WO2021128996A1 - 一种含艾叶提取物的天然植物抑菌软膏及其制备方法 - Google Patents
一种含艾叶提取物的天然植物抑菌软膏及其制备方法 Download PDFInfo
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- WO2021128996A1 WO2021128996A1 PCT/CN2020/116718 CN2020116718W WO2021128996A1 WO 2021128996 A1 WO2021128996 A1 WO 2021128996A1 CN 2020116718 W CN2020116718 W CN 2020116718W WO 2021128996 A1 WO2021128996 A1 WO 2021128996A1
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- Prior art keywords
- wormwood
- extract
- natural plant
- oil
- mugwort leaf
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Definitions
- the invention belongs to the field of antibacterial ointment products, and specifically relates to a natural plant antibacterial ointment containing mugwort leaf extract and a preparation method thereof.
- mosquitoes Every spring and summer, as the climate warms up, mosquitoes breed in large numbers, and people's chances of being bitten by mosquitoes increase significantly. Mosquito bites have become a major problem that bothers us, often leading to itching and redness. At the same time, mosquitoes often carry pathogenic bacteria such as Escherichia coli and Staphylococcus aureus. The bite of mosquitoes can easily lead to bacterial infection, which seriously affects our work and life. At present, the most common treatments for inflammation and bacterial infections caused by mosquito bites on the market are generally chemical treatments such as glucocorticoids and antihistamines, which have relatively large side effects and low safety.
- the present invention provides a natural plant containing mugwort extract.
- Plant antibacterial ointment The components of the antibacterial ointment are of natural origin, do not contain chemically synthesized antibacterial agents and preservatives, are safe, non-toxic, non-irritating, and have obvious effects of antibacterial and antipruritic effects.
- the present invention also provides a method for preparing the above-mentioned natural plant antibacterial ointment containing mugwort leaf extract.
- a natural plant antibacterial ointment containing mugwort leaf extract includes the following components:
- the wormwood extract includes wormwood hydrosol, wormwood wax and wormwood oil.
- the natural plant antibacterial ointment contains 15-30% wormwood pure dew, 0.5%-1% wormwood wax, and 1%-2% wormwood heavy oil.
- the wormwood extract includes wormwood pure dew, wormwood leaf wax and wormwood heavy oil; and by weight percentage, the natural plant antibacterial ointment contains: wormwood pure dew 20%, wormwood wax 0.5%, and wormwood heavy oil 2% .
- the wormwood pure dew is obtained by the following method:
- the wormwood wax and the wormwood heavy oil are prepared by the following methods:
- the thickener includes gum arabic or xanthan gum.
- the emulsifier includes natural source cetearyl alcohol, coco glucoside, arachidyl alcohol, behenyl alcohol or arachidyl glucoside.
- the method for preparing a natural plant antibacterial ointment containing mugwort leaf extract as described in any one of the above includes the following steps:
- step (3) Add the water phase of step (1) to the oil phase of step (2), stir while adding, and after mixing uniformly, homogenize and cool to obtain an ointment base;
- the heating is to 80°C; the heat preservation time is 30-35 min.
- the rotation speed of the homogenization is 1500-3000 r/min, the time is 30 min, and the temperature is maintained above 60° C. during the homogenization process.
- the cooling is natural cooling to 40°C.
- the rotation speed of the stirring is 800-1200 r/min, and the time is 10-20 min.
- the present invention has the following advantages and beneficial effects:
- the natural plant antibacterial ointment containing mugwort leaf extract of the present invention mainly contains mugwort leaf extract, portulaca extract, sophora flavescens root powder, dipotassium glycyrrhizinate, camellia seed oil, menthol, and lavender essential oil
- the natural plant antibacterial ointment containing mugwort leaf extract of the present invention has obvious antibacterial, anti-inflammatory and anti-inflammatory properties. Pain and antipruritic effect. It has obvious inhibitory effect on Escherichia coli and Staphylococcus aureus, and has skin care effects. It does not contain chemical synthetic antibacterial agents and preservatives. It is green, safe, non-toxic and non-irritating.
