WO2019059668A2 - Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques - Google Patents

Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques Download PDF

Info

Publication number
WO2019059668A2
WO2019059668A2 PCT/KR2018/011110 KR2018011110W WO2019059668A2 WO 2019059668 A2 WO2019059668 A2 WO 2019059668A2 KR 2018011110 W KR2018011110 W KR 2018011110W WO 2019059668 A2 WO2019059668 A2 WO 2019059668A2
Authority
WO
WIPO (PCT)
Prior art keywords
skin
milk
cream
group
lactobacillus
Prior art date
Application number
PCT/KR2018/011110
Other languages
English (en)
Korean (ko)
Other versions
WO2019059668A3 (fr
Inventor
강상모
김기숙
Original Assignee
건국대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020170122632A external-priority patent/KR101975671B1/ko
Priority claimed from KR1020170142730A external-priority patent/KR102015180B1/ko
Application filed by 건국대학교 산학협력단 filed Critical 건국대학교 산학협력단
Publication of WO2019059668A2 publication Critical patent/WO2019059668A2/fr
Publication of WO2019059668A3 publication Critical patent/WO2019059668A3/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a cosmetic composition
  • a cosmetic composition comprising an oil-in-milk cream fermentation broth of lactic acid bacterium as an active ingredient, specifically for prevention and improvement of skin aging; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; Skin whitening and atopic dermatitis prevention or alleviation, and the like.
  • the epidermis of the skin is a dynamic organ of the outermost body, maintains homeostasis and plays a central role in skin barrier function.
  • the skin barrier is located on the outermost layer of the skin.
  • the formation and differentiation of the epidermal cells and the decolorization process are repeated to participate in the selective permeation of the substance between the human body and the external environment.
  • These skin barriers are accelerated in damage due to rapid degradation of epidermal permeability and weakening of the natural moisturizing factor (NMF) function of the stratum corneum when physicochemical damage such as chronic ultraviolet rays and skin aging proceeds.
  • NMF natural moisturizing factor
  • atopic dermatitis a typical skin disease
  • atopic dermatitis is a chronic, recurrent inflammatory skin disease, which is caused by dry skin and pruritus, resulting in eczema and inflammatory skin lesions when it is scratched. As it progresses, Disorder or the like.
  • atopic dermatitis is a complex clinical manifestation due to genetic and environmental factors, the etiology of the disease is unclear and various treatment methods such as the use of moisturizers, antibiotics, antifungal agents, and photochemotherapy have been carried out.
  • Lactic acid bacteria have been used extensively in fermented food, pharmaceutical industry and feed industry since they have a function of suppressing harmful bacteria in the intestines, a function of form, an anticancer effect, an immunity enhancement function and a cholesterol reduction function. Especially, it has a high antimicrobial effect and antioxidant activity and is relatively stable in heat treatment, and thus plays an important role as a preservative. In addition, it has a function to protect the skin barrier by improving the lipid barrier and moisturizing effect of the skin.
  • lactic acid fermentation broth is composed of antimicrobial substances such as bacteriocin and ingredients useful for skin, and it is advantageous that the stock solution of the fermentation broth can be directly used as an external preparation for skin without being subjected to processing such as drying or crushing. It also has an excellent function as a skin application agent for restoring damaged skin barrier as well as inducing moisturization by forming an acidic skin on the skin.
  • substances contained in milk and milk cream which act as anti-inflammatory and antioxidants, and saturated fatty acids have antifungal, anti-inflammatory and antioxidative activities.
  • milk When milk is fermented with lactic acid bacteria, folic acid increases and moisture
  • substances that regulate sebum are produced, and lipid soluble vitamins that participate in protein synthesis or have antioxidative action are activated or increased.
  • the free amino acid content of the NMF is increased to achieve the proper nutritional balance of milk.
  • Bacteriocins which are known to be produced during the fermentation process, are highly heat resistant and viable, and cosmetic products made from a milk cream fermentation liquid can be antimicrobially effective and can be expected to have excellent cosmetic stability.
  • the object of the present invention is to prevent and improve skin aging; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; And at least one cosmetic composition selected from the group consisting of at least one kind selected from the group consisting of:
  • the object of the present invention is to prevent and improve skin aging; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; At least one kind of skin external agent selected from the group consisting of skin whitening and atopic dermatitis prevention or alleviation.
  • the object of the present invention is to prevent and improve skin aging; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; And at least one food composition selected from the group consisting of at least one kind selected from the group consisting of:
  • the object of the present invention is to prevent and improve skin aging; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; Skin whitening, and atopic dermatitis prevention or alleviation.
  • the present invention also provides a method for producing a fermented milk cream.
  • the present invention provides Lactococcus genus (Lactococcus spp.), genus Lactobacillus (Lactobacillus spp.) and Bifidobacterium (Bifidobacterium for the prevention and improvement of skin aging comprising milk-cream fermentation broth as an active ingredient of at least one strain selected from the group consisting of spp. For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; And at least one cosmetic composition selected from the group consisting of a skin whitening agent and an atopic dermatitis preventing or alleviating agent.
  • the present invention also relates to a method for preventing and improving skin aging comprising an oil-in-milk fermentation broth of at least one strain selected from the group consisting of Lactococcus, Lactobacillus and Bifidobacterium as an active ingredient; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; And at least one skin external agent selected from the group consisting of a skin whitening agent and an atopic dermatitis preventing or alleviating agent.
  • the present invention also relates to a method for preventing and improving skin aging comprising an oil-in-milk fermentation broth of at least one strain selected from the group consisting of Lactococcus, Lactobacillus and Bifidobacterium as an active ingredient; For preventing and improving skin damage; For preventing and improving skin elasticity and wrinkles; Skin whitening and atopic dermatitis prevention or alleviation.
  • the present invention also relates to a method for producing a cosmetic composition
