WO2021104500A1

WO2021104500A1 - Sample analyzer and laser assembly

Info

Publication number: WO2021104500A1
Application number: PCT/CN2020/132466
Authority: WO
Inventors: 于记良
Original assignee: 深圳市帝迈生物技术有限公司
Priority date: 2019-11-30
Filing date: 2020-11-27
Publication date: 2021-06-03
Also published as: CN112881720B; CN112881720A

Abstract

A sample analyzer and a laser assembly (300). The laser assembly (300) comprises a light source base (320), a laser fixing plate (330), a laser (340) and a locking plate (350); the light source base (320) is provide with an assembling cavity (322); the laser fixing plate (330) is adjustably embedded in the assembling cavity (322) in B and C directions; the laser (340) is embedded in the laser fixing plate (330) and is used for emitting lasers towards A direction; and a laser fixing plate locking member (333) is used to adjust and lock the laser fixing plate (330).

Description

Sample analyzer, laser assembly

Technical field

This application relates to the technical field of medical detection and analysis, in particular to a sample analyzer and a laser assembly.

Background technique

Automatic analyzers have been widely used in hospitals, medical testing laboratories, and regional testing centers at all levels due to their fast measurement speed, high accuracy, and small consumption of reagents.

The automatic analyzer is equipped with a detection device, and the detection device detects the sample in the optical detection cell by optical detection.

However, the optical detection components in the existing detection devices usually have a large structure, which restricts the miniaturization and integration of the whole machine, and is not easy to adjust, and it is prone to jamming during adjustment.

Summary of the invention

The present application provides a sample analyzer and a laser assembly to solve the technical problem that the optical detection assembly in the prior art usually has a large structure, which restricts the miniaturization and integration of the whole machine, and is inconvenient to adjust, and is prone to jam during adjustment.

In order to solve the above technical problems, a technical solution adopted in this application is to provide a laser assembly, which includes:

Light source base with assembly cavity;

The laser fixing plate is adjustablely embedded in the assembly cavity in the B direction and the C direction;

The laser is embedded in the laser fixing plate and is used to emit laser light in the A direction;

The laser fixing plate locking member is used to adjust and lock the laser fixing plate.

In order to solve the above technical problem, another technical solution adopted in this application is to provide a sample analyzer, which includes the aforementioned laser assembly, and the laser assembly is used to emit laser light to cooperate with optical detection.

The beneficial effect of the present application is that, different from the state of the prior art, the analyzer and laser assembly provided in the present application have a compact structure, convenient adjustment, and are not prone to jams.

Description of the drawings

In order to more clearly describe the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed in the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative work, other drawings can be obtained based on these drawings, among which:

Figure 1 is a simplified schematic diagram of an optical path of an optical detection device provided by an embodiment of the present application;

2 is a schematic diagram of a three-dimensional structure of an optical detection device provided by an embodiment of the present application;

3 is a schematic diagram of partial components of the optical detection device shown in FIG. 2;

4 is a schematic diagram of partial components of the optical detection device shown in FIG. 3;

5 is a schematic diagram of partial components of the optical detection device shown in FIG. 4;

FIG. 6 is a partial assembly schematic diagram of the laser assembly shown in FIG. 3;

FIG. 7 is a three-dimensional schematic diagram of the laser assembly shown in FIG. 3;

FIG. 8 is a three-dimensional schematic diagram of the laser assembly shown in FIG. 3;

FIG. 9 is a schematic side view of the laser assembly shown in FIG. 3;

10 is a schematic cross-sectional view of the laser assembly shown in FIG. 7;

Fig. 11 is a partial assembly diagram of the laser assembly shown in Fig. 7;

Fig. 12 is a partial assembly diagram of the laser assembly shown in Fig. 7;

FIG. 13 is a partial assembly diagram of the laser assembly shown in FIG. 7;

FIG. 14 is a partial assembly diagram of the laser assembly shown in FIG. 7;

Fig. 15 is a schematic diagram of a fixing method of the laser assembly shown in Fig. 2;

16 is a schematic diagram of partial components of the optical detection device shown in FIG. 2;

Figure 17 is an exploded view of the fluorescence receiving assembly shown in Figure 16;

Figure 18 is an exploded view of the fluorescence receiving assembly shown in Figure 16;

Figure 19 is a partial cross-sectional view of the optical detection device shown in Figure 2;

20 is a schematic diagram of the components of the optical detection device shown in FIG. 2;

FIG. 21 is an exploded schematic diagram of the side-scattered light focusing lens adjustment assembly shown in FIG. 20;

Figure 22 is a perspective schematic view of the sheath flow cell assembly shown in Figure 16;

Fig. 23 is a cross-sectional view of the sheath flow cell assembly and the side-scattered light focusing lens adjusting assembly shown in Fig. 20.

Detailed ways

The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of this application.

It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, the directional indications are only used to explain that they are in a specific posture (as shown in the drawings). If the specific posture changes, the relative positional relationship, movement, etc. of the components below will also change the directional indication accordingly.

In addition, if there are descriptions related to "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only used for descriptive purposes, and cannot be understood as instructions or implications Its relative importance or implicitly indicates the number of technical features indicated. Therefore, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on what can be achieved by a person of ordinary skill in the art. When the combination of technical solutions is contradictory or cannot be achieved, it should be considered that this combination of technical solutions does not exist , Is not within the scope of protection required by this application.

As shown in FIGS. 1 and 2, the present application provides an optical detection device, which includes an optical substrate 100, a first optical adjustment member 111, a second optical adjustment member 112, a sheath flow cell assembly 200, and side scattering The light focusing lens 250, the laser assembly (or front light assembly) 300, the light source substrate 310, the forward scattered light receiving assembly 400, the side light splitting assembly 500, the side scattered light receiving assembly 600, and the fluorescence receiving assembly 700.

In this application, the extension direction of the optical axis of the laser assembly 300 (shown with reference to the dashed line in FIG. 10) is defined as the A direction, the direction perpendicular to the A direction on the horizontal plane is defined as the B direction, and the vertical plane is relative to the A direction. The vertical direction is defined as the C direction, which can be equivalent to the X/Y/Z direction of the spatial rectangular coordinate system. Of course, the three directions can also be arranged non-vertically.