- the natural plant antibacterial ointment containing mugwort leaf extract of the present invention includes the following components by weight percentage:
- the wormwood extract includes wormwood hydrosol, wormwood wax and wormwood oil.
- the natural plant antibacterial ointment contains 15-30% wormwood hydrosol, 0.5%-1% wormwood wax, and 1%-2% wormwood heavy oil by weight percentage.
- Mugwort leaves are dried leaves of Artemisia wormwood in the genus Compositae. Its extract has bactericidal and antibacterial effects; Portulaca oleracea extract has anti-irritant, anti-allergic and anti-inflammatory active ingredients; Sophora flavescens contains Both matrine and oxymatrine have been shown to have antibacterial and anti-inflammatory activities; dipotassium glycyrrhizinate has anti-inflammatory, anti-allergic, and moisturizing effects; camellia seed oil has a good health effect on the skin, and also has sterilization, detoxification and acceleration Wound healing and other effects; Menthol is a commonly used anti-inflammatory and analgesic ingredient, often used in mosquito bites preparations; Lavender essential oil and Paeonia suffruticosa extract have antibacterial effects, and pentanediol has a synergistic effect.
- the natural plant antibacterial ointment containing mugwort leaf extract of the present invention mainly contains mugwort leaf extract, portulaca extract, sophora flavescens root powder, dipotassium glycyrrhizinate, camellia seed oil, menthol, lavender essential oil and peony Skin extracts and other highly effective antibacterial and anti-inflammatory components, and these components are all natural sources, non-toxic and non-irritating; thus, the natural plant antibacterial ointment containing mugwort leaf extract of the present invention has obvious antibacterial properties , Anti-inflammatory, analgesic, and anti-itching effects. It has obvious inhibitory effects on E. coli and Staphylococcus aureus. It can be used for disinfection and anti-itching of mosquito bites. It also has skin care effects and does not contain chemical synthesis and antibacterial. Agents and preservatives, green, safe, non-toxic and non-irritating.
- a natural plant antibacterial ointment containing mugwort leaf extract contains the following components:
- Wormwood Hydrosol 20% Mugwort Leaf Wax 0.5%, Mugwort Leaf Heavy Oil 2%, Purslane Extract 2%, Sophora flavescens Root Powder 0.05%, Dipotassium Glycyrrhizinate 0.2%, Camellia Seed Oil 8%, Menthol 1% , Pentanediol 3%, lavender essential oil 0.2%, tree peony bark extract 1%, cetearyl alcohol 5%, gum arabic 1.2%, the balance is water.
- the wormwood pure dew is obtained by the following method:
- the wormwood wax and the wormwood oil are obtained by the following methods:
- a natural plant antibacterial ointment containing mugwort leaf extract contains the following components:
- Mugwort Hydrosol 15% Mugwort Wax 1%, Mugwort Leaf Heavy Oil 1%, Portulaca Extract 2%, Sophora flavescens Root Powder 0.05%, Dipotassium Glycyrrhizinate 0.2%, Camellia Seed Oil 10%, Menthol 1% , Pentylene glycol 3%, Lavender essential oil 0.2%, Paeonia suffruticosa extract 0.5%, Coco glucoside 2%, Xanthan gum 1.2%, the balance is water.
- the wormwood pure dew is obtained by the following method:
- the wormwood wax and the wormwood oil are obtained by the following methods:
- a natural plant antibacterial ointment containing mugwort leaf extract contains the following components:
- Mugwort Hydrosol 30% Mugwort Leaf Wax 0.5%, Mugwort Leaf Heavy Oil 2%, Portulaca Extract 3%, Sophora flavescens Root Powder 0.05%, Dipotassium Glycyrrhizinate 0.2%, Camellia Seed Oil 8%, Menthol 2% , Pentanediol 3%, lavender essential oil 0.2%, tree peony bark extract 0.8%, cetearyl alcohol 2%, xanthan gum 1.2%, the balance is water.
