invention is a powder or a spray
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propellants such as propane/butane or dimethyl ether.
  • a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.
  • the cosmetic formulation of the present invention is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals
  • cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.
  • the cosmetic formulation of the present invention is a surfactant-containing cleansing agent
  • aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester and the like can be used.
  • alkali metal salts of fatty acids When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. are used as carrier components may be, but is not limited thereto. These may be used alone or in combination of two or more.
  • the present invention provides a method for preparing a vegetable extract comprising the steps of:
  • the plant extract according to the present invention abundantly contains vegetable collagen, plant mucin, and the like.
  • the method for preparing the extract further comprises the step of (e) obtaining a powdery extract by drying the liquid extract or the same.
  • the herbal material powder of step (a) is pulverized to a particle size in the range of 10 to 100 mesh (mesh).
  • a method of pulverizing and pulverizing food commonly used in the art may be used without limitation.
  • the herbal material is at least one selected from the group consisting of spinach, Swiss chard, carrot, tomato, astragalus, chrysanthemum, mushrooms, round hemp, lotus root, rape flower and rapeseed.
  • the herbal material is pomegranate, tart cherry, blueberry, garlic, mango, orange, avocado, white lily, cashew nut, hibiscus, strawberry, goldfish, kiwi, guava, pineapple, soybean, Bell pepper, raspberry, blackberry, goji berry, bokryeong, and additionally includes at least one selected from the group consisting of sukjihwang.
  • the mushrooms are white wood ear mushroom, wood ear mushroom, reishi mushroom, chima mushroom, matsutake mushroom, shiitake mushroom, truffle, situation mushroom, horseshoe mushroom, pine mushroom, chaga mushroom, neungi mushroom, roe deer At least one mushroom selected from the group consisting of oyster mushroom, oyster mushroom, and blackcurrant mushroom, but is not limited thereto.
  • the solvent is purified water or a C1 to C4 aqueous solution of lower alcohol, and more specifically, purified water or an aqueous ethanol solution.
  • the solvent in step (a), is added in an amount of 1 to 100 parts by weight based on 1 part by weight of the herbal herbal material powder.
  • the purified water may be added in an amount of 2 to 80 parts by weight, 2 to 50 parts by weight, 3 to 30 parts by weight, 5 to 20 parts by weight, 5 to 15 parts by weight, or 5 to 10 parts by weight, but is not limited thereto. .
  • the plant herbal material powder of step (a) is treated with an acid selected from citric acid, acetic acid, or dilute hydrochloric acid at a concentration of 1 to 80% (w/w) for 1 to 5 hours.
  • the acid is 1 to 60% (w/w), 1 to 50% (w/w), 1 to 40% (w/w), 1 to 30% (w/w), 1 to 20% (w/w) ), or 1 to 10% (w/w), but is not limited thereto.
  • the preparation method of the present invention may include neutralizing after treatment with an acid selected from citric acid, acetic acid, or dilute hydrochloric acid.
  • step (a) may include a step of adding an aqueous ethanol solution as a solvent to the plant herbal material powder.
  • the process of adding the aqueous ethanol solution as a solvent may be performed after acid treatment or acid treatment and neutralization of the above-described vegetable herbal material powder.
  • the extract of the present invention may be extracted by a known natural product extraction method.
  • extraction may be performed by cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, or heating extraction method, and specifically, extraction may be performed by hot water extraction or reflux cooling extraction method, 1 to 10 times, more specifically 2 Extraction can be repeated 7 to 7 times.
  • the extraction solvent may be water (purified water).
  • the extract may be used in the form of a crude extract extracted with a solvent, and may be purified and used with high purity.
  • the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the extract of the herbal herbal material includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, dialysis, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc., additionally carried out The fractions obtained through various purification methods are also included in the extract of the herbal herbal material of the present invention.
  • step (a) an aqueous ethanol solution is added to the vegetable herbal material powder as a solvent and extracted, followed by solid-liquid separation to obtain a liquid component, and the solid content is 60-95° C. after adding water After hot water extraction in the ethanol solution, it is mixed with the liquid component extracted with the aqueous ethanol solution.
  • step (a) a solvent is added to the herbal herbal material powder, and the pressure treatment may be performed under 0.2 to 5 atmospheres.
  • the pressurization treatment may be performed in parallel with the hot water extraction step.
  • the pressurization treatment is performed under conditions of 0.2 atm or more and less than 1 atm; pressure treatment under conditions of more than 1 atmosphere and less than 5 atmospheres; Alternatively, after being pressurized under conditions of 0.2 atm or more and less than 1 atm, it may be pressurized again under conditions of greater than 1 atm and below 5 atm.
  • the pressure treatment under the conditions of 0.2 to 5 atmospheres may be performed for 30 minutes to 2 days, and may be performed one or more times under different pressure and time conditions.
