KR102147623B1 - Cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing Quercus acuta Thunb extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing and alleviating skin aging caused by ultraviolet rays, the cosmetic composition comprising containing a Quercus acuta fruit extract as an active ingredient. It has been confirmed that in the cosmetic composition for preventing and alleviating skin aging caused by ultraviolet rays according to the present invention, when HaCaT cells, which are included in a human skin keratin cell line, is treated with a UVB, which is a control group, a collagenase activity is enhanced compared to that of a normal group. However, in the case of an experimental group simultaneously treated with a UVB and a Quercus acuta extract, it has been confirmed that the activity of collagenase decreases as the concentration of a Quercus acuta extract increases. Accordingly, the present invention can be used as a cosmetic composition for preventing and alleviating skin aging caused by UV rays having an excellent inhibitory effect on collagenase and capable of being safely used without toxicity and side effects.

Description

Cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing Quercus acuta Thunb extract}

The present invention relates to a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays, comprising a hot water extract of Quercus acuta Thunb fruit as an active ingredient. More specifically, the cosmetic composition for preventing and improving skin aging caused by ultraviolet rays according to the present invention is excellent in inhibiting the activity of collagenase when UV-B is treated on HaCaT cells, which is a human skin keratin cell line, and increases collagen synthesis. It relates to a cosmetic composition for preventing and improving skin aging that can be safely used without toxicity and side effects.

In sunlight, there are visible, infrared, and ultraviolet rays.Ultraviolet is divided into three types: ultraviolet A (320-400nm), ultraviolet B (280-320nm), and ultraviolet C (190-280nm) depending on the wavelength. . In general, ultraviolet C is absorbed, scattered and filtered in the ozone layer in the upper part of the atmosphere, so it does not affect the natural photochemical reaction.

The ultraviolet rays that mainly affect the human body are ultraviolet A (Ultraviolet-A) and ultraviolet B (Ultraviolet-B). Ultraviolet A is primarily emitted from the sun, but it is also emitted by artificial lamps and penetrates deeply into the epidermis and dermis of the skin. Therefore, ultraviolet A is currently being watched as the factor that most affects the skin. Also, ultraviolet B irradiation is more dangerous than ultraviolet A. In other words, it is known that UV B has a shorter wavelength than UV A and cannot penetrate deep into the skin, but has much stronger energy than UV A, so it is known as a wavelength that causes sharp erythema on the skin surface and promotes photoaging of the skin.

Ultraviolet B damages DNA and connective tissue, causing photoaging. The period of strong UV rays is from April to September, and the peak is from May to June. Skin aging caused by continuous exposure to such ultraviolet rays is called photoaging, and the most prominent symptoms of photoaging are fine wrinkles, spots, and pigmentation, which are proportional to the time and intensity of exposure to the sun.

One of the causes of skin damage caused by UV rays is Matrix Metal, an enzyme that breaks down collagen or gelatin, which are components of the skin's extracellular matrix (ECM), by sending a signal to the cell signaling system by reactive oxygen species formed by UV rays. By producing proteinase (matrix metalloproteinase, MMP), it destroys collagen or gelatin, causing wrinkles to form on the skin. The skin of a farmer or a fisherman is a symbolic indication of the effects of chronic UV rays. The characteristic features of the skin are black, stiff to the touch, and deep wrinkles. When this condition becomes more severe, it causes skin cancer. This change in skin is called photoaging, distinguishing it from natural aging.

Meanwhile, the domestic natural resource, Quercus acuta THUNB. ) Is an evergreen broad-leaved arboreous tree of the genus of the oak family oak that grows a lot in the sunny foothills and valleys of 170-500m above sea level in Jeollanam-do, North Gyeongsang and Jeju-do. It is a tree that grows over 20m in height and 60cm in diameter, and brown hairs are densely spread on young branches. Leaves are alternate, long oval or long ovate, 7 to 13 cm long, and flat edges. The flower is a single family flower that blooms in May, and the fruit is 2cm long and ripens in October of the following year. Because the color of the wood has a reddish color, it was named red thorn.

In the present invention, when UV-B is applied to HaCaT cells, a skin keratin cell line, with a composition using red thorn, a natural plant raw material in Korea, it has excellent inhibitory effect on collagenase activity and increases collagen synthesis so that it is safe without toxicity and side effects. It is intended to provide a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays that can be used.

