CN203132991U - Movable-part-free multichannel angle modulation type surface plasmon resonance (SPR) sensor detection system - Google Patents
Movable-part-free multichannel angle modulation type surface plasmon resonance (SPR) sensor detection system Download PDFInfo
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- CN203132991U CN203132991U CN 201320132830 CN201320132830U CN203132991U CN 203132991 U CN203132991 U CN 203132991U CN 201320132830 CN201320132830 CN 201320132830 CN 201320132830 U CN201320132830 U CN 201320132830U CN 203132991 U CN203132991 U CN 203132991U
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Abstract
The utility model discloses a movable-part-free multichannel angle modulation type surface plasmon resonance (SPR) sensor detection system. The system comprises an SPR sensor and a miniature flow cell which is attached to the detection surface of the SPR sensor, wherein the SPR sensor comprises a light transmitting component, a light receiving component and a light reflecting component for receiving the light of the light transmitting component and reflecting the light to the light receiving component, and the miniature flow cell is a multichannel miniature flow cell. The parallel light rays transmitted by the light transmitting component enter from a cylindrical lens and then are converged to a metal film part on a semi-cylindrical prism, and the reflected light is projected onto an optical sensor through an imaging lens. The SPR sensor detection system can realize reference and specific measurements so as to improve the measurement sensitivity of a detector, and has the advantages of simplicity in mechanical structure, convenience in assembling and debugging, high sensitivity, stable performance, miniaturization and high performance cost ratio.
Description
Technical field
The present invention relates to field of biochemistry detection, relate in particular to a kind of surface plasma resonance sensor detection system.
Background technology
Surface plasma body resonant vibration SPR (Surface plasmon resonance) phenomenon, the free electron that the evanescent wave that the p polarized light produces when glass and metallic film intersection generation total reflection can cause the metal surface produces surface plasma (Surface Plasmon, SP), when the frequency of surface plasma and evanescent wave is identical with wave number, just can produce surface plasma resonance.For angular modulation type SPR, this phenomenon can make the intensity of reflected light of a certain incident angle reduce suddenly, and very responsive to the refractive index of metallic film surface dielectric.Since eighties of last century SPR phenomenon is found, the SPR technology is widely used in monitoring fields such as biochemistry, medical treatment, food, environment over past ten years, becomes the focus of sensor research field.
Spr sensor is very responsive to its surperficial medium, and the small variation of the sensor surface fluid refracting rate of flowing through just can cause the variation of sensor output, and its measuring process sample consumption is not more than 1ul.The SPR detection technique has characteristics such as detection sensitivity height, anti-electromagnetic interference performance is good, the sample consumption is few.
The spr sensor of angular modulation roughly has two classes at present, and a class is the angle scanning sensor, adopts mechanical movable member such as movable incident wall or galvanometer to wait to adjust the angle of incident light.This sensor accuracy is subject to mechanical part, and finishes once complete angle scanning and need the regular hour, and temporal resolution is low.Another kind of is by optical element incident light to be converged on the sensitive face, makes the information that contains different incidence angles in the reflected light like this.This sensor has higher requirements to the light path precision if incident light converges on the sensitive face, and the light path adjustment often is not very convenient.If the incident light focus is on sensitive face, then the diverse location correspondence the light of different incident angles on the sensitive face, and this can have higher requirements to metallic film flatness of whole sensitive face.
Summary of the invention
The invention provides that a kind of light path is debug conveniently, the hyperchannel angular modulation type surface plasma resonance sensor detection system of the no movable member of highly sensitive, stable performance.