  • the pressure treatment may be, for example, pressurized under conditions of 0.2 atm or more and less than 1 atm for 30 minutes to 1 day, and then pressurized under conditions of greater than 1 atm and below 5 atm, for 30 minutes to 1 day.
  • the pressurization process under the conditions of more than 0.2 atm and 1 atm or less expands and removes (de-foaming) fine air bubbles contained in the mixture of powder and solvent so that the herbal material powder can be mixed with the solvent better, and through this Increases extraction efficiency by allowing collagen, vegetable mucin, and vegetable polysaccharide to be better extracted into the solvent.
  • the pressurization process under the conditions of more than 1 atmosphere and less than 5 atmospheres increases the extraction efficiency by increasing the solubility of vegetable collagen and vegetable mucin contained in the herbal material powder by applying high pressure to the herbal powder and the solvent.
  • the heat treatment may be performed at a temperature condition of 50-130 °C during the pressure treatment under the 0.2 to 5 atmospheres pressure condition.
  • the heat treatment process increases the solubility of useful components dissolved in the solvent, thereby increasing the extraction efficiency of vegetable collagen and vegetable mucin contained in the herbal material.
  • step (a) a solvent is added to the herbal herbal material powder, and the pressure and heat treatment are performed for 30 minutes to 2 days under conditions of 0.2 to 5 atmospheres and a temperature of 50-130 °C.
  • the present invention is not limited thereto.
  • This step is a step of adding an enzyme, lactic acid bacteria, or a combination thereof to the mixed solution prepared in step (a) and culturing.
  • the enzyme or lactic acid bacteria may be added alone and cultured, or the enzyme and the lactic acid bacteria may be added together (sequentially or simultaneously) and cultured.
  • the enzyme of step (b) is selected from the group consisting of cellulase, hemicellulase, beta-glucosidase, betaglucanase, xylanase, arabinase, protease, and amylase
  • cellulase hemicellulase
  • beta-glucosidase betaglucanase
  • xylanase arabinase
  • protease and amylase
  • the protease is at least one protease selected from the group consisting of pepsin, papain, trypsin, and elastase.
  • the enzyme may be used as a complex enzyme, such as Sumizyme, Ultimase, Viscozyme, Lyvarome A5, but is not limited thereto.
  • the enzyme may be added at a concentration of 0.01 to 3 wt%, but is not limited thereto.
  • the amount of the enzyme added is more specifically 0.1-4%, 0.1-3%, 0.1-2%, 0.1-1%, 1-4%, 1-3%, 1- 2%, 1%, 2%, or 3%, but not limited thereto. If the addition amount of the enzyme is less than 0.01%, the hydrolysis effect of the enzyme does not appear, and if it exceeds 5%, the manufacturing cost is excessive and not economical.
  • the temperature during the treatment of the enzyme is 40-70 °C, more specifically 40-65 °C, 45-65 °C, or 50-65 °C, but is not limited thereto.
  • the enzyme treatment it is preferable to perform the treatment while stirring in order to increase the contact frequency and reaction efficiency between the enzyme and the plant herbal material.
  • the stirring speed is 50-250 rpm, but is not limited thereto.
  • the polysaccharide when enzyme treatment is performed on the vegetable herbal material, the polysaccharide is reduced in molecular weight according to the hydrolysis of the polysaccharide and the taste is also improved.
  • the whole powder of vegetable herbal material is added here, the texture of the vegetable herbal material containing a large amount of vegetable collagen and the preference of the whole composition are greatly improved.
  • the extract prepared by enzymatic treatment may be called a white wood ear mushroom enzyme-treated polysaccharide extract.
  • the enzyme-treated polysaccharide extract of the white oyster mushroom can be prepared as a food composition by adding the whole powder of the white rhododendron mushroom. In this case, the texture and taste of the food composition are improved.
  • the lactic acid bacteria of step (b) is Leuconostok genus, Lactobacillus genus, Bifidobacterium genus, Enterococcus sp. strain, Lactococcus genus, or a combination thereof.
  • the Leuconostok genus bacteria is a Leukonostok mecenteroides strain
  • the Lactobacillus genus bacteria is Lactobacillus delbrooki spp. Bulgaricus, Lactobacillus helveticus, Lactobacillus permentum, Lactobacillus thermophilus, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus reuteri, or these is a combination of
  • the Lactobacillus genus fungus is Lactobacillus alementarius ( Lactobacillus alementarius ), and more specifically Lactobacillus alementarius Reuter strain ( Lactobacillus alementarius Reuter, ATCC 29643).
  • the Enterococcus sp. strain is Enterococcus faecium, Enterococcus faecalis, or a combination thereof.
  • the Lactobacillus genus bacteria are Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus reuteri, Lactobacillus permentum, Lactobacillus Helveticus, Lactobacillus brevis, Lactobacillus elementurius, Lactobacillus delbrooki bulgaricus, or a combination thereof.
  • the Bifidobacterium genus bacteria is Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium animalis lactis, or a combination thereof. to be.
  • the Lactococcus genus bacteria is Lactococcus lactis.
  • the culturing in step (b) is made for 1 to 50 days.
  • the culturing in step (b) is made for 1 day to 14 days, more specifically 1 day to 10 days, 1 day to 7 days, 1 day to 5 days, or 1 day to 3 days, but is not limited thereto.
  • the culture in step (b) is controlled within the range of pH 6 to 8, more specifically pH 6.5 to 7.5, and even more specifically 6.5 to 7, but limited thereto It can be adjusted appropriately depending on the enzyme used and the optimal culture conditions of the lactic acid bacteria.
