CN104215562A - Optical system of particle analyzer - Google Patents
Optical system of particle analyzer Download PDFInfo
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- CN104215562A CN104215562A CN201310213994.0A CN201310213994A CN104215562A CN 104215562 A CN104215562 A CN 104215562A CN 201310213994 A CN201310213994 A CN 201310213994A CN 104215562 A CN104215562 A CN 104215562A
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Abstract
A disclosed optical system of a particle analyzer comprises a front light assembly, a flow chamber and a diffusion light collection assembly; the diffusion light collection assembly comprises a low-angle diaphragm, a low-angle photoelectric induction apparatus, a medium-angle diaphragm, a medium-angle photoelectric induction apparatus, a large-angle diaphragm and a large-angle photoelectric induction apparatus. On the detection basis of a conventional particle analyzer on low/medium-angle scope data particles, a diffusion light collection apparatus for detection of large-angle scope data particles is increased, so that detection on low/medium/large-angle scope data particles is realized, and three-dimensional data of a measured particle is obtained, and by collecting the diffusion light at a large-angle scope, more particle information can be obtained, and the particle classification accuracy is improved.
Description
Technical field
The application relates to the particle analyzer adopting light scattering method identification particle, particularly relates to the optical system of particle analyzer.
Background technology
No matter at clinical field or field of scientific study, Aulomatizeted Detect as fine particle classification such as cells has huge using value, and such as, blood cell analyzer as particle analyzer passes through information such as red blood cell, leucocyte and hematoblastic quantity, volumes in the identification of cell exportable blood of human body sample.
Different according to the principle counted detection of particles, method for classifying particles can be divided into impedance method and light scattering method usually.
Impedance method is based on Coulter principle, and for blood cell analyzer, impedance variation when instrument flows through aperture by measuring the blood cell in the electrolytic solution of suspending in aperture two end electrodes is carried out to haemocyte analysiss and counted.Number of pulses between the battery lead plate of detection energising is the cell quantity by aperture, and the intensity of pulse is directly proportional to the volume of cell.By Signal analysis and collection, in addition specific software systems and sorting algorithm both simply can be analyzed blood cell quantity and volume.The maximum deficiency of the method is only to hive off to haemocyte by the size of haemocyte, and leucocyte is divided into lymphocyte (LYM), monocyte (MONO), neutrophil leucocyte (NEU), eosinophil (EOS) and basophilic granulocyte (BASO) these five kinds.Because neutrophil leucocyte, eosinophil are similar with the volume of basophilic granulocyte, impedance method cannot measure the cell quantity that these three kinds have clinical meaning, therefore adopts the blood cell analyzer of impedance method to be commonly referred to as three and to hive off blood cell analyzer.
The deficiency that light scattering method can effectively avoid impedance method to bring, General Principle is as follows: blood sample preparatory unit flows to element of fluid after the sample after a certain amount of dilution and reagent being had an effect, then element of fluid transports the sample after this effect to the flow chamber in detecting unit, wherein flow chamber provides an optical detection region, sheath stream principle is used to be wrapped in sheath stream by blood cell sample stream in this region, blood cell is made one by one to pass through sense channel, light source in detecting unit, normally laser, an illumination beam is provided to be irradiated on the surveyed area of flow chamber, when cell flows through detection zone time, illumination beam is irradiated on cell will there is light scattering etc., by collecting the detection of two range of scatter angles inscattering light, and light signal is converted to electric pulse output, finally according to the size of pulse, two-dimentional scatter diagram is formed at two dimensional surface.
Wherein low angle (LAS) scattered light reflection cell volume size, and middle angle (MAS) scattered light reflection cell interior complexity, and according to two angle direction pulse strengties, each measurement cell is plotted on the scatter diagram of two dimension.Usually need to carry out twice sheath stream, first time is DIFF(Differential, leukocyte differential count) passage, this Measurement channel uses hemolytic agent process sample, and the DIFF scatter diagram of acquisition, as Fig. 1 (a), can by lymphocyte in DIFF scatter diagram, monocyte, Polymorphonuclear Leukocytes Nuclear, eosinophil distinguishes.Second time is BASO(Basophilic Granulocyte, basophilic granulocyte) passage, in the scatter diagram of this passage, separate basophilic granulocyte and other four kinds of leucocytes, as Fig. 1 (b).The blood cell analyzer of this Cleaning Principle is used to be called five classification blood cell analyzers.