  • a method for producing a cosmetic composition comprising the step of culturing a strain of at least one kind selected from the group consisting of Lactococcus genus, Lactobacillus genus and Bifidobacterium genus in an oil cream medium to obtain a fermentation broth, For prevention and improvement of aging; for prevention and improvement of skin damage; For preventing and improving skin elasticity and wrinkles; Skin whitening and atopic dermatitis prevention or alleviation.
  • the present invention also provides a method for producing at least one milk cream fermentation broth.
  • Lactobacillus of the present invention in Lactococcus (Lactococcus spp.), genus Lactobacillus (Lactobacillus spp.) or Bifidobacterium (Bifidobacterium
  • the cream fermented milk of the strain of the present invention has the effect of preventing the skin aging by improving the skin roughness, strengthening the skin flexibility and improving the skin elasticity. It also prevents and improves skin damage by increasing the moisture content of the face and neck skin surface, increasing the oil content, decreasing the transepidermal water evaporation (TEWL), and decreasing the skin roughness. It also promotes skin elasticity, reduces wrinkle depth, and prevents skin wrinkles and wrinkles.
  • TEWL transepidermal water evaporation
  • the composition can be usefully used in the cosmetics, external preparation for skin or foods.
  • Figs. 1 to 4 are diagrams showing examples of the prevention and improvement of skin aging of various milk cream fermentation broths of the present invention, prevention and improvement of skin damage, reduction of skin elasticity and prevention and improvement of wrinkles, skin whitening enhancement effect and alleviation of lesions of atopic dermatitis It is also confirmed.
  • Lactococcus Lactococcus spp.
  • genus Lactobacillus Lactobacillus spp.
  • Bifidobacterium for the prevention and improvement of skin aging comprising milk-cream fermentation broth as an active ingredient of at least one strain selected from the group consisting of spp.
  • at least one cosmetic composition selected from the group consisting of a skin whitening agent and an atopic dermatitis preventing or alleviating agent.
  • the milk cream fermentation broth of the present invention is a fermentation broth obtained by culturing Lactococcus spp., Lactobacillus spp. And Bifidobacterium spp.
  • a concentrated fermentation broth Means a dried product, a cultured filtrate, a concentrated culture filtrate, or a dried filtrate, and may be a cultured filtrate containing the strain or the strain after the strain has been removed.
  • the fermentation broth is not limited in its formulation, and may be, for example, a liquid, an emulsion, or a solid.
  • the fermentation broth is prepared by mixing milk-cream, milk, skim milk, Lactobacilli MRS medium, LB (lysogeny broth) medium, R2A medium (MB-R2230) and casein hydrolyzate Containing medium, preferably a milk cream, milk or Lactobacilli MRS medium, or a culture medium for culturing the strain of each strain, but the present invention is not limited thereto.
  • the milk cream is at least one kind selected from the group consisting of milk fat separated from raw milk and milk, concentrate of fat-holding lotion, processed milk cream and powdered milk cream, and generally has a single cream having a milk fat content of 30 to 40% , Light cream with 10 ⁇ 20% milk fat, table cream with 20 ⁇ 30% milk fat, milk fat with 48 ⁇ 60% milk fat Table cream, sour cream, whipping cream (whipped cream) with more than 18% of milk fat, which is a processed cream with food or food additives added to the above milk-cream. ), Or powdered milk cream in which a food or a food additive is added to the milk cream and powdered and made into a powder of milk fat of 50% or more can be used instead of fresh cream.
  • the milk is at least one selected from the group consisting of crude oil, non-fat milk, low-fat and skim milk added with a milk fat content of 1 part by weight or less, and the milk is cow, sheep, goat, But is not limited to, mammalian milk origin, such as horse, donkey, water buffalo, or camel.
  • the milk cream fermentation broth may be a fermented milk cream fermented with various lactic acid bacteria or a mixed fermentation broth of a crude fermented broth of a lactic acid bacterium or a lactic acid bacterium fermented with various lactic acid bacteria or a mixture of fermented milk cream fermented with various lactic acid bacteria Milk cream fermentation broth, or various milk cream broth fermentation broths.
  • the milk cream fermentation broth may be subjected to intermittent sterilization treatment, and may be applied to a variety of milk cream fermentation broths, but is not limited thereto.
  • the intermittently sterilized milk cream fermentation liquid may be intermittently sterilized by heating the milk cream fermented with the various lactic acid bacteria at a temperature of 80 ° C or more for 15 minutes or more, but is not limited thereto.
  • Lactobacillus The genus Lactobacillus (Lactobacillus spp.), Bifidobacterium (Bifidobacterium spp.), in Lactococcus (Lactococcus milk, cream, skim milk, Lactobacilli MRS medium, LB (lysogeny broth) medium, R2A medium (MB-R2230) and casein male
  • a culture medium containing at least one of casamino acid and casamino acid may be subjected to the concentration under reduced pressure, and one or more kinds of the lactic acid bacteria fermentation broth may be added to a variety of milk cream fermentation broth.
  • the present invention is not limited thereto.
  • the concentration of the various lactic acid bacteria fermentation broth can be reduced by subjecting the fermented lactic acid fermentation broth fermented with the above various lactic acid bacteria to intermittent sterilization and then heating at 50-70 ° C or more for 50 minutes or more to concentrate the fermentation broth twice or more under reduced pressure.
  • the diverse creamy medium fermentation broth comprises at least one member selected from the group consisting of yeast extract, MRS broth, LB medium, R2A medium, casein hydrolyzate-containing medium and commercial lactobacillus fermentation broth in an amount of at least 0.1 wt%
  • the resulting milk cream can be fermented into a single or at least one strain selected from the various lactic acid bacteria, but is not limited thereto.
  • the Lactobacillus spp. May be selected from the group consisting of Lactobacillus rhamnosus , Lactobacillus plantarum , Lactobacillus paracasei , Lactobacillus gasseri , Lactobacillus sp. del the group consisting of Brewer station (Lactobacillus delbrueckii subsp.
  • Lactobacillus subtilis strain for the prevention and alleviation of skin aging, skin damage or skin elasticity and prevention and improvement of wrinkles, skin whitening enhancement, prevention, alleviation and improvement of atopic dermatitis.
  • Lactococcus genus (Lactococcus Lactococcus lactis is a strain of Lactococcus lactis which is used for the prevention and alleviation of skin aging, skin damage or skin elasticity and prevention of wrinkles, improvement of skin whitening and prevention, alleviation and improvement of atopic dermatitis, But is not limited thereto.