A kind of hyperchannel angular modulation type spr sensor detection system of not having movable member, comprise spr sensor and the miniature flow cell of fitting and arranging with the detection faces of described spr sensor, described spr sensor comprises light emission component, optical fiber receive module and is used for receiving the light of light emission component and it is reflexed to the light reflection subassembly of optical fiber receive module;
Described light emission component comprises light source, collimation lens, polaroid, filter plate and the rectangular aperture that sets gradually along radiation direction;
Described smooth reflection subassembly comprises first cylindrical lens, semicolumn prism, second cylindrical lens that sets gradually along the emergent ray direction of light emission component;
Wherein be coated with the layer of metal film on the reflective surface of semicolumn prism, the focal line of first cylindrical lens and second cylindrical lens all coincides with on the center of circle line of semicolumn prism, is positioned at the metallic film place;
Described optical fiber receive module comprises first imaging len, second imaging len and the optical sensor that sets gradually along the emergent ray direction of light reflection subassembly;
Described miniature flow cell is multichannel miniature flow cell.
The p light that the polarization direction of the polaroid in the above-mentioned light emission component is arranged to only allow to participate in the SPR reaction passes through.
The parallel rays that light emission component sends converges on the center of circle line of semicolumn prism after the first cylindrical lens incident, be on the axis of semicolumn prism, second cylindrical lens that reflexes to opposite side through metallic film becomes directional light output again, projects on the optical sensor finally by crossing first and second imaging lens.
The focal line of first cylindrical lens and second cylindrical lens overlaps with the center of circle line of semicolumn prism, the parallel beam that light emission component sends converges light all perpendicular to the grazing incidence of semicolumn prism after by first cylindrical lens, enter the semicolumn prism after radiation direction do not change.
The reflective surface of metallic film is towards the semicolumn prism in the utility model spr sensor, and fluid to be measured is through miniature flow cell metallic film semicolumn prism one side dorsad of flowing through, and as preferably, described metallic film is golden film, silverskin or gold and silver composite membrane.
Further preferred, be modified with the special film to different test substance sensitivities on the metallic film surface, can realize the highly sensitive detection to predetermined substance in the sample.
As preferably, described optical sensor is area array CCD or face battle array CMOS.Wherein, ccd sensor all is better than cmos sensor at aspects such as sensitivity, resolution, noise controls, and cmos sensor then has the characteristics of low cost, low-power consumption and high degree of integration, selects according to concrete needs during operation.
As preferably, described light source is led light source.The heat that led light source produces seldom, by electric energy to the conversion ratio of luminous energy near 100%, can not cause the waste of the energy, and led light source do not use mercury, not leaded yet, have the feature of environmental protection, be suitable for the detection of biological sample.
The miniature flow cell that is used with above-mentioned spr sensor in the utility model is multichannel flow cell, as preferably, air reference groove and U-shaped circulation groove are carved with in described miniature flow cell surface, and the two ends of U-shaped circulation groove also are respectively equipped with at least one inlet opening and a fluid hole; When the metal foil face of described miniature flow cell and spr sensor was fitted, air reference groove and U-shaped circulation groove were formed air duct and liquid communication passage with metallic film respectively.
During the work of the utility model spr sensor detection system, spr sensor and miniature flow cell are fitted and are arranged, when solution to be measured is flowed through spr sensor by miniature flow cell, this sensor passes through the detection of intensity of reflected light and then the refractive index of evaluating objects solution, realization is to the detection of testing sample, in conjunction with the modification to the sensor detection faces, can realize the specific detection to predetermined substance simultaneously.Spr sensor of the present utility model and corresponding miniature flow cell can be realized the measurement of reference mode and specificity mode, to improve the measurement sensitivity of detecting device.Have that physical construction is simple, assembling and setting convenient, highly sensitive, stable performance, miniaturization and the high advantage of cost performance.
Description of drawings
Fig. 1 is the index path of spr sensor in the utility model;
Fig. 2 is the physical construction synoptic diagram of spr sensor in the utility model;
Fig. 3 is the cylindrical lens prism stuck-module synoptic diagram of spr sensor in the utility model;
Fig. 4 is the structural representation of miniature flow cell in the utility model.
