WO2021114085A1 - 可固定的细胞培养组件 - Google Patents

可固定的细胞培养组件 Download PDF

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WO2021114085A1
WO2021114085A1 PCT/CN2019/124299 CN2019124299W WO2021114085A1 WO 2021114085 A1 WO2021114085 A1 WO 2021114085A1 CN 2019124299 W CN2019124299 W CN 2019124299W WO 2021114085 A1 WO2021114085 A1 WO 2021114085A1
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cell culture
tray
bag
biological
chuck
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PCT/CN2019/124299
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English (en)
French (fr)
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张豪
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苏州生动细胞生物科技有限公司
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Priority to PCT/CN2019/124299 priority Critical patent/WO2021114085A1/zh
Publication of WO2021114085A1 publication Critical patent/WO2021114085A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

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  • This application relates to the field of cell culture technology, and in particular to a fixing assembly of a cell culture bag.
  • the cell culture bag is a disposable device when culturing cells.
  • liquid or gas such as cell fluid, culture fluid, nutrient solution into the cell culture bag or withdraw from the culture bag, and place the cell culture bag
  • the biological shaker usually includes a shaker body and a tray set on the body.
  • the existing cell culture bag does not have a fixed connection device with the tray, but directly places the culture bag on the tray.
  • the tray is provided with components such as pressure rods or press blocks, and the cell culture bag is fixed on the tray by pressing the edge of the cell culture bag. To avoid sliding of the cell culture bag.
  • the culture bag When the culture bag is inflated, the culture bag bulges, and the contact surface between the bottom of the culture bag and the tray is still partially suspended.
  • the purpose of this application is to overcome the problem that the existing cell culture bag cannot be detachably fixedly connected to the shaker, which causes the contact surface between the bottom of the cell culture bag and the tray to be partially suspended, and to provide a fixable cell culture assembly, including A cell culture bag and a biological shaker;
  • the biological shaker includes a body and a tray provided on the body;
  • the cell culture bag includes a closed bag body and a fixing assembly provided at the lower end of the closed bag body for connecting with the tray;
  • the fixing assembly includes a bottom sheet, a chuck and a screw cap; the bottom sheet is fixedly connected to the closed bag body; the chuck includes a threaded post vertically fixed to the lower end of the bottom sheet and a plurality of elastic clamping jaws arranged at the lower end of the threaded post; There is a space between the plurality of elastic clamping jaws, and the plurality of elastic clamping jaws are arranged in a hollow truncated cone shape; the tightening cap has a through structure up and down, and includes a threaded pipe section matched with a threaded column and a threaded pipe section arranged at one end of the threaded pipe section. For the tapered pipe section where the elastic clamping jaws are locked;
  • the upper end surface of the tray is provided with a groove, and the bottom of the groove is vertically provided with a clamping rod matched with the clamping head.
  • the threaded column is hollow.
  • the depth of the groove is greater than or equal to the height of the fixing component.
  • the closed bag body is a closed bag cavity formed by bending a single bag sheet and hot pressing, or a closed bag cavity formed by laminating two bag sheets and hot pressing.
  • the beneficial effect of the present application is that by arranging a clamping rod on the tray of the biological shaker, and arranging a fixing component for clamping and cooperating with the clamping rod on the cell culture bag, the cell culture bag and the tray can be quickly fixed and separated.
  • the cell culture bag will not slide when swinging, the cell culture efficiency is higher, the loss is smaller, and the improvement of the tray is small. It can be applied to most biological shakers on the market, reduces the replacement cost of shakers, and is easy to promote.
  • FIG. 1 is a schematic diagram of the three-dimensional structure of the fixing assembly of the fixable cell culture assembly of the present application.
  • Fig. 2 is a schematic diagram of the front view of Fig. 1.
