WO2021206227A1 - Cosmetic composition comprising quercus acuta thunb fruit extract for preventing and alleviating ultraviolet light-caused skin aging - Google Patents
Cosmetic composition comprising quercus acuta thunb fruit extract for preventing and alleviating ultraviolet light-caused skin aging Download PDFInfo
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
Definitions
- the present invention relates to a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays comprising a hot water extract of Quercus acuta Thunb fruit as an active ingredient. More specifically, the cosmetic composition for preventing and improving skin aging due to ultraviolet rays according to the present invention has excellent effect of inhibiting collagenase activity and increasing collagen synthesis when UV-B treatment of HaCaT cells, which is a human dermal keratinocyte line, is excellent. It relates to a cosmetic composition for preventing and improving skin aging that can be safely used without toxicity and side effects.
- UV A 320-400 nm
- ultraviolet B 280-320 nm
- ultraviolet C (190-280 nm).
- ultraviolet C has no significant effect on natural photochemical reactions because it is absorbed, scattered and filtered by the ozone layer in the upper atmosphere.
- UV A ultraviolet A
- UV B ultraviolet B
- UV A is primarily emitted from the sun, but is also emitted by artificial lamps, etc., and penetrates deeply into the epidermis and dermis layer of the skin. Therefore, ultraviolet A is currently regarded as the factor that most affects the skin.
- UV B irradiation is more dangerous than UV A. That is, UVB has a shorter wavelength than UVA and is known not to penetrate deep into the skin, but has much stronger energy than UVA, and is known as a wavelength that promotes photoaging of the skin while generating sharp erythema on the skin surface.
- UV B causes photoaging by damaging DNA and connective tissue.
- the period when UV rays are strongest is from April to September, and among them, the peak period is from May to June.
- Skin aging caused by continuous exposure to such ultraviolet rays is called photoaging, and the most prominent symptoms of photoaging are fine wrinkles, spots, and pigmentation, etc., and are proportional to the time and intensity of exposure to the sun.
- matrix metal which is an enzyme that breaks down collagen or gelatin, which are components of the skin's extracellular matrix (ECM), as reactive oxygen species formed by UV light send a signal to the cell signaling system.
- ECM extracellular matrix
- MMP matrix metalloproteinase
- Quercus acuta THUNB a natural resource in Korea, is an evergreen broad-leaved arboreous tree of the genus Oak, growing in sunny foothills and valleys of 170-500 m above sea level in Jeollanam-do, Gyeongsangbuk-do, and Jeju-do. It is a tree that grows to a height of 20m and a diameter of 60cm or more, and the young branches are densely covered with brown hairs. The leaves are alternate phyllotaxis, long oval or long ovate, 7-13 cm long, and the edges are flat. The flower is a single flower, blooms in May, and the fruit is 2cm long and ripens in October of the next year. Because of the red color of the wood, it got its name from the red thorn tree.
- An object of the present invention is to provide a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays that can be used.
- a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays which contains, as an active ingredient, as an active ingredient, a hot-water extract from red bramble fruits, which can be safely used without toxicity and side effects, using the natural raw material, red thorn fruit, for the purpose of utilizing Korea's natural plant resources. do.
- the present invention provides a cosmetic composition for preventing and improving skin aging due to ultraviolet rays, characterized in that it contains a hot water extract of Quercus acuta Thunb fruit.
- the red bramble of the present invention is extracted from one or more selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, ethyl acetate, acetone, ether, chloroform, benzene, hexane, dichloromethane, and mixtures thereof.
- a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing a red bramble extract extracted with a solvent.
- the cosmetic composition for preventing and improving skin aging due to ultraviolet rays is an external preparation for skin formulation containing 0.01 to 50.0 dry weight % of the cosmetic composition for preventing and improving skin aging by ultraviolet rays containing the red bramble extract of the present invention.
- the cosmetic composition for preventing and improving skin aging by ultraviolet rays of the present invention is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, sun cream , foundation, essence, nutritional essence, pack, soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser can
- composition containing the extract of the present invention when HaCaT cells, a human skin keratinocyte line, were treated with UV-B as a control group, it was confirmed that the collagenase activity was increased compared to the activity of the normal group, but UV-B and red thorns
- the activity of collagenase was decreased as the concentration of the red bramble extract increased, so the effect of inhibiting the activity of collagenase was excellent, and it increased collagen synthesis without toxicity and side effects It was confirmed that it can be safely used as a cosmetic composition for preventing and improving skin aging due to ultraviolet rays.
- 1 is a photograph of the fruit, which is a raw material of the red bramble used in the present invention.
- Figure 2 is a schematic diagram showing the process of preparing a Quercus acuta Thunb extract and fractions using a red bramble raw material as a solvent.
- Figure 3 is a photograph of the powder of the red bramble extract.
- Figure 4 shows the cytotoxicity test results of the hot water extract of Red bramble.
- Figure 5 shows the collagen synthesis enhancing effect of red bramble hot water extract.
- Figure 6 shows the collagenase activity inhibitory effect of the hot water extract of Red bramble.
- FIG. 7 shows a cosmetic lotion formulation for preventing and improving skin aging by ultraviolet rays prepared by using the red bramble extract and extract of the present invention.
- FIG. 1 shows a photograph of a fruit, which is a raw material of brambles used in the present invention
- FIG. 2 is a schematic diagram showing a process for preparing extracts and fractions from Quercus acuta Thunb using a bramble as a raw material as a solvent.
- a dry raw material supplied through a commercial pharmaceutical store was used as the raw material of the red bramble used in the present invention.
- Quercus acuta Thunb extract is prepared by soluble in any one selected from water or alcohols having 1 to 5 carbon atoms and a mixed solvent thereof. 7.5L distilled water was added to 500 g of dried red bramble, and heated at 100° C. for 3 hours, followed by hot water extraction. The extracted hot water extract was concentrated under reduced pressure and freeze-dried to obtain 91.2 g of a hot water freeze-dried product of Quercus acuta Thunb.
- Figure 3 shows a photograph of the powder of the red bramble extract.