  • the step is to remove the sludge and starch by centrifuging or filtering the culture solution of the enzyme, lactic acid bacteria, or a combination thereof prepared in step (b), and to separate the viscous supernatant, or to prepare the filtrate remaining after filtering. is a step
  • the centrifugation may be performed under suitable conditions that can be generally used for solid-liquid separation in the art, and it is sufficient as long as it can achieve the purpose of separating the supernatant from the precipitate.
  • the step is a step of inactivating and sterilizing the enzyme, lactic acid bacteria, or a combination thereof contained therein by heating the viscous supernatant or the filtrate prepared in step (c).
  • the heating in step (d) is performed at 60-150° C. for 0.5 seconds to 3 hours.
  • the heating in step (d) is performed at 61-65° C. for 30 minutes to 1 hour, or at 95-150° C. for 0.5 seconds to 30 seconds.
  • the step is a step of obtaining a liquid extract from step (d), or drying the liquid extract to obtain a powdery extract.
  • drying method natural drying, drying under reduced pressure, and drying methods of the extract that can be used in the art, such as freeze-drying, may be used without limitation, but drying under reduced pressure or freeze-drying is preferable.
  • the present invention provides a method for producing a plant composition comprising a plant extract and whole powder of a plant herbal material, comprising:
  • step (c) Mixing the above-mentioned plant extract with a powder prepared by drying and pulverizing the precipitate remaining after centrifugation or filtration in step (c), or the filtrate.
  • the extract is prepared by a manufacturing method comprising steps (a) to (d), or (a) to (e) of the above-described method for preparing the extract.
  • the plant extract may be prepared as a food composition by adding whole powder of plant raw materials. In this case, the texture and taste of the food composition are improved.
  • the present invention provides an extract comprising vegetable collagen and plant mucin, a food composition and a cosmetic composition comprising the extract, and a method for preparing the extract.
  • the extract containing vegetable collagen and vegetable mucin of the present invention contains low molecular weight collagen of 500 Da or less, and has excellent skin moisturizing effect, skin aging prevention effect, and skin wrinkle improvement effect when orally administered and applied to the skin. It can be usefully used as a raw material of functional food and cosmetics for improvement.
  • 1 is a diagram showing the results of a cytotoxicity test of the enzyme-treated extract of white wood ear mushroom of the present invention.
  • Figure 2 is a view showing the results of confirming the procollagen synthesis promoting effect of the enzyme-treated extract of white wood ear mushroom of the present invention.
  • FIG. 3 is a schematic diagram of a method for producing a fermented composition for white wood ear mushroom according to the present invention.
  • FIG. 4 is a diagram showing that the thickness of the epidermis of the UVB irradiation group (CON) was significantly increased as compared to the normal control group (NOR) as a result of H&E staining, and the epidermis thickness was decreased when the preparation of the present invention was administered to be.
  • % used to indicate the concentration of a specific substance is (weight/weight) % for solid/solid, (weight/volume) % for solid/liquid, and Liquid/liquid is (volume/volume) %.
  • Example 1-1 Preparation of Enzyme-treated Polysaccharide Extract from White Wood Ear Mushroom
  • the SUMIZYME AC (Shin Nihon Chemical, Japan) is an enzyme complex produced by Aspergillus niger (A. niger), and is a complex enzyme including cellulase, hemicellulase and beta-glucosidase.
  • the Ultimase is a complex enzyme including beta-glucanase and xylanase.
  • the Viscozyme is a complex enzyme comprising arabanase, cellulase, beta-glucanase, hemicellulase, and xylanase.
  • the Lyvarome A5 is a pectinase extracted from Aspergillus niger.
  • Example 1-2 Cytotoxicity evaluation of polysaccharide extracts treated with Enzyme-treated White wood ear mushroom
  • Each of the HDFn cells was dispensed in a 96 well plate at 5 ⁇ 10 4 cells/mL, and the concentration of each sample extract (Preparation Examples 1-1 to 1-4) prepared in Example 1-1 under Media-FBS 10% conditions. Each was treated for 72 hours. After that, after adding 1/10 times the volume of each cell culture solution, MTS lysate was added, followed by incubation at 37°C for 2 hours, and then absorbance was measured at 490 nm using an ELISA reader (Spectrostar-nano, BMG Labtech, Offenburg, Germany). did. Through this, it was tested whether each sample affects the cell viability.
  • the HDFn cells were purchased from Korea Cell Line Bank (KCLB, Seoul, Korea), and penicillin/streptomycin 100 unit/mL (10378, Invitrogen, Grand Island, NY, USA) and 10% FBS (16000, Invitrogen, Grand Island, NY) , USA) containing DMEM medium (11995, Invitrogen, Grand Island, NY, USA) at 37° C., 5% CO 2 Incubated in an incubator (MCO-17A1, Sanyo, Osaka, Japan).
  • the concentration of each polysaccharide extract treated with the enzymatic process of the white wood ear mushroom was treated in the range of 31.2 - 1,000 ⁇ g/mL.
  • all samples had an effect on cell viability from a concentration of 500 ⁇ g/mL, so the following experiments were performed at a concentration of 500 ⁇ g/mL or less showing a high viability.
  • Example 1-3 Measurement of Collagen Synthesis Promotion Effect of Enzyme-treated Polysaccharide Extract from White Wood Ear Mushroom