  • FIG. 3 is a schematic cross-sectional view of the structure of FIG. 2.
  • Fig. 4 is a schematic diagram of the structure of the chuck and the screw cap of Fig. 2 separated.
  • FIG. 5 is a schematic top view of the structure of the tray of the fixable cell culture assembly of the present application.
  • Fig. 6 is a schematic diagram of the structure of the cell culture bag of this application installed on the tray of the biological shaker through the fixing component.
  • the fixable cell culture components shown in Figures 1 to 6 include cell culture bags and biological shakers; the biological shakers include a body and a tray 5 set on the body.
  • the biological shakers can be directly purchased in the market, and only The tray 5 needs to be improved accordingly.
  • the biological shaker can be of the structure described in the Chinese invention patent with publication number CN107365692A; the cell culture bag includes a closed bag body 1 and a lower end of the closed bag body 1 for communication with The fixed component connected to the tray 5.
  • the fixing assembly includes the negative film 2, the chuck 3 and the tightening cap 4.
  • the upper end of the tray 5 is provided with a groove 6.
  • the number of fixing components is the same as that of the groove 6. The number remains the same.
  • the grooves 6 are preferably designed with three three-points, so that the cell culture bag will be more firmly fixed; the bottom of the groove 6 is vertically provided with a clamping rod 7 that matches the chuck 3; the bottom sheet 2 and the closed bag
  • the body 1 is fixedly connected;
  • the chuck 3 includes a threaded column 3.1 vertically fixed to the lower end of the film 2 and a plurality of elastic clamping jaws 3.2 arranged at the lower end of the threaded column 3.1; in order to fix the cell culture bag tighter and avoid its shaking, the threaded column 3.1 It can be set to be hollow to facilitate insertion of the clamping rod 7 into the threaded column 3.1.
  • the hollow in the threaded column 3.1 is preferably a vertical cavity with a diameter greater than or equal to the diameter of the clamping rod 7.
  • the plurality of elastic clamping jaws 3.2 are spaced apart, and the plurality of elastic clamping jaws 3.2 are arranged in a hollow truncated cone shape; there are three or four elastic clamping jaws 3.2, and the structure of the elastic clamping jaws 3.2 is shown in Figure 1 or 3 or 4.
  • the elastic clamping jaw 3.2 there are four elastic clamping jaws 3.2, and the four elastic clamping jaws 3.2 are evenly arranged along the circumferential direction of the threaded column 3.1; the lower ends of the plurality of elastic clamping jaws 3.2 are gathered together and reserved in the middle for the clamping rod 7 to pass
  • the elastic clamping jaw 3.2 is preferably made of plastic or rubber material with a certain degree of plasticity; as shown in Figure 3, when it is squeezed from the outside to the inside, it is squeezed by the tightening cap 4, and the elastic clamping jaw 3.2 Collapse to realize the clamping of the clamping rod 7;
  • the tightening cap 4 is an upper and lower through structure, which includes a threaded pipe section 4.1 matched with the threaded column 3.1 and a tapered pipe section 4.2 provided at one end of the threaded pipe section 4.1 for locking the elastic clamping jaw 3.2 .
  • the depth of the groove 6 should be greater than or equal to the height of the fixed component, so that after the cell culture bag is fixed on the tray 5 by the fixed component, the fixed component of the cell culture bag will not rise upward. When the tray 5 is shaken, the liquid in the cell culture bag can flow smoothly, which is beneficial to cell culture.
  • the notch of the groove 6 should be provided for tools or fingers to enter the groove 6 so as to rotate and tighten the cap 4.
  • the tray 5 and the body of the biological shaker are detachably connected, so the cell culture assembly of this embodiment actually only needs to improve the existing tray 5, and stick or weld the bottom sheet 2 of the fixing assembly to the existing This improvement greatly reduces the cost for users to replace products, and only needs to purchase corresponding fixed components and trays 5.
  • the closed bag body 1 may be a closed bag cavity formed by bending a single bag sheet and hot pressing, or may be a closed bag cavity formed by laminating two bag sheets and hot pressing. When formed from a single piece of bag sheet, it belongs to a small-volume cell culture bag and only requires one fixed component.