- the hexane-soluble fraction, the chloroform-soluble fraction, the ethyl acetate-soluble fraction and the butanol-soluble fraction were concentrated under reduced pressure from the hot-water extract of Red bramble, and then freeze-dried to obtain a hexane fraction, a chloroform fraction, an ethyl acetate fraction, and a butanol fraction, which were used as samples.
- FIG. 4 shows the cytotoxicity test results of the red bramble extract.
- MTT assay was performed to measure the cell viability of human skin keratinocytes (HaCaT) to the hot water extract of Red bramble.
- HaCaT human skin keratinocytes
- DMEM medium containing MTT at 1 mg/ml was treated with 1 ml per well, and cultured for 4 hours in a humidified CO2 incubator at 37°C.
- the tetrazolium bromide salt was removed, and 200 ⁇ l of DMSO was dispensed to dissolve the formazan crystal generated in each well, and the absorbance was measured at a wavelength of 570 nm in a microplate reader to determine the cell viability. It was confirmed.
- Figure 5 shows the collagen synthesis promoting effect of the hot water extract of brambles.
- a Procollagen Type I C-Peptide Enzyme Immuno Assay experiment was performed to evaluate the collagen synthesis promoting effect of the hot water extract of Red bramble.
- HaCaT cells which are human dermal keratinocytes , were inoculated in a 24-well microplate so as to become 5x10 4 cells per well, and cultured in DMEM medium at 37° C. until they grow to 70-80%. Thereafter, it was replaced with serum-free DMEM medium and treated with UV-B 30mJ/cm 2 to induce collagen degradation. 10, 50, 100, and 200 ⁇ g/ml of the red bramble extract were treated by concentration and then further cultured in a serum-free medium for 48 hours. After incubation, the amount of newly synthesized collagen was measured by collecting the supernatant from each well and measuring the amount of procollagen type I C-peptide (PICP) using a kit (Takara, Japan).
- PICP procollagen type I C-peptide
- FIG 6 shows the collagenase (MMP-1) activity inhibitory effect of the hot water extract of Rhododendron.
- An MMP-1 activity inhibition experiment was performed to evaluate the collagen degradation inhibitory ability of the hot water extract of Red bramble.
- HaCaT cells which are human dermal keratinocytes , were inoculated in a 96-well microplate so that 1X 10 4 cells per well were inoculated, and cultured in DMEM medium at 37° C. until 70-80% growth. Thereafter, it was replaced with serum-free DMEM medium and treated with UV-B 30mJ/cm 2 to induce an increase in collagenase activity.
- the experimental group was treated with 10, 50, 100, and 200 ⁇ g/ml of red bramble, and then further cultured in a serum-free medium for 48 hours. After incubation, the supernatant of each well was collected and the activity of total MMP-1 enzyme was confirmed at 450 nm using an MMP-1 assay kit (R&D systems, USA).
- the red bramble extract of the present invention was effective in preventing and improving skin aging caused by UV rays by participating in the inhibition of collagenase activity increased by UV-B and inhibiting the promotion of collagen degradation. In addition, it was found that it exhibits an excellent collagen synthesis enhancing effect.
- Example 5 Preparation of cosmetics for preventing and improving skin aging due to ultraviolet rays using a red bramble extract
- FIG. 7 shows a cosmetic lotion formulation for preventing and improving skin aging by ultraviolet rays prepared by using the red bramble extract and extract of the present invention.
- Example 2 To 100 ml of purified water, 10 mg of the hot water extract prepared in Example 1, 1 mg of triethanolamine, 0.5 mg of aloe vera extract, 0.5 mg of salicylic acid, 0.5 mg of allantoin, 0.5 mg of jojoba oil, and 0.5 mg of tocopherol as a pH adjuster were added. After heating to 50° C., a lotion was prepared by emulsifying it by mixing it with an oily layer in which 20 ml of shea butter as a vegetable oil and 20 ml of cetyl alcohol as an emulsifier were mixed.
- Cosmetics for external application for skin are skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, sun cream, foundation, essence, nourishing essence, pack. , soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.
- the present invention increases collagen synthesis by providing a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays comprising an extract of Quercus acuta Thunb as an active ingredient, and has excellent effect of inhibiting collagenase activity, toxicity and side effects It has industrial applicability because it can produce cosmetic products for skin aging prevention and improvement that can be safely used without the need for production and profit in related industries.
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- Cosmetics (AREA)
Abstract
The present invention relates to a cosmetic composition comprising a Quercus acuta Thunb extract as an active ingredient for preventing and alleviating ultraviolet light-caused skin aging. It was observed that when treated with the control group UVB, HaCaT cells, which are a human epidermal keratinocyte line, increased in collagenase activity, compared to a normal group whereas, for a test group treated with UVB and a Quercus acuta Thunb extract together, collagenase activity decreased as the concentration of the Quercus acuta Thunb extract increased. Thus, the cosmetic composition for prevention and alleviation of ultraviolet light-caused skin aging according to the present invention has an excellent inhibitory effect on collagenase activity and can be used safely without toxicity and adverse effects.
Description
본 발명은 붉가시나무 (Quercus acuta Thunb) 열매 열수추출물을 유효성분으로 포함하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물에 관한 것이다. 더욱 상세하게는 본 발명에 따른 자외선에 의한 피부노화방지 및 개선용 화장료 조성물은 사람 피부각질세포주인 HaCaT세포에 UV-B를 처리하는 경우, 콜라게나아제의 활성 저해 효과가 우수하며 콜라겐 합성을 증가시켜 독성 및 부작용 없이 안전하게 사용될 수 있는 피부노화방지 및 개선용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays comprising a hot water extract of Quercus acuta Thunb fruit as an active ingredient. More specifically, the cosmetic composition for preventing and improving skin aging due to ultraviolet rays according to the present invention has excellent effect of inhibiting collagenase activity and increasing collagen synthesis when UV-B treatment of HaCaT cells, which is a human dermal keratinocyte line, is excellent. It relates to a cosmetic composition for preventing and improving skin aging that can be safely used without toxicity and side effects.