Korean Patent Publication No. 10-1068763 relates to a composition having an effect of inhibiting skin aging by ultraviolet rays containing elagic acid. More specifically, by containing ellagic acid, collagen degradation of skin fibroblasts by ultraviolet B wavelength and collagenase activity, which is matrix metalloproteinase (MMP), are inhibited, and hairless mice (hairless A composition that supports the collagen fibers of (mouse) and can improve skin wrinkles and inflammation caused by UV rays by reducing the expression of adhesion molecules in keratinocytes and secretion of inflammatory factors in the skin tissues. Is done. Korean Patent Publication No. 10-1426592 relates to a composition for inhibiting UV-induced skin aging containing a lotus meat extract as an active ingredient, comprising: pulverizing Nelumbinis semen; adding water to the pulverized lotus meat to have a lotus meat content Mixing to be 0.5 to 4% by weight; And heating the mixture for 10 to 40 minutes to extract hot water. Korean Patent Publication No. 10-1223749 discloses a composition for improving atopic dermatitis using an extract of Yulcho or Redthorn. In Korean Patent Publication No. 10-0997674, the stem with red thorn leaves is shaded, then crushed with a grinder to make powder, and this powder is extracted with ethanol three times for 1 hour using ultrasonic waves, and then the supernatant of the extract Was recovered, concentrated under reduced pressure, and the concentrate was suspended in distilled water, and the suspension was sequentially extracted with solvents such as hexane, dichloromethane, ethyl acetate, butanol, and water. A dragon composition is disclosed.

To provide a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays containing hot water extract of red thorn fruit that can be safely used without toxicity and side effects using red thorn fruit, a natural raw material, for the purpose of utilizing Korea's natural plant resources. do.

In order to achieve the object of the present invention, the present invention provides a cosmetic composition for preventing and improving skin aging due to ultraviolet rays, characterized in that it contains a hot water extract of Quercus acuta Thunb fruit.

In order to solve the above problem, the red thorn of the present invention extracts at least one selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, ethyl acetate, acetone, ether, chloroform, benzene, hexane, dichloromethane, and mixtures thereof. It provides a cosmetic composition for preventing and improving skin aging due to ultraviolet rays containing a red thorn extract extracted with a solvent.

In addition, prevention of skin aging by ultraviolet rays containing the red thorn extract fractionated by using any one of hexane, chloroform, dichloromethane and ethyl acetate as a non-polar solvent as a fractionation solvent in the crude red thorn extract extracted using the extraction solvent. And it provides a cosmetic composition for improvement.

It provides a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays, which is an external formulation for skin containing 0.01 to 50.0% by weight of a cosmetic composition for preventing and improving skin aging by ultraviolet rays containing the red thorn extract of the present invention. The cosmetic composition for preventing and improving skin aging caused by UV rays of the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, sun cream , Foundation, essence, nutritional essence, pack, soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser I can.

The composition containing the red thorn extract of the present invention confirmed that when the human skin keratin cell line HaCaT cells were treated with UV-B as a control, collagenase activity was increased compared to that of the normal group, but UV-B and red thorns In the case of the experimental group treated with the tree extract at the same time, it was confirmed that the activity of collagenase decreased as the concentration of the red thorn extract increased, and the effect of inhibiting the activity of collagenase was excellent, and by increasing collagen synthesis, there was no toxicity and side effects. It was confirmed that it can be safely used as a cosmetic composition for preventing and improving skin aging due to ultraviolet rays.

1 is a photograph of a fruit that is a raw material of the red thorn used in the present invention.
2 is a schematic diagram showing a process of preparing extracts and fractions of Quercus acuta Thunb using a raw material of red thorns as a solvent.
3 is a photograph of the powder of red thorn extract.
Figure 4 shows the results of the cytotoxicity test of hot water extract of red thorns.
Figure 5 shows the effect of promoting collagen synthesis of hot water extract of red thorns.
Figure 6 shows the effect of inhibiting collagenase activity of hot water extract of red thorns.
7 shows a cosmetic lotion formulation for preventing and improving skin aging due to ultraviolet rays prepared using the original red thorn extract and extract of the present invention.

The detailed configuration related to the cosmetic composition for preventing and improving skin aging by ultraviolet rays containing the red thorn fruit extract of the present invention and the accompanying drawings will be described in detail as follows.

FIG. 1 is a photograph of a fruit, which is a raw material of a red thorn, used in the present invention, and FIG. 2 is a schematic diagram showing a process of preparing extracts and fractions of Quercus acuta Thunb using a raw material of red thorn. As the raw material of the red thorn used in the present invention, a dried raw material supplied through a commercially available pharmaceutical company was used.