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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Abstract

本申请公开了可固定的细胞培养组件，包括细胞培养袋和生物摇床；生物摇床包括托盘；细胞培养袋包括封闭袋体和固定组件；固定组件包括底片、夹头和旋紧帽；底片与封闭袋体固定连接；夹头包括螺纹柱及弹性夹爪；旋紧帽为上下贯通结构，其包括螺纹管段和锥管段；托盘上端面设有凹槽，凹槽底部竖直设有与夹头配合的夹杆。本申请的有益效果是：实现了细胞培养袋与托盘的快速固定和分离，托盘摆动时细胞培养袋不会滑动，细胞培养效率更高，损失更小，且对托盘改进较小，可适用于市场上大多数生物摇床，降低了摇床更换成本，易于推广。

Description

可固定的细胞培养组件 技术领域
本申请涉及细胞培养技术领域，尤其地涉及一种细胞培养袋的固定组件。
背景技术
细胞培养袋在培养细胞时是一次性的装置，进行细胞培养时，需要将细胞液、培养液、营养液等液体或气体注入细胞培养袋内或从培养袋内抽出，并将细胞培养袋置于生物摇床上，通过生物摇床的摇摆促进细胞培养；生物摇床通常包括摇床机体和设于机体上的托盘。现有的细胞培养袋没有与托盘固定的连接装置，而是直接将培养袋置于托盘上，在托盘上设置有压杆或压块等部件，通过将细胞培养袋的边缘压住固定在托盘上，避免细胞培养袋的滑动。当对培养袋充气时，培养袋鼓起，培养袋底部和托盘的接触面仍然会有部分悬空。
发明内容
本申请的目的在于克服现有细胞培养袋无法与摇床可拆式固定连接，导致细胞培养袋底部和托盘的接触面仍然会有部分悬空的问题，提供一种可固定的细胞培养组件，包括细胞培养袋和生物摇床；所述生物摇床包括机体和设于机体上的托盘；所述细胞培养袋包括封闭袋体和设于封闭袋体下端的用于与托盘连接的固定组件；
所述固定组件包括底片、夹头和旋紧帽；所述底片与封闭袋体固定连接；所述夹头包括垂直固定于底片下端的螺纹柱及设于螺纹柱下端的多个弹性夹爪；多个弹性夹爪之间设有间距，且多个弹性夹爪排列为空心圆台状；所述旋紧帽为上下贯通结构，其包括与螺 纹柱配合的螺纹管段和设于螺纹管段一端的用于锁紧弹性夹爪的锥管段；
所述托盘上端面设有凹槽，所述凹槽底部竖直设有与夹头配合的夹杆。
进一步，所述弹性夹爪有三个或四个，沿所述螺纹柱周向均匀布置；多个弹性夹爪的下端相聚拢并在中部预留有供所述夹杆通过的开口。
进一步，所述螺纹柱为空心。
进一步，所述凹槽深度大于或等于所述固定组件的高度。
进一步，所述封闭袋体是由单块袋片折弯后热压形成的一个封闭袋腔或由两块袋片层叠后热压形成的一个封闭袋腔。
本申请的有益效果是：通过在生物摇床的托盘上设置夹杆，并在细胞培养袋上设置与夹杆夹持配合的固定组件，实现了细胞培养袋与托盘的快速固定和分离，托盘摆动时细胞培养袋不会滑动，细胞培养效率更高，损失更小，且对托盘改进较小，可适用于市场上大多数生物摇床，降低了摇床更换成本，易于推广。
附图说明
图1为本申请可固定的细胞培养组件的固定组件的立体结构示意图。
图2为图1的主视结构示意图。
图3为图2的剖视结构示意图。
图4为图2的夹头和旋紧帽分离的结构示意图。
图5为本申请可固定的细胞培养组件的托盘的俯视结构示意图。
图6为本申请的细胞培养袋通过固定组件安装在生物摇床的托盘上的结构示意图。
图中，1.封闭袋体；2.底片；3.夹头；3.1.螺纹柱；3.2.弹性夹爪；4.旋紧帽；4.1.螺纹管段；4.2.锥管段；5.托盘；6.凹槽；7.夹杆。
具体实施方式
以下结合附图和具体实施例对本申请作进一步的详细描述。
如图1～6所示的可固定的细胞培养组件，包括细胞培养袋和生物摇床；生物摇床包括机体和设于机体上的托盘5，生物摇床可以是市场上直接购买获得，只需要对托盘5进行相应改进即可，比如，生物摇床可以是公开号为CN107365692A的中国发明专利所述的结构；细胞培养袋包括封闭袋体1和设于封闭袋体1下端的用于与托盘5连接的固定组件。
固定组件包括底片2、夹头3和旋紧帽4；托盘5上端面设有凹槽6，本实施例中凹槽6如图5所示设置有2个，固定组件的数量与凹槽6数量保持一致，当然凹槽6优选为呈三点式设计三个，这样细胞培养袋会被固定的更牢固；凹槽6底部竖直设有与夹头3配合的夹杆7；底片2与封闭袋体1固定连接；夹头3包括垂直固定于底片2下端的螺纹柱3.1及设于螺纹柱3.1下端的多个弹性夹爪3.2；为了细胞培养袋固定的更紧，避免其晃动，螺纹柱3.1可设置为空心以便于夹杆7插入螺纹柱3.1内，因此螺纹柱3.1内的空心优选为直径大于或等于夹杆7直径的竖直空腔。多个弹性夹爪3.2之间设有间距，且多个弹性夹爪3.2排列为空心圆台状；弹性夹爪3.2有三个或四个，弹性夹爪3.2的结构如图1或3或4所示，本实施例中弹性夹爪3.2为四个，四个弹性夹爪3.2沿螺纹柱3.1周向均匀布置；多个弹性夹爪3.2的下端相聚拢并在中部预留有供夹杆7通过的开口，弹性夹爪3.2优选为塑料材质或橡胶材质，具有一定的塑性；如图3所示，当受到由外至内的挤压时，即受到旋 紧帽4的挤压，弹性夹爪3.2收拢实现对夹杆7的夹紧；旋紧帽4为上下贯通结构，其包括与螺纹柱3.1配合的螺纹管段4.1和设于螺纹管段4.1一端的用于锁紧弹性夹爪3.2的锥管段4.2。固定时，如图6所示，首先将旋紧帽4套设在夹杆7外，再将夹头3套设在夹杆7上且夹头3处于旋紧帽4内，转动旋紧帽4使其不断上移并逐渐将夹头3夹紧，最终旋紧帽4将夹头3夹紧，夹头3将夹杆7夹紧后为如图6所示的状态，此时，细胞培养袋通过固定组件固定在托盘5上。
为了使细胞培养袋平放，凹槽6深度应该大于或等于固定组件的高度，从而细胞培养袋通过固定组件固定在托盘5上后，细胞培养袋的固定组件处不会朝上顶起，当托盘5摇晃时，细胞培养袋内的液体可平顺的流动，利于细胞培养。另外，凹槽6的槽口应该设置的便于工具或手指进入凹槽6内，以便转动旋紧帽4。托盘5与生物摇床的机体是可分离式连接的，因此本实施例的细胞培养组件实际上只需要对现有的托盘5进行改进，并将固定组件的底片2粘贴或焊接固定在现有的细胞培养袋上即可，这种改进大大降低了用户更换产品所需的成本，只需要购买相应的固定组件和托盘5即可。
本实施例中封闭袋体1可以是由单块袋片折弯后热压形成的一个封闭袋腔，也可以是由两块袋片层叠后热压形成的一个封闭袋腔。当由单块袋片热压成型时，其属于小体积细胞培养袋，只需要一个固定组件即可。