  • HDFn cells were cultured in a 96 well plate at 1 ⁇ 10 5 cells/well for 24 hours, and then a sample diluted stepwise with a new serum-free medium was dispensed and added again. Incubated in a CO2 incubator for hours. After obtaining the culture medium, the procedure was performed according to the manual using the procollagen type I Cpepited EIA kit (MK101, Takara Bio inc, Shiga, Japan). After mixing 20 ⁇ l of cell culture solution and 100 ⁇ L of secondary antibody in a 96 well plate with primary collagen antibody, incubate at 37° C. for 3 hours, wash 4 times with PBS, and then 450 using an ELISA reader (BMG Labtech). Absorbance was measured in nm.
  • procollagen synthesis experiment was performed using polysaccharides treated with enzyme-treated white wood ear mushroom, and in the samples treated with Viscozyme, SUMIZYME, and Ultimase, procollagen synthesis was increased from 62.5 ⁇ g/mL concentration, and Lyvarome was treated. In one extract and in the sample not treated with the enzyme, procollagen synthesis was significantly increased from the concentration of 125 ⁇ g/mL. From the above results, it can be seen that the enzyme-treated extract of white wood ear mushroom of the present invention exhibits wrinkle improvement effect by promoting procollagen synthesis to make elastic skin.
  • Example 1-4 Preparation of a food composition comprising a white wood ear mushroom enzyme-treated polysaccharide extract and white wood ear mushroom whole powder
  • Example 1-1 the Viscozyme-treated extract (Preparation Example 1-3), which had the best effect, and the white wood ear mushroom were hot water extracted and filtered, and the remaining white wood ear mushroom was dried and finely pulverized.
  • the prepared powder was mixed in a weight ratio of 1:10 to prepare a food composition (Preparation Example 1-5) containing the white wood ear mushroom extract and the white wood ear mushroom whole powder.
  • the extract that did not contain the white oyster mushroom powder was named Comparative Example 1-1 for the extract not treated with the enzyme, and Comparative Example 1-2 for the extract treated with the enzyme.
  • Example 1-5 Sensory test of a food composition comprising an enzyme-treated polysaccharide extract of a white wood ear mushroom and whole powder of a white wood ear mushroom
  • a beverage was prepared by taking a predetermined amount of each of the samples of Preparation Example 1-5, Comparative Example 1-1, and Comparative Example 1-2, and the odor, taste, and overall preference of the prepared beverage were evaluated.
  • the sensory inspector finally selected 10 people through a preliminary experiment from among those who received identification training in advance, and the sensory evaluation items were sweetness, flavor, texture and overall preference.
  • the degree of weakness was graded 1, and the degree of strength was graded 5.
  • the enzyme hydrolyzes the components such as cellulose and hemicellulose contained in the mushroom, and the effect of improving the taste and flavor can be obtained.
  • the effect of improving the overall taste can be obtained.
  • Example 2 Lactic acid bacteria and complex enzyme treatment
  • Example 2-1 Preparation of Fermented Collagen Peptide Composition for White Wood Ear Mushroom
  • the pH was adjusted so that the pH of the culture solution was 6 to 8.
  • the culture solution was filtered to remove sludge.
  • the filtered filtrate was collected and heated at 95° C. for 1 hour to sterilize the filtrate.
  • the filtrate was concentrated under reduced pressure at 50-65° C. and then freeze-dried to obtain 8 g of a powdery white wood ear mushroom fermented collagen peptide composition.
  • Example 2-2 Peptide Amino Acid Content and Component Analysis of Fermented Collagen Peptide Composition of White Wood Ear Mushroom
  • the white wood ear mushroom fermented collagen peptide composition of the present invention prepared in Example 2-1 was diluted to a concentration of 1 wt%, and the content and components of peptide amino acids constituting it were analyzed. The results are shown in Table 4 below.
  • the amino acid content of the fermented white wood ear mushroom collagen peptide composition of the present invention was 3,787.5 mg/kg based on 1wt% concentration, and it was confirmed that it contained a very high content of amino acids.
  • it contains 1372 mg/kg of Arginine, which is known to help improve blood flow by expanding blood vessels, and can be usefully used as a functional food for improving blood circulation.
  • Arginine which is known to help improve blood flow by expanding blood vessels, and can be usefully used as a functional food for improving blood circulation.
  • Example 2-4 sensory evaluation
  • the fermented white wood ear mushroom fermented beverage in which the enzyme and lactic acid strain of the present invention were cultured had the best overall preferences, such as sweetness and flavor.
  • the fermented beverage of the present invention had more sweetness and superior color and flavor compared to the comparative example in which lactic acid bacteria and enzymes were not treated, despite the same sugar content.
  • Example 2-5 Preparation of fermented collagen cosmetic composition for white oyster mushroom
  • a nutrient lotion containing 0.5% of the white wood ear mushroom fermented collagen peptide composition prepared in Example 2-1 based on the total weight was prepared at the component ratios shown in Table 6 below.
  • Example 2-6 Comparison of transdermal moisture loss, skin moisture content, skin elasticity, fine wrinkles improvement effect
  • the amount of transdermal moisture loss, the amount of skin moisture, the elasticity of the skin, and the effect of improving fine wrinkles were measured for the cosmetic composition containing the fermented collagen of the white wood ear mushroom of the present invention prepared in Examples 2-5.