The light source substrate 310 is slidably arranged on the optical substrate 100 in the B direction, the sheath flow cell assembly 200 is arranged on the optical substrate 100, and the laser assembly 300 is arranged on the light source substrate 310 and is located on the first side of the sheath flow cell assembly 200 By the way, the laser assembly 300 includes a laser 340, a collimator lens 341, a front light focusing lens 342, and a cylindrical lens 343, etc., which are used to provide laser light to the sheath flow cell assembly 200, and the forward scattered light receiving assembly 400 is provided in the sheath flow cell. The component 200 is far away from the laser component 300. The three components of the forward scattered light receiving component 400, the sheath flow cell component 200 and the laser component 300 are roughly arranged in a first linear direction. The forward scattered light receiving component 400 includes the forward The scattered light receiving plate 410, the forward scattered light receiving aperture 420, and at least one front dispersion adjusting member 430 are adjusted in the A direction, the B direction or the C direction to improve the light receiving accuracy and the detection accuracy. The front dispersion adjusting member is preferred Adjust in the B direction. Of course, it can also be adjusted in two dimensions. For example, adjust in the A direction or the C direction or both directions A and C at the same time. The forward scattered light receiving assembly 400 is used to receive after passing through the sheath flow cell assembly 200. For the forward laser, the side-scattered light focusing lens 250 is arranged on the second side of the sheath flow cell assembly 200, the second side and the first side may be perpendicular to each other, and the side-scattered light focusing lens 250 is used to receive the passing light For the side laser behind the sheath flow cell assembly 200, the side beam splitting assembly 500 is arranged on the side of the side scattered light focusing lens 250 away from the sheath flow cell assembly 200, the side beam splitting assembly 500, the side scattered light focusing lens 250 and the sheath The three elements of the flow cell assembly 200 are roughly arranged in the second linear direction, and the second linear direction and the aforementioned first linear direction may be perpendicular to each other. The lateral beam splitting assembly 500 includes a dichroic mirror 510 and a filter 520, The lateral light splitting assembly 500 has the functions of light splitting and filtering. The lateral light splitting assembly 500 is used to receive the side laser light passing through the side scattered light focusing lens 250. The side scattered light receiving assembly 600 is arranged on the first side of the side light splitting assembly 500. On the side, the side scattered light receiving assembly 600 includes a side receiving plate 610, a side scattered light receiving aperture 620, and at least one side dispersion adjusting member (not shown) to improve light collection in the A direction or the B direction or the C direction To improve the accuracy of detection, the side dispersion adjuster is preferably adjusted in the B direction. Of course, it can also be adjusted in two dimensions. For example, it can be adjusted in the A direction or the C direction or both A and C at the same time, and the side scattered light is received. The assembly 600 is used to receive the laser light reflected by the lateral beam splitting assembly 500. The fluorescence receiving assembly 700 is arranged on the second side of the lateral beam splitting assembly 500. The fluorescence receiving assembly 700 includes a fluorescence receiving plate 740 and a fluorescence receiving aperture 741. The fluorescence receiving assembly 700 is used to receive the laser light transmitted by the lateral beam splitting assembly 500. The fluorescence receiving assembly 700 includes an adjustment mechanism 752 and a fluorescence receiver 742 arranged side by side or stacked. As shown in FIG. 2, the adjustment mechanism 752 and the fluorescence receiver 742 are arranged side by side When installed, the fluorescent receiver assembly 700 is vertically placed in a long strip, and the adjustment mechanism 752 extends to the side where the laser assembly 300 is located. The limited space is fully utilized to make the overall structure of the product compact. If the adjustment mechanism 752 and the fluorescent receiver 742 are stacked In this way, the adjustment mechanism 752 is arranged on the side of the fluorescent receiver 742 away from the lateral beam splitting assembly 500, the overall length of the fluorescent receiving assembly 700 will be shorter, but the thickness will increase.

Among them, the point light source emitted by the laser 340 passes through the collimator lens 341, the front light focusing lens 342, and the cylindrical lens 343 to form the elliptical shape needed to reach the sheath flow cell assembly 200 (the long axis is about 100-200um, the short The axis is about 10-30um) light spot, which forms the light source/beam of flow cytometry; the light irradiates the particles (blood cells, etc.) in the sheath flow cell 201 of the sheath flow cell assembly 200 to generate scattered light in various directions/angles, And the excited fluorescence generated by irradiating the fluorescent dyed particles; the forward scattered light receiving aperture 420 is used to selectively receive the scattered light signal within a certain angle range, generally a small angle range, such as 1-9°, the signal It characterizes the size information of the particle volume; the side scattered light at a certain angle to the beam, generally 90°, needs to collect a large angle (range of scattered light, generally the large angle is 90° with the laser 340) due to side scattering Axial center and an angle within the range of ±30°. Therefore, after collecting and focusing by the side-scattered light focusing mirror 250, it first reaches the dichroic mirror 510, which has the function of reflection and projection to select a certain range of wavelengths, and collects The side-scattered light (reflecting the complexity of cell membrane, cytoplasm, nuclear membrane and the size of the cell nucleus) is reflected to the side-scattered light-receiving component 600, and at the same time the excited fluorescence is selectively transmitted, and reaches the fluorescence-receiving component 700.

As shown in FIGS. 1 to 16, the laser assembly 300 includes a light source substrate 310, a light source base 320, a laser fixing plate 330, a laser 340, a locking plate 350, a locking plate fixing member 351, a first light source adjusting member 331, and a second light source The adjusting part 332, the laser pressing plate 344, the laser pressing plate locking part 345, the laser fixing plate locking part 333, the elastic pushing part 334, and the translation matching part 336.

The light source substrate 310 is a machined part with good flatness. The light source base 320 is provided with a through assembly cavity 322. The assembly cavity 322 can be in the shape of a stepped cavity (including a circular cavity 340 for assembling a laser and a rectangular cavity for assembling a laser fixing plate 330). Cavity) to facilitate the limited assembly of internal components (such as laser fixing plate 330 and collimator lens 341). The light source base 320 also has a relief groove 353 at the matching end corner of the assembly cavity 322 and the laser pressing plate 344 to facilitate the laser pressing plate 344 Make adjustments.