Example 1. Preparation of extracts and fractions of Quercus acuta Thunb

The extract of Quercus acuta Thunb is prepared by soluble in water or any one selected from alcohols having 1 to 5 carbon atoms and a mixed solvent thereof. 7.5L distilled water was added to 500 g of dried red thorns and heated at 100° C. for 3 hours to extract hot water. The extracted hot water extract was concentrated under reduced pressure and freeze-dried to obtain 91.2 g of a hot water freeze-dried product of Quercus acuta Thunb. 3 shows a picture of the powder of red thorn extract.

Preparation of polar solvent and non-polar solvent-soluble fractions of red thorn After completely dissolving the hot water extract of red thorn in 1 L of distilled water, it was put into a fractionation funnel and 1 L of hexane was added to separate the water layer and the hexane layer, and the same process was repeated three times. Through the same process, chloroform, ethyl acetate, and butanol were sequentially added to obtain each fraction, and each obtained fraction was filtered through a vacuum filtration device, concentrated, and freeze-dried to completely remove the solvent. It was used later in this experiment.

1.1. Hexane-soluble fraction separation

After completely dissolving the red thorn hot-water extract in 1 L of distilled water, it was placed in a fractionation funnel and 1 L of hexane was added to separate the hexane-insoluble layer (aqueous layer) and the hexane-soluble layer. Again, the same process was repeated three times for the hexane insoluble layer (aqueous layer) to collect the hexane insoluble fraction and the soluble fraction.

1.2. Chloroform soluble fraction separation

After 5L of chloroform was added to the hexane-insoluble fraction (aqueous layer) and mixed, the chloroform-soluble fraction and the insoluble fraction were separated, and the same process was repeated three times for the chloroform insoluble layer (aqueous layer) to collect the chloroform insoluble fraction and the soluble fraction.

1.3. Ethyl acetate soluble fraction separation

After 5L of ethyl acetate was added to the chloroform insoluble fraction (aqueous layer) and mixed, the ethyl acetate soluble fraction and the insoluble fraction were separated, and the same process was repeated 3 times for the ethyl acetate insoluble layer (aqueous layer) to collect the ethyl acetate insoluble fraction and the soluble fraction I did.

1.4. Butanol-soluble fraction separation

After adding and mixing 5L of butanol to the ethyl acetate insoluble fraction (aqueous layer), the butanol-soluble fraction and the insoluble fraction were separated, and the butanol-insoluble fraction and the soluble fraction were collected by repeating the same process three times for the butanol-insoluble layer.

1.5 Obtaining Hot Water Extract and Fraction from Redthorn

Hexane-soluble fraction, chloroform-soluble fraction, ethyl acetate-soluble fraction, and butanol-soluble fraction were concentrated under reduced pressure from the hot-water extract of red thorns, and then freeze-dried to obtain a hexane fraction, a chloroform fraction, an ethyl acetate fraction, and a butanol fraction and used as samples.

Example 2. Cytotoxicity test results of red thorn extract.

Figure 4 shows the results of the cytotoxicity test of the red thorn extract. MTT assay was performed to measure the cell viability of human skin keratinocytes (HaCaT) for the hot water extract of Redthorn. First, after removing the cell culture medium, a DMEM medium containing 1 mg/ml of MTT was treated with 1 ml per well, and cultured for 4 hours in a CO2 incubator humidified at 37°C. After removing the medium, tetrazolium bromide salt was removed, and 200 µl of DMSO was dispensed to dissolve the formazan crystal generated in each well, and the cell viability was measured by measuring the absorbance at a wavelength of 570 nm in a microplate reader. As a result of treatment with various concentrations (10, 50, 100, 200, and 400 μg/ml) of the hot water extract of Red thorn and 24 hours, as shown in Fig. 4, respectively, 97.86±0.54% and 96.21±1.88% , 95.32±1.37%, 90.50±2.13 cell proliferation ability was confirmed. As a result of comparison with the normal group without treatment of the sample, it was found that none of the samples had a significant effect on cytotoxicity.

Example 3. The effect of enhancing collagen synthesis of red thorn extract

Figure 5 shows the effect of promoting collagen synthesis of hot water extract of red thorns. A Procollagen Type I C-Peptide Enzyme Immuno Assay experiment was performed to evaluate the collagen synthesis promoting effect of the hot water extract of Redthorn.

HaCaT cells, which are human skin keratinocytes, were inoculated into a 24-well microplate so as to be 5×10 ⁴ cells per well, and cultured in DMEM medium at 37° C. until about 70-80% growth. Then, it was replaced with serum-free DMEM medium, and UV-B was treated with 30mJ/cm ² to induce collagen degradation. The red thorn extract was treated with 10, 50, 100 and 200 μg/ml for each concentration, and then further cultured in a serum-free medium for 48 hours. After incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using a kit (Takara, Japan) to measure the amount of newly synthesized collagen.