  • the skin aging can be prevented or prevented by at least one effect selected from the group consisting of an increase in the moisture content of the skin surface, an increase in the amount of oil retained, a decrease in transepidermal water evaporation, a reduction in skin roughness, a skin elasticity enhancement, And this effect can also affect prevention and improvement of skin damage or skin whitening enhancement.
  • the skin damage is caused by the effect of one or more kinds selected from the group consisting of increase in moisture content of the skin surface, increase in oil content, decrease in transepidermal water evaporation amount, decrease in skin roughness, It is possible to lower the sensitivity and prevent or ameliorate the damage of the skin barrier, and the above effect may also affect improvement of skin aging, reduction of skin elasticity and prevention and improvement of wrinkles, or improvement of skin whitening.
  • the skin whitening can be improved by at least one effect selected from the group consisting of improvement of stain, dullness and senile black spots, reduction of melanin index of skin surface and improvement of skin tone.
  • the effect can be prevented by skin aging, And improvement of the skin elasticity and prevention and improvement of wrinkles.
  • the inventors of the present invention have found that, by using a fermentation broth in which a fermented liquid milk cream fermented with various lactic acid bacteria and a fermented milk cream fermentation broth fermented with each strain are mixed at various ratios, As a result, it was found that the milk cream fermentation liquid using the lactic acid bacterium of the present invention increased the moisture retention and oil retention on the surface of the face and neck skin, The skin melanin index is decreased to improve the skin tone and exhibits a whitening effect on stains, dullness and senile black spots. In addition to reducing the transdermal moisture evaporation amount and skin roughness, it also increases skin elasticity and reduces the depth of wrinkles, , Prevention and improvement of skin damage, or reduction of skin elasticity and prevention and improvement of wrinkles Respectively.
  • the inventive Lactobacillus lambosus was orally administered, and after the improvement of the skin damage or skin aging, the change rate was slightly lower than that of the Lactobacillus laminosis oil cream fermentation broth (B2), but the skin damage or the skin aging
  • B2 Lactobacillus laminosis oil cream fermentation broth
  • the composition of the present invention is useful as a measure for evaluating skin barrier damage or aging of skin, which is an increase in moisture retention and oil retention on facial skin and neck skin surface, decrease in transdermal moisture evaporation and skin roughness, It is possible to prevent skin irritation or aging of the skin as well as to reduce the melanin index of the skin surface and improve the skin tone while at the same time improving the whitening effect such as stain, And thus can be effectively used as a cosmetic composition and an external preparation for skin.
  • the inventors of the present invention respectively confirmed the effect of atopic treatment on the fermented milk cream fermented with various lactic acid bacteria.
  • the milk cream fermented milk containing the lactic acid bacteria increased the skin moisture content, And decreased the amount of transdermal water evaporation, thereby inhibiting the lesion development of atopic dermatitis and contributing to the suppression of lesion and the inhibition of recurrence. That is, the milk cream fermentation liquid of the present invention is effective in improving the skin moisture content, decreasing the pH value of the skin, stabilizing the skin or reducing the amount of percutaneous water evaporation leading to enhancement of the skin flexibility. As a result, Or prevention.
  • the composition of the present invention is effective in suppressing the relief and recurrence of lesions such as dry skin, pruritus, eczema, inflammatory skin lesions and subacute lesions such as exudates
  • the present invention provides a cosmetic composition for preventing, alleviating and improving atopic dermatitis, an external preparation for skin, or an oral preparation for preventing or treating atopic dermatitis, For example.
  • the cosmetic composition can be used for oral or skin application, for example.
  • the mixture ratio (w / g) of the live cells or dead cells is used for oral administration, and the ratio of the live cells or dead cells is used for the prevention of skin aging, skin damage or skin elasticity reduction and prevention of wrinkles and skin whitening, To achieve the purpose of prevention, improvement or symptom relief.
  • the cosmetic composition may be prepared in any form conventionally produced in the art and may be in the form of, for example, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- , A powder form foundation, an emulsion foundation, a wax foundation, a spray, and a mixture thereof, or a cosmetic form such as a soap, a foam cleanser, a body cleanser, a shampoo, etc. , And the like, but the present invention is not limited thereto.
  • composition of the present invention when used as a cosmetic composition, it can be used as a cosmetic composition such as a softening agent, a convergent lotion, a nutritional lotion, an emulsion, a lotion, a cream, an essence, a gel, a pack, a massage cream, a body lotion and cream, Basic cosmetics selected from water and foam cleansers, body cleansers and the like; Make-up cosmetics selected from sunscreen creams, sun oils, sunblocks, makeup bases, foundation, powders, lipsticks, eye creams, eyeliners, mascara, eyebrow pencils; Hair cosmetics selected from shampoo, rinse, color rinse, styling agent, hair treatment agent, hair coloring agent, hair pack, hair mousse, hair liquid, pomade and hair tonic, scalp treatment and the like; Cosmetics for facial cosmetics selected from the group consisting of hair lotion, hair lotion, aftershave lotion, shaving cream, and the like, and the formulations and forms of the cosmetic composition of the present invention are not limited by the formulations.
  • the cosmetic composition may contain all kinds of ingredients, such as a carrier and an additive, conventionally known to be suitable for each formulation of the cosmetic composition, . More specifically, it may be purified water, distilled water, flower, fermented filtrate, fermentation broth, ethanol or the like which is mixed or added to cosmetics.
  • a carrier and an additive conventionally known to be suitable for each formulation of the cosmetic composition, . More specifically, it may be purified water, distilled water, flower, fermented filtrate, fermentation broth, ethanol or the like which is mixed or added to cosmetics.
  • the present invention in Lactococcus (Lactococcus spp . ), Lactobacillus genus (Lactobacillus spp.) And Bifidobacterium (Bifidobacterium spp . )
  • Lactococcus Lactococcus spp .
  • Lactobacillus genus Lactobacillus spp.
  • Bifidobacterium Bactasspp .
  • For preventing or ameliorating skin aging comprising milk-cream fermentation broth as an active ingredient;
  • at least one skin external preparation selected from the group consisting of at least one kind selected from the group consisting of prevention and improvement of skin elasticity reduction and wrinkle, skin whitening and atopic dermatitis prevention, alleviation and improvement.