The light source base 320 is slidably arranged on the light source substrate 310 in the A direction, the laser fixing plate 330 is adjustable in the A direction, the B direction, and the C direction. The laser 340 is embedded in the assembly cavity 322. In the fixing plate 330, the laser 340 is used to emit laser light in the A direction. In order to make the optical axis of the laser 340 in an ideal state (for example, coaxial with the axis of the assembly cavity 322), the laser fixing plate 330 is set in the A direction in this application. , B direction, C direction three directions to adjust.

The laser 340 can be pressed into the laser fixing plate 330 through the laser pressing plate 344 and locked by the laser pressing plate locking part 345. The laser pressing plate locking part 345 can be a screw, a fastener or an adhesive part, etc. The laser fixing plate 330 is provided with There is a stepped cavity. The laser pressure plate 344 includes a sleeve part and a flange part. The sleeve part limits the laser 340 to the stepped cavity of the laser fixing plate 330. The laser pressure plate locking member 345 locks the flange part on the laser The fixing plate 330 is used to fix the laser 340.

The locking plate 350 presses the laser fixing plate 330 into the light source base 320 through the locking plate fixing member 351. The locking plate fixing member 351 can be a screw, a fastener or an adhesive member, etc. The laser fixing plate locking member 333 penetrates the locking plate 350 to adjust and lock the laser fixing plate 330. The laser fixing plate locking pieces 333 can be screws, etc. The number of the laser fixing plate locking pieces 333 can be one, two or more. When the number of the laser fixing plate locking pieces 333 is two, they can be arranged diagonally and diagonally. By rotating any one of the two laser fixing plate locking members 333 clockwise or counterclockwise, the position of the laser fixing plate 330 can be adjusted, for example, the position of the laser fixing plate 330 can be adjusted to a vertical state.

The first light source adjusting member 331 extends into the light source base 320 through the adjusting hole 329 (see FIG. 6) to adjust the laser fixing plate 330 in the B direction, and the second light source adjusting member 332 extends into the light source base 320 through the adjusting hole 329 to The laser fixing plate 330 is adjusted in the C direction. The first light source adjusting member 331 and the second light source adjusting member 332 can be screws, thread pairs, etc., because the assembly accuracy of the laser fixing plate 330 is very high, in some embodiments, the first The light source adjusting piece 331 and the second light source adjusting piece 332 can be removed after the adjustment is completed to avoid wrong adjustment by non-professionals. Of course, the first light source adjusting piece 331 and the second light source adjusting piece 332 can also be left in the light source base 320 is convenient for professional debugging personnel to adjust.

As shown in FIG. 10, the light source base 320 is also provided with a convex ring portion 323 corresponding to the assembling cavity. The convex ring portion 323 can be positioned and sleeved with the lens barrel portion 324 shown in FIGS. Optical lenses such as the aforementioned front light focusing lens 342 and cylindrical lens 343 are provided. The lens barrel portion 324 and the light source base 320 can be integrally formed or split-shaped, and the precision when integrally formed is relatively high, and the split-shaped configuration is convenient to manufacture.

The first optical adjustment member 111 can be installed through the light source substrate 310 or other additional boards and connected to the light source base 320. The light source base 320 has a corresponding connection hole 113 for adjusting the light source base 320 relative to the light source substrate in the A direction. 310 sliding, the second optical adjustment member 112 is installed through the optical substrate 110 or other additional boards and connected to the light source substrate 310 for adjusting the sliding of the light source substrate 310 relative to the optical substrate 100 in the B direction, the first optical adjustment member 111 And the second optical adjustment member 112 may be an adjustment mechanism such as a thread pair, a slider assembly, a motor assembly, a gear assembly, etc., preferably a thread pair.

The adjustment requirements can be better met by the four adjusting parts and the laser fixing plate locking part 333. Among them, the first light source adjusting part 331 and the second light source adjusting part 332 can be used to adjust up and down, and the first optical adjusting part 111 and The second optical adjustment member 112 can be relatively adjusted back and forth, left and right, and the laser fixing plate locking member 333 can adjust the overall inclination of the surface of the laser fixing plate 330.

The elastic pushing member 334 is embedded and assembled on the light source base 320 through the embedded hole 328 (see FIG. 6) and elastically abuts against the surface of the laser fixing plate 330 away from the first light source adjusting member 331 and the second light source adjusting member 332, The elastic pushing member 334 can be an elastic ball plunger, a spring or elastic silicone. The translational fitting part 336 is embedded and assembled in the light source base 320 and abuts against the surface of the laser fixing plate 330 away from the locking plate 350. The first light source is adjusted The member 331 and the second light source adjusting member 332 form an adjusting mechanism with a mutual pushing force with the elastic pushing member 334 during screw adjustment to ensure better adjustment controllability. The laser fixing plate locking member 333 forms an adjustment mechanism with a mutual pushing force with the translation matching portion 336 during screw adjustment to ensure better adjustment control. Of course, the elastic pushing member 334 only plays a passive cooperating role, and will not push the position of the laser fixing plate locking member 333 to change due to the removal of the first light source adjusting member 331 and the second light source adjusting member 332.

The light source base 320 or the light source substrate 310 is provided with a protruding sliding fitting portion 321 (see FIG. 6) to reduce friction when the slidability is adjusted in the A direction, and the light source substrate 310 or the optical substrate 100 is provided with a protruding type The sliding fitting portion 311 (see FIG. 5) is provided to reduce friction when adjusting the slidability in the B direction. The sliding fitting portion (311, 321) may be an integral part of the optical substrate 100 or the light source substrate 310 and the light source base 320 The protruding part of the structure, or the sliding mating part (311, 321) may be elastic ball plungers or balls embedded on the optical substrate 100, the light source substrate 310 or the light source base 320 (see Fig. 8 and Fig. 9), By reducing the contact area to reduce the friction, the sliding adjustment is smoother.