The implementation method of usual optical system as shown in Figure 2, the laser beam that semiconductor laser 101 is launched is through collimation lens 102, 2 cylindrical mirrors 103, after 104 shapings, long elliptical beam is formed in flow chamber 105, excite the scattered light after leucocyte after scattered light collecting lens 106 collimates, the equal light beam of two beam intensities is divided into through beam splitter 107, corresponding scattering angle is sub-elected respectively through low angle diaphragm 108 and middle angle diaphragm 109, converge on respective detector 112 and 113 respectively through two plus lens 110, diaphragm for eliminating stray light 111 is had for eliminate stray light before detector.
Although adopt this optical system can distinguish five classification of cell, when testing the sample of eosinophilia, on DIFF scatter diagram, unclear boundaries between eosinophil and neutrophil leucocyte, affects Classification and Identification, brings clinical risk.In this case, if high angle scattered light can be detected, obtain the detailed information of more cell interior, obtain the data of basic, normal, high three angles, will the accuracy improving eosinophil identification be conducive to.But its scattered light collecting lens of optical system conventional is at present subject to the restriction of numerical aperture (NA), the scattering angle upper limit of collected scattered light is difficult to be greater than 20 °, therefore high angle scattered light cannot be collected, and scattered light is divided into two by beam splitter, be also difficult to expand to three or three collect with the scattering angle of upper channel.
Summary of the invention
The application provide a kind of can detect low, in, the optical system of the data particle analyzer of large three angles.
For achieving the above object, the application provides a kind of optical system of particle analyzer, comprising:
Front optical assembly, for providing the light beam irradiating detected particle;
Flow chamber, for providing detected particle by the place of light beam irradiation;
Scattered light collection assembly, for receiving detected particle by the scattered light after beam excitation, described scattered light collection assembly comprises:
Low angle diaphragm, it is in the light path of the scattered light of the first angular range that the light hole of described low angle diaphragm is arranged on the angle of described scattered light optical axis;
Low angle light electrical induction device, it is arranged in the light path of scattered light outgoing after low angle diaphragm of the first angular range, for responding to the scattered light of the first angular range and exporting electric signal;
Middle angle diaphragm, it is in the light path of the scattered light of the second angular range that the light hole of described middle angle diaphragm is arranged on the angle of described scattered light optical axis;
Middle angular light electrical induction device, its scattered light being arranged on the second angular range through in outgoing after angle diaphragm light path on, for responding to the scattered light of the second angular range and exporting electric signal;
Wide-angle diaphragm, it is in the light path of the scattered light of angular extent that the light hole of described wide-angle diaphragm is arranged on the angle of described scattered light optical axis;
Wide-angle photoelectric induction device, it is arranged in the light path of scattered light outgoing after wide-angle diaphragm of angular extent, for responding to the scattered light of angular extent and exporting electric signal;
Described second angular range is greater than the first angular range, and angular extent is greater than the second angular range.
As the further improvement of the application, described low angle diaphragm and middle angle diaphragm are incorporated on the first aperture plate, first aperture plate is arranged in the plane vertical with scattered light optical axis, described wide-angle diaphragm is arranged relative to scattered light inclined light shaft, and described low angle light electrical induction device and corresponding with it the respectively low angle diaphragm of middle angular light electrical induction device and middle angle diaphragm be arranged in parallel.
In one embodiment, the shape of the light hole of described low angle diaphragm is the scattered round that centre is provided with the first angular range scattered light of shield bars, and the center of circle of the light hole of low angle diaphragm is positioned on optical axis.The shape of the light hole of described middle angle diaphragm is by straight flange, the shape that in the second angular range relative with described straight flange, the scattering circular arc of maximum angular scattered light and two parallel edges vertical with described straight flange enclose, the limit of described straight flange and low angle diaphragm light hole is at a distance of the first setpoint distance, first setpoint distance equals the difference of the scattered round radius of maximum angular scattered light in the scattered round radius of the second angular range minimum angle scattered light and the first angular range, have second between described two parallel edges and distance is set, second arranges the scattering diameter of a circle that distance is less than maximum angular scattered light in the second angular range.