  • the external preparation for skin refers generally to the entirety of a composition used for external use.
  • the external preparation for skin refers to various cosmetics such as cosmetic compositions including various cosmetics such as basic cosmetics, makeup cosmetics, hair cosmetics, And the like, and it is preferably a cosmetic composition, more preferably a functional cosmetic composition or a functional cosmetic composition.
  • the external preparation for skin may contain a component that is usually formulated in an external preparation according to the intended use and the properties of the composition for external use, specifically, a moisturizer, an ultraviolet absorber, a vitamin, an animal extract, a digestive agent, a whitening agent, a vasodilator, A hormone agent and the like may be further added.
  • the formulations of the external preparation for skin may take any appropriate form depending on the intended use and the nature of the composition for external application. Specific examples thereof include aqueous solutions, solubilizers, emulsions, emulsions, gels, pastes, ointments, A two-layer system, a water-oil-powder three-layer system, and the formulations and the form of the external preparation of the present invention are not limited by the formulations.
  • the external preparation for skin may further include a mechanism necessary for infiltrating or transferring the active ingredient into the skin tissue.
  • the present invention relates to a method for preventing and improving skin aging comprising an oil-in-milk fermentation broth of at least one strain selected from the group consisting of Lactococcus, Lactobacillus and Bifidobacterium as an active ingredient; For preventing and improving skin damage; And at least one food composition selected from the group consisting of at least one kind selected from the group consisting of at least one kind selected from the group consisting of:
  • the active ingredient may be added to a health supplement such as food, beverage or the like.
  • Examples of foods to which the above substances can be added include drinks, various beverages, alcoholic beverages and vitamin complexes, dairy products including ice cream, dairy products and dairy products, and all of the health functional foods in the conventional sense.
  • the milk cream fermentation broth of the present invention can be added as it is to the food or can be used together with other food or food ingredients, and can be suitably used according to conventional methods.
  • the amount of the active ingredient to be mixed can be suitably determined according to the intended use (for prevention or improvement). Generally, the amount of the compound in the health food may be 0.1 to 90 parts by weight of the total food. However, when it is intended for health and hygiene purposes or for the purpose of health control, the active ingredient may be used in an amount below or above the above range.
  • the health functional beverage composition of the present invention has no particular limitation on other ingredients other than the above-mentioned compounds as essential ingredients, and may contain various flavoring agents or natural carbohydrates as additional ingredients such as ordinary beverages. Natural flavors and synthetic flavors can be advantageously used as flavors other than those described above.
  • the milk cream fermentation broth of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and heavy stabilizers (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.
  • the fermentation broth of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used alone or in combination of at least one selected.
  • the present invention provides a method for producing a fermented milk-derived fermented milk, comprising: culturing at least one strain selected from the group consisting of Lactococcus genus, Lactobacillus genus and Bifidobacterium genus in an oil cream medium to obtain a milk cream fermentation liquid using various lactic acid bacteria; Subjecting the above-mentioned milk-containing fermentation broth to an intermittent sterilization (Tyndallization) treatment alone or by treating at least one selected product with a variety of milk-derived fermentation broth to obtain a variety of milk-derived fermented broth; Lactobacillus sp., Lactobacillus sp., Bifidobacterium sp.) Are mixed with milk-cream, milk, skim-milk, Lactobacilli MRS medium, LB l of lactic acid fermentation broth obtained by culturing at least one selected from the group consisting of lysogeny broth, R2A medium (MB-R2230), casein hydrolyzate-
  • the present invention provides a method of producing at least one oil-in-milk fermentation broth selected from the group consisting of the step of obtaining a medium fermentation broth, and specifically a method of preventing and improving skin aging, preventing and improving skin damage, In order to prevent skin whitening and prevent, alleviate and improve atopic dermatitis, the production method is not limited, and the milk cream fermentation liquid prepared by the above method is also not limited.
  • composition of the present invention can be used as an anti-aging agent that has been known up to now, specifically, a skin anti-aging agent, to prevent and improve skin aging, to prevent and improve skin damage, to reduce skin elasticity and to prevent and improve wrinkles, It can be used in combination with an agent for preventing and improving aging, preventing and improving skin damage, reducing skin elasticity, preventing and improving wrinkles, and improving skin whitening. Or for the purpose of preventing, ameliorating or alleviating symptoms of atopic dermatitis, it may be used in combination with atopic dermatitis prevention and amelioration or symptom relief.
  • Salicinius KCTC 3600 Lactobacillus fermentum KCTC 3112, and Lactobacillus acidophilus KCTC 3140 at a concentration of 1 ⁇ 10 6 cfu / ml And then cultured in a 37 ° C. incubator at a constant temperature for 72 hours to prepare a milk cream fermentation broth.
  • Lactobacillus rhamnosus KCTC 5033 milk cream fermentation broth was used as a control for the milk cream fermentation solution of the various lactic acid bacteria strains.
  • Lactobacillus lambosus was inoculated with 1 x 10 6 cfu / ml of Lactobacillus lambosus strain pre-cultured to 1 L in milk cream containing 35% or more milk fat, then cultured in a 37 ° C incubator for 72 hours, A fermentation broth was obtained.
  • the fermented broth of Lactobacillus laminosus milk obtained in the same manner as in ⁇ Example 1> was heated at a constant atmospheric pressure of 80 ° C or more for 15 minutes or more, and then an intermittently sterilized milk cream fermentation broth was obtained.
  • Lactobacilli MRS or Lactobacillus lambsosus
  • Lactococcus lactis and Bifidobacterium bifidum pre-cultured in 1 l skim milk were inoculated at a concentration of 1 ⁇ 10 6 cfu / ml or more, cultured in a 37 ° C. incubator for more than 24 hours
  • Lactobacillus laminocus fermentation broth Lactococcus lactis and Bifidobacterium bifidum fermentation broth, respectively.