Claims (5)

  1. 一种可固定的细胞培养组件，包括细胞培养袋和生物摇床；所述生物摇床包括机体和设于机体上的托盘(5)；其特征在于：所述细胞培养袋包括封闭袋体(1)和设于封闭袋体(1)下端的用于与托盘(5)连接的固定组件；
    所述固定组件包括底片(2)、夹头(3)和旋紧帽(4)；所述底片(2)与封闭袋体(1)固定连接；所述夹头(3)包括垂直固定于底片(2)下端的螺纹柱(3.1)及设于螺纹柱(3.1)下端的多个弹性夹爪(3.2)；多个弹性夹爪(3.2)之间设有间距，且多个弹性夹爪(3.2)排列为空心圆台状；所述旋紧帽(4)为上下贯通结构，其包括与螺纹柱(3.1)配合的螺纹管段(4.1)和设于螺纹管段(4.1)一端的用于锁紧弹性夹爪(3.2)的锥管段(4.2)；
    所述托盘(5)上端面设有凹槽(6)，所述凹槽(6)底部竖直设有与夹头(3)配合的夹杆(7)。
  2. 根据权利要求1所述的可固定的细胞培养组件，其特征在于：所述弹性夹爪(3.2)有三个或四个，沿所述螺纹柱(3.1)周向均匀布置；多个弹性夹爪(3.2)的下端相聚拢并在中部预留有供所述夹杆(7)通过的开口。
  3. 根据权利要求1所述的可固定的细胞培养组件，其特征在于：所述螺纹柱(3.1)为空心。
  4. 根据权利要求1所述的可固定的细胞培养组件，其特征在于：所述凹槽(6)深度大于或等于所述固定组件的高度。
  5. 根据权利要求1所述的可固定的细胞培养组件，其特征在于：所述封闭袋体(1)是由单块袋片折弯后热压形成的一个封闭袋腔或由两块袋片层叠后热压形成的一个封闭袋腔。
PCT/CN2019/124299 2019-12-10 2019-12-10 可固定的细胞培养组件 WO2021114085A1 (zh)

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Cited By (1)

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CN115029226A (zh) * 2022-08-12 2022-09-09 北京汉氏联合生物技术股份有限公司 免疫细胞规模化智能培养装置

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CN105567565A (zh) * 2016-03-08 2016-05-11 上海吉凯基因科技有限公司 一种连续细胞培养摇床
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