As shown in FIGS. 16-18, the fluorescence receiving assembly 700 includes a fixed seat 730, a first moving substrate 710, a second moving substrate 720, a fluorescence receiving plate 740, a fluorescence receiver 742, a first fluorescence adjustment member 701, and a second fluorescence The adjusting member 702, the first locking member 716, and the second locking member 726. The first locking member 716 and the second locking member 726 may be screws. The fluorescent receiving assembly 700 includes an adjusting mechanism 752 and a fluorescent receiver 742 arranged side by side or stacked. As shown in FIG. 2, when the adjusting mechanism 752 and the fluorescent receiver 742 are arranged side by side, the fluorescent receiving assembly 700 is placed vertically as a whole. The adjustment mechanism 752 extends to the side where the laser assembly 300 is located, making full use of the limited space to make the overall structure of the product compact. If the adjustment mechanism 752 and the fluorescent receiver 742 are arranged in a layered manner, that is, the adjustment mechanism 752 is arranged on the fluorescent receiver. 742 is far from the side of the lateral beam splitting component 500, the overall length of the fluorescent receiving component 700 will be shorter, but the thickness will be increased.

The first movable substrate 710 is slidably adjustable in the C direction and is arranged on the fixed base 730, the second movable substrate 720 is slidably adjustable in the A direction and arranged on the first movable substrate 710, and the fluorescent receiver 742 is fixed on the second Move on the substrate 720. Of course, in some embodiments, the second movable substrate 720 may not be provided, and the fluorescent receiving plate 740 may be slidably arranged on the first movable substrate 710.

The fixing base 730 may include a main body plate 731 and a folding plate 732 arranged on the side of the main body plate 731. The folding plate 732 is used for setting the first fluorescence adjusting member 701 to adjust the first movable substrate 710 in the C direction. The first fluorescence adjusting member 701 can be an adjustment mechanism such as a thread pair, a slider assembly, a motor assembly, a gear assembly, etc.

The main body plate 731 is provided with a first guide post 734, the first moving base plate 710 is provided with a C guide groove 714 matching the first guide post 734, and the main body plate 731 is provided with a first locking hole 735. The base plate 710 is provided with a first locking groove 715 that matches the first locking hole 735. The first locking member 716 passes through the first locking groove 715 to cooperate with the first locking hole 735. On the first moving base plate 710 A second guide post 714 is provided, and an A guide groove 724 matching the second guide post 714 is provided on the second movable substrate 720.

The first moving base plate 710 is provided with a second locking hole 715, the second moving base plate 720 is provided with a second locking groove 725 matching the second locking hole 715, and the second locking member 726 passes through the second lock The tightening groove 725 is matched with the second locking hole 715. The first moving base plate 710 includes a first plate 711 and a second plate 712 and a third plate 713 that are bent oppositely from the edge of the first plate 711. A plate body 711 is attached to the main body plate 731, the second plate body 712 is parallel to the folded plate 732, and the third plate body 713 is used for setting the second fluorescence adjusting member 702 to adjust the second moving substrate 720 in the A direction.

The fluorescent receiving board 740 is fixed to the second mobile substrate 720, the fluorescent receiver 742 is fixed on the fluorescent receiving board 740, and the second mobile substrate 720 is provided with a through slot 721 aligned with the fluorescent receiver 742. The fluorescent receiver 742 can be embedded Assembled in the through groove 721, the fluorescence receiving assembly 700 further includes a fluorescent diaphragm 741, the fluorescent diaphragm 741 is fixed on the second movable substrate 720 and covers the through groove 721, the fluorescent diaphragm 741 is arranged to be aligned with the fluorescent receiver 742的光孔743.

As shown in FIG. 19, the optical detection device further includes an optical bottom case 91, an optical cover matched with the optical bottom case 91, a lower shock-absorbing member 93, an upper shock-absorbing member 94, and a first connecting member 95. The optical bottom case 91 is provided with There is a second connecting piece 96. The second connecting piece 96 can be a snap or threaded post formed integrally or assembled on the optical bottom case 91. The second connecting piece 96 has a snap position or an internal thread, and the lower shock absorber 93 It is sleeved on the outer periphery of the second connecting member 96. The optical substrate 100 is provided with a lower step hole 104 and an upper step hole 105. The optical substrate 100 is sleeved on the second connecting member 96 and pressed on the lower shock-absorbing member 93 through the lower step hole 104 The outer peripheral wall of the upper shock-absorbing member 94 is stepped, the upper shock-absorbing member 94 is step-fitted with the upper step hole 105 and is spaced between the second connecting member 96 and the optical substrate 100, and the bottom surface of the upper shock-absorbing member 94 and the lower shock-absorbing member 94 The top end of the shock element 93 can be arranged at intervals, and the top end of the upper shock absorber 94 is higher than the top end of the second connecting element 96. The first connecting element 95 and the second connecting element 96 are snap-connected or screw-connected and pressed into the upper shock absorber. The top surface of the vibration member 94 has a better anti-vibration support effect. The upper shock-absorbing member 94 or the lower shock-absorbing member 93 may be components with good elastic properties such as a silicone tube or a spring.

The optical detection device provided by the present application has a compact structure, convenient adjustment, and is not prone to jamming. The laser assembly 300 can be adjusted in multiple dimensions through four adjustment parts and the laser fixing plate locking part 333, and the operation is flexible and convenient. In addition, by setting a sliding fitting part 321 can reduce the friction during adjustment, can adjust smoothly, and avoid jamming. The present application also provides a sample analyzer, the sample analyzer includes the aforementioned optical detection device, and the sample analyzer may specifically be a blood cell analyzer, a flow cytometer, a coagulation analyzer, an immune analyzer, and the like.

As shown in FIGS. 1 to 14, the present application also provides a laser assembly 300, which includes an optical substrate 100, a first optical adjustment member 111, a second optical adjustment member 112, a light source substrate 310, and a light source base 320 , Laser fixing plate 330, laser 340, locking plate 350, first light source adjusting member 331, second light source adjusting member 332, laser fixing plate locking member 333, elastic pushing member 334, translation matching portion 336, collimating lens 341, The first oblique pressing member 360 and the second oblique pressing member 370.

Among them, the light source substrate 310 is a machined part with good flatness. The light source base 320 is slidably arranged on the light source substrate 310 in the A direction, and the light source substrate 320 is slidably arranged on the optical substrate 310 in the B direction. Above, the first optical adjustment member 111 is installed through the light source substrate 310 or other additional boards and connected to the light source base 320 for adjusting the sliding of the light source base 320 relative to the light source substrate 310 in the A direction. The light source base 320 has The first optical adjustment part 111 is matched with the connecting hole 113 (see FIG. 7). The second optical adjustment part 112 is installed through the optical substrate 110 or other additional boards and connected to the light source substrate 310. The light source substrate 310 has a connection with the second optical adjustment part. The connecting hole 114 corresponding to 112 (see FIG. 5) is used to adjust the sliding of the light source substrate 310 relative to the optical substrate 100 in the B direction. The first optical adjustment member 111 and the second optical adjustment member 112 may be adjustment mechanisms such as a thread pair, a slider assembly, a motor assembly, and a gear assembly.