Embodiment
Be illustrated in figure 1 as the hyperchannel angular modulation type surface plasma resonance sensor of no movable member in the utility model, comprise light emission component, optical fiber receive module and be used for receiving the light of light emission component and it is reflexed to the light reflection subassembly of optical fiber receive module,
Light emission component is provided with light source 1, pin hole 2, collimation lens 3, polaroid 4, filter plate 5 and rectangular aperture 6 successively along radiation direction; The p light that the polarization direction of polaroid 4 is arranged to only allow to participate in the SPR reaction passes through, and light source wherein adopts led light source.
The light reflection subassembly comprises first cylindrical lens 7, semicolumn prism 8, second cylindrical lens 10 that sets gradually along the emergent ray direction of light emission component; The focal line that wherein is coated with layer of metal film 9, the first cylindrical lenses 7 and second cylindrical lens 10 on the reflective surface of semicolumn prism 8 all coincides with on the center of circle line of semicolumn prism 8, is positioned at metallic film 9 places.
Optical fiber receive module is provided with the optical sensor 13 of first imaging len 11, second imaging len 12 and reception spr signal successively along radiation direction.
The parallel rays that light emission component sends converges to the metallic film 9 on semicolumn prism 8 focal lines after 7 incidents of first cylindrical lens, second cylindrical lens 10 that reflexes to opposite side through metallic film 9 becomes directional light output again, project on the optical sensor 13 finally by crossing first imaging len 11 and second imaging len 12, optical sensor among this embodiment adopts area array CCD, also can adopt face battle array CMOS as required certainly.
Fig. 2 is the physical construction synoptic diagram of spr sensor among Fig. 1, and as can be seen from Figure, light emission component also comprises LED lens barrel 14, collimation lens lens barrel 15, filter plate polaroid lens barrel 16.The light reflection subassembly comprises cylindrical lens prism stuck-module.Optical fiber receive module comprises imaging len lens barrel 17 and optical sensor 13.
Cooperation by internal and external threads between each parts interconnects, and the optical axis of guaranteeing each part optical element is coaxial.
As shown in Figure 3, the cylindrical lens prism stuck-module of spr sensor comprises semicolumn prism 8, the first cylindrical lens lens barrel 18, the second cylindrical lens lens barrel 20, web member 19 and prism fixture 21.The first cylindrical lens lens barrel 18 and the second cylindrical lens lens barrel 20 are fixing by screw and web member (19), and semicolumn prism 8 is fixed on 21 li of prism fixtures, and does in the groove of as a whole insertion web member 19.This design has guaranteed that the focal line of first cylindrical lens 7 overlaps with the center of circle line of semicolumn prism 8, and the directional light that light incident assembly sends can converge in metallic film 9 places through first cylindrical lens 7.
Fig. 4 is the structural representation of the multi-channel miniature flow cell that is used with spr sensor, and wherein the multi-channel miniature flow cell is made by the PDMS material.Be carved with air reference groove 22 and U-shaped circulation groove 23 on the flow cell, the inlet opening 24 of U-shaped groove and fluid hole 25 can insert for kapillary.This face that flow cell is carved with groove is close to metallic film 9 surfaces, the air signal of air reference groove 22 can be gathered the fluctuation of calibration light source 1 light intensity, detected solution is flowed into by inlet opening 24, flow through metallic film 9 surfaces along U-shaped circulation groove 23, thereby realize the spr signal of solution is measured, discharge from fluid hole 25 at last.Two arms of U-shaped circulation groove 23 can be used as two passes, by certain passage corresponding sensor detection faces is partly modified separately, two passes can suppress the influence that temperature variation causes the solution variations in refractive index to be brought as measuring passage and reference channel respectively in test, and realization reference mode is measured the sensitivity that improves sensor.Use the PDMS flow cell can protect the metallic film of sensor can not be damaged.
On metal film 9 surfaces, can modify different bio-sensitive films according to the difference of measurand, can realize the highly sensitive detection of predetermined substance in the sample, become the specific detection device.