- the wormwood pure dew is obtained by the following method:
- the wormwood wax and the wormwood oil are obtained by the following methods:
- the antibacterial ointment prepared in Examples 1-3 was tested for antibacterial performance according to the method in Appendix C4 of "GB 15979-2002 Hygienic Standard for Disposable Sanitary Products". The test results are shown in Table 1.
- test results show that the bacteriostatic ointment prepared in Examples 1-3 has a bacteriostatic rate of greater than 90% against Escherichia coli, and has a strong bacteriostatic effect; the bacteriostatic rate against Staphylococcus aureus, the bacteriostatic rate of Example 1
- the bacteriostatic rate of the ointment is greater than 90%
- the bacteriostatic rates of the bacteriostatic ointment of Example 2 and Example 3 are both greater than 50% and less than 90%, indicating that it has bacteriostatic effect.
- Example 1 of the present invention The antibacterial ointment prepared in Example 1 of the present invention was tested according to the "Disinfection Technical Specification (2002 Edition)" skin allergy inspection method, and no skin allergy was seen.
- the specific testing content is as follows:
- Test method Select 48 guinea pigs and randomly divide them into 3 groups (negative control group, positive control group and experimental group) according to their body weight, and perform 3cm*3cm hair removal treatment on the back sample application site 24h before the test. On D0, D7, and D14, apply the inducer to the 2cm*2cm area on the left side of the back at a dose of 0.5ml/animal, cover it with a layer of weighing paper, and fix it with non-irritating tape for 6h, then remove and Wash; Finally, on D28 days, the animals were challenged with the corresponding triggering agent.