  • the fermentation broth of the lactic acid bacteria was intermittently sterilized by warming the fermentation broth at a temperature of 80 DEG C or higher for 15 minutes or more, and then heating the fermentation broth at 60 DEG C or higher for 60 minutes or longer.
  • a milk cream yeast medium containing 99% by weight of milk cream containing 35% or more milk fat and 1% by weight of a yeast extract (Yeast extract, Becton, Dickinson & Co, Calif., USA), 1 L of lactobacillus lanxos 6 cfu / ml or more, and cultured in a 37 ° C. incubator at a constant temperature for 24 hours or more to obtain a milk cream yeast fermentation broth.
  • yeast extract Yeast extract, Becton, Dickinson & Co, Calif., USA
  • Lactobacilli MRS broth (Becton, Dickinson & Co, Calif., USA) in 95% by weight of milk cream containing 35% or more milk fat, Lactobacillus lambosus 1 ⁇ 10 6 cfu / ml, and cultured in a 37 ° C. incubator at a constant temperature for 24 hours or more to obtain a milk cream MRS fermentation broth.
  • Lactococcus lactis was inoculated into milk cream yeast medium and milk cream MRS medium, respectively, in the same manner as above to obtain Lactococcus lactis milk yeast fermentation broth and Lactococcus lactis milk cream MRS fermentation broth, respectively.
  • Lactobacillus plantarum which had the lowest variation among the fermented milk-fermented broth fermented with various lactic acid bacteria, was inoculated into milk cream yeast medium and milk cream MRS medium, respectively, to obtain Lactobacillus plantarum milk yeast fermentation broth, Lactobacillus plantarum milk cream MRS fermentation broth respectively.
  • Emulsions (N1 to N8) and lotions (K1 to K10) containing the fermented milk cream fermented with various lactic acid bacterial strains of Examples 1 to 4 and intermittent sterilization (N10 to N13) and lotions (K12 to K16) containing various lactic acid fermentation broths of Example 3 and the lotion (K12 to K16) and the lotion (K11) containing the fermented broth of Lactobacillus laminosus milk cream (P1 and P2) and lotions (M1 to M6) of the creamy medium fermentation broth fermentation broth of Example 4 and the emulsion and the lotion (B2) containing the fermentation broth of Lactobacillus lansosus milk cream as a control were prepared as follows [ The emulsion of [Table 1] was used for the face and neck skin tests of aging skin, and the lotion of [Table 2] was used for the test of atopic dermatitis lesions, respectively Respectively.
  • Emulsion Ingredient Unit (g) A Olive liquid One Napre One W Fermented sterilized milk cream (B2, N1 ⁇ N13, P1, P2) 80 Sterile water 18 Total weight 100 Abbreviation: A, additives (olive liquid, olive oil peg-7 esters 99.9%, clear & yellowish liquid type, Italy; Napre, Zanthoxylum piperitum fruit extract & pulsatilla koreana extract & usnea barbata extract, water soluble liquid type, Koera); W, water base (B2, fermented milk cream with KCTC 5033; N1, fermented milk cream with KCTC 3115; N2, fermented milk-cream with ATCC 8014; N3, fermented milk cream with KCTC 3510; N10, fermented milk cream with KCTC 3635; N10, fermented milk cream with KCTC 3594; N7, fermented milk cream with KCTC 3112; N9 and K11, tyndalized fermented milk-cream broth with
  • Fermented sterilized milk cream (B2, K1 to K16, M1 to M6) 75 O Grape seed oil 19 Olive derived wax 5 A Napre One Total weight 100 Fermented milk cream with KCTC 5033; fermented milk cream with KCTC 3115; K2, fermented milk-cream with ATCC 8014; K3, fermented milk cream with KCTC 3510; Fermented milk-cream with KCTC 3160; K6, fermented milk-cream with KCTC 3594; K7, fermented milk-cream with KCTC 3594; K7, fermented milk-cream with KCTC 3600; : K10, fermented milk-cream with KCTC 3202); K11, tyndalized fermented milk-cream broth with KCTC 5033; K12, mixture, added 10% concentrated fermented broth with KCTC 5033 to fermented milk-cream with KCTC 5033; K13, mixture, added 20% 10-fold concentrated fermented broth with KCTC 5033 to fermented milk
  • Lactobacilli MRS or Lactobacillus lambatus (KCTC 5033), which had been preincubated with 1 l skim milk, was inoculated at a concentration of 1 ⁇ 10 6 cfu / ml or more in the same manner as in Example 4, And cultured to obtain a lactobacillus lanxosus fermentation broth. Then, the Lactobacillus laminocus fermentation broth was heated at 60 DEG C or lower for 1 hour or more, concentrated under reduced pressure, and then lyophilized and powdery, was used as the lactobacillus Lamb- nosus viable bacteria.
  • the oral Lactobacillus rhamnosus live cells were packed in capsules containing 100% by weight of Lactobacillus laminocyte cells, the dose was 500 mg / capsule, the viable cell count per capsule was 10.0 ⁇ 10 10 CFU or more, Lactobacillus Lactobacillus Lamb- nosus was prepared by filling a capsule with 100% by weight of Lactobacillus rhamnosus cells.
  • the dose was 500 mg / capsule, and the number of cells per capsule was 10.0 ⁇ 10 10 CFU or more.
  • oral mixed Lactobacillus lambatus mixed bacteria were prepared by mixing 50% by weight of the living cells and 50% by weight of the lactobacillus rhamnosus cells, and then filling the capsules with a dose of 500 mg / capsule.
  • the number of live cells per capsule was 5.0 ⁇ 10 10 CFU or more, and the number of microorganisms was 5.0 ⁇ 10 10 CFU or more, and the total weight was 10.0 ⁇ 10 10 CFU or more, respectively.
  • test period was a total of 7 weeks (49 days).
  • the skin surface changes of the clinical investigator were evaluated twice before and after the clinical trial using a digital camera (SAMSUNG, VLUU PL150, Korea) two times before and after the clinical trial.
  • images were taken at the same location designated by the same photographer at the designated photographing site.
  • the device evaluation using the skin measuring device was performed twice before and after the clinical test using the Cutometer Dual MPA 580 (Multi Probe Adapter system, C + K Electronic GmbH, Cologne, Germany) The same person was measured for objective results, and the same person was recorded before and after the clinical trial.
  • the skin measurement room set at 20 ⁇ 25 °C room temperature and relative humidity of 40 ⁇ 60%, which is suggested as optimal temperature and humidity condition for measuring the accurate state of skin, .
  • Forehead, right cheek, and right eye were used to observe the change of the facial skin, and the center of the neck was divided into two parts in order to observe the change of the neck skin.
  • Skin side moisture content, skin surface sebum content, transepidermal water evaporation (TEWL), melanin index and skin surface (TEWL) were determined as the side of the neck. roughness and skin elasticity were measured five times at each site and the mean value was taken and the average of each item of facial skin and neck skin was taken as a final measurement value to evaluate skin surface change.
  • the area of severity of the lesion of the test subject was determined as the measurement site, and the skin moisture content, skin acidity (pH), and transdermal moisture evaporation were measured and displayed individually. Objective evaluation.
  • Lactobacillus laminosus B2 as a control group as well as Lactobacillus lactis (N1), Lactobacillus plantarum (N2), Lactobacillus paracasei (N3)
  • the milk cream fermentation fermented with Gasseri N4), Lactobacillus delbrueckii (N5), Lactobacillus lutea (N6), Lactobacillus perfumeum (N7) and Bifidobacterium bifidum (N8)