In one embodiment, described wide-angle diaphragm becomes 30 ° ~ 60 ° settings relative to scattered light optical axis, and best angle is near 45 ° or 45 °, and described wide-angle photoelectric induction device and wide-angle diaphragm be arranged in parallel.
The application is by being separately positioned in the light path of low angle scattered light, middle angle scattered light and large-angle scattered light by three of correspondence diaphragms, thus achieve tested particle is excited after send low, in, the collection of large three kinds of angular range scattered lights, the three-dimensional data of tested particle can be obtained, by collecting the scattered light of polarizers of big angle scope, more particle information can be obtained, the accuracy of particle classifying can be improved.
Accompanying drawing explanation
Fig. 1 a and Fig. 1 b is respectively the two-dimentional scatter diagram that five classification blood cell analyzers are drawn through twice sheath stream;
Fig. 2 is a kind of index path of five classification blood cell analyzer optical systems;
Fig. 3 is the index path of the optical system of particle analyzer in a kind of embodiment of the application;
Fig. 4 be in a kind of embodiment of the application in, the structural representation of low angle diaphragm;
Fig. 5 be in a kind of embodiment of the application in, the structural representation of low angle light electrical induction device;
Fig. 6 be in a kind of embodiment of the application in, the assembling schematic diagram of low angle diaphragm and photoelectric induction device;
Fig. 7 is the structural representation of wide-angle diaphragm in a kind of embodiment of the application;
Fig. 8 is the wiring layout of high angle diaphragm and photoelectric induction device in the embodiment of the present application;
Fig. 9 is the assembling schematic diagram of the first structural member and the second structural member in a kind of embodiment of the application;
Figure 10 be in a kind of embodiment of the application the first structural member and the second structural member assembling after structural representation;
Figure 11 a is wide-angle photoelectric induction device rigging position comparison diagram in a kind of embodiment of the application;
Figure 11 b is the response of photoelectric induction device to incident light and the graph of a relation of incident angle;
Figure 12 is the one-piece construction schematic diagram of optical system in a kind of embodiment of the application;
Figure 13 a is the three-dimensional scatter diagram of DIFF passage in a kind of embodiment of the application;
Figure 13 b is the three-dimensional scatter diagram of BASO passage in a kind of embodiment of the application;
Figure 14 a to project two the two-dimentional scatter diagrams obtained in both direction for three-dimensional scatter diagram shown in Figure 13 a;
Figure 14 b to project two the two-dimentional scatter diagrams obtained in both direction for three-dimensional scatter diagram shown in Figure 13 b.
Embodiment
Also by reference to the accompanying drawings the application is described in further details below by concrete embodiment.
Tested particle is sent scattered light by after beam excitation, in the embodiment of the present application, collect the scattered light of three angular ranges, wherein angular extent is greater than the second angular range, second angular range is greater than the first angular range, namely the scattered light of the first angular range is low-angle scattered light, the scattered light of the second angular range is the scattered light of middle angle, the scattered light of angular extent is the scattered light of wide-angle, first, two, between angular extent, not certain requirement continuously, but the interval of set angle can be had, such as, first angular range is 0 °-8 °, second angular range is 10 °-20 °, angular extent is 23 °-40 °.Three diaphragms are set in the embodiment of the present application, collect the scattered light of three angular ranges respectively, being arranged on the angle of described scattered light optical axis by the light hole of low angle diaphragm and low angle light electrical induction device is in the light path of the scattered light of the first angular range, for collecting the scattered light of the first angular range.Being arranged on the angle of described scattered light optical axis by the light hole of middle angle diaphragm and middle angular light electrical induction device is in the light path of the scattered light of the second angular range, for collecting the scattered light of the second angular range.Being arranged on the angle of described scattered light optical axis by the light hole of wide-angle diaphragm and wide-angle photoelectric induction device is in the light path of the scattered light of angular extent, for collecting the scattered light of angular extent.
In a specific embodiment of the application, for reducing the area of photoelectric induction device, low angle diaphragm and middle angle diaphragm are positioned at same plane, this plane orthogonal is in scattered light optical axis, and wide-angle diaphragm is arranged relative to scattered light inclined light shaft, the scattered light of angular extent is incided on photoelectric induction device to by wide-angle diaphragm with large incident angles of trying one's best.