햇빛에는 가시광선, 적외선, 그리고 자외선이 존재하는데 자외선(Ultraviolet)은 그 파장에 따라 자외선 A(320-400nm), 자외선 B(280-320nm) 그리고 자외선 C(190-280nm) 등 세 가지로 나누어진다. 일반적으로 자외선 C는 대기권의 상층부에 있는 오존층에서 흡수, 산란되어 여과되므로 자연적 광화학 반응에 별다른 영향을 미치지 않는다. Visible light, infrared light, and ultraviolet light exist in sunlight. Ultraviolet is divided into three types according to its wavelength: ultraviolet A (320-400 nm), ultraviolet B (280-320 nm), and ultraviolet C (190-280 nm). . In general, ultraviolet C has no significant effect on natural photochemical reactions because it is absorbed, scattered and filtered by the ozone layer in the upper atmosphere.
인체에 주로 영향을 주는 자외선은 자외선 A(Ultraviolet-A)와 자외선 B(Ultraviolet-B)이다. 자외선 A는 일차적으로는 태양으로부터 방출되나 인공 램프 등에 의해서도 방출되며 피부의 표피와 진피층에 깊숙하게 투과한다. 따라서 자외선 A는 현재 피부에 가장 많은 영향을 주는 요소로서 주시되고 있다. 또한 자외선 B 조사는 자외선 A 보다 더 위험하다. 즉 자외선 B는 자외선 A 보다 파장이 짧아서 피부 깊숙이 침투하지는 못한다고 알려져 있으나 자외선A 보다 훨씬 강한 에너지를 가지고 있어서 피부 표면에 예리한 홍반을 발생시키는 한편 피부의 광노화를 촉진시키는 파장으로 알려져 있다.The ultraviolet rays that mainly affect the human body are ultraviolet A (Ultraviolet-A) and ultraviolet B (Ultraviolet-B). Ultraviolet A is primarily emitted from the sun, but is also emitted by artificial lamps, etc., and penetrates deeply into the epidermis and dermis layer of the skin. Therefore, ultraviolet A is currently regarded as the factor that most affects the skin. Also, UV B irradiation is more dangerous than UV A. That is, UVB has a shorter wavelength than UVA and is known not to penetrate deep into the skin, but has much stronger energy than UVA, and is known as a wavelength that promotes photoaging of the skin while generating sharp erythema on the skin surface.
자외선 B는 DNA와 결체조직에 손상을 주어 광노화(photoaging)를 일으킨다. 자외선이 강한 시기는 4월부터 9월이며, 그 중에서도 5~6월이 최고조에 이른다. 이러한 자외선에 지속적으로 노출되어 생긴 피부노화를 광노화(Photoaging)라 하는데, 상기 광노화의 가장 두드러진 증상은 미세주름, 반점, 및 색소침착 등이며, 태양에 노출된 시간과 강도에 비례한다.Ultraviolet B causes photoaging by damaging DNA and connective tissue. The period when UV rays are strongest is from April to September, and among them, the peak period is from May to June. Skin aging caused by continuous exposure to such ultraviolet rays is called photoaging, and the most prominent symptoms of photoaging are fine wrinkles, spots, and pigmentation, etc., and are proportional to the time and intensity of exposure to the sun.
자외선에 의한 피부 손상 원인 중 하나는 자외선에 의해 형성된 활성 산소종이 세포 신호체계에 신호를 보내어 결국 피부의 세포간물질 (extracellular matrix, ECM)의 성분인 콜라겐이나 젤라틴 등을 분해하는 효소인 메트릭스 메탈로프로티나제 (matrix metalloproteinase, MMP)를 생성하여 콜라겐이나 젤라틴을 파괴함으로서, 피부에 주름이 형성되게 하는 것이다. 만성적인 자외선의 영향을 상징적으로 나타내는 것으로 농부 혹은 어부의 피부를 들 수 있는데, 그 특징은 외형적으로는 피부의 색이 검고 촉감이 뻣뻣하며 깊은 주름이 생긴다. 이 상태가 더욱 심해지면 피부암을 유발하는데 이러한 피부의 변화를 자연노화와 구분하여 광노화라고 한다.One of the causes of skin damage caused by UV rays is matrix metal, which is an enzyme that breaks down collagen or gelatin, which are components of the skin's extracellular matrix (ECM), as reactive oxygen species formed by UV light send a signal to the cell signaling system. By generating proteinase (matrix metalloproteinase, MMP) and destroying collagen or gelatin, it causes wrinkles to form on the skin. The skin of a farmer or a fisherman is a symbol of the effects of chronic ultraviolet rays, and the characteristics of the skin are black in appearance, stiff to the touch, and deep wrinkles occur. When this condition becomes more severe, it causes skin cancer, and this change in skin is called photoaging to distinguish it from natural aging.
한편, 국내 천연자원인 붉가시나무(Quercus acuta THUNB. )는 전라남도, 경상남북도 및 제주도의 표고 170~500m의 양지바른 산기슭과 계곡에서 많이 자라는 참나무과 참나무속의 상록활엽교목이다. 높이 20m, 지름 60㎝ 이상으로 자라는 나무로 어린 가지에는 갈색 털이 밀포한다. 잎은 어긋나며 긴 타원형 또는 긴 난형으로 길이 7∼13㎝이고 가장자리가 밋밋하다. 꽃은 1가화로서 5월에 피고 열매는 길이 2㎝로서 다음해 10월에 익는다. 목재의 빛깔이 붉은빛을 띄기 때문에 붉가시나무라는 이름이 붙었다. On the other hand, Quercus acuta THUNB., a natural resource in Korea, is an evergreen broad-leaved arboreous tree of the genus Oak, growing in sunny foothills and valleys of 170-500 m above sea level in Jeollanam-do, Gyeongsangbuk-do, and Jeju-do. It is a tree that grows to a height of 20m and a diameter of 60cm or more, and the young branches are densely covered with brown hairs. The leaves are alternate phyllotaxis, long oval or long ovate, 7-13 cm long, and the edges are flat. The flower is a single flower, blooms in May, and the fruit is 2cm long and ripens in October of the next year. Because of the red color of the wood, it got its name from the red thorn tree.