  • Examples 2-5 were typically applied to the skin of the test subject's face and forearm for 4 weeks, 8 weeks, and 12 weeks respectively.
  • the applied skin condition was measured using anterior ocular imaging, Antera 3D imaging, Corneometer, Spectrophotometer CM2500d, and Skin-Visiometer. The results were averaged and shown in Table 7 below.
  • the feeling of use was evaluated by using 30 women in their 30s and 40s as a panel.
  • the cosmetic composition prepared in Example 2-5 was used as a test group, and a powder obtained by pulverizing the dried white wood ear mushroom fruiting body into 10-100 mesh particles was put in water, boiled for 4 hours, concentrated to dryness, and then powdered.
  • a cosmetic composition prepared at the same concentration as in Example 5 was used as a comparative example.
  • Each sample was applied to the face 3 times a week for 2 weeks, and the skin cosmetic effect was measured on a 5-point scale for each item according to the items shown in Table 8 below, and the results are representatively shown.
  • Example 5 comparative example overall sign 4.3 3.7 Skin feel after use 4.3 3.6 Spreadability when applied to the skin 4.2 3.6 Degree of skin irritation after use 0.5 1.1
  • Example 3-1 Preparation of extracts (Preparation Examples 3-1 to 3-16) containing vegetable collagen and vegetable mucin using plant herbal materials
  • a complex enzyme SUMIZYME, Ultimase, Viscozyme, or Lyvarome A5; denoted as S, U, V, and L, respectively
  • lactic acid bacteria Lactobacillus alimentarius Reuter ATCC 29643, 1x10 3 cfu/ml
  • the mixture was stirred once every 12 hours of incubation.
  • the pH was adjusted so that the pH of the culture solution was 6 to 8.
  • the culture solution was centrifuged to remove the precipitated sludge and starch, and the viscous supernatant was separated. The separated viscous supernatant was heated at 130° C.
  • Herbal medicine powder (g) Solvent (50 g) Enzyme (0.1 g) Lactobacillus strain (10 3 cfu/ml) 3-1 5g Purified water SUMIZYME - 3-2 5g Purified water Ultimases - 3-3 5g Purified water Viscozyme - 3-4 5g Purified water Lyvarome A5 - 3-5 5g Purified water - O 3-6 5g Purified water - O 3-7 5g Purified water - O 3-8 5g Purified water - O 3-9 5g Purified water SUMIZYME O 3-10 5g Purified water Ultimases O 3-11 5g Purified water Viscozyme O 3-12 5g Purified water Lyvarome A5 O 3-13 5g 20% ethanol aqueous solution SUMIZYME - 3-14 5g 20% ethanol aqueous solution Ultimases - 3-15 5g 20% ethanol aqueous solution Viscozyme - 3-16 5g 20% ethanol aqueous solution Lyvarome A5 -
  • Examples 3-2 to 3-5 evaluated the efficacy as a food composition
  • Experimental Examples 3-6 and 3-7 evaluated the efficacy as a cosmetic composition.
  • Example 3-2 Skin water retention, transepidermal water loss (TEWL) and erythema evaluation
  • the experimental group was a total of 6 groups (6 mice per group), a normal control group (normal, not UVB-induced), a negative control group (control, UVB-irradiated), Preparation Examples 3-1, 3-5, 3-9, and 3-13 of the treatment group.
  • SKH-1 hairless mice (SkH: HR-1) were purchased from Orient Bio and were bred.
  • the environment temperature of the animal breeding room was 22 ⁇ 2°C, the relative humidity was 50 ⁇ 10%, and the light and dark were adjusted in a 12-hour cycle. Drinking water and feed were ad libitum.
  • UVB-ultraviolet lamp T-8M; Vilber Lourmat, France
  • T-8M normal control group
  • NOR normal control group
  • UVB-ultraviolet lamp UV-ultraviolet lamp
  • the present inventors measured the intake of water and feed once a week during the period of irradiating the mouse with UV light and administering the sample in order to confirm the effect of the composition of the present invention on the intake of water and feed of the mouse. There was no significant difference in dietary intake between groups (Tables 11 and 12).
  • the present inventors used Multi Probe Adapter® MPA 6 (Courage und Khazaka, Germany) on the dorsal skin of rats irradiated with ultraviolet rays to confirm the effect of the composition of the present invention on the skin condition, the amount of moisture retention in the skin and the amount of transepidermal water loss (TEWL) and erythema were measured. Skin measurements were carried out in all groups before the start of UV irradiation, and skin measurements were performed every week from 4 weeks after UV irradiation to confirm that there was a significant difference in skin measurement results between the NOR and CON groups. The skin measurement results are shown in Tables 13 to 15, showing the results of the last measurement on the day before sacrifice.
  • Table 13 shows the amount of skin moisture retention for each experimental group of the present invention.
  • Table 14 shows the amount of transdermal water loss for each experimental group of the present invention.
  • the amount of transepidermal water loss was significantly increased by about 4.47 times in the negative control group (CON) compared to the normal control group (NOR) (p ⁇ 0.05). All sample administration groups significantly decreased transepithelial water loss compared to the negative control group (CON), and in particular, the greatest decrease in transepithelial water loss was achieved in Preparation Example 3-9 treated group, which was cultured by simultaneously treating enzymes and lactic acid bacteria. (p ⁇ 0.05).
  • the erythema level in the negative control group showed a significant increase of about 1.58 times compared to the normal control group (NOR) (p ⁇ 0.05), and the erythema level in the sample administration group was significant compared to the negative control group. decreased to