Be that blood cell analyzer is specifically described the application for example below with particle analyzer, it will be appreciated by those skilled in the art that particle analyzer can also be the instrument of other application light scattering method identification particle.
The optical system schematic diagram of particle analyzer as shown in Figure 3, the optical system of blood cell analyzer comprises front optical assembly 905, flow chamber 105 and scattered light collection assembly 906.Front optical assembly 905 irradiates the light beam of detected blood cell for providing; Flow chamber 105 is for providing detected cell by the place of light beam irradiation, and under normal circumstances, flow chamber 105 is one to be had and can sweep along the transparent chamber of the aperture that cell passes through for sheath fluid; Scattered light collection assembly 906, for directly receiving detected particle by the scattered light after beam excitation, does not collimate scattered light between scattered light collection assembly 906 and flow chamber 105, assembles and/or the optical device of Shape correction.
In a kind of instantiation, front optical assembly 905 comprises the cylindrical mirror 103 and 104 of laser generator 101 and the collimation lens arranged along laser optical path 102, two vertical placement mutually, and laser generator 101 can be such as laser diode.Flow chamber 105, between cylindrical mirror and scattered light collection assembly 906, after the cylindrical mirror 103 that collimation lens 102, two is vertically placed mutually and 104 pairs of laser carry out collection convergence, forms hot spot at flow chamber 105 place.Scattered light collection assembly 906 comprises low angle diaphragm 201, low angle light electrical induction device 202, middle angle diaphragm 203, middle angular light electrical induction device 204, wide-angle diaphragm 205 and wide-angle photoelectric induction device 206, wherein, low angle diaphragm 201 and middle angle diaphragm 203 are incorporated on the first aperture plate 301, as shown in figs. 3 and 6, the first aperture plate 301 is arranged in the plane vertical with scattered light optical axis.In other instantiation, low angle diaphragm 201 and middle angle diaphragm 203 also can not be disposed on the same plane, and can have certain angle or be arranged in parallel between low angle diaphragm 201 and middle angle diaphragm 203.In the present embodiment, low angle light electrical induction device 202 and middle angular light electrical induction device 204 also can integrate, and low angle diaphragm 201 corresponding with it respectively and middle angle diaphragm 203 be arranged in parallel.Wide-angle diaphragm 205 is that 30 ° of-60 ° of angles are arranged relative to scattered light optical axis; Wide-angle photoelectric induction device 206 and wide-angle diaphragm 205 be arranged in parallel.
After flow chamber vertical optical axis each plane on, different scattering angle form the scattering concentric circles of different-diameter at grade, by placing diaphragm on this plane, processing the perforate of different-diameter, the angular range of scattered light after incident diaphragm can be controlled.In the present embodiment, low angle diaphragm 201 and middle angle diaphragm 203 are arranged on the same plane perpendicular to optical axis, the scattered light of the corresponding angle scope that the size of the light hole of two diaphragms is passed through according to it and diaphragm to flow chamber distance and determine, in the present embodiment, defining the first angular range is 1 °-4 °, second angular range is 6 °-20 °, angular extent is 22 °-42 °, after low angle diaphragm 201 and middle angle diaphragm 203 place plane are determined to the distance of flow chamber, the size of the light hole of low angle diaphragm 201 and middle angle diaphragm 203 also can be determined.
Be illustrated in figure 4 the front view of low angle diaphragm 201 and middle angle diaphragm 203 place plane, the optical axis of system is by dot-and-dash line right-angled intersection point, the shape of the light hole of low angle diaphragm 201 is the scattered round that centre is provided with the first angular range scattered light of shield bars 401, in the present embodiment, the light hole of low angle diaphragm 201 is the scattered round 403 that centre is provided with 4 ° of scattered lights of shield bars 401, the center of circle of the light hole of low angle diaphragm 201 is positioned on optical axis, light on optical axis and the light very little with optical axis included angle are blocked in the effect of shield bars, because on optical axis, the intensity of the scattered light that the light intensity of laser sends much larger than cell, for reducing error, light on filtering optical axis and the light very little with optical axis included angle.The width of shield bars is determined according to the minimum angles in the first angular range, and in the present embodiment, the width of shield bars equals 1 ° of scattered light scattering diameter of a circle, thus low angle diaphragm 201 can be made by the scattered light of 1 ° ~ 4 °.