- test results in Table 3 show that, compared with the positive control group, the animals in the negative control group and the experimental group have no skin allergies such as erythema or edema after induction and challenge, indicating that the antibacterial ointment prepared by the present invention is safe and non-irritating.
- the antibacterial ointment prepared in Example 1 was applied and wiped on the skin after bites by ants and mosquitoes in time, which can instantly calm itching and relieve pain, and eliminate redness, swelling, papules and other symptoms. Tested by 4 people, the efficiency reached 100%. It shows that the antibacterial ointment prepared by the present invention has potential anti-inflammatory and antipruritic effects.
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Abstract
一种含有艾叶提取物的天然抑菌软膏及其制备方法。该软膏主要包含艾叶提取物、马齿苋提取物、苦参根粉、甘草酸二钾、山茶籽油、薄荷脑、薰衣草精油以及牡丹皮提取物等多种高效的抑菌消炎组分。这些组分均为天然来源，无毒、无刺激。该软膏具有明显的抑菌、消炎、镇痛、止痒的效果，对大肠杆菌及金黄色葡萄球菌均具有明显的抑制效果，且具有护肤作用，并且不含化学合成抑菌剂和防腐剂，绿色、安全、无毒、无刺激。
Description
本发明属于抑菌软膏用品领域，具体涉及一种含艾叶提取物的天然植物抑菌软膏及其制备方法。
每到春夏季节，随着气候的回暖，蚊虫大量滋生，人们受到蚊虫叮咬的几率显著增加，蚊虫叮咬也就成为了困扰我们的一大难题，经常导致瘙痒与红肿的发生。同时蚊虫常携带有大肠杆菌以及金黄色葡萄球菌等病原菌，被蚊虫叮咬容易导致细菌的感染，严重影响我们的工作和生活。目前，市面上针对蚊虫叮咬所导致的炎症及细菌感染最常见的治疗方法一般为糖皮质激素类及抗组胺类等化学药物治疗，其副作用较大，安全性较低。
在近年来越来越注重产品安全性的情况下，市面上也出现了一系列的天然植物抑菌制剂。但由于植物抑菌剂的抗菌作用有限等原因，大部分产品仍然添加了醋酸氯乙定等化学合成的抑菌剂。另外还有一系列的皮肤抑菌软膏，虽未使用合成抑菌剂，但仍添加了化学合成的防腐剂，如尼泊金乙酯及可能会导致皮肤刺激性的十二烷基硫酸钠等。
发明内容
为解决现有技术中存在的天然植物抑菌成分抑菌效果不佳，产品添加人工合成抑菌剂及防腐剂而易导致产生过敏现象等问题，本发明提供了一种含艾叶提取物的天然植物抑菌软膏。该抑菌软膏的组分为天然来源，不含化学合成抑菌剂和防腐剂，安全、无毒、无刺激，且抑菌、止痒等作用效果明显。
本发明还提供了上述所述含艾叶提取物的天然植物抑菌软膏的制备方 法。
一种含艾叶提取物的天然植物抑菌软膏，按重量百分比，包括如下组分：