The light source base 320 is provided with a through assembly cavity 322, which can be in the shape of a stepped cavity (including a circular cavity 340 for assembling a laser and a rectangular cavity for assembling a laser fixing plate 330) to facilitate internal components (such as laser fixing plate 330, collimating lens) 341) Perform limit assembly.

The laser fixing plate 330 is adjustably embedded in the assembly cavity 322 in the A direction, the B direction, and the C direction. The laser 340 is embedded in the laser fixing plate 330 for emitting laser light in the A direction. The axis is in an ideal state (for example, coaxial with the axis of the assembly cavity 322), and the laser fixing plate 330 can be adjusted in the three directions of the A direction, the B direction, and the C direction in the present application.

The laser collimating lens 341 is embedded in the assembly cavity 322 and located on the front side of the laser 340. The locking plate 350 cooperates with the light source base 320 to press the laser fixing plate 330 into the light source base 320. The laser fixing plate locking piece 333 penetrates through the locking plate 350 to adjust the laser fixing plate 330 and lock the posture of the laser fixing plate 330 after the adjustment. The laser fixing plate locking pieces 333 can be screws, etc. The number of the laser fixing plate locking pieces 333 can be one, two or more. When the number of the laser fixing plate locking pieces 333 is two, they can be arranged diagonally and diagonally. By rotating any one of the two laser fixing plate locking members 333 clockwise or counterclockwise, the position of the laser fixing plate 330 can be adjusted, for example, the position of the laser fixing plate 330 can be adjusted to a vertical state.

The first light source adjusting member 331 extends into the light source base 320 through the adjusting hole 329 (see FIG. 6) to adjust the laser fixing plate 330 in the B direction, and the second light source adjusting member 332 extends into the light source through the adjusting hole 329 (see FIG. 6) The base 320 is used to adjust the laser fixing plate 330 in the C direction. The first light source adjusting member 331 and the second light source adjusting member 332 may be screws, thread pairs, etc., because the assembly accuracy of the laser fixing plate 330 is very high, in some embodiments, the first light source adjusting member 331 and the second light source adjusting After the adjustment is completed, the piece 332 can be taken away to avoid wrong adjustment by non-professionals. Of course, the first light source adjusting piece 331 and the second light source adjusting piece 332 can also be left on the light source base 320 to facilitate adjustment by professional debugging personnel. .

As shown in FIG. 10, the light source base 320 is also provided with a convex ring portion 323 corresponding to the assembling cavity. The convex ring portion 323 can be positioned and sleeved with the lens barrel portion 324 shown in FIGS. 8 and 9, and the lens barrel portion 324 can be used for Optical lenses such as the aforementioned front light focusing lens 342 and cylindrical lens 343 are provided.

The elastic pushing member 334 is embedded and assembled on the light source base 320 and elastically abuts against the surface of the laser fixing plate 330 away from the first light source adjusting member 331 and the second light source adjusting member 332. The elastic pushing member 334 may be an elastic ball head Plunger, spring or elastic silicone.

The translational matching portion 336 is embedded and assembled in the light source base 320. The translational matching portion 336 abuts on the surface of the laser fixing plate 330 away from the locking plate 350. The translational matching portion 336 can be an elastic ball plunger, a ball, or an integral structure. Protruding parts to reduce friction during sliding adjustment. The first light source adjusting member 331 and the second light source adjusting member 332 form a mutual pushing force with the elastic pushing member 334 during screw adjustment to ensure better adjustment controllability. The laser fixing plate locking member 333 forms a mutual pushing force with the translation matching portion 336 during screw adjustment to ensure better adjustment control. Of course, the elastic pushing member 334 only plays a passive cooperating role, and will not push the position of the laser fixing plate locking member 333 to change due to the removal of the first light source adjusting member 331 and the second light source adjusting member 332.

The first oblique pressing member 360 is fixed to the light source substrate 310, the light source base 320 is provided with a first crimping portion 361 matching the first oblique pressing member 360, and the first oblique pressing member 360 is an elastic pressing piece, pressing block or elastic The ball plunger and the first oblique pressing member 360 can be fixed by the light source substrate 310, for example, as shown in FIG. 3. When the first oblique pressing member 360 is an elastic ball plunger, as shown in FIG. 15, the first oblique pressing member 360 not only plays a role of elastically pressing the light source base 320 in an oblique direction, but at the same time, the crimping part is a spherical surface. Contact, the friction resistance is small, and the adjustment is relatively smooth. The bottom surface and/or the side surface of the light source substrate 310 are provided with elastic ball plungers, balls, or protruding sliding fitting parts 321 to reduce friction when adjusting the slidability.

The second oblique pressing member 370 is fixed to the optical substrate 100, the light source substrate 310 is provided with a second crimping portion 371 that matches the second oblique pressing member 370, and the second oblique pressing member 370 is an elastic pressing piece or an elastic ball plunger The bottom surface and/or side surface of the light source substrate 310 are provided with elastic ball plungers, balls, or protruding sliding fitting parts to reduce friction when adjusting the sliding properties.

The laser assembly 300 may also include a heating element (not shown in the figure), a temperature sensor 382, and a temperature switch (not shown in the figure). The light source base 320 is provided with an assembly groove 381 or assembly holes (383, 384). The sensor 382 and the temperature switch are installed in the assembling groove 381 or the assembling holes (383, 384). When the temperature sensor 382 is installed in the assembling groove 381, it can be covered by the cover plate 385. The heating element can ensure the collimating lens 341 , The front light focusing mirror 342 and the cylindrical mirror 343 work at a constant temperature to ensure the consistency of light output. The outer circumference of the light source base 320 can be further wrapped with thermal insulation cotton to improve the thermal insulation effect.