  • the extract containing vegetable collagen and vegetable mucin of the present invention exhibited positive activity in both water retention, transepidermal water loss, and erythema level. Therefore, it was found that the plant mixed extract of the present invention helps prevent skin moisture loss and damage to the skin barrier caused by UV rays, and helps the skin barrier function to function normally.
  • the present inventors photographed the dorsal skin of a rat and used a replica obtained using Visioline (VL650; CK electronic GmbH, Germany) to analyze the wrinkle pattern Thus, the area, number, length, and depth of wrinkles were analyzed. The results are shown in Table 16.
  • Example 3-4 Epidermal thickness and collagen measurement
  • the present inventors collected the dorsal skin of all mice after UV irradiation and the composition administration experiment was completed, put it in 10% formalin, and confirmed histopathological changes Hematoxylin and eosin (H&E) staining was performed, and immunohistochemistry (IHC) staining was performed to confirm collagen present in the dermis. After the stained tissue was imaged by taking a picture using an optical microscope, it was analyzed.
  • H&E Hematoxylin and eosin
  • IHC immunohistochemistry
  • the thickness of the epidermis of the UVB irradiation group was significantly increased (p ⁇ 0.05) compared to the normal control group (NOR).
  • the epidermal thickness was significantly reduced compared to the negative control group (CON), and in particular, in the group cultured by treating enzymes and lactic acid bacteria at the same time (Preparation Example 3-9), the epidermal thickness was significantly higher than that of the negative control group was confirmed to show a decreasing trend (p ⁇ 0.05).
  • Elastin a component of the skin dermis, is an elastic substance that affects the elasticity of the skin tissue.
  • the present inventors extracted about 10 mg of skin tissue from each experimental group, and then used the method provided by Fastin Elastin assay (F2000; Biocolor, UK) to elastin in the skin tissue. The amount was measured.
  • composition administration group of the present invention showed a tendency to increase the elastin content in all compared to the negative control group (CON), in particular, the group cultured by treating the enzyme and lactic acid bacteria of the present invention at the same time (Preparation Example) 3-9) showed the most significant increase compared to the negative control group (CON) (p ⁇ 0.05).
  • the extracts of Preparation Examples 3-1, 3-5, 3-9, and 3-13 of the present invention were added to the culture solution of human-derived fibroblasts to measure the total amount of collagen at the cellular level.
  • the total amount of collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme ImmunoAssay KIT).
  • human-derived fibroblasts were evaluated for cytotoxicity at concentrations of 10 ppm, 1 ppm, 0.1 ppm, 0.01 ppm, and 0.001 ppm of the test substance, and the total amount of collagen was measured by selecting a concentration without cytotoxicity.
  • the concentrations of the preparation examples of the present invention were 1 ppm and 10 ppm, respectively, and each sample was added to the culture medium of human fibroblasts and cultured for 2 days, then the culture solution was taken and the total amount of collagen at each concentration with the PICP EIA kit. was measured at 450 nm using a spectrophotometer.
  • the total amount of collagen was measured in the same manner for the fibroblast culture medium (control group) to which nothing was added and the sample to which vitamin C was added to a final concentration of 52.8 ppm.
  • the total amount of collagen was measured as UV absorbance, and the increase rate of the total amount of collagen was calculated as the ratio of the total amount of collagen relative to the control group, and the results are summarized in Table 19 below.
  • the extract of Preparation Example of the present invention generally exhibited an excellent collagen increase rate at a lower concentration than when vitamin C, which is known to have a collagen synthesis ability, was applied. Therefore, it was found that the extract of Preparation Example of the present invention can be used for skin regeneration and wrinkle improvement.
  • fibroblasts were evaluated for cytotoxicity at concentrations of 10 ppm, 1 ppm, 0.1 ppm, 0.01 ppm, and 0.001 ppm of the test substance, and a collagenase evaluation method was performed by selecting a concentration without cytotoxicity.
  • Fibroblasts which are normal human skin cells, were inoculated in a 24-well microplate so as to become 2.5x10 4 cells per well, and cultured for 24 hours in 10% serum DMEM medium and 37° C., and then 10% serum DMEM medium was removed.
  • TNF- ⁇ tumor necrosis factor- ⁇
  • MMP-1 collagen degrading enzyme
  • MMP-1 production inhibition rate (%) [1 - (Experimental group MMP-1 production amount - Negative control group MMP-1 production amount) / (Normal control group MMP-1 production amount - Negative control group MMP-1 production amount)]x100
  • a nutrient lotion (lotion) containing the extract obtained in Preparation Examples 3-1 to 3-16 was prepared according to a conventional method.
  • a softening lotion (skin) containing the extract obtained in Preparation Examples 3-1 to 3-16 was prepared according to a conventional method.
  • a nutritional cream containing the extract obtained in Preparation Examples 3-1 to 3-16 was prepared according to a conventional method.
  • hair conditioners containing the extracts obtained in Preparation Examples 3-1 to 3-16 were prepared according to a conventional method.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cosmetics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • General Preparation And Processing Of Foods (AREA)