  • Example 2 and Example 3 were variously contained After the emulsion prepared by the method shown in [Table 1] of Example 5 was applied, changes in moisture content of the skin surface before and after the test were measured. As a result, as shown in Tables 15 and 16 below, it was confirmed that the oil-cake fermentation broth and the oil-cream fermentation mixture, which were intermittently sterilized or concentrated under reduced pressure, increased the moisture content of the skin surface.
  • Lactobacillus lambosus (B2) used as a control group but also intermittently sterilized milk cream fermentation broth (N9), 90% by weight of lactobacillus lambosus milk cream fermentation broth, and lactobacillus lambosus fermentation concentrate 10 (N11) mixed with 90% by weight of a mixed milk cream fermentation broth (N10), 90% by weight of a mixed milk cream fermentation broth (N10), 90% by weight of a lactobacillus laminosse oil cream fermentation broth and 10% by weight of a lactococcus lactis fermentation concentrate, (N12) mixed with 10% by weight of a Bifidobacterium bifidum fermented concentrate were used to increase the moisture content and oil content of the facial skin and neck skin surface, reduce the melanin index of the skin surface, The skin tone is improved, whitening effect in which the spots, dullness, senile black spots, etc. are lightened or improved, the reduction of TEWL and skin roughness, The skin elasticity and skin elasticity, the prevention and improvement
  • lactobacillus laminosus milk cream fermentation broth was mixed with 25% by weight of fermentation broth of lactococcus lactis milk cream, 25% by weight of lactobacillus gasseri oil cream fermentation broth, and 25% by weight of fermentation broth of lactobacillus delbrueckii cream
  • the moisture content and oil content of the facial skin and neck skin surface are increased, the melanin index of the skin surface is reduced, skin tone is improved, whitening, It has been found that it contributes to the reduction of the transdermal moisture evaporation amount and skin roughness, the skin elasticity increase and the wrinkle depth decrease, the skin aging, the skin damage or skin elasticity decrease, the prevention and improvement of wrinkles and the skin whitening effect.
  • the milk cream yeast fermentation broth obtained in Example 4 was contained After the emulsion prepared by the method shown in [Table 1] of Example 5 was applied, changes in moisture content of the skin surface before and after the test were measured. As a result, it was confirmed that the moisture content of the skin surface of the milk cream yeast fermentation broth was increased as shown in Tables 27 and 28 below.
  • the emulsion was applied in the same manner as in the above ⁇ 5-1>, and then the change in oil retention amount on the skin surface before and after the test was measured. As a result, as shown in Tables 29 and 30, it was confirmed that the milk cream yeast fermentation broth yeast increased the oil content of the skin surface.
  • Lactobacillus lambosus B2 used as a control group as the present invention of the present inventors but also the lactobacillus lambsos milk cream yeast fermentation broth (P1) and the lactobacillus lambosus milk cream MRS fermentation broth (P2)
  • the fermentation liquids have an increased moisture retention and oil retention on the facial skin and neck skin surface, a decrease in the melanin index of the skin surface and an improvement in skin tone, whitening effects such as stain, dullness and senile black spots are improved or improved, And decreased skin roughness, increased skin elasticity, and reduced wrinkle depth, thereby contributing to skin aging, skin damage or skin elasticity reduction, prevention and improvement of wrinkles, and skin whitening effect.
  • the skin test for aging obtained by the method of ⁇ Experimental Example 1-1> was conducted for the oral Lactobacillus lambosus live cell extract (R1), the germ capsule (R2) and the mixed germ capsule (R3) The subjects were given 1 capsule / day of water each time, and the change of moisture content of the skin surface before and after the test was measured.
  • a lactobacillus lanosus oil-in-water cream emulsion prepared by the method described in [Table 1] of Example 5 was applied (B2), and the skin before and after the test The change in moisture content of the surface was measured. As a result, it was confirmed that the water holding amount was increased as shown in Tables 39 and 40 below.
  • rhamnosus ive cells
  • R2 ate oral L. rhamnosus 5033, dead cells
  • R3, ate oral L. rhamnosus 5033 , mixed cells
  • N the number of people in group.
  • the moisture content and oil content of the facial skin and neck skin surface are increased, the melanin index of the skin surface is reduced and the skin tone is improved, Skin aging, skin elasticity, and wrinkles by exhibiting a very high rate of change in whiteness, improvement in skin elasticity, decrease in wrinkle depth, and the like. And to contribute to skin whitening effect.
  • the lotion prepared by the method shown in [Table 2] of ⁇ Example 5> was applied to the atopic dermatitis lesion site using the lactic acid bacteria fermented with various lactic acid bacteria obtained in ⁇ Example 1>, and skin moisture And the change of the holding amount was measured.
  • visual evaluation of changes in atopic dermatitis lesions was carried out before and after the test.
  • Table 51 it was confirmed that the milk cream fermentation liquid using various lactic acid bacteria strains increased the skin moisture content of the atopic dermatitis lesion area.
  • the lotion was applied to lesions of atopic dermatitis lesions in the same manner as in the above ⁇ 7-1>, and the change of the acidity of the skin surface before and after the test was measured. As a result, as shown in Table 52, it was confirmed that the milk cream fermentation liquid using various lactic acid bacteria strains decreased the skin pH of lesions of atopic dermatitis.
  • the lotion was applied to the atopic dermatitis lesion area in the same manner as in the above ⁇ 7-1>, and then the change of the transdermal moisture evaporation amount before and after the test was measured. As a result, as shown in Table 53 below, it was confirmed that the milk cream fermentation liquid using various lactic acid bacteria strains reduced the amount of transdermal water evaporation at the lesions of atopic dermatitis.
  • Lactobacillus laminocus B2 used as a control group but also various lactic acid bacteria such as Lactococcus lactis (K1), Lactobacillus franarium (K2), Lactobacillus paracasei (K3) (K4), Lactobacillus delbruecki (K5), Lactobacillus luteri (K6), Lactobacillus salivarius (K7), Lactobacillus fermentum (K8), Lactobacillus acidophilus (K9)
  • K1 Lactococcus lactis
  • K2 Lactobacillus francis cyclarium
  • K3 Lactobacillus paracasei
  • Lactobacillus delbruecki K5
  • Lactobacillus luteri K6
  • Lactobacillus salivarius K7
  • Lactobacillus fermentum K8
  • Lactobacillus acidophilus K9
  • Each fermented milk cream fermentation solution increases the moisture content of the skin, decreases the pH value of the