The laser assembly 300 provided in the present application has a compact structure, convenient adjustment, and is not prone to jamming. The laser assembly 300 can be adjusted in multiple dimensions through four adjustment parts and the laser fixing plate locking part 333, and the operation is flexible and convenient. In addition, the sliding fitting part 321 is provided. It can reduce the friction during adjustment, can adjust smoothly, and avoid jamming. The present application also provides a sample analyzer, which includes the aforementioned laser assembly 300. The sample analyzer may specifically be a blood cell analyzer, a flow cytometer, a coagulation analyzer, an immune analyzer, and the like.

As shown in FIGS. 1 to 16, the present application also provides an optical detection device. The optical detection device includes an optical substrate 100, a light source substrate 310, a laser assembly 300, a first oblique pressing member 360, and a second oblique pressing member 370.

As shown in Figures 4 and 5, the light source substrate 310 is slidably adjustable in the B direction and is set on the optical substrate 100, wherein the light source substrate 310 or the optical substrate 100 is provided with a protruding sliding mating portion 311 to perform Reduce friction during sliding adjustment. The light source substrate 310 is provided with a guide adjustment groove 115. After the light source substrate 310 is slidably adjusted in the B direction, the adjustment position can be locked by a screw 116 passing through the guide adjustment groove 115.

As shown in Figures 3 and 6, the laser assembly 300 is slidably adjustable in the A direction on the light source substrate 310, the laser assembly 300 or the light source substrate 310 is provided with a sliding fitting portion 321, and the laser assembly 300 is provided with a guide adjustment groove 354 After the laser assembly 300 is slidably adjusted in the A direction, the adjustment position can be locked by a screw 355 (see FIG. 3) passing through the guide adjustment slot 354.

The sliding fitting portion 311 is a protruding portion of an integral structure on the optical substrate 100 or the light source substrate 310, or the sliding fitting portion 311 is an elastic ball plunger or a ball embedded on the optical substrate 100 or the light source substrate 310.

The light source substrate 310 is provided with a first sliding adjustment area 372. The laser assembly 300 is slidably arranged in the first sliding adjustment area 372. The first sliding adjustment area 372 is composed of a first bottom wall 373 and a first guide side wall 374. The laser assembly 300 abuts against the first guide side wall 374, the first optical adjustment member 111 is installed through the light source substrate 310 and connected to the light source base 320 for adjusting the sliding of the light source base 320 relative to the light source substrate 310 in the A direction. A sliding adjustment area 372 may be an area recessed on the surface of the light source substrate 310, or the light source substrate 310 may be provided with ribs to provide the first guiding side wall 374.

As shown in FIG. 3 or FIG. 15, the first oblique pressing member 360 is fixed to the light source substrate 310, and is used for elastically pressing the laser assembly 300 so that a surface of the laser assembly 300 abuts against the first guide side wall 374. An oblique pressing member 360 is an elastic pressing piece or an elastic ball plunger, and the laser assembly 300 is provided with a first crimping portion 361 abutting against the first oblique pressing member 360 (see FIG. 7).

As shown in FIG. 16, the optical substrate 100 is provided with a second sliding adjustment area 101, and the light source substrate 310 is slidably provided in the second sliding adjustment area 101. The second sliding adjustment area 101 is formed by the second bottom wall 102 and the second guide side. The light source substrate 310 is abutted against the second guiding side wall 103. The optical detection device further includes a second optical adjustment member 112. The first optical adjustment member 112 is used to adjust the light source substrate 310 relative to the optical substrate 100 in the B direction. Sliding, the second sliding adjustment area 101 may be an area recessed on the surface of the optical substrate 100, or the optical substrate 100 may be provided with convex ribs to provide the second guiding side wall 102.

The second oblique pressing member 370 is fixed to the optical substrate 100 or other additional plates, and is used to buckle the light source substrate 310 obliquely and elastically so that a surface of the light source substrate 310 abuts against the second guiding side wall 102, and the second oblique pressing The member 370 is an elastic pressing piece or an elastic ball plunger, and the light source substrate 310 is provided with a second crimping portion 371 that abuts against the second oblique pressing member 370,

The optical detection device provided by the present application has a compact structure, convenient adjustment, low friction, and is not prone to jamming. The light source substrate 310 is slidably adjustable in the B direction and arranged on the optical substrate 100, and the light source substrate 310 or the optical substrate 100 is provided with The protruding sliding fitting portion 311 is used to reduce the frictional force when the sliding property is adjusted. The present application also provides a sample analyzer, the sample analyzer includes the aforementioned optical detection device, and the sample analyzer may specifically be a blood cell analyzer, a flow cytometer, a coagulation analyzer, an immune analyzer, and the like.

As shown in FIGS. 16-18, the present application also provides a fluorescence receiving assembly 700, which includes a fixing base 730, a first moving substrate 710, a second moving substrate 720, a fluorescence receiving plate 740, and a fluorescence receiver 742 , The first fluorescence adjusting member 701, the second fluorescence adjusting member 702, the first locking member 716, and the second locking member 726. The fluorescent receiving assembly 700 includes an adjusting mechanism 752 and a fluorescent receiver 742 arranged side by side or stacked. As shown in FIG. 2, when the adjusting mechanism 752 and the fluorescent receiver 742 are arranged side by side, the fluorescent receiving assembly 700 is placed vertically as a whole. The adjustment mechanism 752 extends to the side where the laser assembly 300 is located, making full use of the limited space to make the overall structure of the product compact. If the adjustment mechanism 752 and the fluorescent receiver 742 are arranged in a layered manner, that is, the adjustment mechanism 752 is arranged on the fluorescent receiver. 742 is far from the side of the lateral beam splitting component 500, the overall length of the fluorescent receiving component 700 will be shorter, but the thickness will be increased.

The first moving substrate 710 is slidably adjustable in the C direction and is arranged on the fixed base 730, the second moving substrate 720 is slidably adjustable in the A direction and arranged on the first moving substrate 710; the fluorescent receiver 742 is fixed on the second On the moving base plate 720, the fixing base 730 includes a main body plate 731 and a folding plate 732 provided on the side of the main plate 731. The folding plate 732 is used for setting the first fluorescence adjusting member 701 to adjust the first moving base plate 710 in the C direction.