When the control group UV-B was treated with 30mJ/cm ² , it was confirmed that the amount of PIP produced was decreased compared to that of the normal group. On the other hand, in the case of the experimental group treated with UV-B and red thorn extract at the same time, it was confirmed that the degree of collagen synthesis was recovered as the concentration of the extract of Quercus acuta Thunb was increased, and thus the red thorn extract of the present invention It was found that it exhibited excellent collagen synthesis enhancing effect.

Example 4. Effect of red thorn extract on inhibiting collagenase activity

6 shows the effect of inhibiting collagenase (MMP-1) activity of hot water extract of red thorns. In order to evaluate the ability to inhibit collagen degradation of the hot water extract of red thorns, an MMP-1 activity inhibition experiment was performed.

HaCaT cells, a human skin keratin cell line, were inoculated in a 96-well microplate to 1×10 ⁴ cells per well, and cultured in DMEM medium at 37° C. until about 70-80% growth. Then, it was replaced with serum-free DMEM medium, and UV-B was treated with 30mJ/cm ² to induce an increase in collagenase activity. As an experimental group, red thorns were treated with 10, 50, 100, and 200 µg/ml, and then further cultured in a serum-free medium for 48 hours. After incubation, the supernatant of each well was collected and the activity of the total MMP-1 enzyme was confirmed at 450 nm using an MMP-1 assay kit (R&D systems, USA).

When the control group UV-B was treated with 30mJ/cm ² , it was confirmed that the collagenase activity was increased compared to that of the normal group. On the other hand, in the case of the experimental group treated with UVB and red thorn extract at the same time, it was confirmed that the activity of collagenase decreased as the concentration of red thorn extract increased. Accordingly, it was confirmed that the red thorn extract of the present invention is effective in preventing and improving skin aging due to ultraviolet rays by being involved in inhibiting the collagenase activity increased by UV-B and inhibiting the promotion of collagen degradation. In addition, it was found that it exhibited excellent collagen synthesis enhancing effect.

Example 5. Manufacture of cosmetics for preventing and improving skin aging by ultraviolet rays using red thorn extract

7 shows a cosmetic lotion formulation for preventing and improving skin aging due to ultraviolet rays prepared using the original red thorn extract and extract of the present invention.

1) Preparation of moisturizing mist containing red thorn extract

Mist was prepared by mixing 10 mg of the hot water extract of Redthorn and 1 mg of triethanolamine as a pH adjuster with respect to 100 ml of purified water. The pH of this mist was 7.5, and the mist was applied and used on the skin of an adult.

2) Preparation of lotion containing red thorn extract

With respect to 100 ml of purified water, 10 mg of the hot water extract of Redthorn prepared in Example 1, 1 mg of triethanolamine, 0.5 mg of aloe vera extract, 0.5 mg of salicylic acid, 0.5 mg of allantoin, 0.5 mg of jojoba oil, and 0.5 mg of tocopherol were added as a pH adjuster. , After heating to 50°C, a lotion was prepared by emulsifying by mixing with an oily layer in which 20 ml of shea butter as vegetable oil and 20 ml of cetyl alcohol as an emulsifier were mixed.

In addition, it is possible to manufacture cosmetics for preventing and improving skin aging caused by ultraviolet rays as follows by using the red thorn extract of the present invention. The cosmetic formulation for external skin is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, sun cream, foundation, essence, nutrition essence, pack , Soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.It may be made of any one formulation selected from the group consisting of.

The present invention provides a cosmetic composition for preventing and improving skin aging caused by ultraviolet rays comprising an extract of Quercus acuta Thunb as an active ingredient, thereby increasing collagen synthesis, has excellent inhibitory effect on collagenase activity, and has toxicity and side effects. It has industrial applicability because it can produce profits in related industries and produce cosmetic products for preventing and improving skin aging that can be safely used without.

Claims (5)

A cosmetic composition for preventing or improving skin damage caused by ultraviolet rays, characterized in that it contains hot water extract of the fruit of Quercus acuta Thunb as an active ingredient delete delete The cosmetic composition for preventing or improving skin damage caused by ultraviolet rays according to claim 1, wherein the composition is an external preparation for skin. The formulation of claim 4, wherein the external preparation for skin is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, sun cream, foundation, essence, nutrition. Essence, pack, soap, shampoo, conditioner, hair treatment, cosmetic wipes, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser. Cosmetic composition for preventing or improving skin damage caused by

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