Abstract

La présente invention concerne un extrait comprenant du collagène végétal et de la mucine végétale, une composition alimentaire et une composition cosmétique comprenant l'extrait, et un procédé de préparation de l'extrait. L'extrait comprenant du collagène végétal et de la mucine végétale de la présente invention contient du collagène de faible poids moléculaire de 500 Da ou moins, et a un excellent effet d'hydratation de la peau, un effet de prévention du vieillissement de la peau, et un effet d'amélioration des rides de la peau lorsqu'il est administré par voie orale et appliqué sur la peau, et, par conséquent, peut être utilisé en tant que matière première d'aliments fonctionnels et de produits cosmétiques pour améliorer l'état de la peau.
PCT/KR2021/008104 2020-06-26 2021-06-28 Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation WO2021261975A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022580932A JP2023532093A (ja) 2020-06-26 2021-06-28 植物性コラーゲン及び植物性ムチンを含む植物抽出物及びその製造方法

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2020-0078772 2020-06-26
KR1020200078772A KR102535894B1 (ko) 2020-06-26 2020-06-26 식물성 콜라겐을 포함하는 흰목이버섯 효소처리 저분자 다당체 추출물의 제조방법
KR1020200182639A KR102351730B1 (ko) 2020-12-23 2020-12-23 흰목이버섯 발효 콜라겐 펩타이드 조성물 및 이의 제조방법
KR10-2020-0182639 2020-12-23
KR1020210043663A KR102432924B1 (ko) 2021-04-02 2021-04-02 식물성 콜라겐 및 식물성 뮤신을 포함하는 추출물 및 조성물
KR10-2021-0043663 2021-04-02

Publications (1)

Publication Number Publication Date
WO2021261975A1 true WO2021261975A1 (fr) 2021-12-30

Family

ID=79281524

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2021/008104 WO2021261975A1 (fr) 2020-06-26 2021-06-28 Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation

Country Status (2)

Country Link
JP (1) JP2023532093A (fr)
WO (1) WO2021261975A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116920002A (zh) * 2023-05-30 2023-10-24 广州家化化学有限公司 一种具有祛红斑功效的密罗木提取物及其制备方法和应用
KR102604036B1 (ko) * 2023-09-15 2023-11-20 주식회사 토리든 식물유래 5d 복합콜라겐을 함유하는 피부 주름 개선 및 보습용 화장료 조성물
CN117694549A (zh) * 2024-02-05 2024-03-15 北京衡美金叶营养健康科技有限公司 一种保湿祛斑美白的组合物、制备方法及应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080036315A (ko) * 2006-10-23 2008-04-28 주식회사 두산 상황버섯 유산균 발효물 및 이의 제조방법
KR20080114069A (ko) * 2007-06-26 2008-12-31 전북대학교산학협력단 검정콩에서 추출한 단백질과 안토시아닌 추출액을 이용한기능성 콩소시지 및 그의 제조방법
KR20110050138A (ko) * 2009-11-06 2011-05-13 (주)청산녹수 균질화 숙성 막걸리 제조방법
KR20150024628A (ko) * 2013-08-27 2015-03-09 주식회사 더말코리아 마로부터 발효된 식물성 뮤신을 제조하는 방법 및 이를 포함하여 콜라겐 합성능, 보습, 피부 보호 및 상처 재생이 우수한 화장료 조성물
CN105524964A (zh) * 2016-02-17 2016-04-27 常州市庆发工业气体有限公司 一种银耳胶原蛋白肽的提取方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080036315A (ko) * 2006-10-23 2008-04-28 주식회사 두산 상황버섯 유산균 발효물 및 이의 제조방법
KR20080114069A (ko) * 2007-06-26 2008-12-31 전북대학교산학협력단 검정콩에서 추출한 단백질과 안토시아닌 추출액을 이용한기능성 콩소시지 및 그의 제조방법
KR20110050138A (ko) * 2009-11-06 2011-05-13 (주)청산녹수 균질화 숙성 막걸리 제조방법
KR20150024628A (ko) * 2013-08-27 2015-03-09 주식회사 더말코리아 마로부터 발효된 식물성 뮤신을 제조하는 방법 및 이를 포함하여 콜라겐 합성능, 보습, 피부 보호 및 상처 재생이 우수한 화장료 조성물
CN105524964A (zh) * 2016-02-17 2016-04-27 常州市庆发工业气体有限公司 一种银耳胶原蛋白肽的提取方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116920002A (zh) * 2023-05-30 2023-10-24 广州家化化学有限公司 一种具有祛红斑功效的密罗木提取物及其制备方法和应用
CN116920002B (zh) * 2023-05-30 2024-01-30 广州家化化学有限公司 一种具有祛红斑功效的密罗木提取物及其制备方法和应用
KR102604036B1 (ko) * 2023-09-15 2023-11-20 주식회사 토리든 식물유래 5d 복합콜라겐을 함유하는 피부 주름 개선 및 보습용 화장료 조성물
CN117694549A (zh) * 2024-02-05 2024-03-15 北京衡美金叶营养健康科技有限公司 一种保湿祛斑美白的组合物、制备方法及应用