본 발명에서는 우리나라의 천연 식물 원료인 붉가시나무를 이용한 조성물로 피부각질세포주인 HaCaT세포에 UV-B를 처리하는 경우, 콜라게나아제의 활성 저해 효과가 우수하며 콜라겐 합성을 증가시켜 독성 및 부작용 없이 안전하게 사용될 수 있는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공하고자 한다.In the present invention, when UV-B is treated to HaCaT cells, which are keratinocytes of the skin, with a composition using red bramble, a natural plant raw material in Korea, the effect of inhibiting collagenase activity is excellent, and collagen synthesis is increased to safely and without toxicity and side effects. An object of the present invention is to provide a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays that can be used.
우리나라의 천연 식물자원을 활용할 목적으로 천연원료인 붉가시나무 열매를 이용하여 독성 및 부작용 없이 안전하게 사용가능한 붉가시나무 열매 열수추출물을 유효성분으로 포함하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공하고자 한다.To provide a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays, which contains, as an active ingredient, as an active ingredient, a hot-water extract from red bramble fruits, which can be safely used without toxicity and side effects, using the natural raw material, red thorn fruit, for the purpose of utilizing Korea's natural plant resources. do.
본 발명의 목적을 달성하기 위해, 본 발명은 붉가시나무 (Quercus acuta Thunb) 열매 열수추출물을 함유하는 것을 특징으로 하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공한다. In order to achieve the object of the present invention, the present invention provides a cosmetic composition for preventing and improving skin aging due to ultraviolet rays, characterized in that it contains a hot water extract of Quercus acuta Thunb fruit.
상기 과제를 해결하기 위하여 본 발명의 붉가시나무는 물, 탄소수 1 내지 5의 알코올, 에틸아세테이트, 아세톤, 에테르, 클로로포름, 벤젠, 헥산, 디클로로메탄 및 이의 혼합물로 이루어 진 군 중에서 선택된 1종 이상을 추출용매로 추출한 붉가시나무 추출물을 함유하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공한다.In order to solve the above problems, the red bramble of the present invention is extracted from one or more selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, ethyl acetate, acetone, ether, chloroform, benzene, hexane, dichloromethane, and mixtures thereof. Provided is a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing a red bramble extract extracted with a solvent.
또한, 상기 추출용매를 사용하여 추출한 붉가시나무 조추출물에 비극성용매로서 헥산, 클로로포름, 디클로메탄 및 에틸아세테이트 중 어느 하나를 분획용매로 사용하여 분획한 붉가시나무 추출물을 함유하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공한다.In addition, prevention of skin aging due to ultraviolet rays containing a bramble extract fractionated by using any one of hexane, chloroform, dichloromethane and ethyl acetate as a non-polar solvent in the crude extract of brambles extracted using the extraction solvent. And it provides a cosmetic composition for improvement.
본 발명의 붉가시나무 추출물을 함유하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 0.01 내지 50.0 건 중량% 함유하는 피부외용제 제형인 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공한다. 본 발명의 자외선에 의한 피부노화방지 및 개선용 화장료 조성물은 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스쳐로션, 영양로션, 맛사지크림, 영양크림, 모이스처크림, 핸드크림, 선크림, 파운데이션, 에센스, 영양에센스, 팩, 비누, 샴푸, 린스, 헤어트리트먼트, 화장용물티슈, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션 및 바디클렌저로 구성된 군으로부터 선택되는 어느 하나의 제형으로 이루어질 수 있다.It provides a cosmetic composition for preventing and improving skin aging due to ultraviolet rays, which is an external preparation for skin formulation containing 0.01 to 50.0 dry weight % of the cosmetic composition for preventing and improving skin aging by ultraviolet rays containing the red bramble extract of the present invention. The cosmetic composition for preventing and improving skin aging by ultraviolet rays of the present invention is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, sun cream , foundation, essence, nutritional essence, pack, soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser can
본 발명의 붉가시나무 추출물을 함유하는 조성물은 사람 피부각질세포주인 HaCaT세포에 대조군인 UV-B를 처리한 경우, 콜라게네이즈 활성이 정상군의 활성에 비해 증가했음을 확인하였으나, UV-B와 붉가시나무 추출물을 동시에 처리한 실험군의 경우, 붉가시나무 추출물의 농도가 증가함에 따라 콜라게네이즈의 활성이 감소되는 것을 확인하여 콜라게네이즈의 활성 저해 효과가 우수하며, 콜라겐 합성을 증가시켜 독성 및 부작용 없이 안전하게 자외선에 의한 피부노화방지 및 개선용 화장료 조성물로서 사용될 수 있는 것으로 확인하였다.In the case of the composition containing the extract of the present invention, when HaCaT cells, a human skin keratinocyte line, were treated with UV-B as a control group, it was confirmed that the collagenase activity was increased compared to the activity of the normal group, but UV-B and red thorns In the case of the experimental group treated with the tree extract at the same time, it was confirmed that the activity of collagenase was decreased as the concentration of the red bramble extract increased, so the effect of inhibiting the activity of collagenase was excellent, and it increased collagen synthesis without toxicity and side effects It was confirmed that it can be safely used as a cosmetic composition for preventing and improving skin aging due to ultraviolet rays.
도 1은 본 발명에서 사용된 붉가시나무의 원료인 열매의 사진이다. 1 is a photograph of the fruit, which is a raw material of the red bramble used in the present invention.
도 2는 붉가시나무 원료를 용매를 이용하여 붉가시나무 (Quercus acuta Thunb) 추출물 및 분획물을 제조하는 과정을 나타낸 모식도이다.Figure 2 is a schematic diagram showing the process of preparing a Quercus acuta Thunb extract and fractions using a red bramble raw material as a solvent.
도 3은 붉가시나무 추출물의 파우더 사진이다.Figure 3 is a photograph of the powder of the red bramble extract.