艾叶提取物16-34％，马齿苋提取物1-5％，苦参根粉0.03-0.1％，甘草酸二钾0.1-0.5％，山茶籽油2-10％，薄荷脑0.5-2％，戊二醇1-3％，薰衣草精油0.1-1％，牡丹皮提取物0.5-2％，乳化剂2-5％，增稠剂0.5-2％，水35.4-76.2％；
所述艾叶提取物包括艾草纯露、艾叶蜡质和艾叶重油。
优选的，按重量百分比，所述天然植物抑菌软膏中含：艾草纯露15～30％，艾叶蜡质0.5％～1％，艾叶重油1％～2％。
优选的，按重量百分比，包括如下组分：
艾叶提取物22.5％，马齿苋提取物2％，苦参根粉0.05％，甘草酸二钾0.2％，山茶籽油8％，薄荷脑1％，戊二醇3％，薰衣草精油0.2％，牡丹皮提取物1％，乳化剂5％，增稠剂1.2％，水56.6％；
所述艾叶提取物包括艾草纯露、艾叶蜡质和艾叶重油；且按重量百分比，所述天然植物抑菌软膏中含：艾草纯露20％，艾叶蜡质0.5％，艾叶重油2％。
优选的，所述艾草纯露由如下方法制取得到：
取新鲜艾草全草，清洗干净，并进行小段化处理，进行水蒸气蒸馏，冷凝，得到所述艾草纯露。
优选的，所述艾叶蜡质以及所述艾叶重油由如下方法制取得到：
(1)将新鲜艾草的艾叶阴干至含水量≤8％，粉碎，过20-100目筛，得到艾叶粗粉；
(2)将所述艾叶粗粉依次采用超临界CO
2萃取装置进行萃取、超临界CO
2精馏柱分离、分离釜分离纯化，得到艾叶浸膏；
(3)将所述艾叶浸膏进行分子蒸馏，得到所述艾叶蜡质和所述艾叶重油。
优选的，所述增稠剂包括阿拉伯胶或黄原胶。
优选的，所述的乳化剂包括天然来源的鲸蜡硬脂醇、椰油基葡糖苷、花生醇、山嵛醇或花生醇葡糖苷。
制备上述任一项所述的一种含艾叶提取物的天然植物抑菌软膏的方法，包括如下步骤：
(1)按照所述重量百分比，称取艾草纯露、马齿苋提取物、苦参根粉、甘草酸二钾、戊二醇以及增稠剂，并溶于水中，混合均匀，加热至70-90℃并保温，得到水相；
(2)按照所述重量百分比，称取艾叶蜡质、山茶籽油以及乳化剂，加热至70-90℃溶解并保温，得到油相；
(3)将步骤(1)的水相加入至步骤(2)的油相中，边加边搅拌，混合均匀后，均质，冷却，得到软膏基质；
(4)按照所述重量百分比，称取艾叶重油、薄荷脑、薰衣草精油以及牡丹皮提取物，混合均匀后，加入至步骤(3)的软膏基质中，搅拌混合均匀，冷却至室温，得到所述含艾叶提取物的天然植物抑菌软膏。
优选的，步骤(1)、(2)中，所述加热均为加热至80℃；所述保温的时间均为30-35min。
优选的，步骤(3)中，所述均质的转速为1500-3000r/min，时间为30min，且均质过程中保持温度在60℃以上。
优选的，步骤(3)中，所述冷却为自然冷却至40℃。
优选的，步骤(4)中，所述搅拌的转速为800-1200r/min，时间为10-20min。
与现有技术相比，本发明具有如下优点和有益效果：
(1)本发明的含艾叶提取物的天然植物抑菌软膏中，主要包含有艾叶提取物、马齿苋提取物、苦参根粉、甘草酸二钾、山茶籽油、薄荷脑、薰衣草精油以及牡丹皮提取物等多种高效的抑菌消炎组分，且这些组分均为天然来源；从而，使本发明的含艾叶提取物的天然植物抑菌软膏具有明显的抑菌、消炎、镇痛、止痒的效果，对大肠杆菌以及金黄色葡萄球菌均具有明显的抑制效果，且具有护肤作用，并且不含化学合成抑菌剂和防腐剂，绿色、安全、无毒、无刺激。
(2)本发明的制备方法中，通过严格控制水相及油相的混合温度、均质温度及均质时间，从而制备得到质地均匀、油相-水相结合良好的软膏，方便于涂覆使用，并提高使用的体验感。
以下结合具体实施例对本发明的技术方案作进一步详细的描述，但这些实施例仅用于例证的目的，本发明的保护范围及实施方式不限于此。
本发明的含艾叶提取物的天然植物抑菌软膏，按重量百分比，包括如下组分：
艾叶提取物16-34％，马齿苋提取物1-5％，苦参根粉0.03-0.1％，甘草酸二钾0.1-0.5％，山茶籽油2-10％，薄荷脑0.5-2％，戊二醇1-3％，薰衣草精油0.1-1％，牡丹皮提取物0.5-2％，乳化剂2-5％，增稠剂0.5-2％，水35.4-76.2％；
其中，所述艾叶提取物包括艾草纯露、艾叶蜡质和艾叶重油。并且，具体的实施方式中，按重量百分比，所述天然植物抑菌软膏中含：艾草纯露15～30％，艾叶蜡质0.5％～1％，艾叶重油1％～2％。