The light hole of middle angle diaphragm 203 is by the area of photoelectric induction device and the impact of shape, its shape can for the shape enclosed by scattering circular arc 404 and two parallel edges 405,406 vertical with straight flange 402 of maximum angular scattered light in straight flange 402, the second angular range relative with straight flange 402, wherein the scattered round of straight flange 402 and the second angular range minimum angle scattered light is tangent, in the present embodiment, the scattered round of straight flange 402 and 6 ° of scattered lights is tangent, scattering circular arc 404 is 20 ° of scattering circular arcs, thus can realize 6 ° ~ 20 ° scattered lights by middle angle diaphragm 203.Because of the light-sensitive surface normally rectangle of photoelectric induction device, in order to as far as possible many by scattered light, between two parallel edges, there is distance H, in the present embodiment, H length is determined by the light-sensitive surface size of wide-angle photoelectric induction device thereafter, and H is larger, receives scattered signal more complete, and signal intensity is larger.This shape of the light hole of middle angle diaphragm 203 is more adaptive with the photoelectric induction device of rectangle.Certainly, those skilled in the art are according to content disclosed in the present application, also straight flange 402 can be extended to two parallel edges 405,406 disappearances and straight flange 402 directly connects with scattering circular arc 404, namely, the shape of the light hole of angle diaphragm 203 is the shape enclosed by straight flange 402 and scattering circular arc 404.
As shown in Figure 5,6, the middle low angle light electrical induction device 501 that low angle light electrical induction device 202 and middle angular light electrical induction device 204 integrate such as to comprise at rectangular photodiode bin A and the bin B that certain interval is left in large and centre, the low angle diaphragm in the first aperture plate 301 and middle angle diaphragm aim at respectively in the bin B of angular light electrical induction device and the bin A of low angle light electrical induction device.Middle low angle light electrical induction device 501 adopts chip ceramic to encapsulate, and is directly welded on pcb board 502, and there is signal output terminal at pcb board 502 back side, exports low angle and middle scattered through angles light signal to amplifying circuit by signal wire.First aperture plate 301, middle low angle light electrical induction device 501 are assembled on the first structural member 601 by screw, and the first structural member 601 is assembled to has certain distance with flow chamber 105 and perpendicular in the plane of optical axis.
As shown in Figure 7, wide-angle diaphragm 205 perforate is rectangle, passes through with the high angle scattered light realizing in surface level 22 ° ~ 42 °.Wide-angle diaphragm 205 adopts chip ceramic to encapsulate, and be directly welded on pcb board 701, there is signal output terminal at the pcb board back side, exports large angle scattering light signal to amplifying circuit by signal wire.
As shown in Figure 8, wide-angle diaphragm 205 and wide-angle photoelectric induction device 206 are assembled on the second structural member 801 by screw.As shown in Figure 9, second structural member 801 has and wide-angle diaphragm fillet surface 802 at an angle, second structural member 801 is connected with the first structural member 601 by screw, when connecting, fillet surface on second structural member 801 is fitted on the first aperture plate 301, to make wide-angle diaphragm 205 and scattered light optical axis angled, the first structural member 601 after assembling and the second structural member 801 are as shown in Figure 10.Therefore by the chamfering number of degrees of this fillet surface 802, the angle of wide-angle diaphragm 205 and scattered light optical axis can be controlled.Certainly, the first structural member and the second structural member also can be processed into integrated structure.In the present embodiment, wide-angle diaphragm 205 and wide-angle photoelectric induction device 206 are all placed relative to scattered light inclined light shaft 45° angle or the 45° angle that substantially tilts (namely 45 ° near angle), and this is conducive to reducing the photosensitive area of wide-angle photoelectric induction device and improving the photoresponse of wide-angle photoelectric induction device.