도 4는 붉가시나무 열수추출물의 세포 독성실험결과를 나타낸 것이다. Figure 4 shows the cytotoxicity test results of the hot water extract of Red bramble.
도 5은 붉가시나무 열수추출물의 콜라겐 합성 증진 효과를 나타낸 것이다.Figure 5 shows the collagen synthesis enhancing effect of red bramble hot water extract.
도 6은 붉가시나무 열수추출물의 콜라게네이즈 활성저해 효과를 나타낸 것이다.Figure 6 shows the collagenase activity inhibitory effect of the hot water extract of Red bramble.
도 7은 본 발명의 붉가시나무 원물 및 추출물을 이용하여 제조한 자외선에 의한 피부노화방지 및 개선용 화장품 로션 제형을 나타낸다.7 shows a cosmetic lotion formulation for preventing and improving skin aging by ultraviolet rays prepared by using the red bramble extract and extract of the present invention.
본 발명의 붉가시나무 열매추출물을 함유하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물과 관련한 구체적인 구성과 첨부한 도면을 참조하여 상세히 설명하면 다음과 같다.The detailed configuration of the cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing the red bramble fruit extract of the present invention will be described in detail with reference to the accompanying drawings.
도 1은 본 발명에서 사용된 붉가시나무의 원료인 열매의 사진을 나타내고, 도 2는 붉가시나무 원료를 용매를 이용하여 붉가시나무 (Quercus acuta Thunb) 추출물 및 분획물을 제조하는 과정을 나타낸 모식도이다. 본 발명에서 사용된 붉가시나무의 원료는 시중 약제상을 통해 공급받은 건조 원물을 사용하였다.1 shows a photograph of a fruit, which is a raw material of brambles used in the present invention, and FIG. 2 is a schematic diagram showing a process for preparing extracts and fractions from Quercus acuta Thunb using a bramble as a raw material as a solvent. As the raw material of the red bramble used in the present invention, a dry raw material supplied through a commercial pharmaceutical store was used.
실시예 1. 붉가시나무 (Quercus acuta Thunb) 추출물과 분획물 제조Example 1. Preparation of Quercus acuta Thunb extracts and fractions
붉가시나무 (Quercus acuta Thunb) 추출물은 물 또는 탄소수 1 내지 5의 알코올 및 이들의 혼합 용매 중에서 선택되는 어느 하나로부터 가용하여 제조한다. 건조된 붉가시나무 500g에 7.5L 증류수를 첨가하여 100℃에서 3시간동안 가열하여 열수 추출하였다. 상기 추출된 열수 추출물을 감압 농축 및 동결 건조하여 붉가시나무 (Quercus acuta Thunb) 열수 동결건조물 91.2g 얻었다. 도 3은 붉가시나무 추출물의 파우더 사진을 나타낸다.Quercus acuta Thunb extract is prepared by soluble in any one selected from water or alcohols having 1 to 5 carbon atoms and a mixed solvent thereof. 7.5L distilled water was added to 500 g of dried red bramble, and heated at 100° C. for 3 hours, followed by hot water extraction. The extracted hot water extract was concentrated under reduced pressure and freeze-dried to obtain 91.2 g of a hot water freeze-dried product of Quercus acuta Thunb. Figure 3 shows a photograph of the powder of the red bramble extract.
붉가시나무의 극성용매, 비극성용매 가용 분획물의 제조는 붉가시나무 열수 추출물을 증류수 1L에 완전히 용해시킨 후 분획 여두 깔대기에 넣고 헥산(Hexane) 1L를 첨가하여 water층과 hexane 층을 분리하였고 이와 같은 공정을 3번 반복하였다. 동일한 과정을 통해 클로로포름 (chloroform), 에틸아세테이트(ethyl acetate), 부탄올(butanol)을 순차적으로 가하여 각 분획물을 얻었고, 얻어진 각각의 분획물을 감압여과 장치로 여과하여 농축한 후 동결건조하여 용매를 완전히 제거한 뒤 본 실험에 사용하였다. Preparation of polar solvent and non-polar solvent soluble fractions of red bramble is After completely dissolving the hot water extract of Red thorn tree in 1L of distilled water, it was put into a funnel fractionation funnel, 1L of hexane was added to separate the water layer and the hexane layer, and this process was repeated 3 times. Through the same process, chloroform, ethyl acetate, and butanol were sequentially added to obtain each fraction, and each fraction obtained was filtered through a reduced pressure filtration device, concentrated, and then lyophilized to completely remove the solvent. It was then used in this experiment.
1.1. 헥산 가용성 분획 분리1.1. Separation of hexane-soluble fraction
붉가시나무 열수 추출물을 lL의 증류수에 완전히 용해시킨 후에 분획 여두 깔대기에 넣고 헥산 1L를 첨가하여 헥산 불용성층(수층)과 헥산가용성층을 분리하였다. 다시 헥산 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 헥산 불용성 분획 및 가용성 분획을 수집하였다. After completely dissolving the hot water extract of Red brambles in lL of distilled water, it was placed in a funnel separatory funnel, and 1L of hexane was added to separate the hexane-insoluble layer (water layer) and the hexane-soluble layer. Again, the same process was repeated 3 times for the hexane-insoluble layer (water layer) to collect the hexane-insoluble fraction and the soluble fraction.
1.2.클로로포름 가용성 분획분리1.2.Chloroform-soluble fraction separation
헥산불용성 분획(수층)에 클로로포름 5L를 가하여 섞은 후에 클로로포름가용성 분획 및 불용성 분획을 분리하였고, 클로로포름 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 클로로포름 불용성 분획 및 가용성 분획을 수집하였다.After adding 5 L of chloroform to the hexane-insoluble fraction (aqueous layer) and mixing, the chloroform-soluble fraction and the insoluble fraction were separated, and the same process was repeated 3 times for the chloroform-insoluble layer (aqueous layer) to collect the chloroform-insoluble fraction and the soluble fraction.