The main body plate 731 is provided with a first guide post 734, the first moving base plate 710 is provided with a C guide groove 714 matching the first guide post 734, and the main body plate 731 is provided with a first locking hole 735. The base plate 710 is provided with a first locking groove 715 matching the first locking hole 735, and the first locking member 716 passes through the first locking groove 715 to fit with the first locking hole 735.

The first moving base plate 710 is provided with a second guide post 714, the second moving base plate 720 is provided with an A guide groove 724 matching the second guide post 714, and the first moving base plate 710 is provided with a second locking hole 715 , The second moving base plate 720 is provided with a second locking groove 725 matching the second locking hole 715, and the second locking member 726 passes through the second locking groove 725 to cooperate with the second locking hole 715. The mobile substrate 710 includes a first plate body 711, a second plate body 712 and a third plate body 713 that are bent in opposite directions from the edge of the first plate body 711. The first plate body 711 is attached to the main body plate 731, and the second plate body 711 is attached to the main body plate 731. The plate body 712 is parallel to the folded plate 732, and the third plate body 713 is used for setting the second fluorescence adjusting member 702 to adjust the second moving substrate 720 in the A direction.

The fluorescent receiving board 740 is fixed to the second mobile substrate 720, the fluorescent receiver 742 is fixed on the fluorescent receiving board 740, and the second mobile substrate 720 is provided with a through slot 721 aligned with the fluorescent receiver 742. The fluorescent receiver 742 can be embedded It is installed in the through groove 721 1, the fluorescent diaphragm 741 is fixed on the second movable substrate 720 and covers the through groove 721, and the fluorescent diaphragm 741 is provided with a light hole 743 that is aligned with the fluorescent receiver 742.

The fluorescence receiving assembly 700 provided in the present application has a compact structure, convenient adjustment, and is not prone to jamming. The adjustment mechanism 752 extends to the side where the laser assembly 300 is located, making full use of the limited space to make the overall structure of the product compact. The present application also provides a sample analyzer, which includes the aforementioned fluorescence receiving assembly 700, and the sample analyzer may specifically be a blood cell analyzer, a flow cytometer, a coagulation analyzer, an immune analyzer, and the like.

As shown in Figures 20 to 23, the present application also provides a side-scattered light focusing lens adjustment assembly. The side-scattering light focusing lens adjustment assembly includes a mounting seat 210, a support plate 220, an adjustment piece 230, a linkage block 240, and a guide The column 248, the locking member 252, the sheath flow cell assembly locking member 260, and the optical substrate 100.

The mounting seat 210 is provided with a side wall 211 and a top wall 212. The side wall 211 and the top wall 212 enclose an inner cavity 213. The top wall 212 is provided with an opening 214 communicating with the inner cavity 213 (see FIG. 21). The inner cavity 213 is used for The sheath flow cell assembly 200 is inserted and assembled and extends out of the opening 214 so that the sheath flow cell 201 of the sheath flow cell assembly 200 is disposed above the top wall 212. The sheath flow cell assembly 200 can be rotated and adjusted relative to the mounting seat 210 and fixed.

The linkage block 240 is adjustably arranged on the top wall 212, and the linkage block 240 is used to provide a side-scattered light focusing mirror 250 corresponding to the sheath flow cell 201.

The adjusting member 230 is connected with the linkage block 240 and is used to adjust the position of the linkage block 240 on the mounting seat 210 and thereby adjust the distance between the side scattered light focusing mirror 250 and the sheath flow cell 201. The adjusting member 230 may be an adjusting mechanism such as a screw, a thread pair, a sliding block assembly, a motor assembly, a gear assembly, and the like.

Specifically, the linkage block 240 includes a first linkage block 241, a second linkage block 242 that are connected, and a side of the first linkage block 241 away from the second linkage block 242 may be connected with a third linkage block 243, the first linkage block 241 A connecting hole 247 may be provided to connect with the adjusting member 230, or the third link block 243 may be provided with a connecting hole 247, the first link block 241 is slidably arranged on the top wall 212, and the second link block 242 is used to set side scattering Light focusing lens 250.

The second linkage block 242 has an extension portion 244 that is offset from the first linkage block 241, and the side-scattered light focusing lens 250 is disposed on the extension portion 244. In an embodiment, the second linkage block 242 may be L-shaped, and the side-scattered light focusing lens 250 is provided at the end of the L-shaped second linkage block 242.

The first linkage block 241 has a guide groove 246 corresponding to the guide post 248, and the top wall 212 has an assembly hole 249 corresponding to the guide post 248 for assembling the guide post 248. The first linkage block 241 is provided with a locking groove 245 corresponding to the locking member, and the top wall 212 is provided with a locking hole 253 corresponding to the locking member 252. The top wall 212 is provided with a through hole 261 corresponding to the sheath flow cell assembly locking member 260. The sheath flow cell assembly locking member 260 is used to pass through the through hole 261 to be connected to the sheath flow cell assembly 200. Correspondingly, the sheath flow cell assembly 200 A connecting flange 210 is provided, and a connecting screw hole 211 is provided on the connecting flange 210.

The supporting plate 220 is arranged on the side of the mounting seat 210, and the supporting plate 220 is used to support the adjusting member 230. The mounting seat 210 is fixed on the optical base plate 100, and the sheath flow cell assembly 200 penetrates the optical base plate 100.

The side-scattered light focusing lens adjustment assembly provided in the present application can avoid that when the conventional sheath flow cell assembly 200 is installed from top to bottom, the hand will touch the surface of the sheath flow cell 201 and cause pollution of the optical surface.

The application also provides a sample analyzer, which includes the aforementioned side-scattered light focusing lens adjustment component.

In the above embodiments, when adjusting, the sheath flow seat is used as a reference to adjust the light beam and/or focus of the light source or the receiving assembly to ensure that the beam can be aligned with the center of the sample particle flow in the sheath flow cell 201, and a suitable light spot is obtained. size. Therefore, the multi-component and multi-dimensional adjustment in this application not only fully guarantees the compactness and utilization of space, but also improves the accuracy of optical detection. It is of great significance to the detection of sample analyzers. The adjustment effects of each component are described in detail as follows:

The laser assembly has four-dimensional adjustment, that is, the optical substrate 100, the light source substrate 310, the first optical adjustment member 111, and the second optical adjustment member 112 work together to adjust the spot size in the A direction and the beam position in the B and C directions. , The ultimate goal is to ensure that the beam is aligned with the center of the sample particle flow in the sheath flow cell 201.