During actual the use, the efferent duct of pump valve is connected to the inlet opening 24 in sensor circulation pond by kapillary, the biofilm surface of bio-modification (or through) flows out from fluid hole 25 flow of solution to be measured through metal film 9 surfaces.The sample liquid change of refractive on metallic film 9 surfaces, the concentration change that specific adsorption causes are converted to spectral signal in real time by spr sensor, can realize detecting by the analysis to this signal.
Claims (6)
1. the hyperchannel angular modulation type spr sensor detection system of not having movable member, comprise spr sensor and the miniature flow cell of fitting and arranging with the detection faces of described spr sensor, described spr sensor comprises light emission component, optical fiber receive module and is used for receiving the light of light emission component and it is reflexed to the light reflection subassembly of optical fiber receive module, it is characterized in that
Described light emission component comprises light source (1), collimation lens (3), polaroid (4), filter plate (5) and the rectangular aperture (6) that sets gradually along radiation direction;
Described smooth reflection subassembly comprises first cylindrical lens (7), semicolumn prism (8), second cylindrical lens (10) that sets gradually along the emergent ray direction of light emission component;
Wherein be coated with layer of metal film (9) on the reflective surface of semicolumn prism (8), the focal line of first cylindrical lens (7) and second cylindrical lens (10) all coincides with on the center of circle line of semicolumn prism (8), is positioned at metallic film (9) and locates;
Described optical fiber receive module comprises first imaging len (11), second imaging len (12) and the optical sensor (13) that sets gradually along the emergent ray direction of light reflection subassembly;
Described miniature flow cell is multichannel miniature flow cell.
2. the hyperchannel angular modulation type spr sensor detection system of no movable member as claimed in claim 1 is characterized in that described metallic film (9) is golden film, silverskin or gold and silver composite membrane.
3. the hyperchannel angular modulation type spr sensor detection system of no movable member as claimed in claim 1 is characterized in that, is modified with the special film to different test substance sensitivities on metallic film (9) surface.
4. the hyperchannel angular modulation type spr sensor detection system of no movable member as claimed in claim 1 is characterized in that, described optical sensor (13) is area array CCD or face battle array CMOS.
5. the hyperchannel angular modulation type spr sensor detection system of no movable member as claimed in claim 1 is characterized in that described light source is led light source.
6. the hyperchannel angular modulation type spr sensor detection system of no movable member as claimed in claim 1, it is characterized in that, air reference groove (22) and U-shaped circulation groove (23) are carved with in described miniature flow cell surface, and the two ends of U-shaped circulation groove (23) also are respectively equipped with at least one inlet opening (24) and a fluid hole (25); When metallic film (9) face of described miniature flow cell and spr sensor was fitted, air reference groove (22) and U-shaped circulation groove (23) were formed air duct and liquid communication passage with metallic film (9) respectively.