1.3.에틸아세테이트 가용성 분획분리1.3. Ethyl acetate soluble fraction separation
클로로포름 불용성 분획(수층)에 에틸아세테이트 5L를 가하여 섞은 후에 에틸아세테이트 가용성 분획 및 불용성 분획을 분리하였고, 에틸아세테이트 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 에틸아세테이트 불용성 분획 및 가용성 분획를 수집하였다.After adding 5 L of ethyl acetate to the chloroform-insoluble fraction (aqueous layer) and mixing, the ethyl acetate soluble and insoluble fractions were separated. did.
1.4. 부탄올 가용성 분획분리1.4. Butanol-soluble fraction separation
에틸아세테이트 불용선 분획(수층)에 부탄올 5L를 가하여 섞은 후에 부탄올 가용성 분획 및 불용성 분획을 분리하였고, 부탄올 불용성층을 대상으로 동일한 공정을 3번 반복하여 부탄올 불용성 분획 및 가용성 분획을 수집하였다.After adding and mixing 5 L of butanol to the ethyl acetate insoluble fraction (water layer), the butanol soluble fraction and the insoluble fraction were separated, and the same process was repeated 3 times for the butanol insoluble layer to collect the butanol insoluble fraction and the soluble fraction.
1.5 붉가시나무 열수추출물 및 분획물 수득1.5 Obtained hot-water extracts and fractions from Red bramble
붉가시나무 열수추출물에서 헥산 가용성 분획, 클로로포름 가용성 분획, 에틸아세테이트 가용성 분획 및 부탄올 가용성 분획을 감압 농축한 후에 동결건조하여 헥산분획, 클로로포름 분획, 에틸아세테이트 분획, 부탄올 분획을 얻어 시료로 사용하였다.The hexane-soluble fraction, the chloroform-soluble fraction, the ethyl acetate-soluble fraction and the butanol-soluble fraction were concentrated under reduced pressure from the hot-water extract of Red bramble, and then freeze-dried to obtain a hexane fraction, a chloroform fraction, an ethyl acetate fraction, and a butanol fraction, which were used as samples.
실시 예 2. 붉가시나무 추출물의 세포 독성실험결과. Example 2. Results of cytotoxicity test of Red bramble extract.
도 4는 붉가시나무 추출물의 세포 독성실험결과를 나타낸다. 붉가시나무 열수 추출물에 대한 사람피부각질세포(HaCaT)의 세포생존률을 측정하기 위해 MTT assay를 수행하였다. 우선, 세포배양 배지를 제거한 후 MTT를 1 ㎎/㎖로 포함하는 DMEM 배지를 웰 당 1 ㎖씩 처리하고, 37℃ 습윤한 CO₂배양기에서 4시간 더 배양하였다. 이후 배지를 제거한 후, tetrazolium bromide salt를 제거하고, DMSO 200 ㎕를 분주하여 각 웰에 생성된 포르마잔 크리스탈을 용해시키고, 마이크로 플레이트리더(microplate reader)에서 570 ㎚파장으로 흡광도를 측정하여 세포생존율을 확인하였다.붉가시나무 열수 추출물을 다양한 농도(10, 50, 100, 200 그리고 400μg/ml)별로 처리하고 24시간을 처리한 결과, 도 4에 나타낸 바와 같이, 각각 97.86±0.54%, 96.21±1.88%, 95.32±1.37%, 90.50±2.13의 세포증식능을 확인하였다. 이는 시료를 처리하지 않은 정상군과 비교한 결과, 모두 세포독성에 별다른 영향을 나타내지 않는 것으로 확인되었다. Figure 4 shows the cytotoxicity test results of the red bramble extract. MTT assay was performed to measure the cell viability of human skin keratinocytes (HaCaT) to the hot water extract of Red bramble. First, after removing the cell culture medium, DMEM medium containing MTT at 1 mg/ml was treated with 1 ml per well, and cultured for 4 hours in a humidified CO2 incubator at 37°C. After removing the medium, the tetrazolium bromide salt was removed, and 200 μl of DMSO was dispensed to dissolve the formazan crystal generated in each well, and the absorbance was measured at a wavelength of 570 nm in a microplate reader to determine the cell viability. It was confirmed. As a result of treatment with various concentrations (10, 50, 100, 200 and 400 μg/ml) of the hot water extract of Red bramble and 24 hours, as shown in FIG. 4, 97.86±0.54% and 96.21±1.88%, respectively. , 95.32±1.37%, 90.50±2.13 cell proliferative ability was confirmed. As a result of comparison with the normal group not treated with the sample, it was confirmed that none of them had any significant effect on cytotoxicity.
실시 예 3. 붉가시나무 추출물의 콜라겐 합성 증진효과 Example 3. Collagen synthesis enhancing effect of red bramble extract
도 5는 붉가시나무 열수 추출물의 콜라겐 합성 촉진 효과를 나타낸 것이다. 붉가시나무 열수 추출물의 콜라겐 합성 촉진 효과를 평가하기 위해 Procollagen Type I C-Peptide Enzyme Immuno Assay 실험을 수행하였다.Figure 5 shows the collagen synthesis promoting effect of the hot water extract of brambles. A Procollagen Type I C-Peptide Enzyme Immuno Assay experiment was performed to evaluate the collagen synthesis promoting effect of the hot water extract of Red bramble.
사람 피부각질세포인 HaCaT세포를 24-웰 마이크로 플레이트에 각 웰당 5x104 세포가 되도록 접종하고, DMEM 배지에 37℃의 조건에서 70~80% 정도 자랄 때까지 배양하였다. 그 후 혈청이 없는 DMEM 배지로 교체하고 UV-B를 30mJ/cm2처리하여 콜라겐 분해를 유발하였다. 붉가시나무 추출물을 농도별로 10, 50, 100 그리고 200μg/ml처리한 다음 48시간 동안 무혈청 배지에서 추가로 배양하였다. 배양 후, 각 웰의 상층액을 모아 프로콜라겐 (procollagen) 타입 I C-펩타이드 (PICP) 양을 키트 (Takara, 일본국)를 이용하여 측정함으로써 새로 합성된 콜라겐 양을 측정하였다. HaCaT cells, which are human dermal keratinocytes , were inoculated in a 24-well microplate so as to become 5x10 4 cells per well, and cultured in DMEM medium at 37° C. until they grow to 70-80%. Thereafter, it was replaced with serum-free DMEM medium and treated with UV-B 30mJ/cm 2 to induce collagen degradation. 10, 50, 100, and 200 μg/ml of the red bramble extract were treated by concentration and then further cultured in a serum-free medium for 48 hours. After incubation, the amount of newly synthesized collagen was measured by collecting the supernatant from each well and measuring the amount of procollagen type I C-peptide (PICP) using a kit (Takara, Japan).