艾叶作为传统中药，是菊科蒿属植物艾的干燥叶，其提取物具有杀菌、 抑菌的功效；马齿苋提取物具有抗刺激、抗过敏和抗炎的活性成分；苦参中含有的苦参碱和氧化苦参碱均被证明具有抗菌消炎的活性；甘草酸二钾具有抗炎、抗过敏、保湿的功效；山茶籽油对肌肤有很好的保健作用，还具有杀菌解毒和加速伤口愈合等功效；薄荷脑作为常用的消炎镇痛成分，常用于蚊虫叮咬的制剂中；薰衣草精油以及牡丹皮提取物均具有抑菌效果，而戊二醇则起到协同增效作用。
在本发明的含艾叶提取物的天然植物抑菌软膏中，主要包含有艾叶提取物、马齿苋提取物、苦参根粉、甘草酸二钾、山茶籽油、薄荷脑、薰衣草精油以及牡丹皮提取物等多种高效的抑菌消炎组分，且这些组分均为天然来源，无毒、无刺激；从而，使本发明的含艾叶提取物的天然植物抑菌软膏具有明显的抑菌、消炎、镇痛、止痒的效果，对大肠杆菌及金黄色葡萄球菌均具有明显的抑制效果，能用于蚊虫叮咬部位的消毒、止痒，且具有护肤作用，并且不含化学合成抑菌剂和防腐剂，绿色、安全、无毒、无刺激。
实施例1
一种含艾叶提取物的天然植物抑菌软膏，按重量百分比计，包含组分如下：
艾草纯露20％，艾叶蜡质0.5％，艾叶重油2％，马齿苋提取物2％，苦参根粉0.05％，甘草酸二钾0.2％，山茶籽油8％，薄荷脑1％，戊二醇3％，薰衣草精油0.2％，牡丹皮提取物1％，鲸蜡硬脂醇5％，阿拉伯胶1.2％，余量为水。
其中，所述艾草纯露由如下方法制取得到：
取新鲜艾草全草，清洗干净，剪切成小段，进行水蒸气蒸馏，冷凝，得到所述艾草纯露。
所述艾叶蜡质以及所述艾叶重油由如下方法制取得到：
(1)将新鲜艾草的艾叶阴干至含水量为7％，粉碎，过50目筛，得到艾叶粗粉；
(2)将所述艾叶粗粉依次采用超临界CO
2萃取装置进行萃取、超临界CO
2精馏柱分离、分离釜分离纯化，得到艾叶浸膏；
(3)将所述艾叶浸膏进行分子蒸馏，得到所述艾叶蜡质和所述艾叶重油。
制备本实施例的含艾叶提取物的天然植物抑菌软膏，步骤如下：
(1)按重量百分比，称取艾草纯露、马齿苋提取物、苦参根粉、甘草酸二钾、戊二醇、阿拉伯胶，溶于水中，混合均匀，80℃保温30min，作为水相；
(2)按重量百分比，称取艾叶蜡质、山茶籽油及鲸蜡硬脂醇，80℃加热溶解，保温30min，作为油相；
(3)将水相加入油相中，边加边搅拌，混合均匀后，2500r/min均质30min，温度保持60℃以上，缓慢冷却至40℃，得软膏基质；
(4)按重量百分比，称取艾叶重油、薄荷脑、薰衣草精油以及牡丹皮提取物，混合均匀后，加入软膏基质中，混合，800r/min转速搅拌10min，冷却至室温，即得到本实施例的含艾叶提取物的天然植物抑菌软膏。
实施例2
一种含艾叶提取物的天然植物抑菌软膏，按重量百分比计，包含组分如下：
艾草纯露15％，艾叶蜡质1％，艾叶重油1％，马齿苋提取物2％，苦参根粉0.05％，甘草酸二钾0.2％，山茶籽油10％，薄荷脑1％，戊二醇3％， 薰衣草精油0.2％，牡丹皮提取物0.5％，椰油基葡糖苷2％，黄原胶1.2％，余量为水。
其中，所述艾草纯露由如下方法制取得到：
取新鲜艾草全草，清洗干净，剪切成小段，进行水蒸气蒸馏，冷凝，得到所述艾草纯露。
所述艾叶蜡质以及所述艾叶重油由如下方法制取得到：
(1)将新鲜艾草的艾叶阴干至含水量为6％，粉碎，过30目筛，得到艾叶粗粉；
(2)将所述艾叶粗粉依次采用超临界CO
2萃取装置进行萃取、超临界CO
2精馏柱分离、分离釜分离纯化，得到艾叶浸膏；
(3)将所述艾叶浸膏进行分子蒸馏，得到所述艾叶蜡质和所述艾叶重油。
制备本实施例的含艾叶提取物的天然植物抑菌软膏，步骤如下：
(1)按重量百分比，称取艾草纯露、马齿苋提取物、苦参根粉、甘草酸二钾、戊二醇、黄原胶，溶于水中，混合均匀，70℃保温30min，作为水相；
(2)按重量百分比，称取艾叶蜡质、山茶籽油及椰油基葡糖苷，70℃加热溶解，保温30min，作为油相；
(3)将水相加入油相中，边加边搅拌，混合均匀后，2500r/min均质30min，温度保持60℃以上，缓慢冷却至40℃，得软膏基质；