The sheath flow cell assembly 200 has two-dimensional adjustments, one is rotation adjustment, and the other is adjustment of the distance between the side-scattered light focusing lens 250, which is also used to calibrate the position and focus of the beam; in addition, due to the use of the fluorescence receiving assembly 700 Therefore, the sheath flow cell assembly 200 is only adjusted in the B direction, and the A direction is fixed. This is to prevent the movement in the A direction from affecting the optical detection of the forward scattered light receiving assembly 400. In addition, it is necessary to save the structure of the detection assembly and save space. More compact.

The forward scattered light receiving component 400 adopts one-dimensional adjustment, that is, the adjustment in the B direction, which is mainly to align the light beam with the light hole and improve the accuracy of light path detection; of course, it can also be adjusted in two dimensions, and by combining the adjustment mechanism and the receiving part The side-by-side arrangement makes the space more compact.

The fluorescence receiving assembly 700 adopts two-dimensional adjustment, and both the A-direction and B-direction adjustments make the light beam align with the diaphragm.

The side-scattered light receiving component 600 adopts one-dimensional adjustment, or two-dimensional adjustment, but it is preferably one-dimensional, and one-dimensional is also for aligning the receiving light hole with the light beam.

The above are only implementations of this application, and do not limit the scope of this application. Any equivalent structure or equivalent process transformation made using the content of the description and drawings of this application, or directly or indirectly applied to other related technical fields, The same reasoning is included in the scope of patent protection of this application.

Claims

A laser assembly including:

Light source base with assembly cavity;

The laser fixing plate is adjustablely embedded in the assembly cavity in the B direction and the C direction;

The laser is embedded in the laser fixing plate and is used to emit laser light in the A direction;

The laser fixing plate locking member is used to adjust and lock the laser fixing plate.
The laser assembly according to claim 1, wherein the laser assembly further comprises:

The locking plate cooperates with the light source base to press the laser fixing plate into the light source base, and the laser fixing plate locking member penetrates the locking plate to adjust and adjust the laser fixing plate. locking.
The laser assembly according to claim 1, wherein the laser assembly further comprises:

The first light source adjusting member penetrates the light source base to adjust the laser fixing plate in the B direction;

The second light source adjusting member penetrates the light source base to adjust the laser fixing plate in the C direction.
The laser assembly according to claim 3, wherein the laser assembly further comprises an elastic pusher, the elastic pusher elastically abuts on the laser fixing plate away from the first light source adjusting member and the second 2. The surface of the light source adjusting member.
4. The laser assembly according to claim 4, wherein the elastic pushing member is an elastic ball plunger, a spring, or elastic silicone.
The laser assembly according to claim 1, wherein the laser assembly further comprises a translational matching part embedded in the light source base and abutting against the laser fixing plate away from the locking plate s surface.
7. The laser assembly according to claim 6, wherein the translational mating portion is an elastic ball plunger, a ball, or a protruding portion of an integral structure to reduce friction when the laser fixing plate is slidably adjusted.
The laser assembly according to claim 1, wherein the laser assembly further comprises a laser collimating lens, and the laser collimating lens is embedded in the assembly cavity and located on the front side of the laser.
The laser assembly according to claim 1, wherein the laser assembly further comprises a heating element, a temperature sensor and a temperature switch, the light source base is provided with an assembly groove or assembly hole, the heating element, the temperature sensor and The temperature switch is installed in the assembling groove.
The laser assembly according to claim 1, wherein the laser assembly further comprises:

The light source substrate, the light source base is slidably adjustable in the A direction and is arranged on the light source substrate.
The laser assembly of claim 10, wherein the laser assembly further comprises:

An optical substrate, wherein the light source substrate is slidably adjustable in the B direction and arranged on the optical substrate.
The laser assembly of claim 10, wherein the laser assembly further comprises:

The first optical adjusting member is connected with the laser assembly to adjust the sliding of the light source base relative to the light source substrate in the A direction.
The laser assembly of claim 11, wherein the laser assembly further comprises:

The second optical adjusting member is connected with the light source substrate to adjust the sliding of the light source substrate relative to the optical substrate in the B direction.
The laser assembly of claim 10, wherein:

The laser assembly further includes a first oblique pressing member, the first oblique pressing member is fixed to the light source substrate, and the light source base is provided with a first crimping portion matching the first oblique pressing member, The first oblique pressing member is an elastic pressing piece or an elastic ball plunger.
The laser assembly according to claim 10, wherein the bottom surface and/or side surface of the light source base is provided with a sliding fitting part, and the sliding fitting part is an elastic ball plunger, a ball, or a protruding bump Part to reduce friction when adjusting sliding properties.
The laser assembly of claim 11, wherein:

The laser assembly further includes a second oblique pressing member, the second oblique pressing member is fixed to the optical substrate, the light source substrate is provided with a second crimping portion matching the second oblique pressing member, and The second oblique pressing member is an elastic pressing piece or an elastic ball plunger.
The laser assembly of claim 10, wherein:

The bottom surface and/or the side surface of the light source substrate are provided with sliding fitting parts, and the sliding fitting parts are elastic ball plungers, balls or protruding bumps to reduce friction during sliding adjustment.
A sample analyzer, wherein the sample analyzer comprises the laser assembly according to claim 1, and the laser assembly is used to emit laser light to cooperate with optical detection.
The sample analyzer of claim 18, wherein the laser assembly further comprises:

The locking plate cooperates with the light source base to press the laser fixing plate into the light source base, and the laser fixing plate locking member penetrates the locking plate to adjust and adjust the laser fixing plate. locking;

The first light source adjusting member penetrates the light source base to adjust the laser fixing plate in the B direction;

The second light source adjusting member penetrates the light source base to adjust the laser fixing plate in the C direction.
The sample analyzer according to claim 18, wherein the laser component further comprises a fluorescence receiving component, the fluorescence receiving component includes an adjusting mechanism and a fluorescence receiver arranged side by side or stacked, and the fluorescence receiving component is In a long strip vertically placed, the adjustment mechanism extends to the side where the laser assembly is located.