As shown in fig. lla, the photoelectric induction device 206a that the wide-angle photoelectric induction device 206 inclination 45 ° placed and vertical optical axis are placed compares, and analyzes the photoresponse of different inclination angle.The relation curve of the normalized response of typical light electric diode and incident ray and light-sensitive surface angle is as mistake! Do not find Reference source.Shown in 11b, the response of light vertical incidence is maximum, and oblique incidence response reduces rapidly.With Figure 11 a for schematic diagram, if flow chamber endoporus cell scattering angle is θ, flow chamber endoporus sheath fluid refractive index is n, and after flow chamber refraction, the incident angle of scattered light incident light electrical induction device 206 and photoelectric induction device 206a is respectively i
1and i
2.Then
i
1=min[135°-arcsin(nsinθ),45°+arcsin(nsinθ)]
i
2=90°-arcsin(nsinθ)
Large angle scattering angular region is between 22 ° ~ 42 °, and the refraction angle after outgoing flow chamber is very large.Bring formula above into for 30 ° of scattering angle and flow chamber endoporus sheath fluid refractive index n=1.33269, obtain i
1=86.79 °, i
2=41.79 °.Placing its response of photoelectric induction device 206a by the known vertical optical axis of 11b is 30% of the wide-angle photoelectric induction device 206 that inclination 45 ° is placed, and other high angle scattered lights are also substantially like this.Arrange wide-angle diaphragm 205 and wide-angle photoelectric induction device 206 in visible the present embodiment to be 45 ° with optical axis and to place, significantly can improve the response of large-angle scattered light, reduce the gain of wide-angle signalling channel photoamplifier circuit, restraint speckle, improve circuit signal to noise ratio (S/N ratio).
Certainly, it will be appreciated by those skilled in the art that wide-angle diaphragm 205 and wide-angle photoelectric induction device 206 also can be other angle values with the angle of optical axis, such as 40 °, 43 °, 47 ° or 50 °.
Overall optical system as shown in figure 12, comprise front optical assembly 905, rectifier stack 904, flow chamber 105, flow chamber adjusting slider 903, scattered light collection assembly 906, substrate 901, beam 902, four Rubber shock-absorbing pads 902 below substrate 901 play anti-vibration and shock effect, to protect each optical element on optical substrate; Flow chamber 105 and rectifier stack 904 are assembled together, and are together arranged on flow chamber adjusting slider 903 with scattered light collection assembly 906.
It should be noted that, scattered light collecting lens is not adopted in the embodiment of the present application, scattered light collection assembly is direct receiving scattered light, cell is directly arrived low angle diaphragm by the scattered light sent after beam excitation, middle angle diaphragm and wide-angle diaphragm, namely the scattered light that sends of cell is without collimation, assemble and/or Shape correction and directly arrive low angle diaphragm, middle angle diaphragm and wide-angle diaphragm, and by low angle diaphragm, directly low angle light electrical induction device is arrived respectively after middle angle diaphragm and wide-angle diaphragm, middle angular light electrical induction device and wide-angle photoelectric induction device, therefore the structure of scattered light collection light path is simplified, significantly shorten optical path length, reduce optical system volume, the size of optical system is much smaller than existing five classification blood cell analyzer optical systems, approximately be only 1/4 of existing five classification blood cell analyzer optical systems.
For blood cell analyzer, the specific works process of present embodiment is, produce laser by laser generator 101, through collimation non-spherical lens 102 by laser beam datum, after converge in flow chamber 105 through mutual vertical cylindrical mirror 103 and 104 shaping of placing.During measurement count, liquid-way system injects in flow chamber 105 by after agent treated containing leukocytic sample liquid, and sample liquid is compressed to flow chamber 105 endoporus central narrow region by sheath fluid parcel and flows fast through laser-irradiated domain, sends scattered light.Scattered light outgoing after flow chamber 105 reflects, assemble without scattered light collecting lens, be directly incident on three diaphragms and photoelectric induction device that flow chamber places below, detect respectively low, in, the scattered light signal of large three angular ranges, and to export after light signal is transformed electric impulse signal.During operation, the position of fine adjustment flow chamber 105 and the first structural member 601 and the second structural member 801, makes it reach angle design requirement.
The dual channel approaches adopting DIFF+BASO is measured in Arneth's count, namely within a measuring period, successively carrying out DIFF and BASO passage twice sheath stream pushes away sample sequential, three-dimensional DIFF and BASO scatter diagram can be generated, be the three-dimensional scatter diagram of DIFF passage as depicted in fig. 13 a, Figure 13 b is depicted as the three-dimensional scatter diagram of BASO passage.The three-dimensional scatter diagram of Figure 13 a and 13b is projected in both direction, two two-dimentional scatter diagrams can be obtained respectively, as shown in Figure 14 a and Figure 14 b, wherein the DIFF scatter diagram information of Figure 14 a is the abundantest, is responsible for lymphocyte, monocyte, eosinophil, Polymorphonuclear Leukocytes Nuclear and paracytic classification; The BASO scatter diagram of Figure 14 b is responsible for basophilic granulocyte and paracytic classification.Visible, in the DIFF scatter diagram of high eosinophil sample, LAS-WAS (high angle) projecting direction eosinophil and Polymorphonuclear Leukocytes Nuclear boundary obviously, are easy to the two to make a distinction.