(4)按重量百分比，称取艾叶重油、薄荷脑、薰衣草精油以及牡丹皮提取物，混合均匀后，加入软膏基质中，混合，800r/min转速搅拌15min，冷却至室温，即得到本实施例的含艾叶提取物的天然植物抑菌软膏。
实施例3
一种含艾叶提取物的天然植物抑菌软膏，按重量百分比计，包含组分如 下：
艾草纯露30％，艾叶蜡质0.5％，艾叶重油2％，马齿苋提取物3％，苦参根粉0.05％，甘草酸二钾0.2％，山茶籽油8％，薄荷脑2％，戊二醇3％，薰衣草精油0.2％，牡丹皮提取物0.8％，鲸蜡硬脂醇2％，黄原胶1.2％，余量为水。
其中，所述艾草纯露由如下方法制取得到：
取新鲜艾草全草，清洗干净，剪切成小段，进行水蒸气蒸馏，冷凝，得到所述艾草纯露。
所述艾叶蜡质以及所述艾叶重油由如下方法制取得到：
(1)将新鲜艾草的艾叶阴干至含水量为7％，粉碎，过40目筛，得到艾叶粗粉；
(2)将所述艾叶粗粉依次采用超临界CO
2萃取装置进行萃取、超临界CO
2精馏柱分离、分离釜分离纯化，得到艾叶浸膏；
(3)将所述艾叶浸膏进行分子蒸馏，得到所述艾叶蜡质和所述艾叶重油。
制备本实施例的含艾叶提取物的天然植物抑菌软膏，步骤如下：
(1)按重量百分比，称取艾草纯露、马齿苋提取物、苦参根粉、甘草酸二钾、戊二醇、黄原胶，溶于水中，混合均匀，90℃保温30min，作为水相；
(2)按重量百分比，称取艾叶蜡质、山茶籽油及鲸蜡硬脂醇，90℃加热溶解，保温30min，作为油相；
(3)将水相加入油相中，边加边搅拌，混合均匀后，2500r/min均质30min，温度保持60℃以上，缓慢冷却至40℃，得软膏基质；
(4)按重量百分比，称取艾叶重油、薄荷脑、薰衣草精油以及牡丹皮提取物，混合均匀后，加入软膏基质中，混合，800r/min转速搅拌20min，冷却至室温，即得到本实施例的含艾叶提取物的天然植物抑菌软膏。
二、天然植物抑菌软膏的抑菌效果测试
测试菌：
大肠杆菌ATCC 25922或8099；
金黄色葡萄球菌ATCC 6538；
以上菌株均购置于广东省微生物研究所。
测试方法：
取实施例1-3制得的抑菌软膏，按《GB 15979-2002一次性使用卫生用品卫生标准》附录C4的方法进行抑菌性能测试，试验结果如表1所示。
表1实施例1-3的抑菌软膏的抑菌性能测试结果
试验结果表明，实施例1-3制得的抑菌软膏对大肠杆菌的抑菌率均大于90％,有较强抑菌作用；对金黄色葡萄球菌的抑菌率，实施例1的抑菌软膏的抑菌率大于90％，实施例2及实施例3的抑菌软膏的抑菌率均大于50％而小于90％，表明具有抑菌作用。
三、天然植物抑菌软膏的皮肤变态反应测试
对本发明实施例1制得的抑菌软膏按《消毒技术规范(2002年版)》皮肤变态反应检查法进行检测，未见皮肤变态反应。具体检测内容如下：
试验方法：选取48只豚鼠，根据体重随机分为3组(阴性对照组、阳性对照组和实验组)，并于试验前24h对其背部样品敷贴部位进行3cm*3cm去毛处理。于D0、D7、D14天以0.5ml/只动物的剂量将诱导剂敷贴于背部左侧2cm*2cm的区域，以一层称量纸覆盖，再以无刺激胶带封闭固定6h后移除并清洗；最后于D28天以相应的激发剂对动物进行激发。
评分标准：如表2所示。
表2皮肤反应试验评分标准
致敏强度分级标准：致敏率为0％时可视为未见皮肤变态反应，0％～8％为极轻度致敏，9％～28％为轻度致敏，29％～64％为中度致敏，65％～80％致敏程度为强度，80％～100％致敏程度为极强度。
实验结果：如表3所示。
表3皮肤变态反应测试结果
由表3的试验结果表明，与阳性对照组比，阴性对照组和实验组动物在诱导激发后均未出现红斑或水肿等皮肤变态反应现象，表明本发明制备的抑菌软膏安全无刺激。
四、疗效评价
取实施例1制备的抑菌软膏对蚂蚁、蚊虫叮咬后的皮肤及时涂抹擦拭，可即时镇静止痒止痛，褪除红肿、丘疱疹等症状。4人测试，显效率达100％。表 明本发明制备的抑菌软膏具有潜在的消炎止痒作用。