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| CN 201320132830 CN203132991U (en) | 2013-03-21 | 2013-03-21 | Movable-part-free multichannel angle modulation type surface plasmon resonance (SPR) sensor detection system |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439294B (en) * | 2013-09-09 | 2015-05-13 | 黑龙江大学 | Angle modulation and wavelength modulation surface plasmon resonance (SPR) sharing system |
| CN104713851A (en) * | 2015-03-24 | 2015-06-17 | 吉林大学 | Miniature real-time on-line offshore oil spill detection system |
| CN104792739A (en) * | 2015-04-14 | 2015-07-22 | 浙江大学 | SPR imaging sensor, adjusting method thereof and SPR imaging sensor chip |
| CN106442425A (en) * | 2016-12-19 | 2017-02-22 | 杭州晶百检测技术有限公司 | SPR bio-sensing chip |
| CN106525777A (en) * | 2016-12-19 | 2017-03-22 | 杭州晶百检测技术有限公司 | Handheld SPR detector and bio-sample detection method |
| CN106841120A (en) * | 2017-03-31 | 2017-06-13 | 丁利 | Suitable for the flow cell of multichannel terminal reflection type optical fiber spr sensor |
| CN106996924A (en) * | 2017-04-24 | 2017-08-01 | 徐州医科大学 | A kind of angle scanning type spr sensor system |
| CN108700513A (en) * | 2015-09-24 | 2018-10-23 | 拉克里赛恩斯有限责任公司 | Optical sensor, system and use its method |
| CN108827919A (en) * | 2018-04-30 | 2018-11-16 | 浙江诺迦生物科技有限公司 | A kind of portable SPR tester |
| CN110567919A (en) * | 2019-09-17 | 2019-12-13 | 华中科技大学 | Beam scanning type SPR sensor and use method thereof |
| CN111965143A (en) * | 2020-09-17 | 2020-11-20 | 广州大学 | Surface plasma resonance detection device |
| CN112630193A (en) * | 2020-12-18 | 2021-04-09 | 广州大学 | Angle modulation type SPR sensor based on digital micromirror device and SPR detection equipment |
-
2013
- 2013-03-21 CN CN 201320132830 patent/CN203132991U/en not_active Expired - Lifetime
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103439294B (en) * | 2013-09-09 | 2015-05-13 | 黑龙江大学 | Angle modulation and wavelength modulation surface plasmon resonance (SPR) sharing system |
| CN104713851A (en) * | 2015-03-24 | 2015-06-17 | 吉林大学 | Miniature real-time on-line offshore oil spill detection system |
| CN104792739A (en) * | 2015-04-14 | 2015-07-22 | 浙江大学 | SPR imaging sensor, adjusting method thereof and SPR imaging sensor chip |
| CN108700513A (en) * | 2015-09-24 | 2018-10-23 | 拉克里赛恩斯有限责任公司 | Optical sensor, system and use its method |
| CN106442425A (en) * | 2016-12-19 | 2017-02-22 | 杭州晶百检测技术有限公司 | SPR bio-sensing chip |
| CN106525777A (en) * | 2016-12-19 | 2017-03-22 | 杭州晶百检测技术有限公司 | Handheld SPR detector and bio-sample detection method |
| CN106841120B (en) * | 2017-03-31 | 2023-08-25 | 丁利 | Flow cell suitable for multichannel terminal reflection type optical fiber SPR sensor |
| CN106841120A (en) * | 2017-03-31 | 2017-06-13 | 丁利 | Suitable for the flow cell of multichannel terminal reflection type optical fiber spr sensor |
| CN106996924A (en) * | 2017-04-24 | 2017-08-01 | 徐州医科大学 | A kind of angle scanning type spr sensor system |
| CN106996924B (en) * | 2017-04-24 | 2023-12-19 | 徐州医科大学 | Angle scanning type SPR sensor system |
| CN108827919A (en) * | 2018-04-30 | 2018-11-16 | 浙江诺迦生物科技有限公司 | A kind of portable SPR tester |
| CN108827919B (en) * | 2018-04-30 | 2024-04-16 | 浙江诺迦生物科技有限公司 | Portable SPR tester |
| CN110567919A (en) * | 2019-09-17 | 2019-12-13 | 华中科技大学 | Beam scanning type SPR sensor and use method thereof |
| CN111965143A (en) * | 2020-09-17 | 2020-11-20 | 广州大学 | Surface plasma resonance detection device |
| CN112630193A (en) * | 2020-12-18 | 2021-04-09 | 广州大学 | Angle modulation type SPR sensor based on digital micromirror device and SPR detection equipment |
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Effective date of registration: 20180918 Address after: 311200 Hangzhou, Zhejiang, Hangzhou, Xiaoshan economic and Technological Development Zone 198 Hangzhou Bay Information Port E 16 block. Patentee after: ZHEJIANG NEOGENE BIOTECHNOLOGY CO.,LTD. Address before: 310027 No. 38, Zhejiang Road, Hangzhou, Zhejiang, Xihu District Patentee before: Zhejiang University |
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Granted publication date: 20130814 |
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