대조군인 UV-B를 30mJ/cm2를 처리한 경우, PIP 생성량이 정상군의 PIP 생성량에 비해 감소했음을 확인하였다. 반면에 UV-B와 붉가시나무 추출물을 동시에 처리한 실험군의 경우, 붉가시나무 (Quercus acuta Thunb) 추출물의 농도가 증가함에 따라 콜라겐 합성정도가 회복되는 것을 확인할 수 있었고, 이에 본 발명의 붉가시나무 추출물이 탁월한 콜라겐 합성 증진 효과를 나타냄을 알 수 있었다.When the UV-B control group was treated with 30 mJ/cm 2 , it was confirmed that the PIP production amount was decreased compared to the PIP production amount of the normal group. On the other hand, in the case of the experimental group treated with UV-B and bramble extract at the same time, it was confirmed that the degree of collagen synthesis was restored as the concentration of Quercus acuta Thunb extract increased. It was found that it exhibited an excellent collagen synthesis enhancing effect.
실시 예 4. 붉가시나무 추출물의 콜라게네이즈 활성저해 효과Example 4. Collagenase Activity Inhibiting Effect of Red Thorn Extract
도 6은 붉가시나무 열수 추출물의 콜라게네이즈(MMP-1) 활성저해 효과를 나타낸 것이다. 붉가시나무 열수 추출물의 콜라겐 분해 억제능을 평가하기 위해 MMP-1 활성저해 실험을 수행하였다. Figure 6 shows the collagenase (MMP-1) activity inhibitory effect of the hot water extract of Rhododendron. An MMP-1 activity inhibition experiment was performed to evaluate the collagen degradation inhibitory ability of the hot water extract of Red bramble.
사람 피부각질세포주인 HaCaT세포를 96-웰 마이크로 플레이트에 각 웰당 1X 104세포가 되도록 접종하고, DMEM 배지에 37℃의 조건에서 70~80% 정도 자랄 때까지 배양하였다. 그 후 혈청이 없는 DMEM 배지로 교체하고 UV-B를 30mJ/cm2처리하여 콜라게네이즈 활성증가를 유발하였다. 실험군으로 붉가시나무 10, 50, 100, 200㎍/㎖로 처리한 다음 48시간 동안 무혈청 배지에서 추가로 배양하였다. 배양 후, 각 웰의 상층액을 모아 MMP-1 assay kit(R&D systems, 미국)를 사용하여 450nm에서 total MMP-1 효소의 활성을 확인하였다.HaCaT cells, which are human dermal keratinocytes , were inoculated in a 96-well microplate so that 1X 10 4 cells per well were inoculated, and cultured in DMEM medium at 37° C. until 70-80% growth. Thereafter, it was replaced with serum-free DMEM medium and treated with UV-B 30mJ/cm 2 to induce an increase in collagenase activity. The experimental group was treated with 10, 50, 100, and 200 μg/ml of red bramble, and then further cultured in a serum-free medium for 48 hours. After incubation, the supernatant of each well was collected and the activity of total MMP-1 enzyme was confirmed at 450 nm using an MMP-1 assay kit (R&D systems, USA).
대조군인 UV-B를 30mJ/cm2를 처리한 경우, 콜라게네이즈 활성이 정상군의 활성에 비해 증가했음을 확인하였다. 반면에 UVB와 붉가시나무 추출물을 동시에 처리한 실험군의 경우, 붉가시나무 추출물의 농도가 증가함에 따라 콜라게네이즈의 활성이 감소되는 것을 확인할 수 있었다. 이에 본 발명의 붉가시나무 추출물이 UV-B에 의해 증가된 콜라게네이즈 활성 억제에 관여하고 콜라겐 분해 촉진 억제에 관여함으로서 자외선에 의한 피부노화방지 및 개선에 효과가 있음을 확인하였다. 또한 탁월한 콜라겐 합성 증진 효과를 나타냄을 알 수 있었다.When the UV-B control group was treated with 30mJ/cm 2 , it was confirmed that the collagenase activity was increased compared to the activity of the normal group. On the other hand, in the case of the experimental group treated with UVB and bramble extract at the same time, it was confirmed that the activity of collagenase decreased as the concentration of bramble extract increased. Accordingly, it was confirmed that the red bramble extract of the present invention was effective in preventing and improving skin aging caused by UV rays by participating in the inhibition of collagenase activity increased by UV-B and inhibiting the promotion of collagen degradation. In addition, it was found that it exhibits an excellent collagen synthesis enhancing effect.
실시 예 5. 붉가시나무 추출물을 이용한 자외선에 의한 피부노화방지 및 개선용 화장품 제조Example 5. Preparation of cosmetics for preventing and improving skin aging due to ultraviolet rays using a red bramble extract
도 7은 본 발명의 붉가시나무 원물 및 추출물을 이용하여 제조한 자외선에 의한 피부노화방지 및 개선용 화장품 로션 제형을 나타낸다.7 shows a cosmetic lotion formulation for preventing and improving skin aging by ultraviolet rays prepared by using the red bramble extract and extract of the present invention.