  • the lotion prepared by the method shown in [Table 2] of Example 5 was applied to the atopic dermatitis lesion site with the intermittently sterilized or reduced-pressure concentrated milk cream fermentation liquid and the milk cream fermentation mixture obtained in Example 2 above
  • the changes in skin moisture content before and after the test were measured.
  • visual evaluation of changes in atopic dermatitis lesions was carried out before and after the test. As a result, it was confirmed that the skin moisture content of the atopic dermatitis lesion area was increased as shown in Table 54 below.
  • the lotion was applied to lesions of atopic dermatitis lesions in the same manner as in the above ⁇ 8-1>, and the change of the acidity of the skin surface before and after the test was measured. As a result, it was confirmed that the skin pH of atopic dermatitis lesions was reduced as shown in Table 55 below.
  • the lotion was applied to the atopic dermatitis lesion area in the same manner as in ⁇ 8-1>, and then the change of the transdermal moisture evaporation amount before and after the test was measured. As a result, it was confirmed that the amount of transdermal water evaporation in the atopic dermatitis lesion site was reduced as shown in Table 56 below.
  • Lactobacillus lambosus (B2) used as a control group as the present invention of the present inventors but also a fermentation broth (K11) of Lactobacillus laminosus milk cream which is intermittently sterilized (Tyndallization) or a lactobacillus lambsos milk cream fermentation solution of 90% ,
  • a mixed fermentation broth (K12) containing 10% by weight of a 10-fold fermentation concentrate of Lactobacillus laminocus a mixed fermentation broth (K13) containing 80% by weight of a fermentation broth of a lactobacillus lan- nomosus milk cream and 20%
  • 25% by weight of the lacto-koccus lactis milk cream fermentation liquid arbitrarily selected for 25% by weight of the lactobacillus laminosus milk cream fermentation liquid, 25% by weight of the lactobacillus gasseri oil cream fermentation broth selected from the lotions containing the fermented milk cream fermented with various lactic acid bacteria (K16) mixed with 25% by weight of Lactobacillus delbrueckii cream fermentation broth (K16) is a lesion of atopic dermatitis due to increase of skin moisture content of atopic dermatitis lesion, decrease of skin pH value and decrease of transdermal moisture evaporation Expression, but also contributes to the suppression of lesion and the inhibition of recurrence. Also, as shown in FIG. 4, it was visually confirmed that the atopic dermatitis lesion was remarkably alleviated.
  • Example 5 The lotion prepared by the method shown in [Table 2] of Example 5 with various milk cream yeast fermentation broth obtained in Example 4 was applied to the atopic dermatitis lesion, Change was measured. As a result, it was confirmed that a variety of milk cream yeast fermentation broth as shown in Table 57 is excellent in increasing the skin moisture content in atopic dermatitis lesion sites.
  • Lactococcus lactis (K1) which was tested in Experimental Example 7, as well as Lactobacillus laminocus (B2) used as a control group as an existing invention of the present inventors, (M2), Lactococcus lactis milk cream yeast fermentation broth (M3), Lactobacillus lactis milk yeast fermentation broth (M3), Lactobacillus lactis milk yeast fermentation broth Lactobacillus lactis milk cream MRS fermentation liquid (M4), Lactobacillus plantarum milk cream yeast fermentation liquid (M5), Lactobacillus plantarum milk cream MRS fermentation liquid (M6) inhibited the expression of lesions of atopic dermatitis, These various milk cream dairy fermentation liquids increased the skin moisture content, decreased the skin pH value, decreased the transdermal water evaporation amount On, it was confirmed that in addition to inhibiting the expression of atopic dermatitis lesions, which greatly contributes to the excellent inhibitory effects of mitigation and recurrence of the lesion.
  • Lactobacillus laminosus oil cream lotion (B2) prepared by the method shown in [Table 2] of ⁇ Example 5> was administered twice to the atopic dermatitis lesion site twice a day while taking oral lactobacillus lambsus mixed bacterial capsules After application (H4), changes in skin moisture content before and after the test were measured.
  • rhamnosus 5033 H1, ate oral L. rhamnosus 5033 , live cells; H2, ate oral L. rhamnosus 5033, dead cells; H3, ate oral L. rhamnosus 5033 , mixed cells; H4, ate oral L. rhamnosus 5033, mixed cells and applied test lotion made from fermented milk cream with KCTC 5033 on skin.
  • Lactobacillus lambusosus (B2) used as a control group as the conventional invention of the present inventors but also a case (H1) in which an oral Lactobacillus rhamnosus live cell capsule was taken (H2) But also contributes to the effect of suppressing the lesion and inhibiting the recurrence.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une composition cosmétique comprenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques et, plus précisément, une composition pour : la prévention et la réduction du vieillissement cutané, ce paramètre étant couramment utilisé comme mesure représentative de l'effet anti-âge ; la prévention et l'atténuation des lésions cutanées ; la prévention et l'atténuation de la dégradation de l'élasticité de la peau, et des rides ; le blanchiment de la peau ; et la prévention ou l'atténuation de la dermatite atopique, ainsi que diverses applications de ladite composition. La crème de lait liquide fermentée par Lactococcus spp., Lactobacillus spp. ou Bifidobacterium spp. de la présente invention a pour effets de prévenir et d'atténuer le vieillissement cutané en réduisant les aspérités de la peau, en améliorant la souplesse de la peau, en augmentant l'élasticité de la peau et en atténuant les rides cutanées. De plus, la crème de lait liquide fermentée empêche et atténue les lésions cutanées en augmentant la rétention d'eau et de lipides au niveau de la surface cutanée et en faisant baisser la perte d'eau transépidermique. De plus, la crème de lait liquide fermentée conserve un effet de renforcement du blanchiment de la peau en atténuant les taches de rousseur, les imperfections et les taches de vieillesse, en faisant baisser l'indice de mélanine au niveau de la surface de la peau et en améliorant le teint, et a des effets de prévention ou de soulagement des symptômes de la dermatite atopique par augmentation de la rétention d'eau dans les lésions associées à la dermatite atopique, de stabilisation de la peau par réduction de la valeur du pH de la peau et de réduction de la perte d'eau transépidermique, induisant une plus grande souplesse de la peau. Par conséquent, cette crème de lait liquide fermentée peut être utilement utilisée dans le domaine des produits cosmétiques ou des aliments en lien avec les effets précédents.
PCT/KR2018/011110 2017-09-22 2018-09-20 Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques WO2019059668A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020170122632A KR101975671B1 (ko) 2017-09-22 2017-09-22 유산균의 유크림 발효액을 유효성분으로 포함하는 아토피 피부염의 예방 및 개선용 조성물
KR10-2017-0122632 2017-09-22
KR1020170142730A KR102015180B1 (ko) 2017-10-30 2017-10-30 유산균의 유크림 발효액을 유효성분으로 포함하는 화장료 조성물
KR10-2017-0142730 2017-10-30