Above content is the further description done the application in conjunction with concrete embodiment, can not assert that the concrete enforcement of the application is confined to these explanations.For the application person of ordinary skill in the field, under the prerequisite not departing from the application's design, some simple deduction or replace can also be made, all should be considered as the protection domain belonging to the application.
Claims (9)
1. the optical system of particle analyzer, comprising:
Front optical assembly, for providing the light beam irradiating detected particle;
Flow chamber, for providing detected particle by the place of light beam irradiation;
Scattered light collection assembly, for receiving detected particle by the scattered light after beam excitation, is characterized in that described scattered light collection assembly comprises:
Low angle diaphragm, it is in the light path of the scattered light of the first angular range that the light hole of described low angle diaphragm is arranged on the angle of described scattered light optical axis;
Low angle light electrical induction device, it is arranged in the light path of scattered light outgoing after low angle diaphragm of the first angular range, for responding to the scattered light of the first angular range and exporting electric signal;
Middle angle diaphragm, it is in the light path of the scattered light of the second angular range that the light hole of described middle angle diaphragm is arranged on the angle of described scattered light optical axis;
Middle angular light electrical induction device, its scattered light being arranged on the second angular range through in outgoing after angle diaphragm light path on, for responding to the scattered light of the second angular range and exporting electric signal;
Wide-angle diaphragm, it is in the light path of the scattered light of angular extent that the light hole of described wide-angle diaphragm is arranged on the angle of described scattered light optical axis;
Wide-angle photoelectric induction device, it is arranged in the light path of scattered light outgoing after wide-angle diaphragm of angular extent, for responding to the scattered light of angular extent and exporting electric signal;
Described second angular range is greater than the first angular range, and angular extent is greater than the second angular range.
2. the optical system of particle analyzer as claimed in claim 1, it is characterized in that, described low angle diaphragm and middle angle diaphragm are incorporated on the first aperture plate, first aperture plate is arranged in the plane vertical with scattered light optical axis, described wide-angle diaphragm is arranged relative to scattered light inclined light shaft, and described low angle light electrical induction device and corresponding with it the respectively low angle diaphragm of middle angular light electrical induction device and middle angle diaphragm be arranged in parallel.
3. the optical system of particle analyzer as claimed in claim 2, it is characterized in that, the shape of the light hole of described low angle diaphragm is the scattered round that centre is provided with the first angular range scattered light of shield bars, and the center of circle of the light hole of low angle diaphragm is positioned on optical axis.
4. the optical system of particle analyzer as claimed in claim 3, it is characterized in that, the shape of the light hole of described middle angle diaphragm is the shape enclosed by the scattering circular arc of maximum angular scattered light in straight flange, the second angular range relative with described straight flange and two parallel edges vertical with described straight flange, and the scattered round of described straight flange and the second angular range minimum angle scattered light is tangent; Or the shape of the light hole of described middle angle diaphragm is the shape enclosed by the scattering circular arc of maximum angular scattered light in straight flange and the second angular range, the scattered round of described straight flange and the second angular range minimum angle scattered light is tangent.
5. the optical system of particle analyzer as claimed in claim 4, it is characterized in that, the width of described shield bars equals 1 ° of scattered light scattering diameter of a circle, first angular range is 1 ° ~ 4 °, second angular range is 6 ° ~ 20 °, angular extent is 22 ° ~ 42 °, described low angle light electrical induction device, middle angular light electrical induction device and wide-angle photoelectric induction device comprise the photodiode bin of rectangle respectively, and described low angle light electrical induction device and middle angular light electrical induction device integrate.
6. the optical system of the particle analyzer according to any one of claim 2-5, is characterized in that, described wide-angle diaphragm becomes 30 ° ~ 60 ° angles to arrange relative to scattered light optical axis, and described wide-angle photoelectric induction device and wide-angle diaphragm be arranged in parallel.