1) 붉가시나무 추출물을 포함하는 보습제 미스트의 제조1) Preparation of moisturizing mist containing red bramble extract
정제수 100 ml에 대해 상기 붉가시나무 열수 추출물 10 mg 및 pH 조절제로서 트리에탄올아민을 1 mg을 혼합하여 미스트를 제조하였다. 이 미스트의 pH는 7.5이고, 상기 미스트를 성인의 피부에 도포하여 하용하였다. To 100 ml of purified water, 10 mg of the hot water extract of Red bramble and 1 mg of triethanolamine as a pH adjuster were mixed to prepare a mist. The pH of this mist was 7.5, and the mist was applied to the skin of an adult and then used.
2) 붉가시나무 추출물을 포함하는 로션의 제조2) Preparation of lotion containing red bramble extract
정제수 100 ml에 대해 실시예 1에서 제조된 붉가시나무 열수 추출물 10 mg, pH 조절제로서 트리에탄올아민 1 mg, 알로에 베라 추출물 0.5 mg, 살리실산 0.5 mg, 알란토인 0.5 mg, 호호바 오일 0.5 mg, 토코페롤 0.5 mg을 넣고, 50℃로 가열한 후, 식물성오일로서 시어버터(Shea butter) 20 ml 및 유화제로서 세틸알콜 20ml을 혼합한 유상층과 혼합하여 유화시킴으로써 로션을 제조하였다.To 100 ml of purified water, 10 mg of the hot water extract prepared in Example 1, 1 mg of triethanolamine, 0.5 mg of aloe vera extract, 0.5 mg of salicylic acid, 0.5 mg of allantoin, 0.5 mg of jojoba oil, and 0.5 mg of tocopherol as a pH adjuster were added. After heating to 50° C., a lotion was prepared by emulsifying it by mixing it with an oily layer in which 20 ml of shea butter as a vegetable oil and 20 ml of cetyl alcohol as an emulsifier were mixed.
이외에도 본원발명의 붉가시나무 추출물을 이용하여 다음과 같은 자외선에 의한 피부노화방지 및 개선용 화장품 제조할 수 있다. 화장품의 피부외용제 제형은 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스쳐로션, 영양로션, 맛사지크림, 영양크림, 모이스처크림, 핸드크림, 선크림, 파운데이션, 에센스, 영양에센스, 팩, 비누, 샴푸, 린스, 헤어트리트먼트, 화장용물티슈, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션 및 바디클렌저로 구성된 군으로부터 선택되는 어느 하나의 제형으로 이루어지는 것일 수 있다.In addition, by using the red bramble extract of the present invention, it is possible to prepare cosmetics for preventing and improving skin aging due to the following ultraviolet rays. Cosmetics for external application for skin are skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, sun cream, foundation, essence, nourishing essence, pack. , soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.
본 발명은 붉가시나무 (Quercus acuta Thunb) 추출물을 유효성분으로 포함하는 자외선에 의한 피부노화방지 및 개선용 화장료 조성물을 제공함으로서 콜라겐합성을 증가시키고, 콜라게나아제의 활성 저해 효과가 우수하며 독성 및 부작용 없이 안전하게 사용될 수 있는 피부노화방지 및 개선용 화장료 제품 생산 및 관련 산업의 이윤을 창출할 수 있어 산업상 이용가능성이 있다.The present invention increases collagen synthesis by providing a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays comprising an extract of Quercus acuta Thunb as an active ingredient, and has excellent effect of inhibiting collagenase activity, toxicity and side effects It has industrial applicability because it can produce cosmetic products for skin aging prevention and improvement that can be safely used without the need for production and profit in related industries.
Claims (3)
- 붉가시나무 (Quercus acuta Thunb) 열매의 열수추출물을 유효성분으로 포함하는 것을 특징으로 하는 자외선에 의한 피부손상 예방 또는 개선용 화장료 조성물A cosmetic composition for preventing or improving skin damage caused by ultraviolet rays, characterized in that it contains a hot water extract of Quercus acuta Thunb fruit as an active ingredient
- 제1항에 있어서, 상기 조성물은 피부외용제 제형인 것을 특징으로 하는 자외선에 의한 피부손상 예방 또는 개선용 화장료 조성물The cosmetic composition for preventing or improving skin damage caused by ultraviolet rays according to claim 1, wherein the composition is an external preparation for skin.
- 제2항에 있어서, 피부외용제 제형은 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 밀크로션, 모이스쳐로션, 영양로션, 맛사지크림, 영양크림, 모이스처크림, 핸드크림, 선크림, 파운데이션, 에센스, 영양에센스, 팩, 비누, 샴푸, 린스, 헤어트리트먼트, 화장용물티슈, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션 및 바디클렌저로 구성된 군으로부터 선택되는 어느 하나의 제형으로 이루어지는 것을 특징으로 하는 자외선에 의한 피부손상 예방 또는 개선용 화장료 조성물According to claim 2, wherein the skin external formulation is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, sun cream, foundation, essence, Ultraviolet light, characterized in that it consists of any one formulation selected from the group consisting of nutritional essence, pack, soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Cosmetic composition for preventing or improving skin damage caused by
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| JP2004359664A (en) * | 2003-05-09 | 2004-12-24 | Kotobuki Chemical:Kk | Composition for skin care preparation for external use and skin care preparation for external use |
| KR20090118154A (en) * | 2008-05-13 | 2009-11-18 | 재단법인 제주하이테크산업진흥원 | Composition for anti-oxidation and anti-cancer comprising the extract of quercus acuta as active ingredient |
| KR20110055940A (en) * | 2009-11-20 | 2011-05-26 | 재단법인 제주테크노파크 | Composition for improving atopy dermatitis using a extract of humulus japonicus or a extract of quercus acuta |
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| JP2004359664A (en) * | 2003-05-09 | 2004-12-24 | Kotobuki Chemical:Kk | Composition for skin care preparation for external use and skin care preparation for external use |
| KR20090118154A (en) * | 2008-05-13 | 2009-11-18 | 재단법인 제주하이테크산업진흥원 | Composition for anti-oxidation and anti-cancer comprising the extract of quercus acuta as active ingredient |
| KR20110055940A (en) * | 2009-11-20 | 2011-05-26 | 재단법인 제주테크노파크 | Composition for improving atopy dermatitis using a extract of humulus japonicus or a extract of quercus acuta |
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