Publications (2)

Publication Number Publication Date
WO2019059668A2 true WO2019059668A2 (fr) 2019-03-28
WO2019059668A3 WO2019059668A3 (fr) 2019-05-31

Family

ID=65810478

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/011110 WO2019059668A2 (fr) 2017-09-22 2018-09-20 Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques

Country Status (1)

Country Link
WO (1) WO2019059668A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113197784A (zh) * 2021-04-13 2021-08-03 北京源天彩生物科技有限公司 一种含生物发酵制备的黑色素前体物的染发产品的制备方法与应用
CN113278544A (zh) * 2021-05-11 2021-08-20 君维安(武汉)生命科技有限公司 一种副干酪乳杆菌、其发酵液的制备方法、应用及产品
CN114452241A (zh) * 2022-03-16 2022-05-10 渤海大学 一种益生菌小分子面膜及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0605949D0 (en) * 2006-03-24 2006-05-03 Quest Int Serv Bv Milk product for skin treatment
KR101104196B1 (ko) * 2009-11-25 2012-01-09 중앙대학교 산학협력단 발효유 제조에 유용한 신균주 락토바실러스 카세이 ｃｕ２６０４ 및 이를 포함하는 발효유
KR101501210B1 (ko) * 2012-07-31 2015-03-11 중앙대학교 산학협력단 항-염증 활성이 우수한 신규 균주
KR20160110829A (ko) * 2015-03-13 2016-09-22 주식회사한국야쿠르트 피부 미백 효능을 가지는 락토바실러스 파라카제이 hy7301 우유 발효물 및 이를 유효성분으로 함유하는 제품
KR102061387B1 (ko) * 2016-03-25 2019-12-31 건국대학교 산학협력단 락토바실러스 람노서스 배양액 및 공액리놀레산을 유효성분으로 함유하는 아토피 피부염 개선용 화장료 조성물

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113197784A (zh) * 2021-04-13 2021-08-03 北京源天彩生物科技有限公司 一种含生物发酵制备的黑色素前体物的染发产品的制备方法与应用
CN113278544A (zh) * 2021-05-11 2021-08-20 君维安(武汉)生命科技有限公司 一种副干酪乳杆菌、其发酵液的制备方法、应用及产品
CN113278544B (zh) * 2021-05-11 2022-03-01 君维安(武汉)生命科技有限公司 一种副干酪乳杆菌、其发酵液的制备方法、应用及产品
CN114452241A (zh) * 2022-03-16 2022-05-10 渤海大学 一种益生菌小分子面膜及其制备方法

Also Published As

Publication number Publication date
WO2019059668A3 (fr) 2019-05-31

Similar Documents

Publication Publication Date Title
WO2018174669A1 (fr) Produit fermenté de colostrum et composition cosmétique l'utilisant
WO2019059668A2 (fr) Composition pour la peau contenant, en tant que principe actif, de la crème de lait liquide fermentée par des bactéries lactiques
WO2016013709A1 (fr) Composition pour l'amélioration de la peau, contenant du concentré de grenade en tant que principe actif
WO2021261975A1 (fr) Extrait végétal comprenant du collagène végétal et de la mucine végétale, et son procédé de préparation
WO2017222317A1 (fr) Composition ayant pour effet d'améliorer l'hydratation de la peau, d'éliminer la kératine cutanée, d'améliorer l'élasticité de la peau, d'inhiber l'érythème, d'atténuer les rides cutanées ou d'atténuer le photovieillissement cutané, contenant une ionone ou un sel de celle-ci comme ingrédient actif
WO2022080839A1 (fr) Composition antioxydante, anti-inflammatoire et de blanchiment comprenant des bactéries d'acide lactique dérivées de la peau
WO2022131847A1 (fr) Procédé de production en continu pour améliorer la survie à la lyophilisation, la tolérance à la chaleur, la stabilité en rayon et la stabilité digestive des probiotiques en utilisant une technique d'encapsulation matricielle spontanée
KR20190048152A (ko) 유산균의 유크림 발효액을 유효성분으로 포함하는 화장료 조성물
WO2017146414A1 (fr) Composition pour l'hydratation de la peau et atténuation des rides cutanées, contenant du α-terpinéol en tant que substance active
WO2017164681A1 (fr) Composition cosmétique destinée à soulager la dermatite atopique, contenant, en tant que principes actifs, une solution de culture de lactobacillus rhamnosus, ou une solution de culture de lactobacillus rhamnosus et de l'acide linoléique conjugué
WO2018186640A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer son élasticité, l'exfolier, inhiber l'érythème ou réduire le photovieillissement cutané, contenant de l'ocimène ou un sel de celui-ci à titre de substance active
WO2018004141A1 (fr) Composition ayant pour effet d'améliorer l'hydratation de la peau, d'exfolier la peau, d'améliorer l'élasticité de la peau, d'inhiber l'érythème, d'atténuer les rides cutanées ou d'atténuer le photovieillissement cutané, contenant, en tant qu'ingrédient actif, un ou plusieurs composés choisis dans le groupe constitué du cimène, de l'acide béhénique, du 2-méthoxynaphtalène, du thymol et de leurs sels
WO2018117725A1 (fr) Composition pour prévenir la chute des cheveux et favoriser la pousse des cheveux, comprenant des produits animaux marins fermentés
WO2017213346A1 (fr) Composition contenant de la diosmine ou son sel comme ingrédient actif et présentant un effet d'amélioration de l'hydratation de la peau, d'exfoliation de la peau, de renforcement de l'élasticité de la peau, d'inhibition de l'érythème, de réduction des rides de la peau, ou de retardement du photovieillissement de la peau
WO2010143879A2 (fr) Composition cosmétique destinée à éliminer les rides, contenant un extrait d'ormeau en tant que principe actif
WO2018186641A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer son élasticité, l'exfolier, inhiber l'érythème ou réduire le photovieillissement de la peau, contenant du pipéronal ou l'un de ses sels à titre de principe actif
WO2018186644A1 (fr) Composition pour réduire les rides cutanées, hydrater la peau, améliorer l'élasticité de la peau, exfolier la peau, inhiber un érythème ou réduire le photovieillissement de la peau, contenant de la jasmone ou un sel de celle-ci en tant que substance active
WO2018186643A1 (fr) Composition pour réduire les rides de la peau, hydrater la peau, améliorer l'élasticité de la peau, exfolier la peau, inhiber l'érythème ou réduire le photo-vieillissement de la peau, contenant de la cinchonine ou un sel de celle-ci en tant que principe actif
WO2017142265A1 (fr) Composition contenant de l'acide adipique utilisé comme principe actif pour l'atténuation des rides de la peau et l'amélioration de l'élasticité de la peau
WO2018128479A1 (fr) Composition pour la prévention ou le traitement de maladies musculaires, comprenant comme principe actif de l'acide subérique ou un sel pharmaceutiquement acceptable de celui-ci
WO2020085826A1 (fr) Composition pour soulager une irritation de la peau induite par des facteurs de pollution environnementaux ou pour la protection de la peau, contenant comme principe actif un extrait de noix de muscade ou du macelignane
WO2016117762A1 (fr) Composition contenant un extrait de groseille à maquereau ou du glutathion
WO2022086218A1 (fr) Procédé de construction d'un modèle de dermatite atopique à l'aide d'un organoïde cutané dérivé de cellules souches pluripotentes
WO2015167240A1 (fr) Composition contenant un extrait de scutellaria alpina
WO2021002642A1 (fr) Composition pour prévenir ou traiter la polyarthrite rhumatoïde, comprenant du venin de serpent

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18858591

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18858591

Country of ref document: EP

Kind code of ref document: A2