7. the optical system of particle analyzer as claimed in claim 6, is characterized in that, described wide-angle diaphragm is arranged relative to scattered light optical axis angle at 45 ° or angle substantially at 45 °.
8. the optical system of particle analyzer as claimed in claim 7, it is characterized in that, also comprise the first structural member and the second structural member, described first aperture plate, low angle light electrical induction device and middle angular light electrical induction device are arranged on the first structural member, wide-angle diaphragm and wide-angle photoelectric induction device are arranged on the second structural member, it is the fillet surface of 45 ° that described second structural member has with wide-angle diaphragm, described fillet surface is fitted on the first aperture plate, with make wide-angle diaphragm and scattered light optical axis at 45 °.
9. the optical system of the particle analyzer according to any one of claim 1 to 8, it is characterized in that, detected particle is directly arrived low angle diaphragm, middle angle diaphragm and wide-angle diaphragm by the scattered light sent after beam excitation, and by directly arriving low angle light electrical induction device, middle angular light electrical induction device and wide-angle photoelectric induction device respectively after low angle diaphragm, middle angle diaphragm and wide-angle diaphragm.
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| CN201310213994.0A CN104215562A (en) | 2013-05-31 | 2013-05-31 | Optical system of particle analyzer |
| CN202011322064.5A CN112485167A (en) | 2013-05-31 | 2013-05-31 | Optical system of particle analyzer |
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| CN201310213994.0A CN104215562A (en) | 2013-05-31 | 2013-05-31 | Optical system of particle analyzer |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110617804A (en) * | 2019-09-25 | 2019-12-27 | 浙江海洋大学 | Marine ecological environment detection system and method based on remote sensing technology |
| CN112730203A (en) * | 2020-12-29 | 2021-04-30 | 深圳市科曼医疗设备有限公司 | Optical system of blood cell analyzer, optical gain calibration method and storage medium |
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| CN104949910A (en) * | 2015-05-29 | 2015-09-30 | 广州埃克森生物科技有限公司 | 5-Part differential hematology analyzer optical system |
| CN105203446A (en) * | 2015-09-14 | 2015-12-30 | 广州埃克森生物科技有限公司 | Cell classified statistic method based on probability distribution |
| CN108139310B (en) * | 2016-01-06 | 2020-11-24 | Lg伊诺特有限公司 | Particle sensing device |
| CN108139310A (en) * | 2016-01-06 | 2018-06-08 | Lg伊诺特有限公司 | Particle sensing device further |
| CN106885806A (en) * | 2016-03-04 | 2017-06-23 | 摩瑞尔电器（昆山）有限公司 | A kind of big data system and method based on laser detection blood |
| CN106198359B (en) * | 2016-07-05 | 2019-08-06 | 迪瑞医疗科技股份有限公司 | A kind of Arneth's count instrument optical system using integrating sphere |
| CN106198359A (en) * | 2016-07-05 | 2016-12-07 | 长春迪瑞医疗科技股份有限公司 | A kind of differential blood count instrument optical system using integrating sphere |
| CN109580550A (en) * | 2018-12-03 | 2019-04-05 | 迪瑞医疗科技股份有限公司 | A kind of classification processing method and its device of leucocyte |
| CN113196039A (en) * | 2019-01-14 | 2021-07-30 | 深圳迈瑞生物医疗电子股份有限公司 | Sample optical detection device, sample detection method and sample analyzer |
| CN110617804A (en) * | 2019-09-25 | 2019-12-27 | 浙江海洋大学 | Marine ecological environment detection system and method based on remote sensing technology |
| CN112881720A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sample analyzer, laser module |
| WO2021104500A1 (en) * | 2019-11-30 | 2021-06-03 | 深圳市帝迈生物技术有限公司 | Sample analyzer and laser assembly |
| WO2022068630A1 (en) * | 2020-09-30 | 2022-04-07 | 深圳市帝迈生物技术有限公司 | Optical measurement apparatus and sample analyzer |
| CN112730203A (en) * | 2020-12-29 | 2021-04-30 | 深圳市科曼医疗设备有限公司 | Optical system of blood cell analyzer, optical gain calibration method and storage medium |
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