以上实施例仅为本发明的较优实施例，仅在于对本发明技术方案作进一步详细描述，用于帮助理解本发明技术方案的原理。同时，对于本领域的一般技术人员，依据本发明技术方案，在具体实施方式以及应用范围上均会有改变之处，综上所述，任何未脱离本发明原理及其精神实质下所做的变更、组合、删除、替换或修改等均将包含在本发明的保护范围内。
Claims (10)
- 一种含艾叶提取物的天然植物抑菌软膏，其特征在于，按重量百分比，包括如下组分：艾叶提取物16-34％，马齿苋提取物1-5％，苦参根粉0.03-0.1％，甘草酸二钾0.1-0.5％，山茶籽油2-10％，薄荷脑0.5-2％，戊二醇1-3％，薰衣草精油0.1-1％，牡丹皮提取物0.5-2％，乳化剂2-5％，增稠剂0.5-2％，水35.4-76.2％；所述艾叶提取物包括艾草纯露、艾叶蜡质和艾叶重油。
- 根据权利要求1所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，按重量百分比，所述天然植物抑菌软膏中含：艾草纯露15～30％，艾叶蜡质0.5％～1％，艾叶重油1％～2％。
- 根据权利要求1所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，按重量百分比，包括如下组分：艾叶提取物22.5％，马齿苋提取物2％，苦参根粉0.05％，甘草酸二钾0.2％，山茶籽油8％，薄荷脑1％，戊二醇3％，薰衣草精油0.2％，牡丹皮提取物1％，乳化剂5％，增稠剂1.2％，水56.6％；所述艾叶提取物包括艾草纯露、艾叶蜡质和艾叶重油；且按重量百分比，所述天然植物抑菌软膏中含：艾草纯露20％，艾叶蜡质0.5％，艾叶重油2％。
- 根据权利要求1～3任一项所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，所述艾草纯露由如下方法制取得到：取新鲜艾草全草，清洗干净，并进行小段化处理，进行水蒸气蒸馏，冷凝，得到所述艾草纯露。
- 根据权利要求1～3任一项所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，所述艾叶蜡质以及所述艾叶重油由如下方法制取得到：(1)将新鲜艾草的艾叶阴干至含水量≤8％，粉碎，过20-100目筛，得到艾叶粗粉；(2)将所述艾叶粗粉依次采用超临界CO 2萃取装置进行萃取、超临界CO 2精馏柱分离、分离釜分离纯化，得到艾叶浸膏；(3)将所述艾叶浸膏进行分子蒸馏，得到所述艾叶蜡质和所述艾叶重油。
- 根据权利要求1～3任一项所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，所述增稠剂包括阿拉伯胶或黄原胶。
- 根据权利要求1～3任一项所述的一种含艾叶提取物的天然植物抑菌软膏，其特征在于，所述的乳化剂包括天然来源的鲸蜡硬脂醇、椰油基葡糖苷、花生醇、山嵛醇或花生醇葡糖苷。
- 制备权利要求1～7任一项所述的一种含艾叶提取物的天然植物抑菌软膏的方法，其特征在于，包括如下步骤：(1)按照所述重量百分比，称取艾草纯露、马齿苋提取物、苦参根粉、甘草酸二钾、戊二醇以及增稠剂，并溶于水中，混合均匀，加热至70-90℃并保温，得到水相；(2)按照所述重量百分比，称取艾叶蜡质、山茶籽油以及乳化剂，加热至70-90℃溶解并保温，得到油相；(3)将步骤(1)的水相加入至步骤(2)的油相中，边加边搅拌，混合均匀后，均质，冷却，得到软膏基质；(4)按照所述重量百分比，称取艾叶重油、薄荷脑、薰衣草精油以及牡丹皮提取物，混合均匀后，加入至步骤(3)的软膏基质中，搅拌混合均匀， 冷却至室温，得到所述含艾叶提取物的天然植物抑菌软膏。
- 根据权利要求8所述的制备方法，其特征在于，步骤(1)、(2)中，所述加热均为加热至80℃；所述保温的时间均为30-35min。
- 根据权利要求8所述的制备方法，其特征在于，步骤(3)中，所述均质的转速为1500-3000r/min，时间为30min，且均质过程中保持温度在60℃以上；所述冷却为自然冷却至40℃。
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