Also Published As

Publication number Publication date
JP2023532093A (ja) 2023-07-26

Similar Documents

Publication Publication Date Title
WO2021261975A1 (fr) Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation
WO2021137677A1 (fr) Composition contenant un extrait de plante
KR102432924B1 (ko) 식물성 콜라겐 및 식물성 뮤신을 포함하는 추출물 및 조성물
WO2016013709A9 (fr) Composition pour l'amélioration de la peau, contenant du concentré de grenade en tant que principe actif
WO2017146414A1 (fr) Composition pour l'hydratation de la peau et atténuation des rides cutanées, contenant du α-terpinéol en tant que substance active
JP2008201773A (ja) 皮膚外用剤
WO2020122360A1 (fr) Procédé de préparation d'un extrait de zizania latifolia turcz. traité avec une enzyme présentant une teneur accrue en tricine, et composition de blanchissement, réduction des rides, anti-inflammatoire, antiallergique et d'hydratation, préparée avec ce dernier
WO2017222317A1 (fr) Composition ayant pour effet d'améliorer l'hydratation de la peau, d'éliminer la kératine cutanée, d'améliorer l'élasticité de la peau, d'inhiber l'érythème, d'atténuer les rides cutanées ou d'atténuer le photovieillissement cutané, contenant une ionone ou un sel de celle-ci comme ingrédient actif
KR20180100814A (ko) 흰점박이꽃무지(Protaetia brevitarsis) 유충의 단백 가수분해물, 이의 제조방법 및 이를 포함하는 조성물
WO2020235941A1 (fr) Composition de pulvérisation pour améliorer la santé du cuir chevelu contenant un composant eoseongcho
WO2018186640A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer son élasticité, l'exfolier, inhiber l'érythème ou réduire le photovieillissement cutané, contenant de l'ocimène ou un sel de celui-ci à titre de substance active
WO2018004141A1 (fr) Composition ayant pour effet d'améliorer l'hydratation de la peau, d'exfolier la peau, d'améliorer l'élasticité de la peau, d'inhiber l'érythème, d'atténuer les rides cutanées ou d'atténuer le photovieillissement cutané, contenant, en tant qu'ingrédient actif, un ou plusieurs composés choisis dans le groupe constitué du cimène, de l'acide béhénique, du 2-méthoxynaphtalène, du thymol et de leurs sels
KR101935153B1 (ko) 장수풍뎅이(Allomyrina dichotoma) 유충의 단백 가수분해물, 이의 제조방법 및 이를 포함하는 조성물
WO2018186641A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer son élasticité, l'exfolier, inhiber l'érythème ou réduire le photovieillissement de la peau, contenant du pipéronal ou l'un de ses sels à titre de principe actif
WO2013172525A1 (fr) Produits cosmétiques, produits pharmaceutiques et composition alimentaire contenant des morceaux ou un extrait d'yeux de poisson
WO2017213346A1 (fr) Composition contenant de la diosmine ou son sel comme ingrédient actif et présentant un effet d'amélioration de l'hydratation de la peau, d'exfoliation de la peau, de renforcement de l'élasticité de la peau, d'inhibition de l'érythème, de réduction des rides de la peau, ou de retardement du photovieillissement de la peau
WO2018186644A1 (fr) Composition pour réduire les rides cutanées, hydrater la peau, améliorer l'élasticité de la peau, exfolier la peau, inhiber un érythème ou réduire le photovieillissement de la peau, contenant de la jasmone ou un sel de celle-ci en tant que substance active
WO2018186643A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer l'élasticité de la peau, exfolier la peau, inhiber l'érythème ou réduire le photo-vieillissement de la peau, contenant de la cinchonine ou un sel de celle-ci en tant que principe actif
WO2017142265A1 (fr) Composition contenant de l'acide adipique utilisé comme principe actif pour l'atténuation des rides de la peau et l'amélioration de l'élasticité de la peau
WO2019059668A2 (fr) Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques
WO2018186639A1 (fr) Composition pour réduire les rides cutanées, hydrater la peau, améliorer l'élasticité de la peau, exfolier la peau, inhiber les érythèmes ou réduire le photovieillissement de la peau, contenant de l'ionol ou un sel de celui-ci en tant que principe actif
WO2014168433A2 (fr) Composition pour favoriser la croissance des cheveux, la régénération de la mélanine des cheveux gris et la régénération de la peau
WO2019045467A9 (fr) Pâte de poisson plat ayant un effet antioxydant et antihypertenseur et procédé de fabrication associé
WO2021002642A1 (fr) Composition pour prévenir ou traiter la polyarthrite rhumatoïde, comprenant du venin de serpent
WO2019017677A9 (fr) Composition comprenant du citral utilisé comme principe actif pour présenter un effet de raffermissement musculaire, de renforcement musculaire, de différenciation musculaire, de régénération musculaire ou de suppression de sarcopénie

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21828670

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2022580932

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21828670

Country of ref document: EP

Kind code of ref document: A1